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Atxurra Cave (Berriatua, Northern Spain) contains an important Magdalenian archaeological assemblage comprising evidence of ephemeral occupation in the lower cave level and a unique assemblage of rock art. With the aim of understanding the palaeoenvironmental conditions and the nature of the human occupation, detailed micromorphological, mineralogical, palynological and anthracological analyses were undertaken near the entrance of the cave. The lower part of the excavated sequence comprises almost unfossiliferous silty units deposited over a thick speleothem deposit, which alternates with a sandy-silt matrix-supported microfacies dated about 33.4 kyr B.P. Palynological assemblage in this unit suggest regional vegetation was formed by montane pines, junipers and broadleaved elements like birch and hazel. Overlying units of brown clayey material, dated to ca. 19.7–18.4 kyr B.P., contain evidence of a Gravettian occupation with Noailles burins, and a palynoflora indicative of localized conifers with a dense herbaceous steppe understory, in an arid and cold climate. At ca. 15.5–14.5 kyr B.P. there is a return to meso-thermophilous tree and shrub taxa within a warmer and humid climate background, which may have favored the regional development of the Magdalenian culture. The uppermost unit, dated ca. 7.7–7 kyr B.P., reveals postglacial expansion of a mixed Atlantic forest with no signals of anthropization before the start of the Neolithic.

Scores in standardized international student achievement tests and some recent adult literacy studies provide interesting data on the quality of educational outputs and on the skill level of the population that can be a useful complement to the data on the quantity of schooling which have been most commonly used in the growth literature. This paper describes the most recent available primary data on the subject, reviews different attempts to organize, standardize, and summarize them, and discusses the strengths and weaknesses of the existing indicators and their potential usefulness as explanatory variables in empirical analyses of the determinants of income and welfare levels and growth rates. A final section investigates the distribution of these indicators across a sample of 21 OECD countries.

Trace metal contents and fluxes in downward particulate matter and dated sediment cores of the NW Alboran Sea are analysed in this study with the aim of assessing the role of the Atlantic inflow on their transport. Increases in Zn, Cu and Pb were detected in downward particulate matter collected by sediment traps after river flooding events and after the Aznalcollar mining spill. Their arrival coincided within the recently estimated time range for river particles discharged into the Gulf of Cádiz to reach the Alboran Sea, indicating that their transfer is enhanced during events of increased river inputs of contaminated particulate matter. This also suggests that the effects of potential tailing dam failures in the Gulf of Cádiz watersheds could reach the Alboran Sea. These trace metals also increased in the sediment cores from the continental rise since the second half of the 19th century, suggesting that contaminated particles have been continuously transferred towards the Mediterranean Sea since that time, when mining concessions and production increased in the SW Iberian Pyrite Belt

The European eel (Anguilla anguilla) is a critically endangered species. The impact of environmental contamination on this species has been highlighted as contributing to the decline in recruitment. The Mar Menor hypersaline coastal lagoon (SE Spain) is one of the most productive fsheries of European eel in Europe, making it a critical habitat for species conservation. The present study aimed to provide an initial overview of the impact of organic chemical contaminants on the European eel and the potential sublethal efects of chemical pollution on pre-migrating eels in this hypersaline habitat. We investigated muscle bioaccumulation of main persistent and hazardous organic contaminants (including some current-use pesticides) and genotoxicity, neurotoxicity, and xenobiotic detoxifcation system responses. The fndings show that lagoon eels were exposed to high levels of legacy organochlorine contaminants, recently banned pesticides (chlorpyrifos), and some emerging chemicals. Some individuals surpassed the maximum levels of CBs authorized by the European Commission for human consumption. In this species, residuals of chlorpyrifos, pendimethalin, and chlorthal dimethyl have been reported for the first time. This feld study provides relevant data to stock management and human health consumption and provides the frst biomarker responses in European eel under permanent hypersaline conditions. Furthermore, the high frequency of micronuclei in peripheral erythrocytes of lagoon eels indicates sublethal genotoxic efects on the organism. Overall, the European eels growing and maturing in the Mar Menor lagoon are exposed to toxic and carcinogenic chemicals. The lack of seafood safety regulations for human consumption for some legacy chemicals that were measured in high concentrations in our study requires special action. Further biomonitoring and research are recommended to protect the animal, public, and environmental health

A coupled physical (Regional Ocean Modeling System, ROMS)-biogeochemical model (N2PZD2) at a horizontal resolution of 3.5 km was implemented for N and NW Iberia, an area of high productivity associated with upwelling. The physical model has been the object of previous studies and has proven its capability to perform well in reproducing the main oceanographic features in the area (upwelling, river plumes, slope currents, fronts, filaments), which is fundamental to properly representing the variability and distribution of the biogeochemical variables. The biogeochemical model was set up to account for the main nutrient inputs in the area: upwelling and rivers. Upwelling input required proper characterization of the nutrient content variability of the Eastern North Atlantic Central Water, which was achieved by using a temperature-nitrate relationship obtained from observations to impose nitrate at the open boundaries. The resulting biophysical model accurately reproduced the timing and interannual variability of the spring bloom compared with satellite chlorophyll (chl a) observations. A comparison with the Instituto Español de Oceanografía’s in situ spring-monitoring Pelacus cruises (which include plankton) revealed that the model was able to reproduce the variability at shorter scales (days) and demonstrated its ability to complement the observational data and reveal the variability in the area around the spring transition. In this respect, both the model and observations showed that productivity on this narrow shelf is affected by seasonal upwelling that results from the interplay of wind, river plumes and light intensity, all varying at interannual, seasonal and event scales.

