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Top-emitting organic light-emitting diodes (TEOLEDs) achieved an ultra-high power efficiency of up to 186.38 lm W−1, an impressive EQE as high as 47.4%, and a low operation voltage.

Proposing a simple strategy for developing full-color carbon quantum dots (CQDs) and exploring how the luminescence can be tuned and improved is attractive and encouraging. Herein, blue, green, yellow-green, and orange-red CQDs doped with heteroatoms were synthesized in one pot and separated by column chromatography, with emission peaks of 435 nm, 495 nm [photoluminescence quantum yield (PLQY) of 88.9%], 525 nm, and 595 nm (full width at half-maximum of 31 nm), respectively. The abundant C-O/C-O-C electron donor groups greatly improve the PLQY of green CQDs, and the expended effective conjugated domains (particle size, doped chlorine, and conjugated nitrogen) of CQDs boost the red-shifts of emission spectra. Energy transfer (ET) in a concentrated mixed solution of CQDs was discovered, and possible ET mechanisms are proposed. Furthermore, a high-efficiency white light-emitting diode with Commission Internationale de L'Eclairage coordinates of (0.361, 0.369), a correlated color temperature of 4534 K, and a high color rendering index of 90.8 was fabricated.

IntroductionAtrial fibrillation (AF) is common in patients with rheumatic mitral valve disease (RMVD) and increase the risk of stroke and death. Bi-atrial or left atrial ablation remains controversial for treatment of AF during mitral valve surgery. The study aims to compare the efficacy and safety of bi-atrial ablation with those of left atrial ablation among patients with RMVD and persistent or long-standing persistent AF.Methods and analysisThe ABLATION trial (Bi-atrial vs Left Atrial Ablation for Patients with RMVD and Non-paroxysmal AF) is a prospective, multicentre, randomised controlled study. The trial will randomly assign 320 patients with RMVD and persistent or long-standing persistent AF to bi-atrial ablation procedure or left atrial ablation procedure in a 1:1 randomisation. The primary end point is freedom from documented AF, atrial flutter or atrial tachycardia of >30 s at 12 months after surgery off antiarrhythmic drugs. Key secondary end point is the probability of freedom from permanent pacemaker implantation at 12 months after surgery. Secondary outcomes include the probability of freedom from any recurrence of atrial tachyarrhythmias with antiarrhythmic drugs, AF burden, incidence of adverse events and cardiac function documented by echocardiography at 12 months after operation.Ethics and disseminationThe central ethics committee at Fuwai Hospital approved the ABLATION trial. The results of this study will be disseminated through publications in peer-reviewed journals and conference presentations.Trial registration numberNCT05021601.

The necessity of complete bi-atrial lesion created by radiofrequency clamp and pen for nonparoxysmal atrial fibrillation (AF) in patients with rheumatic mitral valve disease (RMVD) remains unclear.From January 2014 to December 2018, patients with RMVD concomitant with nonparoxysmal AF who underwent mitral valve surgery concomitant surgical ablation were retrospectively enrolled. We divided patients into Group A (complete bi-atrial lesion set created by radiofrequency clamp and pen) and Group B (simplified lesion sets created by radiofrequency clamp alone including bi-atrial ablation with incomplete mitral isthmus line and stand-alone left atrial ablation) according to the surgical ablation lesion sets. Propensity score matching was applied to analyze freedom from atrial tachyarrhythmias between the two groups.Two hundred eight (38.5%) and 332 (61.5%) patients were divided into Group A and Group B, respectively. In Group B, the proportion of patients with recurrent atrial flutter in the subgroup of bi-atrial ablation with incomplete mitral isthmus line was higher than that in Group A (p = .044). After propensity score matching, there were 203 patients in each group. Better freedom from atrial tachyarrhythmias without antiarrhythmic drugs was obtained in Group A (83.1%, 79.6%, and 65.4%) than Group B (73.1%, 68.4%, and 52.7%) at 12, 36, and 60 months after operation (p = .012).The application of radiofrequency clamp and pen to create complete bi-atrial lesion set in surgical ablation for nonparoxysmal AF in RMVD was associated with superior long-term efficacy.

