Search

Your search keyword '"Chibssa, Tesfaye Rufael"' showing total 17 results
Start Over Author "Chibssa, Tesfaye Rufael"
17 results on '"Chibssa, Tesfaye Rufael"'

1. Empirical and model-based evidence for a negligible role of cattle in peste des petits ruminants virus transmission and eradication

Author

Herzog, Catherine M., Aklilu, Fasil, Sibhatu, Demeke, Shegu, Dereje, Belaineh, Redeat, Mohammed, Abde Aliy, Kidane, Menbere, Schulz, Claudia, Willett, Brian J., Cleaveland, Sarah, Bailey, Dalan, Peters, Andrew R., Cattadori, Isabella M., Hudson, Peter J., Asgedom, Hagos, Buza, Joram, Forza, Mesfin Sahle, Chibssa, Tesfaye Rufael, Gebre, Solomon, Juleff, Nick, Bjørnstad, Ottar N., Baron, Michael D., and Kapur, Vivek

Published
2024
Full Text
View/download PDF

5. Harnessing Attenuation-Related Mutations of Viral Genomes: Development of a Serological Assay to Differentiate between Capripoxvirus-Infected and -Vaccinated Animals

Author

Berguido, Francisco J., primary, Chibssa, Tesfaye Rufael, additional, Loitsch, Angelika, additional, Liu, Yang, additional, Krstevski, Kiril, additional, Djadjovski, Igor, additional, Tuppurainen, Eeva, additional, Petrović, Tamaš, additional, Vidanović, Dejan, additional, Caufour, Philippe, additional, Settypalli, Tirumala Bharani K., additional, Grünwald-Gruber, Clemens, additional, Grabherr, Reingard, additional, Diallo, Adama, additional, Cattoli, Giovanni, additional, and Lamien, Charles Euloge, additional

Published
2023
Full Text
View/download PDF

8. Development and Optimization of Indirect ELISAs for the Detection of Anti-Capripoxvirus Antibodies in Cattle, Sheep, and Goat Sera

Author

Berguido, Francisco J., primary, Gelaye, Esayas, additional, Liu, Yang, additional, Davaasuren, Batdorj, additional, Krstevski, Kiril, additional, Djadjovski, Igor, additional, Ivanova, Emiliya, additional, Goujgoulova, Gabriela, additional, Loitsch, Angelika, additional, Tuppurainen, Eeva, additional, Chibssa, Tesfaye Rufael, additional, Caufour, Philippe, additional, Samojlović, Milena, additional, Lazić, Sava, additional, Petrović, Tamaš, additional, Vidanović, Dejan, additional, Bertagnoli, Stéphane, additional, Grabherr, Reingard, additional, Diallo, Adama, additional, Cattoli, Giovanni, additional, and Lamien, Charles Euloge, additional

Published
2022
Full Text
View/download PDF

9. Community Wastewater-Based Surveillance Can Be a Cost-Effective Approach to Track COVID-19 Outbreak in Low-Resource Settings: Feasibility Assessment for Ethiopia Context

Author

Ali, Solomon, primary, Gudina, Esayas Kebede, additional, Gize, Addisu, additional, Aliy, Abde, additional, Adankie, Birhanemeskel Tegene, additional, Tsegaye, Wondwossen, additional, Hundie, Gadissa Bedada, additional, Muleta, Mahteme Bekele, additional, Chibssa, Tesfaye Rufael, additional, Belaineh, Rediet, additional, Negessu, Demessa, additional, Shegu, Dereje, additional, Froeschl, Guenter, additional, and Wieser, Andreas, additional

Published
2022
Full Text
View/download PDF

11. Porcine circovirus‐2 in Africa: Identification of continent‐specific clusters and evidence of independent viral introductions from Europe, North America and Asia