A central issue in avian ecology deals with the trade-off between investment life-history components, such as reproductive effort, and parasite and disease resistance. During reproduction, differences in the particular needs of males and females may further affect the outcome of such trade-off. However, most studies performed to date on avian species have focused on males, while less is known about this subject in females. We investigated haemoparasite infections (genera Haemoproteus, Plasmodium and Leucocytozoon) in relation to sex, year, body condition and plasma concentrations of carotenoids in wild-breeding Red-legged partridges (Alectoris rufa). Our aim was to examine whether there are differences in infections between sexes and how parasite infections relate to carotenoid levels, physical condition and breeding parameters in non-passerine wild birds. Males captured early in the breeding season were in better body condition than females, indicating a marked sexual difference in this trait in wild Red-legged partridges. The prevalence of blood parasites in males was higher than in females. However, we found that females infected by blood parasites had lower plasma carotenoid concentrations than uninfected females, whereas no association between infection and carotenoid levels was found in males. This suggests sex-related differences in the use of carotenoids to fight infections or for parasite resistance. A possible explanation of this contrasting pattern between sexes is that reproduction may not have to involve the same costs for males and females. We suggest that males would be under strong sexual selection and would trade health for signalling, which could simultaneously explain highest parasite prevalence being found in males and the contrasting patterns in blood carotenoid levels between males and females. Females in contrast, that were in worst body condition during early breeding season, were more sensitive to infections, diverting carotenoids to immune

Drought monitoring systems are real-time information systems focused on drought severity data. They are useful for determining the drought onset and development and defining the spatial extent of drought at any time. Effective drought monitoring requires databases with high spatial and temporal resolution and large spatial and temporal coverage. Recent reanalysis datasets meet these requirements and offer an excellent alternative to observational data. In addition, reanalysis data allow better quantification of some variables that affect drought severity and are more seldom observed. This study presents a global drought dataset and a monitoring system based on the Standardized Precipitation Evapotranspiration Index (SPEI) and ERA5 reanalysis data. Computation of the atmospheric evaporative demand for the SPEI follows the FAO-56 Penman-Monteith equation. The system is updated weekly, providing near real-time information at a 0.5° spatial resolution and global coverage. It also contains a historical dataset with the values of the SPEI at different time scales since January 1979. The drought monitoring system includes the assessment of drought severity for dominant crop-growing areas. A comparison between SPEI computed from the ERA5 and CRU datasets shows generally good spatial and temporal agreement, albeit with some important differences originating mainly from the different spatial patterns of SPEI anomalies, as well as from employing long-term climate trends for different regions worldwide. The results show that the ERA5 dataset offers robust results and supports its use for drought monitoring. The new system and dataset are publicly available at the link https://global-drought-crops.csic.es/.

Blueberries (Vaccinium corymbosum L.) are becoming increasingly popular for their nutritional and health benefits, and their economic value is therefore increasing. The loss of quality that can occur due to softening and fungal attack is an important consideration when marketing blueberries. Despite the added value of blueberries, no studies have been carried out on how the fruit arrives at the outlets just before purchase by the consumer in terms of firmness, physico-chemical parameters, phenolic compounds, and fungal growth. The aim of this work has been, therefore, to investigate possible differences in quality parameters between blueberries purchased from ten different outlets, regardless of the supplier. The results showed that all the samples were of acceptable quality, although they all had a low maturity index at the point of sale. None of the samples studied showed clear signs of fungal decay at the time of purchase, although we were able to grow and identify some pathogen specimens after cultivation. In terms of total phenolic and anthocyanin content, as well as antioxidant activity, all the samples showed low values, possibly due to their postharvest storage, but they were within the expected range for this fruit. On the other hand, differences in the measured parameters were observed between samples of the same cultivar while no differences were found between conventionally and organically grown blueberries. This suggests that preharvest (such as edaphoclimatic conditions, agricultural practices, and cultivars) and postharvest factors (such as treatments used, storage, and transport temperatures) could influence the berry quality when they reach the consumer.

Coastal lagoons are among the most productive and biodiverse systems in the world and are important sentinels of climate change. The Mar Menor is one of the largest coastal lagoons in the Mediterranean, providing a variety of ecosystem services and resources to the community. However, in recent decades this lagoon has suffered drastic changes and degradation caused by human activities. We analyzed the concentration of dissolved organic carbon (DOC) and the optical properties of dissolved organic matter (DOM) in the water column and sediment pore water during the summer and winter of 2018 and during eighteen months from 2016 to 2018. Overall, we found that the composition of DOM is mainly related to and enhanced by anthropogenic activities and microbial metabolism. DOM enters the lagoon via urban and agricultural runoff, drainage systems, and wastewater treatment plants. Additionally, strong microbial metabolism in sediments leads to differences in DOM composition between water and sediments. In the water column, humic-like components accounted for 71 % of the total DOM, while protein-like compounds were most abundant in sediment pore water. We observed a strong seasonal variability associated with precipitation and the system collapse in 2016 (phytoplankton bloom), which resulted in the death of 80 % of macrophytes. The sediments act as a source of DOM to the overlying water, likely due to relatively high organic matter content and intense microbial activity, primarily through anaerobic pathways. Benthic fluxes of DOC ranged from 5.24 to 33.30 mmol m−2 d−1, being higher in winter than summer 2018 and decreasing from north to south, likely related to lower residence time in the northern basin, groundwater discharge and accumulation of organic matter from the dead meadows. We estimate a net flux of DOC from the Mar Menor toward the Mediterranean Sea of 1.57 × 107 mol yr−1

