1. Cytotoxicity of polymers intended for the extrusion-based additive manufacturing of surgical guides
- Author
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Felix Burkhardt, Benedikt C. Spies, Christian Wesemann, Carl G. Schirmeister, Erik H. Licht, Florian Beuer, Thorsten Steinberg, and Stefano Pieralli
- Subjects
Medicine ,Science - Abstract
Abstract Extrusion-based printing enables simplified and economic manufacturing of surgical guides for oral implant placement. Therefore, the cytotoxicity of a biocopolyester (BE) and a polypropylene (PP), intended for the fused filament fabrication of surgical guides was evaluated. For comparison, a medically certified resin based on methacrylic esters (ME) was printed by stereolithography (n = 18 each group). Human gingival keratinocytes (HGK) were exposed to eluates of the tested materials and an impedance measurement and a tetrazolium assay (MTT) were performed. Modulations in gene expression were analyzed by quantitative PCR. One-way ANOVA with post-hoc Tukey tests were applied. None of the materials exceeded the threshold for cytotoxicity (
- Published
- 2022
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