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138 results on '"Chupin, S."'

1. Behaviour of oscillations in loop structures above active regions

Author

Kolobov, D. Y., Kobanov, N. I., Chelpanov, A. A., Kochanov, A. A., Anfinogentov, S. A., Chupin, S. A., Myshyakov, I. I., and Tomin, V. E.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

In this study we combine the multiwavelength ultraviolet -- optical (Solar Dynamics Observatory, SDO) and radio (Nobeyama Radioheliograph, NoRH) observations to get further insight into space-frequency distribution of oscillations at different atmospheric levels of the Sun. We processed the observational data on NOAA 11711 active region and found oscillations propagating from the photospheric level through the transition region upward into the corona. The power maps of low-frequency (1--2 mHz) oscillations reproduce well the fan-like coronal structures visible in the Fe ix 171A line. High frequency oscillations (5--7 mHz) propagate along the vertical magnetic field lines and concentrate inside small-scale elements in the umbra and at the umbra-penumbra boundary. We investigated the dependence of the dominant oscillation frequency upon the distance from the sunspot barycentre to estimate inclination of magnetic tubes in higher levels of sunspots where it cannot be measured directly, and found that this angle is close to 40 degrees above the umbra boundaries in the transition region.

Published
2015
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3. Height distribution of the power of 3-min oscillations over sunspots

Author

Kobanov, N. I., Kolobov, D. Y., Chupin, S. A., and Nakariakov, V. M.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

Context. The height structure of 3-min oscillations over sunspots is studied in the context of the recently discovered effect of height inversion: over the umbra, the spatial location of the maximum of chromospheric 3-min oscillation power corresponds to the relative decrease in the power of photospheric oscillations. Aims. We investigate whether the height inversion of the power of 3-min oscillations is a common feature of the spatial structure of the oscillations for the majority of sunspots. Methods. Spectrogram sequences of H-alpha 6563{\AA} and FeI 6569{\AA} over sunspots, acquired with very high cadency (about 2 s or better) are obtained. The distribution of the oscillation power of the line-of-sight velocity signal is studied by using methods of wavelet frequency filtration and Fourier analysis. Results. The effect of the height inversion is found in 9 of 11 analyzed active regions. The interpretation of this effect is possibly connected to both the decrease in the level of photosphere in sunspot umbrae and the magnetic field topology.

Published
2011
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4. Oscillations in the Solar Faculae. III. The Phase Relations between Chromospheric and Photospheric LOS Velocities

Author

Kobanov, N. I., Kustov, A. S., Chupin, S. A., and Pulyaev, V. A.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

An analysis of line-of-sight velocity oscillation in nine solar faculae was undertaken with the aim of studying of phase relations between chromosphere (He i 10830 A line) and photosphere (Si i 10827 A line) five-minute oscillations. We found that time lag of the chromospheric signal relative to photospheric one varies from -12 to 100 seconds and is about 50 seconds on average.We assume that the small observed lag can have three possible explanations: i) convergence of formation levels of He i 10830 A and Si i 10827 A in faculae; ii) significant increase of five-minute oscillation propagation velocity above faculae; iii) simultaneous presence of standing and travelling waves., Comment: 12 pages, 6 figures, accepted in Solar Physics

Published
2011
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5. Multilevel Analysis of Oscillation Motions in Active Regions of the Sun

Author

Abramov-Maximov, V. E., Gelfreikh, G. B., Kobanov, N. I., Shibasaki, K., and Chupin, S. A.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

We present a new method that combines the results of an oscillation study made in optical and radio observations. The optical spectral measurements in photospheric and chromospheric lines of the line-of-sight velocity were carried out at the Sayan Solar Observatory. The radio maps of the Sun were obtained with the Nobeyama Radioheliograph at 1.76 cm. Radio sources associated with the sunspots were analyzed to study the oscillation processes in the chromosphere-corona transition region in the layer with magnetic field B=2000 G. A high level of instability of the oscillations in the optical and radio data was found. We used a wavelet analysis for the spectra. The best similarities of the spectra of oscillations obtained by the two methods were detected in the three-minute oscillations inside the sunspot umbra for the dates when the active regions were situated near the center of the solar disk. A comparison of the wavelet spectra for optical and radio observations showed a time delay of about 50 seconds of the radio results with respect to optical ones. This implies a MHD wave traveling upward inside the umbral magnetic tube of the sunspot. Besides three-minute and five-minute ones, oscillations with longer periods (8 and 15 minutes) were detected in optical and radio records., Comment: 17 pages, 9 figures, accepted to Solar Physics (18 Jan 2011). The final publication is available at http://www.springerlink.com

