64 results on '"Chrisey DB"'
Search Results
2. Synthesis of Silicon and Germanium Oxide Nanostructures via Photonic Curing; a Facile Approach to Scale Up Fabrication.
- Author
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Khatoon N, Subedi B, and Chrisey DB
- Abstract
Silicon and Germanium oxide (SiO
x and GeOx ) nanostructures are promising materials for energy storage applications due to their potentially high energy density, large lithiation capacity (~10X carbon), low toxicity, low cost, and high thermal stability. This work reports a unique approach to achieving controlled synthesis of SiOx and GeOx nanostructures via photonic curing. Unlike conventional methods like rapid thermal annealing, quenching during pulsed photonic curing occurs rapidly (sub-millisecond), allowing the trapping of metastable states to form unique phases and nanostructures. We explored the possible underlying mechanism of photonic curing by incorporating laws of photophysics, photochemistry, and simulated temperature profile of thin film. The results show that photonic curing of spray coated 0.1 M molarity Si and Ge Acetyl Acetate precursor solution, at total fluence 80 J cm-2 can yield GeOx and SiOx nanostructures. The as-synthesized nanostructures are ester functionalized due to photoinitiated chemical reactions in thin film during photonic curing. Results also showed that nanoparticle size changes from ~48 nm to ~11 nm if overall fluence is increased by increasing the number of pulses. These results are an important contribution towards large-scale synthesis of the Ge and Si oxide nanostructured materials which is necessary for next-generation energy storage devices., (© 2024 The Authors. ChemistryOpen published by Wiley-VCH GmbH.)- Published
- 2024
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3. Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma.
- Author
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Yamaguchi E, Yao J, Aymond A, Chrisey DB, Nieman GF, Bates JHT, and Gaver DP
- Subjects
- Electric Impedance, Humans, Lung physiopathology, Pneumonia, Aspiration complications, Pneumonia, Aspiration physiopathology, Pulmonary Atelectasis physiopathology, Smoke Inhalation Injury etiology, Smoke Inhalation Injury physiopathology, COVID-19 complications, COVID-19 physiopathology, Pulmonary Atelectasis etiology, Respiratory Distress Syndrome, Ventilator-Induced Lung Injury complications, Ventilator-Induced Lung Injury prevention & control
- Abstract
Biophysical insults that either reduce barrier function (COVID-19, smoke inhalation, aspiration, and inflammation) or increase mechanical stress (surfactant dysfunction) make the lung more susceptible to atelectrauma. We investigate the susceptibility and time-dependent disruption of barrier function associated with pulmonary atelectrauma of epithelial cells that occurs in acute respiratory distress syndrome (ARDS) and ventilator-induced lung injury (VILI). This in vitro study was performed using Electric Cell-substrate Impedance Sensing (ECIS) as a noninvasive evaluating technique for repetitive stress stimulus/response on monolayers of the human lung epithelial cell line NCI-H441. Atelectrauma was mimicked through recruitment/derecruitment (RD) of a semi-infinite air bubble to the fluid-occluded micro-channel. We show that a confluent monolayer with a high level of barrier function is nearly impervious to atelectrauma for hundreds of RD events. Nevertheless, barrier function is eventually diminished, and after a critical number of RD insults, the monolayer disintegrates exponentially. Confluent layers with lower initial barrier function are less resilient. These results indicate that the first line of defense from atelectrauma resides with intercellular binding. After disruption, the epithelial layer community protection is diminished and atelectrauma ensues. ECIS may provide a platform for identifying damaging stimuli, ventilation scenarios, or pharmaceuticals that can reduce susceptibility or enhance barrier-function recovery.
- Published
- 2022
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4. Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization.
- Author
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Grumezescu V, Negut I, Cristescu R, Grumezescu AM, Holban AM, Iordache F, Chifiriuc MC, Narayan RJ, and Chrisey DB
- Subjects
- Anti-Bacterial Agents chemistry, Biofilms growth & development, Coated Materials, Biocompatible chemistry, Flavonoids chemistry, Lasers standards, Microbial Sensitivity Tests methods, Pseudomonas aeruginosa growth & development, Staphylococcus aureus growth & development, Surface Properties, Anti-Bacterial Agents pharmacology, Biofilms drug effects, Coated Materials, Biocompatible pharmacology, Drug Resistance, Microbial drug effects, Flavonoids pharmacology, Pseudomonas aeruginosa drug effects, Staphylococcus aureus drug effects
- Abstract
Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.
- Published
- 2021
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5. Nanostructured manganese oxides electrode with ultra-long lifetime for electrochemical capacitors.
- Author
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Gaire M, Liang K, Luo S, Subedi B, Adireddy S, Schroder K, Farnsworth S, and Chrisey DB
- Abstract
We describe the instantaneous fabrication of a highly porous three-dimensional (3D) nanostructured manganese oxides-reduced graphitic oxide (MnO
x -rGO) electrode by using a pulse-photonic processing technique. Such nanostructures facilitate the movement of ions/electrons and offer an extremely high surface area for the electrode/electrolyte interaction. The electrochemical performance was investigated by cyclic voltammetry (CV), galvanostatic charge-discharge (GCD) and electrochemical impedance spectroscopy (EIS) with 1 M KOH as the electrolyte. The as-prepared thin film electrode exhibits excellent electrochemical performance and an ultra-long lifetime by retaining 90% of the initial capacitance even after 100 000 GCD cycles at constant areal current density of 0.4 mA cm-2 . We attribute this excellent lifetime performance to the conductive reduced graphitic oxide, synergistic effects of carbon composite and the metal oxides, and the unique porous nanostructure. Such highly porous morphology also enhances the structural stability of the electrode by buffering the volume changes during the redox processes., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)- Published
- 2020
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6. Matrix-Assisted Pulsed laser Evaporation-deposited Rapamycin Thin Films Maintain Antiproliferative Activity.
- Author
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Cristescu R, Negut I, Visan AI, Nguyen AK, Sachan A, Goering PL, Chrisey DB, and Narayan RJ
- Abstract
Matrix-assisted pulsed laser evaporation (MAPLE) has many benefits over conventional methods (e.g., dip-coating, spin coating, and Langmuir-Blodgett dip-coating) for manufacturing coatings containing pharmacologic agents on medical devices. In particular, the thickness of the coating that is applied to the surface of the medical device can be tightly controlled. In this study, MAPLE was used to deposit rapamycin-polyvinylpyrrolidone (rapamycin-PVP) thin films onto silicon and borosilicate optical glass substrates. Alamar Blue and PicoGreen studies were used to measure the metabolic health and DNA content of L929 mouse fibroblasts as measures of viability and proliferation, respectively. The cells on the MAPLE-deposited rapamycin-PVP surfaces exhibited 70.6% viability and 53.7% proliferation compared to a borosilicate glass control. These data indicate that the antiproliferative properties of rapamycin were maintained after MAPLE deposition., (Copyright: © 2020 Cristescu, et al.)
- Published
- 2020
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7. Solvent-based Extrusion 3D Printing for the Fabrication of Tissue Engineering Scaffolds.
- Author
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Zhang B, Cristescu R, Chrisey DB, and Narayan RJ
- Abstract
Three-dimensional (3D) printing has been emerging as a new technology for scaffold fabrication to overcome the problems associated with the undesirable microstructure associated with the use of traditional methods. Solvent-based extrusion (SBE) 3D printing is a popular 3D printing method, which enables incorporation of cells during the scaffold printing process. The scaffold can be customized by optimizing the scaffold structure, biomaterial, and cells to mimic the properties of natural tissue. However, several technical challenges prevent SBE 3D printing from translation to clinical use, such as the properties of current biomaterials, the difficulties associated with simultaneous control of multiple biomaterials and cells, and the scaffold-to-scaffold variability of current 3D printed scaffolds. In this review paper, a summary of SBE 3D printing for tissue engineering (TE) is provided. The influences of parameters such as ink biomaterials, ink rheological behavior, cross-linking mechanisms, and printing parameters on scaffold fabrication are considered. The printed scaffold structure, mechanical properties, degradation, and biocompatibility of the scaffolds are summarized. It is believed that a better understanding of the scaffold fabrication process and assessment methods can improve the functionality of SBE-manufactured 3D printed scaffolds., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2020, Whioce Publishing Pte. Ltd.)
- Published
- 2020
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8. Novel Antimicrobial Surfaces to Defeat COVID-19 Transmission.
- Author
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Cristescu R, Narayan RJ, and Chrisey DB
- Abstract
Antimicrobial surface coatings function as a contact biocide and are extensively used to prevent the growth and transmission of pathogens on environmental surfaces. Currently, scientists and researchers are intensively working to develop antimicrobial, antiviral coating solutions that would efficiently impede/stop the contagion of COVID-19 via surface contamination. Herein we present a flavonoid-based antimicrobial surface coating fabricated by laser processing that has the potential to eradicate COVID-19 contact transmission. Quercetin-containing coatings showed better resistance to microbial colonization than antibiotic-containing ones., (© The Materials Research Society 2020.)
