18 results on '"Chendong Wu"'
Search Results
2. Dectin-1 aggravates neutrophil inflammation through caspase-11/4-mediated macrophage pyroptosis in asthma
- Author
-
Runjin Cai, Xiaoxiao Gong, Xiaozhao Li, Yuanyuan Jiang, Shuanglinzi Deng, Jiale Tang, Huan Ge, Chendong Wu, Huan Tang, Guo Wang, Lei Xie, Xuemei Chen, Xinyue Hu, and Juntao Feng
- Subjects
Asthma ,Dectin-1 ,Caspase-11 ,Pyroptosis ,Neutrophil ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background The pattern recognition receptor Dectin-1 was initially discovered to play a pivotal role in mediating pulmonary antifungal immunity and promoting neutrophil-driven inflammation. Recent studies have revealed that Dectin-1 is overexpressed in asthma, but the specific mechanism remains elusive. Additionally, Dectin-1 has been implicated in promoting pyroptosis, a hallmark of severe asthma airway inflammation. Nevertheless, the involvement of the non-classical pyroptosis signal caspase-11/4 and its upstream regulatory mechanisms in asthma has not been completely explored. Methods House dust mite (HDM)-induced mice was treated with Dectin-1 agonist Curdlan, Dectin-1 inhibitor Laminarin, and caspase-11 inhibitor wedelolactone separately. Subsequently, inflammatory cells in bronchoalveolar lavage fluid (BALF) were analyzed. Western blotting was performed to measure the protein expression of caspase-11 and gasdermin D (GSDMD). Cell pyroptosis and the expression of chemokine were detected in vitro. The correlation between Dectin-1 expression, pyroptosis factors and neutrophils in the induced sputum of asthma patients was analyzed. Results Curdlan appeared to exacerbate neutrophil airway inflammation in asthmatic mice, whereas wedelolactone effectively alleviated airway inflammation aggravated by Curdlan. Moreover, Curdlan enhanced the release of caspase-11 activation fragments and N-terminal fragments of gasdermin D (GSDMD-N) stimulated by HDM both in vivo or in vitro. In mouse alveolar macrophages (MH-S cells), Curdlan/HDM stimulation resulted in vacuolar degeneration and elevated lactate dehydrogenase (LDH) release. In addition, there was an upregulation of neutrophil chemokines CXCL1, CXCL3, CXCL5 and their receptor CXCR2, which was suppressed by wedelolactone. In asthma patients, a positive correlation was observed between the expression of Dectin-1 on macrophages and caspase-4 (the human homology of caspase-11), and the proportion of neutrophils in induced sputum. Conclusion Dectin-1 activation in asthma induced caspase-11/4 mediated macrophage pyroptosis, which subsequently stimulated the secretion of chemokines, leading to the exacerbation of airway neutrophil inflammation. Graphical Abstract
- Published
- 2024
- Full Text
- View/download PDF
3. Involvement of ERK and Oxidative Stress in Airway Exposure to Cadmium Chloride Aggravates Airway Inflammation in Ovalbumin-Induced Asthmatic Mice
- Author
-
Chendong Wu, Xinyue Hu, Yuanyuan Jiang, Jiale Tang, Huan Ge, Shuanglinzi Deng, Xiaozhao Li, and Juntao Feng
- Subjects
asthma ,cadmium ,ERK ,oxidative stress ,α-tocopherol ,Chemical technology ,TP1-1185 - Abstract
Inhalation represents a significant route of cadmium (Cd) exposure, which is associated with an elevated risk of lung diseases. This research study aims to evaluate the impact of repeated low-dose cadmium inhalation on exacerbating airway inflammation induced by ovalbumin (OVA) in asthma-afflicted mice. Mice were grouped into four categories: control (Ctrl), OVA, cadmium chloride (CdCl2), and OVA + cadmium chloride (OVA + CdCl2). Mice in the OVA group displayed increased airway mucus secretion and peribronchial and airway inflammation characterized by eosinophil cell infiltration, along with elevated levels of Th2 cytokines (IL-4, IL-5, IL-13) in bronchoalveolar lavage fluids (BALFs). These parameters were further exacerbated in the OVA + CdCl2 group. Additionally, the OVA + CdCl2 group exhibited higher levels of the oxidative stress marker malondialdehyde (MDA), greater activity of glutathione peroxidase (GSH-Px), and higher phosphorylation of extracellular regulated kinase (ERK) in lung tissue. Treatment with U0126 (an ERK inhibitor) and α-tocopherol (an antioxidant) in the OVA + CdCl2 group resulted in reduced peribronchial and airway inflammation as well as decreased airway mucus secretion. These findings indicate that CdCl2 exacerbates airway inflammation in OVA-induced allergic asthma mice following airway exposure. ERK and oxidative stress are integral to this process, and the inhibition of these pathways significantly alleviates the adverse effects of CdCl2 on asthma exacerbation.