Published
2011
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12. Epidemiological analysis of rabies outbreaks in the Trans-Baikal Region after transboundary drift of infection

Author

Melnikova O.V. Melnikova, Shulpin M.I. Shulpin M, Stepina V.S. Stepina, Adelshin R.V. Adelshin R, Yakovchits N.V. Yakovchits, Metlin A.E. Metlin, Khankhareev S.S. Khankhareev, Kalugin D.V. Kalugin D, Shkolnikova E.N. Shkolnikova E, Chupin S.A. Chupin S, Istomina T.F. Istomina T, Ablov A.I. Ablov, Korotkova I.A. Korotkova, Bakhlina N.V. Bakhlina, Balakhonov S.V. Balakhonov S, Zarva I.D. Zarva, Andaev E.I. Andaev, Shchepin S.G. Shchepin, Zvereva O.A. Zvereva, Human Well-Being, Chita, Russia, Shoboeva R.S. Shoboeva R, Human Well-Being, Ulan-Ude, Russia, Human Well-Being, Irkutsk, Russia, Lapa S.E. Lapa S, Botvinkin A.D. Botvinkin, and Koplik М.E. Koplik М

Subjects
medicine.medical_specialty, Geography, Epidemiology, medicine, Outbreak, Rabies, medicine.disease, Virology
Published
2019
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20. Erratum. Maternal ageing impairs mitochondria! dna kinetics during early embryogenesis in mice (vol 34, pg 1313, 2019)

Author

May-Panloup, P., Brochard, Vincent, Hamel, J. F., Desquiret-Dumas, V., Chupin, S., Reynier, P., Duranthon, Véronique, Université d'Angers (UA), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Universitaire d'Angers (CHU Angers), and PRES Université Nantes Angers Le Mans (UNAM)

Subjects
early embryogenesis, [SDV]Life Sciences [q-bio], human reproduction, [INFO]Computer Science [cs], dna, mitochondrial, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019
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24. Conception et réalisation, par fabrication additive de matériaux cellulaires architecturés

Author

Heisel, C., Rochais, D., David, P., Chupin, S., Chartier, Thierry, Axe 1 : procédés céramiques (SPCTS-AXE1), Science des Procédés Céramiques et de Traitements de Surface (SPCTS), Institut des Procédés Appliqués aux Matériaux (IPAM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Ecole Nationale Supérieure de Céramique Industrielle (ENSCI)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Institut des Procédés Appliqués aux Matériaux (IPAM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Ecole Nationale Supérieure de Céramique Industrielle (ENSCI)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM), and DERORY, BEATRICE

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, ComputingMethodologies_GENERAL, [CHIM.MATE]Chemical Sciences/Material chemistry, [SPI.MAT] Engineering Sciences [physics]/Materials, ComputingMilieux_MISCELLANEOUS, [SPI.MAT]Engineering Sciences [physics]/Materials
Abstract

Prix meilleur poster; International audience

Published
2017

30. Technical decisions and design of modern compensated converting substation of oil and gas extraction complex

Author

Khokhlov, Y. I., Fyodorova, M. Y., Bakhtiev, F. F., and Chupin, S. A.