Author

Franzo, Giovanni, primary, Settypalli, Tirumala B.K., additional, Agusi, Ebere Roseann, additional, Meseko, Clement, additional, Minoungou, Germaine, additional, Ouoba, Bruno Lalidia, additional, Habibata, Zerbo Lamouni, additional, Wade, Abel, additional, de Barros, José Luís, additional, Tshilenge, Curé Georges, additional, Gelaye, Esayas, additional, Yami, Martha, additional, Gizaw, Daniel, additional, Chibssa, Tesfaye Rufael, additional, Anahory, Iolanda Vieira, additional, Mapaco, Lourenço P., additional, Achá, Sara J., additional, Ijomanta, Jeremiah, additional, Jambol, Anvou Rachael, additional, Adedeji, Adeyinka Jeremy, additional, Luka, Pam Dachung, additional, Shamaki, David, additional, Diop, Mariame, additional, Bakhoum, Mame Thierno, additional, Lo, Modou Moustapha, additional, Chang'a, Jelly S., additional, Magidanga, Bishop, additional, Mayenga, Charles, additional, Ziba, Maureen Wakwamba, additional, Dautu, George, additional, Masembe, Charles, additional, Achenbach, Jenna, additional, Molini, Umberto, additional, Cattoli, Giovanni, additional, Lamien, Charles E., additional, and Dundon, William G., additional

Published
2021
Full Text
View/download PDF

12. Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro

Author

Chibssa, Tesfaye Rufael, primary, Kangethe, Richard Thiga, additional, Berguido, Francisco J., additional, Settypalli, Tirumala Bharani K., additional, Liu, Yang, additional, Grabherr, Reingard, additional, Loitsch, Angelika, additional, Sassu, Elena Lucia, additional, Pichler, Rudolf, additional, Cattoli, Giovanni, additional, Diallo, Adama, additional, Wijewardana, Viskam, additional, and Lamien, Charles Euloge, additional

Published
2021
Full Text
View/download PDF

13. Molecular Analysis of East African Lumpy Skin Disease Viruses Reveals a Mixed Isolate with Features of Both Vaccine and Field Isolates

Author

Chibssa, Tesfaye Rufael, primary, Sombo, Melaku, additional, Lichoti, Jacqueline Kasiiti, additional, Adam, Tajelser Idris Badri, additional, Liu, Yang, additional, Elraouf, Yazeed Abd, additional, Grabherr, Reingard, additional, Settypalli, Tirumala Bharani K., additional, Berguido, Francisco J., additional, Loitsch, Angelika, additional, Sahle, Mesfin, additional, Cattoli, Giovanni, additional, Diallo, Adama, additional, and Lamien, Charles Euloge, additional

Published
2021
Full Text
View/download PDF

14. Porcine circovirus‐2 in Africa: Identification of continent‐specific clusters and evidence of independent viral introductions from Europe, North America and Asia.

Author

Franzo, Giovanni, Settypalli, Tirumala B.K., Agusi, Ebere Roseann, Meseko, Clement, Minoungou, Germaine, Ouoba, Bruno Lalidia, Habibata, Zerbo Lamouni, Wade, Abel, de Barros, José Luís, Tshilenge, Curé Georges, Gelaye, Esayas, Yami, Martha, Gizaw, Daniel, Chibssa, Tesfaye Rufael, Anahory, Iolanda Vieira, Mapaco, Lourenço P., Achá, Sara J., Ijomanta, Jeremiah, Jambol, Anvou Rachael, and Adedeji, Adeyinka Jeremy

