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Advances in cryobiology techniques commonly target either the cooling or the warming cycle, while little thought has been given to ≪repair≫ protocols applicable during cold storage. In particular, crystallization is the dominant threat to cryopreserved samples but proceeds from small nuclei that are innocuous if further growth is forestalled. To this end, we propose a laser editing technique that locally heats individual crystals above their melting point by a focused nanosecond pulse, followed by amorphization during rapid resolidification. As a reference, we first apply the approach to ice crystals in cryoprotected solution and use Raman confocal mapping to study the deactivation of crystalline order. Then, we examine dimethyl sulfoxide trihydrate crystals that can germinate at low temperatures in maximally freeze concentrated regions, as commonly produced by equilibrium cooling protocols. We show how to uniquely identify this phase from Raman spectra and evidence retarded growth of laser-edited crystals during warming. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study investigated the antioxidant and antimicrobial properties, as well as the volatile compounds, of Lactobacillus bulgaricus (L. bulgaricus) postbiotics (at concentration of 150 and 300 mg/L) and their combination with chitosan coatings (0.5% and 1%) on sausage quality (with 100 ppm nitrite) during 40 days of cold storage. The results were compared to a control group, as well as to sausages containing commercial formulation (120 ppm) and reduced (100 ppm) levels of nitrite. To further assess the antimicrobial effects, it also inoculated E. coli and Staphylococcus aureus into the sausages in order to examine how the postbiotics and chitosan coatings impacted the growth of these foodborne pathogens during the 40-day cold storage period. The reults indicated that those containing 300 mg/L postbiotic and 1% chitosan generally met the desired condition for pH, moisture, fat, and total volatile base-nitrogen. These samples also showed the strongest inhibition of mesophilic and psychrophilic bacteria, mold and yeast. Notably, no E. coli or S. aureus were detected in any of the samples, indicating that the postbiotic and chitosan combination effectively inhibited the growth of these pathogens in sausages. The findings suggest that using chitosan coatings and L. bulgaricus postbiotic can enhance the quality of sausages, ultimately lowering the risk of contamination by harmful bacteria and improving overall food safety. [ABSTRACT FROM AUTHOR]

Engineering design practices in STEM education focus on reflecting the discipline-specific work of engineers in a manner that is suitable for the student level and course learning objectives. Using an engineering design context also facilitates student identification of global problems and feasible solutions to address such problems. In this article, within the framework of STEM approach, an engineering design activity that can be applied in middle school mathematics course is presented. Additionally, examples of students' experiences of the engineering design-based mathematics activity are given. The "Cold Storage Design Activity" was executed in a classroom setting, and the implementation process underwent examination and evaluation. 12 eighth-grade students from a middle school in Turkey took part in the implementation. The middle school were able to successfully perform an engineering design process and effectively overcome challenges through teamwork. The results of the activity show that middle school students are excited and eager to participate in a design activity. [ABSTRACT FROM AUTHOR]

In this study, an optimization strategy of mechanical ventilation was carried out to reduce frost heaving and heating power in a large cold storage operation. Then, a response surface method is used to determine influential factors and mechanisms of mechanical ventilation by using a standard pre-assembled cold storage system as a test case. Results show that the main factors affecting the soil surface temperature critical to frost-heaving prevention include the soil temperature, airflow speed, pipe diameter, and pipe spacing. For a case study, the spacing of the ventilation pipes has the most significant influence on the surface temperature of the soil layer. In contrast, the soil temperature at a depth of 3.2 m has a minor impact on the surface temperature. Especially for the design criteria that the soil surface temperature cannot be less than 2 °C, the diameter of the ventilation pipe should be greater than 0.28 m, the spacing of the ventilation pipe should be less than 1.01 m and the airflow speed should be greater than 2.09 m·s−1. [ABSTRACT FROM AUTHOR]

