4 results on '"Cindy M López"'
Search Results
2. Genomic prediction of strawberry resistance to postharvest fruit decay caused by the fungal pathogenBotrytis cinerea
- Author
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Randi A. Famula, Cindy M. López, Barbara Blanco-Ulate, Dominique D A Pincot, Stefan Petrasch, Mitchell J. Feldmann, Michael A. Hardigan, Steven J. Knapp, Glenn S. Cole, Saskia D. Mesquida-Pesci, and Jannink, J-L more...
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AcademicSubjects/SCI01140 ,disease resistance ,AcademicSubjects/SCI00010 ,whole-genome regression ,Titratable acid ,QH426-470 ,Biology ,AcademicSubjects/SCI01180 ,Shelf life ,Genome ,Fragaria ,Botrytis cinerea ,Genetic variation ,Genetics ,Molecular Biology ,Genetics (clinical) ,Mycelium ,Plant Diseases ,Investigation ,genome-wide association study ,Human Genome ,Genomics ,Heritability ,biology.organism_classification ,necrotroph ,Horticulture ,Infectious Diseases ,Fruit ,breeding ,Postharvest ,AcademicSubjects/SCI00960 ,Botrytis ,Fragaria × ananassa ,Fragaria x ananassa - Abstract
Gray mold, a disease of strawberry (Fragaria×ananassa) caused by the ubiquitous necrotrophBotrytis cinerea, renders fruit unmarketable and causes economic losses in the postharvest supply chain. To explore the feasibility of selecting for increased resistance to gray mold, we undertook genetic and genomic prediction studies in strawberry populations segregating for fruit quality and shelf life traits hypothesized to pleiotropically affect susceptibility. As predicted, resistance to gray mold was heritable but quantitative genetically complex. While every individual was susceptible, the speed of symptom progression and severity differed. Narrow-sense heritability ranged from 0.38-0.71 for lesion diameter (LD) and 0.39-0.44 for speed of emergence of external mycelium (EM). Even though significant additive genetic variation was genome wide observed for LD and EM, the phenotypic ranges were comparatively narrow and genome-wide analyses did not identify any large effect loci. Genomic selection accuracy ranged from 0.28-0.59 for LD and 0.37-0.47 for EM. Additive genetic correlations between fruit quality and gray mold resistance traits were consistent with prevailing hypotheses: LD decreased as titratable acidity increased, whereas EM increased as soluble solid whole genome content decreased and firmness increased. We concluded that phenotypic and genomic selection could be regression effective for reducing LD and increasing EM, especially in long shelf life populations, but that a significant fraction of the genetic variation for resistance to gray mold was caused by the pleiotropic effects of fruit quality traits that differ among market and shelf life classes. more...
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- 2021
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- View/download PDF
Catalog
3. Elucidating dynamics and regulation of alternative splicing in osteogenic differentiation
- Author
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Yacoubian, Henrich M, Cindy M. López, Lan Lin, Samir Adhikari, Chun Rf, Jaquesta Adams, Yibin Wang, and Yi Xing
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Cell fate commitment ,Exon ,Cellular differentiation ,RNA splicing ,Alternative splicing ,Biology ,Progenitor cell ,Gene ,Exon skipping ,Cell biology - Abstract
Nearly all human multi-exonic genes undergo alternative splicing (AS) via regulation by RNA-binding proteins (RBPs), but few studies have examined the temporal dynamics of AS and its regulation during cell differentiation in the bone niche. We sought to evaluate how AS, under the control of RBPs, affects cell fate commitment during induced osteogenic differentiation of human bone marrow-derived multipotent stem/stromal progenitor cells (MSPCs). We generated a time-course RNA sequencing (RNA-seq) dataset representative of induced MSPC differentiation to osteoblasts. Our analysis revealed widespread AS changes, coordinated with differential RBP expression, at multiple time points, including many AS changes in non-differentially expressed genes. We also developed a computational approach to profile the dynamics and regulation of AS by RBPs using time-course RNA-seq data, by combining temporal patterns of exon skipping and RBP expression with RBP binding sites in the vicinity of regulated exons. In total we identified nine RBPs as potential key splicing regulators during MSPC osteogenic differentiation. Perturbation of one candidate,KHDRBS3, inhibited osteogenesis and bone formationin vitro, validating our computational prediction of “driver” RBPs. Overall, our work highlights a high degree of complexity in the splicing regulation of MSPC osteogenic differentiation. Our computational approach may be applied to other time-course data to explore dynamic AS changes and associated regulatory mechanisms in other biological processes or disease trajectories. more...
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- 2020
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4. Multi-Dimensional Machine Learning Approaches for Fruit Shape Recognition and Phenotyping in Strawberry
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Randi A. Famula, Steven J. Knapp, Mitchell J. Feldmann, Michael A. Hardigan, Amy Tabb, Glenn S. Cole, and Cindy M. López
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business.industry ,Computer science ,Ordinal Scale ,Quantitative trait locus ,Machine learning ,computer.software_genre ,Digital image ,Variable (computer science) ,Multi dimensional ,Feature (machine learning) ,Unsupervised learning ,Artificial intelligence ,business ,computer ,Categorical variable - Abstract
BackgroundShape is a critical element of the visual appeal of strawberry fruit and determined by both genetic and non-genetic factors. Current fruit phenotyping approaches for external characteristics in strawberry rely on the human eye to make categorical assessments. However, fruit shape is multi-dimensional, continuously variable, and not adequately described by a single quantitative variable. Morphometric approaches enable the study of complex forms but are often abstract and difficult to interpret. In this study, we developed a mathematical approach for transforming fruit shape classifications from digital images onto an ordinal scale called the principal progression of k clusters (PPKC). We use these human-recognizable shape categories to select features extracted from multiple morphometric analyses that are best fit for genome-wide and forward genetic analyses.ResultsWe transformed images of strawberry fruit into human-recognizable categories using unsupervised machine learning, discovered four principal shape categories, and inferred progression using PPKC. We extracted 67 quantitative features from digital images of strawberries using a suite of morphometric analyses and multi-variate approaches. These analyses defined informative feature sets that effectively captured quantitative differences between shape classes. Classification accuracy ranged from 68.9 – 99.3% for the newly created, genetically correlated phenotypic variables describing a shape.ConclusionsOur results demonstrated that strawberry fruit shapes could be robustly quantified, accurately classified, and empirically ordered using image analyses, machine learning, and PPKC. We generated a dictionary of quantitative traits for studying and predicting shape classes and identifying genetic factors underlying phenotypic variability for fruit shape in strawberry. The methods and approaches we applied in strawberry should apply to other fruits, vegetables, and specialty crops. more...
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- 2019
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