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Mitochondria-associated endoplasmic reticulum membranes (MAMs) regulate ATG14- and Beclin1-mediated mitophagy and play key roles in the development of diabetic nephropathy (DN). DsbA-L is mainly located in MAMs and plays a role in renoprotection, but whether it activates mitophagy by maintaining MAM integrity remains unclear. In this study, we found that renal tubular damage was further aggravated in diabetic DsbA-L-/- mice compared to diabetic mice and that this damage was accompanied by disrupted MAM integrity and decreased mitophagy. Furthermore, notably decreased expression of ATG14 and Beclin1 in MAMs extracted from the kidneys of diabetic DsbA-L-/- mice was observed. In vitro, overexpression of DsbA-L reversed the disruption of MAM integrity and enhanced mitophagy in HK-2 cells, a human proximal tubular cell line, after exposure to high-glucose (HG) conditions. Additionally, compared to control mice, DsbA-L-/- mice were exhibited downregulated expression of helicase with zinc finger 2 (HELZ2) in their kidneys according to transcriptome analysis; HELZ2 serves as a cotranscription factor that synergistically functions with PPARα to promote the expression of mitofusin 2 (MFN-2). Treatment of HK-2 cells with MFN-2 siRNA resulted in MAM uncoupling and decreased mitophagy. Moreover, HG notably reduced the expression of HELZ2 and MFN-2 and inhibited mitophagy, and these effects were partially blocked by overexpression of DsbA-L and altered upon cotreatment with HELZ2 siRNA, HELZ2 overexpression or MK886 (PPARα inhibitor) treatment. These data indicate that DsbA-L alleviates diabetic tubular damage by activating mitophagy through maintenance of MAM integrity via the HELZ2/MFN-2 pathway.

This article investigates the dependence of transmitting sensitivity on the top electrode design of piezoelectric micromachined ultrasonic transducers (PMUTs). Two typical top electrodes, namely inner electrode (IE) and outer electrode (OE), are designed and fabricated. The measured transmitting velocities of the fabricated PMUTs at resonance under a drive voltage of 5 $\text{V}_{\mathrm {p-p}}$ (peak-to-peak) are 15.36 mm/s for the IE design and 20.67 mm/s for the OE design with a circular diaphragm and 16.62 mm/s for the IE design and 22.18 mm/s for the OE design with a hexagonal diaphragm. The OE design demonstrates a transmitting velocity improvement of 34.57% for the circular diaphragm and 33.45% for the hexagonal diaphragm. The improvement is due to the fact that the OE design shows higher quality factor ( ${Q}$ ) than the IE counterpart. Moreover, the resonant frequency of the OE design is higher than that of the IE design, which results in a larger acoustic pressure output and hence higher transmitting sensitivity. This work highlights an effective and simple approach for PMUTs to achieve high transmitting sensitivity, which is an important parameter in the applications that require large sound pressures, such as fingerprint imaging, gesture recognition, and ranging.

In this article, we present a high-performance aluminum nitride (AlN)-based microelectromechanical system (MEMS) hydrophone sensor, in which the sensing cells are designed and arranged as an innovative honeycomb architecture for achieving large acoustic pressure sensitivity and high fill-factor. An $8\times9$ array of $360~{\mu }\text{m}$ in characteristic size, 0.92-MHz MEMS hydrophone sensor is developed based on an AlN-on-cavity silicon-on-insulator (CSOI) platform. The size of the MEMS hydrophone sensor is 3.2 mm $\times3.2$ mm. The MEMS hydrophone sensor and its preamplification circuit are integrated on a printed circuit board and packaged together with an acoustically transparent material, in order to meet the stringent requirements of underwater applications. The packaged MEMS hydrophone is fully characterized through using an industry-standard hydrophone calibration instrument. The MEMS hydrophone achieves an acoustic pressure sensitivity of −178 dB (re: 1 V/ $\mu$ Pa), with a maximum nonlinearity of 0.1%, and a noise resolution of 58.7 dB (re: 1 $\mu$ Pa/Hz). The measurement results show that the reported MEMS hydrophone sensor with bioinspired honeycomb structure is compared favorable with advanced commercially available bulky hydrophones.

