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Summary: Background: Non-invasive neuromodulation is a promising approach for improving spasticity and motor function after stroke. However, it is still unclear which type of non-invasive neuromodulation is effective and evidence of important differences between them and botulinum toxin (BoNT) injection is limited. We aimed to assess the comparative efficacy and acceptability of non-invasive neuromodulation technologies and BoNT for post-stroke spasticity and motor function. Methods: In this network meta-analysis, Cochrane Library, EMBASE, MEDLINE, Web of Science, Scopus, CNKI, and Wan Fang Data were searched from the earliest records to 8 October 2024. Randomised controlled trials that compared any type of non-invasive neuromodulation therapies, BoNT, and control treatments (including sham or no stimulation/injection) for post-stroke spasticity measured by modified Ashworth scale (MAS) were included. MAS, motor function, and acceptability were pooled using random-effects model with summary weighted mean difference (WMD) or risk ratios (RR) alongside 95% confidence interval (CI). Ranking probabilities of the treatments were estimated. Clinical importance was categorized as definite, probable, possible, or definitely not, considering the relationship between effect measures (95% CI) and minimal clinically important difference (1, 6, and 1.5 points for MAS, motor function, and acceptability, respectively). The quality of evidence was assessed using CINeMA online web. PROSPERO registration CRD42024543494. Findings: 6260 studies were identified and 185 trials (11,185 participants; 12 interventions) were included. Compared with control treatments, BoNT, high- and low-frequency repetitive transcranial magnetic stimulation (HFrTMS and LFrTMS), and anodal, cathodal, and dual transcranial direct current stimulation (atDCS, ctDCS, and dtDCS) significantly improved spasticity at short-term follow-up (WMD range −0.81 to −0.31), but did not achieve clinical importance. At mid-term, ctDCS (WMD = −2.00; 95% CI: −3.03, −0.97) and dtDCS (WMD = −1.62; 95% CI: −3.22, −0.02) were more efficacious than control treatments in reducing post-stroke spasticity with probable clinical importance. For motor function, atDCS, ctDCS, and dtDCS were more efficacious than control treatments (WMD range 6.29–13.00), with probable clinical importance, while BoNT, HFrTMS, and LFrTMS with possible clinical importance (WMD range 3.42–5.28). Various modalities have comparable acceptability to control treatments (RR range 0.48–1.46). Confidence in accordance with CINeMA ranged from high to low. Sensitivity and meta-regression analyses on limb measured, cointervention, and stroke stage confirmed the main findings of this study. Interpretation: Taken together with clinical importance, evidence available supports three forms of tDCS as effective treatments for post-stroke spasticity and/or motor impairments, whereas BoNT, HFrTMS, and LFrTMS for motor impairments. These modalities could be considered alongside rehabilitation interventions as core treatments for post-stroke spasticity and motor impairments. Funding: China Postdoctoral Science Foundation (2024M752230).

Based on the overall configuration characteristics of the terahertz detection module and the thermal environmental conditions during the detection mission, particularly the thermal control challenges such as multiple heat sources, high heat dissipation, and compact internal component installation, an effective thermal control design scheme has been proposed. A comparative analysis of the simulation and thermal balance test results of the terahertz detection module under extreme low and extreme high temperature conditions were conducted. The thermal balance test results indicate that the temperatures of the terahertz detection module's framework and internal components meet the thermal control requirements, with the deviation between simulated and tested temperatures being less than 2.8 °C. This indicates that the thermal analysis of the terahertz detection module is reasonable and effective, and the thermal design is correct and feasible. To address the issues of uneven temperature distribution on the heat dissipation plate and the high local temperature of the compressor unit, an improvement measure has been proposed to fill the lightweight grooves of the module's heat dissipation plate. Simulation analysis shows that this measure is effective to some extent. This research provides an important theoretical foundation and research experience for the thermal design of space probe with multiple heat sources and high heat dissipation.

The historical appreciation of tea dates back to ancient times, while technological limitations have long hindered in-depth exploration of its flavor complexity and functional attributes. This study investigated the effects of various teas on a traditional delicacy, “tea-flavored fish”, using teas processed via traditional methods. Analysis of functional components revealed that processing and fermentation reduced catechin levels (186.3 mg/g to 58.8 mg/g) while increasing theaflavins (16.6 mg/g to 39.6 mg/g), leading to decreased antioxidant and antimicrobial activities. Tea flavored fish was prepared following traditional techniques. The results indicated that the teas preserved their sensory qualities such as texture and color, inhibited metabolic activity and microbial growth, delayed lipid oxidation and protein degradation, and inhibited biogenic amine accumulation. Furthermore, minor compositional variations were observed in the final product. These findings offer novel insights into the application of modern scientific concepts to elucidate the principles underlying traditional craftsmanship.

The camera mounted at the end of the space robot arm provides real-time visual monitoring and positioning functions during space missions. Its thermal design is crucial for ensuring both functionality and performance, particularly in challenging environments characterized by high radiation, extremely low temperatures, and vacuum conditions. Effective thermal management guarantees stable camera operation despite temperature fluctuations, ensuring both image quality and functional reliability. The integrated thermal design incorporates both passive and active controls, including cooling surfaces, multilayer insulation components, light shields, and heating loops. Simulation and experimental results show that, under low-temperature conditions, the active temperature control system effectively maintains the camera housing temperature above −50 °C, validating the stability of the thermal design. In high-temperature conditions, the camera housing temperature rises by 30 °C, reaching approximately 0 °C, confirming its efficient heat dissipation capability. Furthermore, after approximately 100 orbital cycles, the camera consistently stabilizes within the designed temperature range, affirming the precision and reliability of the thermal design. These findings offer essential insights for optimizing thermal management strategies in space cameras intended for complex space missions.

BackgroundProgress in immunotherapy for pancreatic ductal adenocarcinoma (PDAC) has been slow, yet the relationship between microorganisms and metabolites is crucial to PDAC development. This study compares the biliary microbiota and metabolomic profiles of PDAC patients with those of benign pancreatic disease patients to investigate PDAC pathogenesis and its relationship with immunotherapy.MethodsA total of 27 patients were recruited, including 15 diagnosed with PDAC and 12 with benign pancreaticobiliary conditions, all of whom underwent surgical treatment. Intraoperative bile samples were collected and analyzed using 16S rRNA sequencing in conjunction with liquid chromatography-mass spectrometry (LC-MS). Multivariate statistical methods and correlation analyzes were employed to assess differences in microbial composition, structure, and function between malignant and benign pancreatic diseases. Additionally, a retrospective analysis was conducted on PDAC patients post-surgery regarding immunotherapy and its correlation with metabolic components.ResultsPDAC patients exhibited a significantly higher abundance of bile microbiota compared to controls, with notable differences in microbiota structure between the two groups (P < 0.05). At the genus level, Muribaculum was markedly enriched in the bile of PDAC patients and was strongly correlated with phosphatidic acid (PA) (10:0/a-17:0). Both of these components, along with the tumor marker CA199, formulated a predictor of PDAC. Furthermore, PA (10:0/a-17:0) demonstrated a strong correlation with PDAC immunotherapy outcomes (Rho: 0.758; P=0.011).ConclusionThese findings suggest that the biliary microbiota and associated metabolites play a crucial role in the development of PDAC and may serve as potential predictive biomarkers and therapeutic targets for disease management.

