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Objective To investigate the effect of lncRNA HEIH on proliferation and apoptosis of lung cancer cells and its mechanism. Methods Human lung epithelial cells BEAS-2B and lung cancer cell lines A549, A427, H1299 and TKB-1 were cultured, and the expression level of HEIH was detected by RT-qPCR. Si-HEIH and miR-98-5p mimics were transfected into A549 cells inorder to silence the expression of HEIH in A549 cells or over-expressing miR-98-5p. Then cell proliferation was detected by MTT assay, apoptosis was examined by flow cytometry, and the levels of CCND1, caspase-3, SHH, GLI-1, PTCH and SUFU proteins were measured by Western blot. The dual luciferase reporter gene assay verified the relationship between HEIH and miR-98-5p. Results Compared with the normal lung epithelial cells BEAS-2B, the levels of HEIH in lung cancer cell lines,A549, A427, H1299 and TKB-1 were significantly increased (P＜0.05). Among them,the level of HEIH in lung cancer celllines A549 was the highest. Therefore, A549 cells were selected as the research object in the subsequent experiments. Silencing HEIH expression or over-expression of miR-98-5p reduced the A value of A549 cells after 12, 48 or 72 hours(P＜0.05)(MTT assay), increased apoptosis rates (P＜0.05) and inhibited CCND1 protein expression(P＜0.05), and promoted caspase-3 protein expression (P＜0.05). Over-expression of miR-98-5p also inhibited the mRNA and protein expression of SHH and GLI-1 in A549 cells (P＜0.05), and promoted the mRNA and protein expression of PTCH and SUFU (P＜0.05). Over-expression of HEIH reversed the effect of over-expression of miR-98-5p on proliferation and apoptosis of A549 cells and mRNA and protein expression of SHH, GLI-1, PTCH and SUFU. Conclusions Silencing HEIH expression may inhibit the proliferation of lung cancer cells and promote apoptosis by targeting miR-98-5p through Hedgehog signaling pathway.

Abstract: Adult specimens of Panulirus penicillatus were collected from localities distributed in Japan: Hachijojima Island, Ryukyu Archipelago (Amamioshima, Okinawajima, Ishigakijima); Taiwan: Taitung; Indonesia: Java Sea and Gebe Island; French Polynesia: [...]

Based on the investigation of present hedgerows in the upper reaches of Yangtze River, this paper analyzed the soil physical properties at different positions of three kinds of hedgerows (arbor, shrub, and grass). Comparing with those between the hedgerows, the soil physical properties within the hedgerows improved significantly. The average values of soil porosity, moisture content, saturated conductivity, water stable aggregates content, anti-erodibility index, anti-scouribility index, and clay content within the arbor, grass, and shrub hedgerows increased by 18.8%, 30.1%, 12.9%, 139.3%, 108.3%, 95.9%, and 25.5%, and the soil bulk density and sand content averagely decreased by 17.3% and 9.6%, respectively. The soil properties within the three hedgerows differed significantly. The soil anti-scouribility index within arbor hedgerow was the highest; the soil porosity, moisture content, saturated conductivity, anti-scouribility index, water-stable aggregates content, and clay content within the shrub hedgerow were higher than those within the tree and grass hedgerows; while the soil bulk density within the shrub hedgerows was lower than that within the tree and grass hedgerows. Because of the differences in the affecting degree of hedgerow on the soil physical properties at different positions of the three hedgerow systems, the related parameters presented definite horizontal variation at steep lands within, before, and behind the hedgerows, and between the hedgerows. The coefficient of variation (CV) of soil moisture content, anti-erodibility index, saturated conductivity, and clay content of arbor hedgerows was bigger than that of shrub and grass hedgerows, while the CV of soil bulk density, porosity, water-stable aggregates content, and anti-scouribility index of shrub hedgerow was bigger than that of arbor and grass hedgerows. [ABSTRACT FROM AUTHOR]

To explore the mutation characteristic of growth hormone ( GH) gene in geese, all the exons and introns of the gene were amplified by 20 pairs of primers, and then single nucleotide polymorphisms (SNPs) were detected by single strand conformation polymorphism (SSCP) and subsequently confirmed by sequencing. There were six SNPs per 1000 nucleotides in exons compared to two SNPs per 1000 nucleotides in intron regions. The variant in exons contained only one non-synonymous mutation and three synonymous mutations. The results show that its sequence identity with chicken and duck were 77.54% and 92.38%, respectively, which may be concluded that the GH gene was highly conservative in phylogenesis, although there were differences between waterfowls and chicken in their evolution process. [ABSTRACT FROM AUTHOR]

