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5. Kratom: An Emerging Issue for Research and Physician Education.

Author

Collar, Amanda L. and Barrett, Eileen D.

Subjects
*KRATOM, *PHYSICIANS, *EDUCATION research
Abstract

Kratom is legally available in most states and is increasingly used. The authors of this commentary highlight kratom's unregulated status and other gaps that physicians should be aware of in light of emerging evidence on its clinical risks. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Fine-mapping of human antibody specificity in response to natural Chlamydia trachomatis infection utilized to inform vaccines against adhesion factors

Author

Kathryn M. Frietze, PhD, Bryce Chackerian, PhD, Judy Cannon, PhD, Thomas Byrd, MD, Collar, Amanda L, Kathryn M. Frietze, PhD, Bryce Chackerian, PhD, Judy Cannon, PhD, Thomas Byrd, MD, and Collar, Amanda L

Subjects
chlamydia
Abstract

Chlamydia trachomatis (Ct) is the causative pathogen for the most common bacterial sexually transmitted infection worldwide and can cause serious medical consequences in women, including pelvic inflammatory disease, ectopic pregnancy, and infertility. For these reasons, a Ct vaccine is urgently needed, yet, its development remains a significant challenge. One barrier to producing a Ct vaccine is the gap in knowledge of protective immune responses for Ct infection, including the role that antibodies may play. Therefore, I aimed to characterize the human antibody response to natural urogenital Ct infection in women using Deep Sequence-Coupled Biopanning. Further, I leveraged these findings to engineer novel Ct vaccines targeting epitopes of three adhesion factors [the Major Outer Membrane Protein (MOMP), outer membrane complex protein B (OmcB), and polymorphic membrane protein D (PmpD)] using the bacteriophage Qb virus-like particle vaccine platform. I found that these vaccines were efficacious via in vitro assays to investigate antibody functionality and in vivo murine challenge models.

Published
2022

10. Annals On Call - Kratom: A Substance Physicians Should Be Aware Of.

Author

Centor, Robert M., Barrett, Eileen D., and Collar, Amanda L.

Subjects
KRATOM, PHYSICIANS, SUBSTANCE abuse
Abstract

In this episode of Annals On Call, Dr. Centor discusses kratom and what physicians should know about how patients may be using this substance with Drs. Eileen Barrett and Amanda Collar. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Immunogenicity and Protective Capacity of a Virus-like Particle Vaccine against Chlamydia trachomatis Type 3 Secretion System Tip Protein, CT584

Author

Webster, Everett, primary, Seiger, Kyra W., additional, Core, Susan B., additional, Collar, Amanda L., additional, Knapp-Broas, Hannah, additional, Graham, June, additional, Shrestha, Muskan, additional, Afzaal, Sarah, additional, Geisler, William M., additional, Wheeler, Cosette M., additional, Chackerian, Bryce, additional, Frietze, Kathryn M., additional, and Lijek, Rebeccah S., additional

Published
2022
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13. Sexual Enrichment Aids: A Mixed Methods Study Evaluating Use, Hygiene, and Risk Perception among Women.

Author

Collar, Amanda L., Fuentes, Jesus E., Rishel Brakey, Heidi, and Frietze, Kathryn M.

Subjects
*SEX toys, *SEXUAL partners, *SEXUAL health, *HYGIENE, *SEX education
Abstract

Sexual Enrichment Aids (SEAs), or "sex toys" like dildos or vibrators, are used to enrich sexual experiences, either alone or with sexual partners. Although SEA use has become increasingly prevalent in recent decades, there remain significant gaps in knowledge regarding sexual behaviors and hygiene surrounding their use. In this study, we use mixed methods approaches (cross-sectional survey of n = 800 women and qualitative semi-structured interviews of n = 24 women) to better understand sexual behavior, potential risks, and hygiene practices of women who self-identify as having sex with men, with women, or with women and men when using SEAs. We identified SEA use is common, with 79.9% of women using an SEA. Among these women, 31.8% of women indicated that they share SEAs with sexual partners. Further, condom use is rare while sharing SEAs, with only 14% of women utilizing condoms regularly. The majority (81.8%) of women wash their SEAs, primarily with soap and water. Yet, there is no consensus among women interviewed regarding the perceived risk associated with SEA use. Together, our findings support the need for increased evidence-based education for women to increase safety and hygiene of SEA use. [ABSTRACT FROM AUTHOR]

Published
2022
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15. A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex

Author

Yang, Desirée C., Blair, Kris M., Taylor, Jennifer A., Petersen, Timothy W., Sessler, Tate, Tull, Christina M., Leverich, Christina K., Collar, Amanda L., Wyckoff, Timna J., Biboy, Jacob, Vollmer, Waldemar, and Salama, Nina R.