Non-native freshwater fish species are regarded as a key factor responsible for the degradation of freshwater ecosystems. Although research on China's non-native freshwater fish species has been conducted at the national scale, the spatial distribution and introduction pathways of these species in China remain unclear. To address this knowledge gap, this study compiled a dataset of non-native freshwater fish species across administrative regions in China. We first assessed the number of non-native freshwater fish species by taxonomy, geographical origin, introduction pathway, and province. There were 177 non-native freshwater fish species, belonging to 17 orders, 48 families, and 118 genera. The orders Cypriniformes (33.3%), Perciformes (28.2%), Siluriformes (10.2%), and Salmoniformes (6.2%) accounted for the largest proportion of non-native freshwater fish species. Eighty-nine non-native species were introduced from other countries or regions, mostly from North America (31 species; 34.8%), Asia (20 species; 22.5%), Africa (13 species; 14.6%), Europe (11 species; 12.4%), and South America (10 species; 11.2%). Aquaculture was the most common introduction pathway. Non-native freshwater fish species were more widely distributed in southwest China. Our study showed that there were obvious differences in the number and composition of non-native freshwater fish species across various provinces in China. The variation in the number of non-native freshwater fish species across provinces in China was attributed to distinct geographical features, development of the aquaculture industry, and efforts to study non-native freshwater fish species. Therefore, comprehensive surveys and studies of non-native freshwater fish species are needed, which are of great importance for the management and control of non-native species invasions.

Abstract Invasive alien species are one of the major threats to global biodiversity, ecosystem integrity, nature's contributions to people and human health. While scenarios about potential future developments have been available for other global change drivers for quite some time, we largely lack an understanding of how biological invasions might unfold in the future across spatial scales. Based on previous work on global invasion scenarios, we developed a workflow to downscale global scenarios to a regional and policy‐relevant context. We applied this workflow at the European scale to create four European scenarios of biological invasions until 2050 that consider different environmental, socio‐economic and socio‐cultural trajectories, namely the European Alien Species Narratives (Eur‐ASNs). We compared the Eur‐ASNs with their previously published global counterparts (Global‐ASNs), assessing changes in 26 scenario variables. This assessment showed a high consistency between global and European scenarios in the logic and assumptions of the scenario variables. However, several discrepancies in scenario variable trends were detected that could be attributed to scale differences. This suggests that the workflow is able to capture scale‐dependent differences across scenarios. We also compared the Global‐ and Eur‐ASNs with the widely used Global and European Shared Socioeconomic Pathways (SSPs), a set of scenarios developed in the context of climate change to capture different future socio‐economic trends. Our comparison showed considerable divergences in the scenario space occupied by the different scenarios, with overall larger differences between the ASNs and SSPs than across scales (global vs. European) within the scenario initiatives. Given the differences between the ASNs and SSPs, it seems that the SSPs do not adequately capture the scenario space relevant to understanding the complex future of biological invasions. This underlines the importance of developing independent but complementary scenarios focussed on biological invasions. The downscaling workflow we implemented and presented here provides a tool to develop such scenarios across different regions and contexts. This is a major step towards an improved understanding of all major drivers of global change, including biological invasions. Read the free Plain Language Summary for this article on the Journal blog.

Abstract In the Anthropocene, non-native freshwater fish introductions and translocations have occurred extensively worldwide. However, their global distribution patterns and the factors influencing their establishment remain poorly understood. We analyze a comprehensive database of 14953 freshwater fish species across 3119 river basins and identify global hotspots for exotic and translocated non-native fishes. We show that both types of non-native fishes are more likely to occur when closely related to native fishes. This finding is consistent across measures of phylogenetic relatedness, biogeographical realms, and highly invaded countries, even after accounting for the influence of native diversity. This contradicts Darwin’s naturalization hypothesis, suggesting that the presence of close relatives more often signifies suitable habitats than intensified competition, predicting the establishment of non-native fish species. Our study provides a comprehensive assessment of global non-native freshwater fish patterns and their phylogenetic correlates, laying the groundwork for understanding and predicting future fish invasions in freshwater ecosystems.

Abstract Emergent plants have been remarkably effective in reducing phosphorus (P) discharge from ecological ditches; however, the treatment and recycling of these residues is a great challenge. In this study, magnetic biochars (MBs, i.e., MB-A, MB-C, and MB-T) were fabricated from three emergent plant residues (Acorus calamus L., Canna indica L., and Thalia dealbata Fraser, respectively) and modified with Fe(II)/Fe(III). Scanning electron microscopy-energy dispersive spectroscopy and X-ray diffraction spectra confirmed the successful loading of Fe3O4 and FeO(OH) onto the surfaces of the MBs. Batch adsorption experiments showed that MBs exhibited a higher P adsorption capacity than that of the raw biochars. Within the range of 0.8–43.0 mg L−1 in solution, the adsorption capacities of P by MB-A, MB-C, and MB-T were 304.6–5658.8, 314.9–6845.6, and 292.8–5590.0 mg kg−1, with adsorption efficiencies of 95.2–32.9%, 98.4–39.8%, and 91.5–32.5%, respectively. The primary mechanisms that caused P to adsorb onto the MBs were inner-sphere complexation and electrostatic attraction. Low pH conditions were more beneficial for the P adsorption of the MBs, while co-existing anions had a negative impact with the following order: HCO3 − > SO4 2− > Cl−≈NO3 −. The P-31 nuclear magnetic resonance results further demonstrated that the main adsorbed P species on the MBs was orthophosphate, followed by orthophosphate monoesters and DNA. Overall, MBs offer a resource utilization strategy for emergent plant residues and P-laden MBs are promising alternative P fertilizers. Graphical Abstract

The objective of this study was to assess the impact of Lactobacillus plantarum, Bacillus subtilis, and a lignocellulolytic enzyme system on the nutritional value, fermentation profiles, rumen digestion, and bacterial community of fresh waxy corn stalk silage. Fresh waxy corn stalks harvested after 90 days of growth were treated with no additives (CON), compound multiple lactobacilli (ML, comprising Lactobacillus plantarum at 1.0 × 105 cfu/g fresh weight and Bacillus subtilis at 1.0 × 105 cfu/g fresh weight), a lignocellulolytic enzyme system (LE, at 500 g/t), and a combination of the lignocellulolytic enzyme system and multiple lactobacilli (MLLE). Throughout the fermentation process, the contents of dry matter (DM) and water-soluble carbohydrates (WSC), as well as the counts of lactic acid bacteria, yeast, and mold, continuously decreased with the extension of the fermentation time. In contrast, the concentrations of acetic acid, propionic acid, and ammonia N progressively increased over time. Notably, the pH initially decreased and then increased as fermentation progressed, while the lactic acid concentration first increased and then decreased with the extension of the fermentation time. After 45 days of fermentation, the DM contents of LE and MLLE were significantly lower than that of CON, while the WSC content of MLLE was significantly higher than that of CON. LE most effectively increased the ED of DM and neutral detergent fiber (NDF). Compared to ML and LE, MLLE showed a greater increase in the “a” value of DM and NDF. The DM content in LE and MLLE was lower than in CON, with a higher WSC content. Both ML and MLLE produced more lactic acid than CON and LE, resulting in a lower pH in these groups. Additionally, the acetic acid content and ammonia N content were lower in ML and MLLE compared to CON and LE. The counts of lactic acid bacteria, yeast, and mold declined over the fermentation period, dropping below detectable levels on the seventh and fifth days, respectively. Relative to CON, the three additives reduced the relative abundance of Weissella and Klebsiella and increased that of Lactobacillus. In conclusion, LE improved the nutritional value and rumen digestion of the silage, ML enhanced its fermentation profiles and bacterial community, and MLLE significantly improved the nutritional value, rumen digestion characteristics, fermentation profiles, and bacterial community of the fresh waxy corn stalk silage.

