252 results on '"Ching-Yi Lin"'
Search Results
2. The Spatiotemporal Expression of SOCS3 in the Brainstem and Spinal Cord of Amyotrophic Lateral Sclerosis Mice
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Ching-Yi Lin, Veronica Vanoverbeke, David Trent, Kathryn Willey, and Yu-Shang Lee
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amyotrophic lateral sclerosis (ALS) ,suppressor of cytokine signaling-3 (SOCS3) ,pre-Bötzinger Complex (preBötC) ,neuroinflammation ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Amyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of motor neurons from the brain and spinal cord. The excessive neuroinflammation is thought to be a common determinant of ALS. Suppressor of cytokine signaling-3 (SOCS3) is pathologically upregulated after injury/diseases to negatively regulate a broad range of cytokines/chemokines that mediate inflammation; however, the role that SOCS3 plays in ALS pathogenesis has not been explored. Here, we found that SOCS3 protein levels were significantly increased in the brainstem of the superoxide dismutase 1 (SOD1)-G93A ALS mice, which is negatively related to a progressive decline in motor function from the pre-symptomatic to the early symptomatic stage. Moreover, SOCS3 levels in both cervical and lumbar spinal cords of ALS mice were also significantly upregulated at the pre-symptomatic stage and became exacerbated at the early symptomatic stage. Concomitantly, astrocytes and microglia/macrophages were progressively increased and reactivated over time. In contrast, neurons were simultaneously lost in the brainstem and spinal cord examined over the course of disease progression. Collectively, SOCS3 was first found to be upregulated during ALS progression to directly relate to both increased astrogliosis and increased neuronal loss, indicating that SOCS3 could be explored to be as a potential therapeutic target of ALS.
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- 2024
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3. Measuring and modeling macrophage proliferation in a lab-on-CMOS capacitance sensing microsystem
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Kyle Smith, Ching-Yi Lin, Yann Gilpin, Elizabeth Wayne, and Marc Dandin
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CMOS ,biosensors ,capacitance ,lab-on-chip ,lab-on-CMOS ,model ,Biotechnology ,TP248.13-248.65 - Abstract
We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the proliferation of RAW 264.7 murine Balb/c macrophages. We show that macrophage proliferation correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of electrodes dispersed in a sensing area of interest. We further show a temporal model that captures the cell number evolution in the area over long periods (e.g., 30 h). The model links the cell numbers and the average capacitance growth factor to describe the observed cell proliferation.
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- 2023
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4. Embryonic and post-embryonic development in the parasitic copepod Ive ptychoderae (Copepoda: Iviidae): Insights into its phylogenetic position.
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Yu-Rong Cheng, Ching-Yi Lin, and Jr-Kai Yu
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Medicine ,Science - Abstract
Parasitic copepods are frequently discovered in many marine animals, and they exhibit great species diversity with remarkable morphological adaptations to their parasitic lifestyle. Similar to their free-living relatives, parasitic copepods usually develop through complex life cycle, but they eventually transform into a modified adult form with reduced appendages. Although the life cycle and distinct larval stages have been described in a few species of parasitic copepods, particularly those infecting commercially valuable marine animals (such as fishes, oysters, and lobsters), very little is known about the developmental process of the species that transformed into extremely simplified adult body plan. This paucity also causes some difficulties when investigating the taxonomy and phylogeny of this kind of parasitic copepods. Here we describe the embryonic development and a series of sequential larval stages of a parasitic copepod, Ive ptychoderae, which is a vermiform endoparasite living inside the hemichordate acorn worms. We devised laboratory regimes that enable us raising large quantity of embryos and free living larvae, and obtaining post-infested I. ptychoderae samples from the host tissues. Using defined morphological features, the embryonic development of I. ptychoderae can be categorized into eight stages (1-, 2-, 4-, 8-, 16- cell stages, blastula, gastrula, and limb bud stages) and the post-embryonic development comprises six larval stages (2 naupliar and 4 copepodid stages). Based on the comparisons of morphological characters in the nauplius stage, our results provide evidence to support that the Ive-group is more closely related to the Cyclopoida, which represents one of the two major clades that contain many highly transformed parasitic copepods. Thus, our results help to resolve the problematic phylogenetic position of the Ive-group in previous study based on analysis using 18S rDNA sequences. Combining with more molecular data, future comparative analyses on the morphological features of copepodid stages will further refine our understanding of the phylogenetic relationships of parasitic copepods.
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- 2023
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5. Mitochondrial haplogroups have a better correlation to insulin requirement than nuclear genetic variants for type 2 diabetes mellitus in Taiwanese individuals
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Feng‐Chih Shen, Shao‐Wen Weng, Meng‐Han Tsai, Yu‐Jih Su, Sung‐Chou Li, Shun‐Jen Chang, Jung‐Fu Chen, Yen‐Hsiang Chang, Chia‐Wei Liou, Tsu‐Kung Lin, Jiin‐Haur Chuang, Ching‐Yi Lin, and Pei‐Wen Wang
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Diabetes ,Insulin ,Mitochondria ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ABSTRACT Aims/Introduction Identifying diabetes‐susceptible genetic variants will help to provide personalized therapy for the management of type 2 diabetes. Previous studies have reported a genetic risk score (GRS), computed by the sum of nuclear DNA (nDNA) risk alleles, that may predict the future requirement for insulin therapy. Although mitochondrial dysfunction has a close association with insulin resistance (IR), there are few studies investigating whether genetic variants of mitochondrial DNA (mtDNA) will affect the clinical characteristics of type 2 diabetes. Materials and Methods Mitochondrial haplogroups were determined using mtDNA whole genome next generation sequencing and 13 single nucleotide polymorphisms (SNPs) in nDNA susceptibility loci of 13 genes in 604 Taiwanese subjects with type 2 diabetes. A GRS of nDNA was computed by summation of the number of risk alleles. The correlation between the mtDNA haplogroup and the clinical characteristics of type 2 diabetes was assessed by logistic regression analysis. The results were compared with the GRS subgroups for the risk of insulin requirement. Results Mitochondrial haplogroups modulate the clinical characteristics of type 2 diabetes, in which patients harboring haplogroup D4, compared with those harboring non‐D4 haplotypes, were less prone to require insulin treatment, after adjusting for age, gender, and diabetes duration. However, there was no association between insulin requirement and GRS calculated from nuclear genetic variants. Conclusions Mitochondrial haplogroups, but not nuclear genetic variants, have a better association with the insulin requirement. The results highlight the role of mitochondria in the management of common metabolic diseases.