- Published
- 2024
- Full Text
- View/download PDF
4. Complement regulatory proteins: Candidate biomarkers in differentiating tuberculosis pleural effusion
- Author
-
Huan Tang, Xinyue Hu, Li Li, Shuanglinzi Deng, Yuanyuan Jiang, Lisha Luo, Runjin Cai, Yifei Yang, Chendong Wu, Xiaoxiao Gong, and Juntao Feng
- Subjects
complement ,sCD46 ,sCD55 ,sCD59 ,tuberculosis pleural effusion ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background and aimsComplement activation is essential for tuberculosis pleural effusion. However, little is known about the value of complement regulatory protein (CD46, CD55, and CD59) in the differential diagnosis of tuberculosis.Materials and methodsNinety-nine patients with exudative pleural effusion admitted to Xiangya Hospital of Central South University from June 1, 2021to November 14, 2022 were enrolled. The expression levels of soluble CD46 (sCD46), soluble CD55 (sCD55), and soluble CD59 (sCD59) in pleural effusion were quantified by enzyme-linked immunosorbent assay, and the receiver operating characteristic (ROC) curves were plotted to evaluate the diagnostic and co-diagnostic values.ResultsThe ADA level is higher in TPE patients than non-TPE patients. It is well-found that TPE patients had lower levels of sCD46, sCD55, and sCD59 compared with non-TPE patients. Moreover, the expression of sCD46, sCD55, and sCD59 in pleural effusion was negatively correlated with ADA. In addition, the diagnostic efficacy of sCD46, sCD55 and sCD59 was comparable to that of ADA, with 0.896, 0.857, 0.858 and 0.893, respectively. Furthermore, combine detection of sCD46, sCD55, sCD59 and ADA could improve the diagnostic accuracy.ConclusionsComplement regulatory factors (CD46, CD55, and CD59) were validated by this project to be promising candidate biomarkers for the diagnosis of TPE with high accuracy. The combination of the CD46, CD55, and CD59 and ADA assay exist a better diagnostic value in TPE.
- Published
- 2023
- Full Text
- View/download PDF
5. Changes and Driving Mechanism of Microbial Community Structure during Paocai Fermentation
- Author
-
Pingmei Yan, Jingjing Jia, Huwei Zhao, and Chendong Wu
- Subjects
paocai ,microbial community ,high−throughput sequencing ,driving mechanism ,Fermentation industries. Beverages. Alcohol ,TP500-660 - Abstract
Fermentation of paocai is a dynamic process of the microbial community structure, and the interaction between community structure and physicochemical factors endows paocai with unique taste and flavor. The study of bacterial and fungal community structure changes and the driving mechanism of physicochemical factors induced changes in community structure, showing that Pseudomonas belonging to Proteobacteria and Lactobacillus belonging to Firmicutes were the dominant bacteria in the process of paocai fermentation. The correlation analysis of physicochemical factors with bacterial community showed that titratable acid was significantly positively correlated with Lactobacillus and negatively correlated with Pseudomonas, while nitrite was the opposite. Redundancy analysis (RDA) showed that pH was positively correlated with the bacterial community in the early fermentation stage, amino acid nitrogen was positively correlated with the bacterial community in the middle fermentation stage, and titratable acid was positively correlated with the bacterial community in the late fermentation stage. Variance partitioning analysis (VPA) showed that environmental factors, pH and metabolites, were the main driving forces of bacterial community diversity, which jointly explained 32.02% of the bacterial community structure variation. To study the glucolysis and nitrogen metabolism in the process of paocai fermentation, we found that in the early stage of the fermentation, the nitrite reductase enzyme of Pseudomonas activity was high, with high nitrite content in the prophase, but by the end of fermentation, lactic acid bacteria rapidly increased, the content of L−lactic acid through the glycolysis pathway, making paocai fermentation environment become acidic, then Pseudomonas decreased. Ascomycota and Basidiomycota were the main phylum fungi in the fermentation process. RDA analysis showed that the fungal community was positively correlated with pH, nitrite, and soluble protein at the early fermentation stage, amino acid nitrogen was positively correlated with the fungal community at the middle fermentation stage, titratable acid and reducing sugar were positively correlated with the fungal community at the late fermentation stage. VPA analysis showed that metabolites were the main driving force of fungal community diversity and accounted for 45.58% of fungal community diversity. These results had a certain guiding significance for the production and preservation of naturally fermented paocai.