- Published
- 2020
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9. Chemotherapy-induced senescent cancer cells engulf other cells to enhance their survival.
- Author
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Tonnessen-Murray CA, Frey WD, Rao SG, Shahbandi A, Ungerleider NA, Olayiwola JO, Murray LB, Vinson BT, Chrisey DB, Lord CJ, and Jackson JG
- Subjects
- Animals, Cell Proliferation drug effects, Cell Survival drug effects, Humans, MCF-7 Cells, Mice, Tumor Cells, Cultured, Antibiotics, Antineoplastic pharmacology, Cellular Senescence drug effects, Doxorubicin pharmacology
- Abstract
In chemotherapy-treated breast cancer, wild-type p53 preferentially induces senescence over apoptosis, resulting in a persisting cell population constituting residual disease that drives relapse and poor patient survival via the senescence-associated secretory phenotype. Understanding the properties of tumor cells that allow survival after chemotherapy treatment is paramount. Using time-lapse and confocal microscopy to observe interactions of cells in treated tumors, we show here that chemotherapy-induced senescent cells frequently engulf both neighboring senescent or nonsenescent tumor cells at a remarkable frequency. Engulfed cells are processed through the lysosome and broken down, and cells that have engulfed others obtain a survival advantage. Gene expression analysis showed a marked up-regulation of conserved macrophage-like program of engulfment in chemotherapy-induced senescent cell lines and tumors. Our data suggest compelling explanations for how senescent cells persist in dormancy, how they manage the metabolically expensive process of cytokine production that drives relapse in those tumors that respond the worst, and a function for their expanded lysosomal compartment., (© 2019 Tonnessen-Murray et al.)
- Published
- 2019
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10. Novel application of the published kinase inhibitor set to identify therapeutic targets and pathways in triple negative breast cancer subtypes.
- Author
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Matossian MD, Elliott S, Hoang VT, Burks HE, Phamduy TB, Chrisey DB, Zuercher WJ, Drewry DH, Wells C, Collins-Burow B, and Burow ME
- Subjects
- Animals, Cell Line, Tumor, Cell Movement drug effects, Cell Transdifferentiation drug effects, Drug Screening Assays, Antitumor, Epithelial Cells drug effects, Epithelial Cells pathology, Humans, Mesoderm drug effects, Mesoderm pathology, Mice, Phenotype, Protein Kinase Inhibitors therapeutic use, Small Molecule Libraries pharmacology, Small Molecule Libraries therapeutic use, Triple Negative Breast Neoplasms enzymology, Triple Negative Breast Neoplasms pathology, Xenograft Model Antitumor Assays, Molecular Targeted Therapy, Protein Kinase Inhibitors pharmacology, Triple Negative Breast Neoplasms drug therapy
- Abstract
Triple negative breast cancers (TNBCs) have high recurrence and metastasis rates. Acquisition of a mesenchymal morphology and phenotype in addition to driving migration is a consequential process that promotes metastasis. Although some kinases are known to regulate a mesenchymal phenotype, the role for a substantial portion of the human kinome remains uncharacterized. Here we evaluated the Published Kinase Inhibitor Set (PKIS) and screened a panel of TNBC cell lines to evaluate the compounds' effects on a mesenchymal phenotype. Our screen identified 36 hits representative of twelve kinase inhibitor chemotypes based on reversal of the mesenchymal cell morphology, which was then prioritized to twelve compounds based on gene expression and migratory behavior analyses. We selected the most active compound and confirmed mesenchymal reversal on transcript and protein levels with qRT-PCR and Western Blot. Finally, we utilized a kinase array to identify candidate kinases responsible for the EMT reversal. This investigation shows the novel application to identify previously unrecognized kinase pathways and targets in acquisition of a mesenchymal TNBC phenotype that warrant further investigation. Future studies will examine specific roles of the kinases in mechanisms responsible for acquisition of the mesenchymal and/or migratory phenotype.
- Published
- 2017
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11. Printing amphotericin B on microneedles using matrix-assisted pulsed laser evaporation.
- Author
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Sachan R, Jaipan P, Zhang JY, Degan S, Erdmann D, Tedesco J, Vanderwal L, Stafslien SJ, Negut I, Visan A, Dorcioman G, Socol G, Cristescu R, Chrisey DB, and Narayan RJ
- Abstract
Transdermal delivery of amphotericin B, a pharmacological agent with activity against fungi and parasitic protozoa, is a challenge since amphotericin B exhibits poor solubility in aqueous solutions at physiologic pH values. In this study, we have used a laser-based printing approach known as matrix-assisted pulsed laser evaporation to print amphotericin B on the surfaces of polyglycolic acid microneedles that were prepared using a combination of injection molding and drawing lithography. In a modified agar disk diffusion assay, the amphotericin B-loaded microneedles showed concentration-dependent activity against the yeast Candida albicans . The results of this study suggest that matrix-assisted pulsed laser evaporation may be used to print amphotericin B and other drugs that have complex solubility issues on the surfaces of microneedles., Competing Interests: No conflict of interest was reported by the authors. We would like to acknowledge C Mooney (NCSU Analytical Instrumentation Facility) for his aid with electron microscopy, B Andersen for her aid with Fourier transform infared spectrosocpy (NCSU College of Textiles), P Strader (NCSU Analytical Instrumentation Facility) for his aid with nanoindentation, the US National Institutes of Health (Award # 1R21AI117748- 01A1), the US National Science Foundation (Award CMMI 1258536), and the US Office of Naval Research (Award # N00014-15-1-2323). This work was also supported by the National Program PN 4N/2016(1647- LAPLAS IV and a grant of Ministry of Research and Innovation, CNCS - UEFISCDI, Project Number PN- III-P4-ID-PCE-2016-0884, within PNCDI III., (Copyright: © 2017 Sachan, et al.)
- Published
- 2017
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12. Effects of living cells on the bioink printability during laser printing.
- Author
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Zhang Z, Xu C, Xiong R, Chrisey DB, and Huang Y
- Abstract
Laser-induced forward transfer has been a promising orifice-free bioprinting technique for the direct writing of three-dimensional cellular constructs from cell-laden bioinks. In order to optimize the printing performance, the effects of living cells on the bioink printability must be carefully investigated in terms of the ability to generate well-defined jets during the jet/droplet formation process as well as well-defined printed droplets on a receiving substrate during the jet/droplet deposition process. In this study, a time-resolved imaging approach has been implemented to study the jet/droplet formation and deposition processes when printing cell-free and cell-laden bioinks under different laser fluences. It is found that the jetting behavior changes from no material transferring to well-defined jetting with or without an initial bulgy shape to jetting with a bulgy shape/pluming/splashing as the laser fluence increases. Under desirable well-defined jetting, two impingement-based deposition and printing types are identified: droplet-impingement printing and jet-impingement printing with multiple breakups. Compared with cell-free bioink printing, the transfer threshold of the cell-laden bioink is higher while the jet velocity, jet breakup length, and printed droplet size are lower, shorter, and smaller, respectively. The addition of living cells transforms the printing type from jet-impingement printing with multiple breakups to droplet-impingement printing. During the printing of cell-laden bioinks, two non-ideal jetting behaviors, a non-straight jet with a non-straight trajectory and a straight jet with a non-straight trajectory, are identified mainly due to the local nonuniformity and nonhomogeneity of cell-laden bioinks.
- Published
- 2017
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13. Study of gelatin as an effective energy absorbing layer for laser bioprinting.
- Author
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Xiong R, Zhang Z, Chai W, Chrisey DB, and Huang Y
- Subjects
- Animals, Bioprinting instrumentation, Cell Survival physiology, DNA Damage, Equipment Design, Lasers, Mice, NIH 3T3 Cells, Tissue Engineering instrumentation, Bioprinting methods, Gelatin chemistry, Printing, Three-Dimensional, Tissue Engineering methods
- Abstract
Laser-induced forward transfer printing, also commonly known as laser printing, has been widely implemented for three-dimensional bioprinting due to its unique orifice-free nature during printing. However, the printing quality has the potential to be further improved for various laser bioprinting applications. The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage. The gelatin EAL is applied between the quartz support and the coating of build material, which is to be printed. Printing quality can be improved by EAL-assisted laser printing when using various alginate solutions (1%, 2%, and 4%) and cell-laden bioinks (2% alginate and 5 × 10
6 cells ml-1 in cell culture medium). The required laser fluence is also reduced due to a higher absorption coefficient of gelatin gel, in particular when to achieve the best printing type/quality. The post-printing cell viability is improved by ∼10% and DNA double-strand breaks are reduced by ∼50%. For all the build materials investigated, the gelatin EAL helps reduce the droplet size and average jet velocity.- Published
- 2017
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14. Directed self-assembly software for single cell deposition.