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- 2022
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6. Impact of periodontal disease and chewing ability on the quality of life of the elderly in an affluent community
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Ya-Hong Liang, Chyuan Chou, Ying-Jen Chen, Yen-Fang Chou, Ching-Yi Lin, Caroline Chou, and Tze-Fang Wang
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Chewing ability ,Periodontal disease ,Oral health-related quality of life ,Older adults ,Affluent community ,Medicine (General) ,R5-920 - Abstract
Background/Purpose: Improvement of the medical and social environment is an important aspect of government policies in assuring the quality of life (QoL) of older adults. However, few studies have appraised the impact and relationship of QoL with clinical factors among elderly individuals in affluent residential areas. Methods: A total of 166 older adults from Chang Gung Health and Culture Village were enrolled. Oral health-related QoL was measured using the Geriatric Oral Health Assessment Index. Self-assessed chewing abilities and demographic characteristics were collected by questionnaire. Physical health was scored by activities of daily living (ADL) and instrumental ADL. Mini nutrition assessment questionnaire was used to evaluate the nutritional intake. Multiple linear regression was used to predict risk factors affecting QoL and to examine whether chewing ability was a mediator of oral health-related QoL. Results: Poor chewing ability was associated with older age (OR = 1.82 for 76–85 years and 3.58 for 86–95 years), sufficient economic status (OR = 5.55) and removable denture-wearing (OR = 7.52). On the other hand, poor chewing ability (OR = 0.11), removable denture-wearing (OR = 0.48), periodontal disease (OR = 0.38) had lower likelihood of good oral health-related QoL. Mediator analyses showed that chewing ability was a mediator for the association between oral health-related QoL and economic status or removable denture-wearing or having more than 20 teeth. Periodontal disease was an independent factor for oral health-related QoL. Conclusion: Among older adults in an affluent community, periodontal disease is an independent risk factor directly related to oral health-related QoL, whereas chewing ability is a mediator between QoL and removable denture-wearing.
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- 2020
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7. Changes in drowning mortality rates and quality of reporting from 2004–2005 to 2014–2015: a comparative study of 61 countries
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Ching-Yi Lin, Liang-Yi Wang, and Tsung-Hsueh Lu
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Drowning ,Accidents ,Death certificate ,Mortality ,International comparisons ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background This study assessed international variations in changes in drowning mortality rates and the quality of reporting specific information in death certificates over the past decade. Methods Drowning mortality data of 61 countries were extracted from the World Health Organization Mortality Database. We calculated the percentage change (PC) in age-standardized drowning mortality rates and percentage of drowning deaths reported with unspecified codes between 2004 and 2005 and 2014–2015. Results Of the 61 countries studied, 50 exhibited a reduction in drowning mortality rates from 2004 to 2005 to 2014–2015. Additionally, five countries—Lithuania, Moldova, Kyrgyzstan, Romania, and El Salvador—with a high mortality rate in 2004–2005 (> 40 deaths per 100,000) showed improvement (PC 40%) exhibited a marked reduction (PC
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- 2019
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8. Interactions between Obstructive Sleep Apnea Syndrome Severity, Obesity, Sex Difference and Attention-Deficit/Hyperactivity Disorder on Health-Related Quality of Life: A Non-Interventional Prospective Observational Study
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Yu-Hsuan Chen, Ming-Feng Wu, Chih-Yu Wen, Shih-Pei Chang, Ching-Yi Lin, Yi-Chan Chen, Ching-Cheng Lin, Hui-Chen Chen, Wei-Chang Huang, and Kai-Ming Chang
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attention-deficit/hyperactivity disorder ,health-related quality of life ,obstructive sleep apnea syndrome ,Biology (General) ,QH301-705.5 - Abstract
Obstructive sleep apnea syndrome (OSAS) severity, obesity, sex difference, and attention-deficit/hyperactivity disorder (ADHD) had a complex impact on health-related quality of life (HRQoL). However, the interactive effects among these features on HRQoL remained to be clarified. This study aimed to investigate the individual and interactive associations between the four characteristics of interest and HRQoL as determined by 36-Item Short Form Health Survey, Pittsburgh Sleep Quality Index (PSQI), and Epworth Sleepiness Scale (ESS). This non-interventional, prospective, observational study enrolled a total of 132 patients with suspected OSAS for analysis. While OSAS severity and ADHD detected by adult ADHD Self-Report Scale, termed as screened ADHD, interact with each other, all the four studied features were individually associated with HRQoL. After adjusting for potential physiological and polysomnographic confounders, screened ADHD was independently correlated with PSQI > 5 (OR = 4.126, 95% CI, 1.490–11.424), mental component score < 50 (OR = 5.873, 95% CI, 2.262–15.251) and ESS > 10 (OR = 3.648, 95% CI, 1.738–7.657). Our results show that ADHD detection is necessary and should be incorporated into clinical practice for OSAS management.
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- 2022
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9. Changes in the number and causes of maternal deaths after the introduction of pregnancy checkbox on the death certificate in Taiwan
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Ching-Yi Lin, Pei-Yin Tsai, Liang-Yi Wang, Ginden Chen, Pao-Lin Kuo, Meng-Chih Lee, and Tsung-Hueh Lu
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Gynecology and obstetrics ,RG1-991 - Abstract
Objective: To examine changes in the number and causes of maternal deaths after the introduction of pregnancy checkbox on the death certificate in January 2014 in Taiwan. Materials and methods: We first used the cause-of-death (COD) mortality data for years 2010 through 2017 to examine the number of deaths by item of pregnancy checkbox. We then compared the distribution of the causes of maternal deaths before and after the introduction of pregnancy checkbox. Results: Between 2014 and 2017, 111 women died, for whom the certifiers indicated the following in the pregnancy checkbox items: 2 (pregnant at the time of death; n = 10), 3 (died within 42 days after the termination of pregnancy; n = 64), and 4 (died between 43 days and 1 year after the termination of pregnancy; n = 37). However, in only 61 of the 111 deaths, the certifiers reported pregnancy or delivery-related diagnosis in the COD section of the death certificate—5 each for items 2 and 4 and 51 for item 3. The number of maternal deaths was 55 in 2010–2013; this number increased to 82 in 2014–2017. A decline in the percentage of maternal deaths from obstetric hemorrhage was noted from 38% (21/55) in 2010–2013 to 21% (17/82) in 2014–2017. Conclusion: The number of maternal deaths increased, and the distribution of causes of maternal deaths changed after the introduction of pregnancy checkbox. Additional studies are required to examine the possible misclassification of pregnancy-associated deaths indicated in the pregnancy checkbox. Keywords: Cause-of-death, Death certificate, Pregnancy checkbox, Pregnancy-associated deaths, Maternal deaths
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- 2019
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10. Uses of dietary supplements and herbal medicines during pregnancy in women undergoing assisted reproductive technologies– A study of taiwan birth cohort
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Ching-Yi Lin, Yu-Ju Chen, Shu-Hsin Lee, Ching-Pyng Kuo, Maw-Sheng Lee, and Meng-Chih Lee
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Gynecology and obstetrics ,RG1-991 - Abstract
Objective: This study aimed to assess the efficacy of dietary supplements and herbal medicines for the care of pregnant women undergoing assisted reproductive technologies (ART). Materials and methods: A total of 366 women undergoing ART and their children from the dataset of Taiwan Birth Cohort Study (TBCS, 2005) were enrolled in this study. Structured questionnaires were applied to collect the health information at 6-month follow-up after their delivery. The related use patterns were analyzed to investigate the final birth outcomes. Results: Comparing with those of non-ART group, the women undergoing ART consumed more supplements of multivitamin, fish oil, and calcium than herbal medicines during pregnancy. This study revealed that the consumptions of multivitamin, calcium pills, Genseng, and Suz-Wu-Tang were associated with low birth weight, whereas the intake of Huanglian was associated with birth weight. Besides, the uses of multivitamin and Suz-Wu-Tang were related to lower gestational age of infants. Conclusions: Physicians and nurses must educate themselves in dietary supplements and herbal/alternative medicines for offering accurate advices for pregnant women to optimize their care. The results could be of reference for further investigation on longitudinal effects of dietary supplements and herbal medicines during pregnancy in women undergoing ART continuously followed with TBCS. Keywords: Assisted reproductive technologies (ART), Dietary supplements, Herbal medicines, Pregnancy, Postpartum
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- 2019
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11. Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells
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Yi-Hsien Hsieh, Fang-Jung Yu, Yasser Nassef, Chung-Jung Liu, Yong-Syuan Chen, Ching-Yi Lin, Jia-Liang Feng, and Min-Hua Wu
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prostate cancer ,growth ,apoptosis ,miRNA-3614-5p ,Mcl-1 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
MicroRNA (miRNA) acts as a critical regulator of growth in various human malignancies. However, the role of miRNA-3614 in the progression of human prostate cancer remains unknown. In this study, our results demonstrated that miRNA-3614-5p exerts a significant inhibitory effect on cell viability and colony formation and induces sub-G1 cell cycle arrest and apoptosis in human prostate cancer cells. Myeloid cell leukemia-1 (Mcl-1) acts as a master regulator of cell survival. Using the miRNA databases, miRNA-3614-5p was found to regulate Mcl-1 expression by targeting positions of the Mcl-1-3′ UTR. The reduction of Mcl-1 expression by miRNA-3614-5p was further confirmed using an immunoblotting assay. Pro-apoptotic caspase-3 and poly (ADP-ribose) polymerase (PARP) were significantly activated by miRNA-3614-5p to generate cleaved caspase-3 (active caspase-3) and cleaved PARP (active PARP), accompanied by the inhibited Mcl-1 expression. These findings were the first to demonstrate the anti-growth effects of miRNA-3614-5p through downregulating Mcl-1 expression in human prostate cancer cells.