- Published
- 2022
- Full Text
- View/download PDF
6. Reducing PDK1/Akt Activity: An Effective Therapeutic Target in the Treatment of Alzheimer’s Disease
- Author
-
Shaobin Yang, Yaqin Du, Xiaoqian Zhao, Chendong Wu, and Peng Yu
- Subjects
Alzheimer’s disease ,amyloid-β ,Tau ,3-phosphoinositide dependent kinase 1 ,protein kinase B ,phosphoinositide 3-kinase ,Cytology ,QH573-671 - Abstract
Alzheimer’s disease (AD) is a common age-related neurodegenerative disease that leads to memory loss and cognitive function damage due to intracerebral neurofibrillary tangles (NFTs) and amyloid-β (Aβ) protein deposition. The phosphoinositide-dependent protein kinase (PDK1)/protein kinase B (Akt) signaling pathway plays a significant role in neuronal differentiation, synaptic plasticity, neuronal survival, and neurotransmission via the axon–dendrite axis. The phosphorylation of PDK1 and Akt rises in the brain, resulting in phosphorylation of the TNF-α-converting enzyme (TACE) at its cytoplasmic tail (the C-terminal end), changing its internalization as well as its trafficking. The current review aimed to explain the mechanisms of the PDK1/Akt/TACE signaling axis that exerts its modulatory effect on AD physiopathology. We provide an overview of the neuropathological features, genetics, Aβ aggregation, Tau protein hyperphosphorylation, neuroinflammation, and aging in the AD brain. Additionally, we summarized the phosphoinositide 3-kinase (PI3K)/PDK1/Akt pathway-related features and its molecular mechanism that is dependent on TACE in the pathogenesis of AD. This study reviewed the relationship between the PDK1/Akt signaling pathway and AD, and discussed the role of PDK1/Akt in resisting neuronal toxicity by suppressing TACE expression in the cell membrane. This work also provides a perspective for developing new therapeutics targeting PDK1/Akt and TACE for the treatment of AD.
- Published
- 2022
- Full Text
- View/download PDF
7. Genetic evidence for variability in the social mating system of the Midday Gerbil (Meriones meridianus)
- Author
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Peng Yu, Chendong Wu, Zixuan Kong, Ruidong Cao, and Peng Chen
- Abstract
Social mating systems (e.g., monogamy, polygamy, or polyandry) are relatively stable behavioral strategies developed by environmental in animals, but the genetic imprint of a particular mating system is often incongruent with the social mating system due to extrapair matings. However, the genetics of mating systems remain little understood in rodents. In this study, we investigated the genetic signature of the mating system of 141 (63 females, 78 males) field-captured Midday Gerbils (Meriones meridianus) -a rodent species commonly found in the Minqin Desert in China-through microsatellite site analyses of genetic structure and relatedness. Seven pairs of highly polymorphic microsatellite loci were selected and were highly polymorphic, the combined exclusion probability was greater than 0.99. The parent pair paternity test by Cervus 3.0 software show that, eleven mother-offspring and nine father-offspring relationships were identified in 2018, involving 26 individuals from 10 families. Similarly, 19 mother-offspring and 19 father-offspring relationships were identified in 2019, involving 48 individuals from 18 families. All three types of genetic mating structure were identified: monogamy (19 families), polyandry (4 families), and polygyny (5 families), providing evidence that the genetics underlying mating systems in this species are variable, can be incongruent with behavioral evidence for social mating systems, and could vary based on environmental cues, including degree of perceived or actual predation.
- Published
- 2023
8. A solution of mixed integer linear programming for obstacle-avoided pursuit problem.
- Author
-
Liying Yang 0002, Jianda Han, Chendong Wu, and Yiyong Nie
- Published
- 2008
- Full Text
- View/download PDF
9. Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?