- Author
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Sklare SC, Richey WL, Vinson BT, and Chrisey DB
- Abstract
Laser direct-write (LDW) bioprinting methods offer a diverse set of tools to design experiments, fabricate tissue constructs and to cellular microenvironments all in a CAD/CAM manner. To date, we have just scratched the surface of the system's potential and for LDW to be utilized to its fullest, there are many distinct hardware and software components that must be integrated and communicate seamlessly. In this perspective article, we detail the development of novel graphical user interface (GUI) software to improve LDW capability and functionality. The main modules in the control software correspond to cell transfer, microbead fabrication, and micromachining. The modules make the control of each of these features, and the management of printing programs that utilize one or more features, to be facile. The software also addresses problems related to construct scale-up, print speed, experimental conditions, and management of sensor data. The control software and possibilities for integrated sensor data are presented., Competing Interests: No conflict of interest was reported by the authors., (Copyright: © 2017 Sklare, et al.)
- Published
- 2017
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15. Laser direct-write based fabrication of a spatially-defined, biomimetic construct as a potential model for breast cancer cell invasion into adipose tissue.
- Author
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Vinson BT, Phamduy TB, Shipman J, Riggs B, Strong AL, Sklare SC, Murfee WL, Burow ME, Bunnell BA, Huang Y, and Chrisey DB
- Subjects
- Adipocytes metabolism, Alginates chemistry, Bioprinting, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cell Line, Tumor, Cell Movement, Coculture Techniques, Collagen chemistry, Computer-Aided Design, Epithelial-Mesenchymal Transition, Female, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Hydrogels chemistry, MCF-7 Cells, Microscopy, Electron, Scanning, Adipocytes cytology, Biomimetics, Lasers, Models, Biological, Tissue Scaffolds chemistry
- Abstract
Epithelial-adipose interaction is an integral step in breast cancer cell invasion and progression towards lethal metastatic disease. Understanding the physiological contribution of obesity, a major contributor to breast cancer risk and negative prognosis in post-menopausal patients, on cancer cell invasion requires detailed co-culture constructs that reflect mammary microarchitecture. Using laser direct-write, a laser-based CAD/CAM bioprinting technique, we have demonstrated the ability to construct breast cancer cell-laden hydrogel microbeads into spatially defined patterns in hydrogel matrices containing differentiated adipocytes. Z-stack imaging confirmed the three-dimensional nature of the constructs, as well as incorporation of cancer cell-laden microbeads into the adipose matrix. Preliminary data was gathered to support the construct as a potential model for breast cancer cell invasion into adipose tissue. MCF-7 and MDA-MB-231 breast cancer cell invasion was tracked over 2 weeks in an optically transparent hydrogel scaffold in the presence of differentiated adipocytes obtained from normal weight or obese patient tissue. Our model successfully integrates adipocytes and gives us the potential to study cellular and tissue-level interactions towards the early detection of cancer cell invasion into adipose tissue.
- Published
- 2017
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16. Laser Direct-Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration.
- Author
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Burks HE, Phamduy TB, Azimi MS, Saksena J, Burow ME, Collins-Burow BM, Chrisey DB, and Murfee WL
- Subjects
- Animals, Bioprinting, Humans, Rats, Time-Lapse Imaging, Cell Movement, Lasers, Models, Biological, Neoplasms pathology, Organ Specificity
- Abstract
Investigation into the mechanisms driving cancer cell behavior and the subsequent development of novel targeted therapeutics requires comprehensive experimental models that mimic the complexity of the tumor microenvironment. Recently, our laboratories have combined a novel tissue culture model and laser direct-write, a form of bioprinting, to spatially position single or clustered cancer cells onto ex vivo microvascular networks containing blood vessels, lymphatic vessels, and interstitial cell populations. Herein, we highlight this new model as a tool for quantifying cancer cell motility and effects on angiogenesis and lymphangiogenesis in an intact network that matches the complexity of a real tissue. Application of our proposed methodology offers an innovative ex vivo tissue perspective for evaluating the effects of gene expression and targeted molecular therapies on cancer cell migration and invasion. J. Cell. Physiol. 231: 2333-2338, 2016. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)
- Published
- 2016
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17. Isolated node engineering of neuronal systems using laser direct write.
- Author
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Curley JL, Sklare SC, Bowser DA, Saksena J, Moore MJ, and Chrisey DB
- Subjects
- Animals, Cells, Cultured, Cellular Microenvironment physiology, Nerve Net cytology, Nerve Net physiology, Rats, Rats, Long-Evans, Surface Properties radiation effects, Ganglia, Spinal cytology, Lasers, Molecular Imprinting methods, Neurons cytology, Neurons physiology, Tissue Engineering methods
- Abstract
Current limitations to the engineering of ex vivo and in vitro neural environments are hampering the ability to understand underlying neurophysiology. High levels of spatial specificity, reproducibility and viability have been previously reported using laser direct write (LDW) to print cells. However, despite the significant need no one has yet reported laser assisted printing of primary mammalian neuronal cells, an inherently sensitive but critically important population. Herein, we describe the use of LDW to reproducibly and accurately pattern viable dorsal root ganglion (DRG) neurons and supportive cells capable of neural outgrowth and network formation. Our demonstrated ability to engineer and control distinct micro-environmental components unlocks the potential for high throughput experiments to both understand underlying physiology and investigate therapeutic interventions.
- Published
- 2016
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18. Freeform drop-on-demand laser printing of 3D alginate and cellular constructs.
- Author
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Xiong R, Zhang Z, Chai W, Huang Y, and Chrisey DB
- Subjects
- Animals, Cell Survival, Gels, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Image Processing, Computer-Assisted, Mice, NIH 3T3 Cells, Tissue Scaffolds chemistry, Alginates chemistry, Lasers, Printing, Three-Dimensional
- Abstract
Laser printing is an orifice-free printing approach and has been investigated for the printing of two-dimensional patterns and simple three-dimensional (3D) constructs. To demonstrate the potential of laser printing as an effective bioprinting technique, both straight and Y-shaped tubes have been freeform printed using two different bioinks: 8% alginate solution and 2% alginate-based mouse fibroblast suspension. It has been demonstrated that 3D cellular tubes, including constructs with bifurcated overhang structures, can be adequately fabricated under optimal printing conditions. The post-printing cell viabilities immediately after printing as well as after 24 h incubation are above 60% for printed straight and Y-shaped fibroblast tubes. During fabrication, overhang and spanning structures can be printed using a dual-purpose crosslinking solution, which also functions as a support material. The advancement distance of gelation reaction front after a cycle time of the receiving platform downward motion should be estimated for experimental planning. The optimal downward movement step size of receiving platform should be chosen to be equal to the height of ungelled portion of a previously printed layer.
- Published
- 2015
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19. In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles.
- Author
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Patil US, Adireddy S, Jaiswal A, Mandava S, Lee BR, and Chrisey DB
- Subjects
- Animals, Cell Death drug effects, Humans, Ferric Compounds metabolism, Ferric Compounds toxicity, Metal Nanoparticles toxicity
- Abstract
Increasing biomedical applications of iron oxide nanoparticles (IONPs) in academic and commercial settings have alarmed the scientific community about the safety and assessment of toxicity profiles of IONPs. The great amount of diversity found in the cytotoxic measurements of IONPs points toward the necessity of careful characterization and quantification of IONPs. The present document discusses the major developments related to in vitro and in vivo toxicity assessment of IONPs and its relationship with the physicochemical parameters of IONPs. Major discussion is included on the current spectrophotometric and imaging based techniques used for quantifying, and studying the clearance and biodistribution of IONPs. Several invasive and non-invasive quantification techniques along with the pitfalls are discussed in detail. Finally, critical guidelines are provided to optimize the design of IONPs to minimize the toxicity.
- Published
- 2015
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20. Printing cancer cells into intact microvascular networks: a model for investigating cancer cell dynamics during angiogenesis.
- Author
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Phamduy TB, Sweat RS, Azimi MS, Burow ME, Murfee WL, and Chrisey DB
- Subjects
- Animals, Cell Movement, Cell Separation methods, Equipment Design, Equipment Failure Analysis, Humans, MCF-7 Cells, Mesentery physiopathology, Neoplasm Invasiveness, Printing, Three-Dimensional statistics & numerical data, Rats, Cell Separation instrumentation, Microvessels physiopathology, Neoplasms, Experimental pathology, Neoplasms, Experimental physiopathology, Neovascularization, Pathologic pathology, Neovascularization, Pathologic physiopathology
- Abstract
While cancer cell invasion and metastasis are dependent on cancer cell-stroma, cancer cell-blood vessel, and cancer cell-lymphatic vessel interactions, our understanding of these interactions remain largely unknown. A need exists for physiologically-relevant models that more closely mimic the complexity of cancer cell dynamics in a real tissue environment. The objective of this study was to combine laser-based cell printing and tissue culture methods to create a novel ex vivo model in which cancer cell dynamics can be tracked during angiogenesis in an intact microvascular network. Laser direct-write (LDW) was utilized to reproducibly deposit breast cancer cells (MDA-MB-231 and MCF-7) and fibroblasts into spatially-defined patterns on cultured rat mesenteric tissues. In addition, heterogeneous patterns containing co-printed MDA-MB-231/fibroblasts or MDA-MB-231/MCF-7 cells were generated for fibroblast-directed and collective cell invasion models. Printed cells remained viable and the cells retained the ability to proliferate in serum-rich media conditions. Over a culture period of five days, time-lapse imaging confirmed fibroblast and MDA-MB-231 cell migration within the microvascular networks. Confocal microscopy indicated that printed MDA-MB-231 cells infiltrated the tissue thickness and were capable of interacting with endothelial cells. Angiogenic network growth in tissue areas containing printed cancer cells was characterized by significantly increased capillary sprouting compared to control tissue areas containing no printed cells. Our results establish an innovative ex vivo experimental platform that enables time-lapse evaluation of cancer cell dynamics during angiogenesis within a real microvascular network scenario.