Cultivated bread wheat (Triticum aestivum L.) spontaneously hybridizes with wild/weedy related Aegilops populations, but little is known about the actual rates at which this hybridization occurs under field conditions. It is very important to provide reliable empirical data on this phenomenon in order to assess the potential crop-wild introgression, especially in the context of conducting risk assessments for the commercialization of genetically modified (GM) wheat, as gene flow from wheat to Aegilops species could transfer into the wild species genes coding for traits such as resistance to herbicides, insects, diseases or environmental stresses.

Ongoing ocean warming due to climate change poses new challenges for marine life and its exploitation. We have used transcriptomics to find genetically based responses to increased temperature in natural populations of the marine clam Ruditapes decussatus, which lives along parallel thermal gradients in southern Europe. Clams of the Atlantic and West Mediterranean races were collected in northern (cool) and a southern (warm) localities. The animals were kept in running seawater in the warm, southern Atlantic locality for a 15-week period. During this period, water temperature was raised to typical southern European summer values. After this period, an expression profile was obtained for a total of 34 clams and 11,025 probes by means of an oligonucleotide microarray. We found distinct transcriptional patterns for each population based on a total of 552 differentially expressed genes (DEGs), indicating innate differences which probably have a genetic basis. Race and latitude contributed significantly to gene expression differences, with very different sets of DEGs. A gene ontology analysis showed that races differed mainly in the genes involved in ribosomal function and protein biosynthesis, while genes related to glutathione metabolism and ATP synthesis in the mitochondria were the most outstanding with respect to north/south transcriptional differences.

Deception Island is the most active volcano in the South Shetland Islands with more than 20 explosive eruptions registered over the past centuries. The latest event (1970) was severely violent with a volcanic explosivity index (VEI) of 3. The column height reached 10 km, the estimated bulk eruptive volume was > 0.1 km3 and tephra fallout was reported as far as in King George Island (> 150 km distance). In this work, we perform a compositional and textural analysis of the 1970 tephra layers found at Livingston Island¿s glaciers. Results obtained are compared to the in situ pyroclastic deposits of the active vents during the eruption. The objective is to establish a correlation between the eruptive phases occurred during the 1970 event and the physicochemical features observed in the tephra deposits. This will be used the onset of future studies of tephra layers found in glaciers and marine/lacustrine sediment cores outside the island, improving our capacity to reconstruct the eruptive dynamics of past eruptions. This is fundamental to: (i) determine the size and explosiveness of past eruptions; (ii) assess the extent of their related hazards; (iii) complete the eruptive record; and (iv) consequently, perform accurate hazard assessments at the island.

Deception Island is the most active volcano in the South Shetland Islands (Antarctica) with more than 20 explosive eruptive events registered over the past few centuries. The most recent eruption (August 1970) was severely violent with a volcanic explosivity index (VEI) of 3. The column height reached 10 km at its maximum, the estimated bulk eruptive volume was > 0.1 km3 and tephra fallout was reported as far as in King George Island (> 150 km distance). In this work, we perform a compositional and textural analysis of the 1970 tephra layers found at Livingston Island¿s glaciers (between 25 and 40 km distance from Deception IsIand). Results obtained are then compared to the in situ pyroclastic deposits of the diverse vents active during this eruption. The objective is to establish a correlation between the eruptive phases occurred during the 1970 event and the physicochemical features observed in the tephra deposits. This will be used as a starting point for future studies of tephra layers found in glaciers and marine/lacustrine sediment cores outside the island, improving our capacity to reconstruct the eruptive dynamics of past eruptions. These results are fundamental to: (i) determine the size and explosiveness of past eruptive events; (ii) assess the extent of their related hazards (e.g., ash fall out); (iii) complete the eruption record of the island; and (iv) consequently, perform more accurate hazard assessments at the island. This is of special concern, since the South Shetland Islands are an important tourist destination and host numerous year-round and seasonal scientific stations and base camps.

Microalgae constitute a highly diverse group of photosynthetic microorganisms that are widely distributed on Earth. The rich diversity of microalgae arose from endosymbiotic events that took place early in the evolution of eukaryotes and gave rise to multiple lineages including green algae, the ancestors of land plants. In addition to their fundamental role as the primary source of marine and freshwater food chains, microalgae are essential producers of oxygen in the planet and a major biotechnological target for sustainable biofuel production and CO2 mitigation. Microalgae integrate light and nutrient signals to regulate cell growth. Recent studies identified the target of rapamycin (TOR) kinase as central regulator of cell growth and nutrient sensor in microalgae. TOR promotes protein synthesis and regulates processes that are induced under nutrient stress such as autophagy and the accumulation of triacylglycerol and starch. A detailed analysis of representative genomes from the entire microalgal lineage revealed the high conservation of central components of the TOR pathway likely present in the last eukaryotic common ancestor and the loss of specific TOR signaling elements at an early stage in the evolution of microalgae. Here we examine the evolutionary conservation of TOR signaling components in diverse microalgae and discuss recent progress on the study of this signaling pathway in these organisms.