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- 2022
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12. The Causal Role of Mitochondrial Dynamics in Regulating Innate Immunity in Diabetes
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Yen-Hsiang Chang, Hung-Yu Lin, Feng-Chih Shen, Yu-Jih Su, Jiin-Haur Chuang, Tsu-Kung Lin, Chia-Wei Liou, Ching-Yi Lin, Shao-Wen Weng, and Pei-Wen Wang
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mitochondrial dynamics ,innate immunity ,type 2 diabetes ,nutrient excess ,inflammation ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Background: Plenty of evidence suggested that chronic low-grade inflammation triggered by innate immunity activation contributes to the pathogenesis of type 2 diabetes (T2D). Using the trans-mitochondrial cybrid cell model, we have demonstrated that mitochondria independently take part in the pathological process of insulin resistance (IR) and pro-inflammatory phenotype in cybrid cells harboring mitochondrial haplogroup B4, which are more likely to develop T2D. The mitochondrial network is more fragmented, and the expression of fusion-related proteins is low in Cybrid B4. We also discovered the causal role of mitochondrial dynamics (mtDYN) proteins in regulating IR in this cybrid model, and the bidirectional interaction between mtDYN and mitochondrial oxidative stress is considered etiologically important. In this study, we further investigated whether mtDYN bridges the gap between nutrient excess and chronic inflammation in T2D.Methods: Trans-mitochondrial cybrid cells derived from the 143B human osteosarcoma cell line were cultured in a medium containing glucose (25 mM) with or without saturated fatty acid (0.25 mM BSA-conjugated palmitate), and the expression of innate immunity/inflammasome molecules was compared between cybrid B4 (the major T2D-susceptible haplogroup among the Chinese population) and cybrid D4 (the major T2D-resistant haplogroup among the Chinese population). We investigated the causal relationship between mtDYN and nutrient excess-induced inflammation in cybrid B4 by genetic manipulation of mtDYN and by pharmacologically inhibiting mitochondrial fission using the Drp1 inhibitor, mdivi-1, and metformin.Results: Under nutrient excess with high fatty acid, cybrid B4 presented increased mitochondrial pro-fission profiles and enhanced chronic inflammation markers (RIG-I, MDA5, MAVS) and inflammasome (NLRP3, Caspase-1, IL-1β), whereas the levels in cybrid D4 were not or less significantly altered. In cybrid B4 under nutrient excess, overexpression of fusion proteins (Mfn1 or Mfn2) significantly repressed the expression of innate immunity/inflammasome-related molecules, while knockdown had a less significant effect. On the contrary, knockdown of fission proteins (Drp1 or Fis1) significantly repressed the expression of innate immunity/inflammasome-related molecules, while overexpression had a less significant effect. In addition, Drp1 inhibitor mdivi-1 and metformin inhibited mitochondrial fission and attenuated the pro-inflammation expression as well.Conclusion: Our results discovered the causal relationship between mtDYN and nutrient excess-induced chronic inflammation in a diabetes-susceptible cell model. Targeting mtDYN by direct interfering pro-fission can be a therapeutic intervention for chronic inflammation in T2D.
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- 2020
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13. Ionizing Radiation Induces Resistant Glioblastoma Stem-Like Cells by Promoting Autophagy via the Wnt/β-Catenin Pathway
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Cheng-Yu Tsai, Huey-Jiun Ko, Chi-Ying F. Huang, Ching-Yi Lin, Shean-Jaw Chiou, Yu-Feng Su, Ann-Shung Lieu, Joon-Khim Loh, Aij-Lie Kwan, Tsung-Hsien Chuang, and Yi-Ren Hong
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GBM ,CSC ,ionizing radiation (IR) ,GSC ,Wnt/β-Catenin ,autophagy ,Science - Abstract
Therapeutic resistance in recurrent glioblastoma multiforme (GBM) after concurrent chemoradiotherapy (CCRT) is a challenging issue. Although standard fractionated radiation is essential to treat GBM, it has led to local recurrence along with therapy-resistant cells in the ionizing radiation (IR) field. Lines of evidence showed cancer stem cells (CSCs) play a vital role in therapy resistance in many cancer types, including GBM. However, the molecular mechanism is poorly understood. Here, we proposed that autophagy could be involved in GSC induction for radioresistance. In a clinical setting, patients who received radiation/chemotherapy had higher LC3II expression and showed poor overall survival compared with those with low LC3 II. In a cell model, U87MG and GBM8401 expressed high level of stemness markers CD133, CD44, Nestin, and autophagy marker P62/LC3II after receiving standard fractionated IR. Furthermore, Wnt/β-catenin proved to be a potential pathway and related to P62 by using proteasome inhibitor (MG132). Moreover, pharmacological inhibition of autophagy with BAF and CQ inhibit GSC cell growth by impairing autophagy flux as demonstrated by decrease Nestin, CD133, and SOX-2 levels. In conclusion, we demonstrated that fractionated IR could induce GSCs with the stemness phenotype by P62-mediated autophagy through the Wnt/β-catenin for radioresistance. This study offers a new therapeutic strategy for targeting GBM in the future.
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- 2021
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14. Combinatory repair strategy to promote axon regeneration and functional recovery after chronic spinal cord injury
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Marc A. DePaul, Ching-Yi Lin, Jerry Silver, and Yu-Shang Lee
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Medicine ,Science - Abstract
Abstract Eight weeks post contusive spinal cord injury, we built a peripheral nerve graft bridge (PNG) through the cystic cavity and treated the graft/host interface with acidic fibroblast growth factor (aFGF) and chondroitinase ABC (ChABC). This combinatorial strategy remarkably enhanced integration between host astrocytes and graft Schwann cells, allowing for robust growth, especially of catecholaminergic axons, through the graft and back into the distal spinal cord. In the absence of aFGF+ChABC fewer catecholaminergic axons entered the graft, no axons exited, and Schwann cells and astrocytes failed to integrate. In sharp contrast with the acutely bridge-repaired cord, in the chronically repaired cord only low levels of serotonergic axons regenerated into the graft, with no evidence of re-entry back into the spinal cord. The failure of axons to regenerate was strongly correlated with a dramatic increase of SOCS3 expression. While regeneration was more limited overall than at acute stages, our combinatorial strategy in the chronically injured animals prevented a decline in locomotor behavior and bladder physiology outcomes associated with an invasive repair strategy. These results indicate that PNG+aFGF+ChABC treatment of the chronically contused spinal cord can provide a permissive substrate for the regeneration of certain neuronal populations that retain a growth potential over time, and lead to functional improvements.
