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We tested whether selection of ryegrass for reduced root hair length and density would reduce root competition on white clover for phosphate (P). A Rhizosheath Selection Tool was used to select two ryegrass populations with contrasting root hair traits. These were grown at five soil P levels in a glasshouse experiment and rhizosheath trait selection had no effect on growth or shoot P concentrations. The ryegrass populations were also grown with white clover in soils with Olsen P12 or 19 mg L−1. In the Olsen P12 soil, clover grown with the smaller rhizosheath ryegrass had significantly heavier shoots and contained more P than clover grown with the larger rhizosheath ryegrass. In the Olsen P19 soil differences in ryegrass rhizosheath had no effect on clover growth. The results showed that under the experimental conditions, grass-clover competition for soil P could be modified by the rhizosheath traits of ryegrass. [ABSTRACT FROM AUTHOR]

Sugar maples (Acer saccharum Marshall) develop elevated stem pressures in springtime through the compression and expansion of gas bubbles present within xylem fibres. The stability of this gas within the fibres is hypothesised to be due to the elevated sugar concentration of maple sap and the presence of an osmotic barrier between fibres and vessels. Without this osmotic barrier gas bubbles are predicted to dissolve rapidly. In this work we investigated the existence of this osmotic barrier. We quantified the fraction of the xylem occupied by gas-filled fibres using synchrotron based microCT. After imaging fresh stem segments we perfused them with either a 2% sucrose solution or water, imaging again following perfusion. In this way we directly observed how total gas present in the fibres changed when an osmotic pressure difference should be present, with the 2% sucrose solution, and when it is absent, with the water. Following a first round of perfusion we perfused stem segments with the other perfusate, repeating this multiple times to observe how switching perfusates affected gas-filled fibres. We found that perfusing stem segments with water resulted in a significant reduction in the xylem fibre gas, but perfusing stem segments with a sucrose solution did not significantly reduce the gas in the fibres. These results support the hypothesis that an osmotic barrier exists between fibres and vessels., (© The Author(s) 2024. Published by Oxford University Press.)