OBJECTIVESThe short-term results of video-assisted thoracoscopic surgical ablation (VTSA) with box lesion have been highly variable, and the actual efficacy requires clarification through longer follow-ups. We aimed to report which patients might benefit more from VTSA with box lesion by longer follow-up.METHODSFrom September 2010 to November 2016, patients with atrial fibrillation (AF) who underwent VTSA with box lesion were screened. All enrolled patients visited the outpatient clinic and underwent 24-h Holter monitoring at 3, 6 and 12 months after surgery and annually thereafter.RESULTSA total of 91 consecutive patients [age, 58.0 (interquartile interval from 52.0 to 62.0) years; male, 71.4%] with paroxysmal (67%) or persistent/long-standing persistent (33%) AF were enrolled. After a median follow-up of 48 months (interquartile interval from 36 to 60 months), freedom from atrial tachyarrhythmias without antiarrhythmic drugs (AADs) was 76.5%, 66.0% and 66.0% for paroxysmal AF and 58.6%, 47.8% and 34.2% for persistent/long-standing persistent AF at 12, 36 and 60 months, respectively (P = 0.017). The preoperative left atrial diameter >40 mm (hazard ratio: 2.837, 95% confidence interval: 1.408–5.716; P = 0.004) and age >50 years (hazard ratio: 2.927, 95% confidence interval: 1.359–6.305; P = 0.006) were associated with recurrences of atrial tachyarrhythmias. In patients with paroxysmal AF and left atrial diameter ≤40 mm (n = 43), freedom from atrial tachyarrhythmias without AADs was 81.4%, 74.3% and 74.3% at 12, 36 and 60 months, respectively.CONCLUSIONSIn patients with paroxysmal AF and left atrial diameter ≤40 mm, 5-year freedom from atrial tachyarrhythmias without AADs was 74.3%, which was better than that in patients with left atrial diameter >40 mm. A larger sample size and improved study design are needed to confirm our conclusions.

Protein self-assembly is one of the formation mechanisms of biomolecular condensates. However, most phase-separating systems (PS) demand multiple partners in biological conditions. In this study, we divided PS proteins into two groups according to the mechanism by which they undergo PS: PS-Self proteins can self-assemble spontaneously to form droplets, while PS-Part proteins interact with partners to undergo PS. Analysis of the amino acid composition revealed differences in the sequence pattern between the two protein groups. Existing PS predictors, when evaluated on two test protein sets, preferentially predicted self-assembling proteins. Thus, a comprehensive predictor is required. Herein, we propose that properties other than sequence composition can provide crucial information in screening PS proteins. By incorporating phosphorylation frequencies and immunofluorescence image-based droplet-forming propensity with other PS-related features, we built two independent machine-learning models to separately predict the two protein categories. Results of independent testing suggested the superiority of integrating multimodal features. We performed experimental verification on the top-scored proteins DHX9, K i -67, and NIFK. Their PS behavior in vitro revealed the effectiveness of our models in PS prediction. Further validation on the proteome of membraneless organelles confirmed the ability of our models to identify PS-Part proteins. We implemented a web server named PhaSePred ( http://predict.phasep.pro/ ) that incorporates our two models together with representative PS predictors. PhaSePred displays proteome-level quantiles of different features, thus profiling PS propensity and providing crucial information for identification of candidate proteins.

Phase separation is an important mechanism that mediates the compartmentalization of proteins in cells. Proteins that can undergo phase separation in cells share certain typical sequence features, like intrinsically disordered regions (IDRs) and multiple modular domains. Sequence-based analysis tools are commonly used in the screening of these proteins. However, current phase separation predictors are mostly designed for IDR-containing proteins, thus inevitably overlook the phase-separating proteins with relatively low IDR content. Features other than amino acid sequence could provide crucial information for identifying possible phase-separating proteins: protein-protein interaction (PPI) networks show multivalent interactions that underlie phase separation process; post-translational modifications (PTMs) are crucial in the regulation of phase separation behavior; spherical structures revealed in immunofluorescence (IF)images indicate condensed droplets formed by phase-separating proteins, distinguishing these proteins from non-phase-separating proteins. Here, we summarize the sequence-based tools for predicting phase-separating proteins and highlight the importance of incorporating PPIs, PTMs, and IF images into phase separation prediction in future studies.