Subjects
SWINE, VIRAL proteins, VIRAL genes, GENOTYPES, DNA
Abstract

Porcine circovirus‐2 (PCV‐2) is associated with several disease syndromes in domestic pigs that have a significant impact on global pig production and health. Currently, little is known about the status of PCV‐2 in Africa. In this study, a total of 408 archived DNA samples collected from pigs in Burkina Faso, Cameroon, Cape Verde, Ethiopia, the Democratic Republic of the Congo, Mozambique, Nigeria, Senegal, Tanzania and Zambia between 2000 and 2018 were screened by PCR for the presence of PCV‐2. Positive amplicons of the gene encoding the viral capsid protein (ORF2) were sequenced to determine the genotypes circulating in each country. Four of the nine currently known genotypes of PCV‐2 were identified (i.e. PCV‐2a, PCV‐2b, PCV‐2d and PCV‐2 g) with more than one genotype being identified in Burkina Faso, Ethiopia, Nigeria, Mozambique, Senegal and Zambia. Additionally, a phylogeographic analysis which included 38 additional ORF2 gene sequences of PCV‐2s previously identified in Mozambique, Namibia and South Africa from 2014 to 2016 and 2019 to 2020 and available in public databases, demonstrated the existence of several African‐specific clusters and estimated the approximate time of introduction of PCV‐2s into Africa from other continents. This is the first in‐depth study of PCV‐2 in Africa and it has important implications for pig production at both the small‐holder and commercial farm level on the continent. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

15. Use of an Alignment-Free Method for the Geographical Discrimination of GTPVs Based on the GPCR Sequences

Author

Chibssa, Tesfaye Rufael, primary, Liu, Yang, additional, Sombo, Melaku, additional, Lichoti, Jacqueline Kasiiti, additional, Erdenebaatar, Janchivdorj, additional, Boldbaatar, Bazartseren, additional, Grabherr, Reingard, additional, Settypalli, Tirumala Bharani K., additional, Berguido, Francisco J., additional, Loitsch, Angelika, additional, Damena, Delesa, additional, Cattoli, Giovanni, additional, Diallo, Adama, additional, and Lamien, Charles Euloge, additional

Published
2021
Full Text
View/download PDF

17. Differentiation of sheep poxvirus vaccines from field isolates and other Capripoxvirus species

Author

Cattoli, Giovanni, Chibssa, Tesfaye Rufael, Settypalli, Tirumala Bharani Kumar, Berguido, Francisco, Loitsch, Angelika, Diallo, Adama, and Euloge Lamien, Charles

Abstract

Background The genus Capripoxvirus (CaPV) within the family Poxviridae com-prises three closely related viruses, sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) causing sheep pox (SPP), goat pox (GTP) and lumpy skin disease (LSD) in small ru-minants and cattle respectively. LSD has emerged in Europe in 2015 and first incursions of SPP in the European Union were reported in Bulgaria and Greece in 2013. Live attenuated SPPV vaccines are widely used in many countries to control SPP and GTP. With the in-creasing number of reports on SPP in previously vaccinated sheep herds, it is imperative to develop new diagnostic tools for differen-tiation of SPPV field strains from attenuated vaccine strains. Objective This work aimed at identifying appropriate diagnostic targets to de-velop assays for the rapid and accurate differentiation SPPV vaccine strains from SPPV field isolates and other CaPVs. Methodology To identify a suitable molecular target for the development of these assays, the full genomes of several SPPV vaccines strains and SPPV field isolates were compared. A unique 84-base pair nucleotide dele-tion located between the DNA ligase and the B22R gene was exploit-ed to develop a gel-based PCR, and a region containing a 48bp de-letion within the B22R gene of SPPV vaccines strains only, as well as species-specific nucleotide difference between SPPV field isolates, GTPV and LSDV, was targeted to develop a HRM assay. Results The gel-based assay was readily able to differentiate SPPV vaccines from field isolates. However, this method alone could not differen-tiate SPPV field isolates from GTPV and LSDV. In contrast, the HRM based method allowed the differentiation of SPPV vaccines from field isolates and further enabled the genotyping of capripoxviruses isolates. Out of 61 samples tested, we identified 4 SPPV vaccines, 14 SPPV field isolates, 11 GTPVs and 32 LSDVs. The two assays were both sensitive and specific and in agreement with the sequencing data of the tested samples. Conclusion The assays described herein are reliable and rapid methods for the differentiation of SPPV Vaccines from SPPV field isolates. While the gel based assay needs to be combined with capripoxvirus spe-cies-specific assays, the HRM assay stands alone as a tool to differen-tiate SPPV vaccines from field isolates and simultaneously genotype SPPVs, GTPVs and LSDVs. The methods are suited for routine use during outbreak investigations in both capripoxvirus enzootic and disease-free countries.

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results