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1. African army ants at the forefront of virome surveillance in a remote tropical forest

Author

Fritz, Matthieu, Reggiardo, Bérénice, Filloux, Denis, Claude, Lisa, Fernandez, Emmanuel, Mahé, Frédéric, Kraberger, Simona, Custer, Joy M., Becquart, Pierre, Mebaley, Telstar Ndong, Kombila, Linda Bohou, Lenguiya, Léadisaelle H., Boundenga, Larson, Mombo, Illich M., Maganga, Gael D., Niama, Fabien R., Koumba, Jean-Sylvain, Ogliastro, Mylène, Yvon, Michel, Martin, Darren P., Blanc, Stéphane, Varsani, Arvind, Leroy, Eric, and Roumagnac, Philippe

Subjects
Archaeology, CC1-960, Science
Abstract

In this study, we used a predator-enabled metagenomics strategy to sample the virome of a remote and difficult-to-access densely forested African tropical region. Specifically, we focused our study on the use of army ants of the genus Dorylus that are obligate collective foragers and group predators that attack and overwhelm a broad array of animal prey. Using 209 army ant samples collected from 29 colonies and the virion-associated nucleic acid-based metagenomics approach, we showed that a broad diversity of bacterial, plant, invertebrate and vertebrate viral sequences were accumulated by army ants: including sequences from 157 different viral genera in 56 viral families. This suggests that using predators and scavengers such as army ants to sample broad swathes of tropical forest viromes can shed light on the composition and the structure of viral populations of these complex and inaccessible ecosystems.

Published
2023
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2. Patient-Level Pooled Analysis of Endovascular Ultrasound Renal Denervation or a Sham Procedure 6 Months After Medication Escalation: The RADIANCE Clinical Trial Program

