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Your search keyword '"Ciutti, Arianna"' showing total 22 results
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22 results on '"Ciutti, Arianna"'

2. NMR structure of alpha-bungarotoxin free and bound to a mimotope of the nicotinic acetylcholine receptor

Author

Scarcelli, Maria, Spiga, Ottavia, Ciutti, Arianna, Bernini, Andrea, Bracci, Luisa, Lelli, Barbara, Lozzi, Luisa, Calamandrei, Duccio, Di Maro, Daniela, Klein, Samuel, and Niccolai, Neri

Subjects
Biochemistry -- Research, Toxins -- Physiological aspects, Acetylcholine -- Physiological aspects, Nicotinic receptors -- Physiological aspects, Peptides -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the synthetic peptides mimicking the snake neurotoxin binding site of the nicotinic receptors. The use of the combinatorial library approach in producing these peptides is described.

Published
2002

14. NMR studies of BPTI aggregation by using paramagnetic relaxation reagents

Author

Bernini, Andrea, primary, Spiga, Ottavia, additional, Ciutti, Arianna, additional, Venditti, Vincenzo, additional, Prischi, Filippo, additional, Governatori, Mariangela, additional, Bracci, Luisa, additional, Lelli, Barbara, additional, Pileri, Silvia, additional, Botta, Mauro, additional, Barge, Alessandro, additional, Laschi, Franco, additional, and Niccolai, Neri, additional

Published
2006
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15. Structurally Driven Selection of Human Hepatitis C Virus Mimotopes

Author

Spiga, Ottavia, primary, Padula, Maria G, additional, Scarselli, Maria, additional, Ciutti, Arianna, additional, Bernini, Andrea, additional, Venditti, Vincenzo, additional, Prischi, Filippo, additional, Falciani, Chiara, additional, Lozzi, Luisa, additional, Bracci, Luisa, additional, Valensin, Piero E, additional, Caudai, Cinzia, additional, and Niccolai, Neri, additional

Published
2005
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20. Structurally Driven Selection of Human Hepatitis C Virus Mimotopes

Author

Spiga, Ottavia, Padula, Maria G, Scarselli, Maria, Ciutti, Arianna, Bernini, Andrea, Venditti, Vincenzo, Prischi, Filippo, Falciani, Chiara, Lozzi, Luisa, Bracci, Luisa, Valensin, Piero E, Caudai, Cinzia, and Niccolai, Neri

Abstract

A structural genomics approach is proposed for the development of new diagnostic kits. It combines molecular modelling, peptide synthesis and immunological tests. The preliminary step is the development of a reliable three-dimensional structure of an immunodominant protein of the target pathogenic organism using the various bioinformatic strategies that are now available to structural biologists. Once the protein structure is obtained, the most surface-exposed fragments with minimal sequence variability among the different strains reported in the genomic data bank are reproduced synthetically as linear peptides. These peptides are then tested for immunoreactivity with the plasma of infected patients to determine whether the synthetic molecules have antigenic activity and can therefore be used to detect infecting agents. This structurally driven selection of mimotopes was successfully performed for the human hepatitis C virus, as five peptides that specifically interact with the plasma of HCV-infected patients were identified solely on the basis of the three-dimensional structure predicted for the E2 homodimer of the 1a viral subtype. A similar approach could easily be extended to a large variety of immunogenic proteins from other pathogenic organisms.

Published
2006
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22. Structurally driven selection of human hepatitis C virus mimotopes

Author

Spiga, Ottavia, Padula, Maria G., Scarselli, Maria, Ciutti, Arianna, Bernini, Andrea, Venditti, Vincenzo, Prischi, Filippo, Falciani, Chiara, LUISA LOZZI, Bracci, Luisa, Valensin, Piero E., Caudai, Cinzia, and Niccolai, Neri

Subjects
Models, Molecular, Pharmacology, Genes, Viral, Immune Sera, Molecular Sequence Data, Enzyme-Linked Immunosorbent Assay, Hepacivirus, Hepatitis C, Viral Proteins, Infectious Diseases, Species Specificity, Viral Envelope Proteins, Humans, Pharmacology (medical), Selection, Genetic, Peptides, Sequence Alignment
Abstract

A structural genomics approach is proposed for the development of new diagnostic kits. It combines molecular modelling, peptide synthesis and immunological tests. The preliminary step is the development of a reliable three-dimensional structure of an immunodominant protein of the target pathogenic organism using the various bioinformatic strategies that are now available to structural biologists. Once the protein structure is obtained, the most surface-exposed fragments with minimal sequence variability among the different strains reported in the genomic data bank are reproduced synthetically as linear peptides. These peptides are then tested for immunoreactivity with the plasma of infected patients to determine whether the synthetic molecules have antigenic activity and can therefore be used to detect infecting agents. This structurally driven selection of mimotopes was successfully performed for the human hepatitis C virus, as five peptides that specifically interact with the plasma of HCV-infected patients were identified solely on the basis of the three-dimensional structure predicted for the E2 homodimer of the 1a viral subtype. A similar approach could easily be extended to a large variety of immunogenic proteins from other pathogenic organisms.

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