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Non-invasive strategies that can identify oral malignant and dysplastic oral potentially-malignant lesions (OPML) are necessary in cancer screening and long-term surveillance. Optical coherence tomography (OCT) can be a rapid, real time and non-invasive imaging method for frequent patient surveillance. Here, we report the validation of a portable, robust OCT device in 232 patients (lesions: 347) in different clinical settings. The device deployed with algorithm-based automated diagnosis, showed efficacy in delineation of oral benign and normal (n = 151), OPML (n = 121), and malignant lesions (n = 75) in community and tertiary care settings. This study showed that OCT images analyzed by automated image processing algorithm could distinguish the dysplastic-OPML and malignant lesions with a sensitivity of 95% and 93%, respectively. Furthermore, we explored the ability of multiple (n = 14) artificial neural network (ANN) based feature extraction techniques for delineation high grade-OPML (moderate/severe dysplasia). The support vector machine (SVM) model built over ANN, delineated high-grade dysplasia with sensitivity of 83%, which in turn, can be employed to triage patients for tertiary care. The study provides evidence towards the utility of the robust and low-cost OCT instrument as a point-of-care device in resource-constrained settings and the potential clinical application of device in screening and surveillance of oral cancer.

The ECM protein EFEMP1 (fibulin-3) is associated with all types of solid tumor through its cell context-dependent dual function. A variant of fibulin-3 was engineered by truncation and mutation to alleviate its oncogenic function, specifically the proinvasive role in glioblastoma multiforme (GBM) cells at stem-like state. ZR30 is an in vitro synthesized 39-kDa protein of human fibulin-3 variant. It has a therapeutic effect in intracranial xenograft models of human GBM, through suppression of epidermal growth factor receptor/AKT and NOTCH1/AKT signaling in GBM cells and extracellular MMP2 activation. Glioblastoma multiforme is highly vascular, with leaky blood vessels formed by tumor cells expressing endothelial cell markers, including CD31. Here we studied GBM intracranial xenografts, 2 weeks after intratumoral injection of ZR30 or PBS, by CD31 immunohistochemistry. We found a 70% reduction of blood vessel density in ZR30-treated xenografts compared with that of PBS-treated ones. Matrigel plug assays showed the effect of ZR30 on suppressing angiogenesis. We further studied the effect of ZR30 on genes involved in endothelial transdifferentiation (ETD), in 7 primary cultures derived from 3 GBMs under different culture conditions. Two GBM cultures formed mesh structures with upregulation of ETD genes shortly after culture in Matrigel Matrix, and ZR30 suppressed both. ZR30 also downregulated ETD genes in two GBM cultures with high expression of these genes. In conclusion, multifaceted tumor suppression effects of human fibulin-3 variant include both suppression of angiogenesis and vasculogenic mimicry in GBM.

In this article, we look back on history of the classification of tumors of central nervous system (CNS) in brief, and further review the evolution of WHO Classification of Tumors of CNS from the first edition to the fifth edition (WHO CNS5), including the category frame, tumor type/subtype and tumor grading. Changed from histology based classification into more biologically and molecularly defined entities, WHO CNS5 was recently released as expected, after attempt in WHO CNS4 updated version and 7 update publications upon the work of the Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy ⁃ Not Official WHO (cIMPACT ⁃ NOW). One of the important changes in WHO CNS5 is the separating gliomas into pediatric ⁃ type and adult ⁃ type based on their well ⁃ established molecular genetic differences. Tracing alone the clue in WHO CNS5, we introduce evolutionary process of entities and subtypes under each categories, and try to explain why they were added, deleted or why their naming was changed from WHO CNS1 to WHO CNS5. Tumor grading in WHO CNS5 has make efforts on moving close to how grading is done for non⁃CNS neoplasms, and added molecular parameters as biomarkers of grading based on histological features. WHO CNS5 has set new standers for clinical management of CNS tumors, and has been expected to facilitate related clinical studies and basic research in the future.

Waste concrete recycled powder (WCRP) occupies up to 30% of crushed old concrete and is hard to be reutilized due to its high porosity and low cementitious reactivity. Traditionally, the WCRP is reused as cement substitution in mortar/concrete production, but this substitution method has certain disadvantages. In this research, a novel substitution method, called the paste substitution method, was applied by replacing part of the cement paste with WCRP; a total of 28 trial mortar mixes containing WCRP added as either paste substitution or cement substitution were made for evaluation and analysis. By comparing the test results, it could be found that the incorporation of WCRP using the paste substitution method can remarkably enhance the compressive strength by at most 38.3%, densify the microstructure, and meanwhile greatly reduce the cement consumption by at most 26.7%. The cementing efficiency factor of WCRP in compressive strength was calculated for the first time, and its value is 0.289. Regression analysis showed that there exists good correlation between the compressive strength and effective water/cementitious materials (W/CM) ratio. [ABSTRACT FROM AUTHOR]

A novel noncontact indentation system with the combination of an air jet and optical coherence tomography (OCT) was presented in this paper for the quantitative measurement of the mechanical properties of soft tissues. The key idea of this method is to use a pressure-controlled air jet as an indenter to compress the soft tissue in a noncontact way and utilize the OCT signals to extract the deformation induced. This indentation system provides measurement and mapping of tissue elasticity for small specimens with high scanning speed. Experiments were performed on 27 silicone tissue-mimicking phantoms with different Young's moduli, which were also measured by uniaxial compression tests. The regression coefficient of the indentation force to the indentation depth (N mm(-1)) was used as an indicator of the stiffness of tissue under air jet indentation. Results showed that the stiffness coefficients measured by the current system correlated well with the corresponding Young's moduli obtained by conventional mechanical testing (r = 0.89, p < 0.001). Preliminary in vivo tests also showed that the change of soft tissue stiffness with and without the contraction of the underlying muscles in the hand could be differentiated by the current measurement. This system may have broad applications in tissue assessment and characterization where alterations of mechanical properties are involved, in particular with the potential of noncontact micro-indentation for tissues.

Byline: Shu-xin. Sun, Ji. Zhang, Ji-cheng. Sun, Zhi-jie. Chen, Xiang-heng. Zhang, Zheng-he. Chen, Jing-xiu. Huang, Yu. Jiang, Yong-gao. Mou, Zhong-ping. Chen, Ke. Sai Sir, A 51-year old man presented with [...]