Abstract An increasingly globalised world has facilitated the movement of non‐native species (NNS) via the poorly regulated international pet trade. While focus is increasingly being placed on preventative action to combat invasive NNS—often cheaper and less difficult than the management of established populations—successful prevention requires controlling potential pathways and obtaining baseline knowledge of species' availability. Here we performed an in‐depth analysis of the freshwater pet trade as one major vector of NNS, compiling its species inventory and deriving threats of NNS release and establishment in the wild. With Germany as our study region, we surveyed pet stores, websites and the country's largest online classified portal, eBay Kleinanzeigen, recording the taxa encountered. For each species, we determined the likelihood of release based on availability and price (cheaper and/or more readily available species have been shown to be of greater risk), and the likelihood of establishment based on ecological niche breadth and niche overlap with environmental conditions in Germany. The survey revealed 669 species, of which 651 were non‐native to Germany. Looking at release likelihood, more readily available species in pet stores and on websites proved to be cheaper. For websites, there was a significant effect of occurrence status (i.e. released, not released, native) on price, with released and native species being significantly cheaper. Species previously released in Germany and elsewhere demonstrated greater niche breadths and greater niche overlaps between their source regions and Germany; and for species released in Germany, there was a significantly positive relationship between the magnitude of niche overlap and the number of documented occurrences. Finally, we combined our release and establishment likelihood findings under ‘Release Risk’ metrics to highlight the species most worthy of prioritisation. We propose these metrics as proactive methods for screening species in the trade, which can inform future policy direction and intervention. Read the free Plain Language Summary for this article on the Journal blog.

Background: To describe the past, present and future burden of pancreatitis in older adults, and to explore cross-national inequalities across socio-demographic index (SDI). Methods: Data on pancreatitis in older adults, including mortality and disability-adjusted life years (DALYs) rates, were collected from the Global Burden of Disease (GBD) 2019 study. Temporal trends were measured using joinpoint analyses and predicted using a Bayesian age-period-cohort model. Additionally, the unequal distribution of the burden of pancreatitis in older adults was quantified. Results: From 1990 to 2019, the number of deaths and DALYs due to pancreatitis in older adults has been increasing annually. However, in most regions of the world, age-standardized death rates (ASDR) and age-standardized DALYs rates have been declining. The burden of pancreatitis in older adults was highest in low SDI region, primarily affecting the population aged 65–74, with a greater burden on males than females. Furthermore, from 1990 to 2019, absolute and relative cross-national inequalities in pancreatitis among older adults have remained largely unchanged. It is projected that in the next 11 years, the number of deaths in older adults due to pancreatitis will continue to increase, but the ASDR is expected to decline. Conclusion: Over the past 30 years, the ASDR and age-standardized DALYs rate of pancreatitis in older adults have shown a decline globally, but the absolute burden continues to increase. Cross-national health inequalities persist. Therefore, it is necessary to develop targeted intervention measures and enhance awareness among this vulnerable population regarding the risk factors associated with pancreatitis.

As predators are generally larger than their prey, positive body size and trophic position relationships are typically assumed, and these relationships have helped in estimating and predicting the effects of global environmental change on the trophic dynamics of aquatic ecosystems. However, current efforts to confirm the generality of body size-trophic position relationships have focused mainly on interspecific patterns using species-aggregated and averaged data, and little effort has been devoted to assessing how trophic position scales with body size at the intraspecific and community levels as well as exploring its ecological drivers, particularly for fishes in freshwater ecosystems. To fill this gap, we present a broad-scale study of body size-trophic position relationships in freshwater ecosystems at both the intraspecific and community levels using an individual-based body size and isotopic signature dataset that includes 2564 samples of 65 fish species in China. Our results indicate that body size-trophic position patterns at both the intraspecific and community levels can be positive, negative or insignificant. Non-significant patterns predominated at both levels, with slightly more than 30 % showing positive relationships, which highlights that the positive body size-trophic position pattern is not universally prevalent and that body size should be used with caution as a proxy for the trophic position of fishes at both the intraspecific and community levels in freshwater ecosystems. No factor was related to body size-trophic position patterns at the community level, while habitat type, elevation, temperature and fishing pressure were identified as the key determinants of body size-trophic position patterns at the intraspecific level, implying that fishing and climate warming can shift the food-web size structure of freshwater ecosystems by affecting population trophic dynamics.

Abstract Drying and rewetting (DRW) events cause the release of colloidal phosphorus (Pcoll, 1–1000 nm) in leachate, and biochar is considered an effective inhibitor; however, the microbial mechanism remains elusive. In this study, three successive DRW cycles were performed on the soil columns to assess the effect of biochar addition on Pcoll content and its possible associates, including phosphatase-producing microbial populations (phoD- and phoC-harboring microbial communities) and alkaline/acid phosphatase (ALP/ACP) activities. Results showed that the biochar addition significantly decreased the Pcoll by 15.5–32.1% during three DRW cycles. The structural equation model (SEM) confirmed that biochar addition increased phoD- and phoC-harboring microbial communities and ALP/ACP activities, which reduces the release of Pcoll into leachate. In addition, the manure biochar was more effective than the straw biochar in promoting competition and cooperation in the co-occurrence network (2–5% nodes increased on average), and the key taxa Proteobacteria and Cyanobacteria were identified as the dominant species of potential ALP/ACP activities and Pcoll content. Our findings provide a novel understanding of biochar reducing Pcoll loss from the phosphatase perspective by regulating the phoD- and phoC-harboring communities during DRW events. Graphical abstract