- Published
- 2015
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21. Click-In Ferroelectric Nanoparticles for Dielectric Energy Storage.
- Author
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Riggs BC, Elupula R, Rehm C, Adireddy S, Grayson SM, and Chrisey DB
- Abstract
Polymer-ceramic nanocomposites have been thoroughly investigated previously for high energy storage devices. However, the increase in performance of these nanocomposites has proven to be significantly lower than predicted values. Through surface functionalization of high dielectric constant nanoparticles (NP), the flaws that reduce composite performance can be eliminated to form high energy density composite materials. Functionalization methods utilize high throughput printing and curing techniques (i.e., inkjet printing and xenon flash lamp curing) that are crucial for rapid adoption into industrial production. (Ba,Ca) (Zr,Ti)O3 NPs (50 nm) are synthesized through the solvothermal method and functionalized with alkene terminated methoxysilanes. A thiol-ene monomer ink system, PTD3 [pentaerythritol tetrakis (3-mercaptopropionate) (PEMP, P), 1,3-Diisopropenylbenzene (DPB, D), 2,4,6-Triallyloxy-1,3,5-triazine (TOTZ, T)], is used as a high breakdown polymer matrix. Neat polymer, alkene terminated NP-polymer composites, and hydrophilic, TBAOH functionalized NP-polymer composites were spin coated onto both copper laminated glass slides and printed onto copper substrates in 1 cm(2) patterns for testing. Alkene functionalized NPs increased the breakdown strength by ∼38% compared to the nonfunctionalized NPs. Functionalized NPs increased both the breakdown strength and dielectric constant compared to the neat polymer, increasing the energy density nearly 3-fold from 13.3 to 36.1 J·cm(-3).
- Published
- 2015
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22. Time-Resolved Imaging Study of Jetting Dynamics during Laser Printing of Viscoelastic Alginate Solutions.
- Author
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Zhang Z, Xiong R, Mei R, Huang Y, and Chrisey DB
- Subjects
- Biocompatible Materials, Bioprinting instrumentation, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Ink, Lasers, Printing instrumentation, Alginates chemistry, Bioprinting methods, Printing methods, Viscoelastic Substances chemistry
- Abstract
Matrix-assisted pulsed-laser evaporation direct-write (MAPLE DW) has been successfully implemented as a promising laser printing technology for various fabrication applications, in particular, three-dimensional bioprinting. Since most bioinks used in bioprinting are viscoelastic, it is of importance to understand the jetting dynamics during the laser printing of viscoelastic fluids in order to control and optimize the laser printing performance. In this study, MAPLE DW was implemented to study the jetting dynamics during the laser printing of representative viscoelastic alginate bioinks and evaluate the effects of operating conditions (e.g., laser fluence) and material properties (e.g., alginate concentration) on the jet formation performance. Through a time-resolved imaging approach, it is found that when the laser fluence increases or the alginate concentration decreases, the jetting behavior changes from no material transferring to well-defined jetting to well-defined jetting with an initial bulgy shape to jetting with a bulgy shape to pluming/splashing. For the desirable well-defined jetting regimes, as the laser fluence increases, the jet velocity and breakup length increase while the breakup time and primary droplet size decrease. As the alginate concentration increases, the jet velocity and breakup length decrease while the breakup time and primary droplet size increase. In addition, Ohnesorge, elasto-capillary, and Weber number based phase diagrams are presented to better appreciate the dependence of jetting regimes on the laser fluence and alginate concentration.
- Published
- 2015
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23. Fabrication of magnetite-based core-shell coated nanoparticles with antibacterial properties.
- Author
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Grumezescu AM, Cristescu R, Chifiriuc MC, Dorcioman G, Socol G, Mihailescu IN, Mihaiescu DE, Ficai A, Vasile OR, Enculescu M, and Chrisey DB
- Subjects
- Adsorption, Biofilms drug effects, Cell Line, Tumor, Ferric Compounds chemistry, Humans, Lasers, Magnetite Nanoparticles ultrastructure, Microbial Sensitivity Tests, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa physiology, Sodium Dodecyl Sulfate chemistry, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus drug effects, Staphylococcus aureus physiology, Volatilization, X-Ray Diffraction, Anti-Bacterial Agents pharmacology, Magnetite Nanoparticles chemistry, Microtechnology methods
- Abstract
We report the fabrication of biofunctionalized magnetite core/sodium lauryl sulfate shell/antibiotic adsorption-shell nanoparticles assembled thin coatings by matrix assisted pulsed laser evaporation for antibacterial drug-targeted delivery. Magnetite nanoparticles have been synthesized and subsequently characterized by transmission electron microscopy and x-ray diffraction. The obtained thin coatings have been investigated by FTIR and scanning electron microscope, and tested by in vitro biological assays, for their influence on in vitro bacterial biofilm development and cytotoxicity on human epidermoid carcinoma (HEp2) cells.
- Published
- 2015
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- View/download PDF
24. Alginate gelation-induced cell death during laser-assisted cell printing.
- Author
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Gudapati H, Yan J, Huang Y, and Chrisey DB
- Subjects
- 3T3 Cells, Alginates chemistry, Animals, Calcium Chloride adverse effects, Calcium Chloride chemistry, Glucuronic Acid adverse effects, Glucuronic Acid chemistry, Hexuronic Acids adverse effects, Hexuronic Acids chemistry, Mice, Microspheres, Tissue Engineering instrumentation, Alginates adverse effects, Bioprinting instrumentation, Cell Death radiation effects, Fibroblasts cytology, Lasers adverse effects
- Abstract
Modified laser-induced forward transfer has emerged as a promising bioprinting technique. Depending on the operating conditions and cell properties, laser cell printing may cause cell injury and even death, which should be carefully elucidated for it to be a viable technology. This study has investigated the effects of alginate gelation, gelation time, alginate concentration, and laser fluence on the post-transfer cell viability of NIH 3T3 fibroblasts. Sodium alginate and calcium chloride are used as the gel precursor and gel reactant solution to form cell-laden alginate microspheres. It is found that the effects of gelation depend on the duration of gelation. Two-minute gelation is observed to increase the cell viability after 24 h incubation, mainly due to the protective cushion effect of the forming gel membrane during droplet landing. Despite the cushion effect from 10 min gelation, it is observed that the cell viability decreases after 24 h incubation because of the forming thick gel membrane that reduces nutrient and oxygen diffusion from the culture medium. In addition, the cell viability after 24 h incubation decreases as the laser fluence or alginate concentration increases.
- Published
- 2014
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25. Suppression of triple-negative breast cancer metastasis by pan-DAC inhibitor panobinostat via inhibition of ZEB family of EMT master regulators.
- Author
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Rhodes LV, Tate CR, Segar HC, Burks HE, Phamduy TB, Hoang V, Elliott S, Gilliam D, Pounder FN, Anbalagan M, Chrisey DB, Rowan BG, Burow ME, and Collins-Burow BM
- Subjects
- Animals, Antineoplastic Agents pharmacology, Apoptosis drug effects, Breast pathology, Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Epithelial-Mesenchymal Transition drug effects, Female, Homeodomain Proteins biosynthesis, Humans, MCF-7 Cells, Mice, Mice, SCID, Neoplasm Invasiveness pathology, Neoplasm Metastasis drug therapy, Panobinostat, Repressor Proteins biosynthesis, Transcription Factors biosynthesis, Xenograft Model Antitumor Assays, Zinc Finger E-box Binding Homeobox 2, Zinc Finger E-box-Binding Homeobox 1, Histone Deacetylase Inhibitors pharmacology, Homeodomain Proteins antagonists & inhibitors, Hydroxamic Acids pharmacology, Indoles pharmacology, Repressor Proteins antagonists & inhibitors, Transcription Factors antagonists & inhibitors, Triple Negative Breast Neoplasms drug therapy
- Abstract
Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer subtype that lacks effective targeted therapies. The epithelial-to-mesenchymal transition (EMT) is a key contributor in the metastatic process. We previously showed the pan-deacetylase inhibitor LBH589 induces CDH1 expression in TNBC cells, suggesting regulation of EMT. The purpose of this study was to examine the effects of LBH589 on the metastatic qualities of TNBC cells and the role of EMT in this process. A panel of breast cancer cell lines (MCF-7, MDA-MB-231, and BT-549), drugged with LBH589, was examined for changes in cell morphology, migration, and invasion in vitro. The effect on in vivo metastasis was examined using immunofluorescent staining of lung sections. EMT gene expression profiling was used to determine LBH589-induced changes in TNBC cells. ZEB overexpression studies were conducted to validate requirement of ZEB in LBH589-mediated proliferation and tumorigenesis. Our results indicate a reversal of EMT by LBH589 as demonstrated by altered morphology and altered gene expression in TNBC. LBH589 was shown to be a more potent inhibitor of EMT than other HDAC inhibitors, SAHA and TMP269. Additionally, we found that LBH589 inhibits metastasis of MDA-MB-231 cells in vivo. These effects of LBH589 were mediated in part by inhibition of ZEB, as overexpression of ZEB1 or ZEB2 mitigated the effects of LBH589 on MDA-MB-231 EMT-associated gene expression, migration, invasion, CDH1 expression, and tumorigenesis. These data indicate therapeutic potential of LBH589 in targeting EMT and metastasis of TNBC.
