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4. Evaluation of Autof MS 1000 and Vitek MS MALDI-TOF MS System in Identification of Closely-Related Yeasts Causing Invasive Fungal Diseases

Author

Yi, Qiaolian, primary, Xiao, Meng, additional, Fan, Xin, additional, Zhang, Ge, additional, Yang, Yang, additional, Zhang, Jing-Jia, additional, Duan, Si-Meng, additional, Cheng, Jing-Wei, additional, Li, Ying, additional, Zhou, Meng-Lan, additional, Yu, Shu-Ying, additional, Huang, Jing-Jing, additional, Chen, Xin-Fei, additional, Hou, Xin, additional, Kong, Fanrong, additional, Kudinha, Timothy, additional, and Xu, Ying-Chun, additional

Published
2021
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5. The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China

Author

Cheng, Jing-Wei, primary, Liu, Chang, additional, Kudinha, Timothy, additional, Xiao, Meng, additional, Fan, Xin, additional, Yang, Chun-Xia, additional, Wei, Ming, additional, Liang, Guo-Wei, additional, Shao, Dong-Hua, additional, Xiong, Zhu-Jia, additional, Hou, Xin, additional, Yu, Shu-Ying, additional, Wang, Yao, additional, Yang, Qi-Wen, additional, Su, Jian-Rong, additional, and Xu, Ying-Chun, additional

Published
2020
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6. Distribution and Antifungal Susceptibility of Candida Species Causing Candidemia in China: An Update From the CHIF-NET Study

Author

Xiao, Meng, primary, Chen, Sharon C-A, primary, Kong, Fanrong, primary, Xu, Xiu-Li, primary, Yan, Li, primary, Kong, Hai-Shen, primary, Fan, Xin, primary, Hou, Xin, primary, Cheng, Jing-Wei, primary, Zhou, Meng-Lan, primary, Li, Ying, primary, Yu, Shu-Ying, primary, Huang, Jing-Jing, primary, Zhang, Ge, primary, Yang, Yang, primary, Zhang, Jing-Jia, primary, Duan, Si-Meng, primary, Kang, Wei, primary, Wang, He, primary, and Xu, Ying-Chun, primary

Published
2020
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8. Trichosporon dohaense, a rare pathogen of human invasive infections, and literature review

Author

Yu, Shu-Ying, Guo, Li-Na, Xiao, Meng, Kudinha, Timothy, Kong, Fanrong, Wang, He, Cheng, Jing-Wei, Zhou, Meng-Lan, Xu, Hui, and Xu, Ying-Chun

Subjects
Infection and Drug Resistance, reduced susceptibility, identification, invasive infection, emerging pathogen, Original Research, Trichosporon dohaense
Abstract

Shu-Ying Yu,1–3 Li-Na Guo,1,3 Meng Xiao,1,3 Timothy Kudinha,4,5 Fanrong Kong,4 He Wang,1,3 Jing-Wei Cheng,1–3 Meng-Lan Zhou,1–3 Hui Xu,6 Ying-Chun Xu1–3 1Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China; 2Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China; 3Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China; 4Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR – Pathology West, University of Sydney, Westmead Hospital, Westmead, NSW, Australia; 5Centre for Infectious Diseases and Microbiology Laboratory Services, Westmead Hospital, Westmead, NSW, Australia; 6Department of Clinical Laboratory, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China Background: Trichosporon dohaense is a rare fungal species that has not been described in human invasive infections. Patients and methods: In this study, we investigated two T. dohaense isolates from patients with invasive infections in two hospitals in China, as part of the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program. Both patients were under immunocompromised conditions. Results: On chromogenic agar, T. dohaense isolates were dark blue, similar to the color of Candida. tropicalis, but the characteristic moist colony appearance was quite different from that of T.asahii. The two isolates were misidentified as T. asahii and T. inkin by the VITEK 2 YST system. The rDNA internal transcribed spacer (ITS) region and the D1/D2 domain sequences of the two T.dohaense isolates were 100% identical to T. dohaense type strain CBS10761T. The sequence of the intergenic spacer region-1 also clearly distinguished the species. Of the three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems, Bruker Biotyper and Autobio MS correctly identified the two isolates to species level, whereas Vitek MS systems misidentified them as T. ovoides or T. asteroides. Echinocandins exhibited no in vitro activities against the two T. dohaense isolates. In addition, the isolates exhibited intermediate susceptibility to fluconazole (with minimal inhibitory concentrations [MICs] of 8 and 16 µg/mL) and itraconazole, voriconazole, and posaconazole (MICs of 0.25–1 µg/mL). T. dohaense demonstrated susceptibility to amphotericin B with MIC of 1 µg/mL. The MICs of fluconazole and voriconazole in our study were higher than the MIC50 of 62 for T. asahii isolates (4 and 0.064 µg/mL) in the CHIF-NET program. Conclusion: This case study points to a possible emergence of T. dohaense as an opportunistic human invasive fungal pathogen, and the reduced susceptibility should be noted. Keywords: Trichosporon dohaense, invasive infection, emerging pathogen, identification, reduced susceptibility

