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3. A procedure for removal of cyanuric acid in swimming pools using a cell-free thermostable cyanuric acid hydrolase

Author

Guo, Feng, primary, McAuliffe, Joseph C, additional, Bongiorni, Cristina, additional, Latone, Jacob A, additional, Pepsin, Mike J, additional, Chow, Marina S, additional, Dhaliwal, Raj S, additional, Hoffmann, Katherine M, additional, Brazil, Bill T, additional, Heng, Meng H, additional, Robinson, Serina L, additional, Wackett, Lawrence P, additional, and Whited, Gregory M, additional

Published
2021
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5. Sulfur K-edge XAS and DFT calculations on nitrile hydratase: Geometric and electronic structure of the non-heme iron active site

Author

Dey, Abhishek, Chow, Marina, Taniguchi, Kayoko, Lugo-Mas, Priscilla, Davin, Steven, Maeda, Mizuo, Kovacs, Julie A., Odaka, Masafumi, Hodgson, Keith O., Hedman, Britt, and Solomon, Edward I.

Subjects
Chemistry
Abstract

Sulfur K-edge X-ray absorption spectroscopy (XAS) and density functional theory (DFT) calculations are used to find the geometric and electronic structure of the active site of the non-heme iron enzyme nitrile hydratase (NHase). The pre-edge region of the X-ray absorption spectrum is sensitive to the Zeff of the Fe and discloses that the Fe in [FeNO](super 6) NHase species has a Zeff very similar to that of its photolyzed Fe(super III) counterpart.

Published
2006

6. Spectroscopic studies of the interaction of ferrous bleomycin with DNA

Author

Kemsley, Jyllian N., Wasinger, Erik C., Zaleski, Kelly Loeb, Hedman, Britt, Chow, Marina S., Hodgson, Keith O., Decker, Andrea, Soloman, Edward I., and Shishova, Ekaterina Y.

Subjects
Circular dichroism -- Usage, DNA -- Chemical properties, DNA -- Spectra, Bleomycin -- Chemical properties, Bleomycin -- Spectra, Chemistry
Abstract

Magnetic circular dichroism (MCD) and X-ray absorption (XAS) spectroscopies are used to study the interaction of the biologically relevant Fe(super II)BLM complex with DNA. Parallel studies of BLM structural derivatives indicate that Fe(super II)iso-PEPLM, in which the carbonyl group is shifted on the mannose sugar, forms the same DNA-bound species as Fe(super II)BLM.

Published
2003

7. A procedure for removal of cyanuric acid in swimming pools using a cell-free thermostable cyanuric acid hydrolase.

Author

Guo, Feng, McAuliffe, Joseph C, Bongiorni, Cristina, Latone, Jacob A, Pepsin, Mike J, Chow, Marina S, Dhaliwal, Raj S, Hoffmann, Katherine M, Brazil, Bill T, Heng, Meng H, Robinson, Serina L, Wackett, Lawrence P, and Whited, Gregory M

Subjects
CYANURIC acid, SWIMMING pools, CELL culture, HOT tubs, CARBON dioxide, THERMAL stability
Abstract

Cyanuric acid (CYA) is used commercially for maintaining active chlorine to inactivate microbial and viral pathogens in swimming pools and hot tubs. Repeated CYA addition can cause a lack of available chlorine and adequate disinfection. Acceptable CYA levels can potentially be restored via cyanuric acid hydrolases (CAH), enzymes that hydrolyze CYA to biuret under mild conditions. Here we describe a previously unknown CAH enzyme from Pseudolabrys sp. Root1462 (CAH-PR), mined from public databases by bioinformatic analysis of potential CAH genes, which we show to be suitable in a cell-free form for industrial applications based upon favorable enzymatic and physical properties, combined with high-yield expression in aerobic cell culture. The kinetic parameters and modeled structure were similar to known CAH enzymes, but the new enzyme displayed a surprising thermal and storage stability. The new CAH enzyme was applied, following addition of inexpensive sodium sulfite, to hydrolyze CYA to biuret. At the desired endpoint, hypochlorite addition inactivated remaining enzyme and oxidized biuret to primarily dinitrogen and carbon dioxide gases. The mechanism of biuret oxidation with hypochlorite under conditions relevant to recreational pools is described. [ABSTRACT FROM AUTHOR]

