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Brain radiomics can reflect the characteristics of brain pathophysiology. However, the value of T1-weighted images, quantitative susceptibility mapping, and R2* mapping in the diagnosis of Parkinson's disease (PD) was underestimated in previous studies. In this prospective study to establish a model for PD diagnosis based on brain imaging information, we collected high-resolution T1-weighted images, R2* mapping, and quantitative susceptibility imaging data from 171 patients with PD and 179 healthy controls recruited from August 2014 to August 2019. According to the inclusion time, 123 PD patients and 121 healthy controls were assigned to train the diagnostic model, while the remaining 106 subjects were assigned to the external validation dataset. We extracted 1408 radiomics features, and then used data-driven feature selection to identify informative features that were significant for discriminating patients with PD from normal controls on the training dataset. The informative features so identified were then used to construct a diagnostic model for PD. The constructed model contained 36 informative radiomics features, mainly representing abnormal subcortical iron distribution (especially in the substantia nigra), structural disorganization (e.g., in the inferior temporal, paracentral, precuneus, insula, and precentral gyri), and texture misalignment in the subcortical nuclei (e.g., caudate, globus pallidus, and thalamus). The predictive accuracy of the established model was 81.1 ± 8.0% in the training dataset. On the external validation dataset, the established model showed predictive accuracy of 78.5 ± 2.1%. In the tests of identifying early and drug-naïve PD patients from healthy controls, the accuracies of the model constructed on the same 36 informative features were 80.3 ± 7.1% and 79.1 ± 6.5%, respectively, while the accuracies were 80.4 ± 6.3% and 82.9 ± 5.8% for diagnosing middle-to-late PD and those receiving drug management, respectively. The accuracies for predicting tremor-dominant and non-tremor-dominant PD were 79.8 ± 6.9% and 79.1 ± 6.5%, respectively. In conclusion, the multiple-tissue-specific brain radiomics model constructed from magnetic resonance imaging has the ability to discriminate PD and exhibits the advantages for improving PD diagnosis.

Silica ceramic cores have played an important part in the manufacture of hollow blades due to their excellent chemical stability and moderate high-temperature mechanical properties. In this study, silica-based ceramics were prepared with Al2O3 addition by stereolithography, and the influence of Al2O3 content on mechanical properties of the silica-based ceramics was investigated. The Al2O3 in silica-based ceramics can improve the mechanical properties by playing a role as a seed for the crystallization of fused silica into cristobalite. As a result, with the increase of Al2O3 content, the linear shrinkage of the silica-based ceramics first decreased and then increased, while the room-temperature flexural strength and the high-temperature flexural strength first increased and then decreased. As the Al2O3 content increased to 1.0 vol%, the linear shrinkage was reduced to 1.64% because of the blocked viscous flow caused by Al2O3. Meanwhile, the room-temperature flexural strength and the high-temperature flexural strength were improved to 20.38 and 21.43 MPa with 1.0 vol% Al2O3, respectively, due to the increased α-cristobalite and β-cristobalite content. Therefore, using the optimal content of Al2O3 in silica-based ceramics can provide excellent mechanical properties, which are suitable for the application of ceramic cores in the manufacturing of hollow blades.

Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in practice. The immobilized laccase on the polyethylenimine (PEI)‐functionalized magnetic nanoparticles (Fe3O4–NH2–PEI–laccase) was fabricated to efficiently degrade phenolic compounds continuously in a newly fixed bed reactor under a high‐gradient magnetic field. The degradation rate of continuous treatment in the bed after 18 h was 2.38 times as high as that of batch treatment after six successive operations with the same treatment duration. Under the optimal conditions of volume fraction of nickel wires mesh, flow rate of phenol solution, phenol concentration, and Fe3O4–NH2–PEI–laccase amount, the degradation rate of phenol kept over 70.30% in 48 h continuous treatment. The fixed bed reactor filled with Fe3O4–NH2–PEI–laccase provided a promising avenue for the continuous biodegradation of phenolic compounds for industrial wastewater in practice.

