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7 results on '"Chun-Tian Zheng"'

1. Effect of heat stress on ileal microbial community of indigenous yellow‐feather broilers based on 16S rRNA gene sequencing

Author

Yong‐Yan Jin, Yan Guo, Chun‐Tian Zheng, and Wen‐Chao Liu

Subjects
16S rRNA sequencing, heat stress, ileal microbial community, yellow‐feather broilers, Veterinary medicine, SF600-1100
Abstract

Abstract Objectives The broiler chickens are susceptible to heat stress (HS), including the indigenous broilers raised in tropical and subtropical regions. HS caused intestinal dysfunction and disrupted the gut microbiota. However, the researches about the effects of HS on ileal microbiome of indigenous broilers are limited. Therefore, this experiment used 16S rRNA sequencing to analyse the ileal microbial community in indigenous yellow‐feather broilers under HS. Material and methods The single factor completely random design was used in the present study, and forty 8‐week‐old Chinese indigenous yellow‐feather broilers (Huaixiang chickens) were randomly divided into two treatments: normal temperature (NT) group and HS group. There are five replications with four broilers per replicate in each group. The broilers in NT group were raised at 21.3 ± 1.2°C during the whole experimental period, the broilers in HS group were exposed to 32.5 ± 1.4°C for 8 h/day from 9:00 am to 17:00 pm and the temperature of rest time is consistent with NT group. The experiment lasted for 4 weeks. Results The results showed that HS exposure had no significant effects on the alpha diversity index of ileal microflora of broilers, including the Shannon, Simpson, Chao1 and ACE indexes (p > 0.05). At the genus level, HS significantly reduced the relative abundance of Campylobacter (p

Published
2022
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2. Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal sIgA secretions and gut microbial populations in weaned piglets

Author

Cui ZHU, Li WANG, Shao-yong WEI, Zhuang CHEN, Xian-yong MA, Chun-tian ZHENG, and Zong-yong JIANG

Subjects
Saccharomyces cerevisiae, antioxidant capacity, sIgA, intestinal health, gut microbiota, weaned piglets, Agriculture (General), S1-972
Abstract

This study was conducted to determine the effect of different forms of yeasts Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal secretory immunoglobulin A (sIgA) secretions and gut microbial populations in weaned piglets. A total of 96 piglets weaned at 14 d of age were randomly allotted to 4 dietary treatments: (1) basal diet without yeast (Control); (2) basal diet supplemented with 3.00 g kg−1 live yeast (LY); (3) basal diet supplemented with 2.66 g kg−1 heat-killed whole yeast (HKY); and (4) basal diet supplemented with 3.00 g kg−1 superfine yeast powders (SFY). Each treatment had 4 replicates (pens), with 6 piglets per replicate. The experiment lasted for 3 wk. At d 7 and 21 of the experiment, the samples of serum, mucosa and mesenteric lymph node (MLN) from jejunum, and digesta from the ileum and cecum were collected for determinations. Compared with the Control, dietary SFY supplementation increased serum superoxide dismutase (SOD) activity and lysozyme levels at d 7, and jejunum mucosal slgA secretions at d 21 of the experiment (P

Published
2017
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3. Effects of Dietary Isoflavone Supplementation on Meat Quality and Oxidative Stability During Storage in Lingnan Yellow Broilers

Author

Shou-qun JIANG, Zong-yong JIANG, Gui-lian ZHOU, Ying-cai LIN, and Chun-tian ZHENG

Subjects
Lingnan yellow broilers, isoflavone, meat quality, oxidative stability, glutathione peroxidase mRNA, Agriculture (General), S1-972
Abstract

To investigate the effects of a synthetic isoflavone (SI) on meat quality and oxidative stability, 1 500 43-d-old Lingnan yellow male broilers were randomly assigned to five dietary treatments: diets supplemented with 0, 10, 20, 40, or 80 mg SI kg−1, fed ad libitum for a period of 3 wk. After refrigerated storage, a* value of the meat increased with dietary SI supplementation using 10 and 40 mg kg−1 level (P

Published
2014
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4. Marinobacter shengliensis subsp. alexandrii Subsp. Nov., Isolated from Cultivable Phycosphere Microbiota of Highly Toxic Dinoflagellate Alexandrium catenella LZT09 and Description of Marinobacter shengliensis Subsp. shengliensis Subsp. Nov

Author

Jia-Zhou Li, Nurhezreen Md Iqbal, Shen-Quan Liao, Rong Xiang, Xi Yang, Lin-Zeng Yu, Qiao Yang, Xiao-Ai Zhang, Chun-Tian Zheng, Lei Wang, Ming-Fei Sun, and Yu-Han Duan

Subjects
DNA, Bacterial, Alexandrium catenella, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Phylogenetics, RNA, Ribosomal, 16S, Marinobacter, medicine, Paralytic shellfish poisoning, Phylogeny, 030304 developmental biology, 0303 health sciences, Base Composition, biology, 030306 microbiology, Microbiota, Fatty Acids, Dinoflagellate, Nucleic Acid Hybridization, General Medicine, Sequence Analysis, DNA, Ribosomal RNA, biology.organism_classification, 16S ribosomal RNA, medicine.disease, Bacterial Typing Techniques, Dinoflagellida, Marinobacter shengliensis
Abstract