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- 2017
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15. A simple route to linear and hyperbranched polythiophenes containing diketopyrrolopyrrole linking groups with improved conversion efficiency
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Chia-Hao Hsieh, Wei-Chi Chen, Sheng-Hsiung Yang, Yu-Chiang Chao, Hsiao-Chin Lee, Chia-Ling Chiang, and Ching-Yi Lin
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hyperbranched ,polythiophene ,diketopyrrolopyrrole ,inverted polymer solar cells ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Two novel polythiophene derivatives with linear or hyperbranched architectures using diketopyrrolopyrrole (DPP) as linking groups as well as normal poly(3-hexylthiophene) (P3HT) were synthesized via the Universal Grignard metathesis polymerization. The molecular weights of the linear polythiophene containing DPP linking moieties are higher than those of P3HT, while molecular weights of the hyperbranched one are smaller. The main decomposition temperatures of polymers were measured at 470 °C from TGA experiments. The UV-vis absorption behaviors of the DPP-containing polymers are similar to that of P3HT; moreover, these DPP-containing polythiophenes show distinct PL decay both in solution and thin film states. The electrochemical experiments reveal that the incorporation of DPP groups resulted in lowering HOMO levels of polymers. All polymers were blended with PC61BM and used as active layers for the fabrication of inverted polymer solar cells. The power conversion efficiency of devices based on linear and hyperbranched polythiophenes reached 3.74% and 2.38%, respectively, revealing comparable or even higher efficiency than the one based on normal P3HT.
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- 2017
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16. Verification and Evaluation of a Visual Reaction System for Badminton Training
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Kuei-Pin Kuo, Hsun-Heng Tsai, Ching-Yi Lin, and Wei-Te Wu
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agility ,competitive environment ,footwork drills ,simulation ,Chemical technology ,TP1-1185 - Abstract
The two aims of this study were (1) designing and developing an affordable visual reaction system for badminton training that monitors and provides instant feedback on agility; and (2) to measure and improve the footwork and movement of badminton players and output useful reference data. Ten junior high school badminton players were invited to serve as the subjects of this study. They participated in a three-week (nine sessions) training program. Training was primarily in the form of fixed or random footwork drills. Timed tests were performed before and after each session to measure the players’ agility in performing six-point and four-point footwork drills. The results were compared to the training effects calculated using dependent-sample t-tests. In addition, the long-term durability and functionality of the training system were tested. The training system was able to maintain stable and reliable training and evaluation operations for extended periods. Results showed significant improvements in the visual reaction time (p = 0.003) and agility (p = 0.001) of players. The proposed training system is an affordable option for training and monitoring, evaluating, and recording training performance. It can accurately record movement and response times and simulate competitive environments.
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- 2020
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17. Perceptions on gender awareness and considerations in career choices of medical students in a medical school in Taiwan
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Yueh-Chin Chung, Ching-Yi Lin, Chien-Ning Huang, and Jen-Hung Yang
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Career choices ,Gender ,Medical curricula ,Medical students ,Medicine (General) ,R5-920 - Abstract
The trend of medical career choice in the younger generation has resulted in deficiency of manpower in the four major disciplines of internal medicine, surgery, obstetrics/gynecology, and pediatrics, which will threaten people's health care in Taiwan. However, perceptions of gender awareness and factors affecting the career choices of medical students have not been investigated systemically in Taiwan. To explore the perceptions on gender awareness and considerations in career choices, we recruited 280 1st- and 7th-year male and female medical students at a Medical University for the study. A modified Nijmegen questionnaire using a 5-point Likert scale containing medical curricula (18 items), gender awareness (13 items), and career inclination (9 items) was adopted as the investigation tool in our study. The response rate was 75% (224/280). With regard to gender, the 1st-year male students had greater confidence in being a physician than the female students (p
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- 2013
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18. Prediction of Newborn Birth Weight Based on the Estimation at 20–24 Weeks of Gestation
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Chi-Feng Su, Horng-Jyh Tsai, Ching-Yi Lin, Tsung-Ho Ying, Po-Hui Wang, and Gin-Den Chen
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birth weight estimation ,fetal biometrics ,maternal body mass index ,pregnancy interval ,ultrasound ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: The aim of this study was to develop a mathematical equation to predict the birth weight during the second trimester at 20-24 weeks of gestation. Materials and Methods: In a university hospital, 110 healthy pregnant women were eligible for inclusion at 20-24 weeks of gestation. We recorded the maternal weight (pre-pregnancy, mid-pregnancy, and at delivery) and body mass index (BMI), newborn birth weight, time period from ultrasound examination to term delivery, and also the fetal biometrics sonographically at 20-24 weeks of gestation. Pearson's correlation was used to verify the extent of the relationship between all the above measurements and the newborn birth weight. Multiple regressions with the stepwise method were used to analyze maternal weight factors, fetal biometrical factors, and pregnancy interval. An equation for term birth weight estimation during the second trimester was determined. Results: Maternal BMI at mid-pregnancy, time interval from mid-pregnancy to term, and abdominal circumference had the highest correlation with newborn birth weight (r = 0.388, 0.341, and 0.315, respectively, p < 0.05). Using the stepwise regression analysis, an optimal formula with variance of 0.303 was derived: estimated birth weight = −700 + 49.766 × (mid-pregnancy BMI [kg/m2]) + 13.362 × (time interval from mid-pregnancy to term delivery [days]) + 68.696 × (abdominal circumference [cm]). Conclusion: We propose an accurate, simple, and easy formula to better assess the newborn birth weight at mid-pregnancy for the Asian population. Mid-pregnancy BMI was a more significant factor for birth weight estimation than other maternal weight factors in this study.
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- 2010
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19. Praeruptorin A Inhibits Human Cervical Cancer Cell Growth and Invasion by Suppressing MMP-2 Expression and ERK1/2 Signaling
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Min-Hua Wu, Chia-Liang Lin, Hui-Ling Chiou, Shun-Fa Yang, Ching-Yi Lin, Chung-Jung Liu, and Yi-Hsien Hsieh
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Praeruptorin A ,cervical cancer cells ,ERK1/2 ,invasion ,MMP-2 ,TIMP-2 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Praeruptorin A (PA) is a pyranocumarin present in the dried root of Peucedanumpraeruptorum Dunn that has anticancer effects against several types of cells. However, the effect of PA on human cervical cancer cells is unknown. Our results indicate that PA significantly inhibited cell proliferation, colony formation, migration, invasion, and wound closure of HeLa and SiHa cells, induced cell cycle arrest at G0/G1 phase, upregulated Rb, p16, p21 and p27 proteins and downregulated cyclin D1 and S-phase kinase-associated protein 2 (Skp2) proteins. PA also significantly reduced expression of matrix metalloproteinase-2 (MMP-2) and increased expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). In addition, PA suppressed ERK1/2 activation and increased the effect of PD98059 (a specific MEK1/2 inhibitor) in downregulation of MMP-2 and upregulation of TIMP-2. PA treatment inhibited the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on upregulation of ERK1/2 activation, MMP-2 expression, cellular migration, and invasion of HeLa cells. Our findings are the first to demonstrate the activity of PA against cervical cancer cells, and suggest this agent has promise as a therapeutic agent in treatment of human cervical cancer.