Keloid resection followed by adjuvant radiotherapy is the most efficacious treatment for keloids. However, for earlobe keloids, an optimal protocol for the total dose and fractions of adjuvant radiation has not yet been established. We retrospectively analyzed the efficacy and safety of immediate three-fraction electron radiotherapy after operation for resistant earlobe keloids. From 2011 to 2017, three-fraction electron radiotherapy with single dose of 5 Gy was given postoperatively to 23 patients with 30 keloids in our hospital. The first fraction of adjuvant radiotherapy was administered within 2 h of surgery, and the other two sessions were completed within the next day or two. Five (16.7%) primary keloids and 25 (83.3%) recurrent keloids were examined in this study. The primary endpoint was the local control rate, which was 86.7% after a median follow-up of 26 months (14–93 months). Secondary endpoints were acute and late procedure-related complications, and no severe complications were observed after combination therapy. Our results suggest that three-fraction electron radiotherapy after excision within 2 days of surgery is a safe and effective protocol for the prevention of earlobe keloid recurrence that can also improve patient compliance and comfort.

Silica ceramic cores have played an important role in the manufacture of hollow blades due to its excellent chemical stability and moderate high-temperature mechanical properties. In this study, silica-based ceramics were prepared by stereolithography and the influence of Al2O3 content on mechanical properties of the silica-based ceramics was investigated. With the increase of Al2O3 content, the linear shrinkage of the silica-based ceramics first decreased and then increased, while the room-temperature flexural strength and the high-temperature flexural strength first increased and then decreased. As the Al2O3 content increased to 1.0 vol.%, the linear shrinkage was reduced to 1.64% because of the blocked viscous flow. Meanwhile, the room-temperature flexural strength and the high-temperature flexural strength were respectively improved to 20.38 MPa and 21.43 MPa with 1.0 vol.% Al2O3 due to the increased α-cristobalite and β-cristobalite content. Therefore, using the optimal content of Al2O3 in silica-based ceramics can provide excellent mechanical properties and ensure the reliability of silica-based ceramic cores for manufacturing hollow blades.

To investigate the therapeutic effect of scalp acupuncture and rehabilitation training on balance dysfunction in children with spasmodic hemiplegia so as to provide the reference to the optimization of treatment scheme.A total of 60 children with spastic hemiplegia were divided into a routine group and a scalp acupuncture group, 30 cases in each one according to random number table. In the routine group, the rehabilitation training was provided, including exercise training, balance training, spasmotherapy apparatus, electromyography biofeedback apparatus and orthoses. In the scalp acupuncture group, on the base of the treatment as the routine group, scalp acupuncture was supplemented at motor area, foot motor sensory area, equilibrium area and parietal temporal anterior oblique line. Separately, before the treatment, after 3 months treatment and after 6 months treatment, the dimension D and E of the gross motor function measure-88 (GMFM-88) and Berg balance scale (BBS) were adopted to evaluate balance related motor functions and equilibrium function. The differences in the above 3 indicators at different time stages were compared in children between the two groups.Compared with the score before the treatment, BBS score was obviously increased after 3 and 6 months treatment in the patients of the two groups respectively (On the base of routine rehabilitation training, scalp acupuncture can improve balance function of children with spastic hemiplegia better.

To investigate the effect of Baixiao moxibustion at meridian sinew nodal points combined with routine rehabilitation on upper limb motor function in children with spastic hemiplegic cerebral palsy.A total of 50 children with spastic hemiplegic cerebral palsy were divided into control group and treatment group using a random number table, with 25 children in each group. The children in the control group were given routine rehabilitation training of the ipsilateral upper and lower limbs, and those in the treatment group were given Baixiao moxibustion at the meridian sinew nodal points of the ipsilateral upper limb in addition to the treatment in the control group, once a day and five times a week. Each course of treatment was 4 consecutive weeks, and both groups were treated for 3 courses. Before treatment and at weeks 4 and 12 of treatment, modified Ashworth score was used to evaluate muscle tension of the ipsilateral upper limb, and 88-item version of the Gross Motor Function Measure (GMFM-88) and Carroll upper extremities functional test (UEFT) were used to assess the motor function of the ipsilateral upper limb.At weeks 4 and 12 of treatment, both groups had a significant reduction in modified Ashworth score (Baixiao moxibustion at meridian sinew nodal points can significantly improve the muscle tension and motor function of the ipsilateral upper limb in children with spastic hemiplegic cerebral palsy, and the improvement becomes more apparent as the treatment lasts longer.