- Published
- 2014
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26. Generating size-controlled embryoid bodies using laser direct-write.
- Author
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Dias AD, Unser AM, Xie Y, Chrisey DB, and Corr DT
- Subjects
- Animals, Cell Differentiation, Cell Line, Cellular Microenvironment, Lasers, Mice, Particle Size, Bioprinting methods, Cell Culture Techniques methods, Embryoid Bodies cytology
- Abstract
Embryonic stem cells (ESCs) have the potential to self-renew and differentiate into any specialized cell type. One common method to differentiate ESCs in vitro is through embryoid bodies (EBs), three-dimensional cellular aggregates that spontaneously self-assemble and generally express markers for the three germ layers, endoderm, ectoderm, and mesoderm. It has been previously shown that both EB size and 2D colony size each influence differentiation. We hypothesized that we could control the size of the EB formed by mouse ESCs (mESCs) by using a cell printing method, laser direct-write (LDW), to control both the size of the initial printed colony and the local cell density in printed colonies. After printing mESCs at various printed colony sizes and printing densities, two-way ANOVAs indicated that the EB diameter was influenced by printing density after three days (p = 0.0002), while there was no effect of the printed colony diameter on the EB diameter at the same timepoint (p = 0.74). There was no significant interaction between these two factors. Tukey's honestly significant difference test showed that high-density colonies formed significantly larger EBs, suggesting that printed mESCs quickly aggregate with nearby cells. Thus, EBs can be engineered to a desired size by controlling printing density, which will influence the design of future differentiation studies. Herein, we highlight the capacity of LDW to control the local cell density and colony size independently, at prescribed spatial locations, potentially leading to better stem cell maintenance and directed differentiation.
- Published
- 2014
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27. Single-step laser-based fabrication and patterning of cell-encapsulated alginate microbeads.
- Author
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Kingsley DM, Dias AD, Chrisey DB, and Corr DT
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Alginates chemistry, Bioprinting methods, Capsules, Cell Survival physiology, Microspheres
- Abstract
Alginate can be used to encapsulate mammalian cells and for the slow release of small molecules. Packaging alginate as microbead structures allows customizable delivery for tissue engineering, drug release, or contrast agents for imaging. However, state-of-the-art microbead fabrication has a limited range in achievable bead sizes, and poor control over bead placement, which may be desired to localize cellular signaling or delivery. Herein, we present a novel, laser-based method for single-step fabrication and precise planar placement of alginate microbeads. Our results show that bead size is controllable within 8%, and fabricated microbeads can remain immobilized within 2% of their target placement. Demonstration of this technique using human breast cancer cells shows that cells encapsulated within these microbeads survive at a rate of 89.6%, decreasing to 84.3% after five days in culture. Infusing rhodamine dye into microbeads prior to fluorescent microscopy shows their 3D spheroidal geometry and the ability to sequester small molecules. Microbead fabrication and patterning is compatible with conventional cellular transfer and patterning by laser direct-write, allowing location-based cellular studies. While this method can also be used to fabricate microbeads en masse for collection, the greatest value to tissue engineering and drug delivery studies and applications lies in the pattern registry of printed microbeads.
- Published
- 2013
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28. Engineering cellular fibers for musculoskeletal soft tissues using directed self-assembly.
- Author
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Schiele NR, Koppes RA, Chrisey DB, and Corr DT
- Subjects
- Cadherins metabolism, Cells, Cultured, Fibroblasts cytology, Fibroblasts metabolism, Fibronectins chemistry, Humans, Immunohistochemistry, Muscle, Skeletal cytology, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
Engineering strategies guided by developmental biology may enhance and accelerate in vitro tissue formation for tissue engineering and regenerative medicine applications. In this study, we looked toward embryonic tendon development as a model system to guide our soft tissue engineering approach. To direct cellular self-assembly, we utilized laser micromachined, differentially adherent growth channels lined with fibronectin. The micromachined growth channels directed human dermal fibroblast cells to form single cellular fibers, without the need for a provisional three-dimensional extracellular matrix or scaffold to establish a fiber structure. Therefore, the resulting tissue structure and mechanical characteristics were determined solely by the cells. Due to the self-assembly nature of this approach, the growing fibers exhibit some key aspects of embryonic tendon development, such as high cellularity, the rapid formation (within 24 h) of a highly organized and aligned cellular structure, and the expression of cadherin-11 (indicating direct cell-to-cell adhesions). To provide a dynamic mechanical environment, we have also developed and characterized a method to apply precise cyclic tensile strain to the cellular fibers as they develop. After an initial period of cellular fiber formation (24 h postseeding), cyclic strain was applied for 48 h, in 8-h intervals, with tensile strain increasing from 0.7% to 1.0%, and at a frequency of 0.5 Hz. Dynamic loading dramatically increased cellular fiber mechanical properties with a nearly twofold increase in both the linear region stiffness and maximum load at failure, thereby demonstrating a mechanism for enhancing cellular fiber formation and mechanical properties. Tissue engineering strategies, designed to capture key aspects of embryonic development, may provide unique insight into accelerated maturation of engineered replacement tissue, and offer significant advances for regenerative medicine applications in tendon, ligament, and other fibrous soft tissues.
- Published
- 2013
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29. Generation of Ag-Ag(2)O complex nanostructures by excimer laser ablation of Ag in water.
- Author
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Yan Z, Bao R, and Chrisey DB
- Abstract
Pulsed laser ablation in liquid (PLAL) has been well established as a facile method to produce nanoparticles from bulk materials, but it is still insufficient for fabricating anisotropic and complex nanostructures, especially without the use of surfactants. Here, we demonstrate that silver (Ag) nanosheets can be produced by pulsed excimer laser ablation of bulk Ag in water via laser re-processing of the laser-produced primary clusters. We also show that by combining PLAL and drop evaporation, rice-shaped Ag-Ag(2)O particles and their assemblies can be generated on Si substrates, because the interior flow of an evaporating colloidal drop could redistribute the laser-produced primary clusters, which results in the formation of complex nanostructures. These results show that PLAL is able to fabricate novel micro-/nanostructures while keeping its merit of "clean" fabrication.
- Published
- 2013
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30. Laser-assisted printing of alginate long tubes and annular constructs.
- Author
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Yan J, Huang Y, and Chrisey DB
- Subjects
- Biocompatible Materials chemical synthesis, Bioprinting instrumentation, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Lasers, Surface Properties, Alginates chemistry, Biocompatible Materials chemistry, Bioprinting methods, Tissue Engineering instrumentation
- Abstract
Laser-assisted printing such as laser-induced forward transfer has been well studied to pattern or fabricate two-dimensional constructs. In particular, laser printing has found increasing biomedical applications as an orifice-free cell and organ printing approach, especially for highly viscous biomaterials and biological materials. Unfortunately, there have been very few studies on the efficacy of three-dimensional printing performance of laser printing. This study has investigated the feasibility of laser tube printing and the effects of sodium alginate concentration and operating conditions such as the laser fluence and laser spot size on the printing quality during laser-assisted printing of alginate annular constructs (short tubes) with a nominal diameter of 3 mm. It is found that highly viscous materials such as alginate can be printed into well-defined long tubes and annular constructs. The tube wall thickness and tube outer diameter decrease with the sodium alginate concentration, while they first increase, then decrease and finally increase again with the laser fluence. The sodium alginate concentration dominates if the laser fluence is low, and the laser fluence dominates if the sodium alginate concentration is low.
- Published
- 2013
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31. Functionalized magnetite silica thin films fabricated by MAPLE with antibiofilm properties.