Brilliant blue dyeing and water breakthrough curve were applied to study the number and distribution of macropores and their relations to the preferential flow in typical sub-tropic broadleaved forest soils of Simian Mountains. The radii of the macropores were mainly between 0.3 and 3.0 mm, with the macroporosities in the range of 6.3% to 10.5%, and the macropores were always distributed in aggregation with increasing soil depth. The number of the macropores in each radius interval of dye-stained areas was tenfold increase than that of blank areas. The number of the macropores with radius larger than 0.3 mm, especially larger than 1.5 mm, was the most important factor affecting the occurrence of preferential flow. Significant correlations were found between the number of macropores and the water steady effluent volume, with the highest correlation coefficients of 0.842 and 0.879 for the radii intervals of 0.7-1.5 mm and 1.5-3.0 mm, respectively. Macropore continuity in dye-stained areas was better than that in blank areas, especially in the radius interval of 1.5-3.0 mm, with the biggest difference of 78.31%. In dye-stained areas, the number of macropores decreased gradually with soil depth. The filler-like distribution of macropores formed an effective water pressure gradient, which resulted in the preferential transport of water. [ABSTRACT FROM AUTHOR]

Based on the investigation of present hedgerows in the upper reaches of Yangtze River, this paper analyzed the soil physical properties at different positions of three kinds of hedgerows (arbor, shrub, and grass). Comparing with those between the hedgerows, the soil physical properties within the hedgerows improved significantly. The average values of soil porosity, moisture content, saturated conductivity, water stable aggregates content, anti-erodibility index, anti-scouribility index, and clay content within the arbor, grass, and shrub hedgerows increased by 18.8%, 30.1%, 12.9%, 139.3%, 108.3%, 95.9%, and 25.5%, and the soil bulk density and sand content averagely decreased by 17.3% and 9.6%, respectively. The soil properties within the three hedgerows differed significantly. The soil anti-scouribility index within arbor hedgerow was the highest; the soil porosity, moisture content, saturated conductivity, anti-scouribility index, water-stable aggregates content, and clay content within the shrub hedgerow were higher than those within the tree and grass hedgerows; while the soil bulk density within the shrub hedgerows was lower than that within the tree and grass hedgerows. Because of the differences in the affecting degree of hedgerow on the soil physical properties at different positions of the three hedgerow systems, the related parameters presented definite horizontal variation at steep lands within, before, and behind the hedgerows, and between the hedgerows. The coefficient of variation (CV) of soil moisture content, anti-erodibility index, saturated conductivity, and clay content of arbor hedgerows was bigger than that of shrub and grass hedgerows, while the CV of soil bulk density, porosity, water-stable aggregates content, and anti-scouribility index of shrub hedgerow was bigger than that of arbor and grass hedgerows.

Brilliant blue dyeing and water breakthrough curve were applied to study the number and distribution of macropores and their relations to the preferential flow in typical sub-tropic broad-leaved forest soils of Simian Mountains. The radii of the macropores were mainly between 0. 3 and 3.0 mm, with the macroporosities in the range of 6.3% to 10.5%, and the macropores were always distributed in aggregation with increasing soil depth. The number of the macropores in each radius interval of dye-stained areas was tenfold increase than that of blank areas. The number of the macropores with radius larger than 0.3 mm, especially larger than 1.5 mm, was the most important factor affecting the occurrence of preferential flow. Significant correlations were found between the number of macropores and the water steady effluent volume, with the highest correlation coefficients of 0.842 and 0.879 for the radii intervals of 0.7-1.5 mm and 1.5-3.0 mm, respectively. Macro-pore continuity in dye-stained areas was better than that in blank areas, especially in the radius interval of 1.5-3.0 mm, with the biggest difference of 78.31%. In dye-stained areas, the number of macropores decreased gradually with soil depth. The filler-like distribution of macropores formed an effective water pressure gradient, which resulted in the preferential transport of water.