The transforming growth factor-β (TGF-β)/Smads signal plays an important role in cancer metastasis by mediating the epithelial-mesenchymal transition (EMT) in cancer cells. lnc-TSI is a recently identified long noncoding RNA that negatively regulates the TGF-β/Smads signal. The present study was conducted to test the hypothesis that lnc-TSI inhibits metastasis in clear cell renal cell carcinoma (ccRCC) by regulating the TGF-β/Smad3 pathway. Herein, we show that lnc-TSI was upregulated in ccRCC cells and tissue and was associated with activation of the TGF-β/Smads signal. Depleting lnc-TSI enhanced tumor cell invasion and metastasis in vitro and ccRCC lung metastasis in vivo, whereas overexpressing lnc-TSI inhibited ccRCC cell invasion and tumor metastasis. Mechanistic studies indicated that lnc-TSI specifically inhibited the phosphorylation of Smad3 and subsequent EMT by binding with the MH2 domain of Smad3 to block the interaction between Smad3 and TGF-β receptor I in ccRCC cells. In a cohort of 150 patients with ccRCC, expression of lnc-TSI in tumors was negatively correlated with phosphorylated (p)Smad3 and activated EMT markers. Patients with expression of tumor lnc-TSI greater than or equal to the median at radical nephrectomy had a higher survival rate compared to those with lnc-TSI below the median during follow-up. These findings reveal a new regulatory mechanism of ccRCC metastasis and suggest a potential molecular target for the development of anti-cancer drugs., Graphical Abstract, TGF-β/Smad3 signaling has been found to play an important role in cancer metastasis. Wang et al. show that lncRNA lnc-TSI inhibits cancer cell invasion and tumor metastasis via specifically inhibiting the phosphorylation of Smad3 and subsequent EMT, which could be potentially applied in treatments for cancer metastasis.

Cisplatin, as a commonly used anticancer drug, can easily lead to acute kidney injury (AKI), and has received more and more attention in clinical practice. β-hydroxybutyric acid (BHB) is a metabolite in the body and acts as an inhibitor of oxidative stress and NLRP3 inflammasome, reducing inflammatory responses and apoptosis. However, the role of BHB in cisplatin-induced AKI is currently not fully elucidated. In this study, C57BL/6 male mice were randomly divided into normal control group, cisplatin-induced AKI group and AKI with BHB treatment group. Compared to the control, cisplatin-treated mice exhibited high level of serum creatinine, blood urea nitrogen and severe tubular injury, which accompanied with significantly increased expression level of NLRP3, IL-1β, IL-18, BAX, cleaved-caspase 3, as well as aggravated oxidative stress and renal tubular cell apoptosis. However, these changes were significantly improved in that of BHB treatment. In vitro, our study showed that the expression of cleaved-caspase3, IL-1β and IL-18 were significantly increased in human proximal tubular epithelial cell line (HK-2) treated with cisplatin compared with the control group, while decreased in cells treated with BHB. Furthermore, a significantly increased expression of cGAS and STING in HK-2 cells treated with cisplatin were found, whereas notably decreased in cells treated with BHB. This data indicates that BHB protects against cisplatin-induced AKI and renal tubular damage mediated by NLRP3 inflammasome and cGAS-STING pathway.

This work sought to investigate whether lycopene administration modulated the expression of two major Aβ transporters, i.e., the low-density lipoprotein receptor protein-1 (LRP1) and the receptor for advanced glycation end-products (RAGE) being localized to the choroid plexus (CP) in the Alzheimer’s disease (AD) rats. Forty-eight adult male Wistar rats were assigned into four equal groups. Results showed that lycopene administration attenuated Aβ accumulation, increased the LRP1 levels, as well as decreased the RAGE levels in the cerebrospinal fluid (CSF) or the CP. Furthermore, lycopene administration significantly enhanced antioxidant enzymatic system for inactivating and controlling ROS, suppressed RAGE/nuclear factor-κB (NF-κB) signaling pathway and the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) in the CP of AD rats. Our data presented in this report provided the firsthand evidence that lycopene administration might provide a novel therapeutic approach to improve Aβ clearance and prevent CP epithelial cells neuroinflammation in AD.

