87 results on '"Chiodi, E"'
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2. Ruolo attuale dell’imaging cardiaco nella diagnosi di endocardite infettiva [Role of cardiac imaging in the diagnosis of infective endocarditis]
- Author
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Mele, D., Flamigni, F., Cultrera, R., Cittanti, C., Chiodi, E., Fiorencis, A., and Ferrari, R.
- Subjects
Endocardite infettiva ,Positron emission tomography ,Echocardiography ,Computed tomography, Echocardiography, Infective endocarditis, Positron emission tomography ,Tomografia ad emissione di positroni ,Infective endocarditis ,Computed tomography ,Tomografia computerizzata ,NO ,Ecocardiografia, Endocardite infettiva, Tomografia ad emissione di positroni, Tomografia computerizzata ,Ecocardiografia - Published
- 2020
3. An informatic database to assist people with ID
- Author
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Albertini, G. and Chiodi, E.
- Published
- 2010
4. Layer-by-layer deposition of functional click polymers for microarray applications
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Sola, L., primary, Romanato, A., additional, Siboni, M. B., additional, Damin, F., additional, Chiodi, E., additional, Brambilla, D., additional, Cretich, M., additional, Gori, A., additional, and Chiari, M., additional
- Published
- 2019
- Full Text
- View/download PDF
5. Do Social Media Data predict changes in young adults’ employment status? Evidence from Italy
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Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, Bonanomi, Andrea, Sironi, Emiliano, Bonanomi Andrea (ORCID:0000-0003-2857-1430), Sironi Emiliano (ORCID:0000-0001-9129-3133), Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, Bonanomi, Andrea, Sironi, Emiliano, Bonanomi Andrea (ORCID:0000-0003-2857-1430), and Sironi Emiliano (ORCID:0000-0001-9129-3133)
- Abstract
This study addresses the conditions in which young Italian people find themselves during the active job search via Social Media Data. Focusing especially on NEETs, the aims of the study are: 1) to predict the changes in employment status from traditional information by using the longitudinal representative survey Rapporto Giovani; 2) to identify the target population inferring from their online digital traces; 3) to predict changes in employment status from social media data. We tried to predict the employment status transitions based on the digital behaviour using a new Facebook application, LikeYouth, that gathers information regarding Facebook profile and Likes on Facebook Pages of each user.
- Published
- 2018
6. Cambi di paradigma in tema di aorta: Implicazioni cliniche e terapeutiche - Imaging clinico nelle sindromi croniche ed acute. L'aorta come causa di malattia cardiaca
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Nistri S, Roghi A, Mele D, Chiodi E, Colombo E, d'Amati G, Angelini A, Basso C, Pepe G, Thiene G., BIAGINI, ELENA, LEONE, ORNELLA, RAPEZZI, CLAUDIO, Nistri S, Roghi A, Mele D, Biagini E, Chiodi E, Colombo E, d'Amati G, Leone O, Angelini A, Basso C, Pepe G, Rapezzi C, and Thiene G
- Subjects
Magnetic resonance imaging ,Echocardiography ,Aortic size ,Thoracic aortic disease ,Aortic function ,Computed tomography ,NO - Abstract
Multimodal imaging plays a pivotal role in the assessment of the thoracic aorta, both in chronic and acute settings. Moving from improved knowledge on the structure and function of the aortic wall, as well as on its pathophysiology and histopathology, appropriate utilization of each imaging modality results into a better definition of the patient's need and proper treatment strategy. This review is aimed at highlighting the most critical aspects in this field, providing cardiologists with some novel clues for the imaging approach to patients with thoracic aortic disease. © 2014 Il Pensiero Scientifico Editore.
- Published
- 2014
7. Italian registry of cardiac magnetic resonance
- Author
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Francone, M, Di Cesare, E, Cademartiri, F, Pontone, G, Lovato, L, Matta, G, Secchi, F, Maffei, E, Pradella, S, Carbone, I, Marano, R, Bacigalupo, L, Chiodi, E, Donato, R, Sbarbati, S, Di, Renzi, Cmr Italian Registry Group, Ligabue, G, Mancini, A, Palmierir, F, Restaino, G, Puppini, G, Centonze, M, Toscano, W, Tessa, C, Faletti, R, Conti, M, Scardapane, A, Galea, S, Liguori, C, Pagliacci, M, Lumia, D, Nullm, Nulldi Girolamo, Romagnoli, A, Guarise, A, Cirillo, S, Gagliardi, B, Borghi, C, Quarenghi, M, Contin, F, Scaranello, F, Tartaro, A, Marinucci, C, Monti, L., DE COBELLI, FRANCESCO, Francone, M, Di Cesare, E, Cademartiri, F, Pontone, G, Lovato, L, Matta, G, Secchi, F, Maffei, E, Pradella, S, Carbone, I, Marano, R, Bacigalupo, L, Chiodi, E, Donato, R, Sbarbati, S, DE COBELLI, Francesco, Di, Renzi, Cmr Italian Registry, Group, Ligabue, G, Mancini, A, Palmierir, F, Restaino, G, Puppini, G, Centonze, M, Toscano, W, Tessa, C, Faletti, R, Conti, M, Scardapane, A, Galea, S, Liguori, C, Pagliacci, M, Lumia, D, Nullm, Nulldi Girolamo, Romagnoli, A, Guarise, A, Cirillo, S, Gagliardi, B, Borghi, C, Quarenghi, M, Contin, F, Scaranello, F, Tartaro, A, Marinucci, C, and Monti, L.
- Subjects
Adult ,Male ,medicine.medical_specialty ,Referral ,Heart disease ,Adolescent ,Heart Diseases ,Clinical indications ,Cardiomyopathy ,Infarction ,Magnetic Resonance Imaging, Cine ,safety profile ,cardiac magnetic resonance ,CMR-registry ,Coronary artery disease ,Young Adult ,medicine ,Prevalence ,Humans ,Radiology, Nuclear Medicine and imaging ,Registries ,Young adult ,Practice Patterns, Physicians' ,Child ,acquisition protocols ,Settore MED/36 - DIAGNOSTICA PER IMMAGINI E RADIOTERAPIA ,Aged ,Aged, 80 and over ,business.industry ,Infant ,General Medicine ,Middle Aged ,medicine.disease ,patient's management ,clinical indications ,cmr-registry ,Italy ,Radiological weapon ,Child, Preschool ,Utilization Review ,Female ,Radiology ,Cardiac magnetic resonance ,business ,cardiomyopathy ,coronary artery disease - Abstract
Objectives Forty sites were involved in this multicenter and multivendor registry, which sought to evaluate indications, spectrum of protocols, impact on clinical decision making and safety profile of cardiac magnetic resonance (CMR). Materials and methods Data were prospectively collected on a 6-month period and included 3376 patients (47.2 ± 19 years; range 1–92 years). Recruited centers were asked to complete a preliminary general report followed by a single form/patient. Referral physicians were not required to exhibit any specific certificate of competency in CMR imaging. Results Exams were performed with 1.5 T scanners in 96% of cases followed by 3 T (3%) and 1 T (1%) magnets and contrast was administered in 84% of cases. The majority of cases were performed for the workup of inflammatory heart disease/cardiomyopathies representing overall 55.7% of exams followed by the assessment of myocardial viability and acute infarction (respectively 6.9% and 5.9% of patients). In 49% of cases the final diagnosis provided was considered relevant and with impact on patient's clinical/therapeutic management. Safety evaluation revealed 30 (0.88%) clinical events, most of which due to patient's preexisting conditions. Radiological reporting was recorded in 73% of exams. Conclusions CMR is performed in a large number of centers in Italy with relevant impact on clinical decision making and high safety profile.
- Published
- 2013
8. Extramedullary hematopoiesis is associated with lower cardiac iron loading in chronically transfused thalassemia patients
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Ricchi, P, Meloni, A, Spasiano, A, Neri, Mg, Gamberini, Mr, Cuccia, L, Caruso, V, Gerardi, C, D'Ascola, Dg, Rosso, R, Campisi, S, Rizzo, M, Terrazzino, F, Vangosa, Ab, Chiodi, E, Missere, M, Mangione, M, Positano, V, and Pepe, A
- Published
- 2015
9. [Paradigm shifts in aortic pathology: clinical and therapeutic implications. Clinical imaging in chronic and acute aortic syndromes. The aorta as a cause of cardiac disease]
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Nistri, S, Roghi, A, Mele, D, Biagini, E, Chiodi, E, Colombo, E, D'Amati, G, Leone, O, Angelini, Annalisa, Basso, Cristina, Pepe, G, Rapezzi, C, and Thiene, Gaetano
- Subjects
Aortic aneurysm ,Aortic Aneurysm, Thoracic ,Heart Diseases ,Syndrome ,Magnetic Resonance Imaging ,Sensitivity and Specificity ,Aortic Dissection ,Nuclear Medicine & Medical Imaging ,Pathology ,Thoracic aorta Cardiovascular System & Radiology ,Predictive Value of Tests ,Acute Disease ,Chronic Disease ,Humans ,Tomography, X-Ray Computed ,Echocardiography, Transesophageal - Abstract
Multimodal imaging plays a pivotal role in the assessment of the thoracic aorta, both in chronic and acute settings. Moving from improved knowledge on the structure and function of the aortic wall, as well as on its pathophysiology and histopathology, appropriate utilization of each imaging modality results into a better definition of the patient's need and proper treatment strategy. This review is aimed at highlighting the most critical aspects in this field, providing cardiologists with some novel clues for the imaging approach to patients with thoracic aortic disease.
