Search

Your search keyword '"Ciprì, V."' showing total 6 results
Start Over Author "Ciprì, V."
6 results on '"Ciprì, V."'

1. Development and clinical trial of a DNA vaccine as immunotherapy during Leishmaniasis

Author

MANNA, LAURA, GRAVINO, ANGELO ELIO, Piras I.M., Ciprì V., Alberti A., Della Peruta L., Del Pizzo C. M, Gammarano N., Coradduzza E., Cacciotto C., Pittau M., Chessa B., Società Italiana Parassitologia, Manna, Laura, Piras, I. M., Ciprì, V., Alberti, A., Della Peruta, L., Del Pizzo, C. M., Gammarano, N., Coradduzza, E., Cacciotto, C., Pittau, M., Gravino, ANGELO ELIO, and Chessa, B.

Published
2012

2. Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily

Author

Reale S., Lupo T., Migliazzo A., Di Mauro C., Ciprì V., Calderone S., Vitale F., MANNA, LAURA, Reale, S., Lupo, T., Migliazzo, A., Di Mauro, C., Ciprì, V., Calderone, S., Manna, Laura, and Vitale, F.

Subjects
Dogs, Polymorphism, Genetic, Animals, Dog Diseases, Leishmania infantum, Sicily, Microsatellite Repeats
Abstract

Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantum from dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantum isolates within each zymodema. Model- and distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.

Published
2010

3. Identificazione dei ceppi di Leishmania mdiante analisi dei microsatelliti

Author

Reale S., Lupo T., Rea S., Migliazzo A., Piazza M., Ciprì V., Vitale F., MANNA, LAURA, GRAVINO, ANGELO ELIO, Società di Diagnostica di Laboratorio Veterinaria, Reale, S., Lupo, T., Manna, Laura, Gravino, ANGELO ELIO, Rea, S., Migliazzo, A., Piazza, M., Ciprì, V., and Vitale, F.

Published
2008

4. Multilocus Microsatellite Polymorphism Analysis to Characterize Leishmania infantumStrains Isolated in Sicily

Author

Reale, S., Lupo, T., Migliazzo, A., Di Mauro, C., Ciprì, V., Calderone, S., Manna, L., and Vitale, F.

Abstract

Different approaches are being developed to improve the differentiation of Leishmaniagenus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantumisolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantumfrom dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantumisolates within each zymodema. Model- and distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.

Published
2010
Full Text
View/download PDF

5. Development and clinical trial of a novel DNA vaccine as immunotherapy during canine Leishmaniasis

Author

Laura Manna, Italia Della Peruta, Angelo Elio Gravino, Elisabetta Coradduzza, Nicoletta Gammarano, Carla Cacciotto, Bernardo Chessa, Alberto Alberti, Marco Pittau, Carlo Maria Del Pizzo, Ilaria Michela Piras, Valentina Ciprì, Manna L., Piras I.M., Cipri V., Alberti A., Peruta I.D., Del Pizzo C.M., Gammarano N., Coradduzza E., Cacciotto C., Pittau M., Gravino A.E., Chessa B., Manna, Laura, P. i. r. a. s., . I. M, Ciprì, V, Alberti, A, Della Peruta, I, Del P. i. z. z. o., . C. M, Gammarano, N, Coradduzza, E, Cacciotto, C, Pittau, M, Gravino, ANGELO ELIO, and Chessa, B.

Subjects
DNA vaccine, medicine.medical_treatment, Immunology, DNA vaccination, Virology, Drug Discovery, Canine leishmaniasis, medicine, Dog, Immunology and Allergy, Leishmania infantum, Cytokine, biology, business.industry, Immunotherapy, medicine.disease, biology.organism_classification, Leishmania, Clinical trial, Real-time polymerase chain reaction, Visceral leishmaniasis, business, VET/05 Malattie infettive degli animali domestici, Real-time PCR
Abstract

Background: Visceral Leishmaniasis (VL) is a zoonotic disease caused by Leishmaniainfantum in the Mediterranean area. Dogs are the main reservoir of Leishmaniainf antum parasites. Disease management represents a serious problem, since anti-Leishmania drugs have limited efficacy in both symptomatic and asymptomatic dogs, which are infective to phlebotomine vectors. In many tropical and sub-tropical countries the development of a safe and easily-available vaccine has high priority. DNA vaccines represent one of the most recent innovations in the field of immunization. Findings: This study aimed to evaluate the effect of a DNA vaccine based on two Leishmania antigens (Cpb1, PO) in leishmaniotic dogs. Twelve leishmaniotic dogs from a Leishmaniasis-endemic area (Naples, Italy) received three consecutive injections of DNA vaccine at 15-days intervals. Another group of five leishmaniotic dogs received the same amount of pVAX-1 without the coding sequences of Leishmania antigens. Leishmania DNA load, INFγ, IL-4 mRNA expression levels and clinical parameters were tested before and after the therapy, every 3 months for a period of 12 months. Analysis of the data in the vaccinated dogs showed: i) a decrease Leishmania DNA load in lymph node samples, ii) an increase of INFγ and IL-4 mRNA expression levels in PBMC samples. All vaccinated dogs also showed an improvement in the clinical symptoms. Conclusion: Our results show that the vaccine developed in this study may represent a useful tool in the treatment of leishmaniotic dogs. However, since the duration of positive effects is limited in time, further trials are needed to evaluate the effectiveness of immunotherapy alone or in association with conventional therapy.

Published
2012

6. Residues of 165 pesticides in citrus fruits using LC-MS/MS: a study of the pesticides distribution from the peel to the pulp.

Author

Calvaruso E, Cammilleri G, Pulvirenti A, Lo Dico GM, Lo Cascio G, Giaccone V, Vitale Badaco V, Ciprì V, Alessandra MM, Vella A, Macaluso A, Di Bella C, and Ferrantelli V

Subjects
Chromatography, Liquid methods, Fungicides, Industrial analysis, Sicily, Tandem Mass Spectrometry methods, Citrus chemistry, Fruit chemistry, Pesticide Residues analysis
Abstract

A sensitive LC-ESI-MS/MS method was developed for the determination of 165 pesticides in 50 citrus fruit samples collected in Sicily. Moreover, an evaluation of pesticides levels in the citrus layers (peel, albedo, and pulp) was carried out. The method presented acceptable trueness, precision, and linearity with LOQ of 5 μg/kg. The results obtained showed a high frequency of fungicides class pesticides in all the citrus samples examined (>95%) with the highest concentrations in the peel (4468 µg/Kg). A significant difference of concentrations was found between the layers of the citrus fruits analysed (p < 0.05). In particular, the peel and albedo present higher pesticides significantly higher than the pulp. Our findings confirming the widespread use of these substances in citrus cultivation and suggesting the importance of pesticides analysis in all the citrus fruit layers separately, considering the different interactions between the physicochemical characteristics of the matrices and the pesticides.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results