Background: Normothermic machine perfusion (NMP) aims to reduce ischemia–reperfusion injury in donor livers and its clinical manifestation, early allograft dysfunction (EAD) by maintaining perfusion and oxygenation. However, there is limited data on which NMP perfusate biomarkers might be associated with such EAD and the role of perfusate hemoglobin has not been assessed. Methods: We performed a pilot retrospective analysis of adult donor livers undergoing NMP between 2020 and 2022 at our center. NMP was commenced at the recipient hospital after initial static cold storage. All NMP circuits were primed in the same manner according to the manufacturer's instructions. Livers were stratified by initial perfusate hemoglobin below (≤5.2 mmol/L) or above (>5.2 mmol/L) the median. The association between hemoglobin levels and EAD or recipient peak transaminase levels was assessed. Results: Among 23 livers, eight were considered unsuitable for transplantation, leaving 15 livers for assessment. Higher initial hemoglobin was associated with a lower risk of EAD (0% vs. 55.6%, p = 0.04). Perfusate hemoglobin decreased after NMP initiation (p = 0.003) and negatively correlated with recipient peak transaminase levels (ALT: ρ = −0.72, p = 0.002; AST: ρ = −0.79, p < 0.001). Consistently, higher hemoglobin livers also demonstrated lower perfusate liver enzymes. Conclusions: Perfusate hemoglobin levels decreased during NMP, and lower perfusate hemoglobin levels were associated with a higher incidence of EAD and higher levels of liver injury markers. Maintaining higher hemoglobin levels during NMP may help reduce ischemia–reperfusion injury and prevent or attenuate EAD. Larger prospective studies are needed to validate the findings of this pilot study. [ABSTRACT FROM AUTHOR]

Background: The Organ Care System (OCS) (Transmedics, Andover, MA) reduces cold ischemic time of donor hearts by producing a normothermic beating state during ex vivo perfusion, enabling extended ex situ intervals, which potentially increases donor pool. We aimed to compare outcomes in utilization of OCS and conventional cold storage technique. Methods: Consecutive heart transplants following brain death at our institution between May 2022 and July 2023 were analyzed. Recipients were divided into those receiving hearts preserved with OCS [N = 15] and those with conventional cold storage (Control, N = 27), with OCS utilization when anticipated ischemic time was more than 4 h. Pre‐transplant characteristics and transplant outcomes were compared. Results: OCS utilization allowed a significant increase in distance traveled for heart retrieval (OCS, 624 ± 269 vs. Control, 153 ± 128 miles, p < 0.001), with longer mean total preservation times (6.2 ± 1.1 vs 2.6 ± 0.6 h, p < 0.001). All but one patient displayed a general decrease or plateau in lactate throughout perfusion time by OCS. Both groups experienced similar rates of severe primary graft dysfunction (OCS, 6.7% [N = 1] vs. Control, 11.1% [N = 3], p = 0.63), with 100% in‐hospital survival in the OCS group compared to 96.3% in the Control group (p = 0.34). Kaplan–Meier survival analysis showed that estimated one‐year survival were comparable (OCS, 93.3 ± 6.4% vs. Control, 88.9 ± 6.0%, p = 0.61). Conclusion: With a mean preservation time of around 6 h and distance covered of over 600 miles, our results using OCS indicate a potential to safely increase the quantity and viability of accessible organs, thus broadening the donor pool without negatively affecting outcomes. [ABSTRACT FROM AUTHOR]

Introduction: A growing interest in renal normothermic machine perfusion (NMP) has resulted in more clinically available perfusion devices. While all perfusion systems have the same aim, there are significant differences in their circuits, pumps, sensors, and software. Therefore, our objective was to assess the impact of different perfusion protocols and devices on kidney function and perfusion parameters during NMP. Methods: Porcine kidneys were subjected to 30 min of warm ischemia, 24 h of static cold storage, and subsequently perfused for 6 h using (1) the Kidney Assist (KA) machine with a pressure of 75 mm Hg, (2) the KA device incorporating several adjustments and a pressure of 85 mm Hg (modified KA), or (3) the Perlife (PL) perfusion device (n = 4). Consecutively, discarded human kidneys were perfused using the KA or modified KA (n = 3) protocol. Results: The PL group quickly reached the device's upper flow limit and consequently received a significantly lower pressure compared to the KA groups. The arterial pO2 was significantly lower in the PL group. Yet, hemoglobin concentration increased over time, and oxygen consumption was significantly higher compared to the KA groups. Fractional sodium excretion was significantly lower in the PL group. Tissue ATP levels, urine production, and creatinine clearance rates did not differ between groups. In human kidneys, the modified KA group showed significantly lower vascular resistance, higher oxygen delivery, and lower levels of lactate in the perfusate compared to the KA group. Conclusions: This study shows that perfusion characteristics and kidney function are significantly influenced by the perfusion protocol and the device and its settings during normothermic machine perfusion and therefore should be interpreted with caution. [ABSTRACT FROM AUTHOR]