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- 2017
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20. Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice.
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Marc A DePaul, Ching-Yi Lin, Jerry Silver, and Yu-Shang Lee
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Medicine ,Science - Abstract
The loss of lower urinary tract (LUT) control is a ubiquitous consequence of a complete spinal cord injury, attributed to a lack of regeneration of supraspinal pathways controlling the bladder. Previous work in our lab has utilized a combinatorial therapy of peripheral nerve autografts (PNG), acidic fibroblast growth factor (aFGF), and chondroitinase ABC (ChABC) to treat a complete T8 spinal cord transection in the adult rat, resulting in supraspinal control of bladder function. In the present study we extended these findings by examining the use of the combinatorial PNG+aFGF+ChABC treatment in a T8 transected mouse model, which more closely models human urinary deficits following spinal cord injury. Cystometry analysis and external urethral sphincter electromyograms reveal that treatment with PNG+aFGF+ChABC reduced bladder weight, improved bladder and external urethral sphincter histology, and significantly enhanced LUT function, resulting in more efficient voiding. Treated mice's injured spinal cord also showed a reduction in collagen scaring, and regeneration of serotonergic and tyrosine hydroxylase-positive axons across the lesion and into the distal spinal cord. Regeneration of serotonin axons correlated with LUT recovery. These results suggest that our mouse model of LUT dysfunction recapitulates the results found in the rat model and may be used to further investigate genetic contributions to regeneration failure.
- Published
- 2015
- Full Text
- View/download PDF
21. Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury.
- Author
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Keun Woo Park, Ching-Yi Lin, Kevin Li, and Yu-Shang Lee
- Subjects
Medicine ,Science - Abstract
Suppressors of cytokine signaling-3 (SOCS3) is associated with limitations of nerve growth capacity after injury to the central nervous system. Although genetic manipulations of SOCS3 can enhance axonal regeneration after optic injury, the role of SOCS3 in dendritic outgrowth after spinal cord injury (SCI) is still unclear. The present study investigated the endogenous expression of SOCS3 and its role in regulating neurite outgrowth in vitro. Interleukin-6 (IL-6) induces SOCS3 expression at the mRNA and protein levels in neuroscreen-1 (NS-1) cells. In parallel to SOCS3 expression, IL-6 induced tyrosine phosphorylation of signal transducer and activator of transcription 3 (STAT3) in NS-1 cells. Lentiviral delivery of short hairpin RNA (shSOCS3) (Lenti-shSOCS3) to decrease SOCS3 expression into NS-1 cells enhanced IL-6-induced tyrosine phosphorylation of STAT3 (P-STAT3 Tyr705) and promoted neurite outgrowth. In addition, we determined if reduction of SOCS3 expression by microinjection of Lenti-shSOCS3 into spinal cord enhances dendrite outgrowth in spinal cord neurons after SCI. Knocking down of SOCS3 in spinal cord neurons with Lenti-shSOCS3 increased complete SCI-induced P-STAT3 Tyr705. Immunohistochemical analysis showed that complete SCI induced a significant reduction of microtubule association protein 2-positive (MAP-2+) dendrites in the gray and white matter at 1 and 4 weeks after injury. The SCI-induced reduction of MAP-2+ dendrites was inhibited by infection with Lenti-shSOCS3 in areas both rostral and caudal to the lesion at 1 and 4 weeks after complete SCI. Furthermore, shSOCS3 treatment enhanced up-regulation of growth associated protein-43 (GAP-43) expression, which co-localized with MAP-2+ dendrites in white matter and with MAP-2+ cell bodies in gray matter, indicating Lenti-shSOCS3 may induce dendritic regeneration after SCI. Moreover, we demonstrated that Lenti-shSOCS3 decreased SCI-induced demyelination in white matter of spinal cord both rostral and caudal to the injury site 1 week post-injury, but not rostral to the injury at 4 weeks post-injury. Importantly, similar effects as Lenti-shSOCS3 on increasing MAP-2+ intensity and dendrite length, and preventing demyelination were observed when a second shSOCS3 (Lenti-shSOCS3 #2) was applied to rule out the possibilities of off target effects of shRNA. Collectively, these results suggest that knocking down of SOCS3 enhances dendritic regeneration and prevents demyelination after SCI.
- Published
- 2015
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- View/download PDF
22. Sex steroid hormone levels and reproductive development of eight-year-old children following in utero and environmental exposure to phthalates.
- Author
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Pen-Hua Su, Jia-Yuh Chen, Ching-Yi Lin, Hsiao-Yen Chen, Pao-Chi Liao, Tsung-Ho Ying, and Shu-Li Wang
- Subjects
Medicine ,Science - Abstract
In utero exposure to phthalates may adversely affect reproductive development in children due to the anti-androgenic properties of the pthalates. Accordingly, we aimed to determine the effects of in utero and environmental phthalate exposure on the reproductive development of eight-year-old children. We recruited 180 children in central Taiwan during November 2001 and followed them until August 2009 when all children became eight years old. Birth outcomes were collected. Bone age, hormone concentrations, and reproductive developmental stages were determined. Phthalate metabolite levels, including mono-2-ethylhexyl phthalate [MEHP], mono-n-butyl phthalate [MnBP], and mono-benzyl phthalate [MBzP], were assessed. No significant gender differences were found in in utero phthalate exposure. Maternal urinary levels of phthalate metabolites did not correlate significantly with birth outcomes, physical characteristics, and reproductive hormones of the eight-year-old children. Regarding the urinary phthalate metabolite levels of the eight-year-old children, MEHP correlated significantly with serum progesterone levels. MEHP levels in girls correlated significantly with serum progesterone levels. MnBP correlated significantly with serum FSH in all children. In girls, MnBP correlated with serum FSH, and MBzP correlated with serum progesterone and FSH levels. Urinary phthalate metabolite levels did not correlate with female developmental stages or the development of female reproductive organs. Phthalate metabolites did not correlate with the physical characteristics and reproductive hormones in boys. Therefore, environmental exposure to phthalates, as determined by urinary phthalate metabolite levels of eight-year-old children, may affect reproductive hormone levels in children, indicating that further studies on the environmental health effects of phthalates are warranted.
- Published
- 2014
- Full Text
- View/download PDF
23. Transabdominal Ultrasound-guided Intra-gestational Sac Single Dose MTX Injection in a Tubal Pregnancy
- Author
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Ching-Yi Lin and Yang-Tse Shih
- Subjects
Gynecology and obstetrics ,RG1-991 - Published
- 2010
- Full Text
- View/download PDF
24. Enhanced Memory Corruption Detection in C/C++ Programs.
- Author
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Ching-Yi Lin and Wuu Yang
- Published
- 2023
- Full Text
- View/download PDF
25. Non-Linear CNN-Based Read Channel for Hard Disk Drive With 30% Error Rate Reduction and Sequential 200-Mbits/s Throughput in 28-nm CMOS.
- Author
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Yuwei Qin, Ruben Purdy, Alec Probst, Ching-Yi Lin, and Jian-Gang Jimmy Zhu
- Published
- 2023
- Full Text
- View/download PDF
26. Tracking the Effects of Tumor Treating Fields on Human Breast Cancer Cells in vitro Using a Capacitance Sensing Lab-on-CMOS Microsystem.
