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The amount of maternal antibody transferred to the egg and the amount taken up by the developing chicks are important parameters that may greatly influence the health and survival of the chicks. Thus, the study of these parameters might provide a new likelihood in improving the livability of chicks. Considering the short life span of broiler chicks from hatch to slaughter, the importance of maternal antibodies is highly relevant. Therefore, the study was conducted to compare genetic variations in transfer of maternal immunoglobulin Y (IgY) through egg yolk in different broiler sire and dam lines namely: Male Line White (MLW), Male Line Colour (MLC), Male Line White 2 (MLW2), Female Line White (FLW), Female Line Colour (FLC) and a two-way cross of MLW male and FLW female (MLW x FLW). In total, 42 freshly laid eggs were collected from apparently healthy hens; 7 each from six different lines of chicken. Egg yolk IgY of the collected eggs was isolated by polyethylene glycol (PEG) precipitation method with slight modifications. Furthermore, the purity of the isolated IgY was examined by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The egg yolk IgY levels were significantly (p<0.001) varied among the studied lines of chicken. The total amount of IgY per milliliter of yolk was the highest in FLW (30.904 ± 7.621) and the lowest in MLW (16.753 ± 5.282). However, the calculated total amount of yolk IgY (mg/egg) contained by the entire egg yolk was the highest (392.030 ± 136.185) in MLW2 and was the lowest (206.015 ± 61.058) in FLC and it was varied between 206.015 and 392.030 in the experimental lines of chicken. This study concluded that genotype of chickens has significant effect on transferability of maternal IgY through egg yolk in different meat lines of chickens.