- Published
- 2014
10. Movement abnormalities in the left ventricle of thalassemia major patients
- Author
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Meloni, A., Gulino, L., Casini, T., Sanna, P., Piraino, B., Smacchia, Maria Paola, Resta, M., Chiodi, E., Salvatori, C., Positano, V., Lombardi, M., and Pepe, A.
- Published
- 2013
11. Heart T2* for Prediction of Cardiac Complications in well-treated thalassemia major patients
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Pepe, A, Meloni, A, Rossi, G, Keilberg, P, Batzella, Mg, Carollo, A, Salvatori, C, Vallone, A, Chiodi, E, Valeri, G, and Lombardi, M
- Published
- 2012
12. Are the preferential patterns of myocardial iron overload preserved at the CMR follow-up?
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Meloni, A, Positano, V, De Marchi, D, Chiodi, E, Missere, M, Vallone, A, Midiri, M, Gulino, L, Renni, R, Lombardi, M, and Pepe, A
- Published
- 2012
13. Heart T2* assessment for iron overload measurement in thalassemia major patients using different software tools
- Author
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Chiodi, E, Positano, V, Furieri, E, Cavedagna, D, Carli, F, Meloni, A, M R, Gamberini, Borgna-Pignatti, C, and Pepe, A
- Published
- 2012
14. Feasibility, reproducibility and reliability for the T2* iron evaluation at 3T in comparison with 1.5T
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Tudisca, C, Meloni, A, Valeri, G, Chiodi, E, Missere, M, Carollo, A, Lombardi, M, Midiri, M, and Pepe, A
- Published
- 2012
15. Left Ventricular Volumes, Mass and Function normalized to the body surface area, age and gender from CMR in a large cohort of well-treated Thalassemia Major patients without myocardial iron overload
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Meloni, A, Aquaro, G, Festa, P, Gagliardotto, F, Zuccarelli, A, Gerardi, C, Santodirocco, M, M A, Romeo, M R, Gamberini, Chiodi, E, Positano, V, Lombardi, M, and Pepe, A
- Published
- 2011
16. Adrenal Incidentaloma in Thalassemia: A Case Report and Literature Review
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Gamberini, M. R., Prandini, N., Chiodi, E., Farneti, Carlotta, and Garani, MARIA CHIARA
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Incidentaloma ,Thalassaemia ,Myelolipoma - Published
- 2011
17. Onset of cardiac iron loading in a large and homogenous cohort of thalassemia major pediatric patients
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Pepe, A, Meloni, A, Piraino, B, Missere, M, Renne, S, Chiodi, E, Positano, V, De Marchi, D, Filosa, A, and Lombardi, M
- Published
- 2011
18. Ruolo delle metodiche di imaging cardiologico nella terapia di resincronizzazione cardiaca: position paper AIAC
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Mele, D, Sassone, B, Bisignani, G, Caso, P, Cascolo, G, Galderisi, M, Nej Peraldo, C, Porciani, C, Chiodi, E, Bonso, A, Di Cori, A, Ficili, S, and Ghio, S
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Socio-culturale - Published
- 2011
19. CardiacIron Loading Measured by Multislice Multiecho T2* MRI and Cardiac Disease In Male and Female Patients with Thalassemia Major
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Pepe, A, Marsella, M, Meloni, A, Caldarelli, V, M C, Dell’Amico, Chiodi, E, Valeri, G, Positano, V, De Franceschi, L, Lombardi, M, and Borgna-Pignatti, C
- Published
- 2010
20. I paesaggi vitivinicoli del Piemonte: un nuovo sito Unesco?
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Borlizzi, Patrizia and Chiodi, E.
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- 2010
21. Italian registry of cardiac magnetic resonance
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Francone, M, Di Cesare, E, Cademartiri, F, Pontone, G, Lovato, L, Matta, G, Secchi, F, Maffei, E, Pradella, S, Carbone, I, Marano, Riccardo, Bacigalupo, L, Chiodi, E, Donato, R, Sbarbati, S, De Cobelli, F, Di Renzi, P., Marano, Riccardo (ORCID:0000-0003-2710-2093), Francone, M, Di Cesare, E, Cademartiri, F, Pontone, G, Lovato, L, Matta, G, Secchi, F, Maffei, E, Pradella, S, Carbone, I, Marano, Riccardo, Bacigalupo, L, Chiodi, E, Donato, R, Sbarbati, S, De Cobelli, F, Di Renzi, P., and Marano, Riccardo (ORCID:0000-0003-2710-2093)
- Abstract
Forty sites were involved in this multicenter and multivendor registry, which sought to evaluate indications, spectrum of protocols, impact on clinical decision making and safety profile of cardiac magnetic resonance (CMR).
- Published
- 2014
22. Architetti e Ingegneri Militari in Piemonte tra ‘500 e ‘700
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VIGLINO DAVICO, M., Chiodi, E., Franchini, Caterina, and Perin, A.
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Amoretti Filippo ,Topografia ,Gallo Francesco ,Riccio Paolo ,D’Aponte G ,Bagetti Pietro Giuseppe ,Bertola Giuseppe Francesco Ignazio ,Ufficio di topografia reale ,Borgiotti Ignazio Costanzo ,Castellino Giuseppe ,Storia del paesaggio ,Isnardi Giovanni Maurizio ,Michaud Francesco ,Vergnasco Carlo Francesco ,Gavuzzi Ignazio ,Sottis Giovanni Battista ,Simondi Antonio ,Boetto Giovenale ,Gabaleone Casimiro di Salmour ,Carello Domenico ,Durieu Antonio ,Rossi Giovan Battista ,Storia dell'Architettura ,Cantù Giovanni Giacomo ,Franchini Vittorio ,Papacino D’Antoni Alessandro Vittori ,Formazione ingegneri militari ,De Paoli Paolo Francesco ,Boldrini Giovanni Andrea ,Luzzo Giovanni Battista ,Gianotti Gianbattista ,Bergalli L.T.P ,Vallino Giuseppe ,Marciot ,Scuole di Artiglieria ,Bertola Antonio ,Bertola Francesco Antonio ,Magaris Giovan Battista ,Malansena Vassallo ,Avico Giovan Giuseppe ,Tuchieri Giuseppe Michele ,Vachieri Marco ,Chiapasco Domenico ,Ogliani Giuseppe ,Marchetti Antonio ,Prunotto Giovanni Tommaso ,Celoniato Giovanni Battista ,Denisio Pietro Vincenzo ,Isnardi Giacomo Maria ,Buscaglione Carlo Antonio ,Audibert Pierre ,Audé Pietro Antonio ,Gianotti Luigi ,Occelli Paolo ,Gavuzzi Ferdinando Giuseppe Lorenzo ,De Margherita Giovanni Andrea ,Bosio Carlo ,Pinto di Barri Bernardino ,Momo Giuseppe ,Nicolis di Robilant Filippo Giambattista ,Ingegneri miliatari ,Fedele Felice ,Topografi ,Banzes Antonio ,Bozzolino Ignazio Andrea ,Cartografia militare ,Boldrini Giuseppe Maria ,Nicolis di Robilant Spirito Antonio Benedetto ,Boasso Vittorio ,Birago di Borgaro Ignazio Renato Camillo ,Zino Gerolamo Francesco ,Architetti militari ,Fortificazioni ,Amoretti Francesco ,Audé Giacomo ,Blavet Giovan Giuseppe Francesco ,Boveri Giuseppe Andrea ,Denisio Giuseppe Vincenzo ,Michelotti Francesco Domenico ,Quaglia Giovanni ,Rana Carlo Andrea ,Salassa Pietro - Published
- 2008
23. These abstracts have been selected for VIEWING only as ePosters and in print. ePosters will be available on Screen A & B throughout the meeting, Print Posters at the times indicated below. Please refer to the PROGRAM for more details.