OBJECTIVE: To investigate the effect of palbociclib on cell cycle progression and proliferation of human renal tubular epithelial cells. METHODS: Human renal tubular epithelial cell line HK-2 was treated with 1, 5, 10, and 20 μmol/L of palbociclib, and the changes in cell proliferation and viability were examined by cell counting and CCK8 assay. EDU staining was used to assess the proliferation of HK-2 cells following palbiciclib treatment at different concentrations for 5 days. The effect of palbociclib on cell cycle distribution of HK-2 cells was evaluated using flow cytometry. SA-β-Gal staining and C12FDG senescence staining were used to detect senescence phenotypes of HK-2 cells after palbociclib treatment at different concentrations for 5 days. The relative mRNA expression levels of P16, P21, and P53 and the genes associated with senescence-related secretion phenotypes were detected by RT-PCR, and the protein expressions of P16, P21 and P53 were detected by Western blotting. RESULTS: Palbociclib inhibited HK-2 cell proliferation and induced cell cycle arrest in G1 phase. Compared with the control cells, HK-2 cells treated with high-dose (10 μmol/L) palbociclib exhibited significantly suppressed cell proliferation activity, and the inhibitory effect was the most obvious on day 5 (P < 0.01). Palbociclib treatment significantly reduced the number of cells in S phase (P < 0.01) and induced senescence of HK-2 cells. The results of SA-β-Gal and C12FDG senescence staining showed a significantly enhanced activity of intracellular senescence-related galactosidase in palbociclib-treated HK-2 cells, suggesting significant senescence of the cells (P < 0.01). RT-PCR and Western blotting showed that palbociclib treatment significantly increased the mRNA and protein expression levels of P16, P21, and P53 in HK-2 cells (P < 0.01); the mRNA expression levels of senescence-related secretory factors also increased significantly in HK-2 cells after palbociclib treatment (P < 0.01). CONCLUSIONS: Palbociclib induces HK-2 cell senescence by causing cell growth arrest and delaying cell cycle progression.

Acute kidney injury (AKI) is a common clinical problem, and patients who survive AKI have a high risk of chronic kidney disease (CKD). The mechanism of CKD post-AKI, characterized by progressive renal fibrosis, is still unclear. Maladaptive tubular epithelial cells (TECs) after AKI are considered a leading cause of renal fibrosis post-AKI. TECs under maladaptive repair manifest characteristics of senescence. Removing senescent TECs by genetic ablation has been proven effective in reducing renal fibrosis. Senolytics, which eliminate senescent cells by pharmacological intervention, have been studied in a series of degenerative diseases. To our knowledge, the effects of senolytics on renal fibrosis post-AKI have not been verified before. Here, we confirmed renal senescence in the unilateral ischemia/reperfusion injury murine model. Senescent TECs could activate fibroblasts and senolytics specifically induced apoptosis of senescent TECs. Next, we demonstrated that senolytics could reduce renal senescence and ameliorate renal fibrosis in both unilateral renal ischemia/reperfusion injury and multiple-cisplatin-treatment murine models. Our results indicate senescent TECs as a vital factor in renal fibrosis progression, and senolytic therapy might be promising for treating CKD post-AKI.

In this paper, a low noise amplification circuit is designed for aluminum nitride-based piezoelectric micromachined ultrasonic transducers (PMUTs) with three different sensing configurations. The acoustic pressure sensitivity and noise resolution of PMUTs corresponding to different readout configurations are analyzed and characterized. With the differential readout configuration, the acoustic pressure sensitivity of the PMUTs is improved by 5.76 dB (re: 1 V µPa−1) on average, and the noise resolution is reduced by about 8 dB at 500 Hz (re: 1 µPa (Hz 1 / 2 )−1). Compared to the conventional readout configuration, both theoretical analysis and measurement results show that the output voltage of PMUTs is doubled with the differential readout configuration and thus the acoustic pressure sensitivity is improved. Moreover, the noise resolution of PMUTs is reduced and thus the signal-to-noise is improved. This work offers new insight on improving the receiving performance of PMUTs.