Purpose To investigate how necroptosis (ie, programmed necrosis) is involved in killing of non-small cell lung cancer (NSCLC) after ablative hypofractionated radiation therapy (HFRT). Methods and Materials Deoxyribonucleic acid damage, DNA repair, and the death form of NSCLC cells were assessed after radiation therapy. The overexpression and silencing of receptor-interacting protein kinases 3 (RIP3, a key protein involved activation of necroptosis)-stable NSCLC cell lines were successfully constructed. The form of cell death, the number and area of colonies, and the regulatory proteins of necroptosis were characterized after radiation therapy in vitro. Finally, NSCLC xenografts and patient specimens were used to examine involvement of necroptosis after ablative HFRT in vivo. Results Radiation therapy induced expected DNA damage and repair of NSCLC cell lines, but ablative HFRT at ≥10 Gy per fraction preferentially stimulated necroptosis in NSCLC cells and xenografts with high RIP3 expression, as characterized by induction and activation of RIP3 and mixed-lineage kinase domain-like protein and release of immune-activating chemokine high-mobility group box 1. In contrast, RNA interference of RIP3 attenuated ablative HFRT-induced necroptosis and activation of its regulatory proteins. Among central early-stage NSCLC patients receiving stereotactic body radiation therapy, high expression of RIP3 was associated with improved local control and progression-free survival (all P Conclusions Ablative HFRT at ≥10 Gy per fraction enhances killing of NSCLC with high RIP3 expression via preferential stimulation of necroptosis. RIP3 may serve as a useful biomarker to predict favorable response to stereotactic body radiation therapy.

As illegal access control, privacy disclosure and other threats in vehicular ad-hoc networks become more prevalent, and the importance of safety and privacy protection in vehicular communications grows significantly. The current communication protocols in vehicular communications primarily guarantee infrangibility and unconditional anonymity to achieve security. On board units in vehicular ad-hoc networks form a shared loop called ring signature. In this paper, we propose a dual-protected ring signature algorithm to protect both sending and receiving of a message with secure transmission. We also introduce road side unit-assisted looping to increase ringing probability. The performance of these algorithms is analyzed theoretically and compared with the standard ring signature algorithm in a real vehicle environment. The analysis and comparison results show that the dual-protected ring signature algorithm is superior to the standard ring signature in security, ringing probability, and communication efficiency, and it satisfactorily meets the requirements of secure communication and privacy protection.

Objective: To study the correlation of tumor suppressor gene TAp73 and autophagy gene Beclin1 expression in osteosarcoma tissue with tumor malignancy. Methods: 56 cases of osteosarcoma tissues and peri-osteosarcoma tissues as well as 37 cases of giant cell tumor tissues surgically removed in our hospital between June 2013 and August 2016 were collected, immunohistochemical kits were used to detect the positive expression rate of TAp73 and Beclin1, and enzyme-linked immunosorbent assay kits were used to determine the protein expression TAp73, Beclin1 as well as proliferation and invasion genes. Results: Protein expression and positive expression rate of TAp73 and Beclin1 in osteosarcoma tissues were significantly lower than those in peri-osteosarcoma tissues and giant cell tumor tissues (P

With the constraint that all six major pollutants in Nanjing must meet the air quality standards by 2030, on the basis of the 2015 emission inventory, the CMAQ air quality model was used to conduct PM