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Secchi, F., primary, Cannao, P., additional, Pluchinotta, F., additional, Butera, G., additional, Carminati, M., additional, Sardanelli, F., additional, Lombardi, M., additional, Monney, P., additional, Piccini, D., additional, Rutz, T., additional, Vincenti, G., additional, Coppo, S., additional, Koestner, S., additional, Stuber, M., additional, Schwitter, J., additional, Romana, P., additional, Francesco, S., additional, Gianfranco, B., additional, Mario, C., additional, Massimo, L., additional, Alizadeh Sani, Z., additional, Vojdan-Parast, M., additional, Alimohammadi, M., additional, Sarafan-Sadeghi, S., additional, Seifi, A., additional, Fallahabadi, H., additional, Karami Tanha, F., additional, Jamshidi, M., additional, Hesamy, M., additional, Bonello, B., additional, Sorensen, C., additional, Fouilloux, V., additional, Gorincour, G., additional, Mace, L., additional, Fraisse, A., additional, Jacquier, A., additional, de Meester, C., additional, Amzulescu, M., additional, Bouzin, C., additional, Boileau, L., additional, Melchior, J., additional, Boulif, J., additional, Lazam, S., additional, Pasquet, A., additional, Vancrayenest, D., additional, Vanoverschelde, J., additional, Gerber, B., additional, Loudon, M., additional, Bull, S., additional, Bissell, M., additional, Joseph, J., additional, Neubauer, S., additional, Myerson, S., additional, Dorniak, K., additional, Hellmann, M., additional, Rawicz-Zegrzda, D., additional, W sierska, M., additional, Sabisz, A., additional, Szurowska, E., additional, Heiberg, E., additional, Dudziak, M., additional, Kwok, T., additional, Chin, C., additional, Dweck, M., additional, Hadamitzky, M., additional, Nadjiri, J., additional, Hendrich, E., additional, Pankalla, C., additional, Will, A., additional, Schunkert, H., additional, Martinoff, S., additional, Sonne, C., additional, Pepe, A., additional, Meloni, A., additional, Terrazzino, F., additional, Spasiano, A., additional, Filosa, A., additional, Bitti, P., additional, Tangari, C., additional, Restaino, G., additional, Resta, M., additional, Ricchi, P., additional, Tudisca, C., additional, Grassedonio, E., additional, Positano, V., additional, Piraino, B., additional, Romano, N., additional, Keilberg, P., additional, Midiri, M., additional, Macchi, S., additional, Ambrosio, D., additional, De Marchi, D., additional, Chiodi, E., additional, Salvatori, C., additional, Artang, R., additional, Bogachkov, A., additional, Botelho, M., additional, Bou-Ayache, J., additional, Vazquez, M., additional, Carr, J., additional, Collins, J., additional, Maret, E., additional, Ahlander, B., additional, Bjorklund, P., additional, Engvall, J., additional, Cimermancic, R., additional, Inage, A., additional, Mizuno, N., additional, Santarelli, M., additional, Izzi, G., additional, Maddaloni, D., additional, Landini, L., additional, Carulli, G., additional, Oliva, E., additional, Arcioni, F., additional, Fraticelli, V., additional, Toia, P., additional, Renne, S., additional, Rizzo, M., additional, Reinstadler, S., additional, Klug, G., additional, Feistritzer, H., additional, Aschauer, A., additional, Schocke, M., additional, Franz, W., additional, Metzler, B., additional, Melonil, A., additional, Positanol, V., additional, Roccamo, G., additional, Argento, C., additional, Benni, M., additional, De Marchil, D., additional, Missere, M., additional, Prezios, P., additional, Salvatoril, C., additional, Pepel, A., additional, Rossi, G., additional, Cirotto, C., additional, Filati, G., additional, Preziosi, P., additional, Mongeon, F., additional, Fischer, K., additional, Teixeira, T., additional, Friedrich, M., additional, Marcotte, F., additional, Zenge, M., additional, Schmidt, M., additional, Nadar, M., additional, Chevre, P., additional, Rohner, C., additional, Mouratoglou, S., additional, Kallifatidis, A., additional, Giannakoulas, G., additional, Grapsa, J., additional, Kamperidis, V., additional, Pitsiou, G., additional, Stanopoulos, I., additional, Hadjimiltiades, S., additional, Karvounis, H., additional, Ahmed, N., additional, Lawton, C., additional, Ghosh Dastidar, A., additional, Frontera, A., additional, Jackson, A., additional, Cripps, T., additional, Diab, I., additional, Duncan, E., additional, Thomas, G., additional, Bucciarelli-Ducci, C., additional, Kannoly, S., additional, Gosling, O., additional, Ninan, T., additional, Fulford, J., additional, Dalrymple-Haym, M., additional, Shore, A., additional, Bellenger, N., additional, Alegret, J., additional, Beltran, R., additional, Martin, M., additional, Mendoza, M., additional, Elisabetta, C., additional, Teresa, C., additional, Zairo, F., additional, Marcello, N., additional, Clorinda, M., additional, Bruna, M., additional, Vincenzo, P., additional, Alessia, P., additional, Giorgio, B., additional, Mair, J., additional, Kremser, C., additional, Aschauer, S., additional, Tufaro, C., additional, Kammerlander, A., additional, Pfaffenberger, S., additional, Marzluf, B., additional, Bonderman, D., additional, Mascherbauer, J., additional, Kliegel, A., additional, Sailer, A., additional, Brustbauer, R., additional, Sedivy, R., additional, Mayr, H., additional, Manessi, M., additional, Castelvecchio, S., additional, Votta, E., additional, Stevanella, M., additional, Menicanti, L., additional, Secchi, F., additional, Redaelli, A., additional, Reiter, U., additional, Reiter, G., additional, Kovacs, G., additional, Greiser, A., additional, Olschewski, H., additional, Fuchsjager, M., additional, Babayev, J., additional, Mlynarski, R., additional, Mlynarska, A., additional, Sosnowski, M., additional, Pontone, G., additional, Bertella, E., additional, Petulla, M., additional, Russo, E., additional, Innocenti, E., additional, Baggiano, A., additional, Mushtaq, S., additional, Gripari, P., additional, Andreini, D., additional, Tondo, C., additional, Nyktari, E., additional, Izgi, C., additional, Haidar, S., additional, Wage, R., additional, Keegan, J., additional, Wong, T., additional, Mohiaddin, R., additional, Durante, A., additional, Rimoldi, O., additional, Laforgia, P., additional, Gianni, U., additional, Benedetti, G., additional, Cava, M., additional, Damascelli, A., additional, Laricchia, A., additional, Ancona, M., additional, Aurelio, A., additional, Pizzetti, G., additional, Esposito, A., additional, Margonato, A., additional, Colombo, A., additional, De Cobelli, F., additional, Camici, P., additional, Zvaigzne, L., additional, Sergejenko, S., additional, Kal js, O., additional, Ripley, D., additional, Swarbrick, D., additional, Hossain, E., additional, Chawner, R., additional, Moore, J., additional, Aquaro, G., additional, Barison, A., additional, Masci, P., additional, Todiere, G., additional, Strata, E., additional, Di Bella, G., additional, Monasterio, F., additional, Levelt, E., additional, Mahmod, M., additional, Ntusi, N., additional, Ariga, R., additional, Upton, R., additional, Piechnick, S., additional, Francis, J., additional, Schneider, J., additional, Stoll, V., additional, Davis, A., additional, Karamitsos, T., additional, Leeson, P., additional, Holloway, C., additional, Clarke, K., additional, Karwat, K., additional, Tomala, M., additional, Miszalski-Jamka, K., additional, Mrozi ska, S., additional, Kowalczyk, M., additional, Mazur, W., additional, Kereiakes, D., additional, Nessler, J., additional, Zmudka, K., additional, Ja wiec, P., additional, Miszalski-Jamka, T., additional, Ben Yaacoub-Kzadri, I., additional, Harguem, S., additional, Bennaceur, R., additional, Ganzoui, I., additional, Ben Miled, A., additional, Mnif, N., additional, Rodriguez Palomares, J., additional, Ortiz, J., additional, Tejedor, P., additional, Lee, D., additional, Wu, E., additional, Bonow, R., additional, Khanji, M., additional, Castiello, T., additional, Westwood, M., additional, Petersen, S., additional, Storti, S., additional, Quota, A., additional, Smacchia, M., additional, Paci, C., additional, Vallone, A., additional, Valeri, G., additional, keilberg, P., additional, Gargani, L., additional, Guiducci, S., additional, Pugliese, N., additional, Pingitore, A., additional, Cole, B., additional, Douglas, H., additional, Rodden, S., additional, Horan, P., additional, Harbinson, M., additional, Johnston, N., additional, Dixon, L., additional, Choudhary, P., additional, Hsu, C., additional, Grieve, S., additional, Semsarian, C., additional, Richmond, D., additional, Celermajer, D., additional, Puranik, R., additional, Hinojar