• Muscadines show differences in bioactive compound levels across cultivars. • Purple cultivars display elevated levels of bioactive compounds compared to bronze ones. • Purple cultivars maintain higher levels of bioactive compounds than bronze cultivars. • Peel reveals superior levels of bioactive compounds and antioxidants compared to the flesh. • 'Supreme' demonstrates the highest storability, followed by 'Paulk' and 'Alachua'. The postharvest changes in bioactive compounds and antioxidant activity of the whole fruit, peel, and flesh, as well as color, weight and firmness changes, were investigated in six commercial muscadine cultivars (three purple: 'Alachua' (AL), 'Paulk' (PA), and 'Supreme' (SU); and three bronze: 'Granny Val' (GV), 'Hall' (HA), and 'Triumph' (TR) during cold storage at 4 °C for 42 days. Weight loss increased significantly (P < 0.05), with SU losing 5.79% and HA losing 8.23% by day 42. Firmness significantly (P < 0.05) decreased during cold storage, with SU displaying the higest (164.02 g mm−1) and HA the lowest (110.75 g mm−1) by the end of storage. Total soluble solids (TSS), titratable acidity (TA), and their ratio (TSS/TA) showed variations among cultivars and parts of the fruit, with purple cultivars consistently exhibiting higher titratable acidity (TA) compared to bronze cultivars, while bronze cultivars demonstrated higher TSS/TA ratios. TSS ranged from 13.25 to 16.8%, TA from 0.62 to 0.84%, and TSS/TA ratios varied from 20.53 to 41.23 across cultivars (in whole fruit). HA (15.32%) and TR (15.32%) exhibited the highest TSS content, while GV had the lowest TSS at 13.25%. In terms of TA, AL had the highest value at 0.86%, whereas HA (0.46%) and TR (0.51%) recorded the lowest TA. The TSS/TA ratio ranged from 18.31 in AL to 36.85 in HA, representing the lowest and highest ratios, respectively. Color parameters differed between purple and bronze cultivars, with purple cultivars displaying higher a * (red component), while bronze cultivars had higher L * and C * values (lighter and more pure color); the h ° values of the purple and bronze cultivars were also significantly (P < 0.05) different, but did not differ within each group. The purple cultivars had higher total anthocyanin concentration (TAC) (8.4–15.31 mg L−1), total phenolic content (TPC) (92.11–107.01 mg GAE L−1), and total antioxidant activity (TAA) (150.25–250.05 μmoles L−1) than the bronze cultivars, and the peel had higher levels of those bioactive compounds than the flesh. AL exhibited the highest TAC at 15.31 mg L−1, followed by SU (13.01 mg L−1) and PA (8.4 mg L−1). In terms of TPC, AL (107.01 mg GAE L−1) and SU (92.11 mg GAE L−1) had the highest levels, with PA (74.89 mg GAE L−1) following. Among the bronze cultivars, TR had the highest TPC (61.41 mg GAE L−1), while GV exhibited the lowest (35.09 mg GAE L−1). The TAA of the purple cultivars ranged from around 250 to 134 μmoles L−1 TE in whole fruit, 500 to 319 μmoles L−1 in the peel, and 17 to 10 μmoles L−1 in flesh, while the TAA of the bronze cultivars ranged from about 122 to 56 μmoles L−1 TE in whole fruit, 248 to 120 μmoles L−1 in the peel, and 16 to 10 μmoles L−1in the flesh. During cold storage, TAC, TPC, and TAA decreased in both the peel and the flesh, but more so in the peel. Two of the three purple cultivars (SU and AL) maintained more TAC, TPC, and TAA in the peel than the three bronze cultivars. Based on our results, the purple muscadine cultivar SU, followed by purple PA and AL, exhibited better storability than the bronze cultivars in terms of maintaining their quality factors closest to the initial values. Among the bronze cultivars, GV exhibited the best storability. Overall, the order of storability of muscadine cultivars was SU, PA, AL, GV, TR, and HA. [ABSTRACT FROM AUTHOR]

