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126 results on '"Classen B"'

2. Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Author

Pfeifer, L, Shafee, T, Johnson, KL, Bacic, A, Classen, B, Pfeifer, L, Shafee, T, Johnson, KL, Bacic, A, and Classen, B

Abstract

Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat. This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment. Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest. AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies. Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy. Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs. The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues. Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids. These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties. The role of this polyanionic surface is discussed with regard to adaption to the marine environment.

Published
2020

3. Immunomodulatory effects of arabinogalactan-proteins from Baptisia and Echinacea

Author

Classen, B., Thude, S., Blaschek, W., Wack, M., and Bodinet, C.

Subjects
Mimosaceae -- Health aspects -- Research, Legumes -- Health aspects -- Research, Immunological adjuvants -- Research, Medicinal plants -- Research -- Health aspects, Immunopharmacology -- Research -- Health aspects, Beans -- Health aspects -- Research, Echinacea -- Health aspects -- Research, Biological sciences, Health, Science and technology
Abstract

Abstract The influences of different arabinogalactan-proteins (AGPs) on proliferation and IgM-production of mouse lymphocytes as well as nitrite- and IL6-production of mouse macrophages were investigated in vitro. AGPs have been [...]

Published
2006

6. Vasoconstrictor stimulus determines the functional contribution of myoendothelial feedback to mesenteric arterial tone

Author

Wei, R., Lunn, S. E., Tam, R., Gust, S. L., Classen, B., Kerr, P. M., and Plane, F.

Subjects
Feedback, Physiological, Male, Myocytes, Smooth Muscle, Cardiovascular, Intermediate-Conductance Calcium-Activated Potassium Channels, Membrane Potentials, Mesenteric Arteries, Rats, Rats, Sprague-Dawley, Norepinephrine, Vasoconstriction, 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid, Animals, Vasoconstrictor Agents, Endothelium, Vascular, Cells, Cultured
Abstract

KEY POINTS: In isolated resistance arteries, endothelial modulation of vasoconstrictor responses to α(1)‐adrenoceptor agonists occurs via a process termed myoendothelial feedback: localized inositol trisphosphate (InsP(3))­dependent Ca(2+) transients activate intermediate conductance Ca(2+)­activated K(+) (IK(Ca)) channels, hyperpolarizing the endothelial membrane potential to limit further reductions in vessel diameter. We demonstrate that IK(Ca) channel‐mediated myoendothelial feedback limits responses of isolated mesenteric arteries to noradrenaline and nerve stimulation, but not to the thromboxane A(2) mimetic U46619 or to increases in intravascular pressure. In contrast, in the intact mesenteric bed, although responses to exogenous noradrenaline were limited by IK(Ca) channel‐mediated myoendothelial feedback, release of NO and activation of endothelial small conductance Ca(2+)­activated K(+) (SK(Ca)) channels in response to increases in shear stress appeared to be the primary mediators of endothelial modulation of vasoconstriction. We propose that (1) the functional contribution of myoendothelial feedback to arterial tone is determined by the nature of the vasoconstrictor stimulus, and (2) although IK(Ca) channel‐mediated myoendothelial feedback may contribute to local control of arterial diameter, in the intact vascular bed, increases in shear stress may be the major stimulus for engagement of the endothelium during vasoconstriction. ABSTRACT: Constriction of isolated resistance arteries in response to α(1)‐adrenoceptor agonists is limited by reciprocal engagement of inhibitory endothelial mechanisms via myoendothelial feedback. In the current model of feedback, agonist stimulation of smooth muscle cells results in localized InsP(3)­dependent Ca(2+) transients that activate endothelial IK(Ca) channels. The subsequent hyperpolarization of the endothelial membrane potential then feeds back to the smooth muscle to limit further reductions in vessel diameter. We hypothesized that the functional contribution of InsP(3)–IK(Ca) channel‐mediated myoendothelial feedback to limiting arterial diameter may be influenced by the nature of the vasoconstrictor stimulus. To test this hypothesis, we investigated the functional role of myoendothelial feedback in modulating responses of rat mesenteric resistance arteries to the adrenoceptor agonist noradrenaline, the thromboxane A(2) mimetic U46619, increases in intravascular pressure and stimulation of perivascular sympathetic nerves. In isolated arteries, responses to noradrenaline and stimulation of sympathetic nerves, but not to U46619 and increases in intravascular pressure, were modulated by IK(Ca) channel‐dependent myoendothelial feedback. In the intact mesenteric bed perfused under conditions of constant flow, responses to exogenous noradrenaline were modulated by myoendothelial feedback, but shear stress‐induced release of NO and activation of endothelial SK(Ca) channels appeared to be the primary mediators of endothelial modulation of vasoconstriction to agonists and nerve stimulation. Thus, we propose that myoendothelial feedback may contribute to local control of diameter within arterial segments, but at the level of the intact vascular bed, increases in shear stress may be the major stimulus for engagement of the endothelium during vasoconstriction.

Published
2018

10. Patientendatenmanagementsysteme (PDMS) - Werkzeuge zum Qualitätsmanagement in der Intensivmedizin?

Author

Classen B and Friesdorf W

Subjects
Pediatrics, medicine.medical_specialty, Quality management, business.industry, Public health, Medical record, General Medicine, Patient data, Critical Care and Intensive Care Medicine, medicine.disease, Anesthesiology and Pain Medicine, Intensive care, Management system, Emergency Medicine, Information system, Medicine, Medical emergency, business
Published
1997
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12. Peptide NMHRYPNQ of the Cellular Prion Protein (PrPC) Inhibits Aggregation and Is a Potential Key for Understanding Prion-Prion Interactions

Author

Rehders, Dirk, Classen, B., Redecke, L., Buschke, A., Reibe, C., Jehmlich, Nico, von Bergen, Martin, Betzel, A., Meyer, B., Rehders, Dirk, Classen, B., Redecke, L., Buschke, A., Reibe, C., Jehmlich, Nico, von Bergen, Martin, Betzel, A., and Meyer, B.

