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Abstract Subthalamic deep brain stimulation (STN DBS) has been a therapeutic choice for Parkinson’s disease (PD). We found that epidural motor cortex stimulation (MCS) with sustained positive (hyperpolarizing) currents could also consistently ameliorate the locomotor deficits in parkinsonian animals, rectifying the pathological paucity in both discharging unit varieties and movement-dependent spatiotemporal activity pattern changes in motor cortex (MC). Mechanistically, MCS hyperpolarizes both glutamatergic pyramidal neurons (PN) and GABAergic interneurons (IN) and consequently partly relieves PN from IN’s control. MC discharging units are thus enlarged with enhanced PN burst discharges against a relatively silenced background, presumably compensating for the hypoactive striatal selection to restore the MC activity changes upon movement. Behaviorally, MCS retains interim short pauses like normal locomotor behaviors, in contrast to the propensity of abnormal “restlessness” with STN DBS. Individually designed MCS, alone or in combination with STN DBS and dopaminergic therapy, may provide an optimal therapeutic approach for PD.

［Objective］ Currently, air-conditioning units in urban rail transit vehicles predominantly use an AC (alternating current) 380 V power supply mode, which requires two power conversions before reaching the end-use equipment, inevitably leading to some energy loss. Therefore, it is necessary to study the feasibility of applying DC (direct current)-powered air-conditioning units in urban rail transit vehicles. ［Method］ The operation mode of conventional AC 380V power supply is introduced, and a comparison is made between the circuit topologies of air-conditioning units under AC 380 V and DC 600 V power supply modes. The internal configurations of air-conditioning units in these two power supply modes are also compared. An energy-saving test is conducted using air-conditioning units of a rail transit vehicle in a Chinese city as example. Energy consumption indicators of the air-conditioning unit compressors under different frequencies for both power supply modes are tested, and a comparative analysis is performed on the energy consumption of conventional asynchronous motor fans and EC (electronically commutated) fans. ［Result & Conclusion］ Compared to AC 380 V-powered air-conditioning units, DC 600 V-powered units eliminate the two power conversion processes on the vehicle, achieving more significant energy saving effects. Additionally, DC 600 V-powered air-conditioning unit compressors demonstrate more pronounced energy-saving effects during low-frequency operation. Compared to conventional asynchronous motor fans, EC fans are more energy-efficient.

Abstract Background Chest trauma may precipitate a spectrum of injuries, among which tracheal laceration represents an uncommon but critical sequela. The occurrence of tracheal laceration could significantly impair respiratory and cardiovascular homeostasis, thereby posing a life-threatening risk to the patient. Expeditious surgical intervention is paramount for therapeutic management, and in certain instances, the administration of venovenous extracorporeal membrane oxygenation (VV ECMO) may be necessitated to support the patient’s respiratory function. Case presentation This case report discusses the surgical management of a delayed partial bronchial rupture following thoracic trauma, supported by VV ECMO. The patient, who sustained multiple rib fractures, hemopneumothorax, and scapular fractures due to a motor vehicle accident, developed progressive dyspnea one month post-trauma. Imaging revealed right lung atelectasis and pleural effusion, necessitating emergency surgery. VV ECMO was used to stabilize oxygenation, allowing for successful right main bronchus resection and anastomosis. Intraoperative bronchoscopy played a crucial role in identifying the rupture site and ensuring effective anastomosis. The patient recovered well, with no significant complications at follow-up. Conclusions This case highlights the importance of timely surgical intervention in bronchial rupture, the utility of VV ECMO for respiratory support, and the role of bronchoscopy in managing airway injuries. It underscores that delayed bronchial rupture, though rare, requires prompt recognition and treatment to prevent life-threatening complications.

In order to improve the adaptability of the quadruped robot to the complex road surface, a quadruped robot with three degrees of freedom on one leg was presented. Firstly, based on the virtual prototyping technology, the ratio of the lower leg was optimized, and the best ratio of the lower leg was obtained, which took into account both the speed and the motion stability. Based on this, the waist length of the robot was optimized by using the optimization feature of the genetic algorithm and taking the space utilization rate as the objective function. Then, the forward kinematics analysis was carried out based on the D-H method. Finally, the Monte Carlo estimation of the foot reachable space was carried out by using the Matlab software. The ideal standing height and step length of the robot were obtained, which laid a foundation for the subsequent gait planning of the robot.

Chronic nasal inflammation， such as rhinitis and sinusitis， is a common chronic disease that affects people’s quality of life for a long period of time. Its pathogenesis and mechanism are still unclear. The establishment of animal models is an effective approach to explore the pathogenesis， among which the establishment of mouse models of chronic nasal inflammation has been rapidly developed in recent years. The aim of this review is to summarize the research status of chronic rhinosinusitis and allergic rhinitis modeling in mice at home and abroad， especially the progress in eosinophilic chronic rhinosinusitis mouse models， which plays an important role in the development of new drugs to prevent and control chronic nasal inflammation or further elucidation of its pathogenesis.

To improve the physicochemical properties and processing performance of black wheat flour dough, the effects of adding Glucose oxidase (GOD) on the gelatinization, thermomechanics dough tensile, rheology properties, and microstructure of black wheat flour were investigated using a rapid viscosity analyzer, a texture analyzer, and a scanning electron microscope, among other methods. The effects were also explained in terms of changes in protein fractions. The results showed that the moderate addition of GOD could significantly reduce the content of free sulfhydryl groups and increase the content of disulfide bonds in the dough of black wheat flour, thereby enhancing the degree of protein cross-linking and significantly improving the physicochemical properties of black wheat flour dough. The best improvement in dough structure and properties was achieved when the GOD addition was 0.8%, at which point the ratio of wheat alcohol-soluble protein to wheat gluten (0.81) was close to the optimal ratio (1.0). This study has provided a certain reference for the development of black wheat-based foods and the improvement of their edible quality.