Subjects
Bacterial Outer Membrane, Bacterial Proteins, Helicobacter pylori, Cell Wall, Endopeptidases, Mutation, Cytoskeleton, Genome, Bacterial, Research Article
Abstract

Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering properties measured by flow cytometry. Profiling of an arrayed genome-wide deletion library identified 28 genes that influence different aspects of cell shape, including properties of the helix, cell length or width, cell filament formation, cell shape heterogeneity, and cell branching. Included in this mutant collection were two that failed to form any helical cells, a soluble lytic transglycosylase and a previously uncharacterized putative multipass inner membrane protein HPG27_0728, renamed Csd7. A combination of cell fractionation, mutational, and immunoprecipitation experiments show that Csd7 and Csd2 collaborate to stabilize the Csd1 peptidoglycan (PG) endopeptidase. Thus, both csd2 and csd7 mutants show the same enhancement of PG tetra-pentapeptide cross-linking as csd1 mutants. Csd7 also links Csd1 with the bactofilin CcmA via protein-protein interactions. Although Csd1 is stable in ccmA mutants, these mutants show altered PG tetra-pentapeptide cross-linking, suggesting that Csd7 may directly or indirectly activate as well as stabilize Csd1. These data begin to illuminate a highly orchestrated program to regulate PG modifications that promote helical shape, which includes nine nonessential nonredundant genes required for helical shape and 26 additional genes that further modify H. pylori’s cell morphology. IMPORTANCE The stomach ulcer and cancer-causing pathogen Helicobacter pylori has a helical cell shape which facilitates stomach infection. Using light scattering to measure perturbations of cell morphology, we identified 28 genes that influence different aspects of cell shape. A mutant in a previously uncharacterized protein renamed Csd7 failed to form any helical cells. Biochemical analyses showed that Csd7 collaborates with other proteins to stabilize the cell wall-degrading enzyme Csd1. Csd7 also links Csd1 with a putative filament-forming protein via protein-protein interactions. These data suggest that helical cell shape arises from a highly orchestrated program to regulate cell wall modifications. Targeting of this helical cell shape-promoting program could offer new ways to block infectivity of this important human pathogen.

Published
2019

16. Aspergillosis, eosinophilic esophagitis, and allergic rhinitis in signal transducer and activator of transcription 3 haploinsufficiency

Author

Natarajan, Mukil, Hsu, Amy P., Weinreich, Michael A., Zhang, Yuan, Niemela, Julie E., Butman, John A., Pittaluga, Stefania, Sugui, Janyce, Collar, Amanda L., Lim, Jean K., Zangeneh, Tirdad, Carr, Tara, Oler, Andrew J., Similuk, Morgan, Rosen, Lindsey B., Desai, Jigar V., Freeman, Alexandra F., Holland, Steven M., Kwon-Chung, Kyung J., Milner, Joshua D., and Lionakis, Michail S.

Published
2018
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17. A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex

Author

Yang, Desirée C., primary, Blair, Kris M., additional, Taylor, Jennifer A., additional, Petersen, Timothy W., additional, Sessler, Tate, additional, Tull, Christina M., additional, Leverich, Christina K., additional, Collar, Amanda L., additional, Wyckoff, Timna J., additional, Biboy, Jacob, additional, Vollmer, Waldemar, additional, and Salama, Nina R., additional

Published
2019
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18. Microglia activation in Niemann–Pick disease, type C1 is amendable to therapeutic intervention

Author

Cougnoux, Antony, primary, Drummond, Rebecca A, additional, Collar, Amanda L, additional, Iben, James R, additional, Salman, Alexander, additional, Westgarth, Harrison, additional, Wassif, Christopher A, additional, Cawley, Niamh X, additional, Farhat, Nicole Y, additional, Ozato, Keiko, additional, Lionakis, Michail S, additional, and Porter, Forbes D, additional

Published
2018
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19. The homozygous CX3CR1-M280 mutation impairs human monocyte survival

Author

Collar, Amanda L., primary, Swamydas, Muthulekha, additional, O’Hayre, Morgan, additional, Sajib, Md Sanaullah, additional, Hoffman, Kevin W., additional, Singh, Satya P., additional, Mourad, Ahmad, additional, Johnson, Melissa D., additional, Ferre, Elise M.N., additional, Farber, Joshua M., additional, Lim, Jean K., additional, Mikelis, Constantinos M., additional, Gutkind, J. Silvio, additional, and Lionakis, Michail S., additional