Short-term gaseous treatments improve rachis quality during table grape postharvest, but little is known about the mechanisms involved. In this work, we observed that the application of a 3-day CO2 treatment at 0 °C improved rachis browning of Superior Seedless and Red Globe bunches, affecting the non-enzymatic antioxidant system by reducing the total phenolic content, the antioxidant activity and the expression of different stilbene synthase genes. Lipid peroxidation levels revealed lower oxidative stress in CO2-treated rachis of both cultivars linked to the activation of the enzymatic antioxidant system. Furthermore, whereas a positive correlation was denoted between rachis browning and the accumulation of key ABA regulatory genes in Red Globe bunches, this effect was restricted to ACS1, a key synthetic ethylene gene, in Superior Seedless clusters. This work also corroborated the important role of ethylene-responsive factors in the beneficial effect of the gaseous treatment, not only in the berries but also in the rachis. Finally, the application of the gaseous treatment avoided the induction of cell wall-degrading enzyme-related genes in both cultivars, which could favor the maintenance of rachis quality. This work provides new insight into specific responses modulated by the gaseous treatment focused on mitigating rachis browning independently of the cultivar.

In the original article, we neglected to include some funder information. The corrected ACKNOWLEDGMENTS section should be...

Estuarine sediments constitute a biogeochemical reservoir where chemical trace elements from natural sources mix with a fraction generated from human activities. The study of contamination events from sediment cores, when coupled with geochronological techniques, is useful for reconstructing the temporal evolution of chemical contamination in estuaries and for calculating trace elements (TE) fluxes. In particular, this can be applied to the “O Burgo” estuary in the Ria of “A Coruña”, where a phosphate fertilizers factory –currently dismantled- was settled and on which no study has been carried out until now. This estuary also has received sewage urban discharges. Then, it is hypothesized that the impact of this past anthropogenic activity, both urban and industrial, should be recorded in its estuarine sedimentary record. So, the rise and fall of these kind of anthropogenic pressures are addressed from three key questions: (i) identifying the chemical contamination in these sediments; (ii) temporally framing the contamination events by their chemical imprint on sediments; and (iii) defining the current situation by quantifying the lithogenic and anthropogenic fluxes to the sediment reservoir. In order to achieve these three objectives, one sediment core of 51 cm long was taken with a hand-driven Gouge Augers Sampler (43,31394°N, -8,35930°W) in the intertidal muddy area of this estuary. 210Pb, 214Pb and 137Cs activities were measured by low-level background gamma-ray spectrometry, and the Constant Rate of Supply model was applied for core dating (recovered sediments span the last 76±13 years). Major and trace elements were analysed by FAAS (Al, Ca and Fe), ETAAS (Hg) and ICP-MS (As, Cd, Co, Cr, Cu, lanthanoids -except Pm-, Mn, Mo, Ni, Pb, Th, U, V, Y and Zn). In addition, particulate organic matter proxies (C and N) were determined using an elemental CNH analyser. Elemental depth profiles showed high organic matter contents in the first 13 cm (3.2-4.0% of POC, and 0.2

The Strait of Gibraltar (SG) is the only connection of the Mediterranean Sea with the global circulation. The SG is an outstanding marine region to explore physical-biological coupling of pelagic communities due to its hydrodynamic complexity, including strong tidal forcing and marked spatial gradients and fronts. The authors have unravelled the role of the fortnightly tidal scale (spring and neap tides) and local processes (upwelling and tidal-topographic mixing) that shape planktonic assemblages in the Strait. To do so, an oceanographic cruise was taken in early autumn 2008 with a high-resolution grid sampling and spring/neap tidal conditions. The planktonic features were captured using different automatic and semi-automatic techniques of plankton analyses (flow cytometry, FlowCAM, LOPC and Ecotaxa) that allowed covering a wide range of sizes of the community from pico- to mesoplankton. The SG was sectorized into two clusters based on the biogeochemical and main water column properties. Cluster 1 (CL1) covered shallow productive areas around Cape Trafalgar (CT). CL1 presented higher concentrations of chlorophyll and nutrients, and phytoplankton was mostly represented by Synechococcus and coastal diatoms while zooplankton had the highest percentage of meroplankton (31%). In contrast, cluster 2 (CL2) covered open ocean waters and presented more oligotrophic features, i.e. nitrogen-depleted waters with lower chlorophyll concentrations and a picoplankton community dominated by Prochlorococcus and holoplankton predominance in mesozooplankton. Under early autumn conditions with overall nutrient-depleted and stratified waters, the CT area emerges as an ecosystem where the constant tidal mixing and nutrients supply is coupled with an active production also being favored by high residence times and finally shaping a plankton community with unique features in the area.

Selenium (Se) is a vital trace element for many living organisms inclusive of aquatic species. Although the antagonistic action of this element against other pollutants has been previously described for mammals and birds, limited information on the join effects in bivalves is available. To this end, bivalves of the species Scrobicularia plana were exposed to Se and Cd individually and jointly. Digestive glands were analysed to determine dose-dependent effects, the potential influence of Se on Cd bioaccumulationas well as the possible recover of the oxidative stress and metabolic alterations induced by Cd. Selenium co-exposure decreased the accumulation of Cd at low concentrations. Cd exposure significantly altered the metabolome of clams such as aminoacyltRNA biosynthesis, glycerophospholipid and amino acid metabolism, while Se co-exposure ameliorated several altered metabolites such asLysoPC (14:0), LysoPE (20:4), LysoPE (22:6), PE (14:0/18:0), PE (20:3/18:4) andpropionyl-L-carnitine.Additionally, Se seems to be able to regulate the redox status of the digestive gland of clams preventing the induction of oxidativedamage in this organ. This study shows the potential Se antagonism against Cd toxicity in S. plana and the importance to study join effects of pollutants to understand the mechanism underlined the effects.