- Author
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Mihaiescu DE, Cristescu R, Dorcioman G, Popescu CE, Nita C, Socol G, Mihailescu IN, Grumezescu AM, Tamas D, Enculescu M, Negrea RF, Ghica C, Chifiriuc C, Bleotu C, and Chrisey DB
- Subjects
- Anti-Bacterial Agents pharmacology, Drug Carriers chemical synthesis, Drug Delivery Systems methods, Lasers, Pseudomonas aeruginosa drug effects, Silicon Dioxide chemistry, Staphylococcus aureus drug effects, Anti-Bacterial Agents chemistry, Biofilms drug effects, Drug Carriers chemistry, Drug Delivery Systems instrumentation, Magnetite Nanoparticles chemistry, Pseudomonas aeruginosa physiology, Staphylococcus aureus physiology
- Abstract
We report on the fabrication of magnetite/salicylic acid/silica shell/antibiotics (Fe(3)O(4)/SA/SiO(2)/ATB) thin films by matrix-assisted pulsed laser evaporation (MAPLE) to inert substrates. Fe(3)O(4)-based powder have been synthesized and investigated by XRD and TEM. All thin films were studied by FTIR, SEM and in vitro biological assays using Staphylococcus aureus and Pseudomonas aeruginosa reference strains, as well as eukaryotic HEp-2 cells. The influence of the obtained nanosystems on the microbial biofilm development as well as their biocompatibility has been assessed. For optimum deposition conditions, we obtained uniform adherent films with the composition identical with the raw materials. Fe(3)O(4)/SA/SiO(2)/ATB thin films had an inhibitory activity on the ability of microbial strains to initiate and develop mature biofilms, in a strain- and antibiotic-dependent manner. These magnetite silica thin films are promising candidates for the development of novel materials designed for the inhibition of medical biofilms formed by different pathogenic agents on common substrates, frequently implicated in the etiology of chronic and hard to treat infections.
- Published
- 2013
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32. Relaxor-ferroelectric superlattices: high energy density capacitors.
- Author
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Ortega N, Kumar A, Scott JF, Chrisey DB, Tomazawa M, Kumari S, Diestra DG, and Katiyar RS
- Abstract
We report the breakdown electric field and energy density of laser ablated BaTiO(3)/Ba((1-x))Sr(x)TiO(3) (x = 0.7) (BT/BST) relaxor-ferroelectric superlattices (SLs) grown on (100) MgO single crystal substrates. The dielectric constant shows a frequency dispersion below the dielectric maximum temperature (T(m)) with a merger above T(m) behaving similarly to relaxors. It also follows the basic criteria of relaxor ferroelectrics such as low dielectric loss over wide temperature and frequency, and 50 K shift in T(m) with change in probe frequency; the loss peaks follow a similar trend to the dielectric constant except that they increase with increase in frequency (~40 kHz), and satisfy the nonlinear Vogel-Fulcher relation. Well-saturated ferroelectric hysteresis and 50-80% dielectric saturation are observed under high electric field (~1.65 MV cm(-1)). The superlattices demonstrate an 'in-built' field in as grown samples at low probe frequency (<1 kHz), whereas it becomes more symmetric and centered with increase in the probe frequency system (>1 kHz) which rules out the effect of any space charge and interfacial polarization. The P-E loops show around 12.24 J cm(-3) energy density within the experimental limit, but extrapolation of this data suggests that the potential energy density could reach 46 J cm(-3). The current density versus applied electric field indicates an exceptionally high breakdown field (5.8-6.0 MV cm(-1)) and low current density (~10-25 mA cm(-2)) near the breakdown voltage. The current-voltage characteristics reveal that the space charge limited conduction mechanism prevails at very high voltage.
- Published
- 2012
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33. Laser direct-write of single microbeads into spatially-ordered patterns.
- Author
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Phamduy TB, Raof NA, Schiele NR, Yan Z, Corr DT, Huang Y, Xie Y, and Chrisey DB
- Subjects
- Adsorption, Alginates chemistry, Animals, Cell Adhesion, Cell Survival, Embryonic Stem Cells cytology, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Hydrogel, Polyethylene Glycol Dimethacrylate chemistry, Mice, Microscopy, Phase-Contrast, Particle Size, Polystyrenes chemistry, Biotechnology methods, Cell Culture Techniques methods, Lasers, Microspheres
- Abstract
Fabrication of heterogeneous microbead patterns on a bead-by-bead basis promotes new opportunities for sensors, lab-on-a-chip technology and cell-culturing systems within the context of customizable constructs. Laser direct-write (LDW) was utilized to target and deposit solid polystyrene and stem cell-laden alginate hydrogel beads into computer-programmed patterns. We successfully demonstrated single-bead printing resolution and fabricated spatially-ordered patterns of microbeads. The probability of successful microbead transfer from the ribbon surface increased from 0 to 80% with decreasing diameter of 600 to 45 µm, respectively. Direct-written microbeads retained spatial pattern registry, even after 10 min of ultrasonication treatment. SEM imaging confirmed immobilization of microbeads. Viability of cells encapsulated in transferred hydrogel microbeads achieved 37 ± 11% immediately after the transfer process, whereas randomly-patterned pipetted control beads achieved a viability of 51 ± 25%. Individual placement of >10 µm diameter microbeads onto planar surfaces has previously been unattainable. We have demonstrated LDW as a valuable tool for the patterning of single, micrometer-diameter beads into spatially-ordered patterns.
- Published
- 2012
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34. Fabrication and formation mechanism of hollow MgO particles by pulsed excimer laser ablation of Mg in liquid.
- Author
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Yan Z, Bao R, Busta CM, and Chrisey DB
- Abstract
We report on the formation of hollow MgO particles by excimer laser ablation of bulk Mg in water and aqueous solutions of sodium dodecyl sulfate (SDS) and sodium citrate (SC). Lamellar nanostructures of Mg(OH)(2) also formed in water, but the formation could be avoided by the addition of SDS or SC. Laser ablation produced not only Mg species that were oxidized into MgO and Mg(OH)(2) in water, but also cavitation bubbles. The bubble interfaces trapped the MgO nanoparticles to decrease the surface free energy of the system, finally resulting in hollow particles.
- Published
- 2011
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- View/download PDF
35. Gelatin-based laser direct-write technique for the precise spatial patterning of cells.
- Author
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Schiele NR, Chrisey DB, and Corr DT
- Subjects
- Animals, Cell Shape, Cell Survival, DNA Damage, Dermis cytology, Fibroblasts metabolism, Fibronectins biosynthesis, Fluorescent Antibody Technique, Histones metabolism, Humans, Microscopy, Atomic Force, Phosphorylation, Sus scrofa, Time Factors, Cell Culture Techniques methods, Fibroblasts cytology, Gelatin chemistry, Lasers
- Abstract
Laser direct-writing provides a method to pattern living cells in vitro, to study various cell-cell interactions, and to build cellular constructs. However, the materials typically used may limit its long-term application. By utilizing gelatin coatings on the print ribbon and growth surface, we developed a new approach for laser cell printing that overcomes the limitations of Matrigel™. Gelatin is free of growth factors and extraneous matrix components that may interfere with cellular processes under investigation. Gelatin-based laser direct-write was able to successfully pattern human dermal fibroblasts with high post-transfer viability (91% ± 3%) and no observed double-strand DNA damage. As seen with atomic force microscopy, gelatin offers a unique benefit in that it is present temporarily to allow cell transfer, but melts and is removed with incubation to reveal the desired application-specific growth surface. This provides unobstructed cellular growth after printing. Monitoring cell location after transfer, we show that melting and removal of gelatin does not affect cellular placement; cells maintained registry within 5.6 ± 2.5 μm to the initial pattern. This study demonstrates the effectiveness of gelatin in laser direct-writing to create spatially precise cell patterns with the potential for applications in tissue engineering, stem cell, and cancer research.