Baydes, R., additional, Varma, N., additional, Goodman, B., additional, Khan, S., additional, Arroyo Ucar, E., additional, Dabir, D., additional, Schaeffter, T., additional, Nagel, E., additional, Puntmann, V., additional, Hinojar, R., additional, Ucar, E., additional, Ngah, N., additional, Kuo, N., additional, D'Cruz, D., additional, Gaddum, N., additional, Foote, L., additional, Schnackenburg, B., additional, Higgins, D., additional, Nucifora, G., additional, Muser, D., additional, Morocutti, G., additional, Gianfagna, P., additional, Zanuttini, D., additional, Piccoli, G., additional, Proclemer, A., additional, Prati, G., additional, Vitrella, G., additional, Allocca, G., additional, Buttignoni, S., additional, Delise, P., additional, Sinagra, G., additional, Silva, G., additional, Almeida, A., additional, David, C., additional, Francisco, A., additional, Magalhaes, A., additional, Placido, R., additional, Menezes, M., additional, Guimaraes, T., additional, Mendes, A., additional, Nunes Diogo, A., additional, Aneq, M., additional, Papavassiliu, T., additional, Sandberg, R., additional, Schimpf, R., additional, Schoenberg, S., additional, Borggrefe, M., additional, Doesch, C., additional, Tamin, S., additional, Tan, L., additional, Joshi, S., additional, Memon, S., additional, Tangcharoen, T., additional, Prasertkulchai, W., additional, Yamwong, S., additional, Sritara, P., additional, Binti Ngah, N., additional, Cruz, D., additional, Rebellato, L., additional, Daleffe, E., additional, Facchin, D., additional, Melao, F., additional, Paiva, M., additional, Pinho, T., additional, Martins, E., additional, Vasconcelos, M., additional, Madureira, A., additional, Macedo, F., additional, Ramos, I., additional, Maciel, M., additional, Agoston-Coldea, L., additional, Marjanovic, Z., additional, Hadj Khelifa, S., additional, Kachenoura, N., additional, Lupu, S., additional, Soulat, G., additional, Farge-Bancel, D., additional, Mousseaux, E., additional, Dastidar, A., additional, Augustine, D., additional, McAlindon, E., additional, Leite, S., additional, Sousa, C., additional, Rangel, I., additional, El ghannudi, S., additional, Lefoulon, A., additional, Noel, E., additional, Germain, P., additional, Doutreleau, S., additional, Jeung, M., additional, Gangi, A., additional, Roy, C., additional, Pisciella, L., additional, Zachara, E., additional, Federica, R., additional, Emdin, M., additional, Baydes, R., additional, Mahmoud, I., additional, and Jackson, T., additional
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- 2014
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24. Myocardial fibrosis by CMR LGE in a large cohort of pediatric thalassemia major patients
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Meloni, A., primary, Filosa, A., additional, Casale, M., additional, Gulino, L., additional, Chiodi, E., additional, Keilberg, P., additional, Armari, S., additional, Positano, V., additional, Lombardi, M., additional, and Pepe, A., additional
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- 2013
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25. 985Myocardial fibrosis by CMR LGE in a large cohort of pediatric thalassemia major patients
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Meloni, A, primary, Filosa, A, additional, Gulino, L, additional, Pulini, S, additional, Salvatori, C, additional, Chiodi, E, additional, Ascioti, C, additional, Keilberg, P, additional, Positano, V, additional, Lombardi, M, additional, and Pepe, A, additional
- Published
- 2013
- Full Text
- View/download PDF
26. 983An automatic method for myocardial T2* curve fitting in thalassemia patients with severe iron overload
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Positano, V, primary, Meloni, A, additional, Santarelli, MF, additional, Landini, L, additional, Tassi, C, additional, Grimaldi, S, additional, Gulino, L, additional, De Marchi, D, additional, Chiodi, E, additional, Renne, S, additional, Lombardi, M, additional, and Pepe, A, additional
- Published
- 2013
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27. Endocrine Effects on Heart Function
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Gamberini, M.R., primary, Meloni, A., additional, Caruso, V., additional, Capra, M., additional, Cianciulli, P., additional, Chiodi, E., additional, Lombardi, M., additional, and Pepe, A., additional
- Published
- 2011
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28. Magnetic resonance imaging for the evaluation of patients undergoing cardiac resynchronization therapy. Advantages, limitations and perspectives | La risonanza magnetica nella valutazione del paziente da sottoporre a resincronizzazione cardiaca. Vantaggi, limiti e prospettive
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Mele, D., Chiodi, E., Lombardi, M., Bighi, S., Feggi, L., Milanesi, M., and Roberto Ferrari
29. Microarray test and hazelnut allergy
- Author
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Mara DE AMICI, Amici, M., Caimmi, S., Ciprandi, G., Caimmi, D., Chiodi, E., Labo, E., Giunta, V., and Marseglia, G.
30. Abstracts
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Doulaptsis, C, Masci, PG, Goetschalckx, K, Janssens, S, Bogaert, J, Ferreira, VM, Piechnik, SK, DallArmellina, E, Karamitsos, TD, Francis, JM, Ntusi, N, Holloway, C, Choudhury, RP, Kardos, A, Robson, MD, Friedrich, MG, Neubauer, S, Miszalski-Jamka, T, Sokolowska, B, Szczeklik, W, Karwat, K, Miszalski-Jamka, K, Belzak, K, Malek, L, Mazur, W, Kereiakes, DJ, Jazwiec, P, Musial, J, Pedrotti, P, Masciocco, G, DAngelo, L, Milazzo, A, Quattrocchi, G, Zanotti, F, Frigerio, M, Roghi, A, Rimoldi, O, Kaasalainen, T, Kivistö, S, Holmström, M, Pakarinen, S, Hänninen, H, Sipilä, O, Lauerma, K, Banypersad, S.M, Fontana, M, Maestrini, V, Sado, D.M, Pinney, J, Wechalekar, A.D, Gillmore, J.D, Lachmann, H, Hawkins, P.N, Moon, J.C, Barone-Rochette, G, Pierard, S, Seldrum, S, de Ravensteen, CM, Melchior, J, Maes, F, Pouleur, A-C, Vancraeynest, D, Pasquet, A, Vanoverschelde, J-L, L Gerber, B, Captur, G, Muthurangu, V, Flett, AS, Wilson, R, Barison, A, Anderson, S, Cook, C, Sado, DM, McKenna, WJ, Mohun, TJ, Elliott, PM, Moon, JC, Pepe, A, Meloni, A, Gulino, L, Rossi, G, Paci, C, Spasisno, A, keilberg, P, Restaino, G, Resta, MC, Positano, V, lombardi, M, Reiter, U, Reiter, G, Kovacs, G, Schmidt, A, Olschewski, H, Fuchsjäger, M, Macmillan, A, Dabir, D, Rogers, T, Monaghan, M, Nagel, E, Puntmann, V, Semaan, E, Spottiswoode, B, Freed, B, Carr, M, Wasielewski, M, Fortney-Campione, K, Shah, S, Carr, J, Markl, M, Collins, J, Sung, YM, Hinojar, R, Ucar, EA, Dabir, D, Voigt, T, Gaddum, N, Schaeffter, T, Nagel, E, Puntmann, VO, Dabir, D, Rogers, T, Ucar, EA, Kidambi, A, Plein, S, Gebker, R, Schnackenburg, B, Voigt, T, Schaeffter, T, Nagel, E, Puntmann, VO, McAlindon, E, Bucciarelli-Ducci, C, Sado, D, Maestrini, V, Piechnik, S, Porter, J, Yamamura, J, Fischer, R, Moon, J, Symons, R, Doulaptsis, C, Masci, P.G, Goetschalckx, K, Dymarkowski, S, Janssens, S, Bogaert, J, Yalin, K, Golcuk, E, Ozer, CS, Buyukbayrak, H, Yilmaz, R, Dursun, M, Bilge, AK, Adalet, K, Reinstadler, SJ, Klug, G, Feistritzer, HJ, Mayr, A, Harrasser, B, Krauter, L, Mair, J, Schocke, MF, Pachinger, O, Metzler, B, Rigolli, M, To, A, Edwards, C, Ding, P, Christiansen, J, Rodríguez-Palomares, JF, Ortiz, JT, Bucciarelli, C, Lee, D, Wu, E, Bonow, RO, Karwat, K, Tomala, M, Miszalski-Jamka, K, Licholaj, S, Mazur, W, Kereiakes, DJ, Nessler, J, Zmudka, K, Jazwiec, P, Miszalski-Jamka, T, Peltonen, J, Kaasalainen, T, Kivistö, S, Holmström, M, Lauerma, K, Rutz, T, Meierhofer, C, Martinoff, S, Ewert, P, Hess, J, Stern, H, Fratz, S, Groarke, JD, Waller, AH, Blankstein, R, Kwong, RY, Steigner, M, Alizadeh, Z, Alizadeh, A, Khajali, Z, Mohammadzadeh, A, Kaykhavani, A, Heidarali, M, Singh, A, Bekele, S, Gunarathne, A, Khan, J, Nazir, SN, Steadman, CD, Kanagala, P, Horsfield, MA, McCann, GP, Duncan, RF, Dundon, BK, Nelson, AJ, Williams, K, Carbone, A, Worthley, MI, Zaman, A, Worthley, SG, Monney, P, Piccini, D, Rutz, T, Vincenti, G, Koestner, S, Stuber, M, Schwitter, J, Gripari, P, Maffessanti, F, Pontone, G, Andreini, D, Bertella, E, Mushtaq, S, Caiani, EG, Pepi, M, El