Pharmaceutical drugs in the aquatic medium may pose significant risk to non-target organisms. In this study, the potential toxicity of a mixture of three compounds commonly detected in marine waters (ibuprofen, ciprofloxacin and flumequine) was assessed, by studying bioaccumulation, oxidative stress and neurotoxicity parameters (catalase CAT, superoxide dismutase SOD, glutathione reductase GR, glutathione S-transferase GST, lipid peroxidation LPO, glutathione peroxidase GPX, metallothionein MT and acetylcholinesterase AChE) in the clam Scrobicularia plana. Temporal evolution of selected endpoints was evaluated throughout an exposure period (1, 7 and 21 days) followed by a depuration phase. The accumulation of all drugs was fast, however clams showed the ability to control the internal content of drugs, keeping their concentration constant throughout the exposure and reducing their content after 7 days of depuration. The induction of biochemical alterations (SOD, CAT, LPO, MT, AChE) was observed in gills and digestive gland probably related to an imbalance in the redox state of clams as a consequence of the exposure to the drug mixture. These alterations were also maintained at the end of the depuration week when the high levels of SOD, CAT, GST and LPO indicated the persistence of oxidative stress and damage to lipids despite the fact that clams were no longer exposed to the mixture.

The redox speciation of iron was determined during the iron fertilization LOHAFEX and for the first time, the chemiluminescence assay of filtered and unfiltered samples was systematically compared. We hypothesize that higher chemiluminescence in unfiltered samples was caused by Fe(II) adsorbed onto biological particles. Dissolved and particulate Fe(II) increased in the mixed layer steadily 6-fold during the first two weeks and decreased back to initial levels by the end of LOHAFEX. Both Fe(II) forms did not show diel cycles downplaying the role of photoreduction. The chemiluminescence of unfiltered samples across the patch boundaries showed strong gradients, correlated significantly to biomass and the photosynthetic efficiency and were higher at night, indicative of a biological control. At 150 m deep, a secondary maximum of dissolved Fe(II) was associated with maxima of nitrite and ammonium despite high oxygen concentrations. We hypothesize that during LOHAFEX, iron redox speciation was mostly regulated by trophic interactions.

Peach [Prunus persica L. Batsch] is one of the major temperate fruit tree species, the commercial materials of which have a low level of genetic variability. Almond [P. dulcis (Mill) DA Webb], a close relative of peach cultivated for its kernels, has a much higher level of diversity. The species are inter-compatible and often produce fertile hybrids, almond being a possible source of new genes for peach that could provide biotic and abiotic stress tolerance traits. In this paper we describe the development of a collection of peach-almond introgression lines (ILs) having a single fragment of almond (cv. Texas) in the peach background (cv. Earlygold). Lines with few introgressions were selected with markers from successive generations from a "Texas"× "Earlygold"F1 hybrid, initially using a set of SSRs and later with the 18 k peach SNP chip, allowing for the final extraction of 67 lines, 39 with almond heterozygous introgressions covering 99% of the genome, and 28 with homozygous introgressions covering 83% of the genome. As a proof of concept, four major genes and four quantitative characters were examined in the selected ILs giving results generally consistent with previous information on the genetics of these characters. This collection is the first of its kind produced in a woody perennial species and promises to be a valuable tool for genetic analyses, including dissection of quantitative traits, positional cloning, epistasis and as prebreeding material to introgress almond genes of interest into the peach commercial gene pool.

Drought is one of the most difficult natural hazards to quantify and is divided into categories (meteorological, agricultural, ecological and hydrological), which makes assessing recent changes and future scenarios extremely difficult. This opinion piece includes a review of the recent scientific literature on the topic and analyses trends in meteorological droughts by using long-term precipitation records and different drought metrics to evaluate the role of global warming processes in trends of agricultural, hydrological and ecological drought severity over the last four decades, during which a sharp increase in atmospheric evaporative demand (AED) has been recorded. Meteorological droughts do not show any substantial changes at the global scale in at least the last 120 years, but an increase in the severity of agricultural and ecological droughts seems to emerge as a consequence of the increase in the severity of AED. Lastly, this study evaluates drought projections from earth system models and focuses on the most important aspects that need to be considered when evaluating drought processes in a changing climate, such as the use of different metrics and the uncertainty of modelling approaches.

This manuscript considers that the composition of Manzanilla and Hojiblanca fats are compositional data (CoDa). Thus, the work applies CoDa analysis (CoDA) to investigate the effect of processing and packaging on the fatty acid profiles of these cultivars. To this aim, the values of the fat components in percentages were successively subjected to exploratory CoDA tools and, later, transformed into ilr (isometric log-ratio) coordinates in the Euclidean space, where they were subjected to the standard multivariate techniques. The results from the first approach (bar plots of geometric means, tetrahedral plots, compositional biplots, and balance dendrograms) showed that the effect of processing was limited while most of the variability among the fatty acid (FA) profiles was due to cultivars. The application of the standard multivariate methods (i.e., Canonical variates, Linear Discriminant Analysis (LDA), ANOVA/MANOVA with bootstrapping and n = 1000, and nested General Linear Model (GLM)) to the ilr coordinates transformed data, following Ward's clustering or descending order of variances criteria, showed similar effects to the exploratory analysis but also showed that Hojiblanca was more sensitive to fat modifications than Manzanilla. On the contrary, associating GLM changes in ilr with fatty acids was not straightforward because of the complex deduction of some coordinates. Therefore, according to the CoDA, table olive fatty acid profiles are scarcely affected by Spanish-style processing compared with the differences between cultivars. This work has demonstrated that CoDA could be successfully applied to study the fatty acid profiles of olive fat and olive oils and may represent a model for the statistical analysis of other fats, with the advantage of applying appropriate statistical techniques and preventing misinterpretations.