Author

Azizi, Michel, Sharp, Andrew S.P., Fisher, Naomi D.L., Weber, Michael A., Lobo, Melvin D., Daemen, Joost, Lurz, Philipp, Mahfoud, Felix, Schmieder, Roland E., Basile, Jan, Bloch, Michael J., Saxena, Manish, Wang, Yale, Sanghvi, Kintur, Jenkins, J. Stephen, Devireddy, Chandan, Rader, Florian, Gosse, Philippe, Claude, Lisa, Augustin, Dimitri A., McClure, Candace K., Kirtane, Ajay J., Wang, Yale, Skeik, Nedaa, Bae, Richard, McMeans, Amy, Goldman, JoAnne, Peterson, Rose, Stephen Jenkins, James, Tutor, Isabelle, Harrison, Michael, Penning, Angel, Devireddy, Chandan, Lea, Janice, Fiebach, Amanda, Merlin, Claudia, Rader, Florian, Dohad, Suhail, Tran, Anne, Bhatia, Kirin, Fisher, Naomi D.L., Sobieszczyk, Piotr, Halliday, Ian, Munson, Tay, Lindsey, Jason, Laster, Steven, Bunte, Mathew, Hart, Anthony, King, Dana, Hall, Jamie, Sanghvi, Kintur, Krathen, Courtney, Lewis, Luot, Willitts, Ashley, Todoran, Thomas, Basile, Jan, Awkar, Anthony, Palmer, Casey, Tecklenburg, Anna, Schindler, John, Pacella, John, Muldoon, Matthew, Albright, MaryJo, Nicholson, Tracy, Flack, John, Chami, Youseff, Hafiz, Abdul Moiz, Starkey, Emily, Adams, Kristal, Bernardo, Nelson, Veis, Judith, Hashim, Hayder, Singh, Suman, Whitman, Donna, Stouffer, Rick, Hinderliter, Alan, Allen, Meghan, Scholl, Tatum, Fong, Pete, Gainer, James, Crook, Sherron, Hatchcock, Ellen, Cohen, Debbie, Giri, Jay, Kobayashi, Taisei, Neubauer, Robin, Naidu, Suveeksha, Kirtane, Ajay J., Radhakrishnan, Jai, Batres, Candido, Edwards, Suzanne, Khuddus, Matheen, Zentko, Suzanne, Touchton, Abby, Roberson, Marti, Bloch, Michael J., Akinapelli, Abhilash, English, Lisa, Neumann, Bridget, Mendelsohn, Farrel, Brantley, Hutton, Cawthon, Thomas, DeRamus, Susan, Wade, Wesley, Fishman, Robert, Tuohy, Edward, LeBlanc, Jessica, McCurry, Tina, Krishnaswamy, Amar, Laffin, Luke, Bajzer, Christopher, Boros, Marilyn, Branche, Monica, Abraham, Josephine, Abraham, Anu, Stijleman, Inge, Hsi, David, Martin, Scott, Portnay, Edward, Fiebach, Maryann, Garavito, Carolina, Adams, Todd, Teklinski, Andrew, Leech, Adam, Drilling, Patrick, Tulik, Lynda, Benzuly, Keith, Paparello, James, Fintel, Dan, Ramirez, Haydee, Kats, Lauren, Huang, Paul, Biswas, Santanu, Risher, Serena, Pratt, Kristina, Ibebuogu, Uzoma, Johnson, Karen, Cushman, William, Jones, Lisa, Jackson, Leigh, Landers, David, Pasala, Tilak, Salazer, Thomas, Canino, Peter, Arakelian, Patricia, Yang, Yi-Ming, Khaliq, Asma, Weinberg, Mitchell, Abetu, Yihenew, Gulliver, Alana, Reilly, J.P., Garasic, Joseph, Chugh, Atul, Bertolet, Barry, Go, Brian, Gallapudi, Raghava, Cohn, Joel, Rogers, Kevin, Saxena, Manish, Mathur, Anthony, Jain, Ajay, Balawon, Armida, Zongo, Oliver, Topham, Christine, Sharp, Andrew, Anderson, Richard, Thompson, Elizabeth, Spiro, Nikki, Hodges, Elizabeth, Holder, Jaqueline, Ellam, Timothy, Bagnall, Alan, Jackson, Ralph, Bridgett, Victoria, Wilson, Peter, Das, Neelanjan, Doulton, Timothy, Loader, David, Hector, Gemma, Levy, Terry, Bent, Clare, Kodoth, Vivek, Horler, Stephanie, Nix, Sara, Robinson, Nicholas, Al-Janabi, Firas, Sayer, Jeremy, Ganesh Iyer, Sudha, Redman, Emily, Ramirez, Jonaifah, Padmanabhan, Sandosh, Sharif, Faisal, Alhmoudi, Aishah, Lunardi, Mattia, Coen, Eileen, Glynn, Nicola, Mahfoud, Felix, Lauder, Lucas, Kulenthiran, Saarraaken, Koch, Christina, Wachter, Angelika, Schmieder, Roland, Schmid, Axel, Kannenkeril, Dennis, Heinritz, Ulrike, Endres-Frohlich, Kerstin, Lurz, Philipp, Rommel, Karl, Fengler, Petzold, Martin, Büttner, Margit, Weil, Joachim, Agdirlioglu, Tolga, Köllner, Tanja, Stephan, Jeannine, Dagkonakis, Nikolaos, Hamann, Frank, Ettl, Ute, Petzsche, Ulrike, Reimer, Peter, Hausberg, Martin, Hinrichs, Ralf, Di Ponio-Voit, Isabella, Lutz, Matthias, Gosse, Philippe, Cremer, Antoine, Papadopoulos, Panteleimon, Gaudissard, Julie, Maire, Florent, Azizi, Michel, Sapoval, Marc, Livrozet, Marine, Regrag, Asma, Paquet, Valerie, Delsart, Pascal, Hennicaux, Justin, Sommeville, Coralie, Bertrand, Fabien, Daemen, Joost, Lafeber, Melvin, Zeijen, Victor, Ruiter, Amo, Huijskens, Elisabeth, van Ramshorst, Jan, Xaplanteris, Panagiotis, Briki, Rachid, de Hemptinne, Quentin, Pascal, Severine, Renard, Katty, Ferdinande, Bert, Iglesias, Juan F., Ehert, Georg, Gallego, Laetitia, Dobretz, Kevin, Bottone, Sylviane, Sanghvi, Kintur, Costello, Josh, Krathan, Courtney, Lewis, Luot, McElvarr, Andrew, Reilly, John, Jenkins, Stephen, Cash, Michael, Williams, Shannon, Jarvis, Maria, Fong, Pete, Laffer, Cheryl, Gainer, James, Robbins, Mark, Crook, Sherron, Maddel, Sarita, Hsi, David, Martin, Scott, Portnay, Edward, Ducey, Maryanne, Rose, Suzanne, DelMastro, Elizabeth, Bangalore, Sripal, Williams, Stephen, Cabos, Stanley, Rodriguez Alvarez, Carolina, Todoran, Thomas, Basile, Jan, Powers, Eric, Hodskins, Emily, Paladugu, Vijay, Tecklenburg, Anna, Devireddy, Chandan, Lea, Janice, Wells, Bryan, Fiebach, Amanda, Merlin, Claudia, Rader, Florian, Dohad, Suhail, Kim, Hyun-Min, Rashid, Mohammad, Abraham, Josephine, Owan, Theophilus, Abraham, Anu, Lavasani, Iran, Neilson, Hailey, Calhoun, David, McElderry, Thomas, Maddox, William, Oparil, Suzanne, Kinder, Sheila, Kirtane, Ajay J., Radhakrishnan, Jai, Batres, Candido, Edwards, Suzanne, Garasic, Joseph, Drachman, Doug, Zusman, Randy, Rosenfield, Kenneth, Do, Danny, Khuddus, Matheen, Zentko, Suzanne, O’Meara, James, Barb, Ilie, Foster, Abby, Boyette, Alice, Wang, Yale, Jay, Desmond, Skeik, Nedaa, Schwartz, Robert, Peterson, Rose, Goldman, Jo Anne, Goldman, Jessie, Ledley, Gary, Katof, Nancy, Potluri, Srinivasa, Biedermann, Scott, Ward, Jacquelyn, White, Megan, Fisher, Naomi D.L., Mauri, Laura, Sobieszczky, Piotr, Smith, Alex, Aseltine, Laura, Stouffer, Rick, Hinderliter, Alan, Pauley, Eric, Wade, Tyrone, Zidar, David, Shishehbor, Mehdi, Effron, Barry, Costa, Marco, Semenec, Terence, Bloch, Michael J., Roongsritong, Chanwit, Nelson, Priscilla, Neumann, Bridget, Cohen, Debbie, Giri, Jay, Neubauer, Robin, Vo, Thu, Chugh, Atul R., Huang, Pei-Hsiu, Jose, Powell, Flack, John, Fishman, Robert, Jones, Michael, Adams, Todd, Bajzer, Christopher, Saxena, Manish, Lobo, Melvin D., Mathur, Anthony, Jain, Ajay, Balawon, Armida, Zongo, Olivier, Levy, Terry, Bent, Clare, Beckett, David, Lakeman, Nicki, Kennard, Sarah, Sharp, Andrew, D’Souza, Richard J., Statton, Sarah, Wilkes, Lindsay, Anning, Christine, Sayer, Jeremy, Ganesh Iyer, Sudha, Robinson, Nicholas, Sevillano, Annaliza, Ocampo, Madelaine, Gerber, Robert, Faris, Mohamad, John Marshall, Andrew, Sinclair, Janet, Pepper, Hayley, Davies, Justin, Chapman, Neil, Burak, Paula, Carvelli, Paula, Jadhav, Sachin, Quinn, Jane, Christian Rump, Lars, Stegbauer, Johannes, Schimmöller, Lars, Potthoff, Sebastian, Schmid, Claudia, Roeder, Sylvia, Weil, Joachim, Hafer, Lukas, Agdirlioglu, Tolga, Köllner, Tanja, Mahfoud, Felix, Böhm, Michael, Ewen, Sebastian, Kulenthiran, Saarraaken, Wachter, Angelika, Koch, Christina, Lurz, Philipp, Fengler, Karl, Rommel, Karl-Philipp, Trautmann, Kai, Petzold, Martin, Schmieder, Roland E., Ott, Christian, Schmid, Axel, Uder, Michael, Heinritz, Ulrike, Fröhlich-Endres, Kerstin, Genth-Zotz, Sabine, Kämpfner, Denise, Grawe, Armin, Höhne, Johannes, Kaesberger, Bärbel, von zur Mühlen, Constantin, Wolf, Dennis, Welzel, Markus, Gosse, Philippe, Cremer, Antoine, Trillaud, Hervé, Papadopoulos, Panteleimon, Maire, Florent, Gaudissard, Julie, Azizi, Michel, Sapoval, Marc, Cornu, Erika, Fouassier, David, Livrozet, Marine, Lorthioir, Aurélien, Paquet, Valérie, Pathak, Atul, Honton, Benjamin, Cottin, Marianne, Petit, Frédéric, Lantelme, Pierre, Berge, Constance, Courand, Pierre-Yves, Langevin, Fatou, Delsart, Pascal, Longere, Benjamin, Ledieu, Guillaume, Pontana, François, Sommeville, Coralie, Bertrand, Fabien, Daemen, Joost, Feyz, Lida, Zeijen, Victor, Ruiter, Arno, Huyskens, Elisabeth, Blankestijn, Peter, Voskuil, Michiel, Rittersma, Zwaantina, Dolmans, Helma, Kroon, A.A., van Zwam, W.H., Vranken, Jeannique, de Haan, Claudia, Persu, Alexandre, Renkin, Jean, Maes, Frédéric, Beauloye, Christophe, Lengelé, Jean-Philippe, Huyberechts, Dominique, Bouvier, Anne, Witkowski, Adam, Januszewicz, Andrzej, Kądziela, Jacek, Prejbisj, Aleksander, Hering, Dagmara, Ciecwierz, Dariusz, Jaguszewski, Milosz J., Owczuk, Radoslaw, Ciecwierz, Dariusz, Jaguszewski, Milosz J., Wang, Yale, Jay, Desmond, Skeik, Nedaa, Schwartz, Robert, Rader, Florian, Dohad, Suhail, Victor, Ronald, Sanghvi, Kintur, Costello, Josh, Walsh, Courtney, Abraham, Josephine, Owan, Theophilus, Abraham, Anu, Fisher, Naomi D.L., Mauri, Laura, Sobieszczky, Piotr, Williams, Jonathan, Bloch, Michael J., Roongsritong, Chanwit, Todoran, Thomas, Basile, Jan, Powers, Eric, Hodskins, Emily, Fong, Pete, Laffer, Cheryl, Gainer, James, Robbins, Mark, Reilly, John, Cash, Michael, Goldman, Jessie, Aggarwal, Sandeep, Ledley, Gary, Hsi, David, Martin, Scott, Portnay, Edward, Calhoun, David, McElderry, Thomas, Maddox, William, Oparil, Suzanne, Huang, Pei-Hsiu, Jose, Powell, Khuddus, Matheen, Zentko, Suzanne, O’Meara, James, Barb, Ilie, Garasic, Joseph, Drachman, Doug, Zusman, Randy, Rosenfield, Kenneth, Devireddy, Chandan, Lea, Janice, Wells, Bryan, Stouffer, Rick, Hinderliter, Alan, Pauley, Eric, Potluri, Srinivasa, Biedermann, Scott, Bangalore, Sripal, Williams, Stephen, Zidar, David, Shishehbor, Mehdi, Effron, Barry, Costa, Marco, Kirtane, Ajay J., Radhakrishnan, Jai, Lobo, Melvin D., Mathur, Anthony, Jain, Ajay, Sayer, Jeremy, Ganesh Iyer, Sudha, Robinson, Nicholas, Ali Edroos, Sadat, Levy, Terry, Patel, Amit, Beckett, David, Bent, Clare, Davies, Justin, Chapman, Neil, Shun Shin, Matthew, Howard, James, Sharp, Andrew S.P., Joseph, Anil, D’Souza, Richard, Gerber, Robert, Faris, Mohamad, John Marshall, Andrew, Elorz, Cristina, Lurz, Philipp, Höllriegel, Robert, Fengler, Karl, Rommel, Karl-Philipp, Mahfoud, Felix, Böhm, Michael, Ewen, Sebastian, Lucic, Jelena, Schmieder, Roland E., Ott, Christian, Schmid, Axel, Uder, Michael, Rump, Christian, Stegbauer, Johannes, Kröpil, Patric, Azizi, Michel, Sapoval, Marc, Cornu, Erika, Fouassier, David, Gosse, Philippe, Cremer, Antoine, Trillaud, Hervé, Papadopoulos, Panteleimon, Pathak, Atul, Honton, Benjamin, Lantelme, Pierre, Berge, Constance, Courand, Pierre-Yves, Daemen, Joost, Feyz, Lida, Blankestijn, Peter, Voskuil, Michiel, Rittersma, Zwaantina, Kroon, A.A., van Zwam, W.H., Persu, Alexandre, and Renkin, Jean

Published
2024
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3. Predictors of blood pressure response to ultrasound renal denervation in the RADIANCE-HTN SOLO study

Author

Saxena, Manish, Schmieder, Roland E., Kirtane, Ajay J., Mahfoud, Felix, Daemen, Joost, Basile, Jan, Lurz, Philipp, Gosse, Philippe, Sanghvi, Kintur, Fisher, Naomi D. L., Rump, Lars C., Pathak, Atul, Blankestijn, Peter J., Mathur, Anthony, Wang, Yale, Weber, Michael A., Sharp, Andrew S. P., Bloch, Michael J., Barman, Neil C., Claude, Lisa, Song, Yang, Azizi, Michel, and Lobo, Melvin D.

Published
2022
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5. 12-Month Results From the Unblinded Phase of the RADIANCE-HTN SOLO Trial of Ultrasound Renal Denervation

Author

Chertow, Glenn, Kahan, Thomas, Dauerman, Harold, Ullery, Steven, Azizi, Michel, Daemen, Joost, Lobo, Melvin D., Mahfoud, Felix, Sharp, Andrew S.P., Schmieder, Roland E., Wang, Yale, Saxena, Manish, Lurz, Philipp, Sayer, Jeremy, Bloch, Michael J., Basile, Jan, Weber, Michael A., Rump, Lars C., Levy, Terry, Sapoval, Marc, Sanghvi, Kintur, Rader, Florian, Fisher, Naomi D.L., Gosse, Philippe, Abraham, Josephine, Claude, Lisa, Barman, Neil C., McClure, Candace K., Liu, Yuyin, and Kirtane, Ajay J.

