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Background: We aimed to investigate the clinical application value of Abbott Alinity analyzer in syphilis-specific antibody testing.

Objective To investigate the chemoresistance of human triple-negative breast cancer(TNBC) MDA-MB-468 cells induced by adriamycin(ADM) and the underlying mechanism. Methods MDA-MB-468 cells were treated with ADM at different concentrations for 24h, then MTT assay was used to detect the inhibitory rate of cell growth and the sensitivity of MDA-MB-468 cells to ADM. Immunofluorescence staining was used to detect the expression level of ABCG2 and Western blot was used to test the expression levels of phosphorylated Stat3(p-Stat3), cMyc and ABCG2. Results The sensitivity of MDA-MB-468/ADM to ADM was remarkably reduced and the expression of drug resistance protein ABCG2 was increased obviously in MDA-MB-468/ADM cells. In addition, the expressions of p-Stat3, cMyc and ABCG2 were much higher in MDA-MB-468/ADM cells than those in MDA-MB-468 cells. Interestingly, when the phosphorylation of Stat3 was inhibited by WP1066, the expressions of cMyc and ABCG2 were downregulated; furthermore, the sensitivity of MDA-MB-468/ADM cells to ADM was increased (P < 0.05). Conclusion ADM could induce the chemoresistance of human TNBC MDA-MB-468 cells through Stat3-cMyc signal pathway.

l‐Isocorypalmine, an active alkaloid compound isolated from Rhizoma Corydalis yanhusuo, has been reported to possess biological activity for treating cocaine use disorder. A high‐performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry method was established for identification of the metabolites of l‐isocorypalmine in urine, plasma and feces samples of rats after a single intragastric gavage of l‐isocorypalmine at a dose of 15 mg/kg. As a result, a total of 21 metabolites (six phase І metabolites and fifteen phase II metabolites) were detected and tentatively identified by mass spectrometry and fragment ions from tandem mass spectrometry spectra. All metabolites were present in the urine samples, nine metabolites were found in the plasma samples and three metabolites were found in the feces samples. Results indicated that metabolic pathways of l‐isocorypalmine included oxidation, dehydrogenation, demethylation, sulfate conjugation, and glucuronide conjugation. In addition, glucuronidation was the major metabolic reaction. Results of this investigation could provide significant experimental basis for efficacy, safety and action mechanism of l‐isocorypalmine, which will be advantageous to new drug development for treating cocaine addiction. [ABSTRACT FROM AUTHOR]

The resilience of unmanned aerial vehicle (UAV) swarm is its joint capability to resist possible threat, adapt to disruptive events, and restore its intended performance under a specific time period. The quantitative assessment of the UAV swarm resilience requires a thorough understanding of its missions. In this paper, a mission-oriented framework is proposed to implement the resilience evaluation for the UAV swarm. Guided by the framework, the resilience evaluation for the UAV swarm performing joint reconnaissance mission is studied. A UAV swarm model is developed for joint reconnaissance mission based on complex networks and agent-based models. The following aspects of the UAV swarm are considered in the proposed model, namely, the mission orientation, UAV attributes, swarm topology, UAV cooperative strategy, UAV information exchange and fusion strategy, potential threats, recovery strategies, etc. Then, a novel performance metric is proposed to measure the mission capability of the UAV swarm performing joint reconnaissance mission. Results from the simulations show that, compared with existing studies, the proposed approach can provide more realistic and objective resilience evaluation for the mission-oriented UAV swarm. The above works can be used to support the decision making and the optimal design of the UAV swarm, given different missions.