Published
2022
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8. Excerpts from Sojourner IV - Pilipinos, anthology, 1974-06

Author

Bahng, Hyo-Jin, 1955-; Chan, Donna, 1955-; Chang, Grace, 1957-; Choi, Christine, 1957-; Chow, Marina, 1956-; Cocallas, Isa, 1957-; Endo, Brian, 1957-; Engalla, Dennis, 1958-; Engalla, Emlyn, 1956-; Flores, Soledad, 1957-; Fong, Lyman, 1956-; Fong, Russell, 1954-; Fuertes, Dennis, 1958-; Gock, Lorraine, 1956-; Hayashida, Irene, 1956-; Hing, Cathy, 1956-; Hirasawa, Bobby, 1957-; Huey, Barry, 1957-; Huey, Y. Lee, 1957-; Huey, Mickey, 1958-; Huey, Milly, 1956-; Huey, Tommy, 1959-; Hum, Debbie, 1957-; Igarashi, Patti, 1957-; Itow, Mark, 1956-; Kita, Kim, 1953-; Kubota, Sandy, 1958-; Lai, Shirley, 1956-; Lam, Andrew, 1957-; Lam, Stephen; Lau, Cindy, 1956-; Lee, Anna, 1957-; Lum, Kin, 1958-; Lum, Shuk, 1957-; Mah, Ava, 1957-; Mah, Grant, 1958-; Mah, Ronald, 1953-; Matsumoto, Fumi; Matsumoto, Jocelyn, 1957-; Mayeda, Paul, 1958-; Mishima, Tommy, 1956-; Morizono, Lesli-Jo, 1957-; Nagahisa, Donna, 1957-; Nakagaki, Nancy, 1957-; Nakamura, Cubby, 1957-; Nakaso, Andy, 1956-; Nakaya, Joi, 1957-; Narimatsu, Kimi, 1956-; Noblejas, Connie, 1954-; Oda, Eddie, 1956-; Okano, Bruce, 1956-; On, Sharon, 1956-; Ouye, Joey. 1957-; Ouye, Tami, 1957-; Parvahan, Kooroosh, 1955-; Pham, Thanh; Seid, Mei, 1957-; Seid, Wing; Shimoko, Janet, 1956-; Suda, Vicki, 1957-; Suwanvaichkij, Thavorn, 1955-; Suwanvaichkij, Uthaivan, 1957-; Suwanvaichkij, Vilailok, 1956-; Takahashi, Rickey, 1957-; Tanaka, Doris, 1956-; Tom, Perry, 1958-; Tom, Steven, 1957-; Toyofuku, Lesley, 1956-; Wing, Linda; Wong, Danny Y., 1953-; Wong, Julie, 1956-; Woo, Tommy, 1956-; Woo, Wesley, 1957-; Yee, David, 1956-; Yee, Dorise, 1957-; Yee, Edward, 1957, Asian Writers Project, Bahng, Hyo-Jin, 1955-; Chan, Donna, 1955-; Chang, Grace, 1957-; Choi, Christine, 1957-; Chow, Marina, 1956-; Cocallas, Isa, 1957-; Endo, Brian, 1957-; Engalla, Dennis, 1958-; Engalla, Emlyn, 1956-; Flores, Soledad, 1957-; Fong, Lyman, 1956-; Fong, Russell, 1954-; Fuertes, Dennis, 1958-; Gock, Lorraine, 1956-; Hayashida, Irene, 1956-; Hing, Cathy, 1956-; Hirasawa, Bobby, 1957-; Huey, Barry, 1957-; Huey, Y. Lee, 1957-; Huey, Mickey, 1958-; Huey, Milly, 1956-; Huey, Tommy, 1959-; Hum, Debbie, 1957-; Igarashi, Patti, 1957-; Itow, Mark, 1956-; Kita, Kim, 1953-; Kubota, Sandy, 1958-; Lai, Shirley, 1956-; Lam, Andrew, 1957-; Lam, Stephen; Lau, Cindy, 1956-; Lee, Anna, 1957-; Lum, Kin, 1958-; Lum, Shuk, 1957-; Mah, Ava, 1957-; Mah, Grant, 1958-; Mah, Ronald, 1953-; Matsumoto, Fumi; Matsumoto, Jocelyn, 1957-; Mayeda, Paul, 1958-; Mishima, Tommy, 1956-; Morizono, Lesli-Jo, 1957-; Nagahisa, Donna, 1957-; Nakagaki, Nancy, 1957-; Nakamura, Cubby, 1957-; Nakaso, Andy, 1956-; Nakaya, Joi, 1957-; Narimatsu, Kimi, 1956-; Noblejas, Connie, 1954-; Oda, Eddie, 1956-; Okano, Bruce, 1956-; On, Sharon, 1956-; Ouye, Joey. 1957-; Ouye, Tami, 1957-; Parvahan, Kooroosh, 1955-; Pham, Thanh; Seid, Mei, 1957-; Seid, Wing; Shimoko, Janet, 1956-; Suda, Vicki, 1957-; Suwanvaichkij, Thavorn, 1955-; Suwanvaichkij, Uthaivan, 1957-; Suwanvaichkij, Vilailok, 1956-; Takahashi, Rickey, 1957-; Tanaka, Doris, 1956-; Tom, Perry, 1958-; Tom, Steven, 1957-; Toyofuku, Lesley, 1956-; Wing, Linda; Wong, Danny Y., 1953-; Wong, Julie, 1956-; Woo, Tommy, 1956-; Woo, Wesley, 1957-; Yee, David, 1956-; Yee, Dorise, 1957-; Yee, Edward, 1957, and Asian Writers Project

