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Objective: This study was to evaluate the interaction of three different light-emitting diode (LED) light colors (white, green, and blue) and three intensities (5, 10, and 15 lx) on slaughter performance, meat quality and serum antioxidant capacity of broilers raised in three-layer cages.Methods: A total of 648 (8-days-old) male broiler chicks (Cobb-500) were randomly assigned in 3×3 factorially arranged treatments: three light colors (specifically, white, blue, and green) and three light intensities (namely, 5, 10, and 15 lx) for 35 days. Each treatment consisted of 6 replicates of 12 chicks. The test lasted for 35 days.Results: The semi-eviscerated weight percentage (SEWP) in 5 lx white was higher than that in 15 lx (p

// Chaoxiong Zhang 1, 2, * , Ning Wu 3, * , Fu Gao 1, * , Jiaqi Han 1 , Yanyong Yang 1 , Chuanfeng Zhou 1 , Weimin Sun 4 , Linfeng Xian 1 , Ying Cheng 1 , Bailong Li 1 , Jianming Cai 1 , Cong Liu 1 1 Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, PR China 2 Institute of Military Medicine, Chengdu Military Region, Chengdu, 610021, China 3 Department of Respiratory Medicine, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China 4 Department of Immunology, Second Military Medical University, Shanghai, 200433, People’s Republic of China * These authors contributed equally to this work Correspondence to: Jianming Cai, email: caijianming882003@163.com Cong Liu, email: victorliu20102020@163.com Keywords: MTERFD1, cancers, prognosis, proliferation, survival Received: October 02, 2015 Accepted: June 16, 2016 Published: August 09, 2016 ABSTRACT MTERFD1, also named MTERF3 (mitochondrial transcription termination factor 3), regulates transcription of the mitochondrial genome. MTERFD1 is a mitochondrial protein that represses mammalian mitochondrial DNA initiation in vivo . In this study, we found that MTERFD1 gene amplification and high expression existed in many different types of cancer. Significantly, increased expression of MTERFD1 gene was correlated with lower overall survival rate in clinical. Overexpression of MTERFD1 gene promoted to tumor cell growth in vivo and in vitro and increased the percentage of cells in S phase. In conclusion, our data firstly indicated the MTERFD1 was an oncogene in many types of cancer.

Recent studies suggest that mangiferin aglycone (norathyriol) has great potential as a novel radioprotector without any known toxic side effects. In this study, we assessed the protective effects of mangiferin aglycone against radiation-induced injuries on normal human intestinal epithelial cells (HIECs), while using mangiferin as a reference compound. The in vitro experiments showed that pretreatment of either mangiferin aglycone or mangiferin could inhibit cytotoxic effects of ionizing irradiation (IR) on HIECs. Cellular changes were estimated by measuring cell viability, clonogenic surviving rate, and apoptotic rate. Compared to mangiferin, we found mangiferin aglycone had greater radioprotective effects of mangiferin aglycone on HIECs. It has been demonstrated that the cytotoxicity of ionizing radiation relates to its capacity to induce DNA damage. In view of this, we monitored DNA double-strand breaks (DSBs) using γH2AX foci formation to test whether mangiferin aglycone and mangiferin could modulate genotoxic effects of radiation. It shows that mangiferin aglycone could eliminate 46.8% of the total DSBs of the cells exposed to 2 Gy IR, which is significantly better than mangiferin. Complementing earlier results from our group, it appears possible to conclude that mangiferin aglycone presents potential useful effects on IR-induced damage and may be a better radioprotective agent than mangiferin therapeutically.

Bone marrow is a major site of radiation injury. The extreme sensitivity of bone marrow cells to genotoxic stress largely determines the adverse side effects of radiation. CpG-oligodeoxynucleotide (ODN) is known to be radioprotective in extramedullary hemopoiesis, but its effect on bone marrow hemopoiesis remains unknown. In this study, we investigated whether CpG-ODN ameliorated hemopoiesis radiation injury when administered after total-body irradiation (TBI). Mice were treated with 50 μg of CpG-ODN via intraperitoneal injection (i.p) 30 min., 24 and 48 hr after TBI. Our results show that CpG-ODN was able to mediate the activation of nuclear factor κB (NF-κB) via degradation of inhibitor NF-κB (IκB-α), and some oxidative stress parameters (malondialdehyde, glutathione and superoxide dismutase) showed significant differences between the radiation control group and the radiation and administration of CpG-ODN group. White blood cell count, bone marrow cell count and bone marrow histological examination indicated that CpG-ODN minimized bone marrow damage induced by radiation. Exogenous colony-forming unit-spleen count indicated that CpG-ODN reduced primitive hemopoietic stem cell damage and reconstituted the hemopoietic system after TBI. The survival of mice was also enhanced after various levels of TBI. The calculated dose reduction factor was 1.2. Thus, we conclude that CpG-ODN may contribute to the amelioration of hemopoiesis radiation injury.

Ionizing radiation (IR) is a well-known carcinogen, however the mechanism of radiation induced thymic lymphoma is not well known. Moreover, an easy and effective method to protect mice from radiation induced thymic lymphoma is still unknown. Hydrogen, or H(2), is seldom regarded as an important agent in medical usage, especially as a therapeutic gas. Here in this study, we found that H(2) protects mice from radiation induced thymic lymphoma in BALB/c mice.