Subjects
компенсированная преобразовательная подстанция, разночастотные потребители, compensated converting substation, simulation in MATLAB, УДК 621.314.632, электроэнергетика, моделирование в MATLAB, different frequency consumers
Abstract

Приведено новое схемное решение компенсированной преобразовательной подстанции для электроснабжения буровых установок нефтегазодобывающего комплекса. В пакете MATLAB 7.5 разработана модель и осуществлено моделирование электромагнитных процессов в системе электроснабжения разночас- тотных потребителей переменного тока. Описаны основные дизайнерские решения, принятые при разработке подстанции. A new circuit decision of compensated converting substation for power supply of boring installations of an oil and gas extraction complex is shown. In the packet MATLAB 7.5 a model has been developed and simulation of electromagnetic processes in the power supply system of different frequency consumer of an alternating current has been carried out. Basic design decisions accepted while working out the substation are described. Хохлов Юрий Иванович - доктор технических наук, профессор, член-корреспондент Академии электротехнических наук РФ, декан энергетического факультета Южно- Уральского государственного университета, заведующий кафедрой «Системы электроснабжения». Специалист в области теоретических основ электротехники, силовой электроники и энергосбережения. Контактный телефон: 8-(351) 267-92-51. Khohlov Yury Ivanovich - doctor of technical sciences, professor, corresponding member of the Academy of Electrotechnical Science of the Russian Federation, Dean of the Power Engineering Faculty of South Ural State University, Head of the Power Supply Systems department, expert in the field of electrical engineering theory, power electronics and power saving systems. Contact phone: 8-(351) 267-92-51. Федорова Мария Юрьевна - старший преподаватель кафедры «Дизайн» ЮУрГУ. Специалист в области промышленного дизайна. Контактный телефон: 8-912 777 7169. Fyodorova Maria Yuryevna - senior lecturer of the Design department of South Ural State University, expert in the field of industrial design. Contact phone: 8-912 777 7169. Бахтиев Фидан Фиргатович - аспирант кафедры «Системы электроснабжения». Специалист в области силовой электроники. Контактный телефон: 8-906 865 7249. Bakhtiev Fidan Firgatovich - post-graduate student of the Power Supply Systems department of South Ural State University. The field of scientific activity is power electronics. Contact phone: 8-906 865 7249. Чупин Сергей Анатольевич - генеральный директор НТЦ «Приводная техника». Специалист в области автоматизированного электропривода. Контактный телефон: 8-912 308 0248. Chupin Sergey Anatolyevich - general director of the research and development centre «Drive technology». Specialist in the field of automated electrical drive. Contact phone: 8-912 308 0248.

Published
2009

31. A review of existing modelling methodologies for PEM fuel cell systems

Author

Astier, Stéphan, Boulon, L., Bouscayrol, Alain, Bultel, Y., Candusso, D., Caux, Stéphane, Chupin, S., Colmart, T., Coquery, G., Davat, B., De Bernardinis, A., Deseure, J., Didierjean, S., Dillet, J., Druart, F., Fadel, M., Fontes, Guillaume, François, B., Grandidier, J.-C., Harel, F., Hilairet, M., Hiraje, M., Jeme, S., Lottin, O., Madier, L., Maranzana, G., Montemaniov, S., Nguyen, Diem, Ortéga, L., Outbib, R., Pera, M.C, Raël, S., Rétière, Nicolas, Riu, Delphine, Sailler, Sébastien, Talj, R., Zhou, T., Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI ), Institut de Chimie du CNRS (INC)-Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génie Electrique de Grenoble (G2ELab), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.NRJ]Engineering Sciences [physics]/Electric power, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

