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18 results on '"Clément, Franck"'

5. Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes.

Author

Lefrançois, Pauline, Girard‐Sahun, Fanny, Badets, Vasilica, Clément, Franck, and Arbault, Stéphane

Subjects
MICROELECTRODES, REACTIVE oxygen species, SUPEROXIDES, ANIONS, ULTRAMICROELECTRODES, OXYGEN reduction
Abstract

The reactivity of platinized ultramicroelectrodes (Pt‐black UMEs) towards superoxide anion O2.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O2, was studied. Voltammetric studies in PBS demonstrate that Pt‐black UMEs provide: i) a well‐resolved reversible redox signature for O2.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt‐black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt‐black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico‐chemical investigations. [ABSTRACT FROM AUTHOR]

Published
2021
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7. Correlations between gaseous and liquid phase chemistries induced by cold atmospheric plasmas in a physiological buffer

Author

Girard, Fanny, primary, Peret, Mathieu, additional, Dumont, Natacha, additional, Badets, Vasilica, additional, Blanc, Sylvie, additional, Gazeli, Kristaq, additional, Noël, Cédric, additional, Belmonte, Thierry, additional, Marlin, Laurent, additional, Cambus, Jean-Pierre, additional, Simon, Guillaume, additional, Sojic, Neso, additional, Held, Bernard, additional, Arbault, Stéphane, additional, and Clément, Franck, additional

Published
2018
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10. Cold Atmospheric Plasma Induces a Predominantly Necrotic Cell Death via the Microenvironment

Author

Virard, François, Cousty, Sarah, Cambus, Jean-Pierre, Valentin, Alexis, Kémoun, Philippe, Clément, Franck, UFR d’Odontologie, Université de Lyon, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Centre d'investigation clinique de Toulouse (CIC 1436), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de Rangueil, CHU Toulouse [Toulouse], Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Pôle Santé publique et médecine publique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3)

Subjects
Fibroblasts / drug effects, Plasma Gases, [SPI.PLASMA]Engineering Sciences [physics]/Plasmas, lcsh:R, Fibroblasts / cytology, Reactive Oxygen Species / metabolism, lcsh:Medicine, Apoptosis, [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, Fibroblasts, Apoptosis / drug effects, Necrosis, Atmospheric Pressure, Necrosis / chemically induced, Humans, lcsh:Q, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, Cells Cultured, Reactive Oxygen Species, lcsh:Science, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology, Research Article, Plasma Gases / pharmacology
Abstract

International audience; Introduction: Cold plasma is a partially ionized gas generated by an electric field at atmospheric pressure that was initially used in medicine for decontamination and sterilization of inert surfaces. There is currently growing interest in using cold plasma for more direct medical applications, mainly due to the possibility of tuning it to obtain selective biological effects in absence of toxicity for surrounding normal tissues. While the therapeutic potential of cold plasma in chronic wound, blood coagulation, and cancer treatment is beginning to be documented, information on plasma/cell interaction is so far limited and controversial. Methods and Results: Using normal primary human fibroblast cultures isolated from oral tissue, we sought to decipher the effects on cell behavior of a proprietary cold plasma device generating guided ionization waves carried by helium. In this model, cold plasma treatment induces a predominantly necrotic cell death. Interestingly, death is not triggered by a direct interaction of the cold plasma with cells, but rather via a transient modification in the microenvironment. We show that modification of the microenvironment redox status suppresses treatment toxicity and protects cells from death. Moreover, necrosis is not accidental and seems to be an active response to an environmental cue, as its execution can be inhibited to rescue cells. Conclusion: These observations will need to be taken into account when studying in vitro plasma/cell interaction and may have implications for the design and future evaluation of the efficacy and safety of this new treatment strategy. © 2015 Virard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Published
2015
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11. Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines

Author

Dezest, Marlène, primary, Chavatte, Laurent, additional, Bourdens, Marion, additional, Quinton, Damien, additional, Camus, Mylène, additional, Garrigues, Luc, additional, Descargues, Pascal, additional, Arbault, Stéphane, additional, Burlet-Schiltz, Odile, additional, Casteilla, Louis, additional, Clément, Franck, additional, Planat, Valérie, additional, and Bulteau, Anne-Laure, additional

Published
2017
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12. How Do Gas Hydrates Spread on a Substrate?

Author

Martínez de Baños, María Lourdes, primary, Hobeika, Nelly, additional, Bouriat, Patrick, additional, Broseta, Daniel, additional, Enciso, Eduardo, additional, Clément, Franck, additional, and Brown, Ross, additional

Published
2016
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15. Plasma Process. Polym. 10∕2013

Author

Duday, David, primary, Clément, Franck, additional, Lecoq, Elodie, additional, Penny, Christian, additional, Audinot, Jean-Nicolas, additional, Belmonte, Thierry, additional, Kutasi, Kinga, additional, Cauchie, Henry-Michel, additional, and Choquet, Patrick, additional

Published
2013
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17. Atmospheric Pressure Dielectric Barrier Discharges Under Unipolar and Bipolar HV Excitation in View of Chemical Reactivity in Afterglow Conditions.

Author

Panousis, Emanuel, Merbahi, Nofel, Clément, Franck, Ricard, Andre, Yousfi, Mohammed, Papageorghiou, Leberis, Loiseau, Jean-Francis, Eichwald, Olivier, Held, Bernard, and Spyrou, Nicolas

Subjects
ATMOSPHERIC pressure, DIELECTRICS, COLLISIONAL excitation, CHEMICAL reactions, AFTERGLOW (Physics), NITROGEN
Abstract

This paper deals with atmospheric pressure DBDs driven under unipolar and bipolar pulsed excitation, in a con- text of chemical reactivity in flowing afterglow conditions. The resulting discharges in air and nitrogen are examined via electrical and optical diagnostics. Then, their yield in the production of active species (ozone for the air afterglow-atomic Nitrogen and N2 (A) metastables for the N2 afterglow) is compared. An effort is made to illustrate the key discharge parameters that are likely to influence the concentrations of neutral active species in afterglow conditions. [ABSTRACT FROM AUTHOR]

Published
2009
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18. Experimental investigation of an atmospheric pressure DBD under bi-polar pulsed high voltage excitation

Author

Panousis, E., Clement, Franck, Lecoq, E., Loiseau, J.-F., Held, B., Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Clément, Franck

Subjects
[SPI.PLASMA]Engineering Sciences [physics]/Plasmas, [SPI.PLASMA] Engineering Sciences [physics]/Plasmas
Abstract

International audience; This work presents a primary experimental study of a custom-made coaxial DBD reactor driven by a bipolar pulsed High Voltage supply. The reactor is fed by nitrogen or air at atmospheric pressure conditions and uses a dielectric coating on the HV electrode. The High Voltage generator delivers a "pulse-like" bipolar signal in the 10-50 kHz frequency range. This set-up has been conceived for surface treatment applications in afterglow conditions. Electrical and optical diagnostics are employed for the investigation of the nitrogen and air DBD while varying the frequency of the HV bipolar signal and the maximum applied voltage. Current and voltage oscillogrammes are used in order to derive the mean plasma power injected in the gap. Photomultiplier scans reveal the discharge's filamentary character whereas Optical Emission Spectroscopy (OES) shows the excited species present in such conditions. Comparisons between electrical and OES results show a correlation between the mean plasma power and the production of active species.

Published
2008

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