Published
2020
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7. Patient-Level Pooled Analysis of Endovascular Ultrasound Renal Denervation or a Sham Procedure at 6 Months Following Medication Escalation: The RADIANCE Clinical Trial Program

Author

Azizi, Michel, primary, Sharp, Andrew S.P., additional, Fisher, Naomi D.L., additional, Weber, Michael A., additional, Lobo, Melvin D., additional, Daemen, Joost, additional, Lurz, Philipp, additional, Mahfoud, Felix, additional, Schmieder, Roland E., additional, Basile, Jan, additional, Bloch, Michael J., additional, Saxena, Manish, additional, Wang, Yale, additional, Sanghvi, Kintur, additional, Jenkins, J. Stephen, additional, Devireddy, Chandan, additional, Rader, Florian, additional, Gosse, Philippe, additional, Claude, Lisa, additional, Augustin, Dimitri A., additional, McClure, Candace K., additional, and Kirtane, Ajay J., additional

Published
2023
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9. Virome release of an invasive exotic plant species in Southern France

Author

Moubset, Oumaima, Filloux, Denis, Fontes, Hugo, Julian, Charlotte, Fernandez, Emmanuel, Claude, Lisa, Galzi, Serge, Chiroleu, Frédéric, Mesleard, François, Kraberger, Simona, Custer, Joy M., Salywon, Andrew M., Makings, Elizabeth, Martin Patrick, Darren, Varsani, Arvind, Ravigne, Virginie, Roumagnac, Philippe, Moubset, Oumaima, Filloux, Denis, Fontes, Hugo, Julian, Charlotte, Fernandez, Emmanuel, Claude, Lisa, Galzi, Serge, Chiroleu, Frédéric, Mesleard, François, Kraberger, Simona, Custer, Joy M., Salywon, Andrew M., Makings, Elizabeth, Martin Patrick, Darren, Varsani, Arvind, Ravigne, Virginie, and Roumagnac, Philippe

Abstract

The increase in human-mediated introduction of plant species to new regions due to global travel and globalization has resulted in a rise of invasive exotic plants (IEPs) that can have significant effects on biodiversity, ecosystem processes, and food production. The introduction of IEPs to new regions often occurs through seed dispersal, and most pathogens are not vertically transmitted, leading to low viral loads in these plants. Also, most pathogens are not evenly distributed across the Earth, meaning that IEPs colonizing a new territory are unlikely to encounter pathogens from their native range. This situation, referred to as the "enemy-release hypothesis" suggests that decreased pathogen-mediated selective pressures on IEPs in colonized territories will result in increased IEP populations, densities, and geographical distributions. To test the enemy-release hypothesis, the virome of an invasive cane bluestem (Bothriochloa barbinodis) was compared to that of four or more indigenous grass species in both naturalized and native ranges. The results showed that the IEPs had lower viral infection loads than the native grasses, providing evidence that supports the enemy release hypothesis. Novel viruses associated with Bothriochloa barbinodis were further partially or fully sequenced and the phylogenetic relationships of these viral sequences and representative sequences of corresponding virus families were analyzed.

Published
2023

10. from boots on the ground to nucleotides in the sequencer: Advances in the study of plant virus ecology using plant virus metagenomics

Author

Fritz, Matthieu, Reggiardo, Bérénice, Filloux, Denis, Claude, Lisa, Fernandez, Emmanuel, Mahe, Frédéric, Kraberger, Simona, Custer, Joy M., Becquart, Pierre, Ndong Mebaley, Telstar, Bohou Kombila, Linda, Lenguiya, Léadisaelle H., Boundenga, Larson, Mombo, Illich M., Maganda, Gael D., Niama, Fabien R., Koumba, Jean-Sylvain, Ogliastro, Mylène, Yvon, Michel, Martin, Darren Patrick, Blanc, Stéphane, Varsani, Arvind, Leroy, Eric M., Roumagnac, Philippe, Fritz, Matthieu, Reggiardo, Bérénice, Filloux, Denis, Claude, Lisa, Fernandez, Emmanuel, Mahe, Frédéric, Kraberger, Simona, Custer, Joy M., Becquart, Pierre, Ndong Mebaley, Telstar, Bohou Kombila, Linda, Lenguiya, Léadisaelle H., Boundenga, Larson, Mombo, Illich M., Maganda, Gael D., Niama, Fabien R., Koumba, Jean-Sylvain, Ogliastro, Mylène, Yvon, Michel, Martin, Darren Patrick, Blanc, Stéphane, Varsani, Arvind, Leroy, Eric M., and Roumagnac, Philippe

Abstract

Plant virus ecology began to be explored at the end of the 19th century. Since then, major advances have revealed complex virus–host–vector interactions in a variety of environments. These advances have been accelerated by development of new technologies for virus detection and characterization, the latest of which being high-throughput sequencing (HTS). HTS technologies have proved to be effective for non-targeted characterization of all or nearly all viruses present in a sample without requiring prior information about virus identity, as would be needed for virus-targeted tests. Plant virus metagenomics studies have thus made important advances, including characterization of the complex interactions between phytovirus dynamics and the structure of multi-species host communities, and documentation of the effects of anthropogenic ecosystem simplification on plant virus emergence and diversity. However, such studies must overcome challenges at every stage, from plant sampling to bioinformatics analysis. Results of two recent studies will be presented. While the first study aimed at systematically evaluate plant-associated viromes across broad agroecological interfaces, the second study aimed at using a predator-enabled metagenomics strategy to sample the virome of a remote and difficult to access densely forested African tropical region.

Published
2023

11. Once upon a time, the VANA story based on the review 'virion-associated nucleic acid-based metagenomics : a decade of advances in molecular characterization of plant viruses'

Author

Moubset, Oumaima, François, Sarah, Maclot, François, Palanga, Essowé, Julian, Charlotte, Claude, Lisa, Fernandez, Emmanuel, Rott, Philippe, Daugrois, Jean-Heinrich, Antoine-Lorquin, Aymeric, Bernardo, Pauline, Blouin, Arnaud G., Temple, Coline, Kraberger, Simona, Fontenele, Rafaela S, Harkins, Gordon William, Ma, Yuxin, Marais, Armelle, Candresse, Thierry, Ben Chehida, Sélim, Lefeuvre, Pierre, Lett, Jean-Michel, Varsani, Arvind, Massart, Sébastien, Ogliastro, Mylène, Martin, Darren Patrick, Filloux, Denis, Roumagnac, Philippe, Moubset, Oumaima, François, Sarah, Maclot, François, Palanga, Essowé, Julian, Charlotte, Claude, Lisa, Fernandez, Emmanuel, Rott, Philippe, Daugrois, Jean-Heinrich, Antoine-Lorquin, Aymeric, Bernardo, Pauline, Blouin, Arnaud G., Temple, Coline, Kraberger, Simona, Fontenele, Rafaela S, Harkins, Gordon William, Ma, Yuxin, Marais, Armelle, Candresse, Thierry, Ben Chehida, Sélim, Lefeuvre, Pierre, Lett, Jean-Michel, Varsani, Arvind, Massart, Sébastien, Ogliastro, Mylène, Martin, Darren Patrick, Filloux, Denis, and Roumagnac, Philippe

Abstract

Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 25 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2022). Collectively, viruses from 94 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided.

Published
2023

12. Sugarcane mild mosaic virus recognized as a new ampelovirus species infecting sugarcane

Author

Mollov, Dimitre S., Filloux, Denis, Grinstead, Samuel, Bolus, Stephen, Claude, Lisa, Fernandez, Emmanuel, Julian, Charlotte, Candresse, Thierry, Lockhart, Benham E.L., Roumagnac, Philippe, Rott, Philippe, Daugrois, Jean-Heinrich, Mollov, Dimitre S., Filloux, Denis, Grinstead, Samuel, Bolus, Stephen, Claude, Lisa, Fernandez, Emmanuel, Julian, Charlotte, Candresse, Thierry, Lockhart, Benham E.L., Roumagnac, Philippe, Rott, Philippe, and Daugrois, Jean-Heinrich

Abstract

The complete genome sequence of a previously uncharacterized sugarcane-infecting virus of the genus Ampelovirus was obtained by high-throughput sequencing (HTS) and rapid amplification of cDNA ends. This genome consisted of 13,114 nucleotides (nt) and harboured seven open reading frames. Subsequently, 15 additional complete or near-complete genome sequences of this virus were obtained by HTS from different sugarcane varieties originating in Africa, Asia, North and South America. Phylogenetic and pairwise identity analyses with all these sequences revealed that they belonged to a new Ampelovirus species, family Closteroviridae. The 16 virus isolates were distributed in three phylogenetic groups and potential recombinant events were identified for only one of these isolates. An immunocapture-reverse transcription PCR (IC-RT-PCR) assay was developed with antibodies previously produced against a clostero-like virus that had not yet been recognized by the International Committee on Taxonomy of Viruses but for which the name sugarcane mild mosaic virus (SCMMV) had been proposed. The new ampelovirus was detected by IC-RT-PCR, and Sanger-sequenced amplicons confirmed the identity of the virus based on HTS data. The complete or near-complete sequences reported here represent the first genomic sequences for SCMMV that has a wide geographical distribution. Furthermore, the newly developed RT-PCR diagnostic assay will facilitate the understanding of SCMMV epidemiology and will help improve management and quarantine diagnostic.