The control of the Mediterranean fruit fly (Medfly), Ceratitis capitata, in citrus orchards in Spain is mainly based in three insecticides (spinosad, lambda-cyhalothrin and deltamethrin) and the liberation of sterile males. However, Medfly control is compromised by the development of lambda-cyhalothrin resistance and the detection of spinosad-resistant alleles in field populations. We report here, for the first time, resistance to deltamethrin in populations collected in fields under different management strategies, including MagnetMed™ traps coated with this insecticide and/or spinosad and lambda-cyhalothrin used as bait sprays, and even in populations obtained from non-treated fields. Two deltamethrin-resistant strains (BP-delta and Rfg-delta) were generated from the descendants of some of the field populations that showed lower susceptibility to deltamethrin. Both strains showed low susceptibility to MagnetMed™ traps, moderate susceptibility to Ceratipack traps, and lacked cross-resistance to spinosad and lambda cyhalothrin. Our data suggest that deltamethrin resistance was mediated by P450 enzymes, since bioassays with synergists showed that PBO reverted resistance in a field population and the laboratory strains, whereas the effect of DEF and DEM was minor and no mutations were found in the VGSC gene. The inheritance of resistance for both strains was completely recessive, autosomic and did not fit the mortality expected for a recessive character under a monogenic or digenic model. We also found that deltamethrin resistance presented a fitness cost in terms of males’ weight, males’ and females’ longevity and lifetime fecundity, with a more pronounced effect in the BP-strain than in the Rfg-delta strain. Our results highlight the need to implement insecticide resistance management strategies to prevent control failures.

We report, for the first time, two cases of predation on Red-legged Partridge Alectoris rufa chicks in the process of hatching by the harvester ant Messor barbarus.

Sodium Iodide Thallium doped (NaI(Tl)) scintillation detectors have been applied to the direct searches for dark matter since the 1980s and have produced one of the most challenging results in the field—the observation by the DAMA/LIBRA collaboration of an annual modulation in the detection rate for more than twenty cycles. This result is very difficult to reconcile with negative results derived from other experiments using a large variety of target materials and detection techniques. However, it has been neither confirmed nor refuted in a model independent way up to the present. Such a model independent test of the DAMA/LIBRA result is the goal of the ANAIS-112 experiment, presently in the data taking phase at the Canfranc Underground Laboratory in Spain. ANAIS-112 design and operation leans on the expertise acquired at the University of Zaragoza in direct searches for Dark Matter particles using different targets and techniques and in particular using NaI(Tl) scintillation detectors for about thirty years, which are reviewed in the first section of this manuscript. In addition to presenting the status and more recent results of the ANAIS-112 experiment, open research lines, continuing this effort, will be presented.

This paper studies the relationship between people’s beliefs about the quality of their institutions, as measured by corruption perceptions, and preferences for redistribution in Latin America. Our empirical study is guided by a theoretical model which introduces taxes into Foellmi and Oechslin’s (2007) general equilibrium model of non-collusive corruption. In this model perceived corruption influences people’s preferences for redistribution through two channels. On the one hand it undermines trust in government, which reduces people’s support for redistribution. On the other hand, more corruption decreases own wealth relative to average wealth of below-average-wealth individuals leading to a higher demand for redistribution. Thus, the effect of perceived corruption on redistribution cannot be signed a priori. Our novel empirical findings for Latin America suggest that perceiving corruption in the public sector increases people’s support for redistribution. Although the wealth channel dominates in the data, we also find evidence for the trust channel — from corruption to demand for redistribution via reduced trust.

This study provides a long-term (1891–2014) global assessment of precipitation trends using data from two station-based gridded datasets and climate model outputs evolved through the fifth and sixth phases of the Coupled Model Intercomparison Project (CMIP5 and CMIP6, respectively). Our analysis employs a variety of modeling groups that incorporate low- and high-top level members, with the aim of assessing the possible effects of including a well-resolved stratosphere on the model’s ability to reproduce long-term observed annual precipitation trends. Results demonstrate that only a few regions show statistically significant differences in precipitation trends between observations and models. Nevertheless, this pattern is mostly caused by the strong interannual variability of precipitation in most of the world regions. Thus, statistically significant model-observation differences on trends (1891–2014) are found at the zonal mean scale. The different model groups clearly fail to reproduce the spatial patterns of annual precipitation trends and the regions where stronger increases or decreases are recorded. This study also stresses that there are no significant differences between low- and high-top models in capturing observed precipitation trends, indicating that having a well-resolved stratosphere has a low impact on the accuracy of precipitation projections.