ghannudi, S, Nghiem, A, Germain, P, Jeung, M-J, Roy, C, Gangi, A, Nucifora, G, Muser, D, Masci, PG, Barison, A, Piccoli, G, Rebellato, L, Puppato, M, Gasparini, D, Lombardi, M, Proclemer, A, Nucifora, G, Muser, D, Masci, PG, Barison, A, Piccoli, G, Rebellato, L, Puppato, M, Gasparini, D, Lombardi, M, Proclemer, A, Pöyhönen, P, Kivistö, S, Holmströn, M, Hänninen, H, Thorning, C, Bickelhaupt, S, Kampmann, C, Wentz, KU, Widmer, U, Juli, CF, Miszalski-Jamka, K, Klys, J, Glowacki, J, Kijas, M, Miszalski-Jamka, T, Adamczyk, T, Kwiecinski, R, Bogucka-Czapska, J, Ozaist, M, Mazur, W, Kluczewska, E, Kalarus, Z, Kukulski, T, Karakus, G, Marzluf, B, Bonderman, D, Tufaro, C, Pfaffenberger, S, Babyev, J, Maurer, G, Mascherbauer, J, Kockova, R, Tintera, J, Kautznerova, D, Cerna, D, Sedlacek, K, Kryze, L, El-Husseini, W, Sikula, V, Segetova, M, Kautzner, J, Vasconcelos, M, Lebreiro, A, Martins, E, Cardoso, JS, Madureira, AJ, Ramos, I, Maciel, MJ, Florian, A, Ludwig, A, Rösch, S, Sechtem, U, Yilmaz, A, Monmeneu, J.V, López-Lereu, M.P, Bonanad, C, Sanchis, J, Chaustre, F, Merlos, P, Valero, E, Bodí, V, Chorro, F.J, Yalin, K, Golcuk, E, Ozer, CS, Buyukbayrak, H, Yilmaz, R, Dursun, M, Bilge, AK, Adalet, K, Klug, G, Reinstadler, SJ, Feistritzer, HJ, Mayr, A, Riegler, N, Schocke, M, Esterhammer, R, Kremser, C, Pachinger, O, Metzler, B, Siddiqi, N, Cameron, D, Neil, C, Jagpal, B, Singh, S, Schwarz, K, Papadopoulou, S, Frenneaux, MP, Dawson, D, Robbers, LFHJ, Eerenberg, ES, Teunissen, PFA, Jansen, MF, Hollander, MR, Horrevoets, AJG, Knaapen, P, Nijveldt, R, Levi, MM, van Rossum, AC, Niessen, HWM, Marcu, CB, Beek, AM, van Royen, N, Everaars, H, Robbers, LFHJ, Nijveldt, R, Beek, AM, Teunissen, PFA, Hirsch, A, van Royen, N, Zijlstra, F, Piek, JJ, van Rossum, AC, Goitein, O, Grupper, A, Hamdan, A, Eshet, Y, Beigel, R, Medvedofsky, D, Herscovici, R, Konen, E, Hod, H, Matetzky, S, Cadenas, R, Iniesta, AM, Refoyo, E, Antorrena, I, Guzman, G, Cuesta, E, Salvador, O, López, T, Moreno, M, López-Sendon, JL, Alam, SR, Spath, N, Richards, J, Dweck, M, Shah, A, Lang, N, Semple, S, MacGillivray, T, Mckillop, G, Mirsadraee, S, Pessotto, R, Zamvar, V, Newby, DE, Henriksen, P, Reiter, G, Reiter, U, Kovacs, G, Olschewski, H, Fuchsjäger, M, Ahmad, S, Raza, U, Malik, A, Sun, JP, Eisner, R, Mazur, W, ODonnell, R, Positano, V, Meloni, A, Santarelli, MF, Landini, L, Tassi, C, Grimaldi, S, Gulino, L, De Marchi, D, Chiodi, E, Renne, S, Lombardi, M, Pepe, A, Wu, L, Germans, T, Güçlü, A, Allaart, CP, van Rossum, AC, Kalisz, K, Lehenbauer, K, Katz, D, Bi, X, Cordts, M, Guetter, C, Jolly, M-P, Freed, B, Shah, S, Markl, M, Flukiger, J, Carr, J, Collins, J, Osiak, A, Tyrankiewicz, U, Jablonska, M, Jasinski, K, Jochym, PT, Chlopicki), S, Skorka, T, Kalisz, K, Semaan, E, Katz, D, Bi, X, Cordts, M, Guetter, C, Jolly, MP, Freed, B, Flukiger, J, Lee, D, Kansal, P, Shah, S, Markl, M, Carr, J, Collins, J, Groarke, JD, Shah, RV, Waller, AH, Abbasi, SA, Kwong, RY, Blankstein, R, Steigner, M, Chin, CWL, Semple, S, Malley, T, White, A, Prasad, S, Newby, DE, Dweck, M, Pepe, A, Meloni, A, Lai, ME, Vaquer, S, Gulino, L, De Marchi, D, Cuccia, L, Midiri, M, Vallone, A, Positano, V, Lombardi, M, Pedrotti, P, Milazzo, A, Quattrocchi, G, Roghi, A, Rimoldi, O, Barison, A, De Marchi, D, Masci, P, Milanesi, M, Aquaro, GD, Keilberg, P, Positano, V, Lombardi, M, Positano, Vincenzo, Barison, Andrea, Pugliese, Nicola Riccardo, Masci, Piergiorgio, Del Franco, Annamaria, Aquaro, Giovanni Donato, Landini, Luigi, Lombardi, Massimo, Dieringer, MA, Deimling, M, Fuchs, K, Winter, L, Kraus, O, Knobelsdorff-Brenkenhoff, FV, Schulz-Menger, J, Niendorf, T, Hinojar, R, Ucar, EA, DCruz, D, Sangle, S, Dabir, D, Voigt, T, Gaddum, N, Schaeffter, T, Nagel, E, Puntmann, VO, Sung, YM, Pontone, G, Andreini, D, Bertella, E, Mushtaq, S, Gripari, P, Cortinovis, S, Loguercio, M, Baggiano, A, Conte, E, Pepi, M, El ghannudi, S, Hop, O, Germain, P, Jeung, M-J, De Cesare, A, Roy, C, Gangi, A, Barone-Rochette, G, Pierard, S, Seldrum, S, De Meester de Ravensteen, C, Melchior, J, Maes, F, Pouleur, A-C, Vancraeynest, D, Pasquet, A, Vanoverschelde, J-L, L Gerber, B, Bekele, S, Singh, A, Khan, JN, Nazir, SA, Kanagala, P, McCann, GP, Singh, A, Steadman, CD, Bekele, S, Khan, JN, Nazir, SA, Kanagala, P, McCann, GP, Paelinck, BP, Vandendriessche, T, De Bock, D, De Maeyer, C, Parizel, PM, Christiaan, J, Trauzeddel, RF, Gelsinger, C, Butter, C, Barker, A, Markl, M, Schulz-Menger, J, von Knobelsdorff, F, Florian, A, Schäufele, T, Ludwig, A, Rösch, S, Wenzelburger, I, Yilmaz, A, Sechtem, U, López-Lereu, M.P, Bonanad, C, Monmeneu, J.V, Sanchís, J, Estornell, J, Igual, B, Maceira, A, Chorro, F.J, Focardi, M, Cameli, M, Bennati, E, Massoni, A, Solari, M, Carbone, F, Banchi, B, Mondillo, S, Miia, H, Kirsi, L, Helena, H, Tiina, H, Jyri, L, Pauli, P, Sari, K, Schumm, J, Greulich, S, Grün, S, Ong, P, Klingel, K, Kandolf, R, Sechtem, U, Mahrholdt, H, Raimondi, F, Ou, P, Boudjemline, Y, Bajolle, F, Iserin, F, Bonnet, D, Collins, J, Kalisz, K, Benefield, B, Sarnari, R, Katz, D, Bi, X, Cordts, M, Guetter, C, Jolly, M-P, Freed, B, Flukiger, J, Kansal, P, Lee, D, Shah, S, Markl, M, Carr, J, Sokolowska, B, Miszalski-Jamka, T, Szczeklik, W, Karwat, K, Miszalski-Jamka, K, Belzak, K, Mazur, W, Kereiakes, DJ, Jazwiec, P, Musial, J, Silva, G, Almeida, AG, Resende, C, Marques, JS, Silva, D, David, C, Amaro, C, Costa, P, Silva, JAP, Diogo, AN, Tsokolov, AV, Senchilo, VG, Vertelkin, AV, Hoffmann, P, Mykjåland, G, Wangberg, H, Tønnessen, T, Sjaastad, I, Nordsletten, L, Hjørnholm, U, Løset, A, Rostrup, M, Meloni, A, Gulino, L, Keilberg, P, Palazzi, G, Maddaloni, D, Ascioti, C, Missere, M, Salvatori, C, Positano, V, Lombardi, M, Pepe, A, Meloni, A, Filosa, A, Gulino, L, Pulini, S, Salvatori, C, Chiodi, E, Ascioti, C, Keilberg, P, Positano, V, Lombardi, M, Pepe, A, Meloni, A, Gulino, L, Pietrapertosa, A, Izzi, G, De Marchi, D, Valeri, G, Preziosi, P, Positano, V, Lombardi, M, Pepe, A, Meloni, A, Ruffo, GB, Keilberg, P, Gulino, L, Gerardi, C, Sallustio, G, Tudisca, C, Positano, V, Lombardi, M, Pepe, A, Greulich, S, Backes, M, Schumm, J, Grün, S, Sechtem, U, Mahrholdt, H, Dorniak, K, MSc, AS, Szurowska, E, Fijalkowski, M, Rawicz-Zegrzda, D, Dudziak, M, Raczak, G, Hamdan, A, Baker, FA, Klein, M, Di Segni, E, Goitein, O, Fibisch, G, Konen, E, Müller-Bierl, B, Tanaka, K, Buls, N, Fierens, Y, van Cauteren, T, Willekens, I, van Laere, S, Luypaert, R, de Mey, J, Muzzarelli, S, Faragasso, E, Pedrazzini, G, Sürder, D, Pasotti, E, Moccetti, T, Faletra, F, Qayyum, AA, Hasbak, P, Larsson, HB, Mathiasen, AB, Vejlstrup, NG, Kjaer, A, Kastrup, J, Moschetti, K, Favre, D, Pinget, C, Pilz, G, Petersen, S, Wagner, A, Wasserfallen, JB, Schwitter, J, Ghosh Dastidar, A, Cengarle, M, McAlindon, E, Augustine, D, Nightingale, AK, Bucciarelli-Ducci, C, Dandekar, VK, Ertel, AW, Dickens, C, Gonzalez, RC, Farzaneh-Far, A, Ripley, DP, Higgins, D, McDiarmid, AK, Bainbridge, GJ, Uddin, A, Kidambi, A, Herzog, B, Greenwood, JP, Plein, S, Khanji, M, Newton, T, Westwood, M, Sekhri, N, and Petersen, SE
- Abstract
Background-Aims: Early post-infarction pericardial injury is a common finding but its diagnosis remains elusive. Though C-reactive protein (CRP) is considered a marker of myocardial damage, reflecting myocardial inflammation at the infarcted area, we sought to assess the relationship between CRP and pericardial injury depicted by cardiovascular magnetic resonance (CMR) imaging in patients with ST elevation myocardial infarction (MI). Methods and results: 181 MI patients (84% male) were studied with CMR in the first week and at 4 months post-infarction to assess infarct characteristics, left ventricular volumes/function and pericardial injury. The latter was defined as pericardial fluid >4mm and/or enhancement on late gadolinium enhancement CMR. The CRP-value at day 2 (according to previous literature) was used for correlation with CMR and clinical parameters. Pericardial injury was noted in 87 patients, i.e. effusion (n = 30), inflammation (n = 46), both (n = 11). Patients with pericardial injury had significantly higher peak values of cardiac biomarkers (p<0.001) and higher peak CRP-values than patients with normal pericardium (median 13 vs 43 mg/dl, p<0.001). A strong correlation was found between peak CRP-values and a) left venticular ejection fraction and infarct size both at 1 week and 4 months, b) myocardial hemorrhage, microvascular obstruction (MVO) and pericardial injury at 1 week, c) cardiac biomarkers values and time to PCI. However in a multiple regression model only pericardial injury (p = 0.003) and less importantly time to PCI (p = 0.022) were the independent predictors of CRP values. Conclusion: Pericardial damage described by cardiac MRI occurs often after acute ST elevation MI. CRP-values at the acute phase of MI reflect not only inflammation at the infarcted area but even more the inflammation of the surrounding pericardial tissue.