IntroductionB-cell lymphoma, a malignant proliferative disease originating from lymphoid tissue, poses a grave threat to human health. CD20 has emerged as a promising target for lymphoma treatment. However, due to the significant heterogeneity of B-cell lymphomas, conventional CD20 monoclonal antibodies show limited penetration, severely impeding the progress of B-cell lymphoma therapies.MethodsIn contrast, single-domain antibody molecules derived from cartilaginous fish have a molecular weight as small as 12 kDa, granting them robust penetration capabilities and making them the smallest known molecules of efficiently targeting specific antigens.ResultsAs a result, these molecules hold tremendous potential as candidate drugs for lymphoma treatment. In this study, the whitespotted bamboo shark (Chiloscyllium plagiosum) was immunized with recombinant human CD20 to generate specific single-domain antibodies (sdAbs) targeting CD20. By utilizing phage display technology, the variable new antigen receptors (VNARs) were successfully screened and identified, and play an important role in the inhibition of Raji lymphoblastoma.DiscussionThe sdAbs obtained through this research represent promising candidates for B-cell lymphoma treatment, displaying significant potential for clinical applications and offering a new direction for the development of targeted therapies against lymphoma.

Pancreatic cancer is a common malignant tumor in the digestive tract, and the difficulty of early diagnosis and the lack of effective treatment means are the main reasons for the poor prognosis of pancreatic cancer. In recent years, multidisciplinary treatment (MDT) has become an important means to break through the bottleneck of diagnosis and treatment of pancreatic cancer and improve clinical prognosis. Besides providing patients with high-quality diagnosis and treatment services, this treatment model helps to improve the clinical diagnosis and treatment level of specialists and cultivate high-quality compound medical talents. It also highlights clinical research groups and high-quality case resource sharing, and promotes the clinical application of innovative drugs and new diagnostic and therapeutic technologies, which plays an essential role in increasing the core competence of hospitals. This paper reviews and summarizes the origin, status quo, and deficiencies of the MDT diagnosis and treatment model of pancreatic cancer in China, and examines the prospects for future development, with the aim to provide reference for domestic and foreign counterparts.

Objective To construct SpyCatcher-mi3 nanoparticle vaccine delivery vectors, evaluate their role in enhancing the immunogenicity of the ovalbumin CD8+ T-cell epitope peptide, OVA257-264, and determine its protective effect in a model which mice were immunized and subcutaneously challenged with E.G7-OVA tumor cells. Methods SpyCatcher-mi3 proteins were expressed by E.coli and purified by affinity chromatography and anion exchange chromatography sequentially. OVA257-264-SpyTag peptide was obtained by synthesis. The OVA257-264-mi3 nanoparticles were produced by the SpyTag/SpyCatcher system. The toxicity of OVA257-264-mi3 was evaluated using hemolysis assay, CCK-8 assay and mouse experiment. A total of 42 female SPF-grade C57BL/6 mice (6~8 weeks old, 18~20 g) were randomly divided into OVA257-264-mi3, OVA257-264, and control groups, with 14 mice in each group. Then the mice in each group were immunized on days 0, 14 and 28. In 14 d after the last immunization, the amounts of spot-forming cells (SFCs, indicating IFN-γ secreting cells in splenic lymphocytes) were determined using ELISpot assay to evaluate their immunogenicity. After the immunized mice were subcutaneously implanted with E.G7-OVA tumor cells, the antitumor effect of the vaccine in prophylactic xenograft tumor model was evaluate by observing tumor volumes with a caliper and tumor growth with MRI. Results Both SpyCatcher-mi3 and OVA257-264-mi3 could be self-assembled to form homogeneous and stable nanoparticles, with an average particle size of about 43.8 and 91.3 nm, respectively. The OVA257-264-mi3 was safe for in vitro and in vivo toxicity evaluation. The number of IFN-γ secreting cells per 1×106 splenic lymphocytes reached 253 in the OVA257-264-mi3 group of mice, significantly higher than that in the OVA257-264 group and the Control group (P < 0.05). The tumor volume of mice in the OVA257-264-mi3 group was about 151.1 mm3 on day 22, which was significantly smaller than that of the OVA257-264 group and the Control group (P < 0.05), and the survival rate during the observation period reached 60%, which was significantly higher than that of the OVA257-264 groups (P < 0.05). Conclusion Nanoparticle vaccine OVA257-264-mi3 is successfully constructed, and it shows enhancing effect on the immunogenicity of the antigen epitope peptide, and exerts protective effect on prophylactic xenograft tumor model, providing a theoretical basis for the research of tumor neoantigen vaccines.

In the current preclinical anti-tumor researches, there is a general lack of an in vivo model that can quickly and efficiently screen effective anti-tumor drugs. As a species that is 87% genetically similar to humans, zebrafish have been widely used to model human diseases, and they are considered an alternative economic model for studying cancer development, proliferation, and metastasis. The zebrafish tumor xenograft model has been effectively used for cancer drug development at all levels, including target validation, and high-throughput screening of long non-coding RNAs (lncRNAs) that may be involved in tumor regulation. In this review, we provide a comprehensive overview of zebrafish as an in vivo model for cancer cell growth, migration, anti-tumor immunotherapy, and anti-tumor drug screening. In addition, the regulatory mechanisms of some active lncRNAs have been identified to play a role in the pathogenesis of cancer, but it is still necessary to take advantage of the efficient zebrafish model to screen and learn more about the role of these molecules in tumor development and migration. Current anti-tumor therapies are limited by severe toxicity and multidrug resistance. There is an urgent need for the cost-effective and efficient in vivo research tools to improve our understanding and overcome these problems. This paper reviews the different purposes of anti-tumor research using zebrafish model. We discuss the use of zebrafish in cancer cell proliferation and metastasis, identifying signaling pathways, cancer drug discovery and treatment development, and toxicity studies. Finally, this review highlights the limitations of the field and future directions to effectively utilize zebrafish as a highly efficient model for cancer treatment development.

Abstract Objectives Prolonged mechanical ventilation (PMV) is a common complication following cardiac surgery linked to unfavorable patient prognosis and increased mortality. This study aimed to search for the factors associated with the occurrence of PMV after valve surgery and to develop a risk prediction model. Methods The patient cohort was divided into two groups based on the presence or absence of PMV post-surgery. Comprehensive preoperative and intraoperative clinical data were collected. Univariate and multivariate logistic regression analyses were employed to identify risk factors contributing to the incidence of PMV. Based on the logistic regression results, a clinical nomogram was developed. Results The study included 550 patients who underwent valve surgery, among whom 62 (11.27%) developed PMV. Multivariate logistic regression analysis revealed that age (odds ratio [OR] = 1.082, 95% confidence interval [CI] = 1.042–1.125; P