The color of virgin olive oils, ranging from intense green to brown-yellow, is one of the main selection factors for consumers and a quality criterion in specific legislations. Such coloration is due to their chlorophyll content and depends on the composition of the olive fruit. Through analytical chemistry (HPLC-hrMSn), biochemistry (enzymatic activity), and molecular biology (qRT-PCR) approaches, we have analyzed the origin of the differences in the chlorophyll content among several varieties of olive fruit throughout their ripening process. The higher chlorophyll biosynthetic capacity in olive fruits is due to the enzyme protochlorophyllide reductase, whereas chlorophyll degradation is accomplished through the stay-green and pheophytinase pathways. For the first time, the implication of chlorophyll dephytylase during the turnover of chlorophylls in fruit is shown to be responsible for the exclusive accumulation of dephytylated chlorophyll in Arbequina fruit. The multiomics results excluded the in vivo participation of chlorophyllase in chlorophyll degradation in olive fruits.

Soft fruits are appreciated for their taste qualities and for being a source of health-promoting compounds. However, their postharvest is affected by their high respiratory rates and susceptibility to fungal decay. Our aim here is to provide a perspective on the application of short-term high-CO2 treatments at a low temperature to maintain the postharvest quality of soft fruits. This work also suggests using a multi-omics approach to better understand the role of the cell wall and phenolic compounds in maintaining quality. Finally, the contribution of high-throughput transcriptomic technologies to understand the mechanisms modulated by the short-term gaseous treatments is also highlighted.

[Background]: Chronic lymphocytic leukemia (CLL) is the most frequent, and still incurable, form of leukemia in the Western World. It is widely accepted that cancer results from an evolutionary process shaped by the acquisition of driver mutations which confer selective growth advantage to cells that harbor them. Clear examples are missense mutations in classic RAS genes (KRAS, HRAS and NRAS) that underlie the development of approximately 13% of human cancers. Although autonomous B cell antigen receptor (BCR) signaling is involved and mutations in many tumor suppressor genes and oncogenes have been identified, an oncogenic driver gene has not still been identified for CLL. [Methods]: Conditional knock-in mice were generated to overexpress wild type RRAS2 and prove its driver role. RT-qPCR analysis of a human CLL sample cohort was carried out to measure RRAS2 transcriptional expression. Sanger DNA sequencing was used to identify a SNP in the 3’UTR region of RRAS2 in human CLL samples. RNAseq of murine CLL was carried out to identify activated pathways, molecular mechanisms and to pinpoint somatic mutations accompanying RRAS2 overexpression. Flow cytometry was used for phenotypic characterization and shRNA techniques to knockdown RRAS2 expression in human CLL. [Results]: RRAS2 mRNA is found overexpressed in its wild type form in 82% of the human CLL samples analyzed (n = 178, mean and median = 5-fold) as well as in the explored metadata. A single nucleotide polymorphism (rs8570) in the 3’UTR of the RRAS2 mRNA has been identified in CLL patients, linking higher expression of RRAS2 with more aggressive disease. Deliberate overexpression of wild type RRAS2 in mice, but not an oncogenic Q72L mutation in the coding sequence, provokes the development of CLL. Overexpression of wild type RRAS2 in mice is accompanied by a strong convergent selection of somatic mutations in genes that have been identified in human CLL. R-RAS2 protein is physically bound to the BCR and mediates

The partially overlapped ferroelastic/martensitic and para-ferromagnetic phase transitions of a Ni50.53Mn33.65In15.82 metamagnetic shape memory alloy have been studied from calorimetric, magnetic and acoustic emission measurement. We have taken advantage of the existence of thermal hysteresis of the first order ferroelastic/martensitic phase transition (~2.5 K) to discriminate the latent heat contribution Delta=H 7.21(15)kJ/kg and the specific heat contribution Delta H_c =216(1)J/kg to the total excess enthalpy of the phase transition. The specific heat was found to follow a step-like behavior at this phase transition. The intermittent dynamics of the ferroelastic/martensitic transition has been characterized as a series of avalanches detected both from acoustic emission and calorimetric measurements. The energy distribution of these avalanche events was found to follow a power law with a characteristic energy exponent 2 which is in agreement with the expected value for a system undergoing a symmetry change from cubic to monoclinic. Finally, the critical behavior of the para-ferromagnetic austenite phase transition that takes place at ~311 K has been studied from the behavior of the specific heat. A critical exponent 0.09 has been obtained, which has been shown to be in agreement with previous values reported for Ni-Mn-Ga alloys but different from the critical divergence reported for pure Ni.

Microplastics (<5 mm; MPs) are globally recognized as an issue of emerging concern in the marine environment. In this study, MPs were determined for the first time in sediments from Rias Baixas and Mino river shelf, to show the baseline contamination levels. The Rias is well known for its intense fishing and shellfish harvesting activities. The sampling stations were selected due to their different exposure to anthropogenic activities and/or pressures. Sediment samples were collected using a box corer dredge and analyzed with density separation and mu F-TIR analysis to determine polymer type. The results showed MPs pollution in all stations. The mean concentration was 70.2 +/- 74.2 particles/kg DW. The main MPs shape was fibers (88.8%) followed by fragments (5.80%). Regarding the polymers, the most abundant were PP (43%) and HDPE (29%). Once again, MPs are ubiquitous all along Rias and Mino river mouth as previously shown in different works.