The current research applied four methods of supplementary pollination from male flowers of kiwifruit (cv. Tomori). These methods were open pollination, hand pollination, spray pollination with pure pollen, and spray pollination with impure pollen, collected from the entrance of bee hives at the time of male kiwifruit flower opening and arranged in three replications in 2021. The fruits were harvestable after reaching the maturity stage (6.2 °Brix) and stored for 90 days at 0 °C. The results showed that kiwifruit decay (1.70%), weight loss (3.14%), total soluble solids (TSS) (149.62%), and TSS: titratable acidity (TA) ratio (TSS/TA) (333.04%) significantly (P ≤ 0.01) increased during 90 days of cold storage. However, fruit firmness (38.24%), TA (42.10%), total chlorophyll (53.72%), carotenoid (18.65%), vitamin C (10.34%), flavonoid (12.87%), and antioxidant activity (13.29%) significantly decreased through storage time. Furthermore, total phenolic content (TPC) increased substantially after 30 days of cold storage but then decreased. Generally, fruits from vines sprayed with impure pollen had higher firmness, bioactive compounds, and sensory attributes. They had lower decay and less weight loss after 90 days of cold storage. Overall, spraying kiwifruit vines with impure pollen could be recommended as a practical supplementary pollination in kiwifruits, cv. Hayward, regarding postharvest quality maintenance. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Methods Results Conclusions Machine perfusion is a promising strategy for safeguarding liver transplants donated after cardiac death (DCD). In this study, we developed and validated a novel machine perfusion approach for mitigating risk factors and salvaging severe DCD livers.A novel hypothermic oxygenated perfusion (HOPE) system was developed, incorporating two pumps and an elastic water sac to emulate the functionality of the cardiac cycle. Compared to conventional systems (HOPE S1 and S2), the novel HOPE system (HOPE S3) was evaluated in rats, utilizing healthy livers perfused with methylene blue diluted using Histidine‐tryptophan‐ketoglutarate (HTK) solution or DCD livers subjected to 60 min of warm ischemia without heparin administration. Liver perfusion outcomes were assessed through macroscopic and microscopic evaluations, molecular analyses, and orthotopic liver transplantation (OLT).DCD livers subjected to HOPE systems' perfusion exhibited decreased injury and enhanced survival rates compared to static cold storage following 60 min of warm ischemia (DCD + SCS). The 4‐week post‐transplantation survival rates were 0%, 20%, and 33% in the DCD + SCS, HOPE S1, and HOPE S2 groups, respectively. HOPE S3 conferred protection against hepatocyte and non‐parenchymal cell injury, resulting in a 67% animal survival rate following 60 min of warm donor ischemia (HOPE S3). Assessments of hepatic sinusoidal microcirculation, morphological changes, and molecular alterations in preserved livers further confirmed these findings.The newly devised machine perfusion system can enhance and uniform liver perfusion and may become a promising tool for revitalizing DCD liver grafts afflicted with severe warm ischemic injuries. [ABSTRACT FROM AUTHOR]

ABSTRACT Chicken thigh is a popular and widely consumed meat product. However, its high moisture content and susceptibility to microbial spoilage limit its shelf life. To address this issue, we investigated the efficacy of an edible coating based on alginate nanoparticles (AlgNPs) containing menthol, Zataria multiflora essential oil (EO), or their combination for extending the shelf life of chicken thigh. The nanoparticles were prepared through ionic gelation methods; their particle size was obtained as 87–205 nm with PDI values of < 0.3 and SPAN values of < 1. The AlgNPs containing both menthol and the EO showed the most potent antimicrobial activity (the lowest counts of pseudomonas, aerobic mesophilic, and yeast‐mold), during cold storage. This coating treatment showed also the highest antioxidant activities, with the lowest thiobarbituric acid reactive substances (TBARS), pH and total volatile basic nitrogen (TVBN) values. Moreover, this coating significantly (p < 0.05) showed the highest sensory quality of chicken thighs, as evidenced by the higher sensory scores for texture, odor, color, and overall acceptability. Therefore, the edible coating based on AlgNPs containing menthol and Z. multiflora EO is an effective and promising approach for extending the shelf life of chicken thighs. [ABSTRACT FROM AUTHOR]