OBJECTIVE: To investigate the effect of blocking pannexin-1 against acute kidney injury induced by cisplatin. METHODS: Twenty-six male C57BL/6 mice aged 6-8 weeks were randomly divided into control group, cisplatin model (Cis) group and cisplatin + carbenoxolone treatment group (Cis + CBX). In Cis group and Cis + CBX group, the mice were injected intraperitoneally with 20 mg/kg of cisplatin and with CBX (20 mg/kg) at 30 min before and 24 and 48 h after cisplatin inhjection, respectively. All the mice were sacrificed at 72 h after cisplatin injection, and plasma and kidney samples were collected for testing mRNA and protein expression levels of pannexin-1 in the renal tissue using RT-qPCR and Western blotting and for detecting plasma creatinine and BUN levels; the pathological changes in the renal tissues were observed using Periodic Acid-Schiff staining. The expression of kidney injury molecule 1 (KIM-1) was examined using immunohistochemistry and the mRNA expressions of KIM-1 and neutrophil gelatinase- related lipid transport protein (NGAL) were detected by RT-qPCR to evaluate the injuries of the renal tubules. The infiltration of F4/80-positive macrophages and CD4-positive T cells were observed by immunofluorescence. In the in vitro experiment, human proximal tubule epithelial cell line HK-2 was stimulated with 50 μmol/L cisplatin to establish a cell model of acute kidney injury, and the mRNA and protein expressions of pannexin-1 were detected by RT-qPCR and Western blotting at 4, 6, 12, 18 and 24 h after the stimulation. RESULTS: Compared with the control mice, the cisplatin-treated mice showed significantly up-regulated protein levels (P < 0.05) and mRNA levels (P < 0.005) of pannexin-1 in the kidney tissue. Cisplatin stimulation also caused significant increases in the protein levels (P < 0.005) and mRNA levels (P < 0.005) of pannexin-1 in cultured HK-2 cells. Compared with cisplatin-treated mice, the mice treated with both cisplatin and the pannexin-1 inhibitor CBX showed obviously lessened kidney pathologies and milder renal tubular injuries with significantly reduced plasma BUN and Scr levels (P < 0.01), expressions of KIM-1 and NGAL in the kidney (P < 0.05), and infiltration of F4/80-positive macrophages (P < 0.01) and CD4- positive T cells (P < 0.05) in the kidney tissues. CONCLUSION: In cisplatin induced acute kidney injury mice model, Pannexin-1 expression is up-regulated in the kidneys tissue, and blocking pannexin-1 alleviates the acute kidney injury via reducing renal inflammatory cell infiltration.

The availability of zebrafish larvae model of epileptic seizure provides opportunities to identify novel anticonvulsants for treatment of people with epilepsy. However, the major parameters of Zebrafishbehavior assay for assessing anti-epileptic drug efficacy existed disparity. In this study, we chose the high, medium, slow-speed moved distances and the total distances moved for seizure-like activity quantification in individual wells of a 48-well plate in the dark phase. Results also showed that the high-speed moved distances were given a more reasonable and sensitive dose-response curve than the total distances in zebrafish larvae model exposed to 10 mMpentylenetetrazole (PTZ). Moreover, this seizure-like swimming pattern was alleviated by the addition of either phenytoin (PHT) or valproate sodium (VPA), two commonly prescribed anti-epileptic drugs (AEDs). They exhibited concentration-dependent inhibition of both locomotor activity and PTZ-induced c-fostranscription, confirming their anticonvulsant characteristics. Whereas carbamazepine (CBZ) promoted zebrafish larvae movement by 26% at 30 μM and 116.7% at 100 μM, and sharply suppressed zebrafish larvae motility about 94.8% at 300 μM.C-fos expression significantly decreased at 30 μM and 300 μM CBZ, which was found to oppose various aspects of the PTZ-induced changes in activity. These results indicated that this zebrafish larvae model could be useful for studying drug efficacy, predicting drug targets and aiding our understanding of disease etiology.