Stroke is the most common, deadly, and complicating neurological disease. Many studies have shown that the levels of some acute inflammatory reactants in people with ischemic stroke are higher than average. Therefore, in this study, three acute inflammatory reactants, i.e., C-reactive protein, Serum cystatin C, and carbohydrate antigen 125, were evaluated in patients with acute ischemic stroke to consider the association between these serums with intra and extra-cerebral vessels stenosis. In this cross-sectional study, 90 patients with non-embolic ischemic stroke were evaluated. The diagnosis was by physical examination, rejection of emboli, and brain imaging. Blood samples were taken in the first 24 hours of a stroke. ELISA test was used to measure CRP, Serum cystatin C, and CA125. Doppler ultrasound of cerebral arteries was also performed in the first five days. Independent chi-square and t-tests were used to analyze the data. The result of CRP level in patients with stenosis was 7.58±1.33μg/ml and in patients without stenosis was 4.10±1.75μg/ml (p = 0.004). Also, there was a significant relationship between serum CRP level and stenosis (p = 0.003). In patients with abnormal CRP, the internal carotid artery, middle cerebral artery, and anterior cerebral artery were the most involved. In patients with normal CRP, the most involved arteries were the anterior cerebral artery, internal carotid artery, and middle cerebral artery, respectively. There was a significant relationship between serum CRP level and the location of internal carotid artery stenosis (p = 0.015) and middle cerebral artery (p = 0.006). The amount of cystatin C between the normal CRP and abnormal CRP groups was statistically significant so that its concentration in the normal group was less than in the abnormal group (p = 0.04). The results of measuring the serum concentration of carbohydrate antigen 125 showed that the serum level in the normal group was statistically lower than in the abnormal group (P = 0.02). The results showed that stenosis of the internal carotid artery and middle cerebral artery is more common in patients with ischemic stroke with high serum CRP levels. This finding suggests that abnormal CRP may be more associated with narrowing some cerebral arteries.

Amines, including amino acids and biogenic amines are widely recognized as cancer biomarkers for early diagnosis and treatment. But rapid analysis of amines in biological samples with high sensitivity, good durability and low cost is still challenging. Herein, we proposed an ultrafast biological sample preparation method based on in-fiber extraction and derivatization using a homemade needle-tip devise. Under the nanoconfinement effect, the extraction was completed within 2 min in nanofibers, followed by the in-suit derivatization within 3 min under room temperature using pentafluoropropionic anhydride (PFA) as derivatization reagent, then the needle was inserted into the inlet of GC-MS directly for thermal desorption to enable the complete injection. Additionally, the established sample preparation strategy consumed only 20 μL organic solvent and urine sample, and the sensitivity was increased compared with other methods. After systematical optimization and validation, coupled to GC-MS, the established method was successfully applied to quantify twenty amines in urine of healthy people and lung, colorectal and breast cancer patients. The diagnosis biomarkers for each cancer were screened out and accessed by multiple statistical approaches. Then we further constructed the cancer diagnosis model of each cancer through binary logistic regression analysis for clinical use. Taken together, the established nanoconfinement effect based in-fiber extraction and derivatization biological sample preparation strategy in combination with GC-MS was an ultrafast method for biological analysis with high sensitivity, good durability, little organic solvent consumption and low cost. It possesses great potential in clinical cancer screening and treatment.

We propose a simple germanium Schottky photodiode, in which an ultrathin Al2O3 or HfO2 interlayer is inserted between the indium-doped tin oxide (ITO) and n-Ge contact, showing low dark current and ultra-high responsivity (gain). The introduction of 2 nm thick Al2O3 interlayer in a ITO/n-Ge Schottky photodetector results in 138 × reduction of dark current, and 16 × improvement of responsivity, exhibiting a low dark current density of 32 mA/cm2 and high responsivity of 12.5 A/W (external quantum efficiency: 1183%) for 1310 nm and 7.3 A/W (external quantum efficiency: 584%) for 1550 nm at − 4 V reverse bias, respectively. The large responsivity up to 18.5 A/W is obtained for 1310 nm at − 9 V for ITO/Al2O3(2 nm)/n-Ge diode, which corresponds to a large photoconductive gain of 24. Such a high photoconductive gain may be attributed to the capture of holes by the traps at the interface. These results are of significance for the fabrication of Ge photodetector with high performances.