Published
2018

9. Molecular epidemiology of Clostridium difficile in two tertiary care hospitals in Shandong Province, China

Author

Luo,Ying, Zhang,Wen, Cheng,Jing-Wei, Xiao,Meng, Sun,Gui-Rong, Guo,Cheng-Jie, Liu,Ming-Jun, Cong,Pei-Shan, and Kudinha,Timothy

Subjects
Infection and Drug Resistance
Abstract

Ying Luo,1,2 Wen Zhang,2 Jing-Wei Cheng,3 Meng Xiao,3 Gui-Rong Sun,1 Cheng-Jie Guo,2 Ming-Jun Liu,1 Pei-Shan Cong,1 Timothy Kudinha4,5 1Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China; 2Department of Clinical Laboratory, Zibo Central Hospital, Zibo, China; 3Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China; 4Charles Sturt University, Orange, NSW, Australia; 5Central West Pathology Laboratory, Orange, NSW, Australia Purpose: The incidence and severity of Clostridium difficile infection (CDI) have markedly increased over the past decade. However, there is very limited epidemiological data on CDI in China so far, specifically no data in Shandong Province. The aim of this study was to evaluate diagnostic algorithm for CDI and to gain data on molecular epidemiology of CDI in the Shandong Province of China.Materials and methods: Nonrepetitive unformed fecal specimens (n=504) were investigated by the glutamate dehydrogenase (GDH), C. difficile toxin A&B (CDAB) tests and toxigenic culture. Furthermore, 85 isolates were characterized by toxin gene detection, multilocus sequence typing, ribotyping and antimicrobial susceptibility testing.Results: The algorithm of combining GDH and CDAB tests could define diagnosis of 54.2% CDI cases and excluded 90% of non-CDI. Further adding the toxigenic culture to the algorithm enhanced the detection sensitivity to 100%. Toxigenic strains comprised 84.7% of isolates, including A+B+CDT− (71.8%, 61/85), A–B+CDT– (11.8%, 10/85) and A+B+CDT+ (1.2%, 1/85) isolates. RT046/ST35 (13.9%, 10/72), RT014/ST2 (12.5%, 9/72) and RT017/ST37 (12.5%, 9/72) were the more common genotypes among toxigenic C. difficile strains. The clinical severity score of A–B+CDT– toxin genes genotype (3.50±0.85) was significantly higher than the A+B+CDT– type (2.59±0.93) (P

Published
2018

10. High in vitroactivity of fidaxomicin against Clostridium difficileisolates from a university teaching hospital in China

Author

Cheng, Jing-Wei, Yang, Qi-Wen, Xiao, Meng, Yu, Shu-Ying, Zhou, Meng-Lan, Kudinha, Timothy, Kong, Fanrong, Liao, Jian-Wei, and Xu, Ying-Chun

Abstract

Clostridium difficileinfection (CDI) is a significant cause of morbidity and mortality in both the acute care setting and the wider healthcare system. The purpose of this study was to evaluate the in vitroactivity of fidaxomicin against C.difficileisolates from a university teaching hospital in China.