Objective With the increasing demand for passenger comfort in urban rail transit, pressure wave control inside metro trains at speed of 120 km/h and above has become a an essential function of train air-conditioning system. Although high-speed EMUs and 160 km/h city railway trains already have mature pressure wave protection devices, most of them use pneumatic methods, complex in structure and relatively costly, therefore unsuitable for metro vehicles. Therefore, it is necessary to study pressure wave protection schemes suitable for metro vehicles based on their characteristics. Method Sampling tests are conducted on the pressure wave dynamics inside existing metro trains with speed of 120 km/h. Based on the test results and factors such as the operating routes and station layout, the primary influencing factors causing pressure wave dynamics inside the train are summarized, and a scheme using electric fresh air valves in the air conditioning system to control pressure wave dynamics inside the train is proposed. Validation of pressure waves inside the train is conducted under different opening degrees of the fresh air valve. Result & Conclusion Experimental results demonstrate that metro vehicles can utilize favorable factors such as fixed routes, fixed stations, and fixed dwell time to adopt an active pressure wave control scheme. The air-conditioning system controls the opening and closing of fresh air valves by using station information through the train network and speed signals, thereby controlling pressure wave dynamics inside the train. This scheme is relatively simple in structure, low in cost, and flexible in control compared to high-speed EMUs and 160 km/h city railway trains.

[Objective] Rainfall is the driving force of hydraulic erosion in black soil sloping farmland. It is of great significance to study soil erosion characteristics under natural rainfall conditions for soil erosion control. [Methods] Based on 43 erosive rainfall events observed at different ridge-row runoff plots (3°, 5° and 7°) along the slope in Guangrong Small Watershed, Hailun City, Heilongjiang Province during 2021—2022, K-Mean clustering method was used to compare the characteristics of runoff and sediment transport under different rain patterns and slopes, and the influencing factors on runoff and sediment transport were discussed. [Results] The rainfall of short duration, heavy rain and light rainfall (rain type 3) is the main one, followed by the rainfall of medium duration, medium rain intensity and medium rainfall (rain type 2), and the rainfall of long duration, light rain intensity and heavy rainfall (rain type 1) rarely occurs. Under the same rain pattern, the cumulative runoff depth and sediment transport increased with the increase of slope. Under the same slope, different rainfall types have different responses to cumulative runoff depth. Overall, rainfall type 3 has the highest contribution (50.90%～57.90%), rainfall type 2 has the second contribution (37.60%～44.10%), and rainfall type 1 has the lowest. In the event of rain type 2, rainfall erosivity and slope have direct influence on runoff depth, and runoff depth and slope have direct influence on sediment transport. In the event of rain type 3, rainfall erosivity affects runoff depth and then sediment transport through direct and indirect effects. [Conclusion] For all rainfall-type events, rainfall erosivity and slope are the main controlling factors affecting the runoff depth, and the runoff depth and slope are the main controlling factors affecting the sediment transport.

Accurate photovoltaic (PV) power forecasting is crucial for stable grid integration, particularly under rapidly changing weather conditions. This study presents an ultra-short-term forecasting model that integrates sky imager data and meteorological radar data, achieving significant improvements in forecasting accuracy. By dynamically tracking cloud movement and estimating cloud coverage, the model enhances performance under both clear and cloudy conditions. Over an 8-day evaluation period, the average forecasting accuracy improved from 67.26% to 77.47% (+15%), with MSE reduced by 39.2% (from 481.5 to 292.6), R2 increased from 0.724 to 0.855, NSE improved from 0.725 to 0.851, and Theil’s U reduced from 0.42 to 0.32. Notable improvements were observed during abrupt weather transitions, particularly on 1 July and 3 July, where the combination of MCR and sky imager data demonstrated superior adaptability. This integrated approach provides a robust foundation for advancing ultra-short-term PV power forecasting.

Objective This study investigated the effectiveness of remote administration of speech audiometry, an essential tool for diagnosing hearing loss and determining its severity. Utilizing two software tools for remote testing, the research aimed to compare these digital methods with traditional, in-person speech audiometry to evaluate their feasibility and accuracy. Design Participants underwent the Cantonese Hearing in Noise Test (CHINT) under three listening conditions—quiet, noise from the front, and noise from the right side—using three different administration methods: the conventional in-person approach, video conferencing software, and remote access software. Study Sample Fifty-six Cantonese-speaking adults residing in Hong Kong participated in this study. Results Analysis revealed no significant differences in CHINT scores among the three administration methods, indicating the potential for remote administration to yield results comparable to those of conventional methods. Conclusions The findings supported the feasibility of remote speech audiometry using the investigated digital tools. This study paved the way for the wider adoption of tele-audiology practices, particularly in situations where in-person assessments are not possible.

Abstract Background Most patients with intrahepatic cholangiocarcinoma (ICC) have developed distant metastasis at the time of diagnosis, while there is rear related nomogram to predict the prognosis. Methods Clinical data of patients pathologically diagnosed of ICC with distant metastasis were retrospectively collected from the Surveillance, Epidemiology, and End Results (SEER) database during 2005 to 2019. Finally, patients diagnosed as ICC in the Second Affiliated Hospital of Nanchang University from 2014 to 2019 were collected for external verification. All data were divided into training cohort and validation cohort in a ratio of 7:3. The nomogram was established based on independent prognostic factors using Cox univariate and multivariate analyses. The area under the receiver operating characteristic (ROC) curves (AUC), the calibration curve and the decision curve analysis (DCA) were used to determine the prediction accuracy of the nomogram. Results This study finally included 572 ICC with distant metastasis patients, another 32 patients collected by the author’s hospital were used as external verification. Results showed that age, surgery, radiotherapy and chemotherapy were independent prognostic factors, and nomogram was established. The AUC of predicting 3, 6, 9-month overall survival were 0.866, 0.841 and 0.786. The ROC curves and calibration curves showed that the nomogram had good predictive accuracy, and DCA showed that the nomogram had good clinical applicability. Conclusions The nomogram has good accuracy in predicting prognosis of DM-ICC patients, which would be of good significance to improve the prognosis of these patients.

Texture evolution during accumulative roll-bonding (ARB) is complicated because of the change in the through-thickness position that results from repeated cutting–stacking and roll-bonding. In this study, a macro–micro two-scale modeling was carried out to investigate the behaviors of texture evolution during ARB. The finite element method (FEM) was used to predict the strain history at a macro-scale, while a crystal plasticity FEM was used to reproduce the texture at a micro-scale. The texture evolution along three different cutting–stacking paths was traced and investigated. The patterns of texture transition between the rolling-type, shear-type, and random-type textures were studied by using area fractions of texture components, the distribution of textures, and the distribution of crystal rotation angles.