Published
2023

14. African army ants at the forefront of virome surveillance in a remote tropical forest

Author

Fritz, Matthieu, primary, Reggiardo, Bérénice, additional, Filloux, Denis, additional, Claude, Lisa, additional, Fernandez, Emmanuel, additional, Mahé, Frédéric, additional, Kraberger, Simona, additional, Custer, Joy M., additional, Becquart, Pierre, additional, Mebaley, Telstar Ndong, additional, Kombila, Linda Bohou, additional, Lenguiya, Léadisaelle H., additional, Boundenga, Larson, additional, Mombo, Illich M., additional, Maganga, Gael D., additional, Niama, Fabien R., additional, Koumba, Jean-Sylvain, additional, Ogliastro, Mylène, additional, Yvon, Michel, additional, Martin, Darren P., additional, Blanc, Stéphane, additional, Varsani, Arvind, additional, Leroy, Eric, additional, and Roumagnac, Philippe, additional

Published
2022
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15. Endovascular Ultrasound Renal Denervation to Treat Hypertension: The RADIANCE II Randomized Clinical Trial

Author

Azizi, Michel, Saxena, Manish, Wang, Yale, Jenkins, J. Stephen, Devireddy, Chandan, Rader, Florian, Fisher, Naomi D.L., Schmieder, Roland E., Mahfoud, Felix, Lindsey, Jason, Sanghvi, Kintur, Todoran, Thomas M., Pacella, John, Flack, John, Daemen, Joost, Sharp, Andrew S.P., Lurz, Philipp, Bloch, Michael J., Weber, Michael A., Lobo, Melvin D., Basile, Jan, Claude, Lisa, Reeve-Stoffer, Helen, McClure, Candace K., Kirtane, Ajay J., and Cardiology

Abstract

IMPORTANCE: Two initial sham-controlled trials demonstrated that ultrasound renal denervation decreases blood pressure (BP) in patients with mild to moderate hypertension and hypertension that is resistant to treatment. OBJECTIVE: To study the efficacy and safety of ultrasound renal denervation without the confounding influence of antihypertensive medications in patients with hypertension. DESIGN, SETTING, AND PARTICIPANTS: Sham-controlled, randomized clinical trial with patients and outcome assessors blinded to treatment assignment that was conducted between January 14, 2019, and March 25, 2022, at 37 centers in the US and 24 centers in Europe, with randomization stratified by center. Patients aged 18 years to 75 years with hypertension (seated office systolic BP [SBP] ≥140 mm Hg and diastolic BP [DBP] ≥90 mm Hg despite taking up to 2 antihypertensive medications) were eligible if they had an ambulatory SBP/DBP of 135/85 mm Hg or greater and an SBP/DBP less than 170/105 mm Hg after a 4-week washout of their medications. Patients with an estimated glomerular filtration rate of 40 mL/min/1.73 m2 or greater and with suitable renal artery anatomy were randomized 2:1 to undergo ultrasound renal denervation or a sham procedure. Patients were to abstain from antihypertensive medications until the 2-month follow-up unless prespecified BP criteria were exceeded and were associated with clinical symptoms. INTERVENTIONS: Ultrasound renal denervation vs a sham procedure. MAIN OUTCOMES AND MEASURES: The primary efficacy outcome was the mean change in daytime ambulatory SBP at 2 months. The primary safety composite outcome of major adverse events included death, kidney failure, and major embolic, vascular, cardiovascular, cerebrovascular, and hypertensive events at 30 days and renal artery stenosis greater than 70% detected at 6 months. The secondary outcomes included mean change in 24-hour ambulatory SBP, home SBP, office SBP, and all DBP parameters at 2 months. RESULTS: Among 1038 eligible patients, 150 were randomized to ultrasound renal denervation and 74 to a sham procedure (mean age, 55 years [SD, 9.3 years]; 28.6% female; and 16.1% self-identified as Black or African American). The reduction in daytime ambulatory SBP was greater with ultrasound renal denervation (mean, -7.9 mm Hg [SD, 11.6 mm Hg]) vs the sham procedure (mean, -1.8 mm Hg [SD, 9.5 mm Hg]) (baseline-adjusted between-group difference, -6.3 mm Hg [95% CI, -9.3 to -3.2 mm Hg], P

Published
2023

16. Virion-associated nucleic acid-based metagenomics: a decade of advances in molecular characterization of plant viruses

Author

Moubset, Oumaima, François, Sarah, Maclot, François, Palanga, Essowé, Julian, Charlotte, Claude, Lisa, Fernandez, Emmanuel, Rott, Philippe, Daugrois, Jean-Heinrich, Antoine-Lorquin, Aymeric, Bernardo, Pauline, Blouin, Arnaud G., Temple, Coline, Kraberger, Simona, Fontenele, Rafaela S, Harkins, Gordon William, Ma, Yuxin, Marais, Armelle, Candresse, Thierry, Ben Chehida, Sélim, Lefeuvre, Pierre, Lett, Jean-Michel, Varsani, Arvind, Massart, Sébastien, Ogliastro, Mylène, Martin, Darren Patrick, Filloux, Denis, Roumagnac, Philippe, Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), University of Oxford, Gembloux Agro-Bio Tech [Gembloux], Université de Liège, Institut Togolais de Recherche Agronomique (ITRA), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM), Enza Zaden, Agroscope, Arizona State University [Tempe] (ASU), University of the Western Cape (UWC), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Cape Town, European Union (ERDF, contract GURDT I2016-1731-0006632), Conseil Régional de La Réunion, project SEVIPLANT from Federal Public 17 Service, Public Health, Belgium, Grant Agreement no. RT 18/3, ANR-19-CE35-0008,PHYTOVIRUS,Mesure et cartographie de la richesse des virus des plante à l'échelle de l'écosytème(2019), and European Project: 813542,INEXTVIR

Subjects
Identification, Ecology, viruses, [SDV]Life Sciences [q-bio], Virion, Virus des végétaux, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Plants, génomique, Plant Viruses, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Nucleic Acids, Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Metagenomics, Pathogen Detection, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Ecosystem, H20 - Maladies des plantes, Plant Diseases
Abstract

Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 24 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2020). Collectively, viruses from 85 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Published
2022

