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Culex pipiens (Linnaeus), one of the most abundant mosquito species in Europe, plays a crucial role in the endemic transmission of West Nile virus and caused the large outbreak with >1600 human cases in 2018. Although evidence of resistance to pyrethroids has been reported for Cx. pipiens populations from Spain and Greece, resistance monitoring has been largely neglected in Italy. Herein, we investigate susceptibility of Italian Cx. pipiens populations to the pyrethroids permethrin and deltamethrin. Results from WHO‐tube‐bioassays revealed mortalities ranging from 14–54%, indicating high levels of resistance, in four out of 10 populations exposed to permethrin (0.75%) and of 63% in one of three populations exposed to deltamethrin (0.05%). Reduced susceptibility (mortality<98%) was detected in almost all other populations. A clear association is shown between the resistant phenotype and the presence of kdr‐alleles in position 1014 of the VSSC, strongly suggesting its role in reducing susceptibility. The study provides the first evidence of pyrethroid‐resistance in Italian Cx. pipiens populations and reports levels of resistance paralleled in the European region only in Turkey. This highlights the urgent need to implement insecticide‐resistance management plans to restore the efficacy of the nowadays only chemical weapon available to control arbovirus transmission in Europe. [ABSTRACT FROM AUTHOR]

Background: WHO guidelines state that adulticide interventions are recommended only in case of disease outbreak. However, peridomestic sprayings are carried out routinely to reduce Aedes albopictus (Skuse) nuisance, at least in Italy. Failing to keep low adult abundance over time triggers the need for further applications. The aim of the study was to investigate the effectiveness of a common control strategy routinely performed by citizens in highly infested urban sites inside the metropolitan city of Rome using a freely purchasable pyrethroid and a hand-held sprayer. Moreover, the effectiveness evaluated in three field experiments was compared with that achieved by blending the pyrethroid with a new carbon-based liquid additive., Results: An 86% post-treatment reduction in Ae. albopictus abundance was observed in gravid and host-seeking females, while the population recovery time was 10 days. Blending the insecticide with the additive lengthened mosquito recovery time to over 14 days., Conclusion: Peridomestic sprayings largely reduce mosquito populations immediately after treatment but fail to keep low mosquito abundance for a longer period, partially explaining the uncontrolled repetitions of treatments. An optimal control application could benefit from research in the field of additives to improve mosquito abatement and the performance of pyrethroids over time. © 2016 Society of Chemical Industry., (© 2016 Society of Chemical Industry.)