AIM: To analyze the effects of aromatase inhibitors(AIs)on the ocular surface microenvironment of the users.METHODS: A cross-sectional observational study was conducted. The study included postmenopausal women who received AIs treatment at galactophore department of our hospital from November 2022 to May 2023. Participants were divided into two groups based on the mechanism of AIs: the steroidal group and the non-steroidal group. The control group consisted of age-matched women who underwent occupational health examinations. All participants completed the ocular surface disease index(OSDI)questionnaire and underwent detailed ophthalmic examinations, including best-corrected visual acuity, intraocular pressure, axial length, corneal curvature, radius of curvature of curved lacrimal river surface, tear osmolarity, tear film break-up time, corneal fluorescein staining score, Schirmer Ⅰ test, and meibomian gland infrared score.RESULTS: There were no statistically significant differences in age, best-corrected visual acuity, intraocular pressure, axial length, and corneal curvature between control group and steroidal and non-steroidal group(P>0.05). The duration of drug treatment between the steroidal group and the non-steroidal group also showed no statistically significant difference(P>0.05). However, statistically significant differences were observed between the control group and the steroidal and non-steroidal group in OSDI scores, radius of curvature of curved lacrimal river surface, tear osmolarity, tear film break-up time, corneal fluorescein staining score, Schirmer Ⅰ test, and meibomian gland infrared score(P0.05).CONCLUSION: Postmenopausal patients receiving AIs treatment experienced significant changes in the ocular microenvironment, with both decreased tear secretion and excessive tear evaporation contributing to the occurrence of dry eye. Notably, patients receiving non-steroidal AIs treatment showed a more significant reduction in main lacrimal gland secretion.

The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb therapy, which requires effective blood perfusion and skeletal muscle regeneration. Herein, based on microfluidic technology, an integrated stem cell and cytokine co-delivery system designed for functional ischemic limb salvage was constructed by first incorporating the myogenic cytokine, fibroblast growth factor 19 (FGF19), into microspheres composed of methacrylate gelatin (GelMA). Then adipose-derived stem cells (ADSCs) were highly absorbed into the porous structure of the microspheres, overcoming the insufficient loading efficiency and activities by conventional encapsulation strategy. The fabricated ADSCs/FGF19@μsphere system demonstrated a uniform size of about 180 μm and a highly porous structure with pore sizes between 20 and 40 μm. The resultant system allowed high doses of ADSCs to be precisely engrafted in the lesion and to survive, and achieved sustained FGF19 release in the ischemic region to facilitate myoblast recruitment and differentiation and myofibrils growth. Furthermore, the combination of ADSCs and FGF19 exhibited a positive synergistic effect which substantially improved the therapeutic benefit of angiogenesis and myogenesis, both in vitro and in vivo. In summary, a stem cell and cytokine co-delivery system with the properties of easy preparation and minimal invasiveness was designed to ensure highly efficient cell delivery, sustained cytokine release, and ultimately realizes effective treatment of ischemic limb regeneration.

Porcine epidemic diarrhea virus (PEDV) is an acute, highly contagious enterovirus that infects pigs of all ages. The B cells are important for antigen presentation, antibody production, and cytokine secretion to resist infection. However, the role of B cells in PEDV infection remains unclear. In this study, the effects of PEDV virulent (QY2016) and attenuated strains (CV777) on B cells sorted from neonatal piglets, nursery piglets, and gilts were investigated. The results showed that PEDV-QY2016 and PEDV-CV777 could significantly increase the expression of CD54 and CD27 in B cells from neonatal piglets. The percentages of CD80, MHC II, and IgM expressed on neonatal piglet B cells infected with PEDV-QY2016 were significantly lower than those expressed on the B cells infected with PEDV-CV777. Both PEDV-QY2016 and PEDV-CV777 could stimulate IFN-α and GM-CSF secretions in neonatal piglet B cells; IL-1, IFN-α, and IL-4 secretion in nursery piglet B cells; and IL-1, TGF-β secretion, and GM-CSF in gilt B cells. Furthermore, both PEDV-QY2016 and PEDV-CV777 could induce the secretion of IgA, IgM, and IgG in nursery piglet B cells but could not induce the secretion of IgA, IgM, and IgG in neonatal piglet B cells. The secretion of IgA, IgM, and IgG was significantly higher by the PEDV-CV777 strains infected B cells than those by the PEDV-QY2016 strains infected gilt B cells. In conclusion, the surface molecule expression, cytokine secretion, and antibody production of B cells induced by PEDV are closely related to the ages of pigs and the virulence of the PEDV strain.

BackgroundMental health disorders in patients with multi-drug or rifampicin-resistant tuberculosis (MDR/RR-TB) receive consistent attention. Anxiety and depression can manifest and may impact disease progression in patients with MDR/RR-TB. Given the heightened stressors resulting from the COVID-19 pandemic, this scenario is even more concerning.ObjectiveTo evaluate the prevalence of and risk factors associated with anxiety and depression among patients with MDR/RR-TB in southern China.MethodsA facility-based cross-sectional study was undertaken at Guangzhou Chest Hospital in southern China, encompassing a cohort of 219 patients undergoing outpatient and inpatient treatment for MDR/RR-TB. Anxiety and depressive symptoms were assessed using the 7-Item Generalized Anxiety Disorder (GAD-7) scale and Patient Health Questionnaire-9 (PHQ-9). The ramifications of anxiety and depression were examined using univariate and multivariate logistic regression analyses, with odds ratios (ORs) and age- and sex-adjusted ORs (AORs) employed to quantify their influence. All data underwent statistical analysis using SPSS 25.0, with statistical significance established at P < 0.05.ResultsTwo hundred and nineteen individuals with MDR/RR-TB were included in the study. The prevalence of anxiety and depression was 57.53% (n = 126) and 65.75% (n = 144), respectively, with 33.3% (n = 73) of the participants experiencing both conditions simultaneously. Multivariate logistic regression analysis revealed that an age of 20–40 years [anxiety AOR = 3.021, 95% confidence interval (CI): 1.240–7.360; depression AOR = 3.538, 95% CI: 1.219–10.268], disease stigma (anxiety AOR = 10.613, 95% CI: 2.966–37.975; depression AOR = 4.514, 95% CI: 2.051–10.108) and poor physical health (anxiety AOR = 7.636, 95% CI: 2.938–19.844; depression AOR = 6.190, 95% CI: 2.468–15.529) were significant risk factors for moderate levels of anxiety and depression.ConclusionsWe found that individuals with MDR/RR-TB had an elevated risk of anxiety and depression. To decrease the likelihood of unfavorable treatment outcomes, it is imperative to carefully monitor the psychological wellbeing of patients with MDR/RR-TB and promptly address any detrimental psychiatric conditions.