- Author
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Yann Gilpin, Ching-Yi Lin, Mats Forssell, Siyang Zheng, Pulkit Grover, and Marc Dandin
- Published
- 2022
- Full Text
- View/download PDF
27. Non-linear CNN-based Read Channel for Hard Disk Drive with 30% Error Rate Reduction and Sequential 200Mbits/second Throughput in 28nm CMOS.
- Author
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Yuwei Qin, Ruben Purdy, Alec Probst, Ching-Yi Lin, and Jian-Gang Jimmy Zhu
- Published
- 2022
- Full Text
- View/download PDF
28. Upregulated 5-HT1A Receptors Regulate Lower Urinary Tract Function in Rats after Complete Spinal Cord Injury
- Author
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Ching-Yi Lin, Kevin Li, Rajaa Thalluri, and Yu-Shang Lee
- Subjects
Neurology (clinical) - Published
- 2023
29. Incidence of Sinus Membrane Perforation Using Two Types of Implant Drills: An Ex Vivo Animal Study.
- Author
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Ching-Yi Lin, Jerry, Wei Jen Chang, Nevins, Myron, and Kim, David M.
- Subjects
MAXILLARY sinus surgery ,DENTAL implants ,DENTAL equipment ,BIOLOGICAL models ,GOATS ,MAXILLARY sinus ,OSTEOTOMY ,ANIMALS - Abstract
This ex vivo study evaluates the incidence of sinus membrane perforation during implant site osteotomy with two different types of drills and drilling techniques. Fifty goat heads with 50 sinus pairs (100 sinus sides) were assigned to two groups (osseodensification bur [OB] group and inverse conical shape bur [ICSB] group) to simulate transcrestal sinus elevation (50 sinus sides per group). An osteotomy was performed to pass through the lateral sinus wall no more than 3 mm. The integrity of the sinus membranes was examined and confirmed under a microscope. Of the 50 sinuses per group, the OB group presented with 14 (28%) perforated sinuses, while the ICSB group presented with 2 (4%) perforated sinuses. Of the 14 perforations from the OB group, 6 (42.9%) showed a pinpoint perforation pattern, 4 (28.5%) of which were not visible until direct air pressure was applied. Overall, the ICSB drill group demonstrated a lower sinus perforation rate than the OB group. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
30. Effect of share collateralization by directors/supervisors on control rights, risk, and performance: application to the Taiwan market.
- Author
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Kuo-Jung Lee, Su-Lien Lu, and Ching-Yi Lin
- Published
- 2019
- Full Text
- View/download PDF
31. ASIC Implementation of Nonlinear CNN-Based Data Detector for TDMR System in 28 nm CMOS at 200 Mbits/s Throughput
- Author
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Yuwei Qin, Ruben Purdy, Alec Probst, Ching-Yi Lin, and Jian-Gang Zhu
- Subjects
Electrical and Electronic Engineering ,Electronic, Optical and Magnetic Materials - Published
- 2023
32. Graphical Tools for Doctor-Patient Communication: An App Prototype Design in Children's Pain Management.
- Author
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Fang Suey Lin, Ching-Yi Lin, Yu Jie Hsueh, Chun-Yi Lee, and Chun-Pei Hsieh
- Published
- 2016
- Full Text
- View/download PDF
33. User perception, intention, and attitude on mobile advertising.
- Author
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Ching-Wen Lin, Yen-Chung Hsu, and Ching-Yi Lin
- Published
- 2017
- Full Text
- View/download PDF
34. A New Method for Self-Estimation of the Severity of Obstructive Sleep Apnea Using Easily Available Measurements and Neural Fuzzy Evaluation System.
- Author
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Ming-Feng Wu, Wei-Chang Huang, Chia-Feng Juang, Kai-Ming Chang, Chih-Yu Wen, Yu-Hsuan Chen, Ching-Yi Lin, Yi-Chan Chen, and Ching-Cheng Lin
- Published
- 2017
- Full Text
- View/download PDF
35. An Intelligent Saliva Recognition System for Women's Ovulation Detection.
- Author
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Hui-Ching Wu, Ching-Yi Lin, Shing-Han Huang, and Ming-Hseng Tseng
- Published
- 2015
- Full Text
- View/download PDF
36. Measuring and Modeling Macrophage Growth using a Lab-on-CMOS Capacitance Sensing Microsystem
- Author
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Kyle Smith, Ching-Yi Lin, Yann Gilpin, Elizabeth Wayne, and Marc Dandin
- Subjects
Article - Abstract
We report on the use of a lab-on-CMOS biosensor platform for quantitatively tracking the growth of RAW 264.7 murine Balb/c macrophages. We show that macrophage growth over a wide sensing area correlates linearly with an average capacitance growth factor resulting from capacitance measurements at a plurality of electrodes dispersed in the sensing area. We further show a temporal model that captures the cell evolution in the area of interest over long periods (e.g., 30 hours). The model links the cell numbers and the average capacitance growth factor associated with the sensing area to describe the observed growth kinetics.
- Published
- 2023
37. CMOS Bioelectronics: Current and Future Trends
- Author
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Ching-Yi Lin, Md. Sakibur Sajal, Yann Gilpin, Fahimeh Dehghandehnavi, Anna Batueva, Kai-Chun Lin, Nicole McFarlane, and Marc Dandin
- Published
- 2022
38. Concept Shift Detection for Frequent Itemsets from Sliding Windows over Data Streams.
- Author
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Jia-Ling Koh and Ching-Yi Lin
- Published
- 2009
- Full Text
- View/download PDF
39. Automatic Visual Feature Extraction for Mandarin Audio-Visual Speech Recognition.
- Author
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Tsang-Long Pao, Wen-Yuan Liao, Tsan-Nung Wu, and Ching-Yi Lin
- Published
- 2009
- Full Text
- View/download PDF
40. Insights into deuterostome evolution from the biphasic transcriptional programmes of hemichordates
- Author
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Alberto Perez-Posada, Che-Yi Lin, Ching-Yi Lin, Yi-Chih Chen, José Luis Gómez Skarmeta, Jr-Kai Yu, Yi-Hsien Su, and Juan J. Tena
- Abstract
Evolutionary history of deuterostomes has remained unsolved and is intimately related to how chordates originated. Within the clade of deuterostomes, hemichordates and echinoderms (together called Ambulacraria) are sister groups of chordates; comparative studies among the three are insightful for understanding deuterostome evolution. Indirect-developing hemichordates, such as Ptychodera flava, develop into planktonic larvae, which are morphologically similar to echinoderm larvae, before metamorphosing into the adult body plan that retains an anteroposterior polarity homologous to that of chordates. Therefore, deciphering developmental programmes of indirect-developing hemichordates may provide a key for understanding the evolution of deuterostomes and chordate origins. Here, we characterise transcriptomes and chromatin accessibility across multiple developmental stages of P. flava and uncover biphasic developmental programmes controlled by different sets of transcription factors and their corresponding cis-regulatory elements. Transcriptome age and network analyses reveal that the gastrula transcriptome is relatively ancient and highly connected. By comparing developmental transcriptomes of hemichordate, sea urchin and amphioxus, a high conservation of gene expression during gastrulation is identified and extended to neurula stages of amphioxus, in addition to the highly similar larval transcriptomes among the three species. Moreover, P. flava possesses conserved interactions of transcription factors required for the development of echinoderm endomesoderm and chordate axial mesoderm. These results suggest a deuterostome phylotypic stage during gastrulation (corresponding to amphioxus gastrulation and neurulation) controlled by gene regulatory networks with conserved cis-regulatory interactions, and support the hypothesis that the deuterostome ancestor is an indirect-developer.
