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809 results on '"Cheng, Seng"'

2. Adeno-associated viral vector serotype 9–based gene therapy for Niemann-Pick disease type A

Author

Samaranch, Lluis, Pérez-Cañamás, Azucena, Soto-Huelin, Beatriz, Sudhakar, Vivek, Jurado-Arjona, Jerónimo, Hadaczek, Piotr, Ávila, Jesús, Bringas, John R, Casas, Josefina, Chen, Haifeng, He, Xingxuan, Schuchman, Edward H, Cheng, Seng H, Forsayeth, John, Bankiewicz, Krystof S, and Ledesma, María Dolores

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Gene Therapy, Rare Diseases, Biotechnology, Brain Disorders, Neurosciences, Neurodegenerative, Genetics, Neurological, Animals, Brain, Dependovirus, Genetic Therapy, Humans, Inflammation, Injections, Liver, Mice, Knockout, Motor Activity, Niemann-Pick Disease, Type A, Primates, Serogroup, Sphingomyelin Phosphodiesterase, Transgenes, Biological Sciences, Medical and Health Sciences, Medical biotechnology, Biomedical engineering
Abstract

Niemann-Pick disease type A (NPD-A) is a lysosomal storage disorder characterized by neurodegeneration and early death. It is caused by loss-of-function mutations in the gene encoding for acid sphingomyelinase (ASM), which hydrolyzes sphingomyelin into ceramide. Here, we evaluated the safety of cerebellomedullary (CM) cistern injection of adeno-associated viral vector serotype 9 encoding human ASM (AAV9-hASM) in nonhuman primates (NHP). We also evaluated its therapeutic benefit in a mouse model of the disease (ASM-KO mice). We found that CM injection in NHP resulted in widespread transgene expression within brain and spinal cord cells without signs of toxicity. CM injection in the ASM-KO mouse model resulted in hASM expression in cerebrospinal fluid and in different brain areas without triggering an inflammatory response. In contrast, direct cerebellar injection of AAV9-hASM triggered immune response. We also identified a minimally effective therapeutic dose for CM injection of AAV9-hASM in mice. Two months after administration, the treatment prevented motor and memory impairment, sphingomyelin (SM) accumulation, lysosomal enlargement, and neuronal death in ASM-KO mice. ASM activity was also detected in plasma from AAV9-hASM CM-injected ASM-KO mice, along with reduced SM amount and decreased inflammation in the liver. Our results support CM injection for future AAV9-based clinical trials in NPD-A as well as other lysosomal storage brain disorders.

Published
2019

8. Molecular characterisation of nerol dehydrogenase gene (PmNeDH) from Persicaria minor in response to stress-related phytohormones

Author

Cheng Seng Tan, Nur-Athirah Abd-Hamid, Jin Kiat Chew, Maizom Hassan, Ismanizan Ismail, Chyan Leong Ng, and Zamri Zainal

Subjects
nerol dehydrogenase, defence-related gene, phytohormones, persicaria minor, Plant culture, SB1-1110, Plant ecology, QK900-989
Abstract

Citral is a mixture of neral and geranial, which are of great interest to the fragrance industry due to its lemon-scented aroma. A newly characterized nerol dehydrogenase of Persicaria minor (PmNeDH) from our recent findings has shown a capacity to convert citral from nerol. Differential gene expression analysis revealed that the expression level of PmNeDH was highly upregulated during early treatment of several stress-related phytohormones i.e. methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA). SA and ABA were shown to have a prolonged effect on PmNeDH expression level until second day of treatment. The findings were in agreement with the cis-regulatory elements predicted from the gene promoter. The phylogenetic relationship of PmNeDH with its homologs from the medium-chain dehydrogenases/reductases (MDR) superfamily was also mentioned. In this study, we proposed a possible biological function of PmNeDH gene in P. minor, which might play significant roles in plant defence mechanism.

