Background: Anxiety is a common issue among pre-surgery patients, and also popular explored among medical surgical patients. But for gynecologic laparotomy patients' pre-surgery anxiety and the effects of the nursing education for anxiety still rare. Purpose: The purpose of this study was to examine the effects of multimedia education program on pre-surgery anxiety in gynecologic laparotomy patients. Methods: Using a two groups compared study design. All participants were over 20 years of age and will receiving gynecologic laparotomy. Participants were distributed into with a multimedia education group (experimental group, n = 100) or with pamphlet group (control group, n = 100). Outcome measurements included state-trait anxiety inventory, disease related data, and personal demographics. We examined outcome indicators before nursing intervention and follow up at one hour after nursing intervention. Results: A post-test comparison of the two groups showed a statistically significant intergroup difference in terms of both state-trait anxiety and breathing (p < .05). In addition, a mean differential comparison showed statistically significant differences between the groups in terms of state-trait anxiety, blood pressure, pulse rate, and breathing rate (p < .05). Conclusions / Implications for Practice: This study demonstrates the efficacy of multimedia education in decreasing pre-surgery anxiety in gynecologic laparotomy patients. Findings may be referenced in the development of multimedia nursing instructions. The authors suggest that these instructions may be disseminated via hospital websites to allow patients to read prior to admission in order to increase readiness and reduce surgery-related anxiety. [ABSTRACT FROM AUTHOR]