32. A review on existing modelling methodologies for PEM Fuel Cell Systems

Author

François, B., Grandidier, J.C., Harel, F., Hilairet, M., Hinaje, M., Jemei, S., Lottin, O., Madier, L., Maranzana, G., Martemaniov, S., Nguyen, D., Ortega, R., Outbib, R., Pera, M.C., Raël, S., Retière, N., Riu, D., Sailler, S., Talj, R., Zhou, T., Hissel, D., Turpin, Christophe, Astier, Stéphan, Boulon, L., Bouscayrol, A., Bultel, Y., Candusso, D., Caux, Stéphane, Chupin, S., Colinart, T., Coquery, G., Davat, B., Bernardinis, A., Deseure, J., Didierjean, S., Dillet, J., Druart, F., Fadel, Maurice, Fontès, Guillaume, Pistre, Karine, Architecture et réactivité de l'ARN (ARN), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut Pprime (PPRIME), Université de Poitiers-ENSMA-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Technologies Nouvelles (INRETS/LTN), Institut National de Recherche sur les Transports et leur Sécurité (INRETS), Laboratoire de génie électrique de Paris (LGEP), Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Supérieure d'Electricité - SUPELEC (FRANCE)-Centre National de la Recherche Scientifique (CNRS), Groupe de Recherche en Electrotechnique et Electronique de Nancy (GREEN), Université de Lorraine (UL), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Mécanique et Physique des Matériaux (LMPM), Laboratoire d'études thermiques (LET), Université de Poitiers-Ecole Nationale Supérieure de Mécanique et d'Aérotechnique [Poitiers] (ISAE-ENSMA)-Centre National de la Recherche Scientifique (CNRS), Chimie Nucléaire Analytique et Bio-environnementale (CNAB), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de recherche en Electronique, Electrotechnique et Systèmes (L2ES), Institut National de Recherche sur les Transports et leur Sécurité (INRETS)-Université de Technologie de Belfort-Montbeliard (UTBM)-Université de Franche-Comté (UFC), Laboratoire d'électrotechnique de Grenoble (LEG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS), Sciences pour l'environnement (SPE), Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), Groupe ENergie Electrique et SYStémique (LAPLACE-GENESYS), LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Laboratoire d’Électrotechnique et d’Électronique de Puissance - ULR 2697 (L2EP), Centrale Lille-Université de Lille-Arts et Métiers Sciences et Technologies, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Electrochimie Interfaciale et Procédés (EIP), Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI ), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Rennes (ENS Rennes)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay)-Université Gustave Eiffel-CY Cergy Paris Université (CY), COmmande et DIAgnostic des Systèmes Electriques (LAPLACE-CODIASE), Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université de Brest (UBO), and Convertisseurs Statiques (LAPLACE-CS)

Subjects
[SPI.OTHER]Engineering Sciences [physics]/Other, [SPI.OTHER] Engineering Sciences [physics]/Other
Abstract

International audience; This paper is a common review research paper written in the framework of the "Energy program" of the French National Centre for Scientific Research (CNRS). In this program, funding has been provided to a project aiming to federate all the French competencies around fuel cell systems. Thus, as a first achievement, a review on existing modelling methodologies for PEM fuel cell systems is here proposed.

Published
2008

33. A multi-instrumented polymer exchange membrane fuel cell. Observation of the in-plane non homogeneities

Author

Maranzana, G., Olivier Lottin, Colinart, T., Chupin, S., Didierjean, S., Chauvin, Noluenn, Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), Université de Bretagne Sud (UBS)-Institut Brestois du Numérique et des Mathématiques (IBNM), and Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008

34. Etude bidimensionnelle d'une PEMFC- Analyses théorique et expérimentale, société Francaise de Chimie

Author

Colinart, T., Chupin, S., Olivier Lottin, Maranzana, G., Didierjean, S., Chauvin, Noluenn, Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), Université de Bretagne Sud (UBS)-Institut Brestois du Numérique et des Mathématiques (IBNM), and Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Published
2008

44. A review of existing modelling methodologies for PEM fuel cell systems

Author

Hissel Daniel, Christophe Turpin, Stéphan Astier, Boulon, L., Alain Bouscayrol, Bultel, Y., Candusso, D., Stéphane Caux, Chupin, S., Colmart, T., Coquery, G., Davat, B., Bernardinis, A., Deseure, J., Didierjean, S., Dillet, J., Druart, F., Maurice FADEL, Guillaume Fontes, François, B., C Grandidier, J., Harel, F., Hilairet, M., Melika Hinaje, Samir Jemei, Lottin, O., Madier, L., Martemaniov, S., Maranzana, G., Diem Nguyen, Ortéga, L., Outbib, R., Pera, M. C., Raël, S., Nicolas Rétière, Delphine Riu, Sébastien Sailler, Talj, R., and Zhou, T.