Thermal energy storage (TES) systems open up alternative paths for air conditioning to increase the range of battery electric vehicles (BEVs) by reducing power consumption. The central prerequisites for this purpose are high storage densities: high-temperature TES systems are being focused on for heat demands, while effective solutions for cooling are missing. Due to their lower temperature potentials, concepts with high storage capacities and heat transports between the storage and cold transferring medium are needed. Latent TES systems based on water enable these capacities but require adequate internal structures for effective heat transfer. Due to the large number of geometric options, high simulation efforts must be conducted to identify favored structures, or the possible design space must be limited for investigations. For this purpose and for the first time, an alternative way is presented using newly developed dimensionless models in a top-down methodology for time-efficient design studies and evaluations. These models were successfully validated and used as a design tool to identify effective structures in latent TES systems for cooling demands in BEVs. A wide array of variation studies on tube, finned plate and novel Triply Periodic Minimal Surface (TPMS) structures were performed and uniformly evaluated with regard to storage densities, cooling efficiencies and geometry. The results show high storage densities for novel TPMS structures, including the enclosure of 100 Wh/kg or 102.2 kWh/m3 with average cooling capacities of 1 kW over 30 min, confirming the usability of latent TES systems in terms of compactness and efficiency for cooling demands in BEVs. [ABSTRACT FROM AUTHOR]

Background Methods Results Conclusion Production of platelet concentrates (PCs) involves several steps that significantly affect platelet behavior. To gain a deeper understanding of how storage conditions impact donor platelet recirculation and functionality post‐transfusion, ex vivo platelet labeling is a feasible approach. However, before pursuing clinical investigations of platelet recirculation and function in humans, we aimed to determine the effects of pathogen inactivation technology (PIT) and storage conditions (4°C vs. room temperature [RT]) on phenotype and function of biotinylated platelets compared to conventional PIT PCs for transfusion.Nine PCs were prepared in 61% additive solution from 45 buffy coats (five buffy coats each). A pool‐and‐split of three units was used to prepare three equivalent PCs: two labeled with biotin and stored at RT or 4°C, and one without labeling and stored at RT. All PCs were then treated by PIT (amotosalen/UVA) and stored for 14 days. Labeling efficiency, platelet concentration, metabolic parameters, aggregation response (ADP, collagen, co‐aggregation with epinephrine), and platelet phenotype (CD42b, CD62‐P, phosphatidylserine) at the basal stage and upon stimulation (ADP or TRAP‐6) were performed.Labeling efficiency of PIT and 4°C PCs was stable over 14 days of storage. Differences in platelet function and phenotype were mainly due to the storage temperature and not the biotinylation process. Phenotypes at baseline or after stimulation were equivalent in biotin‐positive and biotin‐negative platelets.Biotin‐labeled platelets can effectively enable investigation of the effects of PIT and storage temperature for clinical studies. This method shows great potential for improving platelet transfusion knowledge. [ABSTRACT FROM AUTHOR]

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The present study aims to evaluate the physicochemical and sensory characteristics of A2 yoghurts (made with A2A2 β-CN milk), in comparison with Control yoghurts (elaborated from conventional milk, a mixture of A1 and A2 β-CN milk). The pH, acidity, water-holding capacity, spontaneous syneresis, firmness and color of yoghurts were monitored during their cold storage (4 °C) for 35 days. Two independent sensory tests (with expert judges and consumers) were also performed. The A2 yoghurts showed only minor differences in some of their physicochemical and sensory characteristics compared to those made with conventional milk. At specific storage times, the A2 yoghurt exhibited higher levels of acidity, luminosity (L*) and firmness, compared to the Control. No differences were observed in the growth curves of the starter (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus) during the yoghurt production, nor in the water-holding capacity or spontaneous syneresis of the two types of gels. Regarding the sensory evaluation of samples, the A2 yoghurts were described as firmer and more adherent (by the expert panel), and brighter and more homogeneous (by the consumers) than the Control. In all cases, both consumers and expert sensory panels showed a preference for the A2 yoghurts. Therefore, these results demonstrate the suitability of A2A2 β-CN milk for producing yoghurts with similar characteristics to those obtained with conventional milk. [ABSTRACT FROM AUTHOR]