Neuroinflammation characterized by glial activation and release of proinflammatory mediators is considered to be correlated with cognitive deficits in Alzheimer's disease (AD). Previously, some studies have demonstrated that lycopene (LYCO) or human amniotic epithelial cells (HAECs) could attenuate inflammation in AD. Specifically, the choroid plexus (CP), an epithelial layer that forms the blood-cerebrospinal fluid barrier, is able to modulate the cognitive function, through changes in the neuroinflammatory response and in brain immune surveillance. However, it is unclear if LYCO can interact with HAECs to improve neuroinflammation at the CP. Thus, this study chose the region of interest, considered the feasibility of using a combination of LYCO and HAECs, as a therapeutic agent for immunomodulatory effects at the CP in an acutely induced AD rat model. Results showed that oral administration of LYCO, HAECs transplantation, and their combination significantly improved cognitive deficits in water maze test, decreased the level of proinflammatory mediators (TNF-α and IL-1β), increased the level of anti-inflammatory mediators (IL-10 and TGF-β1) in the cerebro-spinal fluid, and hippocampal tissue. Interestingly, LYCO administration, HAECs transplantation and their combination reversed the Aβ1–42 induced up-regulation of Toll like receptor 4 and nuclear factor-κB p65 mRNA and protein expressions at the CP. This study provided the novel experimental evidence for the influence of co-treatment with LYCO and HAECs on immunomodulatory capabilities of CP. It could also warrant therapeutic window for the pathophysiology of AD and the associated underlying mechanisms at the CP.

The mechanism underlying the development of chronic kidney disease (CKD) after acute kidney injury (AKI) remains unclear. Maladaptive repair has been considered an important mechanism of CKD post AKI. Renal tubular cells under maladaptive repair have characteristics of premature senescence. These premature senescent cells can generate profibrotic factors that promote organ fibrosis. The purpose of this study was to investigate whether cisplatin induces premature renal senescence and the role of premature renal senescence in the progression of CKD post AKI. As oxidative stress is a major cause of senescence, we further evaluated whether antioxidant therapy could protect renal tubular cells from cisplatin-induced premature senescence and retard the progression of CKD post AKI. The molecular mechanism of this protection was also investigated. We found that cisplatin induced premature renal senescence in vitro and in vivo. In a multiple-cisplatin-treatment murine model, renal interstitial fibrosis was accompanied by premature renal senescence. N-acetylcysteine (NAC), an antioxidant, attenuated premature senescence and decreased renal fibrosis, and its effects were dependent on sirtuin1 (SIRT1) activation and p53 deacetylation. These results indicate that cisplatin can induce premature renal senescence, which is associated with the development of CKD post cisplatin-induced AKI. SIRT1 activation and p53 deacetylation might be identified as potential targets for attenuating premature renal senescence and retarding the progression of CKD post AKI.

Epidemiological studies show that maternal cigarette smoking is associated with an increased risk of cardiovascular diseases in postnatal life. Baroreflex sensitivity (BRS) is an important index for evaluating the homeostasis of the cardiovascular system. This experiment was designed to investigate the possible mechanism of prenatal nicotine on the adult male offspring’s heart rate (HR) increase due to BRS. Pregnant rats received the 0.3 ml of saline or nicotine (1.5 mg kg−1) by subcutaneous injection from gestational days 3 to 21. The male offsprings of saline injected dams were the control group, and the male offsprings of the nicotine injected dams were the nicotine group. The 90-day-old male offsprings’ funny current (I f) of their sinoatrial node (SAN) cells, BRS induced by intravenous injection of angiotensin (Ang) II in the presence or absence of the L-nitro-arginine methylester (L-NAME), cervical vagal activity, c-fos protein levels of the cervical spinal cord-8 to the thoracic spinal cord-5 (C8–T5) lateral horn neuron, and blood hormones were tested. The results showed that prenatal nicotine exposure had no effect on the offsprings’ I f of their SAN cells, but it significantly decreased the offsprings’ BRS. The c-fos protein levels of the C8–T5 lateral horn neurons and the blood catecholamine levels were increased in the nicotine group, but the cervical vagal activity was not changed. After intraventricular injection of L-NAME, the nicotine exposed offsprings’ BRS was partly recovered. These data suggest that prenatal nicotine exposure results in hyper reactivity of the spinal sympathetic nerve center and a higher peripheral catecholamine hormone state of 90-day-old male offsprings, and these may be the reason for the BRS inhibition and HR increase. Nitric oxide (NO) may participate in the process acting as an important neurotransmitter.