Abstract Patients with pancreatic cancer commonly develop weight loss and muscle wasting. Whether adipose tissue and skeletal muscle losses begin before diagnosis and the potential utility of such losses for earlier cancer detection are not well understood. We quantify skeletal muscle and adipose tissue areas from computed tomography (CT) imaging obtained 2 months to 5 years before cancer diagnosis in 714 pancreatic cancer cases and 1748 matched controls. Adipose tissue loss is identified up to 6 months, and skeletal muscle wasting is identified up to 18 months before the clinical diagnosis of pancreatic cancer and is not present in the matched control population. Tissue losses are of similar magnitude in cases diagnosed with localized compared with metastatic disease and are not correlated with at-diagnosis circulating levels of CA19-9. Skeletal muscle wasting occurs in the 1–2 years before pancreatic cancer diagnosis and may signal an upcoming diagnosis of pancreatic cancer.

Abstract Background C-type lectin domain family 1 member B (CLEC1B, encoding the CLEC-2 protein), a member of the C-type lectin superfamily, is a type II transmembrane receptor involved in platelet activation, angiogenesis, and immune and inflammatory responses. However, data regarding its function and clinical prognostic value in hepatocellular carcinoma (HCC) remain scarce. Methods The expression of CLEC1B was explored using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. RT-qPCR, western blot, and immunohistochemistry assays were employed to validate the downregulation of CLEC1B. Univariate Cox regression and survival analyses were used to evaluate the prognostic value of CLEC1B. Gene Set Enrichment Analysis (GSEA) was conducted to investigate the potential association between cancer hallmarks and CLEC1B expression. The TISIDB database was applied to search for the correlation between immune cell infiltration levels and CLEC1B expression. The association between CLEC1B and immunomodulators was conducted by Spearman correlation analysis based on the Sangerbox platform. Annexin V-FITC/PI apoptosis kit was used for the detection of cell apoptosis. Results The expression of CLEC1B was low in various tumors and exhibited a promising clinical prognostic value for HCC patients. The expression level of CLEC1B was tightly associated with the infiltration of various immune cells in the HCC tumor microenvironment (TME) and positively correlated with a bulk of immunomodulators. In addition, CLEC1B and its related genes or interacting proteins are implicated in multiple immune-related processes and signaling pathways. Moreover, overexpression of CLEC1B significantly influenced the treatment effects of sorafenib on HCC cells. Conclusions Our results reveal that CLEC1B could serve as a potential prognostic biomarker and may be a novel immunoregulator for HCC. However, its function in immune regulation should be further explored.

Bladder cancer (BC) is the tenth most commonly diagnosed cancer. High recurrence, chemoresistance, and low response rate hinder the effective treatment of BC. Hence, a novel therapeutic strategy in the clinical management of BC is urgently needed. Medicarpin (MED), an isoflavone from Dalbergia odorifera, can promote bone mass gain and kill tumor cells, but its anti-BC effect remains obscure. This study reve aled that MED effectively inhibited the proliferation and arrested the cell cycle at the G1 phase of BC cell lines T24 and EJ-1 in vitro. In addition, MED could significantly suppress the tumor growth of BC cells in vivo. Mechanically, MED induced cell apoptosis by upregulating pro-apoptotic proteins BAK1, Bcl2-L-11, and caspase-3. Our data suggest that MED suppresses BC cell growth in vitro and in vivo via regulating mitochondria-mediated intrinsic apoptotic pathways, which can serve as a promising candidate for BC therapy.

Photovoltaic (PV) power generation has attracted widespread interest as a clean and sustainable energy source, with increasing global attention given to renewable energy. However, the operation and monitoring of PV power generation systems often result in large amounts of data containing missing values, outliers, and noise, posing challenges for data analysis and application. Therefore, PV data cleaning plays a crucial role in ensuring data quality, enhancing data availability and reliability. This study proposes a PV data cleaning method based on Rasterized Data Image Processing (RDIP) technology, which integrates rasterization and image processing techniques to select optimal contours and extract essential data. To validate the effectiveness of our method, we conducted comparative experiments using three data cleaning methods, including our RDIP algorithm, the Pearson correlation coefficient interpolation method, and cubic spline interpolation method. Subsequently, the cleaned datasets from these methods were utilized for power prediction using two linear regression models and two neural network models. The experimental results demonstrated that data cleaned using the RDIP algorithm improved the short-term forecast accuracy by approximately 1.0% and 3.7%, respectively, compared to the other two methods, indicating the feasibility and effectiveness of the RDIP approach. However, it is worth noting that the RDIP technique has limitations due to its reliance on integer parameters for grid division, potentially leading to coarse grid divisions. Future research efforts could focus on optimizing the selection of binarization thresholds to achieve better cleaning results and exploring other potential applications of RDIP in PV data analysis.

ContextAlthough the role of insulin-like growth factor I (IGF-1) in nonalcoholic fatty liver disease (NAFLD) has garnered attention in recent years, few studies have examined both reduced and elevated levels of IGF-1.ObjectiveThe aim of this study was to examine the potential relationship between IGF-1 levels and the risk of new-onset NAFLD in patients with pituitary neuroendocrine tumors (PitNET).MethodsWe employed multivariable Cox regression models and two-piecewise regression models to assess the association between IGF-1 and new-onset NAFLD. Hazard ratios (HRs) and their corresponding 95% confidence intervals (CIs) were calculated to quantify this association. Furthermore, a dose-response correlation between lgIGF-1 and the development of NAFLD was plotted. Additionally, we also performed subgroup analysis and a series sensitivity analysis.ResultsA total of 3,291 PitNET patients were enrolled in the present study, and the median duration of follow-up was 65 months. Patients with either reduced or elevated levels of IGF-1 at baseline were found to be at a higher risk of NAFLD compared to PitNET patients with normal IGF-1(log-rank test, P < 0.001). In the adjusted Cox regression analysis model (model IV), compared with participants with normal IGF-1, the HRs of those with elevated and reduced IGF-1 were 2.33 (95% CI 1.75, 3.11) and 2.2 (95% CI 1.78, 2.7). Furthermore, in non-adjusted or adjusted models, our study revealed a U-shaped relationship between lgIGF-1 and the risk of NAFLD. Moreover, the results from subgroup and sensitivity analyses were consistent with the main results.ConclusionsThere was a U-shaped trend between IGF-1 and new-onset NAFLD in patients with PitNET. Further evaluation of our discoveries is warranted.

