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31 results on '"Cho, Mi Ae"'

8. Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation

Author

Liu Jang-Ryol, Song Yun-Mi, Cho Mi-Ae, Min Sung-Ran, Ko Suck-Min, Choi Pil-Son, Lee Jun-Haeng, and Park Yun-Ok

Subjects
Reporter gene, biology, Agrobacterium, Melon, fungi, food and beverages, biology.organism_classification, Molecular biology, Transformation (genetics), chemistry.chemical_compound, Glufosinate, chemistry, Botany, Cucumis, Transformation efficiency, Explant culture
Abstract

Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf (), flower (), seeds () and plantlet (). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

Published
2005

9. The Use of Glufosinate as a Selective Marker for the Transformation of Cucumber (Cucumis sativus L.)

Author

Liu Jang-Ryol, Cho Mi-Ae, Song Yun-Mi, Min Sung-Ran, Choi Pil-Son, Ko Suck-Min, and Park Yun-Ok

Subjects
biology, Agrobacterium, fungi, food and beverages, biology.organism_classification, Molecular biology, chemistry.chemical_compound, Transformation (genetics), Glufosinate, chemistry, Botany, Gene, Cucumis, Selectable marker, Explant culture, Southern blot
Abstract

Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.

Published
2005

10. Yellowish Friable Embryogenic Callus (YFEC) Production and Plant Regeneration from Immature Embryo Cultures of Domestic Maize Cultivars and Genotypes (Zea may L.)

Author

Park Yun-Ok, Liu Jang-Ryol, Kim Jin-Suck, Park Ki-Jin, Cho Mi-Ae, Choi Pil-Son, and Min Hwang-Ki

Subjects
Horticulture, chemistry.chemical_compound, Sucrose, Somatic embryogenesis, chemistry, Callus, Botany, Casamino acid, Embryo, Proline, Cultivar, Biology, Explant culture
Abstract

Immature embryos of 3 cultivars (Du Me Chal, Mi Baek Chal, Heug Jeom Chal) and 5 genotypes (HW1, KL103, HW3, HW4, KW7) were cultured on medium containing MS salts, Eriksson`s vitamins, 1 mg/L 2,4-dichlorophenoxyacetic acid, 25 mM proline, 100 mg/L casamino acid, 3 mM MES, 1.7 mg/L and 20 g/L sucrose (SIM). Frequency of somatic embryo formation on explant of immature embryos showed in HW1 (45.20%), KL103 (5.75%), HW3 (37.20%), HW4 (30.10%), KW70 (55.20%), Mi Baek Chal (18.74%), Heug Jeom Chal (22.41%), Du Me Chal (36.72%) and Hi II type (

Published
2005

22. A Case of Graves' Disease Presenting with Chorea

Author

Ku, Cheol Ryong, primary, Park, Hyung Jun, additional, Hong, Sung Jin, additional, Shin, Dong Yeob, additional, Lee, Jin Ha, additional, Chung, Moon Jae, additional, Cho, Mi Ae, additional, Noh, Tae Woong, additional, Lee, Byung In, additional, and Lee, Eun Jig, additional

Published
2008
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25. Expression and role of estrogen receptor α and β in medullary thyroid carcinoma: different roles in cancer growth and apoptosis

Author

Cho, Mi Ae, primary, Lee, Mi Kyung, additional, Nam, Kee-Hyun, additional, Chung, Woung Youn, additional, Park, Cheong Soo, additional, Lee, Ju Hyeong, additional, Noh, Taewoong, additional, Yang, Woo Ick, additional, Rhee, Yumie, additional, Lim, Sung-Kil, additional, Lee, Hyun Chul, additional, and Lee, Eun Jig, additional

Published
2007
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30. Echogenic carotid artery plaques are associated with vertebral fractures in postmenopausal women with low bone mass.

Author

Se Hwa Kim, Yoo Mee Kim, Mi Ae Cho, Yumie Rhee, Kyu Yeon Hur, Eun Seok Kang, Bong Soo Cha, Eun Jig Lee, Hyun Chul Lee, Sung Kil Lim, Kim, Se Hwa, Kim, Yoo Mee, Cho, Mi Ae, Rhee, Yumie, Hur, Kyu Yeon, Kang, Eun Seok, Cha, Bong Soo, Lee, Eun Jig, Lee, Hyun Chul, and Lim, Sung Kil

Subjects
CAROTID artery, ARTERIES, CAROTID sinus, BONE diseases, ACHONDROPLASIA, BLOOD vessels, ATHEROSCLEROTIC plaque
Abstract