The mucilage polysaccharide was extracted from Dioscorea opposita in cold water and then degraded in two reagents hydrogen peroxide and ascorbic acid. Three low-molecular-weight-samples were prepared, and their antioxidant and antimutagenic activity were investigated. Chemical composition analysis indicated that the degradation action was in a concentration dependent manner. Total sugars content of three degraded samples were significantly higher than raw sample. The uronic acid content in the degraded sample LP3 was significantly higher than other samples. LP3 processed the higher scavenging effect on hydroxyl and superoxide radicals than other two degraded samples because of its lower molecular weight and more uronic acid. LP3 processed the excellent antimutagenic activity and higher anti-lipid peroxidation in garlic roots. There maybe a certain relationship between the two activities. The present results indicated this mucilage could be a potential candidate of the natural antimutagen.

In this study, the antimutagenic function of the polysaccharide from Enteromorpha linza with the micronucleus test of Allium sativum root cells induced by sulfur dioxide and ultraviolet was studied. The concentration-effect relation of the two inducers was firstly evaluated. The results showed that an increase of genotoxicity damage was demonstrated and micronuclei frequency induced by sulfur dioxide and ultraviolet displayed dose dependent increases. All the doses of polysaccharide did affect the micronuclei frequency formation compared with the negative control. And also, the significant increase in inhibition rate of micronuclei frequency was observed with the increase of the dose of polysaccharide. It was showed maximum inhibition of micronuclei frequency cells (71.74% and 66.70%) at a concentration of 200 g/mL in three experiments. The low molecular weight polysaccharide showed higher inhibition rate than raw polysaccharide at the higher concentration (50 g/mL) in the absence of sulfur dioxide and ultraviolet. It was confirmed to be a good mutant inhibitor. (C) 2016 Elsevier B.V. All rights reserved.

The availability of zebra-fish larvae model of epileptic seizure provides opportunities to identify novel anticonvulsants for treatment of people with epilepsy. However, the major parameters of zebra-fish behavior assay for assessing antiepileptic drug efficacy existed disparity, which resulted in different results in previous studies. In this study, we chose the high, medium, slow-speed moved distances and the total distances moved for seizure-like activity quantification in individual wells of a 48-well plate in the dark phase and used this zebra-fish seizure model to assess three commonly prescribed anti-epileptic drug’s efficacy and screen bioactive components from plant extract. Results showed that the high-speed moved distances were given a more reasonable and sensitive dose-response curve than the total distances in zebra-fish larvae model exposed to 10 mM pentylene tetrazole. Besides, we also optimized the vehicle (dimethyl sulfoxide, DMSO) concentration used in epileptic behaviour assay. The three anti-epileptic drugs, phenytoin, valproate sodium and carbamazepine showed the same efficacy patterns in zebra-fish seizure model as in mammalian epileptic models. We also found four positive hits from plant (Vaccinium spp.) extract in primary drug screening, two hits exhibited concentration-dependent inhibition of locomotor activity, confirming their anticonvulsant characteristics. These results indicated that this zebra-fish larvae model could be useful for assessing anti-epileptic drug efficacy, facilitating the primary drug screening and evaluating of effective components in medicinal plants.