In this paper, a kind of exhaust treatment device for drilling diesel engine with simple structure, low resistance, high filtration efficiency, wide collecting particle size range and low energy consumption was designed. Using RNG $$k - \varepsilon$$ turbulence model, the gas-phase flow field of the tail gas treatment unit and the gas-phase flow field of the conventional cyclone separator without corona pole were numerically calculated, and the three-dimensional velocity distribution law of the flow field in the tail gas treatment unit was studied. Compared with the conventional cyclone separator, the results show that the tangential velocity disturbance of the inner flow field is more obvious, the mean tangential velocity decreases, and the velocity in the outer vortex area decreases by 20%. Meanwhile, due to the influence of corona electrode, the eddy current flow in the treatment device is more disordered. The inner axial velocity of the processing device is slower in the down-flow area and larger in the up-flow area. Relatively speaking, the radial velocity magnitude is small, and the distribution is non-axisymmetric.

Targeted metabolomics with absolute quantification is widely applied in biomarker identification and drug screening. However, due to the complexity of biological matrix and trace amount of metabolites in plasma, simultaneous quantification of highly polar metabolites in plasma with broad coverage in short time is still challenging. Herein, we proposed a nanoconfined liquid phase nanoextraction (NLPNE) combined with in-fiber derivatization (IFD) strategy that enabled simultaneous quantification of seventy amino-containing analytes in plasma, including amines, nucleosides and their metabolites. Methanol-water (2:1, v/v) was selected as nanoconfined solvent (NCS) to quickly extract highly polar analytes based on the nanoconfinement effect, followed by IFD process directly performed by adding the derivatization reagent benzoyl chloride (BzCl) within 5 min. Besides saving time, this combination strategy was environment-friendly with little organic solvent consumption and cost-effective by using reusable carbon nanofibers. Furthermore, the sensitivity was increased up to 4.92-fold compared with protein precipitation (PP) based conventional derivatization method. Key factors that affected derivatization efficiency including the derivatization time, the amount of derivatization reagent, desorption solution and CNFs, were optimized by response surface methodology (RSM). After systematical method validation, this methodology was applied to determine the multi-metabolites index in plasma of lung cancer using an integrated data processing workflow. Then lung cancer diagnosis model was established through binary logistic regression analysis to make a reference for quick lung cancer screening clinically. Taken together, the NLPNE-IFD LC-MS/MS method for targeted metabolomics enables simultaneous quantification of seventy amino-containing analytes with advantages of broad coverage, high sensitivity, time- and solvent-saving, which could be used on cancer diagnosis clinically.

Under the normal conditions of the new economy, people’s living standards have improved; the amount of urban domestic waste has increased suddenly; and the differentiation of waste has become more and more complicated. However, the garbage transportation method at this stage has been difficult to meet the needs of reality. Therefore, it is particularly urgent to reform the original and simple treatment methods and adopt the latest and more reasonable garbage transportation. The urban waste transportation route optimization described in this article is quite complicated in the specific problems faced in the optimization of the urban domestic waste transportation route. This paper proposes to combine computer science to study the system optimization model and combine information management and other disciplines to develop and apply research on the dynamic management software of urban waste logistics systems. In the modern service industry, logistics occupies an important position. The optimization of urban waste transportation routes based on genetic algorithms also promotes the development of high-end modern service industries and is an important task to promote urban development and maintained hygiene and improve the healthcare of the citizens. The optimization of urban waste transportation routes is conducive to creating a clean and tidy urban environment.

Summary The diffuse-type gastric cancer (DGC) constitutes a subgroup of gastric cancer with poor prognosis and no effective molecular therapies. Here, we report a phosphoproteomic landscape of DGC derived from 83 tumors together with their nearby tissues. Based on phosphorylation, DGC could be classified into three molecular subtypes with distinct overall survival (OS) and chemosensitivity. We identified 16 kinases whose activities were associated with poor OS. These activated kinases covered several cancer hallmark pathways, with the MTOR signaling network being the most frequently activated. We proposed a patient-specific strategy based on the hierarchy of clinically actionable kinases for prioritization of kinases for further clinical evaluation. Our global data analysis indicates that in addition to finding activated kinase pathways in DGC, large-scale phosphoproteomics could be used to classify DGCs into subtypes that are associated with distinct clinical outcomes as well as nomination of kinase targets that may be inhibited for cancer treatments., Graphical Abstract, Highlights • A phosphoproteomic landscape of diffuse-type gastric cancer (DGC) was depicted • DGC could be classified into three subtypes based on phosphorylation data • A bioinformatics workflow was used to identify 16 kinases as potential drug targets • A patient-specific strategy for nomination of kinases was proposed, Biological Sciences; Cancer Systems Biology; Proteomics; Systems Biology