Published
2024
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18. Use of next generation sequence to investigate potential novel macrolide resistance mechanisms in a population of Moraxella catarrhalis isolates

Author

Liu, Ya-Li, primary, Li, Dong-Fang, additional, Xu, He-Ping, additional, Xiao, Meng, additional, Cheng, Jing-Wei, additional, Zhang, Li, additional, Xu, Zhi-Peng, additional, Chen, Xin-Xin, additional, Zhang, Ge, additional, Kudinha, Timothy, additional, Kong, Fanrong, additional, Gong, Yan-Ping, additional, Wang, Xin-Ying, additional, Zhang, Yin-Xin, additional, Wu, Hong-Long, additional, and Xu, Ying-Chun, additional

Published
2016
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20. Five-year China Hospital Invasive Fungal Surveillance Net (CHIF-NET) study of invasive fungal infections caused by noncandidal yeasts: species distribution and azole susceptibility.

Author

Xiao, Meng, Chen, Sharon C–A, Kong, Fanrong, Fan, Xin, Cheng, Jing-Wei, Hou, Xin, Zhou, Meng-Lan, Wang, He, and Xu, Ying-Chun

Subjects
MYCOSES, AZOLES, MICROBIAL sensitivity tests, DESORPTION, FLUCONAZOLE
Abstract

Purpose: In this study, we report results from a 5-year surveillance for noncandidal yeast species causing invasive infections from 65 hospitals in China.Materials and methods: Species identification was carried out by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) supplemented by rDNA sequencing, and fluconazole and voriconazole susceptibilities of yeasts were determined by Clinical and Laboratory Standards Institute (CLSI) disk diffusion methods.Results: Overall, 884 noncandidal isolates belonging to 38 species were collected. Cryptococcus neoformans was the most common (75.6%), which also comprised 96.5% of the isolates from cerebrospinal fluid (CSF) and 62.6% from blood, followed by Trichosporon asahii (6.9%) and Rhodotorula mucilaginosa (5.1%). Fluconazole susceptibility and resistant rates were 74.1% and 9.7% for C. neoformans and 81.0% and 5.2% for T. asahii. Voriconazole exhibited good activity in comparison to these two species (99.5% and 98.3% of the isolates, were susceptible). However, 100% of the R. mucilaginosa isolates were resistant to both azoles. Other noncandidal yeast species showed reduced susceptibility to fluconazole (53.3%) but most were susceptible to voriconazole (94.3%). Over the 5 years, a decrease in the proportion of fluconazole-susceptible isolates was observed for C. neoformans (90%–67%, P [ABSTRACT FROM AUTHOR]

Published
2018
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22. Sequencer-Based Capillary Gel Electrophoresis (SCGE) Targeting the rDNA Internal Transcribed Spacer (ITS) Regions for Accurate Identification of Clinically Important Yeast Species

Author

Hou, Xin, primary, Xiao, Meng, additional, Chen, Sharon C.-A., additional, Wang, He, additional, Zhang, Li, additional, Fan, Xin, additional, Xu, Zhi-Peng, additional, Cheng, Jing-Wei, additional, Kong, Fanrong, additional, Zhao, Yu-Pei, additional, and Xu, Ying-Chun, additional

Published
2016
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23. Accurate Identification of Common Pathogenic Nocardia Species: Evaluation of a Multilocus Sequence Analysis Platform and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Author

Xiao, Meng, primary, Pang, Lu, additional, Chen, Sharon C-A., additional, Fan, Xin, additional, Zhang, Li, additional, Li, Hai-Xia, additional, Hou, Xin, additional, Cheng, Jing-Wei, additional, Kong, Fanrong, additional, Zhao, Yu-Pei, additional, and Xu, Ying-Chun, additional

Published
2016
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25. The Role of Glutamate Dehydrogenase (GDH) Testing Assay in the Diagnosis of Clostridium difficile Infections: A High Sensitive Screening Test and an Essential Step in the Proposed Laboratory Diagnosis Workflow for Developing Countries like China

Author

Cheng, Jing-Wei, primary, Xiao, Meng, additional, Kudinha, Timothy, additional, Xu, Zhi-Peng, additional, Sun, Lin-Ying, additional, Hou, Xin, additional, Zhang, Li, additional, Fan, Xin, additional, Kong, Fanrong, additional, and Xu, Ying-Chun, additional

Published
2015
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26. First case report of endocarditis caused by haematobacter massiliensis in China.