- Published
- 2022
41. Financial development and natural resources: The dynamics of China’s outward FDI
- Author
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Ling Feng, Lulan Ge, Zhiyuan Li, and Ching-Yi Lin
- Subjects
Economics and Econometrics ,Accounting ,Political Science and International Relations ,Economics ,International economics ,Foreign direct investment ,Financial development ,China ,Natural resource ,Finance - Published
- 2021
42. Trading signals analysis of mobile trading services.
- Author
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Ching-Wen Lin, Ching-Yi Lin, and Binshan Lin
- Published
- 2013
- Full Text
- View/download PDF
43. Segment-based emotion recognition from continuous Mandarin Chinese speech.
- Author
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Jun-Heng Yeh, Tsang-Long Pao, Ching-Yi Lin, Yao-Wei Tsai, and Yu-Te Chen
- Published
- 2011
- Full Text
- View/download PDF
44. Changes in drowning mortality rates and quality of reporting from 2004–2005 to 2014–2015: a comparative study of 61 countries
- Author
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Liang Yi Wang, Tsung Hsueh Lu, and Ching Yi Lin
- Subjects
Adult ,medicine.medical_specialty ,Adolescent ,Databases, Factual ,International comparisons ,030231 tropical medicine ,Global Health ,World Health Organization ,World health ,Death Certificates ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Epidemiology ,medicine ,Humans ,030212 general & internal medicine ,Mortality ,Child ,Aged ,Drowning ,business.industry ,Public health ,Mortality rate ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,Infant ,lcsh:RA1-1270 ,Middle Aged ,Death certificate ,Mortality data ,Child, Preschool ,Accidents ,Biostatistics ,business ,Demography ,Research Article - Abstract
Background This study assessed international variations in changes in drowning mortality rates and the quality of reporting specific information in death certificates over the past decade. Methods Drowning mortality data of 61 countries were extracted from the World Health Organization Mortality Database. We calculated the percentage change (PC) in age-standardized drowning mortality rates and percentage of drowning deaths reported with unspecified codes between 2004 and 2005 and 2014–2015. Results Of the 61 countries studied, 50 exhibited a reduction in drowning mortality rates from 2004 to 2005 to 2014–2015. Additionally, five countries—Lithuania, Moldova, Kyrgyzstan, Romania, and El Salvador—with a high mortality rate in 2004–2005 (> 40 deaths per 100,000) showed improvement (PC 40%) exhibited a marked reduction (PC Conclusions Large international variations in the extent of changes in drowning mortality rates and the quality of reporting specific information on the death certificate were observed during the study period.
- Published
- 2019
45. RFID tags: privacy and security aspects.
- Author
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Dong-Her Shih, Ching-Yi Lin, and Binshan Lin
- Published
- 2005
- Full Text
- View/download PDF
46. Norcantharidin combined with paclitaxel induces endoplasmic reticulum stress mediated apoptotic effect in prostate cancer cells by targeting SIRT7 expression
- Author
-
Su-Chun Hui, Hui-Ling Chiou, Chien-Hsing Lee, Ching-Yi Lin, Yong-Syuan Chen, Yi-Hsien Hsieh, and Min-Hua Wu
- Subjects
Male ,Programmed cell death ,Norcantharidin ,Paclitaxel ,Cell growth ,Chemistry ,Health, Toxicology and Mutagenesis ,Prostatic Neoplasms ,General Medicine ,Management, Monitoring, Policy and Law ,Cell cycle ,Toxicology ,Bridged Bicyclo Compounds, Heterocyclic ,Endoplasmic Reticulum Stress ,chemistry.chemical_compound ,DU145 ,Apoptosis ,Cell Line, Tumor ,Unfolded protein response ,Cancer research ,Humans ,Sirtuins ,Viability assay - Abstract
Prostate cancer (PCa), an extremely common malignancy in males, is the most prevalent disease in several countries. Norcantharidin (NCTD) has antiproliferation, antimetastasis, apoptosis, and autophagy effects in various tumor cells. Nevertheless, the antitumor effect of NCTD combined with paclitaxel (PTX), a chemotherapeutic drug, in PCa remains unknown. The cell growth, proliferative rate, cell cycle distribution, and cell death were determined by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide, colony formation assay, PI staining, and Annexin V/PI staining by flow cytomertry, whereas the mitochondrial membrane potential (MMP) and endoplasmic reticulum (ER) stress was evaluated using the MitoPotential assay and ER-ID red assay. We also evaluated the protein and mRNA expression of SIRTs by Western blotting and qRTPCR assay. Overexpression effectivity was measured by DNA transfection assay. Our study showed that cell viability and proliferative PC3 and DU145 rates were effectively inhibited after NCTD-PTX combination. We also found that NCTD-PTX combination treatment significantly enhance G2/M phase arrest, induction of cell death and ER stress, loss of MMP, and ER- or apoptotic-related protein expression. Furthermore, NCTD-PTX combination treatment was significantly decreasing the protein and mRNA expression of SIRT7 in PCa cells. Combination therapy effectively reduced cell viability, ER stress-mediated apoptosis and p-eIF2α/ATF4/CHOP/cleaved-PARP expression inhibition in SIRT7 overexpression of PCa cells. These results indicate that NCTD combined with PTX induces ER stress-mediated apoptosis of PCa cells by regulating the SIRT7 expression axis. Moreover, combination therapy may become a potential therapeutic strategy against human PCa.
- Published
- 2021
47. Mitochondrial haplogroups have a better correlation to insulin requirement than nuclear genetic variants for type 2 diabetes mellitus in Taiwanese individuals
- Author
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Tsu-Kung Lin, Jiin-Haur Chuang, Meng-Han Tsai, Sung-Chou Li, Pei-Wen Wang, Ching-Yi Lin, Chia-Wei Liou, Yu-Jih Su, Shun-Jen Chang, Jung-Fu Chen, Feng-Chih Shen, Shao-Wen Weng, and Yen-Hsiang Chang
- Subjects
Male ,Mitochondrial DNA ,Endocrinology, Diabetes and Metabolism ,Taiwan ,Single-nucleotide polymorphism ,Type 2 diabetes ,DNA, Mitochondrial ,Polymorphism, Single Nucleotide ,Haplogroup ,Diseases of the endocrine glands. Clinical endocrinology ,Insulin resistance ,Asian People ,Internal Medicine ,medicine ,Humans ,Hypoglycemic Agents ,Insulin ,Genetic Predisposition to Disease ,Genetics ,business.industry ,Haplotype ,Diabetes ,Type 2 Diabetes Mellitus ,High-Throughput Nucleotide Sequencing ,General Medicine ,Articles ,Middle Aged ,medicine.disease ,RC648-665 ,Mitochondria ,Clinical Science and Care ,Diabetes Mellitus, Type 2 ,Haplotypes ,Female ,Original Article ,Insulin Resistance ,business ,Human mitochondrial DNA haplogroup - Abstract
Aims/Introduction Identifying diabetes‐susceptible genetic variants will help to provide personalized therapy for the management of type 2 diabetes. Previous studies have reported a genetic risk score (GRS), computed by the sum of nuclear DNA (nDNA) risk alleles, that may predict the future requirement for insulin therapy. Although mitochondrial dysfunction has a close association with insulin resistance (IR), there are few studies investigating whether genetic variants of mitochondrial DNA (mtDNA) will affect the clinical characteristics of type 2 diabetes. Materials and Methods Mitochondrial haplogroups were determined using mtDNA whole genome next generation sequencing and 13 single nucleotide polymorphisms (SNPs) in nDNA susceptibility loci of 13 genes in 604 Taiwanese subjects with type 2 diabetes. A GRS of nDNA was computed by summation of the number of risk alleles. The correlation between the mtDNA haplogroup and the clinical characteristics of type 2 diabetes was assessed by logistic regression analysis. The results were compared with the GRS subgroups for the risk of insulin requirement. Results Mitochondrial haplogroups modulate the clinical characteristics of type 2 diabetes, in which patients harboring haplogroup D4, compared with those harboring non‐D4 haplotypes, were less prone to require insulin treatment, after adjusting for age, gender, and diabetes duration. However, there was no association between insulin requirement and GRS calculated from nuclear genetic variants. Conclusions Mitochondrial haplogroups, but not nuclear genetic variants, have a better association with the insulin requirement. The results highlight the role of mitochondria in the management of common metabolic diseases., Mitochondrial haplogroups, but not nuclear genetic variants, have a better association with the insulin requirement in patients with T2DM.