Purpose: To investigate the effect of isometric prone trunk extension (IPTE) contraction intensity on the stiffness of erector spinae (ES), semitendinosus (ST), biceps femoris (BF), and gastrocnemius muscles to understand the overall muscle mechanical behavior during IPTE and to explore the mechanisms of oordinated contraction of the body kinetic chain.Methods: Twenty healthy females were recruited, and participants underwent IPTE at three contraction intensities, i.e., 0% maximum voluntary isometric contraction (MVIC), 30% MVIC, and 60% MVIC, and muscle stiffness was measured using MyotonPRO.Results: Muscle stiffness was moderately to strongly positively correlated with contraction intensity (r = 0.408–0.655, p < 0.001). The percentage increase in stiffness at low intensity was much greater in ES than in lower limb muscles and greater in ST and BF than in gastrocnemius, whereas at moderate intensity, the percentage increase in stiffness decreased in all muscles, and the percentage increase in stiffness in ES was lower than that in ST. There was a moderate to strong positive correlation between ES stiffness variation and ST (r = 0.758–0.902, p < 0.001), BF (r = 0.454–0.515, p < 0.05), MG (r = 0.643–0.652, p < 0.01), LG (r = 0.659–0.897, p < 0.01).Conclusion: IPTE significantly affected the stiffness of lumbar and lower limb muscles, and low-intensity IPTE activated the ES more efficiently. There were significant coordinated muscle contractions between ES, ST, and LG. This provides preliminary evidence for exploring the overall modulation pattern of the lumbar and lower limb muscles’ kinetic chains. In future studies, we will combine other stiffness assessment methods (such as Magnetic Resonance Elastography, Shear Wave Elastography, or electromyography) to corroborate our findings.

With advances in medicine, increasing medical interventions have increased the risk of invasive fungal disease development. (1-3)-β-D glucan (BDG) is a common fungal biomarker in serological tests. However, the scarcity of Limulus resources for BDG detection poses a challenge. This study addresses the need for an alternative to Limulus amebocyte lysate by using BDG mutant antibody for chemiluminescence detection. The wild-type BDG antibody was obtained by immunizing rabbits. An optimal V52HI/N34L Y mutant antibody, which has increased 3.7-fold of the testing efficiency compared to the wild-type antibody, was first achieved by mutating “hot-spot” residues that contribute to strong non-covalent bonds, as determined by alanine scanning and molecular dynamics simulation. The mutant was then applied to develop the magnetic particle chemiluminescence method. 574 clinical samples were tested using the developed method, with a cutoff value of 95 pg/mL set by Limulus amebocyte lysate. The receiver operating characteristic curve demonstrated an area under the curve of 0.905 (95% CI: 0.880–0.929). Chemiluminescence detected an antigen concentration of 89.98 pg/mL, exhibiting a sensitivity of 83.33% and specificity of 89.76%. In conclusion, the results showed a good agreement with Limulus amebocyte lysate and demonstrated the feasibility of using BDG mutant antibodies for invasive fungal disease diagnosis. The new method based on chemiluminescence for detecting BDG could shorten the sample-to-result time to approximately 30 min, rescue Limulus from being endangered and is resource efficient in terms of equipment and the non-use of a skilled technician.

Abstract Background Biological invasions threaten the functioning of ecosystems, biodiversity, and human well-being by degrading ecosystem services and eliciting massive economic costs. The European Union has historically been a hub for cultural development and global trade, and thus, has extensive opportunities for the introduction and spread of alien species. While reported costs of biological invasions to some member states have been recently assessed, ongoing knowledge gaps in taxonomic and spatio-temporal data suggest that these costs were considerably underestimated. Results We used the latest available cost data in InvaCost (v4.1)—the most comprehensive database on the costs of biological invasions—to assess the magnitude of this underestimation within the European Union via projections of current and future invasion costs. We used macroeconomic scaling and temporal modelling approaches to project available cost information over gaps in taxa, space, and time, thereby producing a more complete estimate for the European Union economy. We identified that only 259 out of 13,331 (~ 1%) known invasive alien species have reported costs in the European Union. Using a conservative subset of highly reliable, observed, country-level cost entries from 49 species (totalling US$4.7 billion; 2017 value), combined with the establishment data of alien species within European Union member states, we projected unreported cost data for all member states. Conclusions Our corrected estimate of observed costs was potentially 501% higher (US$28.0 billion) than currently recorded. Using future projections of current estimates, we also identified a substantial increase in costs and costly species (US$148.2 billion) by 2040. We urge that cost reporting be improved to clarify the economic impacts of greatest concern, concomitant with coordinated international action to prevent and mitigate the impacts of invasive alien species in the European Union and globally.

This study takes into account the multi-point thermal model's automatic adjustment mechanism. The combined heat balance test temperature is used as the genetic algorithm's optimization target value. To minimize time and resources when running simulations, a back propagation neural network-based surrogate model between temperature and parameters is used. The best parameter set is discovered when the thermal model is modified at high temperatures. The overall temperature distribution of the thermal model is significantly improved by the 50% decrease in the heat transfer coefficient X1 of the joint body and the multi-layer insulation assembly, among others. The model's root-mean-square error before and after the adjustment is decreased from 4.51 °C to 0.95 °C. The best parameter set has been effectively contrasted and validated by reintroducing it into the low temperature environment. When the model is updated, the root-mean-square error drops from 2.73 °C to 1.09 °C. Following the model update, the joint temperature distribution is more in accord with the experimental findings, and all errors are within 2 °C. Therefore, the finite element thermal model of a space manipulator joint can be altered using a combination of back propagation neural network and genetic algorithm.

BackgroundNeutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) play an important role in the inflammatory response in various diseases, but the role in the course of spontaneous intracerebral hemorrhage (ICH) is unclear.MethodsThis study retrospectively collected baseline characteristics and laboratory findings, including NLR and PLR at different time points, from spontaneous ICH patients undergoing surgery between January 2016 and June 2021. Patients were scored using the modified Rankin Scale (mRS) to evaluate their functional status at 30 days post-operation. Patients with mRS score ≥3 were defined as poor functional status, and mRS score

BackgroundNeurological involvement and psychiatric manifestations have been documented in clinical cases of inflammatory bowel disease (IBD); however, the presence of a causal relationship remains elusive. The objective of this study is to investigate the modifications occurring in the cerebral cortex as a result of IBD.MethodsA compendium of data extracted from a genome-wide association study (GWAS) involving a maximum of 133,380 European subjects. A series of Mendelian random analyses were applied to exclude heterogeneity and pleiotropy, ensuring the stability of the results.ResultsNeither IBDs nor inflammatory cytokines (IL-6/IL-6Rα) were found to have a significant causality with surface area (SA) and thickness (TH) at the global level. At the regional functional brain level, Crohn’s disease (CD) significantly decreased the TH of pars orbitalis (β=-0.003mm, Se=0.001mm, pivw =4.85×10-4). IL-6 was observed to reduce the SA of middle temporal (β=-28.575mm2, Se=6.482mm2, pivw=1.04×10-5) and increase the TH of fusiform (β=0.008mm, Se=0.002mm, pivw=8.86×10-5) and pars opercularis (β=0.009mm, Se=0.002mm, pivw=2.34×10-4). Furthermore, a causal relationship between IL-6Rα and an increase in the SA of superior frontal (β=21.132mm2, Se=5.806mm2, pivw=2.73×10-4) and the TH of supramarginal (β=0.003mm, Se=0.0002mm, pivw=7.86×10-37). All results passed sensitivity analysis and no heterogeneity and pleiotropy were detected.ConclusionThe correlation between IBD and changes in cerebral cortical structures implies the existence of a gut-brain axis at the organismal level. It is recommended that clinical patients with IBD prioritize long-term management of inflammation, as changes at the organismal level can lead to functional pathologies. Magnetic resonance imaging (MRI) may be considered as an additional screening option for IBD.