Published
2018
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22. Production and Purification of Filovirus Glycoproteins in Insect and Mammalian Cell Lines

Author

Clarke, Elizabeth C., primary, Collar, Amanda L., additional, Ye, Chunyan, additional, Caì, Yíngyún, additional, Anaya, Eduardo, additional, Rinaldi, Derek, additional, Martinez, Britney, additional, Yarborough, Sarah, additional, Merle, Christine, additional, Theisen, Manfred, additional, Wada, Jiro, additional, Kuhn, Jens H., additional, and Bradfute, Steven B., additional

Published
2017
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23. Extrapulmonary Aspergillus infection in patients with CARD9 deficiency

Author

Rieber, Nikolaus, primary, Gazendam, Roel P., additional, Freeman, Alexandra F., additional, Hsu, Amy P., additional, Collar, Amanda L., additional, Sugui, Janyce A., additional, Drummond, Rebecca A., additional, Rongkavilit, Chokechai, additional, Hoffman, Kevin, additional, Henderson, Carolyn, additional, Clark, Lily, additional, Mezger, Markus, additional, Swamydas, Muthulekha, additional, Engeholm, Maik, additional, Schüle, Rebecca, additional, Neumayer, Bettina, additional, Ebel, Frank, additional, Mikelis, Constantinos M., additional, Pittaluga, Stefania, additional, Prasad, Vinod K., additional, Singh, Anurag, additional, Milner, Joshua D., additional, Williams, Kelli W., additional, Lim, Jean K., additional, Kwon-Chung, Kyung J., additional, Holland, Steven M., additional, Hartl, Dominik, additional, Kuijpers, Taco W., additional, and Lionakis, Michail S., additional

Published
2016
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24. Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy

Author

Ferre, Elise M.N., primary, Rose, Stacey R., additional, Rosenzweig, Sergio D., additional, Burbelo, Peter D., additional, Romito, Kimberly R., additional, Niemela, Julie E., additional, Rosen, Lindsey B., additional, Break, Timothy J., additional, Gu, Wenjuan, additional, Hunsberger, Sally, additional, Browne, Sarah K., additional, Hsu, Amy P., additional, Rampertaap, Shakuntala, additional, Swamydas, Muthulekha, additional, Collar, Amanda L., additional, Kong, Heidi H., additional, Lee, Chyi-Chia Richard, additional, Chascsa, David, additional, Simcox, Thomas, additional, Pham, Angela, additional, Bondici, Anamaria, additional, Natarajan, Mukil, additional, Monsale, Joseph, additional, Kleiner, David E., additional, Quezado, Martha, additional, Alevizos, Ilias, additional, Moutsopoulos, Niki M., additional, Yockey, Lynne, additional, Frein, Cathleen, additional, Soldatos, Ariane, additional, Calvo, Katherine R., additional, Adjemian, Jennifer, additional, Similuk, Morgan N., additional, Lang, David M., additional, Stone, Kelly D., additional, Uzel, Gulbu, additional, Kopp, Jeffrey B., additional, Bishop, Rachel J., additional, Holland, Steven M., additional, Olivier, Kenneth N., additional, Fleisher, Thomas A., additional, Heller, Theo, additional, Winer, Karen K., additional, and Lionakis, Michail S., additional

Published
2016
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25. CXCR1-mediated neutrophil degranulation and fungal killing promote Candida clearance and host survival

Author

Swamydas, Muthulekha, primary, Gao, Ji-Liang, additional, Break, Timothy J., additional, Johnson, Melissa D., additional, Jaeger, Martin, additional, Rodriguez, Carlos A., additional, Lim, Jean K., additional, Green, Nathaniel M., additional, Collar, Amanda L., additional, Fischer, Brett G., additional, Lee, Chyi-Chia Richard, additional, Perfect, John R., additional, Alexander, Barbara D., additional, Kullberg, Bart-Jan, additional, Netea, Mihai G., additional, Murphy, Philip M., additional, and Lionakis, Michail S., additional

Published
2016
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26. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System