Abstract Patients with pancreatic ductal adenocarcinoma (PDAC) commonly develop symptoms and signs in the 1–2 years before diagnosis that can result in changes to medications. We investigate recent medication changes and PDAC diagnosis in Nurses’ Health Study (NHS; females) and Health Professionals Follow-up Study (HPFS; males), including up to 148,973 U.S. participants followed for 2,994,057 person-years and 991 incident PDAC cases. Here we show recent initiation of antidiabetic (NHS) or anticoagulant (NHS, HFS) medications and cessation of antihypertensive medications (NHS, HPFS) are associated with pancreatic cancer diagnosis in the next 2 years. Two-year PDAC risk increases as number of relevant medication changes increases (P-trend

Abstract The nasal mucosa is constantly exposed to inhaled pathogens and is the first defence against respiratory infections. Here, we investigated the structural and compositional characteristics of the nasal mucosa of commercial pigs at various growth stages. The epithelial thickness, number of capillaries, and secretion function of the nasal mucosa dramatically increased with age; however, underlying lymphoid follicles in the respiratory region were rarely observed across the growth stages. The nasal mucosa was explored at the epithelial, immunological, and biological (commensal microbiota) barriers. In the epithelial barrier, the proliferative capacity of the nasal epithelia and the expression of tight junction proteins were high after birth; however, they decreased significantly during the suckling stage and increased again during the weaning stage. In the immunological barrier, most pattern recognition receptors were expressed at very low levels in neonatal piglets, and the innate immune cell distribution was lower. During the suckling stage, increased expression of Toll-like receptor (TLR) 2 and TLR4 was observed; however, TLR3 expression decreased. TLR expression and innate immune cell quantity significantly increased from the weaning to the finishing stage. In the biological barrier, Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes comprised the dominant phyla in neonatal piglets. A dramatic decrease in nasal microbial diversity was observed during the suckling stage, accompanied by an increase in potentially pathogenic bacteria. Proteobacteria, Bacteroidetes, and Firmicutes were identified as the core phyla of the nasal microbiota; among these, the three dominant genera, Actinobacter, Moraxella, and Bergerella, may be opportunistic pathogens in the respiratory tract. These characteristics comprise an essential reference for respiratory infection prevention at large-scale pig farms.

Porcine epidemic diarrhea virus (PEDV) causes acute watery diarrhea and high mortality in newborn piglets. Activation of intestinal mucosal immunity is crucial to anti-PEDV infection. To develop a vaccine capable of stimulating intestinal mucosal immunity, we prepared a bacterium (Lactococcus lactis)-like particle (BLP) vaccine (S1-BLPs) displaying the S1 protein, a domain of PEDV spike protein (S), based on gram-positive enhancer matrix (GEM) particle display technology. We further compared the effects of different vaccination routes on mucosal immune responses in mice induced by S1-BLPs. The specific IgG titer in serum of intramuscularly immunized mice with S1-BLPs was significantly higher than that of the intranasally administered. The specific IgA antibody was found in the serum and intestinal lavage fluid of mice vaccinated intranasally, but not intramuscularly. Moreover, the intranasally inoculated S1-BLPs induced higher levels of IFN-γ and IL-4 in serum than the intramuscularly inoculated. In addition, the ratio of serum IgG2a/IgG1 of mice inoculated intramuscularly was significantly higher with S1-BLPs compared to that of with S1 protein, suggesting that the immune responses induced by S1-BLPs was characterized by helper T (Th) cell type 1 immunity. The results indicated that S1-BLPs induced systemic and local immunity, and the immunization routes significantly affected the specific antibody classes and Th immune response types. The intranasally administered S1-BLPs could effectively stimulate intestinal mucosal specific secretory IgA response. S1-BLPs have the potential to be developed as PEDV mucosal vaccine.

Objective: This trial was to examine the effect of transcutaneous electrical acupoint stimulation (TEAS) on postoperative cognitive function in older patients who underwent thoracoscopic pulmonary resection. Methods: This was a prospective, randomized, double-blind, placebo-controlled study. 128 patients scheduled for surgery were randomly assigned to the TEAS group and sham-TEAS group. A standardized intervention of TEAS or sham-TEAS on the acupoints of Baihui (DU20) and bilateral Neiguan (PC6), Hegu (LI4), and Zusanli (ST36) from 30 min before anesthesia induction until the end of the surgery, combined with a general anesthetic protocol performed in the two groups respectively. The primary outcome was the incidence of postoperative cognitive dysfunction (POCD) assessed via the Montreal Cognitive Assessment (MoCA) scale at each time point. The secondary outcomes included the results of the Mini-Mental State Examination (MMSE) score, the Numerical Rating Scale (NRS) on pain and sleep, the European Organization for Research and Treatment of Cancer Quality of Life (EORTC-QLQ-C30), and a chronic pain questionnaire at relative time points. Results: Participants who completed the 12-month trial of the two groups were well-matched in baseline demographic and clinical parameters. At postoperative day 1, day 7, and day 30 time points, the incidence of POCD in the sham-TEAS group was always significantly higher than in the TEAS group (65.4% vs 20%, 43.6% vs 7.3%, 40% vs 3.6%, all P

Objective Cervical spondylotic myelopathy (CSM) is an incomplete spinal cord injury characterized with pain and stiffness in the neck and motor and sensory dysfunction. This study aims to determine whether C7–T1 intervertebral foramen area could be used as a parameter to evaluate the sagittal curvature of cervical spine. Methods Patients with clinical manifestations of spinal cord compression were hospitalized in our hospital from September 2018 to August 2019. All patients were diagnosed with CSM by nuclear magnetic imaging and other imaging methods. C2–C7 Cobb angle and T1 slop (T1S) were measured on the sagittal, T2‐weighted magnetic resonance image of cervical spine, and C7–T1 intervertebral foramen area were measured using oblique cervical spine X‐rays. Patients were divided into two groups according to the value of C2–C7 Cobb angle, including lordosis group (C2–C7 Cobb angle >10°, n = 45) and straight group (C2–7 Cobb angle ≤10°, n = 55). The reliability of the data was evaluated by intraclass correlation coefficient (ICC), and the correlation of the imaging parameters was analyzed by Pearson correlation. Results A total of 100 patients diagnosed with CSM hospitalized in our department were included. The ICC of the cervical parameters was 0.73. C7–T1 intervertebral foramen area was 40.69 ± 11.44 and 39.95 ± 10.94 mm2 in lordosis and straight group, respectively. The results showed that C7–T1 intervertebral foramen area was positively correlated with both C2–C7 Cobb angle (r = 0.23, p = 0.02) and T1S (r = 0.21, p = 0.03). In lordosis group, there was a positive correlation between C7 and T1 intervertebral foramen area and C2–C7 Cobb angle (r = 0.69, p