Abstract

Excerpts from Sojourner IV - Pilipinos, anthology, Asian Writers Project, Berkeley High School, 2246 Milvia Street, Berkeley, California, USA, 1974 June.

Published
2019

9. Spectroscopy and kinetics of wild-type and mutant tyrosine hydroxylase: mechanistic insight into [O.sub.2] activation

Author

Chow, Marina S., Eser, Bekir E., Wilson, Samuel A., Hodgson, Keith O., Hedman, Britt, Fitzpatrick, Paul F., and Solomon, Edward I.

Subjects
Circular dichroism -- Usage, Hydroxylation -- Analysis, Magnetic fields -- Analysis, Mutation (Biology) -- Analysis, Tyrosine -- Chemical properties, X-ray spectroscopy -- Usage, Chemistry
Abstract

The geometric and electronic structure of the wild-type (WT) tyrosine hydroxylase (TH) and two mutants (S395A and E332A) and their interactions with substrates are examined by using x-ray absorption spectroscopy and variable-temperature-variable-field magnetic circular dichroism spectroscopy. All three forms of TH have undergone 6C -> 5C conversion with tyr + pterin, which is consistent with the general mechanistic strategy established for [O.sub.2]-activating nonheme iron enzymes.

Published
2009

10. Direct hydrogen-atom abstraction by activated bleomycin: An experimental and computational study

Author

Decker, Andrea, Chow, Marina S., Kemsley, Jyllian N., Lehnert, Nicolai, and Soloman, Edward I.

Subjects
Bleomycin -- Research, Density functionals -- Usage, Hydrogen -- Chemical properties, Chemistry
Abstract

Kinetic measurements in combination with density-functional calculations are used to study the reactivity of activated bleomycin (ABLM) and the mechanism of the initial attack on DNA. Direct H-atom abstraction by ABLM would generate a reactive [Fe.sup.IV]=O species, which would be capable of a second DNA strand cleavage and provide experimental and theoretical evidence for direct H-atom abstraction by ABLM. Study proposes an attractive mechanism for the role of ABLM in double-strand cleavage.

Published
2006

11. Sulfur K-Edge XAS and DFT Calculations on Nitrile Hydratase: Geometric and Electronic Structure of the Non-heme Iron Active Site

Author

Dey, Abhishek, primary, Chow, Marina, additional, Taniguchi, Kayoko, additional, Lugo-Mas, Priscilla, additional, Davin, Steven, additional, Maeda, Mizuo, additional, Kovacs, Julie A., additional, Odaka, Masafumi, additional, Hodgson, Keith O., additional, Hedman, Britt, additional, and Solomon, Edward I., additional

Published
2005
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