It's widely appreciated that liquid–liquid phase separation (LLPS) underlies the formation of membraneless organelles, which function to concentrate proteins and nucleic acids. In the past few decades, major efforts have been devoted to identify the phase separation associated proteins and elucidate their functions. To better utilize the knowledge dispersed in published literature, we developed PhaSepDB (http://db.phasep.pro/), a manually curated database of phase separation associated proteins. Currently, PhaSepDB includes 2914 non-redundant proteins localized in different organelles curated from published literature and database. PhaSepDB provides protein summary, publication reference and sequence features of phase separation associated proteins. The sequence features which reflect the LLPS behavior are also available for other human protein candidates. The online database provides a convenient interface for the research community to easily browse, search and download phase separation associated proteins. As a centralized resource, we believe PhaSepDB will facilitate the future study of phase separation.

Strain evolution in SiGe-on-insulator fabricated by a modified germanium condensation technique was studied. As enrichment of Ge content, the condensation temperature was proposed to decrease from 1150 to 900 °C through five steps, rather than only two different temperatures (1150 °C and 900 °C) were used as reported previously. Compared to condensation recipe with only two different temperatures, the modified condensation recipe was beneficial to obtain more uniform SiGe layers with lower surface roughness, thus better material quality. As Ge content enriched to 0.54, the strain in SiGe is almost fully relaxed with surface roughness RMS of less than 0.59 nm at condensation temperature of ≥1050 °C. After further condensation at 900 °C, the strain in SiGe accumulated dramatically to −1.23% with a surface roughness RMS of 0.66 nm. At the final stage of Ge condensation process, most of the compressive strain was sustained in Ge-rich SiGe layer. However, when pure GOI was obtained, the compressive strain could be almost fully relaxed by intensive over-oxidation without surface deterioration. The fully relaxed SiGe-on-insulator and germanium-on-insulator with low surface roughness could be a good template for secondary growth of strained Si and Ge, respectively.

Single crystalline bismuth nanowire is recently considered as one of the most attractive low dimensional materials for the exploration of exotic higher-order topological properties. However, its growth mechanism by sputtering, which is regarded as one of the most cost-effective and simplest method, is still unrevealed. In this work, a bismuth nanowire growth model based on surface diffusion driven by chemical potential difference among crystal facets is proposed for sputtering method. The morphology evolution of bismuth nanowires is captured for the first time, and three corresponding growth stages are clearly discriminated. The possible self-catalyzed, stress-driven, and screw dislocation-driven nanowire growth mechanisms are precluded separately based on the theoretical and experimental data. The thoroughly understanding of growth mechanism is fundamental and necessary for fabrication of size-controllable bismuth nanowires and may pave the way for the preparation of other low dimensional materials and the investigation of novel physics.

The defect of pipe dent has a great threat to the safety of oil and gas pipe transportation. In this paper, the numerical calculation model of pipe extruded by cylindrical indenter at vertical and transverse direction was established. Effects of indenter displacement, indenter radius and internal pressure on the dent behavior were discussed. The results show that different action forms of the indenter would cause different dent behaviors. A large degree of plastic deformation is formed at the edge of the cylinder end of the indenter when indenter made a vertical action. The larger the indenter radius is, the larger the plastic deformation is. But when the indenter extrudes transversely, the change in the indenter radius has little influence. For pressure pipeline, the strain in the dent fluctuates in a certain degree, and the plastic strain is larger than that in the non-pressure pipeline, but the dent depth would be lower in the end. Under different conditions of internal pressure, the laws of dent with “non-pressure” and “loading pressure” are the same, and the dented laws of “pressure” and “unloading pressure” are consistent. The strain and the depth of the dent achieve an extreme point or maximum near the edge of the dent.

