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8 results on '"Chengkun Xia"'

1. High-throughput CRISPR technology: a novel horizon for solid organ transplantation

Author

Xiaohan Li, Zhang Chen, Weicong Ye, Jizhang Yu, Xi Zhang, Yuan Li, Yuqing Niu, Shuan Ran, Song Wang, Zilong Luo, Jiulu Zhao, Yanglin Hao, Junjie Zong, Chengkun Xia, Jiahong Xia, and Jie Wu

Subjects
solid organ transplantation, CRISPR/Cas9, transcriptome editing, CRISPR screening, high-throughput, Immunologic diseases. Allergy, RC581-607
Abstract

Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.

Published
2024
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2. Interorgan communication with the liver: novel mechanisms and therapeutic targets

Author

Jiulu Zhao, Xi Zhang, Yuan Li, Jizhang Yu, Zhang Chen, Yuqing Niu, Shuan Ran, Song Wang, Weicong Ye, Zilong Luo, Xiaohan Li, Yanglin Hao, Junjie Zong, Chengkun Xia, Jiahong Xia, and Jie Wu

Subjects
liver, organ communication, hepatokine, immunity, immune homeostasis, immune cells, Immunologic diseases. Allergy, RC581-607
Abstract

The liver is a multifunctional organ that plays crucial roles in numerous physiological processes, such as production of bile and proteins for blood plasma, regulation of blood levels of amino acids, processing of hemoglobin, clearance of metabolic waste, maintenance of glucose, etc. Therefore, the liver is essential for the homeostasis of organisms. With the development of research on the liver, there is growing concern about its effect on immune cells of innate and adaptive immunity. For example, the liver regulates the proliferation, differentiation, and effector functions of immune cells through various secreted proteins (also known as “hepatokines”). As a result, the liver is identified as an important regulator of the immune system. Furthermore, many diseases resulting from immune disorders are thought to be related to the dysfunction of the liver, including systemic lupus erythematosus, multiple sclerosis, and heart failure. Thus, the liver plays a role in remote immune regulation and is intricately linked with systemic immunity. This review provides a comprehensive overview of the liver remote regulation of the body’s innate and adaptive immunity regarding to main areas: immune-related molecules secreted by the liver and the liver-resident cells. Additionally, we assessed the influence of the liver on various facets of systemic immune-related diseases, offering insights into the clinical application of target therapies for liver immune regulation, as well as future developmental trends.

Published
2023
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3. Simple and Effective Primary Assessment of Emergency Patients in a COVID-19 Outbreak Area: A Retrospective, Observational Study

Author

Zhilin Wu, Gaohong Di, Chengkun Xia, Ling Hu, Jie Wang, Xiangdong Chen, and Shanglong Yao

Subjects
medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Isolation (health care), business.industry, 030503 health policy & services, Health Policy, Incidence (epidemiology), Risk of infection, Public Health, Environmental and Occupational Health, Outbreak, Retrospective cohort study, 03 medical and health sciences, 0302 clinical medicine, Health care, Pandemic, Emergency medicine, medicine, 030212 general & internal medicine, 0305 other medical science, business
Abstract

Background: The rapid spread of COVID-19 has expanded into a pandemic, for which the main containment strategies to reduce transmission are social distancing and isolation of ill persons Thousands of medical staff have been infected worldwide Coronavirus testing kits have been in short supply, and early diagnostic reagents did not have high sensitivity The aim of this study was to describe the characteristics of patients requiring emergency surgery in a COVID-19 outbreak area Methods: We assessed medical data regarding all patients who underwent emergency surgery at the main campus of Wuhan Union Hospital from January 23, 2020, to February 15, 2020 We classified patients based on suspicion of COVID-19 infection (suspected vs not suspected) before they were admitted to the operating room We used descriptive statistics to analyze the data Outcomes included the incidence of confirmed COVID-19 infection and length of stay, which were followed until March 25, 2020 Results: Among the 88 emergency patients included in this study, the mean age was 37 years Twenty-five patients presented with abnormalities observed on chest CT scans and 16 presented with fever The median wait time for surgery was one day The median preparation time and median time until short orientation memory concentration test (SOMCT) recovery from anesthesia were 44 0 min and 23 0 min, respectively The median postoperative length of stay was five days Compared with patients not suspected of COVID-19 infection, six patients were confirmed to be infected with COVID-19 in the suspected group No health care workers were infected during this study period Conclusion: Simple identification using temperature screening of patients, respiratory symptoms, and chest CT scans before being admitted for emergency surgery was rapid and effective Shortened contact times might reduce the risk of infection Additional investigations with larger samples and improved designs are needed to confirm these observations

Published
2020
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4. Simple and Effective Primary Assessment of Emergency Patients in a COVID-19 Outbreak Area: A Retrospective, Observational Study