- Author
-
Huan Ge, Feifei Yin, Yuanyuan Jiang, Lisha Luo, Shuanglinzi Deng, Yingyu Zhang, Daimo Zhang, Yifei Yang, Huan Tang, Runjin Cai, Xinyue Hu, Chendong Wu, Xiaozhao Li, and Juntao Feng
- Abstract
Background: Allergic bronchopulmonary aspergillus (ABPA) is a complex non-infectious pulmonary benign disease caused by immune response against to aspergillus. Carcinoembryonic antigen (CEA) is a tumor marker but also elevated in some benign diseases. Few studies on ABPA with elevated serum CEA levels have been reported. Objects: This study aims to comb the clinical characters of ABPA with elevated serum CEA. Methods: 20 patients diagnosed as ABPA were divided into two groups (CEA normal and CEA elevated) for retrospective analysis. The eosinophil count and ratio, IgE level in the pretherapy and post-treatment were compared. Serum samples of patients with ABPA (n = 20) and asthma (n = 20), healthy controls (n=20) were collected. Levels of cytokines (IL-4, IL-5, GM-CSF, IFN-γ) were analyzed by enzyme-linked immunosorbent assay. Results: We found that in ABPA patients with normal serum CEA levels, eosinophil counts and IgE levels decreased more obviously after treatment. Besides, we established higher serum levels of IL-4, IL-5, GM-CSF and IFN-γ in ABPA patients with elevated serum CEA levels.Conclusion: For the ABPA patients with elevated serum CEA levels, CEA may serve as a monitoring indicator for severity and treatment efficacy of ABPA.
- Published
- 2021
10. Mentoring functions and turnover intention: the mediating role of perceived organizational support
- Author
-
Lili Zhang, Joon Hyung Park, Chendong Wu, Alexander Newman, and Angus Hooke
- Subjects
Organizational Behavior and Human Resource Management ,ComputingMilieux_THECOMPUTINGPROFESSION ,business.industry ,Strategy and Management ,education ,05 social sciences ,050209 industrial relations ,Public relations ,ComputingMilieux_GENERAL ,Work (electrical) ,Management of Technology and Innovation ,0502 economics and business ,Industrial relations ,Turnover intention ,Business and International Management ,Psychology ,business ,Perceived organizational support ,Social psychology ,050203 business & management - Abstract
Despite an increasing number of studies investigating the effects of mentoring on employee work outcomes, limited attention has been placed on the relationship between mentoring functions and turnover intentions. In this study, we examined the relationship between mentoring functions and turnover intentions, and the mediating role of perceived organizational support (POS) on this relationship. Using data collected from 176 employees in three Chinese banks, we found that POS partially mediated the relationship between mentoring functions and employees' turnover intentions.
- Published
- 2015
11. Walking gait generation using linear inverted pendulum model for biped robot with heterogeneous legs
- Author
-
Shiguang Wen, Jincheng Li, Fei Wang, and Chendong Wu
- Subjects
Engineering ,Robot kinematics ,Inverse kinematics ,Stability criterion ,business.industry ,Servomechanism ,law.invention ,Inverted pendulum ,Computer Science::Robotics ,Cog ,law ,Control theory ,Trajectory ,Robot ,business ,Simulation - Abstract
As a general advanced plateform of performance test and quantitative estimation for prosthetic leg, biped robot with heterogeneous legs (BRHL) simulates the amputee-prosthesis coupling system. To generate the walking gait trajectories of artificial leg, linear inverted pendulum model combining with ZMP stability criterion was employed and the reference angle of joints was calculated by establishing and solving robot inverse kinematics. To eliminate the effect of servo mechanism hysteresis, preview control was used to optimize the CoG trajectories. Experiment results indicate the effectiveness of the proposed scheme.
