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The etiology of the mealybug wit of pineapple (MWP) disease is associated with a complex of ampeloviruses (Closteroviridae) named Pineapple mealybug wilt-associated virus -1 to -5 (PMWaV-1 to -5). The aims of this study were to determine the specie of PMWaVs associated with MWP symptoms in pineapples plants hybrid 'MD-2', and virus presence in asymptomatic crowns of the same cultivar. Six symptomatic plants and 25 asymptomatic crowns were collected in pineapples cv. 'MD-2' commercial fields in Ciego de Ávila, during the period 2010-2015. The crowns were used as explants to produce "in vitro" plants. Total RNAs were extracted to detect PMWaV-1, -2 and -3 by RT-PCR. Five of the six MWP symptomatic plants contained PMWaV-2 infection, four of them with mixed infection with PMWaV-1 and/or PMWaV-3. The forty percent of the 25 in vitro plants were infected, at least, by one PMWaV, 16 % of the three viral species, 8 % by two, and 16 % by only one virus. The incidence of PMWaVs was 38.5 % either for PMWaV-1 and PMWaV-3 (12/31), and 32.3 % for PMWaV-2 (10/31). These results indicate the need of the sanitary certification of pineapple propagation material. [ABSTRACT FROM AUTHOR]