Author

Gaohong, Di, Chengkun, Xia, Shanglong, Yao, Xiangdong, Chen, Zhilin, Wu, Ling, Hu, and Jie, Wang

Subjects
emergency, COVID-19, cross-infection, Original Research
Abstract

Background The rapid spread of COVID-19 has expanded into a pandemic, for which the main containment strategies to reduce transmission are social distancing and isolation of ill persons. Thousands of medical staff have been infected worldwide. Coronavirus testing kits have been in short supply, and early diagnostic reagents did not have high sensitivity. The aim of this study was to describe the characteristics of patients requiring emergency surgery in a COVID-19 outbreak area. Methods We assessed medical data regarding all patients who underwent emergency surgery at the main campus of Wuhan Union Hospital from January 23, 2020, to February 15, 2020. We classified patients based on suspicion of COVID-19 infection (suspected vs not suspected) before they were admitted to the operating room. We used descriptive statistics to analyze the data. Outcomes included the incidence of confirmed COVID-19 infection and length of stay, which were followed until March 25, 2020. Results Among the 88 emergency patients included in this study, the mean age was 37 years. Twenty-five patients presented with abnormalities observed on chest CT scans and 16 presented with fever. The median wait time for surgery was one day. The median preparation time and median time until short orientation memory concentration test (SOMCT) recovery from anesthesia were 44.0 min and 23.0 min, respectively. The median postoperative length of stay was five days. Compared with patients not suspected of COVID-19 infection, six patients were confirmed to be infected with COVID-19 in the suspected group. No health care workers were infected during this study period. Conclusion Simple identification using temperature screening of patients, respiratory symptoms, and chest CT scans before being admitted for emergency surgery was rapid and effective. Shortened contact times might reduce the risk of infection. Additional investigations with larger samples and improved designs are needed to confirm these observations.

Published
2020

6. Pt-confinement catalyst with dendritic hierarchical pores on excellent sulfur-resistance for hydrodesulfurization of dibenzothiophene and 4,6-dimethyldibenzothiophene

Author

Xilong Wang, Chengkun Xiao, Mohnnad H. Alabsi, Peng Zheng, Zhengkai Cao, Jinlin Mei, Yu Shi, Aijun Duan, Daowei Gao, Kuo-Wei Huang, and Chunming Xu

Subjects
Pt-confinement effect, Hydrogen spillover, Sulfur-resistance, Dibenzothiophenes, Hydrodesulfurization, Renewable energy sources, TJ807-830, Ecology, QH540-549.5
Abstract

Metal confinement catalyst MoS2/Pt@TD-6%Ti (TD, TS-1/Dendritic mesoporous silica nanoparticles composite) in dendritic hierarchical pore structures was synthesized and showed excellent sulfur-resistance performance and stabilities in catalytic hydrodesulfurization reactions of probe sulfide molecules. The MoS2/Pt@TD-6%Ti catalyst combines the concepts of Pt-confinement effect and hydrogen spillover of Pt noble metal. The modified micropores of Mo/Pt@TD-6%Ti only allow the migration and dissociation of small H2 molecules (0.289 nm), and effectively keep the sulfur-containing compounds (e.g. H2S, 0.362 nm) outside. Thus, the MoS2/Pt@TD-6%Ti catalyst exhibits higher DBT and 4,6-DMDBT HDS activities because of the synergistic effect of the strong H2 dissociation ability of Pt and desulfurization ability of MoS2 with a lower catalyst cost. This new concept combining H2 dissociation performance of noble metal catalyst with the desulfurization ability of transition metal sulfide MoS2 can protect the noble metal catalyst avoiding deactivation and poison, and finally guarantee the higher activities for DBT and 4,6-DMDBT HDS.

Published
2022
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8. Investigation on in vivo and in vitro blood compatibility of polypyrrole-coated titanium for cardiovascular application

Author

Kailun Zhang, Aini Xie, Chengkun Xia, Chenggui Liu, and Jianping Fan

Subjects
Materials science, technology, industry, and agriculture, Biomedical Engineering, chemistry.chemical_element, Substrate (chemistry), Biomaterial, respiratory system, engineering.material, equipment and supplies, Polypyrrole, chemistry.chemical_compound, Monomer, chemistry, Coating, Chemical engineering, In vivo, engineering, Surface modification, Titanium
Abstract

To identify the suitability of a conducting polymer-polypyrrole for development of a surface modification strategy of titanium, we electrocoated the monomer of pyrrole on titanium surface and produced an adherent polypyrrole coating. Then, this coating was characterised in terms of its mechanical and physical properties and also evaluated for in vivo and in vitro blood compatibility. It was found polypyrrole is more compatible than the bare titanium material with blood and possesses superior adhesion strength to titanium substrate and may serve as an ideal platform for anticoagulant functionalisation of titanium surface in the future.

Published
2012
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