T-2 toxin is one of the most toxic trichothecenes and harmful to human health and animal husbandry. The mechanism underlying its growth suppression remains unclear, especially for mitochondrial damage in human gastric epithelial cells. In the present study, we investigated cell death caused by T-2 toxin in a human gastric epithelial cell line (GES-1) and the possible mechanism of T-2-induced cytotoxicity. T-2 strongly reduced the viability of GES-1 cells in a time- and dose-dependent manner within a small range of concentrations. However, when the concentrations of T-2 were >40 nM, there was no concentration dependence, only time dependence. Moreover, T-2 induced apoptosis, with the activation of caspase-3 in GES-1 and mitochondrial membrane potential (MMP) decrease and cytochrome c release. T-2 also resulted in the accumulation of reactive oxygen species (ROS) and DNA damage with a positive signal of p-H2A.X in GES-1 cells. While T-2 caused a MMP decrease, DNA damage and cell death were not blocked by pretreatment with 3 mM glutathione (GSH), a typical scavenger of ROS. The induction of mitochondrial permeability transition pore (mPTP) regulators voltage-dependent anion channel (VDAC1) and cyclophilin D (CypD) were also observed in T-2-treated cells. Interestingly, cyclosporine A (CsA), a CypD inhibitor, significantly reversed the drop in MMP and the DNA damage, as well as ROS accumulation caused by T-2. Additionally, GES-1 cell death could also be protected to some extent by 4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid (DIDS), an inhibitor of VDAC1, especially the combination of CsA and DIDS, and 3 mM GSH could further enhance the effect of CsA + DIDS on cell viability. In conclusion, our present findings indicate that the T-2 induced MMP decrease, DNA damage and cell death, as well as ROS accumulation in GES-1 cells, starts with T-2 directly perturbing the mitochondria triggering ROS generation by acting on CypD and VDAC1. This study presents a new viewpoint for evaluating the toxicity of T-2 toxin.

Coproduction of H2 and volatile fatty acids from sweet sorghum stalks was successfully developed via two-step fermentation process involving alkaline and enzymatic treatment of the residual slurry obtained from the first step fermentation of the raw material. The optimum treatment conditions to obtain the highest yield of the end products included 2% (w/v) alkali at 120 °C for the residual slurry and 32 FPU cellulase per gram of the alkali-treated materials. The two-step fermentation using Clostridium thermosaccharolyticum increased the end product yields of H2 (6.37 mmol/g-substrate) by 95%, acetic acid (2.33 g/L) by 97% and butyric acid (2.36 g/L) by 143% compared with those obtained in the single step fermentation without any treatment. As a result, the H2 energy recovery efficiency of the two-step fermentation process with commercially attractive butyric acid as a coproduct reached 10.54%. These results provide a promising approach for sweet sorghum biorefining.

Ab initio theoretical simulations are performed for charge state distribution and energy loss of a 100 keV proton beam propagating in partially ionized discharge H plasmas. In the simulation, all transition processes are calculated accurately among eight main projectile electronic configurations including the most relevant excited states. Projectile configuration evolution is computed by solving rate equations, and the effective charge state Zeff is obtained during all discharge stages. A new way is suggested to get the projectile energy loss with the contributions of all configurations considered separately. Such obtained energy losses are in the best agreement with experimental measurements among all the related models. However, both traditional empirical models with estimated Zeff and the standard stopping model are found invalid in this case, and relevant reasons are explored. Projectile capture and ionization processes in plasmas are found to be especially important in the stopping process. We predict that projectile excited states would make a much larger difference in energy deposition when a higher atomic number projectile is chosen, which has not been deeply recognized in plasmas yet.

The purpose of the present study was to evaluate intra-articular ozone injection following arthroscopic surgery for knee osteoarthritis (OA) with regard to its efficacy in pain reduction, joint function and quality of life improvement. The present study retrospectively evaluated 80 patients with symptomatic knee OA (Kellgren-Lawrence grade II or III), who either did or did not receive 20 ml of 20 µg/ml ozone as an intra-articular injection after arthroscopic surgery. The minimum follow-up period was 12 months. The outcomes evaluated for knee OA were pain on the Visual Analogue Scale (VAS), Lequesne Index, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and Clinical Global Impression (CGI). The VAS score in the ozone group was significantly better than that in the control group at all post-operative follow-up time-points (P

2,5-Furandicarboxylic acid (FDCA) is a bio-based platform chemical for the production of polyethylene furanoate (PEF) and other valuable furanic chemicals. A magnetic laccase catalyst with (2,2,6,6-tetramethyl-piperidin-1-yl)oxyl (TEMPO) as the mediator has the remarkable capability of oxidizing 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA). Under optimal reaction conditions, a quantitative yield (90.2 %) of FDCA with complete HMF conversion was obtained after 96 h of reaction. More importantly, the magnetic laccase catalyst exhibited good recyclability and stability, maintaining 84.8 % of its original activity following six reuse cycles. This is the first report on the efficient catalytic oxidation of HMF to FDCA by using an immobilized enzyme catalyst.