Abstract

Pathogenesis of transmissible spongiform encephalopathies is correlated with a conversion of the normal cellular form of the prion protein (PrPC) into the abnormal isoform (scrapie form of PrP). Contact of the normal PrP with its abnormal isoform, the scrapie form of PrP, induces the transformation. Knowledge of molecules that inhibit such contacts leads to an understanding of the mechanism of the aggregation, and these molecules may serve as leads for drugs against transmissible spongiform encephalopathies. Therefore, we screened a synthetic octapeptide library of the globular domain of the human PrPC for binding affinity to PrPC. Two fragments with binding affinity, 149YYRENMHR156 and 153NMHRYPNQ160, were identified with Kd values of 21 and 25 µM, respectively. A 10-fold excess of peptide 153NMHRYPNQ160 inhibits aggregation of the PrP by 99%. NMR and mass spectrometry showed that the binding region of the peptide 153NMHRYPNQ160 is located at helix 3 of the PrP.

Published
2009

15. Localization of mistletoe lectins ML I-III in Viscum album L. by immunofluorescent and immunogold labeling

Author

Blaschek., W., Gramann, J.-C., and Classen, B.

Subjects
Immunofluorescence -- Usage, Fluorescent antibody technique -- Usage, Lectins -- Identification and classification, Mistletoe -- Health aspects, Biological sciences, Health, Science and technology
Abstract

Purpose: Because of their immunomodulatory and cytotoxic effects mistletoe extracts play an important role as phytotherapeu-tic adjuvants in cancer therapy. Mistletoe lectins ML I-III contribute essentially to the in vivo [...]

Published
2011

29. 41. Interaction of lectin from Viscum album L. with arabinogalactan-proteins from Echinacea purpurea L. Moench

Author

Classen, B., Herbst, B., Blaschek, W., Pfuller, K., and Pfuller, U.

Subjects
Glycoproteins -- Chemical properties -- Research, Plant proteins -- Chemical properties -- Research, Protein-protein interactions -- Research -- Chemical properties, Mistletoe -- Chemical properties -- Research, Echinacea -- Chemical properties -- Research, Biological sciences, Health, Science and technology
Abstract

Today mistletoe extracts play an important role in adjuvant cancer treatment because of their immunostimulating properties. The main active components are mistletoe lectins, ribosome-inactivating proteins of type II consisting of [...]

Published
2007

30. 14. Characterization of arabinogalactan-proteins from Viscum album L. berries and herb

Author

Herbst, B., Classen, B., and Blaschek, W.

Subjects
Glycoproteins -- Identification and classification -- Structure -- Chemical properties -- Nutritional aspects, Plant proteins -- Identification and classification -- Structure -- Chemical properties -- Nutritional aspects, Mistletoe -- Chemical properties -- Nutritional aspects, Biological sciences, Health, Science and technology
Abstract

Arabinogalactan-proteins (AGPs), ubiquitary found in plants, belong to the hydroxyprolin-rich glycoproteins. AGPs are involved in numerous physiological plant processes (Seifert and Roberts, 2007). For AGPs, especially from Echinacea, also different [...]

Published
2007

46. Ultrastructural Observations on the Rust Fungus Puccinia malvacearumin Malva sylvestrisssp. mauritiana

Author

Classen, B., Amelunxen, F., and Blaschek, W.

Abstract

Abstract: Our investigations show the structure of the intracellular mycelium of the rust fungus, Puccinia malvacearum,in leaves of Malva sylvestrisssp. mauritiana.Some intracellular structures of the fungus pass through, others end in the host cells. Fungal infection leads to an increase in the number of mucilage cells in the leaves; but mucilage production does not stop fungal growth. In contrast, the fungus is able to invade mucilage cells without any sign of fungal degradation.

Published
2001
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48. Information transfer in high dependency environments: An ergonomic analysis

Author

Friesdorf, W., Konichezky, S., Groß-Alltag, F., Koller, W., Pollwein, B., Marraro, G., Kari, A., Toro, M., Demeester, M., Nathe, M., Samways, S., Polishuk, I., Müller, I., Bonnaire, A., Schraag, S., and Claßen, B.

Abstract

Abstract: We have studied the information flow in HDE (with special focus on the information transfer process) using data provided by a group of experienced health care professionals. A model of the information flow in HDE was built up. It postulates the existence of quanta of information (due to the artificial fragmentation of the information flow produced by the clinical working processes: organization in shifts, demand of simultaneous activities from different staff members, etc.). This fragmentation is described by using the so-called Clinical Information Process Units (CIPUs), which correspond to patient care activities going on in parallely and serially linked blocks, performed by the staff in the specific environments. Due to a transfer in responsibility over the patient the CIPUs are linked by information transfer events which are described using transfer modules (TraMs). We exemplified 32 CIPUs related to the clinical environments (PreOp, Surgery, Recovery Intensive Care, Ward, Diagnostics, Outpatient) and the health care professional groups (Anesthesiologist/Intensivist, Surgeon, Nurse, Physician, Diagnostic Physician, Physical Therapist). A matrix was established providing the transfer situations among the CIPUs enabling a systematic classification of the TraMs. The contents of the TraMs are built up of information link elements, which are assembled according to the specific settings of the transfer situation given by the emitter, receiver and purpose. In summary we modelled the process of information transfer in HDE through CIPUs, TraMs and information links in a way, which may be useful to design information technology applications or to reorganize the information management in HDE.

Published
1995
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