Published
2019
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9. Relicensing Practices of Taxi Drivers and Crane Operators Aged 70 Years and above in Singapore

Author

Mei Leng Chan, Yoko Wong, Karen Tan, Joon Cheng Seng, Stephanie Jia Xian Ho, Claire Jing Wen Wong, and Gerald Choon-Huat Koh

Subjects
taxi driver, crane operator, licensing, evidence-based practice, assessment, ageing, Social Sciences
Abstract

Singapore is a rapidly ageing society, underpinned by national policies to promote successful and active ageing. Whilst older worker participation in the workforce is encouraged, policymakers are challenged to ensure that work competency is not compromised with any normal age- and/or health-related changes. This paper will briefly outline how policymakers responded to the needs of two subgroups of older workers aged 70 years and above (taxi drivers; crane operators) who desire to continue working in the last two decades. Whilst a mandatory retirement age policy exists for older taxi drivers in Singapore, there is none for older crane operators. Despite this, stricter relicensing protocols were introduced for both types of workers, with active collaboration involving additional occupational therapy services for functional work competency assessments to complement the routine medical fitness screening. Comparisons will be made of these two relicensing frameworks, including the mention of any relevant studies to align with the call of evidence-based practices. In mid-2020, the relicensing policy for older taxi drivers was revised based on findings of a retrospective national database study. Currently, a 4-year national database study on older crane operators aged 70 years and above is being undertaken with preliminary findings to be reported.

Published
2022
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10. Merits of Combination Cortical, Subcortical, and Cerebellar Injections for the Treatment of Niemann-Pick Disease Type A

Author

Bu, Jie, Ashe, Karen M, Bringas, John, Marshall, John, Dodge, James C, Cabrera-Salazar, Mario A, Forsayeth, John, Schuchman, Edward H, Bankiewicz, Krystof S, Cheng, Seng H, Shihabuddin, Lamya S, and Passini, Marco A

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Brain Disorders, Neurosciences, Animals, Brain, Dependovirus, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Genetic Therapy, Genetic Vectors, Injections, Macaca fascicularis, Male, Mice, Mice, Knockout, Niemann-Pick Disease, Type A, Primates, Sphingomyelin Phosphodiesterase, Biological Sciences, Technology, Medical and Health Sciences, Biotechnology, Genetics, Clinical sciences, Medical biotechnology
Abstract

Niemann-Pick disease Type A (NPA) is a neuronopathic lysosomal storage disease (LSD) caused by the loss of acid sphingomyelinase (ASM). The goals of the current study are to ascertain the levels of human ASM that are efficacious in ASM knockout (ASMKO) mice, and determine whether these levels can be attained in non-human primates (NHPs) using a multiple parenchymal injection strategy. Intracranial injections of different doses of AAV1-hASM in ASMKO mice demonstrated that only a small amount of enzyme (10 mg hASM/g tissue was reached. In monkeys, injection of 12 tracts of AAV1-hASM resulted in efficacious levels of enzyme in broad regions of the brain that was aided, in part, by axonal transport of adeno-associated virus (AAV) and movement through the perivascular space. This study demonstrates that a combination cortical, subcortical, and cerebellar injection protocol could provide therapeutic levels of hASM to regions of the NHP brain that are highly affected in NPA patients. The information from this study might help design new AAV-mediated enzyme replacement protocols for NPA and other neuronopathic LSDs in future clinical trials.

Published
2012

11. Fetal gene therapy for neurodegenerative disease of infants

Author

Massaro, Giulia, Mattar, Citra N. Z., Wong, Andrew M. S., Sirka, Ernestas, Buckley, Suzanne M. K., Herbert, Bronwen R., Karlsson, Stefan, Perocheau, Dany P., Burke, Derek, Heales, Simon, Richard-Londt, Angela, Brandner, Sebastian, Huebecker, Mylene, Priestman, David A., Platt, Frances M., Mills, Kevin, Biswas, Arijit, Cooper, Jonathan D., Chan, Jerry K. Y., Cheng, Seng H., Waddington, Simon N., and Rahim, Ahad A.

