1. Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter
- Author
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Michael Gonsior, Régis D. Gougeon, Maria Nikolantonaki, Jérémie Parot, Philippe Schmitt-Kopplin, Christian Coelho, Edith Parlanti, Procédés Alimentaires et Microbiologiques ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Physico -& Toxico Chimie des systèmes naturels ( LPTC ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Chesapeake Biological Laboratory ( CBL ), University of Maryland Center for Environmetal Science, Research Unit Analytical BioGeoChemistry ( BGC ), Helmholtz-Zentrum München ( HZM ), Chair of Analytical Food Chemistry, Technische Universität München [München] ( TUM ), Burgundy region, Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), UMR CNRS 5805 EPOC – OASU, Équipe LPTC, Université de Bordeaux, 351 Cours de la libération, 33405 Talence Cedex, France, Université de Bordeaux (UB), Chesapeake Biological Laboratory (CBL), University of Maryland Center for Environmental Science (UMCES), University of Maryland System-University of Maryland System, Research Unit Analytical BioGeoChemistry (BGC), Helmholtz-Zentrum München (HZM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), and Technical University of Munich (TUM)
- Subjects
Proteomics ,Proteome ,Size-exclusion chromatography ,polysaccharides ,Humic substances ,Wine ,White wine ,Wine proteins ,Fractionation ,Red wines ,01 natural sciences ,Biochemistry ,040501 horticulture ,Analytical Chemistry ,Chromophoric colloidal matter ,Colloid ,Molecular-weight ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Dissolved organic carbon ,Humans ,Colloids ,Mannoproteins ,Dissolved organic-matter ,Pressing ,Asymmetrical flow field-flow fractionation ,Chromatography ,Chemistry ,Elution ,digestive, oral, and skin physiology ,010401 analytical chemistry ,[ SDV.IDA ] Life Sciences [q-bio]/Food engineering ,food and beverages ,04 agricultural and veterinary sciences ,Fractionation, Field Flow ,Yeast ,0104 chemical sciences ,Maceration enzymes ,White Wine ,Haze-forming proteins ,0405 other agricultural sciences - Abstract
Two analytical separation methods-size-exclusion chromatography and asymmetrical flow field-flow fractionation-were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition. Wine chromophoric colloidal matter, representing UV-visible-absorbing wine macromolecules, was evaluated by optical and structural measurements combined with the description of elution profiles obtained by both separative techniques. The objective of this study was to apply these two types of fractionation on a typical Chardonnay white wine produced in Burgundy and to evaluate how each of them impacted the determination of the macromolecular chromophoric content of wine. UV-visible and fluorescence measurements of collected fractions were successfully applied. An additional proteomic study revealed that grape and microorganism proteins largely impacted the composition of chromophoric colloidal matter of Chardonnay wines. Asymmetrical flow field-flow fractionation appeared to be more reliable and less invasive with respect to the native chemical environment of chromophoric wine macromolecules, and hence is recommended as a tool to fractionate chromophoric colloidal matter in white wines. Graphical Abstract An innovative macromolecular separation method based on Asymmetrical Flow Field-Flow Fractionation was developed to better control colloidal dynamics across Chardonnay white winemaking.
- Published
- 2017