Author

Jing-wei Cheng, Peng Wang, Meng Xiao, Ying Yuan, Timothy Kudinha, Ying Zhao, Fanrong Kong, Ying-chun Xu, Cheng, Jing-Wei, Wang, Peng, Xiao, Meng, Yuan, Ying, Kudinha, Timothy, Zhao, Ying, Kong, Fanrong, and Xu, Ying-Chun

Subjects
ENDOCARDITIS, RNA sequencing, AMOXICILLIN, MICROBIOLOGY, ORGANISMS, LEFT heart ventricle, HEART physiology, DNA metabolism, CLAVULANIC acid, BACTERIA, COMBINATION drug therapy, DNA, INFECTIVE endocarditis, RNA, SEQUENCE analysis, DIAGNOSIS, THERAPEUTICS
Abstract

Background: Haematobacter massiliensis, a rare species of fastidious Gram-negative, non-motile, non-sporing, non-fermentative, pleomorphic, aerobic bacilli, has rarely been documented as the cause of infectious endocarditis in literature. Here we report the first case of infectious endocarditis (IE) caused by H. massiliensis in China.Case Presentation: A 44-year-old woman presented to the infectious department of Peking Union Medical College Hospital (Beijing) in August 2013, with a 7-week history of fevers, chills, sore throat, muscular soreness, occasional joint pain, and cough. The organism obtained by blood culture, identified as H. massiliensis by 16S rRNA gene sequencing, was finally implicated as the cause of infectious endocarditis. The patient was cured with amoxicillin/clavulanate combined with amikacin for 6 weeks.Conclusion: This is the first case report in China, of the isolation of H. massiliensis from the bloodstream of a patient with endocarditis. The microbiology and clinical study of the organism will help us understand it better in future clinical practice. [ABSTRACT FROM AUTHOR]

Published
2017
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34. Synthesis, Magnetic and Electromagnetic Absorption Properties of Amorphous Core-Shell Fe-B@SiO2 Submicrospheres

Author

Zhong, Xuan, Cheng, Jing Wei, Liu, Ying, and Zhao, Xiu Chen

Abstract

Amorphous core-shell Fe-B@SiO2 submicrospheres with the SiO2 shell layer of about 18 nm were fabricated via a two-step process. Fe-B submicrospheres were first obtained, and core-shell Fe-B@SiO2 submicrospheres were subsequently fabricated using TEOs as a Si source through a modified Stöber method. The measurements of the magnetic properties demonstrated that the amorphous core-shell Fe-B@SiO2 submicrospheres exhibit ferromagnetic behavior at room temperature. The maximum reflection loss reaches −24.8 dB at 11.76 GHz for the absorber with thickness of 2.2 mm. The absorption bandwidth with the reflection loss below −10 dB is up to 14.76 GHz for the absorber with a thickness of 1.5-6 mm and the absorption bandwidth with the reflection loss below −20 dB is up to 6.6 GHz for the absorber with a thickness of 1.8-3.1 mm. Our results demonstrates that the amorphous core-shell Fe-B@SiO2 submicrospheres obtained in this work are attractive candidate materials for the magnetic and EM wave absorption applications.

Published
2017
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36. Identification and Antifungal Susceptibility Profiles of Candida haemuloniiSpecies Complex Clinical Isolates from a Multicenter Study in China

Author

Hou, Xin, Xiao, Meng, Chen, Sharon C.-A., Wang, He, Cheng, Jing-Wei, Chen, Xin-Xin, Xu, Zhi-Peng, Fan, Xin, Kong, Fanrong, and Xu, Ying-Chun