- Published
- 2021
48. Distribution of Risso's dolphin (Grampus griseus) in the east-central coastal waters of Taiwan based on whale-watching records
- Author
-
Hsin-Yi Yu, Ching-Yi Lin, Wen-Jyh Yu, and Lien-Siang Chou
- Subjects
taiwan ,grampus griseus ,group composition ,lcsh:Biology (General) ,citizen scientist ,distribution ,risso's dolphin ,lcsh:QH301-705.5 ,group size - Abstract
Risso’s dolphin occurs in tropical and temperate waters worldwide, and is one of the most common cetacean species in Taiwanese waters. This study attempted to reveal its distribution in water-depth ranges and variations in group size and composition (with or without mother-calf pairs). Based on sighting records from experienced whale-watching guides on board whale-watching boats operated in eastern Taiwan, 1630 sightings of Risso’s dolphin were recorded from 3939 touring trips near Hualien County from 1998 to 2014. Comparing the frequency distribution of water depths between ship tracks and dolphin sightings, we found that Risso’s dolphin commonly occurred on the continental shelf and slope, and mainly stayed in waters of 300~1500 m in depth, with a mean of 862.1 ± 12.3 m (standard error). The frequency distribution of water depths showed a significant difference between sites (deeper in the Shirti area than in the Hualien area), but no significant difference was detected among various group sizes. Nevertheless, the spatial distribution significantly varied with group composition, but not with group size. Groups with mother-calf pairs stayed in significantly shallower water, which was proposed to be related to predator avoidance. In addition, the large fluctuation in annual sighting ratios of Risso’s dolphin that ranged 0.1~0.7 sightings per trip during the 17-year period may be related to variations in prey abundances, the confirmation of which requires future investigation.
- Published
- 2019
49. Changes in the number and causes of maternal deaths after the introduction of pregnancy checkbox on the death certificate in Taiwan
- Author
-
Meng Chih Lee, Ching-Yi Lin, Liang-Yi Wang, Pao Lin Kuo, Pei Yin Tsai, Gin-Den Chen, and Tsung-Hueh Lu
- Subjects
Adult ,medicine.medical_specialty ,Taiwan ,Checkbox ,lcsh:Gynecology and obstetrics ,Death Certificates ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Cause of Death ,medicine ,Humans ,lcsh:RG1-991 ,Cause of death ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics ,Obstetrics and Gynecology ,medicine.disease ,Time of death ,Pregnancy Complications ,Maternal Mortality ,Mortality data ,Maternal Death ,Female ,Death certificate ,business - Abstract
Objective: To examine changes in the number and causes of maternal deaths after the introduction of pregnancy checkbox on the death certificate in January 2014 in Taiwan. Materials and methods: We first used the cause-of-death (COD) mortality data for years 2010 through 2017 to examine the number of deaths by item of pregnancy checkbox. We then compared the distribution of the causes of maternal deaths before and after the introduction of pregnancy checkbox. Results: Between 2014 and 2017, 111 women died, for whom the certifiers indicated the following in the pregnancy checkbox items: 2 (pregnant at the time of death; n = 10), 3 (died within 42 days after the termination of pregnancy; n = 64), and 4 (died between 43 days and 1 year after the termination of pregnancy; n = 37). However, in only 61 of the 111 deaths, the certifiers reported pregnancy or delivery-related diagnosis in the COD section of the death certificate—5 each for items 2 and 4 and 51 for item 3. The number of maternal deaths was 55 in 2010–2013; this number increased to 82 in 2014–2017. A decline in the percentage of maternal deaths from obstetric hemorrhage was noted from 38% (21/55) in 2010–2013 to 21% (17/82) in 2014–2017. Conclusion: The number of maternal deaths increased, and the distribution of causes of maternal deaths changed after the introduction of pregnancy checkbox. Additional studies are required to examine the possible misclassification of pregnancy-associated deaths indicated in the pregnancy checkbox. Keywords: Cause-of-death, Death certificate, Pregnancy checkbox, Pregnancy-associated deaths, Maternal deaths
- Published
- 2019
50. Employing GIS towards shaping a smart and sustainable future: a brief policy survey of global and Taiwan’s efforts
- Author
-
Yin-Ling Huang, Meng-Ying Li, Kun-Chi Lai, Chine-Hung Tung, Jr-Jie Jang, Hsiung-Ming Liao, Ching-Yi Lin, and Chih-Wei Chen
- Subjects
Sustainable development ,Government ,Geographic information system ,Process management ,Digital era ,business.industry ,media_common.quotation_subject ,Data management ,Atomic and Molecular Physics, and Optics ,Open data ,Promotion (rank) ,Business ,Electrical and Electronic Engineering ,Function (engineering) ,media_common - Abstract
Since UN announced 17 SDGs in 2015, many countries around the world have been endeavouring to promote SDGs towards building a sustainable future. Given the disparity of the regional development, national government is suggested to establish localised sustainable vision. Drawing on UN SDGs with targets and corresponding indicators, meanwhile considering local circumstances and sustainable vision, government further seeks to establish localised SDGs with related targets and indicators. Meanwhile, under the digital era, digital technologies have been extensively employed as the smart tool in many fields nowadays, and Geographic Information System (GIS) has been developed as the platform to visualise the SDGs progress in UN and many countries. On the above basis, this paper further demonstrates Taiwan’s efforts to establish localised SDGs, and develop National Geographic Information System (NGIS) to implement the sustainable development in Taiwan, monitor the SDGs progress, and provide feedback to policymakers to further make strategic policies in a top-down approach, meanwhile develop Community Geographic Information System (CGIS) to encourage stakeholders and citizens to harness the concept of CGIS to proactively create and tell their own stories and promote Regional Revitalisation policy in a bottom-up approach. Moreover, GIS could not function well without appropriate data management including massive data and open data policy, well-built digital infrastructure, as well as the selected “right data” and cyber security. Hence, with appropriate data management, GIS as a smart tool could facilitate the promotion and implementation of SDGs in an intuitive manner towards shaping a smart and sustainable future.
- Published
- 2019
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