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Transforming growth factor-beta (TGF-beta) is a potent inhibitor of growth factor-stimulated hematopoiesis in normal and leukemic conditions. Using the factor-dependent myelogenous leukemia cell lines GF-D8 and Mo7, we show that TGF-beta interferes with stem cell factor (SCF)-induced proliferation by downmodulating c-jun gene expression. The ability of SCF to induce accumulation of c-jun transcripts was abolished when TGF-beta was present in culture. Transcriptional nuclear run-on assays indicated that TGF-beta relieved the capacity of SCF to enhance the transcriptional rate of the c-jun gene. Deletion analysis of the c-jun promoter furthermore showed that SCF was activating the c- jun promoter via the NF-jun transcription factor. Gel mobility shift assays showed that SCF increased the binding activity of NF-jun to its recognition site within 5 to 15 minutes. Binding activity peaked at 1 hour after exposure to SCF and declined to starting levels within 4 hours. The ability of SCF to enhance NF-jun binding activity was also dose-dependent in the range of 5 to 100 ng/mL. Exposure of GF-D8 and Mo7 cells to TGF-beta before the addition of SCF antagonized SCF- induced NF-jun binding. Moreover, whereas SCF was capable of functionally activating a heterologous promoter containing the NF-jun binding site, pretreatment of GF-D8 cells with TGF-beta abolished transcriptional activation of this heterologous promoter. These findings indicate that SCF-mediated activation of c-jun via NF-jun is crucial for the SCF-inducible proliferative response and is inhibited by TGF-beta. In additional experiments, the antisense technique was used. Treatment of GF-D8 and Mo7 cells with an antisense oligodeoxyribonucleotide directed against the translation initiation site of c-jun abolished the capacity of SCF to induce a proliferative response, whereas sense and nonsense oligomers had no effect. Taken together, our data indicate that the counteracting modulation of the binding activity of NF-jun by SCF and TGF-beta regulates the expression of the c-jun gene and thereby the proliferative state of the GF-D8 and Mo7 target.

Transforming growth factor (TGF)-alpha is a pleiotropic polypeptide which mediates a variety of tissue-specific cellular responses such as induction of proliferation, cell migration, vascularization, and formation of extracellular matrix. TGF-alpha is produced by certain tumor cells and embryogenic tissues, as well as by normal cells of different origin. Within the granulocytic lineage, TGF-alpha production has been shown in promyelocytic leukemia cells induced to differentiate, as well as in blood eosinophils of patients with the idiopathic hypereosinophilic syndrome. The present study was carried out in order to examine expression of the TGF-alpha gene in polymorphonuclear (PMN) and mononuclear (MN) blood cells of normal healthy donors. While MN and neutrophilic PMN failed to synthesize TGF-alpha transcripts and protein, eosinophils constitutively exhibited TGF-alpha transcripts accompanied by the release of immunoreactive TGF-alpha protein. Exposure of PMN and MN cells to the leukocyte-activating cytokines interleukin (IL)-3, IL-5, and granulocyte-macrophage colony-stimulating factor resulted in a several-fold increase of TGF-alpha mRNA expression and protein release by eosinophils, but not by neutrophils and MN cells. PMN and MN were insensitive to induction of TGF-alpha release by IL-8 and granulocyte colony-stimulating factor. These results point to a functional role of eosinophils in disorders characterized by unbalanced TGF-alpha production such as disease states associated with abnormal matrix formation and neovascularization which may be explained by the present demonstration of TGF-alpha production in these cells.

Die normale Zelle unterliegt in ihrem biologischen Verhalten der Kontrolle extrazellularer Signale. Die Einsicht in die Prinzipien der Signalerkennung und Verarbeitung, in die Regeln der interzellularen Kommunikation und des intrazellularen Zusammenspiels von Proteinen mit Proteinen und DNS wird zusehends erweitert und vertieft. In Einzelfallen ist es moglich, die Schritte der Signaltransduktion. von der Ligandbindung uber die Rezeptoraktivierung und Aufschlusselung der an der Signalubertragung teilhabendenen Molekule bis hin zur Aktivierung distinkter genetischer Programme im Kern zu verfolgen. Errungen wurde dieser Kenntnisgewinn durch detaillierte biochemische und molekularbiologische Untersuchungen intrazellularer Vorgange und nicht zuletzt mit der durch Kristallographie und NMR-Technologie ermoglichten Aufklarung der dreidimensionalen Struktur von Molekulen und ihren Interaktionen. Entscheidende Hinweise fur die Aufdeckung von Protein-Funktionen und Protein-Interaktionen kamen zudem aus der molekularbiologischen und biochemischen Charakterisierung genetischer Veranderungen, die Tumorentstehung und Tumorprogression begleiten. Dies hat es erlaubt, nicht nur eine grosere Anzahl von (Krebs)genen zu identifizieren, ihre genetische Information zu entschlusseln und die biologische Funktion ihrer Proteine zu charakterisieren, sondern hat auch unser Verstandnis physiologischer Regulationsmechanismen grundlegend erweitert. Die Kenntnis der molekularen Zusammenhange von Krebsentstehung und Krebsprogression ist eine solide Grundlage fur die Entwicklung neuer therapeutischer Interventionsmoglichkeiten, die neben gentherapeutischen Ansatzen auch die Entwicklung neuartiger Wirksubstanzen umfassen.