1. Expression-Based Cell Lineage Analysis in Drosophila Through a Course-Based Research Experience for Early Undergraduates.
- Author
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Olson JM, Evans CJ, Ngo KT, Kim HJ, Nguyen JD, Gurley KGH, Ta T, Patel V, Han L, Truong-N KT, Liang L, Chu MK, Lam H, Ahn HG, Banerjee AK, Choi IY, Kelley RG, Moridzadeh N, Khan AM, Khan O, Lee S, Johnson EB, Tigranyan A, Wang J, Gandhi AD, Padhiar MM, Calvopina JH, Sumra K, Ou K, Wu JC, Dickan JN, Ahmadi SM, Allen DN, Mai VT, Ansari S, Yeh G, Yoon E, Gon K, Yu JY, He J, Zaretsky JM, Lee NE, Kuoy E, Patananan AN, Sitz D, Tran P, Do MT, Akhave SJ, Alvarez SD, Asem B, Asem N, Azarian NA, Babaesfahani A, Bahrami A, Bhamra M, Bhargava R, Bhatia R, Bhatia S, Bumacod N, Caine JJ, Caldwell TA, Calica NA, Calonico EM, Chan C, Chan HH, Chang A, Chang C, Chang D, Chang JS, Charania N, Chen JY, Chen K, Chen L, Chen Y, Cheung DJ, Cheung JJ, Chew JJ, Chew NB, Chien CT, Chin AM, Chin CJ, Cho Y, Chou MT, Chow KK, Chu C, Chu DM, Chu V, Chuang K, Chugh AS, Cubberly MR, Daniel MG, Datta S, Dhaliwal R, Dinh J, Dixit D, Dowling E, Feng M, From CM, Furukawa D, Gaddipati H, Gevorgyan L, Ghaznavi Z, Ghosh T, Gill J, Groves DJ, Gurara KK, Haghighi AR, Havard AL, Heyrani N, Hioe T, Hong K, Houman JJ, Howland M, Hsia EL, Hsueh J, Hu S, Huang AJ, Huynh JC, Huynh J, Iwuchukwu C, Jang MJ, Jiang AA, Kahlon S, Kao PY, Kaur M, Keehn MG, Kim EJ, Kim H, Kim MJ, Kim SJ, Kitich A, Kornberg RA, Kouzelos NG, Kuon J, Lau B, Lau RK, Law R, Le HD, Le R, Lee C, Lee C, Lee GE, Lee K, Lee MJ, Lee RV, Lee SHK, Lee SK, Lee SD, Lee YJ, Leong MJ, Li DM, Li H, Liang X, Lin E, Lin MM, Lin P, Lin T, Lu S, Luong SS, Ma JS, Ma L, Maghen JN, Mallam S, Mann S, Melehani JH, Miller RC, Mittal N, Moazez CM, Moon S, Moridzadeh R, Ngo K, Nguyen HH, Nguyen K, Nguyen TH, Nieh AW, Niu I, Oh SK, Ong JR, Oyama RK, Park J, Park YA, Passmore KA, Patel A, Patel AA, Patel D, Patel T, Peterson KE, Pham AH, Pham SV, Phuphanich ME, Poria ND, Pourzia A, Ragland V, Ranat RD, Rice CM, Roh D, Rojhani S, Sadri L, Saguros A, Saifee Z, Sandhu M, Scruggs B, Scully LM, Shih V, Shin BA, Sholklapper T, Singh H, Singh S, Snyder SL, Sobotka KF, Song SH, Sukumar S, Sullivan HC, Sy M, Tan H, Taylor SK, Thaker SK, Thakore T, Tong GE, Tran JN, Tran J, Tran TD, Tran V, Trang CL, Trinh HG, Trinh P, Tseng HH, Uotani TT, Uraizee AV, Vu KKT, Vu KKT, Wadhwani K, Walia PK, Wang RS, Wang S, Wang SJ, Wiredja DD, Wong AL, Wu D, Xue X, Yanez G, Yang YH, Ye Z, Yee VW, Yeh C, Zhao Y, Zheng X, Ziegenbalg A, Alkali J, Azizkhanian I, Bhakta A, Berry L, Castillo R, Darwish S, Dickinson H, Dutta R, Ghosh RK, Guerin R, Hofman J, Iwamoto G, Kang S, Kim A, Kim B, Kim H, Kim K, Kim S, Ko J, Koenig M, LaRiviere A, Lee C, Lee J, Lung B, Mittelman M, Murata M, Park Y, Rothberg D, Sprung-Keyser B, Thaker K, Yip V, Picard P, Diep F, Villarasa N, Hartenstein V, Shapiro C, Levis-Fitzgerald M, Jaworski L, Loppato D, Clark IE, and Banerjee U
- Subjects
- Animals, Brain, Eye, Gene Expression, Lymphatic System, Research, Students, Universities, Wings, Animal, Cell Lineage, Drosophila genetics
- Abstract
A variety of genetic techniques have been devised to determine cell lineage relationships during tissue development. Some of these systems monitor cell lineages spatially and/or temporally without regard to gene expression by the cells, whereas others correlate gene expression with the lineage under study. The G AL4 T echnique for R eal-time a nd C lonal E xpression (G-TRACE) system allows for rapid, fluorescent protein-based visualization of both current and past GAL4 expression patterns and is therefore amenable to genome-wide expression-based lineage screens. Here we describe the results from such a screen, performed by undergraduate students of the University of California, Los Angeles (UCLA) Undergraduate Research Consortium for Functional Genomics (URCFG) and high school summer scholars as part of a discovery-based education program. The results of the screen, which reveal novel expression-based lineage patterns within the brain, the imaginal disc epithelia, and the hematopoietic lymph gland, have been compiled into the G-TRACE Expression Database (GED), an online resource for use by the Drosophila research community. The impact of this discovery-based research experience on student learning gains was assessed independently and shown to be greater than that of similar programs conducted elsewhere. Furthermore, students participating in the URCFG showed considerably higher STEM retention rates than UCLA STEM students that did not participate in the URCFG, as well as STEM students nationwide., (Copyright © 2019 Olson et al.)
- Published
- 2019
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