N6-Methyladenosine (m6A) is the most prevalent internal modification that occurs in the mRNA of eukaryotes and plays a vital role in the post-transcriptional regulation. Recent studies highlighted the biological significance of m6A modification in the nervous system, and its dysregulation has been shown to be related to degenerative and neurodevelopmental diseases. Parkinson's disease (PD) is a common age-related neurological disorder with its pathogenesis still not fully elucidated. Reports have shown that epigenetic mechanisms including DNA methylation and histone acetylation, which alter gene expression, are associated with PD. In this study, we found that global m6A modification of mRNAs is down-regulated in 6-OHDA-induced PC12 cells and the striatum of PD rat brain. To further explore the relationship between m6A mRNA methylation and molecular mechanism of PD, we decreased m6A in dopaminergic cells by overexpressing a nucleic acid demethylase, FTO, or by m6A inhibitor. The results showed that m6A reduction could induce the expression of N-methyl-d-aspartate (NMDA) receptor 1, and elevate oxidative stress and Ca2+ influx, resulting in dopaminergic neuron apoptosis. Collectively, m6A modification may play a vital role in the death of dopaminergic neuron, which provides a novel view of mRNA methylation to understand the epigenetic regulation of Parkinson's disease.

Background Molecular subtyping of cancer aimed to predict patient overall survival (OS) and nominate drug targets for patient treatments is central to precision oncology. Owing to the rapid development of phosphoproteomics, we can now measure thousands of phosphoproteins in human cancer tissues. However, limited studies report how to analyse the complex phosphoproteomic data for cancer subtyping and to nominate druggable kinase candidates. Findings In this work, we reanalysed the phosphoproteomic data of high-grade serous ovarian cancer (HGSOC) from the Clinical Proteomic Tumour Analysis Consortium (CPTAC). Our analysis classified HGSOC into 5 major subtypes that were associated with different OS and appeared to be more accurate than that achieved with protein profiling. We provided a workflow to identify 29 kinases whose increased activities in tumours are associated with poor survival. The altered kinase signalling landscape of HGSOC included the PI3K/AKT/mTOR, cell cycle and MAP kinase signalling pathways. We also developed a “patient-specific” hierarchy of clinically actionable kinases and selected kinase inhibitors by considering kinase activation and kinase inhibitor selectivity. Interpretation Our study offered a global phosphoproteomics data analysis workflow to aid in cancer molecular subtyping, determining phosphorylation-based cancer hallmarks and facilitating nomination of kinase inhibition in cancer.

OBJECTIVES Thoracoscopic epicardial ablation with a limited lesion set led to suboptimal results for advanced paroxysmal atrial fibrillation (AF) or persistent AF. Whether additional right atrial lesions improve the result is unclear. METHODS We conducted a retrospective study involving 80 consecutive patients with paroxysmal or persistent AF, left atrial (LA) dilation (LA diameter >40 mm) and failed prior interventional ablation (40 patients, 50%) who underwent thoracoscopic epicardial ablation with box lesions (36 patients) or bi-atrial (BA) lesion (44 patients) in our institution. Freedom from atrial tachyarrhythmias after the procedures was compared between the box lesion group and BA lesion group. RESULTS Baseline differences included more patients with persistent AF (86.4% vs 47.2%) and larger left atrium [48.00 (44.00–50.75) vs 42.00 (41.25–44.00) mm] in the BA lesion group. There was no difference in procedural complications between the 2 groups. After a mean follow-up of 32 months, the freedom from atrial tachyarrhythmias off antiarrhythmic drugs at 6, 12 and 24 months was 77.2%, 77.2% and 77.2% in the BA lesion group and 69.4%, 50.0% and 40.6% in the box lesion group, respectively (P = 0.006). After adjustment for sex, age, body mass index, LA diameter, AF type, history of AF, and previous interventional ablation, BA lesion was an independent predictor of lower atrial tachyarrhythmia recurrence (hazard ratio 0.447, 95% confidential interval 0.208–0.963; P = 0.040). CONCLUSIONS Compared with the box lesion set, thoracoscopic epicardial ablation with BA lesion sets might provide better freedom from atrial tachyarrhythmias for paroxysmal or persistent AF with LA dilation. Randomized control trials are warranted to confirm the benefit of BA lesion sets in these patients.

Background: Postoperative atrial fibrillation (POAF) is the most common complication of cardiac surgery, which increases the risk of stroke and death, the length of hospital stays, and costs. Guide...