- Published
- 2010
12. A Power-Aware Layering Optimization Scheme for Wireless Sensor Network
- Author
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Xiaozhe Wang, Chendong Wu, Peng Ji, and Yunzhou Zhang
- Subjects
Computer science ,Network packet ,business.industry ,Distributed computing ,Quality of service ,Node (networking) ,Energy consumption ,Key distribution in wireless sensor networks ,Sensor node ,business ,Wireless sensor network ,Computer network ,Data transmission ,Efficient energy use - Abstract
Different from the traditional layer protocols, layering optimization can improve QoS of the whole wireless sensor network (WSN). Therefore, a novel power-aware layering optimization scheme is proposed to balance network lifetime and communication reliability. In the scheme, a directed spanning tree routing algorithm is discussed to resolve unnecessary energy consumption during data transmission. Regarded as a key factor, transmission power of node should be controlled to avoid occurrence of communication contention in some hot sub-area. And a Lagrange dual function is designed and iterated in order to find the optimal transmission power of every sensor node. These cross-layer schemes can lead WSN to get energy efficiency and smooth communication mode. With the simulations, the proposed scheme is evaluated in comparison with other related algorithm from energy consumption of nodes to number of received packets. The results shows that the scheme proposed in this paper have much better performance than others.
- Published
- 2009
13. A solution of mixed integer linear programming for obstacle-avoided pursuit problem
- Author
-
Liying Yang, Jianda Han, Yiyong Nie, and Chendong Wu
- Subjects
Mathematical optimization ,Linear programming ,Position (vector) ,Branch and price ,Convergence (routing) ,Motion planning ,Optimal control ,Integer programming ,Mathematics ,Integer (computer science) - Abstract
In this paper the path planning for obstacle-avoided pursuit problem (OAP) is studied. The OAP models based on the mixed integer linear programming (MILP) is presented. In the OAP models, the dynamic equation of mass point with linear damping is taken as the state equation of vehiclepsilas motion. Integer variables are used to describe the relative position of vehicle and obstacles. ldquoExpansible Target Sizerdquo is proposed to describe the pursuit process for target step-by-step. ldquoPursuit Directionrdquo of vehicle is defined. The Isometric Plane Method selected integer variables is used to solve MILP pursuit problem. How to select the integer variables of inner point is also given. Finally, simulations are given to show the efficiency of the method.
- Published
- 2008
14. Modeling of wave energy absorption based on BP neural network for underwater vehicles
- Author
-
Ying Zhang, Zaili Dong, and Chendong Wu
- Subjects
Engineering ,Artificial neural network ,business.industry ,Pendulum ,Control engineering ,computer.software_genre ,Backpropagation ,Simulation software ,Vehicle dynamics ,Wind wave ,Electronic engineering ,Absorption (electromagnetic radiation) ,business ,computer ,Energy (signal processing) - Abstract
As one of the renewable energy, the absorption and utilization of wave energy is always an important research field home and abroad, and energy absorption efficiency is the key. A wave energy absorption system based on inertial pendulum is presented, and dynamics equations are established. Neural network with learning rate adaptation is adopted to model in time domain. The results obtained by simulation software and by BP neural network are compared, which validate the method used are correct and make basis on future research.
- Published
- 2008
15. Entrepreneurial Leadership and Innovativeness: The Mediating Role of Team Psychological Safety
- Author
-
Joon Hyung Park, Angus Hooke, Lida Hu, and Chendong Wu
- Subjects
Entrepreneurial leadership ,Applied psychology ,Psychological safety ,General Medicine ,Psychology - Abstract
This study investigated the mediating effect of team psychological safety on the relationship between entrepreneurial leadership and innovativeness. We developed a model to explain this relationshi...
- Published
- 2014
16. Walking gait generation using linear inverted pendulum model for biped robot with heterogeneous legs.
- Author
-
Fei Wang, Shiguang Wen, Jincheng Li, and Chendong Wu
- Published
- 2010
- Full Text
- View/download PDF
17. A Power-Aware Layering Optimization Scheme for Wireless Sensor Network.
- Author
-
Peng Ji, Chendong Wu, Yunzhou Zhang, and Xiaozhe Wang
- Published
- 2009
- Full Text
- View/download PDF
18. Entrepreneurial Leadership and Innovativeness: The Mediating Role of Team Psychological Safety.
- Author
-
Hu, Lida, Joon Hyung Park, Chendong Wu, and Hooke, Angus
- Abstract
This study investigated the mediating effect of team psychological safety on the relationship between entrepreneurial leadership and innovativeness. We developed a model to explain this relationship and tested it using data covering 54 supervisors and 257 subordinates from a range of organizations. The results of partial least square structural equation modeling analysis (PLS-SEM) showed that team psychological safety mediates the relationship between entrepreneurial leadership and team innovativeness. We discuss the implications of the findings and identify possible directions for future research. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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