Sina Weibo, as the most popular micro-blog platform in China, has become a major source of network hot events and sensitive public opinion. This paper presents a scheme to predict the repost number of micro-blog message. Curve fitting and time-series model are used for the prediction. In order to improve the predicting precision, an empirical correction model are built by utilizing the prediction data of 3200 micro-blog messages using least square and second-order polynomial regression methods, which takes the daily periodic fluctuation of reposting probability into consideration. By experimental verification, the proposed scheme can predict the repost number of micro-blog message accurately.

The role of Toll-like receptor-2 (TLR2) in radio-resistance remained largely unknown. TLR2 knockout (TLR2−/−) mice received radiation of 6.5 Gy and then were studied. We found that radiation resulted in more severe mortality and morbidity rates in TLR2−/− mice. The cause of death in TLR2−/− mice may be severe and persistent bone marrow cell loss. Injection of the TLR2 agonist Pam3CSK4 into wild type (WT) mice induced radio-resistance. Myd88−/− mice were more susceptible to radiation. In conclusion, our data indicate that, similar to TLR4, TLR2 plays a critical role in radio-resistance.

To effectively analyze micro blogging public opinion information, micro blog data acquisition is a vital first step. For directional Weibo users' data acquisition, traditional Application Programming Interface (API) and directional Web crawler are conditional. This paper focuses on the opinion leaders on Sina Weibo, who are divided into several categories in different fields by their label characteristics and previous published contents. A method of micro blog data acquisition based on follow-group mode is presented in this paper by making full use of Weibo working features. It can receive opinion leaders' micro blog message list, which system pushes automatically. By experimental analysis, the method of follow-group is practical and feasible. Compared with traditional methods, it can avoid the possible suspension of Sina Weibo account and get micro blog important data without missing. Moreover, it can guarantee the integrity and real-time performance of micro blog data, thus providing a comprehensive and accurate data support for analysis of public opinion.

Sina Weibo, as one of the most popular and fast growing social network, has gradually become the field where hot topics appear, propagate, and outbreak. In order to discriminate and find out hot topics in micro-blog information, we conduct a series of studies on Sina Weibo, and one of our key findings is that opinion leaders play a very important role in the propagation of hot topics. A smart discriminant model is proposed in this paper to detect hot topics in time, which takes the structure information and propagation characteristics of Sina Weibo as well as the users' influence into consideration. Moreover, word co-occurrence graph is used to extract and display topics. This model has some excellent characters such as a low coupling degree between modules and a low requirement for the amount of data. By experimental verification, it can detect hot topics effectively. Index Terms - Sina Weibo, hot topic, discriminant model, opinion leader, word co-occurrence graph

In the recent few years, with swift development of informatization technology, as a newly born talent recruitment mode, internet recruitment has gradually received wide recognition and favor in China and has become one of the major channels for enterprise recruitment and personal job hunting. For enhance effectiveness of internet recruitment, we should enhance vocational quality of network recruitment personnel, improve relevant laws and regulations, set up effective management system, and bring in moral education.

Summary Background Radiation often causes depletion of immunocytes in tissues and blood, which results in immunosuppression. Molecular hydrogen (H2) has been shown in recent studies to have potential as a safe and effective radioprotective agent through scavenging free radicals. This study was designed to test the hypothesis that H2 could protect immunocytes from ionizing radiation (IR). Material/Methods H2 was dissolved in physiological saline or medium using an apparatus produced by our department. A 2-[6-(4′-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) probe was used to detect intracellular hydroxyl radicals (•OH). Cell apoptosis was evaluated by annexin V-FITC and Propidium iodide (PI) staining as well as the caspase 3 activity. Finally, we examined the hematological changes using an automatic Sysmex XE 2100 hematology analyzer. Results We demonstrated H2-rich medium pretreatment reduced •OH level in AHH-1 cells. We also showed H2 reduced radiation-induced apoptosis in thymocytes and splenocytes in living mice. Radiation-induced caspase 3 activation was also attenuated by H2 treatment. Finally, we found that H2 rescued the radiation-caused depletion of white blood cells (WBC) and platelets (PLT). Conclusions This study suggests that H2 protected the immune system and alleviated the hematological injury induced by IR.

Bone marrow is a major site of radiation injury. The extreme sensitivity of bone marrow cells to genotoxic stress largely determines the adverse side effects of radiation. CpG-oligodeoxynucleotide (ODN) is known to be radioprotective in extramedullary hemopoiesis, but its effect on bone marrow hemopoiesis remains unknown. In this study, we investigated whether CpG-ODN ameliorated hemopoiesis radiation injury when administered after total-body irradiation (TBI). Mice were treated with 50 μg of CpG-ODN via intraperitoneal injection (i.p) 30 min., 24 and 48 hr after TBI. Our results show that CpG-ODN was able to mediate the activation of nuclear factor κB (NF-κB) via degradation of inhibitor NF-κB (IκB-α), and some oxidative stress parameters (malondialdehyde, glutathione and superoxide dismutase) showed significant differences between the radiation control group and the radiation and administration of CpG-ODN group. White blood cell count, bone marrow cell count and bone marrow histological examination indicated that CpG-ODN minimized bone marrow damage induced by radiation. Exogenous colony-forming unit-spleen count indicated that CpG-ODN reduced primitive hemopoietic stem cell damage and reconstituted the hemopoietic system after TBI. The survival of mice was also enhanced after various levels of TBI. The calculated dose reduction factor was 1.2. Thus, we conclude that CpG-ODN may contribute to the amelioration of hemopoiesis radiation injury.