Abstract Background CDGSH iron sulfur domain 2 (CISD2) is an iron–sulfur protein with a [2Fe–2S] cluster, which is critical for cell proliferation and iron homeostasis. It has been demonstrated that aberrant expression of CISD2 is associated with the progression of multiple cancers. However, the underlying mechanism of CISD2 in regulating tumorigenesis remains obscure. Methods Bioinformatics strategies were used to investigate the protein interaction network and functional annotation of CISD2. In the functional experiment, cell viability was measured by CCK-8 kit. The levels of cellular reactive oxygen species (ROS), intracellular free iron, lipid peroxides, and lysosomal activity were determined by DCF-DA, RPA, C11-BODIPY, and cathepsin B staining, respectively. The glutathione (GSH) content was determined using a GSH assay kit. Results We showed that knockdown of CISD2 significantly accelerated the Erastin-induced ferroptotic cell death with excess lipid peroxidation, GSH exhaustion, and iron accumulation, while overexpression of CISD2 hindered the sensitivity to Erastin. Further assays via confocal microscopy and western blot exhibited that CISD2 knockdown markedly enhanced the lysosomal activity, and activated ferritinophagy under the exposure of Erastin. Pharmacological inhibition of lysosomal function could inhibit the degradation of ferritin heavy chain (FTH), and attenuate the phenotypes of ferroptosis, such as accelerated iron accumulation and lipid peroxidation. Notably, we found that Erastin-induced compensatory elevation of nuclear factor erythroid 2-related factor 2 (NRF2) could be eliminated in CISD2 depletion cells. Mechanically, CISD2 knockdown promoted the degradation of autophagy adaptor p62 and resulted in an increased binding affinity of Keap1 with NRF2, thus leading to the increased ubiquitination and subsequent degradation of NRF2. Enforced expression of NRF2 reversed the sensitivity of shCISD2 cells to ferroptosis both in vitro and in vivo. Conversely, enforced expression of Keap1 exacerbated the degradation of NRF2, reduced the transcriptional expression of FTH and heme oxygenase 1 (HO-1), increased the oxidative damage, and thus further facilitated ferroptosis. Conclusion Taken together, our current results illustrated two parallel mechanisms involved in the shCISD2-mediated ferroptosis. One was that shCISD2 enhanced the accumulation of free iron via ferritinophagy-dependent ferritin turnover; the other was that CISD2 depletion induced the inhibition of the p62–Keap1–NRF2 pathway, which resulted in oxidative stress and ferroptosis.

Objective: To study the effects of microbial nitrification inhibitors on the formation and changes of flavors in air-dried sausages. Methods: Four groups of air-dried sausages were designed: MNI group: 0.05% of microbial nitrosation inhibitors （MNI） were added; MNIP group: 0.05% MNI and an inoculation of PRO-MIX5 commercial starter （Staphylococcus xylose, Lactobacillus sake, and L. plantarum） were added; FBFAP group: fermented beef flavorings and compound antioxidants were added, and the sausages were inoculated with the PRO-MIX5 commercial starter; and CK group: blank control （CK）. The flavor of air-dried sausages was determined by an amino acid analyzer, Heracles iii ultra-fast gas phase electronic nose, electronic tongue and gas chromatography-mass spectrometry （GC-MS）. Results: Glutamate, alanine, taurine and carnosine are the primary free amino acids in the four groups of air-dried sausages. The addition of MNIP promoted the production of fresh amino acids and increased the total amount of free amino acids. Glutamate and alanine, two umami and sweet amino acids, contributed the most to the taste of air-dried sausages, and the four groups of air-dried sausages were primarily umami and sweet. There were differences in flavor and taste among the four groups. The electronic nose and electronic tongue principal component analysis （PCA） could distinguish between them. The flavors and taste of the MNI and MNIP groups were similar, and the taste of FBFAP group and CK were similar. The addition of MNI and MNIP improved the umami of air-dried sausage and reduced its saltiness and bitterness. Ethyl hexanoate and ethyl isovalerate were the characteristic flavors of the MNIP group. Linalool was the characteristic flavor of the MNI group, while decanal and ethyl isovalerate were the characteristic flavors of the FBFAP group. Conclusion: The addition of MNI alone or the synergistic effect of MNI and PRO-MIX5 promotes the formation of flavor in the air-dried sausages, indicating that MNI shows great promise at promoting the formation of flavor in air-dried sausages.

The analysis and utilization of potential information in data stream is an important part of data stream mining.Concept drift is a huge challenge for data stream mining that the distribution of data will change with time.Detecting changes in data distribution is a direct and effective method to detect concept drift.Currently,some concept drift detection methods use the tree structure or grid to establish a histogram to describe the data distribution.However,the tree structure is easy to produce inspection blind spots and leads to poor interpretability.While using the grid method on multi-dimensional data,the memory consumption is too much.To solve the above problems,a concept drift detection method for multi-dimensional data streams called partition based on uniform density clusters(PUDC) is proposed.The algorithm is based on the k-Means algorithm to partition the data with uniform density and uses the chi-square test for statistics and calculation of each partition to detect the concept drift.To ve-rify the validity of the method,four artificial datasets and three real datasets were selected for experiments.The type I and type II error rates of different dimensions of data were compared and analyzed.Experimental results show that PUDC algorithm is superior to several new algorithms in concept drift detection of multi-dimensional data streams.