17. Effects of Renal Denervation vs Sham in Resistant Hypertension After Medication Escalation

Author

Azizi, Michel, Mahfoud, Felix, Weber, Michael, Sharp, Andrew, Schmieder, Roland, Lurz, Philipp, Lobo, Melvin, Fisher, Naomi, Daemen, Joost, Bloch, Michael, Basile, Jan, Sanghvi, Kintur, Saxena, Manish, Gosse, Philippe, Jenkins, J. Stephen, Levy, Terry, Persu, Alexandre, Kably, Benjamin, Claude, Lisa, Reeve-Stoffer, Helen, Mcclure, Candace, Kirtane, Ajay, Mullin, Christopher, Thackeray, Lisa, Chertow, Glenn, Kahan, Thomas, Dauerman, Harold, Ullery, Steven, Abbott, J. Dawn, Loening, Andreas, Zagoria, Ron, Costello, John, Krathan, Courtney, Lewis, Luot, Mcelvarr, Andrew, Reilly, John, Cash, Michael, Williams, Shannon, Jarvis, Maria, Fong, Pete, Laffer, Cheryl, Gainer, James, Robbins, Mark, Crook, Sherron, Maddel, Sarita, Hsi, David, Martin, Scott, Portnay, Edward, Ducey, Maryanne, Rose, Suzanne, Delmastro, Elizabeth, Bangalore, Sripal, Williams, Stephen, Cabos, Stanley, Rodriguez Alvarez, Carolina, Todoran, Thomas, Powers, Eric, Hodskins, Emily, Paladugu, Vijay, Tecklenburg, Anna, Devireddy, Chandan, Lea, Janice, Wells, Bryan, Fiebach, Amanda, Merlin, Claudia, Rader, Florian, Dohad, Suhail, Kim, Hyun-Min, Rashid, Mohammad, Abraham, Josephine, Owan, Theophilus, Abraham, Anu, Lavasani, Iran, Neilson, Hailey, Calhoun, David, Mcelderry, Thomas, Maddox, William, Oparil, Suzanne, Kinder, Sheila, Radhakrishnan, Jai, Batres, Candido, Edwards, Suzanne, Garasic, Joseph, Drachman, Doug, Zusman, Randy, Rosenfield, Kenneth, Do, Danny, Khuddus, Matheen, Zentko, Suzanne, O'Meara, James, Barb, Ilie, Foster, Abby, Boyette, Alice, Wang, Yale, Jay, Desmond, Skeik, Nedaa, Schwartz, Robert, Peterson, Rose, Goldman, Jo Anne, Goldman, Jessie, Ledley, Gary, Katof, Nancy, Potluri, Srinivasa, Biedermann, Scott, Ward, Jacquelyn, White, Megan, Mauri, Laura, Sobieszczky, Piotr, Smith, Alex, Aseltine, Laura, Stouffer, Rick, Hinderliter, Alan, Pauley, Eric, Wade, Tyrone, Zidar, David, Shishehbor, Mehdi, Effron, Barry, Costa, Marco, Semenec, Terence, Roongsritong, Chanwit, Nelson, Priscilla, Neumann, Bridget, Cohen, Debbie, Giri, Jay, Neubauer, Robin, Vo, Thu, Chugh, Atul, Huang, Pei-Hsiu, Jose, Powell, Flack, John, Fishman, Robert, Jones, Michael, Adams, Todd, Bajzer, Christopher, Mathur, Anthony, Jain, Ajay, Balawon, Armida, Zongo, Olivier, Bent, Clare, Beckett, David, Lakeman, Nicki, Kennard, Sarah, D’souza, Richard, Statton, Sarah, Wilkes, Lindsay, Anning, Christine, Sayer, Jeremy, Iyer, Sudha Ganesh, Robinson, Nicholas, Sevillano, Annaliza, Ocampo, Madelaine, Gerber, Robert, Faris, Mohamad, Marshall, Andrew John, Sinclair, Janet, Pepper, Hayley, Davies, Justin, Chapman, Neil, Burak, Paula, Carvelli, Paula, Jadhav, Sachin, Quinn, Jane, Rump, Lars Christian, Stegbauer, Johannes, Schimmöller, Lars, Potthoff, Sebastian, Schmid, Claudia, Roeder, Sylvia, Weil, Joachim, Hafer, Lukas, Agdirlioglu, Tolga, Köllner, Tanja, Böhm, Michael, Ewen, Sebastian, Kulenthiran, Saarraaken, Wachter, Angelika, Koch, Christina, Fengler, Karl, Rommel, Karl-Philipp, Trautmann, Kai, Petzold, Martin, Ott, Christian, Schmid, Axel, Uder, Michael, Heinritz, Ulrike, Fröhlich-Endres, Kerstin, Genth-Zotz, Sabine, Kämpfner, Denise, Grawe, Armin, Höhne, Johannes, Kaesberger, Bärbel, von Zur Mühlen, Constantin, Wolf, Dennis, Welzel, Markus, Heinrichs, Gudrun, Trabitzsch, Barbara, Cremer, Antoine, Trillaud, Hervé, Papadopoulos, Panteleimon, Maire, Florent, Gaudissard, Julie, Sapoval, Marc, Livrozet, Marine, Lorthioir, Aurélien, Amar, Laurence, Paquet, Valérie, Pathak, Atul, Honton, Benjamin, Cottin, Marianne, Petit, Frédéric, Lantelme, Pierre, Berge, Constance, Courand, Pierre-Yves, Langevin, Fatou, Delsart, Pascal, Longere, Benjamin, Ledieu, Guillaume, Pontana, François, Sommeville, Coralie, Bertrand, Fabien, Feyz, Lida, Zeijen, Victor, Ruiter, Arno, Huysken, Elisabeth, Blankestijn, Peter, Voskuil, Michiel, Rittersma, Zwaantina, Dolmans, Helma, Kroon, A.A., van Zwam, W.H., Vranken, Jeannique, de Haan., Claudia, Renkin, Jean, Maes, Frédéric, Beauloye, Christophe, Lengelé, Jean-Philippe, Huyberechts, Dominique, Bouvie, Anne, Witkowski, Adam, Januszewicz, Andrzej, Kądziela, Jacek, Prejbisj, Aleksander, Hering, Dagmara, Ciecwierz, Dariusz, Jaguszewski, Milosz, Owczuk, Radoslaw, CIC - HEGP (CIC 1418), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), CArdiovasculaire Rénal Transplantation nEurovasculaire [Paris] (DMU CARTE), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Saarland University [Saarbrücken], Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS)-Centre National de la Recherche Scientifique (CNRS), University Hospital of Wales (UHW), University of Exeter, Universitätsklinikum Erlangen [Erlangen], Leipzig University, Queen Mary University of London (QMUL), Brigham & Women’s Hospital [Boston] (BWH), Harvard Medical School [Boston] (HMS), Erasmus University Medical Center [Rotterdam] (Erasmus MC), University of Nevada [Reno], Medical University of South Carolina [Charleston] (MUSC), Hôpital Saint-André, Royal Bournemouth Hospital, Université Catholique de Louvain = Catholic University of Louvain (UCL), Cliniques Universitaires Saint-Luc [Bruxelles], Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Columbia University Medical Center (CUMC), Columbia University [New York], Danderyds sjukhus = Danderyd University Hospital, University of Vermont [Burlington], Stanford University, Argolyn Bioscience, Inc., and Argolyn Bioscience

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Auteurs : RADIANCE-HTN Investigators; International audience; Importance Although early trials of endovascular renal denervation (RDN) for patients with resistant hypertension (RHTN) reported inconsistent results, ultrasound RDN (uRDN) was found to decrease blood pressure (BP) vs sham at 2 months in patients with RHTN taking stable background medications in the Study of the ReCor Medical Paradise System in Clinical Hypertension (RADIANCE-HTN TRIO) trial. Objectives To report the prespecified analysis of the persistence of the BP effects and safety of uRDN vs sham at 6 months in conjunction with escalating antihypertensive medications. Design, Setting, and Participants This randomized, sham-controlled, clinical trial with outcome assessors and patients blinded to treatment assignment, enrolled patients from March 11, 2016, to March 13, 2020. This was an international, multicenter study conducted in the US and Europe. Participants with daytime ambulatory BP of 135/85 mm Hg or higher after 4 weeks of single-pill triple-combination treatment (angiotensin-receptor blocker, calcium channel blocker, and thiazide diuretic) with estimated glomerular filtration rate (eGFR) of 40 mL/min/1.73 m 2 or greater were randomly assigned to uRDN or sham with medications unchanged through 2 months. From 2 to 5 months, if monthly home BP was 135/85 mm Hg or higher, standardized stepped-care antihypertensive treatment starting with aldosterone antagonists was initiated under blinding to treatment assignment. Interventions uRDN vs sham procedure in conjunction with added medications to target BP control. Main Outcomes and Measures Six-month change in medications, change in daytime ambulatory systolic BP, change in home systolic BP adjusted for baseline BP and medications, and safety. Results A total of 65 of 69 participants in the uRDN group and 64 of 67 participants in the sham group (mean [SD] age, 52.4 [8.3] years; 104 male [80.6%]) with a mean (SD) eGFR of 81.5 (22.8) mL/min/1.73 m 2 had 6-month daytime ambulatory BP measurements. Fewer medications were added in the uRDN group (mean [SD], 0.7 [1.0] medications) vs sham (mean [SD], 1.1 [1.1] medications; P = .045) and fewer patients in the uRDN group received aldosterone antagonists at 6 months (26 of 65 [40.0%] vs 39 of 64 [60.9%]; P = .02). Despite less intensive standardized stepped-care antihypertensive treatment, mean (SD) daytime ambulatory BP at 6 months was 138.3 (15.1) mm Hg with uRDN vs 139.0 (14.3) mm Hg with sham (additional decreases of −2.4 [16.6] vs −7.0 [16.7] mm Hg from month 2, respectively), whereas home SBP was lowered to a greater extent with uRDN by 4.3 mm Hg (95% CI, 0.5-8.1 mm Hg; P = .03) in a mixed model adjusting for baseline and number of medications. Adverse events were infrequent and similar between groups. Conclusions and Relevance In this study, in patients with RHTN initially randomly assigned to uRDN or a sham procedure and who had persistent elevation of BP at 2 months after the procedure, standardized stepped-care antihypertensive treatment escalation resulted in similar BP reduction in both groups at 6 months, with fewer additional medications required in the uRDN group. Trial Registration ClinicalTrials.gov Identifier: NCT02649426

Published
2022

18. Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus

Author

Mollov, Dimitre, primary, Filloux, Denis, additional, Grinstead, Samuel, additional, Bolus, Stephen, additional, Claude, Lisa, additional, Fernandez, Emanuel, additional, Julian, Charlotte, additional, Candresse, Thierry, additional, Lockhart, Ben, additional, Roumagnac, Philippe, additional, Rott, Philippe, additional, and Daugrois, Jean Heinrich, additional

Published
2022
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19. Patient-Level Pooled Analysis of Ultrasound Renal Denervation in the Sham-Controlled RADIANCE II, RADIANCE-HTN SOLO, and RADIANCE-HTN TRIO Trials

Author

Kirtane, Ajay J, Sharp, Andrew S P, Mahfoud, Felix, Fisher, Naomi D L, Schmieder, Roland E, Daemen, Joost, Lobo, Melvin D, Lurz, Philipp, Basile, Jan, Bloch, Michael J, Weber, Michael A, Saxena, Manish, Wang, Yale, Sanghvi, Kintur, Jenkins, J Stephen, Devireddy, Chandan, Rader, Florian, Gosse, Philippe, Sapoval, Marc, Barman, Neil C, Claude, Lisa, Augustin, Dimitri, Thackeray, Lisa, Mullin, Christopher M, Azizi, Michel, and Cardiology