45. A review of existing modelling methodologies for PEM fuel cell systems

Author

Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI) ; CNRS - Université de Savoie - Université Joseph Fourier - Grenoble I - Institut National Polytechnique de Grenoble (INPG), Laboratoire de Génie Electrique de Grenoble (G2ELab) ; CNRS - Université Joseph Fourier - Grenoble I - Institut Polytechnique de Grenoble - Grenoble Institute of Technology, Astier, S., Boulon, L., Bouscayrol, A., Bultel, Y., Candusso, D., Caux, S., Chupin, S., Colmart, T., Coquery, G., Davat, B., De Bernardinis, A., Deseure, J., Didierjean, S., Dillet, J., Druart, F., Fadel, M., Fontes, G., François, B., Grandidier, J.C., Harel, F., Hilairet, M., Hiraje, M., Jeme, S., Lottin, O., Madier, L., Maranzana, G., Montemaniov, S., Nguyen, Diem, Ortéga, L., Outbib, R., Pera, M.C, Raël, S., Rétière, Nicolas, Riu, Delphine, Sailler, Sébastien, Talj, R., Zhou, T., Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI) ; CNRS - Université de Savoie - Université Joseph Fourier - Grenoble I - Institut National Polytechnique de Grenoble (INPG), Laboratoire de Génie Electrique de Grenoble (G2ELab) ; CNRS - Université Joseph Fourier - Grenoble I - Institut Polytechnique de Grenoble - Grenoble Institute of Technology, Astier, S., Boulon, L., Bouscayrol, A., Bultel, Y., Candusso, D., Caux, S., Chupin, S., Colmart, T., Coquery, G., Davat, B., De Bernardinis, A., Deseure, J., Didierjean, S., Dillet, J., Druart, F., Fadel, M., Fontes, G., François, B., Grandidier, J.C., Harel, F., Hilairet, M., Hiraje, M., Jeme, S., Lottin, O., Madier, L., Maranzana, G., Montemaniov, S., Nguyen, Diem, Ortéga, L., Outbib, R., Pera, M.C, Raël, S., Rétière, Nicolas, Riu, Delphine, Sailler, Sébastien, Talj, R., and Zhou, T.

Abstract

International audience

46. Metabolomic Sexual Dimorphism of the Mouse Brain is Predominantly Abolished by Gonadectomy with a Higher Impact on Females.

Author

Chabrun F, Dieu X, May-Panloup P, Chupin S, Bourreau J, Henrion D, Letournel F, Procaccio V, Bonneau D, Lenaers G, Mirebeau-Prunier D, Chao de la Barca JM, and Reynier P

Subjects
Animals, Brain, Castration, Female, Male, Metabolome, Mice, Metabolomics, Sex Characteristics
Abstract

The importance of sexual dimorphism of the mouse brain metabolome was recently highlighted, in addition to a high regional specificity found between the frontal cortex, the cerebellum, and the brain stem. To address the origin of this dimorphism, we performed gonadectomy on both sexes, followed by a metabolomic study targeting 188 metabolites in the three brain regions. While sham controls, which underwent the same surgical procedure without gonadectomy, reproduced the regional sexual dimorphism of the metabolome previously identified, no sex difference was identifiable after gonadectomy, through both univariate and multivariate analyses. These experiments also made it possible to identify which sex was responsible for the dimorphism for 35 metabolites. The female sex contributed to the difference for more than 80% of them. Our results show that gonads are the main contributors to the brain sexual dimorphism previously observed, especially in females.

Published
2021
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47. Dominant ACO2 mutations are a frequent cause of isolated optic atrophy.

Author

Charif M, Gueguen N, Ferré M, Elkarhat Z, Khiati S, LeMao M, Chevrollier A, Desquiret-Dumas V, Goudenège D, Bris C, Kane S, Alban J, Chupin S, Wetterwald C, Caporali L, Tagliavini F, LaMorgia C, Carbonelli M, Jurkute N, Barakat A, Gohier P, Verny C, Barth M, Procaccio V, Bonneau D, Zanlonghi X, Meunier I, Weisschuh N, Schimpf-Linzenbold S, Tonagel F, Kellner U, Yu-Wai-Man P, Carelli V, Wissinger B, Amati-Bonneau P, Reynier P, and Lenaers G