Abstract

ABSTRACTCandida haemuloniicomplex (Candida haemulonii, Candida haemuloniivar. vulnera, and Candida duobushaemulonii) consists of emerging pathogens. Thirty-one isolates from 14 hospitals in China were studied for their species classification and antifungal susceptibilities. Performances of molecular (i.e., ribosomal DNA [rDNA] internal transcribed spacer [ITS] sequencing, D1/D2 sequencing, and ITS sequencer-based capillary gel electrophoresis [SCGE]) and phenotypic identification methods in species identification were compared. Twenty-six (83.9%) of 31 isolates were identified as C. haemuloniiand 5 isolates were identified as C. duobushaemuloniiby ITS sequencing as the reference method; results obtained by D1/D2 sequencing and ITS SCGE were concordant with those obtained by ITS sequencing for all (100%) of the isolates. All 31 isolates were identified as C. haemuloniiby the Vitek matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system (bioMérieux, France), whereas the Bruker MS system (Bruker Daltoniks, Germany) correctly provided species identification for 77.4% and 100% of isolates using cutoff scores for species of =2.0 and =1.70, respectively. The Vitek 2 compact (bioMérieux) only identified 9 (29%) of 31 isolates. All isolates showed high MICs for amphotericin B (range, 2 to >8 µg/ml) and fluconazole (=128 µg/ml) but low MICs (=0.5 µg/ml) for the echinocandins. Our results reinforce the need for MALDI-TOF MS and/or molecular differentiation of species within the C. haemuloniicomplex. The multiresistant antifungal susceptibility profile of these isolates represents a challenge to therapy.

Published
2016
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37. Identification and Antifungal Susceptibility Profile of Candida guilliermondiiand Candida fermentatifrom a Multicenter Study in China

Author

Cheng, Jing-Wei, Yu, Shu-Ying, Xiao, Meng, Wang, He, Kudinha, Timothy, Kong, Fanrong, and Xu, Ying-Chun

Abstract

ABSTRACTWith molecular sequencing as a gold standard, the Vitek MS, Bruker Biotyper MS, and Vitek-2 Compact systems correctly identified 92.7%, 97.0%, and 15.2% of 164 Candida guillermondiiisolates, respectively, and none of 8 C. fermentatiisolates. All of the isolates showed high susceptibility to echinocandins, but some C. guilliermondiiisolates showed low azole susceptibility.

Published
2016
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38. Five-Year National Surveillance of Invasive Candidiasis: Species Distribution and Azole Susceptibility from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study

Author

Xiao, Meng, Sun, Zi-Yong, Kang, Mei, Guo, Da-Wen, Liao, Kang, Chen, Sharon C.-A., Kong, Fanrong, Fan, Xin, Cheng, Jing-Wei, Hou, Xin, Zhou, Meng-Lan, Li, Ying, Yu, Shu-Ying, Huang, Jing-Jing, Wang, He, and Xu, Ying-Chun

Abstract

ABSTRACTData on the epidemiology of invasive candidiasis (IC) and the antifungal susceptibility of Candidaisolates in China are still limited. Here we report on surveillance for IC from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) study. Sixty-five tertiary hospitals collected 8,829 Candidaisolates from 1 August 2009 to 31 July 2014. Matrix-assisted laser desorption ionization–time of flight mass spectrometry supplemented by ribosomal DNA sequencing was used to define the species, and the fluconazole and voriconazole susceptibilities were determined by the Clinical and Laboratory Standards Institute disk diffusion method. A total of 32 Candidaspecies were identified. Candida albicanswas the most common species (44.9%), followed by the C. parapsilosiscomplex (20.0%), C. tropicalis(17.2%), and the C. glabratacomplex (10.8%), with other species comprising <3% of isolates. However, in candidemia, the proportion of cases caused by C. albicanswas only 32.3%. C. albicansand C. parapsilosiscomplex isolates were susceptible to fluconazole and voriconazole (<6% resistance), while fluconazole and azole cross-resistance rates were high in C. tropicalis(13.3% and 12.9%, respectively), C. glabratacomplex (18.7% and 14%, respectively), and uncommon Candidaspecies (44.1% and 10.3%, respectively) isolates. Moreover, from years 1 to 5 of the study, there was a significant increase in the rates of resistance to fluconazole among C. glabratacomplex isolates (12.2% to 24.0%) and to both fluconazole (5.7% to 21.0%) and voriconazole (5.7% to 21.4%) among C. tropicalisisolates (P< 0.01 for all comparisons). Geographic variations in the causative species and susceptibilities were noted. Our findings indicate that antifungal resistance has become noteworthy in China, and enhanced surveillance is warranted.

Published
2018
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