Calycosin is widely used as a natural active compound for its anti-oxidative and anti-inflammation activity. Recently, several studies have shown that calycosin can inhibit growth and induce apoptosis in human cancer cell lines; however, the mechanisms are not completely clarified yet. In this study, we investigated the effects of calycosin on human ovarian carcinoma SKOV3 cells, as well as the mechanisms. SKOV3 cells were treated with calycosin at a series of concentrations for different times. In vitro, the MTT assay showed that calycosin had obvious anti-proliferation effects on SKOV3 cells in a dose- and time-dependent manner. Cell morphological changes which expressed by Hoechst 33258 staining were compared with apoptotic changes detected by fluorescence microscope. Compared with control group, the group treated with calycosin showed a significant increase in apoptosis rate. Expression of apoptosis related Bax/Bcl-2 and caspases proteins were detected by Western blotting. The results demonstrated that calycosin up-regulated the Bax/Bcl-2 ratio and cleaved caspase-3, cleaved caspase-9 expression in a dose-dependent manner. In summary, calycosin might exert anti-growth and induce-apoptosis activity against ovarian cancer SKOV3 cells through activating caspases and Bcl-2 family proteins, therefore presenting as a promising therapeutic agent for the treatment of ovarian cancer.

For raising the antigenicity ofMycobacterium bovissingle antigen, fusion protein of two genes was acquired. The DNA fragments ofmpb83andmpb70were fused by splicing by overlapping extension (SOE) polymerase chain reaction (PCR), and the fusion gene mpb83-mpb70 were cloned into pMD18-T vector, then we got the recombinant plasmid pMD-83-70. pMD-83-70 and pET28a (+) were digested byBamH I andEcoR I double enzymes. The purified pMD-83-70 fusion gene was subcloned into the expression vector pET28a (+), and the prokaryotic expression vector pET-83-70 was constructed. Plasmid containing pET-83-70 was transformed into competenceEscherichia coliBL21 (DE3). The bacterium was induced by isopropyl-β-D-thiogalactoside (IPTG) and the lysates were loaded directly onto sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), approximately 41 kDa exogenous protein was observed on the SDS-PAGE. The protein was analyzed by using Western blotting, the results indicated that the protein was of antigenic activity ofM.bovis. These results could serve as a basis for further studies on the usefulness of the fusion gene and its expression product in the development of subunit vaccine and DNA vaccine against bovine tuberculosis.

The simple-supported beam bridge is a common structure form widely used in small and medium span bridges. When the span is longer, the maximum bending moment is accordingly bigger. The maximum positive and negative bending moment numerical of beam decreases obviously because of cantilever bridge with cantilever beams to reduce the amount of materials. This article analyzes the current commonly used cantilever bridge with large span. The two-span cantilever bridge is analyzed under various loads about the internal force according to the condition of the absolute value of the maximum positive and negative bending moment being equal. It carries on the contrast and analysis about simple-supported beam bridges and obtains the best location of the hinge in the cantilever bridge. Moreover, it provides some reference for the optimum design of similar bridges and projects.

Objective To investigate whether the effect of perindopril on ameliorating heart function is associated with inhibiting endoplasmic reticulum stress (ERS) signaling pathway. Methods Myocardial infarction (MI) models in male SD rats were reproduced by ligation of the left anterior descending coronary artery (LAD). Rats that survived post-MI within 24h were randomly divided into model group (n=8) and perindopril group (n=8). Eight sham-operated rats were used as controls which underwent the same surgical procedure except that the suture around the coronary artery was not tied. The rats in peridopril group were treated with peridopril in a dose of 2mg/(kg.d) for 4 weeks. The rats in model group and sham group received an equal amount of saline. At the end of 4 weeks, transthoracic echocardiogram was performed to measure the ventricular morphology and heart function, including left ventricular end systolic diameter (LVESD) and end diastolic diameter (LVEDD), as well as left ventricular ejection fraction (LVEF) and fraction shortening (FS). The expression levels of GRP78 and caspase-12 protein were assessed with immunohistochemistry or Western blotting. Results Compared with the sham-operated group, the LVEDD and LVESD significantly increased after 4 weeks of MI, while the EF and FS decreased in model group. Perindopril significantly inhibited ventricular hypertrophy and improved heart function (P