Abstract

Biallelic mutations in ACO2 , encoding the mitochondrial aconitase 2, have been identified in individuals with neurodegenerative syndromes, including infantile cerebellar retinal degeneration and recessive optic neuropathies (locus OPA9). By screening European cohorts of individuals with genetically unsolved inherited optic neuropathies, we identified 61 cases harbouring variants in ACO2 , among whom 50 carried dominant mutations, emphasizing for the first time the important contribution of ACO2 monoallelic pathogenic variants to dominant optic atrophy. Analysis of the ophthalmological and clinical data revealed that recessive cases are affected more severely than dominant cases, while not significantly earlier. In addition, 27% of the recessive cases and 11% of the dominant cases manifested with extraocular features in addition to optic atrophy. In silico analyses of ACO2 variants predicted their deleterious impacts on ACO2 biophysical properties. Skin derived fibroblasts from patients harbouring dominant and recessive ACO2 mutations revealed a reduction of ACO2 abundance and enzymatic activity, and the impairment of the mitochondrial respiration using citrate and pyruvate as substrates, while the addition of other Krebs cycle intermediates restored a normal respiration, suggesting a possible short-cut adaptation of the tricarboxylic citric acid cycle. Analysis of the mitochondrial genome abundance disclosed a significant reduction of the mitochondrial DNA amount in all ACO2 fibroblasts. Overall, our data position ACO2 as the third most frequently mutated gene in autosomal inherited optic neuropathies, after OPA1 and WFS1 , and emphasize the crucial involvement of the first steps of the Krebs cycle in the maintenance and survival of retinal ganglion cells., (© The Author(s) (2021). Published by Oxford University Press on behalf of the Guarantors of Brain.)

Published
2021
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48. Metabolomic signature of the seminal plasma in men with severe oligoasthenospermia.

Author

Boguenet M, Bocca C, Bouet PE, Serri O, Chupin S, Tessier L, Blanchet O, El Hachem H, Chao de la Barca JM, Reynier P, and May-Panloup P

Subjects
Adult, Amines analysis, Amino Acids analysis, Carnitine analogs & derivatives, Carnitine analysis, Fatty Acids, Unsaturated analysis, Humans, Male, Metabolomics methods, Phosphatidylcholines analysis, Prospective Studies, Semen cytology, Sphingomyelins analysis, Metabolome physiology, Oligospermia metabolism, Semen Analysis, Sperm Motility physiology, Spermatozoa pathology
Abstract

Background: Male factor is incriminated in approximately 50% of cases of infertility. The metabolomic approach has recently been used in the assessment of sperm quality and male fertility., Materials and Methods: We analyzed the metabolomic signatures of the seminal plasma in 20 men with severe oligoasthenospermia (prewash total motile sperm count < 5.10 6 ) (SOA) and compared it to 20 men with normal semen parameters, with a standardized approach of targeted and quantitative metabolomics using high-performance liquid chromatography, coupled with tandem mass spectrometry, and the Biocrates Absolute IDQ p180 kit., Results: Among the 188 metabolites analyzed, 110 were accurately measured in the seminal plasma. A robust model discriminating the two populations (Q 2(cum) = 55.2%) was obtained by OPLS-DA (orthogonal partial least-squares discriminant analysis), based on the drop in concentrations of 37 metabolites with a VIP (variable important for projection) greater than 1. Overall, in men with SOA, there was a significant decrease in: 17 phosphatidylcholines and four sphingomyelins; acylcarnitines, with free L-carnitine being the most discriminating metabolite; polyunsaturated fatty acids; six amino acids (glutamate, aspartate, methionine, tryptophan, proline, and alanine); and four biogenic amines (spermine, spermidine, serotonin, and alpha-aminoadipate)., Discussion: Our signature includes several metabolic changes with different impacts on the sperm quality: a change in phospholipid composition and the saturation of their fatty acids that is potentially linked to the deterioration of sperm membranes; a carnitine deficiency that can negatively impact the energy production via fatty acid oxidation and oxidative phosphorylation; and a decreased level of amino acids and biogenic amines that can lead to dysregulated metabolic and signaling pathways., Conclusion: We provide a global overview of the metabolic defects contributing to the structural and functional alteration of spermatozoa in severe oligoasthenospermia. These findings offer new insights into the pathophysiology of male factor infertility that could help to develop future specific treatments., (© 2020 American Society of Andrology and European Academy of Andrology.)