Typically, small lizards rely heavily on behavioral thermoregulation rather than physiological mechanisms to control their rates of warming and cooling. We tested the hypothesis that prostaglandins participate in mediating the cardiovascular response to heating and cooling and temperature regulating neurons in the hypothalamus of the small lizard Phrynocephalus przewalskii. In vivo and in vitro treatments, heart rates (HRs) were all found to be higher during heating than during cooling, hysteresis was distinct below 30 and 26 degrees Celsius, respectively. In vivo, as administration of COX inhibitor, there were no differences in HR between heating and cooling at any body temperature and administration of agonist prostaglandins only produced a significant effect on HR below 25 degrees Celsius. Single-unit activity was recorded extracellularly in vitro with microelectrodes, found the firing rate of the continuous unit increased 23% when the temperature of the artificial cerebrospinal fluid dropped from 30-20 degrees Celsius. We conclude that prostaglandins appear to play only a limited role in modulating heart activity in Phrynocephalus przewalskii and suggest that cold-sensitive neurons in the preoptic and anterior hypothalamus (PO/AH) are involved in thermoregulatory control during heating or cooling.

Se realizo el presente estudio histologico de las celulas sanguineas de Bufo Bufo gargarizans en diferentes meses del ano: enero, marzo, mayo, julio y octubre. Fueron utilizados metodos de rutina por frotis de sangre con tincion de Wright y observacion in vivo. Encontramos dos tipos principales de celulas de globulos rojos al frotis como tambien en celulas in vivo: mitoticas y amitoticas. Por cuanto amitosis se produce durante todo el ano, sobre todo en el mes de julio, la mitosis hasta el momento se habia observado solamente en julio. Ademas, se encontro una gran cantidad de neutrofilos en los globulos de Bufo Bufo gargarizans. Los nucleos de estas celulas son polimorficos, especialmente en enero y marzo. La concentracion de globulos rojos era mas bajo en mayo y mas alta en enero; la concentracion de las celulas blancas de la sangre fue mayor en octubre y menor en marzo. En cuanto a los granulocitos, eosinofilos estos presentaron una mayor proporcion en julio y octubre, mientras que los neutrofilos y basofilos registraran una mayor proporcion en el mes de julio. Los agranulocitos y las celulas mononucleares alcanzaron el valor mas alto en marzo, y el valor mas bajo en enero. Los linfocitos y el valor maximo fue registrado en mayo, el valor mas bajo fue registrado en julio. No fueron evidentes los cambios morfologicos de trombocitos, lo que podria tener relacion con su estabilidad.

To investigate the changes of expression on leptin, a series of neuroendocrine factors and hormones associated with body weight control and energy balance regulation of rats, which were treated with repeated fasting/refeeding and followed by fed with high fat diet.Designing a repeated fasting/refeeding rats model (RFR) fed with basic stock diet on repeated cycles of 1 d fasting and 1 d refeeding for 6 weeks. The rats in RFR-LF/ HF group were switched to a high fat diet and fed the diet every day for another 6 weeks. The control rats were randomly divided into 3 groups, control group, high-fat diet (HF) group and common fat diet (CF) group. The rats in HF and CF group were killed by the end of the 12th week. The body weight, Lee's index, body fat content and serum lipid, GH, T4, leptin, insulin, and plasma ACTH levels were measured. The expression of NPY and POMC mRNA in hypothalamus were detected by reverse transcription chain reaction (RT-PCR).The Lee's index, body fat content, serum TC, TG, LDL, leptin and insulin levels of RFR-LF/HF group were lower significantly than those of HF group whereas higher significantly than those of CF group. The expression of NPY mRNA of RFR-LF/HF group were higher significantly than those of HF and CF groups, while the expression of POMC mRNA was lower significantly than that of HF and CF groups.The feeding pattern of repeated fasting/refeeding can decrease the degree of obesity induced by high fat diet, and also reduce the leptin and insulin resistance, but cause serious disturbance of the expression of neuroendocrine peptides in the central nervous system of rat.