Table 1 Comparison of baseline clinical and biochemical parameters of patients with or without evidence of early post-infarct pericardial damage on CMR Normal Group (n = 94) Pericardial injury group (n = 87) p-value Agem, years 59±11 60±12 0.48 Male, n(%) 83 (88) 69 (79) 0.10 Diabets, n(%) 12 (13) 9 (10) 0.61 Smoker, n(%) 52 (55) 44 (51) 0.52 Hyperlipidemia, n(%) 56 (60) 55 (63) 0.62 BSA m2 2.0 ± 0.2 2.0 ± 0.2 0.20 Time to PCI, min 195 (155 − 274) 223 (160 − 335) 0.20 Troponin I, μ/l 44 (19 − 92) 90 (44 − 149) >0.001 CK-MB, U/L 128 (77 − 216) 250 (143 − 443) >0.001 CRP, mg/dL 13 (7 − 28) 43 (16 − 96) >0.001 Day of peak CRP 2 (1 − 3) 2 (1 − 3) 0.39 Table 2 Significant correlations between CRP Values and corresponding CMR measurements, cardic biomarkers and clinical related parameters Varibles Spearmanscorrelations r p-value CMR parameters 1 week LV EF −0.28 >0,001 Infractsize(%ofLV) 0.40 >0,001 Microvasular obstruction 0.27 >0,001 Hemorrhage 0.33 >0,001 Size of area atrisk 0.31 >0,001 Transmurality 0.30 >0,001 Pericaldial damage 0.43 >0,001 CMR parameters 4 months LVEF −0.43 >0,001 Infarctsize(%ofLV) 0.46 >0,001 Cardiac Biomarkers Peak TnI 0.34 >0,001 Peak CK-MB 0.32 >0,001 Other Time to PCI 0,182 0,007 - Published
- 2013
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- View/download PDF
31. An innovative approach for the GDPR compliance in Big Data era
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Massimiliano Giacalone, Carlo Cusatelli, Fabio Fanari, Vito Santarcangelo, Diego Carmine Sinitò, Antonino Abbruzzo Eugenio Brentari Marcello Chiodi e Davide Piacentino, Giacalone, Massimiliano, Cusatelli, Carlo, Fanari, Fabio, Santarcangelo, Vito, Carmine Sinitò, Diego, and Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino
- Subjects
Big Data ,GDPR ,privacy ,GDPR, privacy, Big Data - Abstract
The present work shows a preliminary overview of the Big Data Analytics scenario, introducing the related privacy issues considered by the new General Data Protection Regulation, better known by its acronym GDPR. The work then introduces an innovative index to assess the compliance of a company with this regulation on the protection of personal data, in terms of privacy by design and privacy by default. Abstract Il presente lavoro mostra una preventiva panoramica in merito allo scenario del Big Data Analytics, introducendo le relative problematiche di privacy considerate dal nuovo Regolamento Generale sulla Protezione dei Dati, meglio noto con l’acronimo inglese GDPR. Il lavoro introduce quindi un innovativo indice per valutare la conformità di un’azienda a tale regolamento sulla tutela dei dati personali, in termini di privacy by design e privacy by default.
- Published
- 2018
32. Network-based dimensionality reduction for textual datasets
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Michelangelo Misuraca, Germana Scepi, Maria Spano, Michelangelo Misuraca, Germana Scepi, Maria Spano, E. Brentari M. Chiodi E.-J. C. Wit., Misuraca, Michelangelo, Scepi, Germana, and Spano, Maria
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- 2023
33. PC Algorithm for Gaussian Copula Data
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Vitale, Vincenzina, Paola, Vicard, A. Abbruzzo, E. Brentari, M. Chiodi e D. Piacentino, Vitale, Vincenzina, and Vicard, Paola
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Bayesian networks ,Structural learning, Bayesian networks, R-vines, Gaussian copulae, PC algorithm ,PC algorithm ,Gaussian copulae ,Structural learning ,R-vines ,Statistics::Computation - Abstract
The PC algorithm is the most popular algorithm used to infer the structure of a Bayesian network directly from data. For Gaussian distributions, it infers the network structure using conditional independence tests based on Pearson correlation coefficients. Here, we propose two modified versions of PC, the R-vine PC and D-vine PC algorithms, suitable for elliptical copula data. The correlation matrix is inferred by means of the estimated structure and parameters of a regular vine. Simulation results are provided, showing the very good performance of the proposed algorithms with respect to their main competitors.
- Published
- 2018
34. The Pricing Behaviour of Firms in the On-line Accommodation Market: Evidence from a Metropolitan City
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Andrea Guizzardi, Flavio Maria Emanuele Pons, Ercolino Ranieri, Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, and Andrea Guizzardi, Flavio Maria Emanuele Pons, Ercolino Ranieri
- Subjects
RevPOR, RevPAR, Dynamic Pricing, panel-VAR - Abstract
The widespread diffusion of on-line travel agencies has opened the possibility, for hoteliers, to update continously quality and prices offered along the advance booking. We study firms’ pricing behaviour in a business-oriented environment considering time series of daily best available rates for 107 hotels in Milan, over a period of 9 months, from 0 to 28 days of advance booking. Throught a panel-VAR approach we assess if the typical planning of the price trajectory, including dummies for holidays and fairs as covariates. Results suggest that strategies put into effect by firms reflect some of the basic principles of the online revenue management. Price trajectories are planned considering both firms expectations on the prices they hope to charge last-minute, and their need to guarantee price stability along the advance booking. Fairs and holidays show a different impact on price dynamics. While the response caused by an ”holiday shock” tends to be flat, room rates during fairs raise in the immediate future, then accommodate to the equilibrium in about three days.
- Published
- 2018
35. How can we compare rankings that are expected to be similar? An example based on composite well being indicators
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Silvia Terzi, Luca Moroni, Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, Terzi, Silvia, and Moroni, Luca
- Published
- 2018
36. Statistical matching by Bayesian Networks
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Marella, Daniela, Vicard, Paola, Vitale, Vincenzina, A. Abbruzzo, E. Brentari, M. Chiodi e D. Piacentino, Marella, Daniela, Vicard, Paola, and Vitale, Vincenzina
- Subjects
Statistical Matching, Bayesian Networks, uncertainty ,Statistical Matching ,Bayesian Networks ,uncertainty - Abstract
The goal of statistical matching is the estimation of the joint distribution of variables not jointly observed in a sample survey but separately available from independent sample surveys. The lack of joint information on the variables of interest leads to uncertainty about the data generating model. In this paper we propose the use of Bayesian networks to deal with the statistical matching problem since they admit a recursive factorization of a joint distribution useful for evaluating the statistical matching uncertainty in the multivariate context.
- Published
- 2018
37. Heterogeneous effects of subsidies on farms’ performance: a spatial quantile regression analysis
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de castris marusca, DI GENNARO, DANIELE, DE CASTRIS MARUSCA, DI GENNARO DANIELE, Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, DE CASTRIS, Marusca, and DI GENNARO, Daniele
- Subjects
Spatial Quantile Regression, Agricultural Policies, Policy efficacy - Abstract
Italian agricultural sector is characterized by a wide heterogeneity which can affect the effectiveness of rural policies and, by consequence, economic performances. Indeed, wide differences arise both at farm (i.e. sector, dimension, etc.) and regional levels. In particular, Giannakis and Bruggeman (2015) show how agricultural policies can provide enlarge regional disparities between advanced and lagged regions. In this paper, we analyse the differential impact of the policies by considering Italian lagged regions. The introduction of a Spatial Autoregressive Quantile model allows to take into account both spatial and farm-specific characteristic. Evidences are found in favour of significant and positive spatial spillovers of the policies, especially for the less performing farms.