Subjects
Cardiology and Cardiovascular Medicine
Abstract

ImportanceUltrasound renal denervation (uRDN) was shown to lower blood pressure (BP) in patients with uncontrolled hypertension (HTN). Establishing the magnitude and consistency of the uRDN effect across the HTN spectrum is clinically important.ObjectiveTo characterize the effectiveness and safety of uRDN vs a sham procedure from individual patient-level pooled data across uRDN trials including either patients with mild to moderate HTN on a background of no medications or with HTN resistant to standardized triple-combination therapy.Data SourcesA Study of the ReCor Medical Paradise System in Clinical Hypertension (RADIANCE-HTN SOLO and TRIO) and A Study of the ReCor Medical Paradise System in Stage II Hypertension (RADIANCE II) trials.Study SelectionTrials with similar designs, standardized operational implementation (medication standardization and blinding of both patients and physicians to treatment assignment), and follow-up.Data Extraction and SynthesisPooled analysis using individual patient-level data using linear regression models to compare uRDN with sham across the trials.Main Outcomes and MeasuresThe primary outcome was baseline-adjusted change in 2-month daytime ambulatory systolic BP (dASBP) between groups.ResultsA total of 506 patients were randomized in the 3 studies (uRDN, 293; sham, 213; mean [SD] age, 54.1 [9.3]; 354 male [70.0%]). After a 1-month medication stabilization period, dASBP was similar between the groups (mean [SD], uRDN, 150.3 [9.2] mm Hg; sham, 150.8 [10.5] mm Hg). At 2 months, dASBP decreased by 8.5 mm Hg to mean (SD) 141.8 (13.8) mm Hg among patients treated with uRDN and by 2.9 mm Hg to 147.9 (14.6) mm Hg among patients treated with a sham procedure (mean difference, −5.9; 95% CI, −8.1 to −3.8 mm Hg; P Conclusions and RelevanceResults of this patient-level pooled analysis suggest that BP reductions with uRDN were consistent across HTN severity in sham-controlled trials designed with a 2-month primary end point to standardize medications across randomized groups.Trial RegistrationClinicalTrials.gov Identifier: NCT02649426 and NCT03614260

Published
2023

20. Effects of Renal Denervation vs Sham in Resistant Hypertension after Medication Escalation:Prespecified Analysis at 6 Months of the RADIANCE-HTN TRIO Randomized Clinical Trial

Author

Azizi, Michel, Mahfoud, Felix, Weber, Michael A., Sharp, Andrew S.P., Schmieder, Roland E., Lurz, Philipp, Lobo, Melvin D., Fisher, Naomi D.L., Daemen, Joost, Bloch, Michael J., Basile, Jan, Sanghvi, Kintur, Saxena, Manish, Gosse, Philippe, Jenkins, J. Stephen, Levy, Terry, Persu, Alexandre, Kably, Benjamin, Claude, Lisa, Reeve-Stoffer, Helen, McClure, Candace, Kirtane, Ajay J., Azizi, Michel, Mahfoud, Felix, Weber, Michael A., Sharp, Andrew S.P., Schmieder, Roland E., Lurz, Philipp, Lobo, Melvin D., Fisher, Naomi D.L., Daemen, Joost, Bloch, Michael J., Basile, Jan, Sanghvi, Kintur, Saxena, Manish, Gosse, Philippe, Jenkins, J. Stephen, Levy, Terry, Persu, Alexandre, Kably, Benjamin, Claude, Lisa, Reeve-Stoffer, Helen, McClure, Candace, and Kirtane, Ajay J.

Abstract

Importance: Although early trials of endovascular renal denervation (RDN) for patients with resistant hypertension (RHTN) reported inconsistent results, ultrasound RDN (uRDN) was found to decrease blood pressure (BP) vs sham at 2 months in patients with RHTN taking stable background medications in the Study of the ReCor Medical Paradise System in Clinical Hypertension (RADIANCE-HTN TRIO) trial. Objectives: To report the prespecified analysis of the persistence of the BP effects and safety of uRDN vs sham at 6 months in conjunction with escalating antihypertensive medications. Design, Setting, and Participants: This randomized, sham-controlled, clinical trial with outcome assessors and patients blinded to treatment assignment, enrolled patients from March 11, 2016, to March 13, 2020. This was an international, multicenter study conducted in the US and Europe. Participants with daytime ambulatory BP of 135/85 mm Hg or higher after 4 weeks of single-pill triple-combination treatment (angiotensin-receptor blocker, calcium channel blocker, and thiazide diuretic) with estimated glomerular filtration rate (eGFR) of 40 mL/min/1.73 m2 or greater were randomly assigned to uRDN or sham with medications unchanged through 2 months. From 2 to 5 months, if monthly home BP was 135/85 mm Hg or higher, standardized stepped-care antihypertensive treatment starting with aldosterone antagonists was initiated under blinding to treatment assignment. Interventions: uRDN vs sham procedure in conjunction with added medications to target BP control. Main Outcomes and Measures: Six-month change in medications, change in daytime ambulatory systolic BP, change in home systolic BP adjusted for baseline BP and medications, and safety. Results: A total of 65 of 69 participants in the uRDN group and 64 of 67 participants in the sham group (mean [SD] age, 52.4 [8.3] years; 104 male [80.6%]) with a mean (SD) eGFR of 81.5 (22.8) mL/min/1.73 m2had 6-month daytime ambulatory BP m

Published
2022

21. Effects of Renal Denervation vs Sham in Resistant Hypertension After Medication Escalation: Prespecified Analysis at 6 Months of the RADIANCE-HTN TRIO Randomized Clinical Trial

Author

Circulatory Health, Team Medisch, Azizi, Michel, Mahfoud, Felix, Weber, Michael A, Sharp, Andrew S P, Schmieder, Roland E, Lurz, Philipp, Lobo, Melvin D, Fisher, Naomi D L, Daemen, Joost, Bloch, Michael J, Basile, Jan, Sanghvi, Kintur, Saxena, Manish, Gosse, Philippe, Jenkins, J Stephen, Levy, Terry, Persu, Alexandre, Kably, Benjamin, Claude, Lisa, Reeve-Stoffer, Helen, McClure, Candace, Kirtane, Ajay J, RADIANCE-HTN Investigators, Circulatory Health, Team Medisch, Azizi, Michel, Mahfoud, Felix, Weber, Michael A, Sharp, Andrew S P, Schmieder, Roland E, Lurz, Philipp, Lobo, Melvin D, Fisher, Naomi D L, Daemen, Joost, Bloch, Michael J, Basile, Jan, Sanghvi, Kintur, Saxena, Manish, Gosse, Philippe, Jenkins, J Stephen, Levy, Terry, Persu, Alexandre, Kably, Benjamin, Claude, Lisa, Reeve-Stoffer, Helen, McClure, Candace, Kirtane, Ajay J, and RADIANCE-HTN Investigators

Published
2022

22. Predictors of blood pressure response to ultrasound renal denervation in the RADIANCE-HTN SOLO study

Author

MS Nefrologie, Circulatory Health, Saxena, Manish, Schmieder, Roland E., Kirtane, Ajay J., Mahfoud, Felix, Daemen, Joost, Basile, Jan, Lurz, Philipp, Gosse, Philippe, Sanghvi, Kintur, Fisher, Naomi D.L., Rump, Lars C., Pathak, Atul, Blankestijn, Peter J., Mathur, Anthony, Wang, Yale, Weber, Michael A., Sharp, Andrew S.P., Bloch, Michael J., Barman, Neil C., Claude, Lisa, Song, Yang, Azizi, Michel, Lobo, Melvin D., MS Nefrologie, Circulatory Health, Saxena, Manish, Schmieder, Roland E., Kirtane, Ajay J., Mahfoud, Felix, Daemen, Joost, Basile, Jan, Lurz, Philipp, Gosse, Philippe, Sanghvi, Kintur, Fisher, Naomi D.L., Rump, Lars C., Pathak, Atul, Blankestijn, Peter J., Mathur, Anthony, Wang, Yale, Weber, Michael A., Sharp, Andrew S.P., Bloch, Michael J., Barman, Neil C., Claude, Lisa, Song, Yang, Azizi, Michel, and Lobo, Melvin D.

Published
2022

23. Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus.

Author

Mollov, Dimitre, Filloux, Denis, Grinstead, Samuel, Bolus, Stephen, Claude, Lisa, Fernandez, Emanuel, Julian, Charlotte, Candresse, Thierry, Lockhart, Ben, Roumagnac, Philippe, Rott, Philippe, and Daugrois, Jean Heinrich

Subjects
SUGARCANE, WHOLE genome sequencing, MOSAIC viruses, SUGARCANE growing, NUCLEOTIDE sequencing, GENE targeting
Abstract

The complete genome sequence of a previously uncharacterized sugarcane‐infecting virus of the genus Ampelovirus was obtained by high‐throughput sequencing (HTS) and rapid amplification of cDNA ends. This genome consists of 13,114 nucleotides (nt) and harbours seven open reading frames. Phylogenetic and pairwise identity analyses with the complete or near‐complete genome sequences of 16 isolates revealed that they all belong to a new Ampelovirus species, family Closteroviridae. These 16 isolates were distributed in three phylogenetic groups and potential recombinant events were identified for only one of these isolates. An immunocapture‐reverse transcription‐PCR (IC‐RT‐PCR) assay was developed with antibodies previously produced against a clostero‐like virus that had not yet been recognized by the International Committee on Taxonomy of Viruses but for which the name sugarcane mild mosaic virus (SCMMV) had been proposed. The new ampelovirus was detected by IC‐RT‐PCR, and Sanger‐sequenced amplicons confirmed the identity of the virus based on HTS data. SCMMV was detected in 65 of 350 samples of quarantined sugarcane germplasm using RT‐PCR targeting the hsp70 gene. These samples originated from Africa, the Caribbean, North America, South America and South‐east Asia, thus revealing that SCMMV is present in many sugarcane‐growing regions of the world. The complete or near‐complete sequences reported here represent the first genomic sequences for SCMMV. Furthermore, the newly developed RT‐PCR diagnostic assay will facilitate the understanding of SCMMV epidemiology and will help improve management practices. [ABSTRACT FROM AUTHOR]