The objective of this study was to investigate the effect of waste milk with antibiotic residue on rumen fermentation and rumen bacterial composition of dairy calves during pre-weaned and post-weaned periods. A total of 24 Holstein male calves (43.4 ± 0.93 kg body weight, mean ± standard error) were allocated into four blocks based on birth date. Dairy calves were supplied 100% milk replacer (MR, n = 8), 50% milk replacer mixed with 50% waste milk (MM, n = 8), or 100% waste milk (WM, n = 8). Ruminal samples were collected at 49 and 63 days of age and then subjected to determinations of pH value, volatile fatty acids (VFA), ammonia nitrogen (NH3–N) and 16S rRNA gene amplicon sequencing. The results showed that feeding WM had no effect on the pH value, the concentrations of VFA (acetic acid, propionic acid, butyric acid, isovaleric acid, valeric acid), and NH3–N in dairy calves compared to feeding MR. However, from 49 to 63 days of age, the pH value (p

Invasive species have caused severe impacts on biodiversity and human society. Although the estimation of environmental impacts caused by invasive species has increased in recent years, economic losses associated with biological invasions are only sporadically estimated in space and time. In this study, we synthesized the losses incurred by invasions in Asia, based on the most comprehensive database of economic costs of invasive species worldwide, including 560 cost records for 88 invasive species in 22 countries. We also assessed the differences in economic costs across taxonomic groups, geographical regions and impacted sectors, and further identified the major gaps of current knowledge in Asia. Reported economic costs of biological invasions were estimated between 1965 and 2017, and reached a total of US$ 432.6 billion (2017 value), with dramatic increases in 2000–2002 and in 2004. The highest costs were recorded for terrestrial ectotherms, for species estimated in South Asia, and for species estimated at the country level, and were related to more than one impacted sector. Two taxonomic groups with the highest reported costs were insects and mammals, and two countries with the highest costs were India and China. Non-English data covered all of 12 taxonomic groups, whereas English data only covered six groups, highlighting the importance of considering data from non-English sources to have a more comprehensive estimation of economic costs associated with biological invasions. However, we found that the estimation of economic costs was lacking for most Asian countries and for more than 96% of introduced species in Asia. Further, the estimation is heavily biased towards insects and mammals and is very limited concerning expenditures on invasion management. To optimize the allocation of limited resources, there is an important need to better and more widely study the economic costs of invasive alien species. In this way, improved cost reporting and more collaborations between scientists and stakeholders are needed across Asia.

The impacts of invasive alien species are well-known and are categorised as a leading contributor to biodiversity loss globally. However, relatively little is known about the monetary costs incurred from invasions on national economies, hampering management responses. In this study, we used published data to describe the economic cost of invasions in Southeast Asia, with a focus on Singapore – a biodiversity-rich, tropical island city state with small size, high human density and high trade volume, three factors likely to increase invasions. In this country, as well as in others in Southeast Asia, cost data were scarce, with recorded costs available for only a small fraction of the species known to be invasive. Yet, the overall available economic costs to Singapore were estimated to be ~ US$ 1.72 billion in total since 1975 (after accounting for inflation), which is approximately one tenth of the total cost recorded in all of Southeast Asia (US$ 16.9 billion). These costs, in Singapore and Southeast Asia, were mostly linked to insects in the family Culicidae (principally Aedes spp.) and associated with damage, resource loss, healthcare and control-related spending. Projections for 11 additional species known to be invasive in Singapore, but with recorded costs only from abroad, amounted to an additional US$ 893.13 million, showing the potential huge gap between recorded and actual costs (cost records remain missing for over 90% of invasive species). No costs within the database for Singapore – or for other Southeast Asian countries – were exclusively associated with proactive management, highlighting that a shortage of reporting on the costs of invasions is mirrored by a lack of investment in management. Moreover, invasion cost entries in Singapore were under-reported relative to import levels, but total costs exceeded expectations, based on land area and population size, and to a greater extent than in other Southeast Asian countries. Therefore, the evaluation and reporting of economic costs of invasions need to be improved in this region to provide efficient data-based support for mitigation and management of their impacts.

Terrestrial ecosystems, owing to the presence of key socio-economic sectors such as agriculture and forestry, may be particularly economically affected by biological invasions. The present study uses a subset of the recently developed database of global economic costs of biological invasions (InvaCost) to quantify the monetary costs of biological invasions in Russia, the largest country in the world that spans two continents. From 2007 up to 2019, invasions costed the Russian economy at least US$ 51.52 billion (RUB 1.38 trillion, n = 94 cost entries), with the vast majority of these costs based on predictions or extrapolations (US$ 50.86 billion; n = 87) and, therefore, not empirically observed. Most cost entries exhibited low geographic resolution, being split between European and Asian parts of Russia (US$ 44.17 billion; n = 72). Just US$ 7.35 billion (n = 22) was attributed to the European part solely and none to the Asian part. Invasion costs were documented for 72 species and particularly insects (37 species). The empirically-observed costs, summing up to US$ 660 million (n = 7), were reported only for four species: two insects Agrilus planipennis Fairmaire and Cydalima perspectalis (Walker) and two plants Ambrosia artemisiifolia L. and Heracleum sosnowskyi Manden. The vast majority of economic costs were related to resource damages and economic losses, with very little reported expenditures on managing invasions in terrestrial ecosystems. In turn, agriculture (US$ 37.42 billion; n = 68) and forestry (US$ 14.0 billion; n = 20) were the most impacted sectors. Overall, we report burgeoning economic costs of invasions in Russia and identify major knowledge gaps, for example, concerning specific habitat types (i.e. aquatic) and management expenditures, as well as for numerous known invasive taxa with no reported economic costs (i.e. vertebrates). Given this massive, largely underestimated economic burden of invasions in Russia, our work is a call for improved reporting of costs nationally and internationally.

An experimental study is presented to measure the elastic, yielding, and crushing properties of individual particles under compression using substrates made of aluminum alloy, stainless steel, and sapphire. Carefully selected, highly spherical individual Ottawa sand particles of 0.75–1.1 mm in nominal diameter were compressed between two smooth substrates, and the load–deformation curves were analyzed by Hertz elastic contact theory to derive their reduced modulus and Young's modulus as well as yielding and crushing strengths, which vary significantly with the type of substrate materials. Further analysis of the yielding and plastic deformation at the particle-substrate contact shows that the yield strength or hardness of the substrate materials dominates the local contact behavior and hence affects the measured apparent yielding and crushing strengths. The two softer substrates (aluminum alloy and stainless steel) actually lead to underestimated apparent shear yield strengths of quartz particles by 60.4% and 54.2%, respectively, which are actually the yielding of substrates, while the true particle yielding occurs in the sapphire-particle contact. Moreover, the two softer substrates cause much overestimated crushing strengths of the quartz particles by 50.4% and 36.4%, respectively. Selection of inappropriate substrate materials and inappropriate interpretation of the particle-substrate contact can lead to significant errors in the measured yielding and crushing strengths. It is recommended that single particle compression testing uses substrates with yield strength greater than that of the tested particles and result interpretation also considers the elastic and yielding behaviors of the substrates.