Author

Drummond, Rebecca A., primary, Collar, Amanda L., additional, Swamydas, Muthulekha, additional, Rodriguez, Carlos A., additional, Lim, Jean K., additional, Mendez, Laura M., additional, Fink, Danielle L., additional, Hsu, Amy P., additional, Zhai, Bing, additional, Karauzum, Hatice, additional, Mikelis, Constantinos M., additional, Rose, Stacey R., additional, Ferre, Elise M. N., additional, Yockey, Lynne, additional, Lemberg, Kimberly, additional, Kuehn, Hye Sun, additional, Rosenzweig, Sergio D., additional, Lin, Xin, additional, Chittiboina, Prashant, additional, Datta, Sandip K., additional, Belhorn, Thomas H., additional, Weimer, Eric T., additional, Hernandez, Michelle L., additional, Hohl, Tobias M., additional, Kuhns, Douglas B., additional, and Lionakis, Michail S., additional

Published
2015
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27. Fine-mapping of human antibody specificity in response to natural Chlamydia trachomatis infection utilized to inform vaccines against adhesion factors

Author

Collar, Amanda L

Subjects
chlamydia, vaccine, virus-like particle, sexually transmitted infection, vaccinology, antibody, Immunology of Infectious Disease
Abstract

Chlamydia trachomatis (Ct) is the causative pathogen for the most common bacterial sexually transmitted infection worldwide and can cause serious medical consequences in women, including pelvic inflammatory disease, ectopic pregnancy, and infertility. For these reasons, a Ct vaccine is urgently needed, yet, its development remains a significant challenge. One barrier to producing a Ct vaccine is the gap in knowledge of protective immune responses for Ct infection, including the role that antibodies may play. Therefore, I aimed to characterize the human antibody response to natural urogenital Ct infection in women using Deep Sequence-Coupled Biopanning. Further, I leveraged these findings to engineer novel Ct vaccines targeting epitopes of three adhesion factors [the Major Outer Membrane Protein (MOMP), outer membrane complex protein B (OmcB), and polymorphic membrane protein D (PmpD)] using the bacteriophage Qb virus-like particle vaccine platform. I found that these vaccines were efficacious via in vitro assays to investigate antibody functionality and in vivo murine challenge models.

Published
2022

28. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System

Author

Lionakis, Michail S., Datta, Sandip K., Chittiboina, Prashant, Karauzum, Hatice, Fink, Danielle L., Rosenzweig, Sergio D., Drummond, Rebecca A., Weimer, Eric T., Kuhns, Douglas B., Lim, Jean K., Mendez, Laura M., Belhorn, Thomas H., Yockey, Lynne, Ferre, Elise M. N., Mikelis, Constantinos M., Lemberg, Kimberly, Hernandez, Michelle L., Hsu, Amy P., Collar, Amanda L., Rose, Stacey R., Lin, Xin, Rodriguez, Carlos A., Hohl, Tobias M., Zhai, Bing, Kuehn, Hye Sun, and Swamydas, Muthulekha

Subjects
3. Good health
Abstract

Candida is the most common human fungal pathogen and causes systemic infections that require neutrophils for effective host defense. Humans deficient in the C-type lectin pathway adaptor protein CARD9 develop spontaneous fungal disease that targets the central nervous system (CNS). However, how CARD9 promotes protective antifungal immunity in the CNS remains unclear. Here, we show that a patient with CARD9 deficiency had impaired neutrophil accumulation and induction of neutrophil-recruiting CXC chemokines in the cerebrospinal fluid despite uncontrolled CNS Candida infection. We phenocopied the human susceptibility in Card9-/- mice, which develop uncontrolled brain candidiasis with diminished neutrophil accumulation. The induction of neutrophil-recruiting CXC chemokines is significantly impaired in infected Card9-/- brains, from both myeloid and resident glial cellular sources, whereas cell-intrinsic neutrophil chemotaxis is Card9-independent. Taken together, our data highlight the critical role of CARD9-dependent neutrophil trafficking into the CNS and provide novel insight into the CNS fungal susceptibility of CARD9-deficient humans.

29. CXCR1-mediated neutrophil degranulation and fungal killing promote Candidaclearance and host survival

Author

Swamydas, Muthulekha, Gao, Ji-Liang, Break, Timothy J., Johnson, Melissa D., Jaeger, Martin, Rodriguez, Carlos A., Lim, Jean K., Green, Nathaniel M., Collar, Amanda L., Fischer, Brett G., Lee, Chyi-Chia Richard, Perfect, John R., Alexander, Barbara D., Kullberg, Bart-Jan, Netea, Mihai G., Murphy, Philip M., and Lionakis, Michail S.

Abstract

CXCR1 as a mediator of neutrophil fungal killing and host defense against systemic fungal infection in mice and humans.

Published
2016
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