- Published
- 2011
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- View/download PDF
36. The maintenance of pluripotency following laser direct-write of mouse embryonic stem cells.
- Author
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Raof NA, Schiele NR, Xie Y, Chrisey DB, and Corr DT
- Subjects
- Animals, Cell Line, Cell Survival radiation effects, Immunohistochemistry, Mice, Cell Differentiation radiation effects, Embryonic Stem Cells cytology, Embryonic Stem Cells radiation effects, Lasers
- Abstract
The ability to precisely pattern embryonic stem (ES) cells in vitro into predefined arrays/geometries may allow for the recreation of a stem cell niche for better understanding of how cellular microenvironmental factors govern stem cell maintenance and differentiation. In this study, a new gelatin-based laser direct-write (LDW) technique was utilized to deposit mouse ES cells into defined arrays of spots, while maintaining stem cell pluripotency. Results obtained from these studies showed that ES cells were successfully printed into specific patterns and remained viable. Furthermore, ES cells retained the expression of Oct4 in nuclei after LDW, indicating that the laser energy did not affect their maintenance of an undifferentiated state. The differentiation potential of mouse ES cells after LDW was confirmed by their ability to form embryoid bodies (EBs) and to spontaneously become cell lineages representing all three germ layers, revealed by the expression of marker proteins of nestin (ectoderm), Myf-5 (mesoderm) and PDX-1 (endoderm), after 7 days of cultivation. Gelatin-based LDW provides a new avenue for stem cell patterning, with precision and control of the cellular microenvironment., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
37. Metallic foil-assisted laser cell printing.
- Author
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Lin Y, Huang Y, and Chrisey DB
- Subjects
- Cell Count, Cell Survival, HT29 Cells, Humans, Printing instrumentation, Lasers, Metals, Printing methods
- Abstract
Laser direct-write technology such as modified laser-induced forward transfer (LIFT) is emerging as a revolutionary technology for biological construct fabrication. While many modified LIFT-based cell direct writing successes have been achieved, possible process-induced cell injury and death is still a big hurdle for modified LIFT-based cell direct writing to be a viable technology. The objective of this study is to propose metallic foil-assisted LIFT using a four-layer structure to achieve better droplet size control and increase cell viability in direct writing of human colon cancer cells (HT-29). The proposed four layers include a quartz disk, a sacrificial and adhesive layer, a metallic foil, and a cell suspension layer. The bubble formation-induced stress wave is responsible for droplet formation. It is found that the proposed metallic foil-assisted LIFT approach is an effective cell direct-write technology and provides better printing resolution and high post-transfer cell viability when compared with other conventional modified LIFT technologies such as matrix-assisted pulsed-laser evaporation direct-write; at the same time, the possible contamination from the laser energy absorbing material is minimized using a metallic foil.
- Published
- 2011
- Full Text
- View/download PDF
38. Generation of Ag2O micro-/nanostructures by pulsed excimer laser ablation of Ag in aqueous solutions of polysorbate 80.
- Author
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Yan Z, Bao R, and Chrisey DB
- Subjects
- Molecular Structure, Oxides chemistry, Particle Size, Silver Compounds chemistry, Solutions, Surface Properties, Water chemistry, Lasers, Nanostructures chemistry, Oxides chemical synthesis, Polysorbates chemistry, Silver chemistry, Silver Compounds chemical synthesis
- Abstract
A new route to synthesis of Ag(2)O micro-/nanostructures, including a mixture of cubes, pyramids, triangular plates, pentagonal rods, and bars, has been developed by pulsed excimer laser ablation of bulk silver in water using polysorbate 80 as surfactant. The polysorbate 80 played an important role in the formation of the Ag(2)O structures, and similar structures could be obtained in polysorbates 20 and 40 aqueous solutions. We have proposed a mechanism to explain the formation of Ag(2)O structures. This laser ablation method provides a unique approach to discover and fabricate new Ag(2)O morphologies.
- Published
- 2011
- Full Text
- View/download PDF
39. Laser-based direct-write techniques for cell printing.
- Author
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Schiele NR, Corr DT, Huang Y, Raof NA, Xie Y, and Chrisey DB
- Subjects
- Animals, Cell Physiological Phenomena, Humans, Neoplasms pathology, Stem Cells cytology, Biomedical Research methods, Biotechnology methods, Cell Culture Techniques methods, Lasers, Microtechnology methods, Tissue Engineering methods
- Abstract
Fabrication of cellular constructs with spatial control of cell location (+/-5 microm) is essential to the advancement of a wide range of applications including tissue engineering, stem cell and cancer research. Precise cell placement, especially of multiple cell types in co- or multi-cultures and in three dimensions, can enable research possibilities otherwise impossible, such as the cell-by-cell assembly of complex cellular constructs. Laser-based direct writing, a printing technique first utilized in electronics applications, has been adapted to transfer living cells and other biological materials (e.g., enzymes, proteins and bioceramics). Many different cell types have been printed using laser-based direct writing, and this technique offers significant improvements when compared to conventional cell patterning techniques. The predominance of work to date has not been in application of the technique, but rather focused on demonstrating the ability of direct writing to pattern living cells, in a spatially precise manner, while maintaining cellular viability. This paper reviews laser-based additive direct-write techniques for cell printing, and the various cell types successfully laser direct-written that have applications in tissue engineering, stem cell and cancer research are highlighted. A particular focus is paid to process dynamics modeling and process-induced cell injury during laser-based cell direct writing.
- Published
- 2010
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40. Excimer laser ablation of a Pt target in water: the observation of hollow particles.
- Author
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Yan Z, Bao R, and Chrisey DB
- Abstract
Micro/nanoparticles were fabricated by pulsed-excimer-laser ablation of a Pt target in water. Three kinds of hollow Pt particles (coalesced by micrograins, assembled by nanocrystals or with smooth shells) were observed together with solid particles using different laser fluences (2.3-6.8 J cm(-2)) and after 6000 laser shots. We propose that the hollow particles were formed on laser-produced bubbles which provided thermodynamically preferred nucleation sites and diffusion sinks for the laser-fabricated Pt clusters or particles. Although the hollow particles are a small proportion, the results have extended the scope of particles that pulsed-laser ablation in liquid can fabricate, and have enriched the mechanistic scenario of laser ablation and nanostructure formation in liquid.
- Published
- 2010
- Full Text
- View/download PDF
41. Process-Induced Cell Injury in Laser Direct Writing of Human Colon Cancer Cells.
- Author
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Lin Y, Huang G, Huang Y, Tzeng TR, and Chrisey DB
- Abstract
Matrix-assisted pulsed-laser evaporation direct-write has emerged as a promising technique for biological construct fabrication. The posttransfer cell viability in matrix-assisted pulsed-laser evaporation direct-write depends on various operating conditions such as the applied laser fluence. To date, the effects of operating conditions such as laser fluence, direct-writing height, and cell density on the posttransfer cell viability have not been well elucidated. This study investigates the effects of operating conditions on the posttransfer cell viability in laser direct writing of human colon cancer HT-29 cells. It has been observed that (1) the HT-29 cell viability decreases from 95% to 78% as the laser fluence increases from 258 to 1482 mJ/cm(2), and the posttransfer cell proliferation capacity does not vary significantly as the laser fluence changes; (2) the direct-writing height does not have noticeable effect on the posttransfer cell viability under low laser fluences (258 and 869 mJ/cm(2)). However, a larger height (such as 29.3 mm) led to an almost 8% viability improvement compared with that of 16.6 mm under a high laser fluence (1482 mJ/cm(2)); and (3) the posttransfer cell viability is not dependent on the cell density for a range from 1 × 10(6) to 1 × 10(7) cells/mL.
- Published
- 2010
- Full Text
- View/download PDF
42. Cellular motors for molecular manufacturing.
- Author
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Dinu CZ, Chrisey DB, Diez S, and Howard J
- Subjects
- Biological Transport physiology, Kinesins physiology, Microtubules physiology
- Abstract
Cells are composed of macromolecular structures of various sizes that act individually or collectively to maintain their viability and perform their function within the organism. This review focuses on one structure, the microtubule, and one of the motor proteins that move along it, conventional kinesin (kinesin 1). Recent work on the cellular functions of kinesins, such as the organization of microtubules during cellular division and the movement of the organelles and vesicles, offers insights into how biological motors might prove useful for organizing structures in engineered environments., ((c) 2007 Wiley-Liss, Inc.)
- Published
- 2007
- Full Text
- View/download PDF
43. Laser microfabrication of hydroxyapatite-osteoblast-like cell composites.
- Author
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Doraiswamy A, Narayan RJ, Harris ML, Qadri SB, Modi R, and Chrisey DB
- Subjects
- Cell Line, Tumor, Cell Proliferation, Cell Survival, Composite Resins, Extracellular Matrix, Humans, Zirconium, Hydroxyapatites therapeutic use, Lasers, Osteoblasts cytology, Osteogenesis, Tissue Engineering methods
- Abstract
We have developed a novel approach for layer-by-layer growth of tissue-engineered materials using a direct writing process known as matrix assisted pulsed laser evaporation direct write (MAPLE DW). Unlike conventional cell-seeding methods, this technique provides the possibility for cell-material integration prior to artificial tissue fabrication. This process also provides greater flexibility in selection and processing of scaffold materials. In addition, MAPLE DW offers rapid computer-controlled deposition of mesoscopic voxels at high spatial resolutions. We have examined MAPLE DW processing of zirconia and hydroxyapatite scaffold materials that can provide a medical device with nearly inert and bioactive implant-tissue interfaces, respectively. We have also demonstrated codeposition of hydroxyapatite, MG 63 osteoblast-like cells, and extracellular matrix using MAPLE DW. We have shown that osteoblast-like cells remain viable and retain the capacity for proliferation when codeposited with bioceramic scaffold materials. Our results on MG 63-hydroxyapatite composites can be extended to develop other integrated cell-scaffold structures for medical and dental applications.