Objective: This study investigated the acute effects of PNF stretching on hamstring flexibility and muscle stiffness of lower limbs between genders.Methods: 15 male and 15 female university students without any injury histories on lower limbs in the past 3 months were included in this study were selected. All subjects were measured by MyotonPRO before and after stretching to determine the muscle stiffness of the biceps femoris muscle (BF), semitendinosus muscle (ST) of the hamstring and the medial gastrocnemius muscles (MG), lateral gastrocnemius muscles (LG), and the soleus (SOL) of the triceps surae muscles. Additionally, their flexibility was measured using the sit-and-reach test (the SR test) and passive hip range of motion (ROM). Differences based on time (pre-stretching vs. post-stretching) and sex (females vs. males) were assessed using 2 × 2 repeated measures AVONA.Results: There was a significant decrease in the stiffness of the hamstring and triceps surae muscles after stretching (BF, MG, LG, and SOL: p < 0.001; ST: p = 0.003). The muscle stiffness of the hamstring and triceps surae muscles is larger in males than in females at all time points (p < 0.001). There was a significant increase in hip flexion angle and the SR test in males and females after PNF stretching (p < 0.001); However, there was no difference in the change in the muscle stiffness and the flexibility between genders (p > 0.05).Conclusion: PNF stretching helped improve hamstring flexibility and decrease muscle stiffness. Stretching the hamstrings can also contribute to a decrease in the stiffness of the triceps surae muscles. The muscle stiffness of males before and after stretching is always greater than that of females. However, there was no difference in the change of improvement in stretching between genders.

BackgroundDiabetes is a chronic metabolic disease characterized by hyperglycemia that often occurs in adults. Many studies have indicated that exercise is beneficial to the medical management of diabetes. Bibliometric analysis can help investigators to identify the current research concerns to guide future research directions. Nevertheless, the overview bibliometric analysis of this global research topic related to exercise and diabetes is lacking. The present bibliometric study aimed to investigate development trends and research hotspots of exercise and diabetes research and provide researchers with new perspectives in further studies.Materials and MethodsThe articles and reviews regarding exercise and diabetes between 2000 and 2020 were retrieved from the Web of Science Core Collection. The scientometrics analytical tool CiteSpace software was used to analyze the cooperation among countries/institutions/journals/authors, analysis of co-occurrence keywords, keywords bursts, and references.ResultsIn all, 3,029 peer-reviewed papers were found with a persistently increased tendency over time. The most prolific country and institution were the USA (965) and Univ Alberta (76), respectively. Diabetes Care published most papers (178) and was the most co-cited journal (2,630). Riddell MC had the most publications (53), and Sigal RJ was the most influential author (503 cited times). Colberg et al.'s paper (co-citation counts: 183) showed the strongest citation bursts by the end of 2020, which was the most representative reference. The four research focuses were mellitus, exercise, physical activity, and glycemic control. The two frontiers trends were sedentary behavior and stress. The combination of aerobic and resistance training can effectively improve glycemic control, decrease HbA1c levels, enhance cardiorespiratory fitness, improve lipid levels, and decrease the demand for non-insulin antihyperglycemic agents.ConclusionsThis study offers a scientific perspective on exercise and diabetes research and provides investigators with valuable information to detect the current research condition, hotspots, and emerging trends for further study.

Purpose: Monitoring the contractility of muscles assists the clinician in understanding how muscle functions as part of the kinetic system. This study investigated the effect of knee joint angles under different resistance on the stiffness of the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (SOL) muscles using the shear wave elastography (SWE) technique.Methods: A total of 22 females were recruited. During isometric plantar flexion, at knee 0-degree (fully extended) and knee 90-degree (flexed 90°), the shear modulus on the MG, LG, and SOL was measured by shear wave elastography at no contraction and two intensities (40% and 80%) of maximal voluntary contraction (MVC). Shear modulus is a mechanical parameter to describe stiffness, and stiffness is a proxy for muscle contractility.Results: There were moderate-to high-positive correlations between the active stiffness of triceps surae muscles and isometric contraction intensity (r: 0.57–0.91, p＜0.001). The active stiffness in MG and LG with extended knees was higher than that with flexed knees (p＜0.001). The active stiffness in SOL with flexed knee was higher than that with extended knee (p＜0.001).Conclusion: Active stiffness can be considered a quantitative indicator generated by the force output of the triceps surae. Different knee joint angles cause three triceps surae muscles to exhibit non-uniform mechanical properties, which may explain part of the mechanism of soft tissue injury during physical exercise.

Purposes: To detect the effects of hip joint position on the quadriceps recruitment pattern of different resistance levels of rectus femoris (RF), vastus intermedius (VI), vastus lateralis (VL), and vastus medialis obliquus (VMO) in healthy people during knee extension.Methods: Twenty healthy females performed isometric knee extension contractions at 0, 10, 20, and 30% of maximal voluntary isometric contraction (MVIC) with a 90° and 0° hip angle. Ultrasound shear-wave elastography was used to evaluate the shear elastic modulus of RF, VI, VL, and VMO during resting and contraction states.Results: At resting state, stiffness of RF was about 50% higher at 0° compared with at 90° of the hip (p < 0.01). There were significant differences in comparisons between 0 and 10% MVIC, 10 and 20% MVIC, and 20 and 30% MVIC in the four muscles, except that there was no significant difference between 20 and 30% MVIC for RF. There was a significant positive correlation between muscle stiffness and resistance level (r = 0.78–0.94, p < 0.001).Conclusions: Hip joint position had effects on the quadriceps recruitment pattern of different resistance levels in healthy people during knee extension.

Purpose: This study investigated the effects of isometric plantar-flexion against different resistances on the thoracolumbar fascia (TLF), erector spinae (ES), and gastrocnemius stiffness by shear wave elastography (SWE). The purpose was to explore the interaction between the lower limb muscle and lumbar tissue in the myofascial tensegrity network.Methods: Twenty healthy young female were recruited in this study. The stiffness of the TLF, ES, medial gastrocnemius (MG), and lateral gastrocnemius (LG) was measured by SWE under four isometric plantar-flexion resistance conditions. The resistance conditions involved 0% maximum voluntary isometric contraction (MVIC), 20% MVIC, 40% MVIC, and 60% MVIC.Results: There was a strong correlation between the stiffness change of MG and that of TLF (r = 0.768–0.943, p < 0.001) and ES (r = 0.743–0.930, p < 0.001), while it was moderate to strong correlation between MG and that of LG (r = 0.588–0.800, p < 0.001). There was no significant difference in the stiffness between the nondominant and dominant sides of TLF and ES under the resting position (p > 0.05). The increase in stiffness of the TLF, ES, MG, and LG, with MVIC percentage (p < 0.05), and the stiffness of TLF and ES on the nondominant side is much higher than that on the dominant side.Conclusions: Our data shows that isometric plantar-flexion has a significant effect on the stiffness of the lumbar soft tissue and gastrocnemius. The gastrocnemius has a strong correlation with the stiffness changes of TLF and ES, which provides preliminary evidence for exploring the myofascial tensegrity network between the dorsal side of the lower limb muscle and lumbar tissue.