Solar energy application combined with solar shading of buildings can not only take advantage of solar energy initiatively, but also save energy passively. By analyzing the strategy and integrated design methods of combination between solar energy application and shading system design, combining the geographical position of construction and solar radiation, this paper rationally design the arrangement of solar module, discuss the methods how to combine solar system with roof, facade window as well as superficial sun-shading of buildings, so that it could propose on making effective use of solar module design, which has dual efficacy, promoting the integration of solar energy application and shading system design.

A trimeric lignin model compound composed of α-O-4 and β-O-4 linkages was prepared by the microwave-assisted synthesis, which consisted of three steps: (a) the synthesis of 3-methoxy-4-benzyloxyacetophenone (2) from acetovanillone (1), (b) the bromination of compound 2 to produce 3-methoxy-4-benzyloxy-α-bromoacetophenone (3), and (c) followed by a nucleophilic substitution of compound (3) to obtain 3-methoxy-4-benzyloxy-α-(3-methoxy-4-(1-propenyl)phenol)-acetophenone (4). The target product was characterized by MS, 1H NMR and 13C NMR spectroscopy. It was found that the trimeric compound synthesized can be used as a preferable lignin model compound because it contains guaiacyl structural unit (3-methoxy-4-hydroxy phenyl propane) linked by α-O-4 and β-O-4 linkages. In addition, under the conditions of microwave irradiation, the reaction time of each step is significantly reduced, and the selectivity of target product is greatly improved. The yields of each step and the overall sequence are 95.31%, 87.3%, 90.6% and 75.4% (95.31% × 87.3% × 90.6%), respectively.

RNA secondary structure prediction algorithms tend to have higher time and space complexity. Some swarm intelligence algorithms can be applied to RNA secondary structure prediction on the basis of stem regions combinatorial optimization. This paper presents a parallel discrete shuffled frog leaping algorithm (parallel-DSFLA), which can run on a distributed cluster using the MPI programming mode. The experimental results show that the parallel-DSFLA got better speed-up ratio, can improve the RNA secondary structure prediction efficiency and save time.

We investigated whether the implantation of juvenile allograft and minced meniscal fragments could improve the healing of avascular meniscal injuries, which cannot heal spontaneously. Concentric cylindrical explants were excised from the inner two-thirds of swine medial menisci. The inner cylinder consisted of a "sandwich" structure, with minced juvenile meniscal fragments, juvenile meniscal columns, minced mature meniscal fragments, or mature meniscal columns implanted in the middle. The explants were cultured in vitro for 2, 4, or 6 weeks. Interfacial meniscal repair was assessed by histology, immunohistochemistry, biomechanical testing, and confocal laser scanning microscopy. Histology and confocal microscopy results revealed that tissue repair and cell accumulation at the interface were best at all time points in the juvenile meniscal fragments group, followed by the juvenile columns, minced mature fragments, and mature columns groups, respectively. At 6 weeks, the implantation of juvenile allograft and minced meniscal fragments increased the shear strength, peak force, and energy to failure in the peripheral interface. Picosirius red/polarized light microscopy and immunohistochemistry results showed concurrent expression of type I and II collagen in the interfacial repair tissue. In conclusion, implantation of juvenile allograft and minced meniscal fragments could increase the healing of avascular meniscal injury in vitro.

In the process of bogie separation device operations, rotating system is required to push bogie and settlement beam to rotate . After the completion of bogie wheel replacement and maintenance, they need to be pushed back to original position. This paper explains working principle of rotating mechanism of monorail vehicle bogie separation device and performs co-simulation and analysis of rotating mechanism based on ADAMS/MATLAB. Analysis results show that the rotating hinge mechanism and its proportional control system can completely meet the requirements of the rotary motion.