Harvesting cold energy from the universe by radiative cooling (RC) is a promising zero‐carbon route for green cooling. However, low power density and spatiotemporal energy mismatch of RC are great challenges for realizing efficient space cooling. Herein, a facile strategy for regulating cold energy from the universe by bifunctional phase change materials (PCM) for sustainable cooling is proposed. A bifunctional phase‐change composite film (PCCF) by integrating RC coating with PCM for 24‐h cold energy harvesting, storage, and utilization is demonstrated. The bifunctional PCCF can harvest cold energy from the universe and regulate the redundant cold energy generated by nighttime RC to compensate for the cold shortage of daytime RC, realizing flexible regulation of all‐day RC and setting new records of cooling power up to 180 W m−2 with sub‐ambient temperature drop of 11.95 °C. The work offers a promising strategy for zero‐carbon sustainable cooling by maximizing RC with cold energy storage. [ABSTRACT FROM AUTHOR]

Background Methods Results Conclusions Preclinical and clinical studies have demonstrated the advantages of oxygenated hypothermic machine perfusion (HMPO2) during kidney preservation. However, the optimal oxygenation levels during HMPO2 remain undetermined. The aim of this study was to compare different levels of oxygenation (0%, 21%, 50%, and 100%) during 22 h of active oxygenated HMP (HMPO2) using oxygen preloading by bubbling oxygenation in the preservation solution and continuous surface oxygenation during MP in a porcine DCD model.After 60 min of warm ischemia time, both kidneys of an 80 kg pig were procured and randomized to one of the following groups: (1) 22‐h static cold storage (n = 6), (2) 22‐h HMP without active oxygenation (n = 6), (3) 22‐h HMPO2 21% O2 (n = 6), (4) 22‐h HMPO2 50% O2 (n = 6), and (5) 22‐h HMPO2 100% O2 (n = 6). The primary outcome measure was to compare the different oxygen levels among the different groups assessed by cortex kidney tissue oxygen partial pressures (tpO2).Continuous HMPO2 resulted in a significant modification of cortex kidney tpO2. In addition, tpO2 was dependent on the percentage of oxygenation. One hundred percent oxygen resulted in a significantly higher tpO2 compared to all other study groups. In line with that, ATP resynthesis was significantly higher in the HMPO2 100% group.This study demonstrates that continuous HMPO2 results in a significant modification of tpO2 compared with SCS, and the degree of tpO2 is positively correlated with the percentage of active oxygenation during HMP. Metabolic profile analyses (ATP resynthesis) suggest that the aerobic mechanism is better supported with higher oxygen levels (50% and 100% oxygenation). [ABSTRACT FROM AUTHOR]

Chronic kidney disease (CKD) is a prevalent disease affecting almost 10% of the world's population, with many cases progressing to end-stage kidney disease (ESKD). Kidney transplantation (KT) is the gold-standard treatment for ESKD. Due to growing KT waitlists, the deceased kidney donor (DKDs) criteria have expanded to increase the number of available kidney grafts. Kidney graft preservation ensures optimal graft function after KT. Static cold storage (SCS) as a preservation method is still widely used. Hypothermic machine perfusion (HMP) has proven to decrease delayed graft function (DGF) and increase graft survival. Most recent studies advocate for the use of HMP regardless of donor type. However, emerging technologies, such as hypothermic oxygenated machine perfusion (HOPE) and normothermic machine perfusion (NMP), have shown promising results in specific scenarios. This review aims to provide a summary of the well-established kidney graft preservation methods and their outcomes, as well as novel technological advances that allow for newer preservation strategies. [ABSTRACT FROM AUTHOR]