Knee osteoarthritis is a disease with the degeneration of articular cartilage as its main feature. Cartilage thickness cannot become a single index to evaluate cartilage degeneration, so it is essential to also evaluate the stiffness. The purposes were as follows: (1) to examine test-retest reliabilities of the elastic modulus measurement in distal femoral articular cartilage (FAC) and compare the changes in specific-regional of distal FAC, (2) to explore the difference in distal FAC stiffness and thickness between the dominant and nondominant sides, and (3) to examine the correlation between the elastic properties of cartilage and the thickness of cartilage. Twenty healthy participants were recruited. The stiffness of distal FAC at the lateral femoral condyle (LFC), medial femoral condyle (MFC), and intercondylar notch (IN) was quantified using shear-wave elastography (SWE). Intra- and interrater reliabilities were excellent for measuring the stiffness of distal FAC (ICC: 0.83-0.98). About a 50% increase in the stiffness of LFC (40.78 kPa) was found when compared with IN (21.82 kPa) and MFC (18.34 kPa). No significant difference was found between the dominant and nondominant sides in distal FAC stiffness and thickness. There was no correlation between the stiffness and thickness of the distal FAC. In conclusion, SWE can quantify the stiffness of the distal FAC.

Background. The upper limb neurodynamic test 1 (ULNT1) consists of a series of movements that are thought to detect an increase in neuromechanical sensitivity. In vivo, no trail was made to quantify the association between the nerve elasticity and different limb postures during ULNT1. Objectives. (1) To investigate the relationship between nerve elasticity and limb postures during ULNT1 and (2) to investigate the intra- and interoperator reliabilities of shear wave elastography (SWE) in quantifying the elasticity of median nerve. Methods. Twenty healthy subjects (mean age: 19.9±1.4 years old) participated in this study. The median nerve was imaged during elbow extension in the following postures: (1) with neutral posture, (2) with wrist extension (WE), (3) with contralateral cervical flexion (CCF), and (4) with both WE and CCF. The intra- and interoperator reliabilities measured by two operators at NP and CCF+WE and intraclass correlation coefficients (ICCs) were calculated. Results. The intraoperator (ICC=0.72–0.75) and interoperator (ICC=0.89–0.94) reliabilities for measuring the elasticity of the median nerve ranged from good to excellent. The mean shear modulus of the median nerve increased by 53.68% from NP to WE+CCF. Conclusion. SWE is a reliable tool to quantify the elasticity of the median nerve. There was acute modulation in the elasticity of the median nerve during the ULNT1 when healthy participants reported substantial discomfort. Further studies need to focus on the elasticity properties of the median nerve in patients with peripheral neuropathic pain.

The thermal design of spacecraft becomes increasingly complicated as various advanced technologies are continuously introduced to the spacecraft. Determining and optimizing the uncertainties of a spacecraft thermal control system through global sensitivity analysis has long been an essential task for thermal engineers. It is a difficult task that relies heavily on engineering experience and is a time-intensive, trial-and-error endeavor that may not even lead to global optimization. Hence, an intelligent optimization strategy based on statistical machine learning for spacecraft thermal design, called IOSML, is proposed. An intelligent batch processing system (IBPS) based on MATLAB, Python, and NX/TMG real-time data interaction is designed. The IBPS uses a surrogate model to reduce the computational cost of global sensitivity analysis while using a detailed thermal mathematical model to maintain accuracy. We combine a Bayesian inference framework with a neural network surrogate spacecraft-thermophysical model that is 100× faster than numerical solvers. This article first reports on a density-based global sensitivity analysis that evaluates the effect of design parameters on the temperature difference between the complementary metal- oxide-semiconductor and cold screen of the Lehman Alpha Solar Space Telescope detector. From 42 design parameters, the most sensitive four are selected for optimization, and the temperature difference and the boundary temperature are used as the objective function. Adopting IOSML, under no supervision, four design parameters are optimized through the IBPS, and the effectiveness of the algorithm is verified by comparison with traditional methods. Additionally, IOSML is versatile and can be used in various complex engineering applications to provide guidance for the better selection of appropriate parameters and optimization.

In this work, we identified the seasonal and spatial variations in fish assemblages and their relation to environmental variables in the Yangtze Estuary and adjacent waters. A total of 61 fish species in 32 families were identified over four seasons; of these, 12 species were dominant and Harpadon nehereus was the most dominant species. Fish varied in abundance, biomass, and species composition in different seasons; both biomass and abundance were highest in autumn and lowest in spring. Fish can be spatially divided into high-salinity assemblages and low-salinity assemblages. The spatial variation in fish assemblages was caused by the selectivity and adaptability of species for the environmental conditions of the estuary and the seasonal variation in fish assemblage structure likely resulted from migrations of dominant taxa associated with fish spawning and foraging behavior as well as the environment. The results of CCA analysis showed that temperature, depth, salinity, dissolved oxygen, and chlorophyll were the major factors affecting the fish assemblage differences throughout the seasons. Of these, temperature drove the seasonal variation in assemblage structure, while salinity significantly affected the spatial distribution of assemblages. This paper revealed the relationship between the seasonal and spatial distribution patterns of the fish assemblage and environmental factors and the results could provide a scientific basis for the management and sustainable utilization of fishery resources in the Yangtze Estuary and adjacent waters.

Hyperlipidemia, a chronic disorder of abnormal lipid metabolism, can induce obesity, diabetes, and cardiovascular and cerebrovascular diseases such as coronary heart disease, atherosclerosis, and hypertension. Increasing evidence indicates that phytochemicals may serve as a promising strategy for the prevention and management of hyperlipidemia and its complications. At the same time, the concept of synergistic hypolipidemic and its application in the food industry is rapidly increasing as a practical approach to preserve and improve the health-promoting effects of functional ingredients. The current review focuses on the effects of single phytochemicals on hyperlipidemia and its mechanisms. Due to the complexity of the lipid metabolism regulatory network, the synergistic regulation of different metabolic pathways or targets may be more effective than single pathways or targets in the treatment of hyperlipidemia. This review summarizes for the first time the synergistic hypolipidemic effects of different combinations of phytochemicals such as combinations of the same category of phytochemicals and combinations of different categories of phytochemicals. In addition, based on the different metabolic pathways or targets involved in synergistic effects, the possible mechanisms of synergistic hypolipidemic effects of the phytochemical combination are illustrated in this review. Hence, this review provides clues to boost more phytochemical synergistic hypolipidemic research and provides a theoretical basis for the development of phytochemicals with synergistic effects on hyperlipidemia and its complications.