Although low bone mass has been associated with atherosclerosis even after adjustment for age, little is known about the association between vertebral fractures and calcified atherosclerotic plaques. Our objective was to investigate whether osteoporotic vertebral fractures are independently related to the prevalence of atherosclerotic carotid plaques in postmenopausal women with low bone mass. We enrolled 195 postmenopausal women with osteopenia or osteoporosis. Bone mineral density and the presence of vertebral fractures were assessed. Intima media thickness and atherosclerotic plaques of the carotid artery were assessed using ultrasonography. Of the 195 subjects in the study, 84 had no plaques and 111 had at least one. The percentage of women with vertebral fractures was significantly higher in subjects with echogenic carotid plaques than in those without (27% vs. 11%, respectively; P < 0.05). However, there was no difference in the prevalence of vertebral fractures between women with echolucent plaques and those without (10.9% vs. 10.7%, respectively; P = nonsignificant). By logistic regression analysis with multivariate adjustment, age (P < 0.01), dyslipidemia (P < 0.05), and the presence of vertebral fracture (P < 0.05) were independent risk factors for echogenic carotid plaques. Osteoporotic vertebral fractures are associated with an increased risk of echogenic atherosclerotic plaques in postmenopausal women with low bone mass. It appears that the high association of echogenic atherosclerotic plaques and vertebral fractures could partially explain why osteoporotic vertebral fractures are linked to increased mortality. [ABSTRACT FROM AUTHOR]

Published
2008
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31. Expression and role of estrogen receptor alpha and beta in medullary thyroid carcinoma: different roles in cancer growth and apoptosis.

Author

Cho MA, Lee MK, Nam KH, Chung WY, Park CS, Lee JH, Noh T, Yang WI, Rhee Y, Lim SK, Lee HC, and Lee EJ

Subjects
Adenoviridae genetics, Carcinoma, Medullary metabolism, Estradiol analogs & derivatives, Estradiol pharmacology, Estrogen Antagonists pharmacology, Estrogen Receptor alpha genetics, Estrogen Receptor beta genetics, Estrogens metabolism, Estrogens pharmacology, Fulvestrant, Gene Transfer Techniques, Genes, Reporter drug effects, Genetic Vectors, Humans, Immunohistochemistry, Ki-67 Antigen metabolism, Response Elements genetics, Thyroid Neoplasms metabolism, Transcription Factor AP-1 metabolism, Transcription, Genetic, Apoptosis, Carcinoma, Medullary physiopathology, Cell Division, Estrogen Receptor alpha metabolism, Estrogen Receptor beta metabolism, Thyroid Neoplasms physiopathology
Abstract

Medullary thyroid carcinoma (MTC) originates from parafollicular C cells. Estrogen receptor beta(ERbeta) expressionwas detected in normal parafollicular C cells and MTC tumor tissue, but ERalpha expression in MTC tumors still remains undetermined. The appearance and loss of ERalpha or ERbeta expression has been known to play a role in the development and progression of many human cancers. We performed immunohistochemical studies of ERalpha, ERbeta, and Ki67, a mitotic index, in 11 human MTC tissue samples. ERalpha was detected in 10 cases (91%), and ERbeta expression was observed in 8 cases (72.7%). A majority (8/10) of ERalpha-positive tumors showing ERbeta Ki67 expression was detected in three cases (27.3%). Neither clinical parameters nor tumor node metastasis (TNM) tumor staging was correlated with the positivity for ERs or Ki67. To investigate the biological role of each ER, we used ER-negative MTC TT cells and adenoviral vectors carrying ERalpha (Ad-ERalpha), ERbeta (Ad-ERbeta), estrogen response element (ERE)-Luc (Ad-ERE-Luc), and activator protein 1 (AP1)-Luc (Ad-AP1-Luc). Estrogen stimulated and anti-estrogen, ICI 182 780, suppressed ERE reporter activity in TT cells expressing ERalpha or ERbeta, suggesting that both ERs use the same classical ERE-mediated pathway. Ad-ERalpha infection stimulated TT cell growth; in contrast, Ad-ERbeta infection suppressed their growth. Apoptosis was detected in Ad-ERbeta-infected TT cells. Estrogen and anti-estrogen suppressed AP1 activity in Ad-ERalpha-infected cells, whereas upon Ad-ERbeta infection estrogen further stimulated AP1 activity which in turn is suppressed by anti-estrogen, suggesting that each ER acts differently through a non-ERE-mediated pathway. Our results suggest that ERalpha and ERbeta may play different roles in MTC tumor growth and progression.

Published
2007
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