Published
2020
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49. Metabolomics hallmarks OPA1 variants correlating with their in vitro phenotype and predicting clinical severity.

Author

Chao de la Barca JM, Fogazza M, Rugolo M, Chupin S, Del Dotto V, Ghelli AM, Carelli V, Simard G, Procaccio V, Bonneau D, Lenaers G, Reynier P, and Zanna C

Subjects
Alleles, Animals, Fibroblasts metabolism, Humans, Mice, Mutation, Missense genetics, Phenotype, Proteostasis genetics, Endoplasmic Reticulum Stress genetics, GTP Phosphohydrolases genetics, Metabolomics
Abstract

Interpretation of variants of uncertain significance is an actual major challenge. We addressed this question on a set of OPA1 missense variants responsible for variable severity of neurological impairments. We used targeted metabolomics to explore the different signatures of OPA1 variants expressed in Opa1 deleted mouse embryonic fibroblasts (Opa1-/- MEFs), grown under selective conditions. Multivariate analyses of data discriminated Opa1+/+ from Opa1-/- MEFs metabolic signatures and classified OPA1 variants according to their in vitro severity. Indeed, the mild p.I382M hypomorphic variant was segregating close to the wild-type allele, while the most severe p.R445H variant was close to Opa1-/- MEFs, and the p.D603H and p.G439V alleles, responsible for isolated and syndromic presentations, respectively, were intermediary between the p.I382M and the p.R445H variants. The most discriminant metabolic features were hydroxyproline, the spermine/spermidine ratio, amino acid pool and several phospholipids, emphasizing proteostasis, endoplasmic reticulum (ER) stress and phospholipid remodeling as the main mechanisms ranking OPA1 allele impacts on metabolism. These results demonstrate the high resolving power of metabolomics in hierarchizing OPA1 missense mutations by their in vitro severity, fitting clinical expressivity. This suggests that our methodological approach can be used to discriminate the pathological significance of variants in genes responsible for other rare metabolic diseases and may be instrumental to select possible compounds eligible for supplementation treatment., (© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

Published
2020
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50. Metabolomic Profiling of Plasma and Erythrocytes in Sickle Mice Points to Altered Nociceptive Pathways.

Author

Dembélé KC, Mintz T, Veyrat-Durebex C, Chabrun F, Chupin S, Tessier L, Simard G, Henrion D, Mirebeau-Prunier D, Chao de la Barca JM, Tharaux PL, and Reynier P

Subjects
Animals, Discriminant Analysis, Hemoglobin, Sickle, Heterozygote, Least-Squares Analysis, Mice, Principal Component Analysis, Anemia, Sickle Cell blood, Anemia, Sickle Cell metabolism, Erythrocytes metabolism, Metabolomics, Nociception
Abstract

Few data-driven metabolomic approaches have been reported in sickle cell disease (SCD) to date. We performed a metabo-lipidomic study on the plasma and red blood cells of a steady-state mouse model carrying the homozygous human hemoglobin SS, compared with AS and AA genotypes. Among the 188 metabolites analyzed by a targeted quantitative metabolomic approach, 153 and 129 metabolites were accurately measured in the plasma and red blood cells, respectively. Unsupervised PCAs (principal component analyses) gave good spontaneous discrimination between HbSS and controls, and supervised OPLS-DAs (orthogonal partial least squares-discriminant analyses) provided highly discriminant models. These models confirmed the well-known deregulation of nitric oxide synthesis in the HbSS genotype, involving arginine deficiency and increased levels of dimethylarginines, ornithine, and polyamines. Other discriminant metabolites were newly evidenced, such as hexoses, alpha-aminoadipate, serotonin, kynurenine, and amino acids, pointing to a glycolytic shift and to the alteration of metabolites known to be involved in nociceptive pathways. Sharp remodeling of lysophosphatidylcholines, phosphatidylcholines, and sphingomyelins was evidenced in red blood cells. Our metabolomic study provides an overview of the metabolic remodeling induced by the sickle genotype in the plasma and red blood cells, revealing a biological fingerprint of altered nitric oxide, bioenergetics and nociceptive pathways., Competing Interests: The authors declare no conflict of interest.

Published
2020
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