Biological invasions are a major threat to global biodiversity with particularly strong implications for island biodiversity. Much research has been dedicated towards understanding historic and current changes in alien species distribution and impacts on islands and potential changes under future climate change. However, projections of how alien species richness and impacts on islands might develop in the future are still lacking. In the absence of reliable projections, expert-based assessments are a valuable tool to investigate the importance of different drivers and pathways and the distributions of potential impacts of future biological invasions. These insights can guide subsequent quantification efforts and inform invasive species management and policy. In this study, we performed a survey among 126 experts in invasion science ranging from scientists to managers and decision makers with a focus on island systems until the mid-21st century. The survey revealed that out of 15 drivers, six were considered important by almost all respondents (>90%). Of these, trade and transport was identified as most important at the introduction stage (99.2%) and land use/cover change as most important at the establishment (96.8%) and spread (95.2%) stage. Additionally, the experts considered that alien species were more likely to be introduced (93.7%) and spread (78.6%) as stowaways than through any other pathway. In general, respondents agreed that the impacts of alien species will increase on all types of islands, particularly on oceanic islands, followed by atolls and continental islands. Within islands, terrestrial ecosystems were assumed to be impacted more severely than marine ecosystems. Finally, the survey hints toward the potential for effective communication, scientific research and increased pro-active management of alien species on islands to reduce their future consequences. Given the major threat represented by invasive alien species on islands, these results provide crucial insights relevant for global and regional conservation efforts.

Antimicrobial peptides (AMPs) have attracted more attention for their potential candidates for new antibiotic drugs. As a novel identified cathelicidin AMP from duck, dCATH owns broad-spectrum antimicrobial activities but with a noticeable toxicity. To explore dCATH-derived AMPs with reduced cell toxicity and improved cell selectivity, a series of truncated and tryptophan-replaced peptides of dCATH were designed. Two truncated peptides containing one of the two tryptophan (Trp) residues at the positions of 4 and 17 (W4 and W17) of dCATH, dCATH(1–16) and dCATH(5–20), showed strong antibacterial activity, but didn’t show obvious hemolytic activity and cytotoxicity. The derived peptides not containing Trp didn’t possess obvious antimicrobial activity, and their hemolytic and cytotoxic effect was also diminished. Also as evidence by Trp fluorescence experiment that existence of W4 and W17 was crucially important to the antimicrobial activity, hemolysis and cytotoxicity of dCATH, and one of the two Trp residues was competent and necessary to retain its antimicrobial activity. Antibacterial mechanism analysis showed that dCATH(1–16) and dCATH(5–20) killed bacterial cells by increasing permeability and causing a loss of membrane integrity. dCATH(1–16) and dCATH(5–20) possessed insignificant inhibitory activity against levels of IL-6, TNF-α, and NO in RAW 264.7 cells treated with LPS. In vivo, intraperitoneal administration of the two peptides significantly decreased mortality and provided protection against LPS-induced inflammation in mice challenged with lethal dose of LPS. The two peptides, dCATH(1–16) and dCATH(5–20), which possessed high antibacterial activity and cell selectivity, may herald development prospects as new antibacterial agents in the future.

Background. Neck and shoulder complaints caused by poor posture may influence upper trapezius stiffness. The relationship between the shear elastic modulus of the upper trapezius and cervical flexion angles is unknown. Therefore, it is essential to assess upper trapezius stiffness during cervical flexion. The objectives of this study were to (1) determine the intra- and interoperator reliabilities of evaluating upper trapezius stiffness and calculate the minimal detectable change (MDC); (2) examine the elastic modulus alterations of the upper trapezius during cervical flexion; and (3) explore the difference of upper trapezius stiffness between the dominant and nondominant sides. Methods. Twenty healthy male participants were recruited in this study. The shear modulus of the upper trapezius was evaluated by two independent investigators using shear wave elastography (SWE) during cervical flexion at 0° and 50°. Findings. The intraoperator (intraclass correlation coefficient ICC=0.85–0.86) and interoperator (ICC=0.94–0.98) reliabilities for measuring the shear elastic modulus of the upper trapezius during the cervical flexion ranged from good to excellent. An increase of 35.58% in upper trapezius stiffness was found at 0° to 50° of cervical flexion, and the MDC was 7.04 kPa. In addition, a significant difference was obtained in the elastic modulus of the upper trapezius muscle between the dominant and nondominant sides (P

Background. Anatomical studies and the mechanical property studies showed that there is a strong correlation between Achilles tendon (AT) elasticity and individual gastrocnemius muscle (the medial head of gastrocnemius (MG) and the lateral head of gastrocnemius (LG)) elasticity. Limited ankle dorsiflexion range of motion has been correlated with decreased flexibility of the MG/LG/AT complex. However, no studies have been conducted to examine the exact correlation between the Achilles tendon and the individual muscle of the gastrocnemius. Purposes. The purposes of the present study were (1) to evaluate intra- and interoperator reliabilities of elastic property measurements in the gastrocnemius muscle-Achilles tendon complex by using the shear wave elastography (SWE) and (2) to examine the correlation between the regional elastic properties of the AT and the individual muscle of the gastrocnemius. Methods. Twenty healthy subjects (mean age: 22.50 (3.02) years) were recruited in this study. The elastic properties of the AT and the individual muscle of the gastrocnemius were quantified using the SWE. Findings. The SWE has comparatively high reliability in quantifying the elastic properties of the muscle-tendon range from good to excellent. The intraoperator ICC of the gastrocnemius muscle-Achilles tendon complex was 0.77 to 0.95, while the interoperator ICC was 0.76 to 0.94. The minimal detectable change (MDC) of the muscle was 1.72 kPa, while the AT was 32.90 kPa. A significant correlation was found between the elastic modulus of AT and the elastic modulus of the MG (r=0.668 and p=0.001 at the relaxing position and r=0.481 and p=0.032 at the neutral position). Conclusions. The SWE has the potential to assess localized changes in muscle-tendon elastic properties, provide more intuitive relations between elastic properties of the muscle tendon and function, and evaluate the therapeutic effect of the muscle tendon. A significant correlation between the AT and the MG was found, and it may provide a new treatment idea (targeted to the tight muscle heads) for the clinical setting to treat subjects with AT disorders.

Resources of Japanese anchovy (Engraulis japonicus Temminck & Schlegel, 1846) are undergoing dramatic recessions in China as the consequence of intensifying anthropogenic activities. Elucidating the influences of local-scale environmental factors on early life history traits is of great importance to design strategies conserving and restoring the declining anchovy resources. In this research, we studied hatching date and early growth of anchovy in the Yangtze River Estuary (YRE) using information obtained from otolith microstructure. Onset of hatching season and growth rates of anchovy was compared to populations in Japan and Taiwan. In YRE, the hatching date of anchovy ranged from February 26th to April 6th and mean growth rate ranged from 0.27 to 0.77 mm/d. Anchovies hatching later had higher growth rates than individuals hatching earlier before the 25th day. Among populations, hatching onsets of anchovy from the higher latitude were later than populations in the lower latitude, and growth rates of anchovy in YRE were much lower than populations in Japan and Taiwan. Variations in hatching onsets and early growth patterns of anchovy thus provide important knowledge on understanding the adaptation of anchovy in YRE and designing management strategies on conserving China’s anchovy resources.