Published
2023
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24. Plasma renin and aldosterone concentrations related to endovascular ultrasound renal denervation in the RADIANCE-HTN SOLO trial

Author

Fisher, Naomi D.L., primary, Kirtane, Ajay J., additional, Daemen, Joost, additional, Rader, Florian, additional, Lobo, Melvin D., additional, Saxena, Manish, additional, Abraham, Josephine, additional, Schmieder, Roland E., additional, Sharp, Andrew S.P., additional, Gosse, Philippe, additional, Claude, Lisa, additional, Song, Yang, additional, and Azizi, Michel, additional

Published
2021
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25. Comparison of the virome of quarantined sugarcane varieties and the virome of grasses growing near the quarantine station

Author

Daugrois, Jean, Filloux, Denis, Julian, Charlotte, Claude, Lisa, Ferdinand, Romain, Fernandez, Emmanuel, Fontes, Hugo, Rott, Philippe, Roumagnac, Philippe, Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut de recherche de la Tour du Valat, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Agropolis Foundation (E-Space flagship program, grant number 1504-004), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU)

Subjects
H01 - Protection des végétaux - Considérations générales, quarantaine, Poaceae, Microbiology, Article, Maladie des plantes, sugarcane, Phylogeny, Transmission des maladies, Plant Diseases, H20 - Maladies des plantes, Virome, Virion, Virus des végétaux, QR1-502, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Saccharum, plant quarantine, Quarantine, Mesure phytosanitaire, Metagenome, Metagenomics, viral metagenomics
Abstract

International audience; Visacane is a sugarcane quarantine station located in the South of France, far away from sugarcane growing areas. Visacane imports up to 100 sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new sugarcane infections and potentially cause new sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented.

Published
2021

26. Comparison of the virome of quarantined sugarcane varieties and the virome of grasses growing near the quarantine station

Author

Daugrois, Jean-Heinrich, Filloux, Denis, Julian, Charlotte, Claude, Lisa, Ferdinand, Romain, Fernandez, Emmanuel, Fontes, Hugo, Rott, Philippe, Roumagnac, Philippe, Daugrois, Jean-Heinrich, Filloux, Denis, Julian, Charlotte, Claude, Lisa, Ferdinand, Romain, Fernandez, Emmanuel, Fontes, Hugo, Rott, Philippe, and Roumagnac, Philippe

Abstract

Visacane is a sugarcane quarantine station located in the South of France, far away from sugarcane growing areas. Visacane imports up to 100 sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new sugarcane infections and potentially cause new sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented.

Published
2021

27. Predictors of blood pressure response to ultrasound renal denervation in the RADIANCE-HTN SOLO study

Author

Saxena, Manish, primary, Schmieder, Roland E., additional, Kirtane, Ajay J., additional, Mahfoud, Felix, additional, Daemen, Joost, additional, Basile, Jan, additional, Lurz, Philipp, additional, Gosse, Philippe, additional, Sanghvi, Kintur, additional, Fisher, Naomi D. L., additional, Rump, Lars C., additional, Pathak, Atul, additional, Blankestijn, Peter J., additional, Mathur, Anthony, additional, Wang, Yale, additional, Weber, Michael A., additional, Sharp, Andrew S. P., additional, Bloch, Michael J., additional, Barman, Neil C., additional, Claude, Lisa, additional, Song, Yang, additional, Azizi, Michel, additional, and Lobo, Melvin D., additional

Published
2021
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28. An atypical class of non-coding small RNAs produced in rice leaves upon bacterial infection

Author

Reshetnyak, Ganna, primary, Jacobs, Jonathan M., additional, Auguy, Florence, additional, Sciallano, Coline, additional, Claude, Lisa, additional, Medina, Clemence, additional, Perez-Quintero, Alvaro L., additional, Comte, Aurore, additional, Thomas, Emilie, additional, Bogdanove, Adam, additional, Koebnik, Ralf, additional, Szurek, Boris, additional, Dievart, Anne, additional, Brugidou, Christophe, additional, Lacombe, Severine, additional, and Cunnac, Sebastien, additional

Published
2021
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29. 12-Month Results From the Unblinded Phase of the RADIANCE-HTN SOLO Trial of Ultrasound Renal Denervation

Author

Azizi, Michel, primary, Daemen, Joost, additional, Lobo, Melvin D., additional, Mahfoud, Felix, additional, Sharp, Andrew S.P., additional, Schmieder, Roland E., additional, Wang, Yale, additional, Saxena, Manish, additional, Lurz, Philipp, additional, Sayer, Jeremy, additional, Bloch, Michael J., additional, Basile, Jan, additional, Weber, Michael A., additional, Rump, Lars C., additional, Levy, Terry, additional, Sapoval, Marc, additional, Sanghvi, Kintur, additional, Rader, Florian, additional, Fisher, Naomi D.L., additional, Gosse, Philippe, additional, Abraham, Josephine, additional, Claude, Lisa, additional, Barman, Neil C., additional, McClure, Candace K., additional, Liu, Yuyin, additional, Kirtane, Ajay J., additional, Chertow, Glenn, additional, Kahan, Thomas, additional, Dauerman, Harold, additional, and Ullery, Steven, additional

Published
2020
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31. Reverse transcriptase template switching: a method for without a priori amplification of full-length genome of positive-sense single-strand polyadenylated RNA plant viruses. [P.51]

Author

Claude, Lisa, Fernandez, Emmanuel, Roumagnac, Philippe, and Filloux, Denis

Abstract

In the absence of sequence information, obtaining the full-length sequence of RNA plant virus genomes is a real challenge. These viruses have linear genomes, sometimes relatively long (up to ~ 20 kb), and variable sequence ends which makes any PCR amplification impossible without a priori. While 3 'ends of polyadenylated viruses can be obtained by exploiting the presence of the poly-A tail, obtaining the 3' ends of non-polyadenylated viruses and 5 'ends requires artificial polyadenylation using, for example, Poly (U) Polymerase (3'RACE) and Terminal Transferase (5'RACE), respectively. We present here a method that can potentially amplify the full-length sequence of RNA plant virus genomes. We first focus towards obtaining the complete genome of positive-sense single-strand polyadenylated RNA plant viruses. The method called reverse transcriptase template switching, simultaneously uses the reverse transcriptase and template switching activities of the Moloney murine leukemia virus Reverse Transcriptase to synthesize in one step cDNAs flanked at each end by arbitrary sequences called anchors. These anchors then allow long-range PCR amplification of the cDNAs. The product of this amplification has been particularly adapted to be sequenced by the Nanopore technology which allows the sequencing of long fragments (100 bp to >20 kb). We here show that the nearly full-length sequences of both yam mild mosaic virus (Potyvirus genus) and yam chlorotic necrosis virus (Macluravirus genus), members of the large and socio-economically relevant Potyviridae family, could be obtained using the reverse transcriptase template switching approach followed by Nanopore sequencing. Studies aiming at obtaining full-length sequence of non-polyadenylated RNA plant virus genomes are underway.

Published
2019

32. 36-month durability of ultrasound renal denervation for hypertension resistant to combination therapy in RADIANCE-HTN TRIO.

Author

Bloch MJ, Kirtane AJ, Azizi M, Mahfoud F, Basile J, Daemen J, Saxena M, Thackeray L, McGuire M, Claude L, and Schmieder RE

Abstract

Endovascular ultrasound renal denervation (uRDN) reduced blood pressure (BP) compared to sham at 2 months in patients with resistant hypertension in the multicenter, blinded, randomized, sham-controlled RADIANCE-HTN TRIO trial. This analysis evaluates longer-term outcomes of patients randomized to uRDN. Patients with resistant hypertension to a 3-drug combination pill were randomized to uRDN (n = 69) or sham (n = 67). From 2-5 months, patients followed a standardized anti-hypertensive medication (AHM) titration protocol. At 6 months, patients were unblinded and received AHM per standard of care. In the uRDN group, 71% (49/69) completed 36-month follow-up. Screening office BP was 159/103 on 3.9 AHM. Baseline office BP on the single-pill combination was 153/99 mmHg. At 36 months, office BP changed by -14.5 ± 26.1/-9.0 ± 14.8 mmHg from screening (p < 0.001 for both) and -8.0 ± 24.5/-5.0 ± 14.6 mmHg from baseline (p = 0.007; p = 0.022) on 3.7 AHM. The efficacy of uRDN was durable to 36 months in patients with resistant hypertension with no safety concerns., (© 2024. The Author(s).)

Published
2024
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33. Endovascular Ultrasound Renal Denervation to Treat Hypertension: The RADIANCE II Randomized Clinical Trial.