Logging measurement is the comprehensive reflection of solid particles and liquids in formation. Low energy sedimentary environment with weak water power and high salinity of formation water are the main requirements of low-resistance oil layer development. Whereas part of formation penetrated by hydrocarbon migration, which shows larger bend degrees of conductive tunnels in formation and higher resistivity measurement due to the oxidization of the residual crude oil, forms high-resistance water layer. Because of the complexity of accumulation conditions, these two difficult reservoirs exist in Putaohua reservoir of Weixing oilfield widely. Using geological characteristic parameters, such as, the distance of wells and scale faults, the formation structural location, resistivity measurement, sedimentary microfacies,thickness and porosity conditions, to evaluate oil and water property of the two difficult reservoirs, good results have been obtained.

The energy absorption characteristics of foam materials can be represented by specific energy absorption W, energy absorption efficiency E and ideal energy absorption efficiency I. The influences of strain rate, cell size, relative density and the content of SiC on energy absorption characteristics of magnesium alloy foam were investigated by dynamic compression experiments. The experimental results showed that the cell size of magnesium alloy foam had significant effect on energy absorption characteristics and the strain rate effect of closed-cell AZ91 magnesium with larger cell size was more sensitive, and the strain rate effect was more sensitive to the closed-cell AZ91 magnesium with larger cell size. The energy absorption characteristics could be optimized by adjusting the cell size. Moreover, the influence of relative density on energy absorption characteristics was not significant and high content of SiC particle had unfavorable effect on energy absorption properties of magnesium foam.

As the lack of topsoil resources, and the shortage of water resources, the ecological environment is extremely fragile in the pasture opencast coal mine area of China. Therefore, that the way to achieve the most efficient use of the limited topsoil resources in pasture area, plays a crucial role in land reclamation and ecological restoration. East II opencast coal mine in Datang, Inner Mongolia was taken as an example to analyze topsoil quality variation under different dump platform and slope reclamation modes in the pasture opencast coal mine area. The field investigation method was used in this paper. The study found that the mode "covering topsoil, overlying grass, grass grid, N fertilization, planting vanguard vegetation" in dump platform and "covering topsoil, overlying grass, grass grid, planting vanguard vegetation " in dump slope can lead to the best topsoil quality, though it still less than the original landscape topsoil quality level. The research can provide technical support and scientific basis for the pasture reclamation and ecological restoration.

In this study, 26 candidate genes were quantified and normalized in the brain cDNA of common carp (Cyprinus carpio) at 23°C and 6°C using double-standard curve method of real-time quantitative PCR. The results showed that five candidates up-regulated in the samples at 6°C (P 0.05), and their expression levels were decreased by 21.8%, 25.9%, 16.6%, 23.7%, 15.8%, 16.3%, and 42.5%, respectively, in comparison with the samples at 23°C. These seven down-regulated candidates mainly participated in the inhibition of glycolysis, improvement of cell apoptosis, and intervention of synapse remodeling based on the results of function searching. The five cold-induced genes identified in this study will be used as important elements for fish with cold sensitive through transgenic technology in future.

The titanium and its alloys are getting used more and more as for human implant in recent years. In this paper, the broken titanium palmer-bone implant was investigated with field emission scanning electron microscopy (SEM) and X-ray spectrometer and then the reasons of implant fracture was researched. The material composite analysis result shows that the implant is made of industrially pure titanium in which the microelements aluminum, calcium and vanadium are on the high sides. The macro analysis result indicates that the implant did not deform remarkably before cracking. The micro analysis result indicates that there is coarse crystalloid fracture in the SEM macrograph, suggesting that the implant material has low fracture strength. The implant fails in mixed cleavage fracture and intergranular fracture accompanied by fatigue fracture in part region, suggesting that the main fracture type was brittle fracture.

Implicit cost (PONC) which was produced by nonconformance in manufacturing process bring a large number of loss to the enterprise. For the data of PONC during manufacturing process is difficult to collect, and difficult to calculate costing of scrap and rework PONC, the included projects and the calculation method of Price of Nonconformance is proposed and analyzed to quantify scrap and rework PONC. The calculation method can be programmed and implemented by means of C# language in the paper, and the working procedure which the Price of Nonconformance is beyond the standard is analyzed and improved. The results of example analysis and verification show the calculation method of PONC is effective way to improve the efficiency of cost management, reduce quality cost, improve product quality and increase the economic efficiency of enterprise.