- Published
- 2007
- Full Text
- View/download PDF
44. Three-dimensional direct writing of B35 neuronal cells.
- Author
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Patz TM, Doraiswamy A, Narayan RJ, He W, Zhong Y, Bellamkonda R, Modi R, and Chrisey DB
- Subjects
- Animals, Biocompatible Materials, Cell Culture Techniques, Cell Line, Tumor, Drug Combinations, Rats, Collagen, Laminin, Neurons, Proteoglycans, Tissue Engineering
- Abstract
We have demonstrated two-dimensional and three-dimensional transfer of B35 neuronal cells onto and within polymerized Matrigel substrates, using matrix-assisted pulsed laser evaporation-direct write (MDW). The B35 cells were transferred from a quartz ribbon to depths of up to 75 microm by systematically varying the fluence emitted from the ArF (lambda = 193 nm) laser source. MDW-transferred cells were examined using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL), 4',6-diamidino-2-phenylindole (DAPI), and alpha-tubulin staining. Confocal microscopy has shown that the transferred B35 cells extended their axons outward in three dimensions within the polymerized Matrigel substrate. The B35 cells made axonal connections and formed a three-dimensional neural network within 72 h after MDW transfer. In addition, TUNEL staining demonstrated that only 3% of the B35 cells underwent apoptosis after being transferred using the MDW process. MDW and other emergent direct write processes may provide unique approaches for creating layered, heterogeneous, three-dimensional cell-seeded scaffolds for use in peripheral nerve repair.
- Published
- 2006
- Full Text
- View/download PDF
45. Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices.
- Author
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Doraiswamy A, Jin C, Narayan RJ, Mageswaran P, Mente P, Modi R, Auyeung R, Chrisey DB, Ovsianikov A, and Chichkov B
- Subjects
- Animals, Drug Carriers, Materials Testing methods, Microchemistry methods, Microscopy, Electron, Scanning, Neurons cytology, Neurons ultrastructure, X-Ray Diffraction, Equipment Design methods, Equipment and Supplies, Inorganic Chemicals chemistry, Organic Chemicals chemistry, Photons
- Abstract
Three-dimensional microstructured medical devices, including microneedles and tissue engineering scaffolds, were fabricated by two photon induced polymerization of Ormocer organic-inorganic hybrid materials. Femtosecond laser pulses from a titanium:sapphire laser were used to break chemical bonds on Irgacure 369 photoinitiator within a small focal volume. The radicalized starter molecules reacted with Ormocer US-S4 monomers to create radicalized polymolecules. The desired structures are fabricated by moving the laser focus in three dimensions using a galvano-scanner and a micropositioning system. Ormocer surfaces fabricated using two photon induced polymerization demonstrated acceptable cell viability and cell growth profiles against B35 neuroblast-like cells and HT1080 epithelial-like cells. Lego-like interlocking tissue engineering scaffolds and microneedle arrays with unique geometries were created using two photon induced polymerization. These results suggest that two photon induced polymerization is able to create medical microdevices with a larger range of sizes, shapes, and materials than chemical isotropic etching, injection molding, reactive ion etching, surface micromachining, bulk micromachining, polysilicon micromolding, lithography-electroforming-replication, or other conventional microfabrication techniques.
- Published
- 2006
- Full Text
- View/download PDF
46. Survival and proliferative ability of various living cell types after laser-induced forward transfer.
- Author
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Hopp B, Smausz T, Kresz N, Barna N, Bor Z, Kolozsvári L, Chrisey DB, Szabó A, and Nógrádi A
- Subjects
- Animals, Astrocytes cytology, Cell Culture Techniques, Cell Survival physiology, Cells, Cultured, Epithelial Cells cytology, Lasers, Lens, Crystalline cytology, Rats, Schwann Cells cytology, Swine, Tissue Engineering methods, Astrocytes physiology, Cell Differentiation physiology, Cell Proliferation, Epithelial Cells physiology, Lens, Crystalline physiology, Schwann Cells physiology
- Abstract
The survival, proliferation, and differentiation of freshly isolated and cultured cells were studied after absorbing film-assisted laser-induced forward transfer. Rat Schwann and astroglial cells and pig lens epithelial cells were used for transfer and the cells were cultured for 2 weeks after laser-pulsed transfer. All three cell types survived, proliferated, and differentiated under cell culture conditions and regained their original phenotype a few days after cell transfer. Time resolution studies have shown that the time required to accelerate the jets and droplets containing the cells was less than 1 micros and that the estimated minimum average acceleration of those ejected cells that reached a constant velocity was approximately 10(7) x g. This suggests that the majority of studied cells tolerated the extremely high acceleration at the beginning of the ejection and the deceleration during impact on the acceptor plate without significant damage to the original phenotype. These results suggest that the absorbing film-assisted laser-induced forward transfer technique appears to be suitable for several potential applications in tissue engineering and the biomedical tissue repair technologies.
- Published
- 2005
- Full Text
- View/download PDF
47. Laser printing of pluripotent embryonal carcinoma cells.
- Author
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Ringeisen BR, Kim H, Barron JA, Krizman DB, Chrisey DB, Jackman S, Auyeung RY, and Spargo BJ
- Subjects
- Animals, Cell Survival physiology, DNA Damage physiology, Immunohistochemistry, Mice, Microscopy, Fluorescence, Tumor Cells, Cultured, Carcinoma, Embryonal metabolism, Cell Differentiation physiology
- Abstract
A technique by which to print patterns and multilayers of scaffolding and living cells could be used in tissue engineering to fabricate tissue constructs with cells, materials, and chemical diversity at the micron scale. We describe here studies using a laser forward transfer technology to print single-layer patterns of pluripotent murine embryonal carcinoma cells. This report focuses on verifying cell viability and functionality as well as the ability to differentiate cells after laser transfer. We find that when cells are printed onto model tissue scaffolding such as a layer of hydrogel, greater than 95% of the cells survive the transfer process and remain viable. In addition, alkaline comet assays were performed on transferred cells, showing minimal single-strand DNA damage from potential ultraviolet-cell interaction. We also find that laser-transferred cells express microtubular associated protein 2 after retinoic acid stimulus and myosin heavy chain protein after dimethyl sulfoxide stimulus, indicating successful neural and muscular pathway differentiation. These studies provide a foundation so that laser printing may next be used to build heterogeneous multilayer cellular structures, enabling cell growth and differentiation in heterogeneous three-dimensional environments to be uniquely studied.
- Published
- 2004
- Full Text
- View/download PDF
48. Laser deposition of polymer and biomaterial films.
- Author
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Chrisey DB, Piqué A, McGill RA, Horwitz JS, Ringeisen BR, Bubb DM, and Wu PK
- Subjects
- Composite Resins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Biocompatible Materials chemistry, Polymers chemistry
- Published
- 2003
- Full Text
- View/download PDF
49. Picoliter-scale protein microarrays by laser direct write.
- Author
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Ringeisen BR, Wu PK, Kim H, Piqué A, Auyeung RY, Young HD, Chrisey DB, and Krizman DB
- Subjects
- Equipment Design, Feasibility Studies, Lasers, Microchemistry instrumentation, Microchemistry methods, Miniaturization, Quality Control, Nanotechnology instrumentation, Nanotechnology methods, Protein Array Analysis instrumentation, Protein Array Analysis methods
- Abstract
We demonstrate the accurate picoliter-scale dispensing of active proteins using a novel laser transfer technique. Droplets of protein solution are dispensed onto functionalized glass slides and into plastic microwells, activating as small as 50-microm diameter areas on these surfaces. Protein microarrays fabricated by laser transfer were assayed using standard fluorescent labeling techniques to demonstrate successful protein and antigen binding. These results indicate that laser transfer does not damage the active site of the dispensed protein and that this technique can be used to successfully fabricate a functioning protein microarray. Also, as a result of the efficient nature of the process, material usage is reduced by two to four orders of magnitude compared to conventional pin dispensing methods for protein spotting.
- Published
- 2002
- Full Text
- View/download PDF
50. Generation of mesoscopic patterns of viable Escherichia coli by ambient laser transfer.
- Author
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Ringeisen BR, Chrisey DB, Piqué A, Young HD, Jones-Meehan J, Modi R, Bucaro M, and Spargo BJ
- Subjects
- Green Fluorescent Proteins, Lasers, Luminescent Proteins genetics, Microscopy, Electron, Escherichia coli cytology, Escherichia coli ultrastructure
- Abstract
We have generated mesoscopic patterns of viable Escherichia coli on Si(1 1 1), glass, and nutrient agar plates by using a novel laser-based transfer process termed matrix assisted pulsed laser evaporation direct write (MAPLE DW). We observe no alterations to the E. coli induced by the laser-material interaction or the shear forces during the transfer. Transferred E. coli patterns were observed by optical and electron microscopes, and cell viability was shown through green fluorescent protein (GFP) expression and cell culturing experiments. The transfer mechanism for our approach appears remarkably gentle and suggests that active biomaterials such as proteins, DNA and antibodies could be serially deposited adjacent to viable cells. Furthermore, this technique is a direct write technology and therefore does not involve the use of masks, etching, or other lithographic tools.
- Published
- 2002
- Full Text
- View/download PDF
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