- Published
- 2018
38. A comparative study of benchmarking procedures for interrater and intrarater agreement studies
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Amalia Vanacore, Maria Sole Pellegrino, Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi e Davide Piacentino, Vanacore, Amalia, and Pellegrino, MARIA SOLE
- Published
- 2018
39. Pacing transmural scar tissue reduces left ventricle reverse remodeling after cardiac resynchronization therapy
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Alice Calabrese, Giuseppe Marchese, Eustachio Agricola, Antonello D'Andrea, Elisabetta Chiodi, Fausto Rigo, Patrizia Della Valentina, Rodolfo Citro, Roberto Ferrari, Alessandro Dal Monte, Donato Mele, Maurizio Galderisi, Mele, Donato, Agricola, Eustachio, Monte, Alessandro Dal, Galderisi, Maurizio, D'Andrea, Antonello, Rigo, Fausto, Citro, Rodolfo, Chiodi, Elisabetta, Marchese, Giuseppe, Valentina, Patrizia Della, Calabrese, Alice, Ferrari, Roberto, Mele, D, Agricola, E, Monte, Ad, Galderisi, M, D'Andrea, A, Rigo, F, Citro, R, Chiodi, E, Marchese, G, Valentina, Pd, Calabrese, A, and Ferrari, R.
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Scar tissue ,Cardiac resynchronization therapy ,Heart failure ,Cicatrix ,Magnetic resonance imaging ,Text mining ,Retrospective Studie ,Internal medicine ,Humans ,Medicine ,Prospective Studies ,Reverse remodeling ,Retrospective Studies ,Ultrasonography ,Aged ,Cardiomyopathie ,Ventricular Remodeling ,medicine.diagnostic_test ,business.industry ,Cardiac Pacing, Artificial ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,Prospective Studie ,Treatment Outcome ,medicine.anatomical_structure ,Echocardiography ,Ventricle ,Cardiology ,Female ,Radiology ,Cardiomyopathies ,Cardiology and Cardiovascular Medicine ,business ,Catheter placement ,Human - Abstract
Background In patients with ischemic heart failure undergoing cardiac resynchronization therapy (CRT) the underlying myocardial substrate at the left ventricle (LV) pacing site may affect CRT response. However, the effect of delivering the pacing stimulus remote, adjacent to or over LV transmural scar tissue (TST) identified by echocardiography is still unknown. Methods First, 35 patients with healed myocardial infarction (57±11 years) were prospectically studied to demonstrate the capability of echocardiographic end-diastolic wall thickness (EDWT) to identify LV-TST as defined by delayed enhancement magnetic resonance imaging (DE-MRI). Subsequently, in 136 patients (65±10 years) who underwent CRT, EDWT was retrospectively evaluated at baseline. The LV catheter placement was defined over, adjacent to and remote from TST if pacing was delivered at a scarred segment, at a site 1 segment adjacent to or remote from scarred segments. CRT response was defined as LV end-systolic volume (ESV) decrease by at least 10% after 6months. Results A EDWT≤5mm identified TST at DE-MRI with 92% sensitivity and 96% specificity. In the 76 CRT responders, less overall and posterolateral TST segments and more segments paced remote from TST areas were found. At the multivariate regression analysis, the number of TST segments and scar/pacing relationship showed a significant association with CRT response. Conclusions In addition to LV global scar burden, CRT response relates also to the myocardial substrate underlying pacing site as evaluated by standard echocardiography. This information may expand the role of echocardiography to guide pacing site avoiding pacing at TST areas.
- Published
- 2013
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40. Characterization of Receptor Binding Affinity for Vascular Endothelial Growth Factor with Interferometric Imaging Sensor.
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Lortlar Ünlü N, Bakhshpour-Yucel M, Chiodi E, Diken-Gür S, Emre S, and Ünlü MS
- Subjects
- Humans, Bevacizumab, Receptors, Vascular Endothelial Growth Factor, Biosensing Techniques, Protein Binding, Recombinant Fusion Proteins, Macular Degeneration metabolism, Vascular Endothelial Growth Factor A metabolism, Interferometry
- Abstract
Wet Age-related macular degeneration (AMD) is the leading cause of vision loss in industrialized nations, often resulting in blindness. Biologics, therapeutic agents derived from biological sources, have been effective in AMD, albeit at a high cost. Due to the high cost of AMD treatment, it is critical to determine the binding affinity of biologics to ensure their efficacy and make quantitative comparisons between different drugs. This study evaluates the in vitro VEGF binding affinity of two drugs used for treating wet AMD, monoclonal antibody-based bevacizumab and fusion protein-based aflibercept, performing quantitative binding measurements on an Interferometric Reflectance Imaging Sensor (IRIS) system. Both biologics can inhibit Vascular Endothelial Growth Factor (VEGF). For comparison, the therapeutic molecules were immobilized on to the same support in a microarray format, and their real-time binding interactions with recombinant human VEGF (rhVEGF) were measured using an IRIS. The results indicated that aflibercept exhibited a higher binding affinity to VEGF than bevacizumab, consistent with previous studies using ELISA and SPR. The IRIS system's innovative and cost-effective features, such as silicon-based semiconductor chips for enhanced signal detection and multiplexed analysis capability, offer new prospects in sensor technologies. These attributes make IRISs a promising tool for future applications in the development of therapeutic agents, specifically biologics.
- Published
- 2024
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41. Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.
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Bakhshpour M, Chiodi E, Celebi I, Saylan Y, Ünlü NL, Ünlü MS, and Denizli A
- Subjects
- Interferometry, Biosensing Techniques, Immunoglobulin G
- Abstract
Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 μg/mL with a low detection limit (0.25 μg/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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42. The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors.
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Chiodi E, Marn AM, Bakhshpour M, Lortlar Ünlü N, and Ünlü MS
- Abstract
The field of biosensing is in constant evolution, propelled by the need for sensitive, reliable platforms that provide consistent results, especially in the drug development industry, where small molecule characterization is of uttermost relevance. Kinetic characterization of small biochemicals is particularly challenging, and has required sensor developers to find solutions to compensate for the lack of sensitivity of their instruments. In this regard, surface chemistry plays a crucial role. The ligands need to be efficiently immobilized on the sensor surface, and probe distribution, maintenance of their native structure and efficient diffusion of the analyte to the surface need to be optimized. In order to enhance the signal generated by low molecular weight targets, surface plasmon resonance sensors utilize a high density of probes on the surface by employing a thick dextran matrix, resulting in a three-dimensional, multilayer distribution of molecules. Despite increasing the binding signal, this method can generate artifacts, due to the diffusion dependence of surface binding, affecting the accuracy of measured affinity constants. On the other hand, when working with planar surface chemistries, an incredibly high sensitivity is required for low molecular weight analytes, and furthermore the standard method for immobilizing single layers of molecules based on self-assembled monolayers (SAM) of epoxysilane has been demonstrated to promote protein denaturation, thus being far from ideal. Here, we will give a concise overview of the impact of tridimensional immobilization of ligands on label-free biosensors, mostly focusing on the effect of diffusion on binding affinity constants measurements. We will comment on how multilayering of probes is certainly useful in terms of increasing the sensitivity of the sensor, but can cause steric hindrance, mass transport and other diffusion effects. On the other hand, probe monolayers on epoxysilane chemistries do not undergo diffusion effect but rather other artifacts can occur due to probe distortion. Finally, a combination of tridimensional polymeric chemistry and probe monolayer is presented and reviewed, showing advantages and disadvantages over the other two approaches.
- Published
- 2022
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43. Multiplexed, High-Sensitivity Measurements of Antibody Affinity Using Interferometric Reflectance Imaging Sensor.
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Marn AM, Needham J, Chiodi E, and Ünlü MS
- Subjects
- Antibody Affinity immunology, Humans, Interferometry, Ligands, Surface Plasmon Resonance methods, Anthrax
- Abstract
Anthrax lethal factor (LF) is one of the enzymatic components of the anthrax toxin responsible for the pathogenic responses of the anthrax disease. The ability to screen multiplexed ligands against LF and subsequently estimate the effective kinetic rates (kon and koff) and complementary binding behavior provides critical information useful in diagnostic and therapeutic development for anthrax. Tools such as biolayer interferometry (BLI) and surface plasmon resonance imaging (SPRi) have been developed for this purpose; however, these tools suffer from limitations such as signal jumps when the solution in the chamber is switched or low sensitivity. Here, we present multiplexed antibody affinity measurements obtained by the interferometric reflectance imaging sensor (IRIS), a highly sensitive, label-free optical biosensor, whose stability, simplicity, and imaging modality overcomes many of the limitations of other multiplexed methods. We compare the multiplexed binding results obtained with the IRIS system using two ligands targeting the anthrax lethal factor (LF) against previously published results obtained with more traditional surface plasmon resonance (SPR), which showed consistent results, as well as kinetic information previously unattainable with SPR. Additional exemplary data demonstrating multiplexed binding and the corresponding complementary binding to sequentially injected ligands provides an additional layer of information immediately useful to the researcher.
