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Introduction Secondary brain injury is the main cause of mortality from traumatic brain injury (TBI). One hallmark of TBI is intracranial hemorrhage, which occurs in 40-50% of severe TBI cases. Early identification of intracranial hematomas in TBI patients allows early surgical evacuation and can reduce the case fatality rate of TBI. As pre-hospital care is the weakest part of Chinese emergency care, there is an urgent need for a capability to detect brain hematomas early. In China, in addition to preventing injuries and diseases in military staff and in enhancing the military armed forces during war, military medicine participates in actions such as emergency public health crises, natural disasters, emerging conflicts, and anti-terrorist campaigns during peacetime. The purpose of this observational study is to evaluate in the Chinese military general hospital the performance of a near-infrared (NIR)-based portable device, developed for US Military, in the detection of traumatic intracranial hematomas. The endpoint of the study was a description of the test characteristics (sensitivity, specificity, and positive and negative predictive values [NPV]) of the portable NIR-based device in identification of hematomas within its detection limits (volume >3.5 mL and depth

Amplified fragment length polymorphism (AFLP) analyses were used to assess genetic diversity among 30 genotypes of watermelon [Citrullus lanatus (Thunb.) Mansf.] representing a broad genetic base, including breeding lines and commercial germplasm. Eight AFLP primer combinations selected from 64 primer combinations were polymophic. The polymorphism was 13.0% to 31.9% within the 28 cultivars examined, and 45.3% to 64.2% among all the genotypes. Each genotype could be successfully distinguished based on AFLP scoring. Cluster grouping of accessions based on the AFLP analysis was consistent with that from classification by pedigrees and ecotypes.

To explore the expression and changes of protooncogene c-myc in early restenosis after carotid endarterectomy (CEA).Using New Zealand Rabbits, carotid atherosclerotic stenosis (CASS) model were created. The expression of c-myc in early restenosis after CEA were detected by quantitative real-time polymerase chain reaction (qRT-PCR).In this experiment protooncogene c-myc was analysed at pre-operation, 4 hours and 1 d after CEA by qRT-PCR, and the level of mRNA of c-myc was reached maximum at 4 hours, and decreased following significantly, but still higher than pre-operation.The activation of protooncogene c-myc may be generator factor of vascular smooth muscle cell proliferation.

The thermo-sensitive point and the thermo-sensitive stage for fertility alteration in the thermo-sensitive genic male sterile (TGMS) line Annong S-1 were studied. In nature environment and green house,the leaf, root and young panicle were treated by low and high temperature respectively. In total, 8 treatments were carried out. Results indicated that during the thermo-sensitive stage of fertility alteration, in high temperature environmental conditions, the low temperature treatment in the root did not significantly induce Annong S-1 to be fertile, while the low temperature treatment in young panicle obviously induced Annong S-1 to be fertile. Therefore, the thermo-sensitive point of Annong S-1 is the young panicle. The stage of fertility alteration was also investigated. Results indicated that alteration occurred in the phase from the formation of pollen mother cell to the tetrad of miosis. aprt gene is related the male sterility. The expression of aprt gene in root,young panicle and leaf were investigated by RT-PCR respectively. In young panicle, the expression of aprt gene was greatly down regulated by high temperature, while in root and leaf the expressions have no great changes. These data show that the young panicle is sensitive to temperature change. The result also supported that the young panicle is the thermo-sensitive point of Annong S-1 in the fertility alteration. These conclusions can be used to direct in hybrid rice seed production.

Southern corn rust (SCR) is a destructive disease in maize. The inbred line Qi319 is highly resistant to southern corn rust. SSR technique was employed to preliminary mapping of the resistance gene. Bulked segregant analysis revealed that two primers, phi 118 and phi 041, amplified polymorphic bands. SSR analysis on populations indicated the two primers were linked to the rust resistance gene, which was mapped on the short arm of chromosome 10. In addition, comparative analysis of the amplification bands among different populations revealed that the amplification products with the same primer in different populations were dissimilar. This result indicates that the genetic background may affect results of gene mapping and tagging. So, it is important to select suitable population to performing molecular marker analysis and gene mapping.