Published
2018
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12. 'Benign' temporal lobe epilepsy with hippocampal sclerosis: A forgotten entity?

Author

Emmanuel Cheng Seng Leong and Udaya Seneviratne

Subjects
Temporal lobe epilepsy, Hippocampal sclerosis, Late-onset, Benign, Neurology. Diseases of the nervous system, RC346-429, Neurophysiology and neuropsychology, QP351-495
Abstract

Mesial temporal lobe epilepsy, a well-characterized epilepsy syndrome, is most commonly accompanied by the pathological feature of hippocampal sclerosis. Patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) typically present in childhood to early adolescence. We describe a cohort of patients who presented with their first epileptic seizure in late adulthood with atypical features. We characterized five patients with late-onset MTLE-HS by describing their demographics, electroclinical features, imaging, autoantibody status, and response to antiseizure medication (ASM) therapy. All five patients had their first seizure after the age of 50 with no history of initial precipitating incidents. None of our patients had positive serum or CSF autoantibodies and they have all remained seizure-free for a minimum of two years on ASMs alone. Two patients had motor vehicle crashes associated with seizures whilst three patients experienced seizures in sleep. A milder form of MTLE, previously described as benign TLE, does exist in routine clinical practice. These patients respond well to ASM therapy, but potentially harmful consequences such as motor vehicle crashes can occur in the untreated.

Published
2020
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15. Microarray dataset of transgenic rice overexpressing Abp57

Author

Lay Wen Tan, Cheng Seng Tan, Zuraida A. Rahman, Hoe-Han Goh, Ismanizan Ismail, and Zamri Zainal

Subjects
Microarray, Abp57, Overexpressing, Rice, Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390
Abstract

The dataset presented in this article describes microarray experiment of Auxin-binding protein 57, Abp57-overexpressing transgenic rice. The gene expression profiles were generated using Affymetrix GeneChip® Rice (Cn) Gene 1.0 ST Arrays. Total RNA from seedlings tissue of transgenic rice and wildtype, which serve as control were used as starting materials for microarray experiment. Detailed experimental methods and data analysis were described here. The raw and normalized microarray data were deposited into Gene Expression Omnibus (GEO) under accession number GSE99055.

Published
2017
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20. Dysregulated DNA methylation in the pathogenesis of Fabry disease

Author

Shen, Jin-Song, primary, Balaji, Uthra, additional, Shigeyasu, Kunitoshi, additional, Okugawa, Yoshinaga, additional, Jabbarzadeh-Tabrizi, Siamak, additional, Day, Taniqua S., additional, Arning, Erland, additional, Marshall, John, additional, Cheng, Seng H., additional, Gu, Jinghua, additional, Schiffmann, Raphael, additional, Bottiglieri, Teodoro, additional, and Goel, Ajay, additional

Published
2022
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22. Agarwood Induction: Current Developments and Future Perspectives

Author

Cheng Seng Tan, Nurulhikma Md Isa, Ismanizan Ismail, and Zamri Zainal

Subjects
Aquilaria, agarwood, inducer, high-throughput omics, data integration, Plant culture, SB1-1110
Abstract

Agarwood is a resinous part of the non-timber Aquilaria tree, which is a highly valuable product for medicine and fragrance purposes. To protect the endangered Aquilaria species, mass plantation of Aquilaria trees has become a sustainable way in Asian countries to obtain the highly valuable agarwood. As only physiologically triggered Aquilaria tree can produce agarwood, effective induction methods are long sought in the agarwood industry. In this paper, we attempt to provide an overview for the past efforts toward the understanding of agarwood formation, the evolvement of induction methods and their further development prospects by integrating it with high-throughput omics approaches.