Phycosphere hosts the boundary of unique holobionts harboring dynamic algae-bacteria interactions. During our investigating the microbial consortia composition of phycosphere microbiota (PM) derived from diverse harmful algal blooms (HAB) dinoflagellates, a novel rod-shaped, motile and faint yellow-pigmented bacterium, designated as strain LZ-6 T, was isolated from HAB Alexandrium catenella LZT09 which produces high levels paralytic shellfish poisoning toxins. Phylogenetic analysis based on 16S rRNA gene and two housekeeping genes, rpoA and pheS sequences showed that the novel isolate shared the highest gene similarity with Marinobacter shengliensis CGMCC 1.12758 T (99.6%) with the similarity values of 99.6%, 99.9% and 98.5%, respectively. Further phylogenomic calculations of average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strains LZ-6 T and the type strain of M. shengliensis were 95.9%, 96.4% and 68.5%, respectively. However, combined phenotypic and chemotaxonomic characterizations revealed that the new isolate was obviously different from the type strain of M. shengliensis. The obtained taxonomic evidences supported that strain LZ-6 T represents a novel subspecies of M. shengliensis, for which the name is proposed, Marinobacter shengliensis subsp. alexandrii subsp. nov. with the type strain LZ-6 T (= CCTCC AB 2018388TT = KCTC 72197 T). This proposal automatically creates Marinobacter shengliensis subsp. shengliensis for which the type strain is SL013A34A2T (= LMG 27740 T = CGMCC 1.12758 T).

Published
2020

5. Dietary calcium deficiency in laying ducks impairs eggshell quality by suppressing shell biomineralization

Author

Wei, Chen, Fei, Zhao, Zhi Mei, Tian, Han Xing, Zhang, Dong, Ruan, Yan, Li, Shuang, Wang, Chun Tian, Zheng, and Ying Cai, Lin

Subjects
Estradiol, Tibia, Oviposition, Egg Proteins, Uterus, Calcium, Dietary, Egg Shell, Calcification, Physiologic, Ducks, Calbindin 1, Animals, Animal Nutritional Physiological Phenomena, Calcium, Female
Abstract

The objective of this study was to determine the effects of dietary calcium deficiency on the process of shell formation. Four hundred and fifty female ducks (Anas platyrhynchos) at 22 weeks were randomly assigned to three groups. Ducks were fed one of two calcium-deficient diets (containing 1.8% or 0.38% calcium, respectively) or a calcium-adequate control diet (containing 3.6% calcium) for 67 days (depletion period) and then all ducks were fed a calcium-adequate diet for an additional 67 days (repletion period). Compared with the calcium-adequate control, the average shell thickness, egg shell weight, breaking strength, mammillae density and mammillary knob thickness of shell from ducks that consumed the diet with 0.38% calcium were significantly decreased (P0.05) during the depletion period, accompanied by reduced tibia quality. The mRNA expression of both secreted phosphoprotein 1 (SPP1) and carbonic anhydrase 2 (CA2) in the uterus was decreased after feeding calcium-deficient diets (1.8% or 0.38% calcium). mRNA transcripts of calbindin 1 (CALB1), an important protein responsible for calcium transport, and the matrix protein genes ovocalyxin-32 (OCX-32) and ovocleidin-116 (OC-116) were reduced in ducks fed 0.38% calcium but not 1.8% calcium. Plasma estradiol concentration was decreased by both of the calcium-deficient diets (P0.05). The impaired shell quality and suppressed functional proteins involved in shell formation could be reversed by repletion of dietary calcium. The results of the present study suggest that dietary calcium deficiency negatively affects eggshell quality and microarchitecture, probably by suppressing shell biomineralization.

Published
2015

6. Dietary calcium deficiency in laying ducks impairs eggshell quality by suppressing the process of shell biomineralization

Author

Chun Tian Zheng, Zhi Mei Tian, Dong Ruan, Wei Chen, Yan Li, Fei Zhao, Han Xing Zhang, Ying Cai Lin, and Shuang Wang

Subjects
medicine.medical_specialty, biology, Physiology, Uterus, Shell (structure), Egg protein, chemistry.chemical_element, Aquatic Science, Calcium, Calbindin, medicine.anatomical_structure, Endocrinology, chemistry, Insect Science, Carbonic anhydrase, Internal medicine, medicine, biology.protein, Animal Science and Zoology, Eggshell, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Biomineralization
Abstract

The objective of this study was to determine the effects of dietary calcium deficiency on the process of shell formation. Four hundred and fifty female ducks (Anas platyrhynchos) of 22 wk were randomly assigned to 3 groups. Ducks were fed one of two calcium-deficient diets (containing 1.8% or 0.38% calcium, respectively) or a calcium-adequate control diet (containing 3.6% calcium) for 67 d (depletion period), and then ducks of the 3 groups were fed a calcium-adequate diet for an additional 67 d (repletion period). As compared with the calcium-adequate control, the average shell thickness, egg shell weight, breaking strength, mammillae density and mammillary knob thickness of shell from ducks that consumed the diet with 0.38% calcium was significantly decreased (P

Published
2015

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