Corrosion and erosion are the main factors of pipes failure. Erosion wear characteristics of elbow pipe with corrosion defect are investigated in this paper. Initially, the erosion result of nondefect elbow pipe is calculated and compared to elbow pipe with corrosion defect. An elliptical erosion area with a V-shaped dent occurs at the extrados due to the particles' impact. The uniform corrosion defects change the structure of the pipe's inner surface. Therefore, the defect areas are more prone to be impacted by particles and then more severe erosion occurs. The maximum erosion rates of corrosion defect and noncorrosion area synchronously reach the maximum value as the corrosion defect located at 55 deg. The larger the particle mass flow rate is, the more severe the elbow pipe erosion is. As the flow rate increases, erosion rates of corrosion area and noncorrosion area increase, while the maximum erosion rate of corrosion area occurs at 55 deg. Structural parameters of corrosion defects affect the erosion. As the distance between two corrosion defects increases, the maximum erosion rate of lower corrosion defect increases. Considering the location, structure, and number of corrosion defect, a larger corrosion defect located in the middle of extrados has a worse effect on elbow pipe safety.

LC-MS-based metabolomics offers the potential of discovering biomarkers and exploring the mechanisms of underlying diseases. However, given the enormous polarity difference between metabolites, simultaneous across-polarity quantification for broad metabolome coverage has still been challenged by limited sample preparation methods and other hurdles. Herein, we proposed a consecutive extraction strategy based on nanoconfined liquid phase nanoextraction (NLPNE) technique. By modulating the nanoconfined solvents and coupling with LC-MS/MS, this method could simultaneously quantify metabolites with different polarities assigned to three classes, including amines (high polarity), steroids (middle polarity) and lysophosphatidylcholines (LPCs, low polarity) with high selectivity and high efficiency. During the systematical optimization of the extraction workflow, response surface methodology (RSM) was used for key parameters optimization. And consecutive extraction mode and parallel extraction mode were proposed in the choice of integrated extraction strategy. Then the consecutive NLPNE method was compared with two conventional sample preparation methods in metabolomics, protein precipitation (PP) and liquid-liquid extraction (LLE). After systematical validation, the consecutive NLPNE method coupled with LC-MS/MS was successfully applied in the identification of multi-metabolites indexes for lung, colorectal, and gastric cancer plasma samples from healthy controls, and among different types of cancer with student's t-test, partial least squares discriminant analysis (PLS-DA) and logistic regression-receiver operating characteristic (ROC) curve analysis. Taken together, the developed methodology is a versatile candidate in metabolomics for high coverage detection and may be used as a powerful tool for cancer diagnosis.

Amine/phenol submetabolome has shown critical role in clinical cancer screening and therapy. In suit derivatization has widely applied in the analysis of amine/phenol submetabolome by LC/MS for simplifying the pretreatment procedures, improving the separation and sensitivity. However, the complexity of biological matrix and trace amount of metabolites in plasma that lead to the limited detection coverage, poor repeatability and low extraction efficiency are still issues for in suit derivatization. Herein, we proposed an isotope labelled in suit derivatization-extraction integrated system for targeted analysis of all the metabolites in amine/phenol submetabolome with high efficiency and repeatability by LC-MS. The processes of in suit derivatization, alkalization and extraction were performed simultaneously in the nanopores highly dispersed between the carbon nanofibers based on the nanoconfinement effect. Isotope labelling derivatization (ILD) reagents benzoyl chloride (BzCl) and BzCl-d

Phase separation is an important mechanism that mediates the spatial distribution of proteins in different cellular compartments. While phase-separated proteins share certain sequence characteristics, including intrinsically disordered regions (IDRs) and prion-like domains, such characteristics are insufficient for making accurate predictions; thus, a proteome-wide understanding of phase separation is currently lacking. Here, we define phase-separated proteomes based on the systematic analysis of immunofluorescence images of 12 073 proteins in the Human Protein Atlas. The analysis of these proteins reveals that phase-separated candidate proteins exhibit higher IDR contents, higher mean net charge and lower hydropathy and prefer to bind to RNA. Kinases and transcription factors are also enriched among these candidate proteins. Strikingly, both phase-separated kinases and phase-separated transcription factors display significantly reduced substrate specificity. Our work provides the first global view of the phase-separated proteome and suggests that the spatial proximity resulting from phase separation reduces the requirement for motif specificity and expands the repertoire of substrates. The source code and data are available at https://github.com/cheneyyu/deepphase.