Published
2019
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26. The safety profile of a cationic lipid-mediated cystic fibrosis gene transfer agent following repeated monthly aerosol administration to sheep

Author

Alton, Eric W.F.W., Baker, Alison, Baker, Eilidh, Boyd, A. Christopher, Cheng, Seng H., Coles, Rebecca L., Collie, D. David S., Davidson, Heather, Davies, Jane C., Gill, Deborah R., Gordon, Catherine, Griesenbach, Uta, Higgins, Tracy, Hyde, Stephen C., Innes, J. Alastair, McCormick, Dominique, McGovern, Michael, McLachlan, Gerry, Porteous, David J., Pringle, Ian, Scheule, Ronald K., Shaw, Darren J., Smith, Sionagh, Sumner-Jones, Stephanie G., Tennant, Peter, and Vrettou, Christina

Published
2013
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36. Molecular characterization of OsCURT1A from upland rice in response to osmotic stress

Author

Nurulhikma Md Isa, Cheng Seng Tan, Bernadette Toni, Ismanizan Ismail, and Zamri Zainal

Subjects
0106 biological sciences, 0301 basic medicine, Genetics, biology, Mutant, Wild type, food and beverages, Heterologous, Plant Science, biology.organism_classification, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Arabidopsis, Chlorophyll, Coding region, Arabidopsis thaliana, Agronomy and Crop Science, Gene, 010606 plant biology & botany
Abstract

CURT1 proteins in 'Arabidopsis thaliana' have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from 'Oryza sativa' cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The 'OsCURT1A' gene shares 80% of its amino acid sequence with Arabidopsis 'AtCURT1A'. Phylogenetic analysis revealed that the 'OsCURT1A' is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::'OsCURT1A'), T-DNA insertional mutant line ('atcurt1a'), and Wild type (Col-0). However, overexpression of 'OsCURT1A' under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the 'OsCURT1A' might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, 'OsCURT1A' can be used as a potential candidate for salinity stress tolerance in crop improvement.

Published
2019

37. Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer

Author

Griesenbach, Uta, Vicente, Catarina C., Roberts, Megan J., Meng, Cuixiang, Soussi, Samia, Xenariou, Stefania, Tennant, Peter, Baker, Alison, Baker, Eilidh, Gordon, Catherine, Vrettou, Christina, McCormick, Dominique, Coles, Rebecca, Green, Anne-Marie, Lawton, Anna E., Sumner-Jones, Stephanie G., Cheng, Seng H., Scheule, Ronald K., Hyde, Stephen C., Gill, Deborah R., Collie, David D., McLachlan, Gerry, and Alton, Eric W.F.W.

Published
2011
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44. The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways

Author

Griesenbach, Uta, Meng, Cuixiang, Farley, Raymond, Wasowicz, Marguerite Y., Munkonge, Felix M., Chan, Mario, Stoneham, Charlotte, Sumner-Jones, Stephanie G., Pringle, Ian A., Gill, Deborah R., Hyde, Stephen C., Stevenson, Barbara, Holder, Emma, Ban, Hiroshi, Hasegawa, Mamoru, Cheng, Seng H., Scheule, Ronald K., Sinn, Patrick L., McCray, Paul B., Jr., and Alton, Eric W.F.W.

Published
2010
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50. Targeting nuclear RNA for in vivo correction of myotonic dystrophy

Author

Wheeler, Thurman M., Leger, Andrew J., Pandey, Sanjay K., MacLeod, A. Robert, Nakamori, Masayuki, Cheng, Seng H., Wentworth, Bruce M., Bennett, C. Frank, and Thornton, Charles A.

Subjects
RNA -- Physiological aspects -- Health aspects, Myotonic dystrophy -- Genetic aspects -- Physiological aspects -- Development and progression, Oligonucleotides -- Physiological aspects -- Health aspects -- Genetic aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Antisense oligonucleotides (ASOs) hold promise for gene-specific knockdown in diseases that involve RNA or protein gain-offunction effects. In the hereditary degenerative disease myotonic dystrophy type 1 (DM1), transcripts from the [...]

Published
2012

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