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Codonopsis foetens Hook.f. and C. thalictrifolia Wall., two high altitude plants occurring at an elevation of 3300–4800 m, are recorded from Arunachal Pradesh State in India for the first time. These species were collected from Nagula Wetland complex of Tawang District of the state. Detailed description, occurrence, distribution, flowering and fruiting season, and picture pale of the species are provided in this paper for their easy identification in the field.

Intestinal cancer is a disease with high morbidity and high mortality in China. Previous studies have shown that Codonopsis foetens can inhibit cellular autophagy and promote the apoptosis of intestine cancer cells. Based on metabolomics method coupled with liquid chromatography-mass spectrometry (LC-MS) technology, we aimed to analyze intestinal small molecule metabolites in the intestinal cancer model group and the Codonopsis foetens treated group. Principal component analysis (PCA) and Partial Least Squares (PLS-DA) were used to identify the pattern of the data. And the metabolic characteristics of the cancer model group were explored based on the metabolic differences between the groups. Multivariate statistical analysis revealed that metabolites presented with differences included: Acetamide, Phosphoric acid, Hydrogen sulfite, Pyruvic acid, Cytosine, 2-Hydroxypyridine, Phosphoric acid, Uracil, Gamma-Aminobutyric acid, Glycerol alpha-monochlorohydrin, Thiosulfic acid, L-Valine, Cysteamine, Taurine, Creatine, Homocysteine, Hypoxanthine, Se-Methylselenocysteine, 5-Hydroxymethyluracil, Oxoglutaric acid, LysoPC(20:0), LysoPC(22:4(7Z,10Z,13Z,16Z)), LysoPC(18:2(9Z,12Z)), LysoPC(16:1(9Z)), LysoPE(0:0/16:0), LysoPE(0:0/18:2(9Z,12Z)), LysoPE(18:0/0:0), LysoPE(20:1(11Z)/0:0), etc. Combined with metabolic pathway analysis, pathways presented with differences included: Citrate cycle (TCA cycle), ABC transporters, 2-Oxocarboxylic acid metabolism, Taurine and hypotaurine metabolism, Butanoate metabolism), Phenylalanine, tyrosine and tryptophan biosynthesis, Biosynthesis of amino acids, Protein digestion and absorption, Aminoacyl-tRNA biosynthesis, C5-Branched dibasic acid metabolism, GABAergic synapse, Proximal tubule bicarbonate reclamation, Mineral absorption, Phenylalanine metabolism. The results showed that the proliferation of intestinal cancer cells caused cell metabolism disorders, manifesting as changes in metabolic pathways and resulting in changes in metabolites. [ABSTRACT FROM AUTHOR]

Abstract As a complicated micro-ecosystem, gut microbes are closely related to metabolic disease, immune disease and tumor (such as constipation. Long-term constipation would cause intestinal mucosal injury, enteritis, ileus, etc., thus inducing intestine cancer). In this research, intestine cancer model group and Codonopsis foetens treatment group were successfully constructed, and the variation of intestinal microbes were analyzed by 16S rRNA sequence. Results showed that there were changes in bacteria abundance of Firmicutes, Bacteroidetes, Proteobacteria, Deferribacteres, Tenericutes, and Actinobacteria, etc. Codonopsis foetens could directly or indirectly affect the growth and metabolism of Deferribacteres by altering the nutritional ingredient and pH value of intestine "medium", thus affecting the occurrence and development of intestinal microbes. [ABSTRACT FROM AUTHOR]