Author

Azizi M, Saxena M, Wang Y, Jenkins JS, Devireddy C, Rader F, Fisher NDL, Schmieder RE, Mahfoud F, Lindsey J, Sanghvi K, Todoran TM, Pacella J, Flack J, Daemen J, Sharp ASP, Lurz P, Bloch MJ, Weber MA, Lobo MD, Basile J, Claude L, Reeve-Stoffer H, McClure CK, and Kirtane AJ

Subjects
Female, Humans, Male, Middle Aged, Antihypertensive Agents therapeutic use, Endovascular Procedures, Kidney diagnostic imaging, Kidney innervation, Vascular Surgical Procedures, Single-Blind Method, Denervation methods, Hypertension surgery, Ultrasonography, Interventional methods
Abstract

Importance: Two initial sham-controlled trials demonstrated that ultrasound renal denervation decreases blood pressure (BP) in patients with mild to moderate hypertension and hypertension that is resistant to treatment., Objective: To study the efficacy and safety of ultrasound renal denervation without the confounding influence of antihypertensive medications in patients with hypertension., Design, Setting, and Participants: Sham-controlled, randomized clinical trial with patients and outcome assessors blinded to treatment assignment that was conducted between January 14, 2019, and March 25, 2022, at 37 centers in the US and 24 centers in Europe, with randomization stratified by center. Patients aged 18 years to 75 years with hypertension (seated office systolic BP [SBP] ≥140 mm Hg and diastolic BP [DBP] ≥90 mm Hg despite taking up to 2 antihypertensive medications) were eligible if they had an ambulatory SBP/DBP of 135/85 mm Hg or greater and an SBP/DBP less than 170/105 mm Hg after a 4-week washout of their medications. Patients with an estimated glomerular filtration rate of 40 mL/min/1.73 m2 or greater and with suitable renal artery anatomy were randomized 2:1 to undergo ultrasound renal denervation or a sham procedure. Patients were to abstain from antihypertensive medications until the 2-month follow-up unless prespecified BP criteria were exceeded and were associated with clinical symptoms., Interventions: Ultrasound renal denervation vs a sham procedure., Main Outcomes and Measures: The primary efficacy outcome was the mean change in daytime ambulatory SBP at 2 months. The primary safety composite outcome of major adverse events included death, kidney failure, and major embolic, vascular, cardiovascular, cerebrovascular, and hypertensive events at 30 days and renal artery stenosis greater than 70% detected at 6 months. The secondary outcomes included mean change in 24-hour ambulatory SBP, home SBP, office SBP, and all DBP parameters at 2 months., Results: Among 1038 eligible patients, 150 were randomized to ultrasound renal denervation and 74 to a sham procedure (mean age, 55 years [SD, 9.3 years]; 28.6% female; and 16.1% self-identified as Black or African American). The reduction in daytime ambulatory SBP was greater with ultrasound renal denervation (mean, -7.9 mm Hg [SD, 11.6 mm Hg]) vs the sham procedure (mean, -1.8 mm Hg [SD, 9.5 mm Hg]) (baseline-adjusted between-group difference, -6.3 mm Hg [95% CI, -9.3 to -3.2 mm Hg], P < .001), with a consistent effect of ultrasound renal denervation throughout the 24-hour circadian cycle. Among 7 secondary BP outcomes, 6 were significantly improved with ultrasound renal denervation vs the sham procedure. No major adverse events were reported in either group., Conclusions and Relevance: In patients with hypertension, ultrasound renal denervation reduced daytime ambulatory SBP at 2 months in the absence of antihypertensive medications vs a sham procedure without postprocedural major adverse events., Trial Registration: ClinicalTrials.gov Identifier: NCT03614260.

Published
2023
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34. Effects of Renal Denervation vs Sham in Resistant Hypertension After Medication Escalation: Prespecified Analysis at 6 Months of the RADIANCE-HTN TRIO Randomized Clinical Trial.

Author

Azizi M, Mahfoud F, Weber MA, Sharp ASP, Schmieder RE, Lurz P, Lobo MD, Fisher NDL, Daemen J, Bloch MJ, Basile J, Sanghvi K, Saxena M, Gosse P, Jenkins JS, Levy T, Persu A, Kably B, Claude L, Reeve-Stoffer H, McClure C, and Kirtane AJ

Subjects
Humans, Male, Middle Aged, Mineralocorticoid Receptor Antagonists therapeutic use, Treatment Outcome, Denervation methods, Antihypertensive Agents therapeutic use, Hypertension drug therapy, Hypertension surgery
Abstract

Importance: Although early trials of endovascular renal denervation (RDN) for patients with resistant hypertension (RHTN) reported inconsistent results, ultrasound RDN (uRDN) was found to decrease blood pressure (BP) vs sham at 2 months in patients with RHTN taking stable background medications in the Study of the ReCor Medical Paradise System in Clinical Hypertension (RADIANCE-HTN TRIO) trial., Objectives: To report the prespecified analysis of the persistence of the BP effects and safety of uRDN vs sham at 6 months in conjunction with escalating antihypertensive medications., Design, Setting, and Participants: This randomized, sham-controlled, clinical trial with outcome assessors and patients blinded to treatment assignment, enrolled patients from March 11, 2016, to March 13, 2020. This was an international, multicenter study conducted in the US and Europe. Participants with daytime ambulatory BP of 135/85 mm Hg or higher after 4 weeks of single-pill triple-combination treatment (angiotensin-receptor blocker, calcium channel blocker, and thiazide diuretic) with estimated glomerular filtration rate (eGFR) of 40 mL/min/1.73 m2 or greater were randomly assigned to uRDN or sham with medications unchanged through 2 months. From 2 to 5 months, if monthly home BP was 135/85 mm Hg or higher, standardized stepped-care antihypertensive treatment starting with aldosterone antagonists was initiated under blinding to treatment assignment., Interventions: uRDN vs sham procedure in conjunction with added medications to target BP control., Main Outcomes and Measures: Six-month change in medications, change in daytime ambulatory systolic BP, change in home systolic BP adjusted for baseline BP and medications, and safety., Results: A total of 65 of 69 participants in the uRDN group and 64 of 67 participants in the sham group (mean [SD] age, 52.4 [8.3] years; 104 male [80.6%]) with a mean (SD) eGFR of 81.5 (22.8) mL/min/1.73 m2 had 6-month daytime ambulatory BP measurements. Fewer medications were added in the uRDN group (mean [SD], 0.7 [1.0] medications) vs sham (mean [SD], 1.1 [1.1] medications; P = .045) and fewer patients in the uRDN group received aldosterone antagonists at 6 months (26 of 65 [40.0%] vs 39 of 64 [60.9%]; P = .02). Despite less intensive standardized stepped-care antihypertensive treatment, mean (SD) daytime ambulatory BP at 6 months was 138.3 (15.1) mm Hg with uRDN vs 139.0 (14.3) mm Hg with sham (additional decreases of -2.4 [16.6] vs -7.0 [16.7] mm Hg from month 2, respectively), whereas home SBP was lowered to a greater extent with uRDN by 4.3 mm Hg (95% CI, 0.5-8.1 mm Hg; P = .03) in a mixed model adjusting for baseline and number of medications. Adverse events were infrequent and similar between groups., Conclusions and Relevance: In this study, in patients with RHTN initially randomly assigned to uRDN or a sham procedure and who had persistent elevation of BP at 2 months after the procedure, standardized stepped-care antihypertensive treatment escalation resulted in similar BP reduction in both groups at 6 months, with fewer additional medications required in the uRDN group., Trial Registration: ClinicalTrials.gov Identifier: NCT02649426.

Published
2022
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35. Virion-Associated Nucleic Acid-Based Metagenomics: A Decade of Advances in Molecular Characterization of Plant Viruses.

Author

Moubset O, François S, Maclot F, Palanga E, Julian C, Claude L, Fernandez E, Rott P, Daugrois JH, Antoine-Lorquin A, Bernardo P, Blouin AG, Temple C, Kraberger S, Fontenele RS, Harkins GW, Ma Y, Marais A, Candresse T, Chéhida SB, Lefeuvre P, Lett JM, Varsani A, Massart S, Ogliastro M, Martin DP, Filloux D, and Roumagnac P

Subjects
Metagenomics methods, Ecosystem, Plant Diseases, Virion genetics, Plants, Nucleic Acids, Plant Viruses genetics
Abstract

Over the last decade, viral metagenomic studies have resulted in the discovery of thousands of previously unknown viruses. These studies are likely to play a pivotal role in obtaining an accurate and robust understanding of how viruses affect the stability and productivity of ecosystems. Among the metagenomics-based approaches that have been developed since the beginning of the 21st century, shotgun metagenomics applied specifically to virion-associated nucleic acids (VANA) has been used to disentangle the diversity of the viral world. We summarize herein the results of 24 VANA-based studies, focusing on plant and insect samples conducted over the last decade (2010 to 2020). Collectively, viruses from 85 different families were reliably detected in these studies, including capsidless RNA viruses that replicate in fungi, oomycetes, and plants. Finally, strengths and weaknesses of the VANA approach are summarized and perspectives of applications in detection, epidemiological surveillance, environmental monitoring, and ecology of plant viruses are provided. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Published
2022
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36. Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station.

Author

Daugrois JH, Filloux D, Julian C, Claude L, Ferdinand R, Fernandez E, Fontes H, Rott PC, and Roumagnac P

Subjects
Phylogeny, Plant Diseases virology, Quarantine, Virion, Metagenome, Metagenomics methods, Poaceae virology, Saccharum virology, Virome
Abstract

Visacane is a sugarcane quarantine station located in the South of France, far away from sugarcane growing areas. Visacane imports up to 100 sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new sugarcane infections and potentially cause new sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented.

Published
2021
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