- Published
- 2021
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44. Multiplexed Affinity Measurements of Extracellular Vesicles Binding Kinetics.
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Chiodi E, Daaboul GG, Marn AM, and Ünlü MS
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- Antibodies, Interferometry, Kinetics, Staining and Labeling, Extracellular Vesicles
- Abstract
Extracellular vesicles (EVs) have attracted significant attention as impactful diagnostic biomarkers, since their properties are closely related to specific clinical conditions. However, designing experiments that involve EVs phenotyping is usually highly challenging and time-consuming, due to laborious optimization steps that require very long or even overnight incubation durations. In this work, we demonstrate label-free, real-time detection, and phenotyping of extracellular vesicles binding to a multiplexed surface. With the ability for label-free kinetic binding measurements using the Interferometric Reflectance Imaging Sensor (IRIS) in a microfluidic chamber, we successfully optimize the capture reaction by tuning various assay conditions (incubation time, flow conditions, surface probe density, and specificity). A single (less than 1 h) experiment allows for characterization of binding affinities of the EVs to multiplexed probes. We demonstrate kinetic characterization of 18 different probe conditions, namely three different antibodies, each spotted at six different concentrations, simultaneously. The affinity characterization is then analyzed through a model that considers the complexity of multivalent binding of large structures to a carpet of probes and therefore introduces a combination of fast and slow association and dissociation parameters. Additionally, our results confirm higher affinity of EVs to aCD81 with respect to aCD9 and aCD63. Single-vesicle imaging measurements corroborate our findings, as well as confirming the EVs nature of the captured particles through fluorescence staining of the EVs membrane and cargo.
- Published
- 2021
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45. EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles.
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Brambilla D, Sola L, Ferretti AM, Chiodi E, Zarovni N, Fortunato D, Criscuoli M, Dolo V, Giusti I, Murdica V, Kluszczyńska K, Czernek L, Düchler M, Vago R, and Chiari M
- Subjects
- Drug Delivery Systems, Magnetic Phenomena, Exosomes, Extracellular Vesicles, Nanoparticles
- Abstract
Extracellular vesicles (EVs) have attracted considerable interest due to their role in cell-cell communication, disease diagnosis, and drug delivery. Despite their potential in the medical field, there is no consensus on the best method for separating micro- and nanovesicles from cell culture supernatant and complex biological fluids. Obtaining a good recovery yield and preserving physical characteristics is critical for the diagnostic and therapeutic use of EVs. The separation of a single class of EVs, such as exosomes, is complex because blood and cell culture media contain many nanoparticles in the same size range. Methods that exploit immunoaffinity capture provide high-purity samples and overcome the issues of currently used separation methods. However, the release of captured nanovesicles usually requires harsh conditions that hinder their use in certain types of downstream analysis. A novel capture and release approach for small extracellular vesicles (sEVs) is presented based on DNA-directed immobilization of antiCD63 antibody. The flexible DNA linker increases the capture efficiency and allows for releasing EVs by exploiting the endonuclease activity of DNAse I. This separation protocol works under mild conditions, enabling the release of vesicles suitable for analysis by imaging techniques. In this study, sEVs recovered from plasma were characterized by established techniques for EV analysis, including nanoparticle tracking and transmission electron microscopy.
- Published
- 2021
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46. The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview.
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Chiodi E, Marn AM, Geib MT, and Ünlü MS
- Abstract
The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules' functionalities is critically analyzed.
- Published
- 2021
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47. Bulk-Effect-Free Method for Binding Kinetic Measurements Enabling Small-Molecule Affinity Characterization.
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Marn AM, Chiodi E, and Ünlü MS
- Abstract
Optical technologies for label-free detection are an attractive solution for monitoring molecular binding kinetics; however, these techniques measure the changes in the refractive index, making it difficult to distinguish surface binding from a change in the refractive index of the analyte solution in the proximity of the sensor surface. The solution refractive index changes, due to solvents, temperature changes, or pH variations, can create an unwanted background signal known as the bulk effect. Technologies such as biolayer interferometry and surface plasmon resonance offer no bulk-effect compensation, or they alternatively offer a reference channel to correct in postprocessing. Here, we present a virtually bulk-effect-free method, without a reference channel or any computational correction, for measuring kinetic binding using the interferometric reflectance imaging sensor (IRIS), an optical label-free biomolecular interaction analysis tool. Dynamic spectral illumination engineering, through tailored LED contributions, is combined with the IRIS technology to minimize the bulk effect, with the potential to enable kinetic measurements of a broader range of analytes. We demonstrate that the deviation in the reflectivity signal is reduced to ∼8 × 10
-6 for a solution change from phosphate-buffered saline (PBS) ( n = 1.335) to 1% dimethyl sulfoxide (DMSO) in PBS ( n = 1.336). As a proof of concept, we applied the method to a biotin-streptavidin interaction, where biotin (MW = 244.3 Da) was dissolved at a final concentration of 1 μM in a 1% solution of DMSO in PBS and flowed over immobilized streptavidin. Clear binding results were obtained without a reference channel or any computational correction., Competing Interests: The authors declare the following competing financial interest(s): M. Selim Unlu is the founder the startup iRiS Kinetics, Inc., which is working towards the commercialization of the IRIS technique., (© 2021 The Authors. Published by American Chemical Society.)- Published
- 2021
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48. A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications.
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Chiodi E, Damin F, Sola L, Ferraro L, Brambilla D, Ünlü MS, and Chiari M
- Abstract
The manufacture of a very high-quality microarray support is essential for the adoption of this assay format in clinical routine. In fact, poorly surface-bound probes can affect the diagnostic sensitivity or, in worst cases, lead to false negative results. Here we report on a reliable and easy quality control method for the evaluation of spotted probe properties in a microarray test, based on the Interferometric Reflectance Imaging Sensor (IRIS) system, a high-resolution label free technique able to evaluate the variation of the mass bound to a surface. In particular, we demonstrated that the IRIS analysis of microarray chips immediately after probe immobilization can detect the absence of probes, which recognizably causes a lack of signal when performing a test, with clinical relevance, using fluorescence detection. Moreover, the use of the IRIS technique allowed also to determine the optimal concentration of the probe, that has to be immobilized on the surface, to maximize the target recognition, thus the signal, but to avoid crowding effects. Finally, through this preliminary quality inspection it is possible to highlight differences in the immobilization chemistries. In particular, we have compared NHS ester versus click chemistry reactions using two different surface coatings, demonstrating that, in the diagnostic case used as an example (colorectal cancer) a higher probe density does not reflect a higher binding signal, probably because of a crowding effect.
- Published
- 2021
- Full Text
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49. [Role of cardiac imaging in the diagnosis of infective endocarditis].
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Mele D, Flamigni F, Cultrera R, Cittanti C, Chiodi E, Fiorencis A, and Ferrari R
- Subjects
- Diagnosis, Differential, Fever of Unknown Origin, Humans, Leukocytes, Practice Guidelines as Topic, Symptom Assessment, Echocardiography methods, Endocarditis diagnostic imaging, Multidetector Computed Tomography methods, Positron-Emission Tomography methods, Tomography, Emission-Computed, Single-Photon methods
- Abstract
Infective endocarditis is an increasingly common disease in the hospital setting. Although the 2015 guidelines of the European Society of Cardiology deal extensively with many aspects of infective endocarditis, there are still unsolved problems related to diagnosis, in particular to the appropriate use of cardiac imaging methods, that require further study. The aim of this review is to analyze the advantages and limitations of the echocardiographic, radiological and nuclear imaging methods in order to identify diagnostic pathways applicable in clinical practice.
- Published
- 2020
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50. Instrument-Free Protein Microarray Fabrication for Accurate Affinity Measurements.
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Celebi I, Geib MT, Chiodi E, Lortlar Ünlü N, Ekiz Kanik F, and Ünlü S
- Subjects
- Interferometry, Oligonucleotide Array Sequence Analysis, Proteins, Biosensing Techniques, Protein Array Analysis
- Abstract
Protein microarrays have gained popularity as an attractive tool for various fields, including drug and biomarker development, and diagnostics. Thus, multiplexed binding affinity measurements in microarray format has become crucial. The preparation of microarray-based protein assays relies on precise dispensing of probe solutions to achieve efficient immobilization onto an active surface. The prohibitively high cost of equipment and the need for trained personnel to operate high complexity robotic spotters for microarray fabrication are significant detriments for researchers, especially for small laboratories with limited resources. Here, we present a low-cost, instrument-free dispensing technique by which users who are familiar with micropipetting can manually create multiplexed protein assays that show improved capture efficiency and noise level in comparison to that of the robotically spotted assays. In this study, we compare the efficiency of manually and robotically dispensed α-lactalbumin probe spots by analyzing the binding kinetics obtained from the interaction with anti-α-lactalbumin antibodies, using the interferometric reflectance imaging sensor platform. We show that the protein arrays prepared by micropipette manual spotting meet and exceed the performance of those prepared by state-of-the-art robotic spotters. These instrument-free protein assays have a higher binding signal (~4-fold improvement) and a ~3-fold better signal-to-noise ratio (SNR) in binding curves, when compared to the data acquired by averaging 75 robotic spots corresponding to the same effective sensor surface area. We demonstrate the potential of determining antigen-antibody binding coefficients in a 24-multiplexed chip format with less than 5% measurement error.
- Published
- 2020
- Full Text
- View/download PDF
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