Dianthus superbus L. has been extensively studied for its potential medicinal properties in traditional Chinese medicine and is often consumed as a tea by traditional folk. It has the potential to be exploited in the treatment of inflammation, immunological disorders, and diabetic nephropathy. Based on previous studies, this study continued the separation of another subfraction of Dianthus superbus and established reversed-phase/reversed-phase and reversed-phase/hydrophilic (RPLC) two-dimensional (2D) high-performance liquid chromatography (HPLC) modes, quickly separating two C-glycosylflavones, among which 2″-O-rhamnosyllutonarin was a new compound and isomer with 6‴-O-rhamnosyllutonarin. This is the first study to investigate the effects of 2″-O-rhamnosyllutonarin and 6‴-O-rhamnosyllutonarin on cellular glucose metabolism in vitro. First, molecular docking was used to examine the effects of 2″-O-rhamnosyllutonarin and 6″-O-rhamnosyllutonarin on AKT and AMPK; these two compounds exhibited relatively high activity. Following this, based on the HepG2 cell model of insulin resistance, it was proved that both of the 2″-O-rhamnosyllutonarin and 6‴-O-rhamnosyllutonarin demonstrated substantial efficacy in ameliorating insulin resistance and were found to be non-toxic. Simultaneously, it is expected that the methods developed in this study will provide a basis for future studies concerning the separation and pharmacological effects of C-glycosyl flavonoids.

Abstract Blockade of programmed cell death 1 ligand (PD-L1) has been used to treat triple-negative breast cancer (TNBC), and various strategies are under investigation to improve the treatment response rate. Inhibition of glutamine metabolism can reduce the massive consumption of glutamine by tumor cells and meet the demand for glutamine by lymphocytes in tumors, thereby improving the anti-tumor effect on the PD-L1 blockade therapy. Here, molybdenum disulfide (MoS2) was employed to simultaneously deliver anti-PDL1 antibody (aPDL1) and V9302 to boost the anti-tumor immune response in TNBC cells. The characterization results show that MoS2 has a dispersed lamellar structure with a size of about 181 nm and a size of 232 nm after poly (l-lysine) (PLL) modification, with high stability and biocompatibility. The loading capacity of aPDL1 and V9302 are 3.84% and 24.76%, respectively. V9302 loaded MoS2 (MoS2-V9302) can effectively kill 4T1 cells and significantly reduce glutamine uptake of tumor cells. It slightly increases CD8+ cells in the tumor and promotes CD8+ cells from the tumor edge into the tumor core. In vivo studies demonstrate that the combination of aPDL1 and V9302 (MoS2-aPDL1-V9302) can strongly inhibit the growth of TNBC 4T1 tumors. Interestingly, after the treatment of MoS2-aPDL1-V9302, glutamine levels in tumor interstitial fluid increased. Subsequently, subtypes of cytotoxic T cells (CD8+) in the tumors were analyzed according to two markers of T cell activation, CD69, and CD25, and the results reveal a marked increase in the proportion of activated T cells. The levels of cytokines in the corresponding tumor interstitial fluid are also significantly increased. Additionally, during the treatment, the body weights of the mice remain stable, the main indicators of liver and kidney function in the blood do not increase significantly, and there are no obvious lesions in the main organs, indicating low systemic toxicity. In conclusion, our study provides new insights into glutamine metabolism in the tumor microenvironment affects immune checkpoint blockade therapy in TNBC, and highlights the potential clinical implications of combining glutamine metabolism inhibition with immune checkpoint blockade in the treatment of TNBC. Graphical Abstract

The long - term stress damage caused by working load and additional load seriously affects the safe operation of the pipeline. Aimed at the stress detection of buried gas pipelines, the research was carried out on the pipeline weak magnetic stress external detection technology. By establishing the equivalent magnetic dipole model of the pipeline stress concentration zone, the numerical simulation of the normal component By and the tangential component Bx was carried out using Matlab software, and the distribution characteristics of the magnetic field components in stress concentration zone were obtained. A weak magnetic stress external detection on the Nanqi line 2 km gas pipeline under Changqing Oilfield combined with the magnetic field distribution characteristics of the stress concentration area obtained from the above - mentioned simulation analysis, 6 medium - risk stress damages and 14 low - risk stress damages were detected. The contact metal magnetic memory (MMM) detector was used to conduct excavation detection on two selected feature points. The MMM detection results of the two excavated pits were consistent with the pipeline weak magnetic stress external detection results. Results showed that the pipeline weak magnetic stress external detection technology was a new technology that could effectively detect pipeline stress damages, predict pipeline hazards and thus played an important role in assisting the safety management of pipelines.

Stimulated by the extraordinary power conversion efficiency (PCE) of hybrid organic-inorganic perovskites (HOIP) based solar cells (SCs), the derivative studies on inorganic perovskites (IOP) based SCs have been intensely investigated. In order to overcome the disadvantages of CsPbBr3, most prominently the unfavorable larger band gap (2.3eV), a grating layer of mesoporous anatase TiO2(mp-TiO2) has been inserted into the conventional configuration of SCs. The grating layer acts as the electron transfer layer (ETL) and light absorption strengthening layer at the same time. Due to the combined effects, the increased contacting area increased the fill factor (FF) and enhanced light trapping in the grating layer increased the short-current density, the average PCE of IOP based SCs has increased from 5.67% to 7.58%, which is a ca. 34% increase relatively. Furthermore, research on traditional HOIP-based SCs is also conducted. Interestingly, the increasing PCE mechanism is quite different from their inorganic counterparts, which should be attributed to the strain effect of different film structures. Thus, the strain-induced defect charge-state transitions of MAPbI3 by the grating layer increased the open-circuit voltage (Voc); and similarly, the increased contacting area also increased the FF, resulting in a 13% increase for PCE.

We demonstrate a simple, effective and feasible method to address the shrinkage of Poly (lactic-co-glycolic acid) (PLGA) through a core-shell structure fiber strategy. The results revealed that introducing size-stable poly-caprolactone (PCL) as the core fiber significantly improved the PLGA-based fibrous scaffold's dimensional maintenance. We further utilized fish collagen to modify the PLGA shell layer (PFC) of coaxial fibers and loaded baicalin (BA) into the PCL core layer (PCL-BA) to endow fibrous scaffold with more functional biological cues. The PFC/PCL-BA fibrous scaffold promoted the osteogenic differentiation of bone mesenchymal stem cells and stimulated the RAW264.7 cells to polarize into a pro-reparative phenotype. Importantly, the in vivo study demonstrated that the PFC/PCL-BA scaffold could regulate inflammation and osteoclast differentiation, favor neovascularization and bone formation. This work tactfully combined PLGA and PCL to establish a drug release platform based on the core-shell fibrous scaffold for vascularized bone regeneration.