Microplastics are gaining worldwide attention due to their omnipresence. The marine environment is one of the most affected systems; especially the sediment compartment. Microplastic separation from the sediment matrix is the first step to evaluate its abundance and availability. Nevertheless, a lack of consistency in extraction protocols is a fact. This paper describes the optimization of the microplastic extraction procedure from marine sediments. The Plackett-Burman saturated factorial design was used to identify the significant factors and to select optimum working conditions. With this purpose, the following variables were studied: the number of extractions; the amount of sediment; the settling time; the density separation solution volume; the agitation tim and the suitability of using wet or freeze-dried sediment. The Plackett-Burman design has revealed that the mos statistically significant variables were sediment mass and agitation time. The optimized method was applied for two marine sediments collected in the Mar Menor Lagoon.

The seasonal bioaccumulation of trace metals, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorinated pesticides (OCPs) in sea snail (Hexaplex trunculus) and sea cucumber (Holothuria polii) from Mar Menor lagoon were characterised. The highest concentrations of p,p'-DDE were detected in the central and south part of Mar Menor lagoon. However, the highest concentrations of metals in sea snail and holothurians were detected in the influence area of El Beal wadi. Biomagnification factors (BMF) in sea snail from cockle (sea snail-cockle concentration ratio) were higher than 5 for metal and organochlorinated compounds. However, similar concentrations were observed in both species for PAHs due to gastropods capability of metabolising these pollutants. Consequently, sea snail is proposed as a sentinel for trace metals, PCBs and OCPs in the coastal lagoons, not only due to its bioaccumulation and biomagnification capacity but also the easy sampling and amply distribution in many coastal areas.

Despite the fact that many studies have examined the effectiveness of different gaseous postharvest treatments applied at low temperature to maintain table grape quality, the use of ethanol vapor has hardly been investigated. Thus, this work has studied the effectiveness of ethanol vapor-generating sachets in the maintenance of It 681–30 table grape quality, a new cultivar, during storage at low temperature and after the shelf-life period at 20 °C. To this end, various quality assessments have been carried out and the effect of the ethanol treatment on the expression of different genes (phenylpropanoids, transcription factors, PRs, and aquaporins) was determined. The results indicated that the application of ethanol vapor reduced the total decay incidence, weight loss, and the rachis browning index in It 681–30 grapes stored at 0 °C and after the shelf-life period at 20 °C, as compared to non-treated samples. Moreover, the modulation of STS7 and the different PR genes analyzed seems to play a part in the molecular mechanisms activated to cope with fungal attacks during the postharvest of It 681–30 grapes, and particularly during the shelf-life period at 20 °C. Furthermore, the expression of aquaporin transcripts was activated in samples showing higher weight loss. Although further work is needed to elucidate the role of ethanol in table grape quality, the results obtained in this work provide new insight into the transcriptional regulation triggered by ethanol treatment.

The dynamics of blue and green water partitioning under vegetation and climate change, as well as their different interactions during wet and dry periods, are poorly understood in the literature. We analyzed the impact of vegetation changes on blue water generation in a central Spanish Pyrenees basin undergoing intense afforestation. We found that vegetation change is a key driver of large decreases in blue water availability. The effect of vegetation increase is amplified during dry years, and mainly during the dry season, with streamflow reductions of more than 50%. This pattern can be attributed primarily to increased plant water consumption. Our findings highlight the importance of vegetation changes in reinforcing the decrease in water resource availability. With aridity expected to rise in southern Europe over the next few decades, interactions between climate and land management practices appear to be amplifying future hydrological drought risk in the region.

Dehydrins, a family of hydrophilic and intrinsically disordered proteins, are a subgroup of late embryogenesis abundant proteins that perform different protective roles in plants. Although the transition from a disordered to an ordered state has been associated with dehydrin function or interactions with specific partner molecules, the question of how the primary and secondary dehydrin protein structure is related to specific functions or target molecule preferences remains unresolved. This work addresses the in silico sequencing analysis and in vitro functional characterization of two dehydrin isoforms, VviDHN2 and VviDHN4, from Vitis vinifera. Conformational changes suggest potential interactions with a broad range of molecules and could point to more than one function. The in silico analysis showed differences in conserved segments, specific amino acid binding sequences, heterogeneity of structural properties and predicted sites accessible for various post-translational modifications between the sequence of both dehydrins. Moreover, in vitro functional analysis revealed that although they both showed slight antifungal activity, only VviDHN4 acts as a molecular shield that protects proteins from freezing and dehydration. VviDHN4 also demonstrated high potential as a chaperone and reactive oxygen species scavenger, in addition to presenting antifreeze activity, all of which confirms its multifunctional nature. Our findings highlight the significant role of Y-segments and the differential and specific amino acid composition of less conserved segments that are rich in polar/charged residues between S- and K-segments, coupled with post-translational modifications, in modulating and switching dehydrin biological function.

We analyzed the impacts of drought severity on a variety of sectors in a topographically complex basin (the upper Aragón basin 2181 km2) in the Central Spanish Pyrenees. Using diverse data sources including meteorological and hydrological observations, remote sensing and tree rings, we analyze the possible hydrological implications of drought occurrence and severity on water availability in various sectors, including downstream impacts on irrigation water supply for crop production. Results suggest varying responses in forest activity, secondary growth, plant phenology, and crop yield to drought impacts. Specifically, meteorological droughts have distinct impacts downstream, mainly due to water partitioning between streamflow and irrigation channels that transport water to crop producing areas. This implies that drought severity can extend beyond the physical boundaries of the basin, with impacts on crop productivity. This complex response to drought impacts makes it difficult to develop objective basin-scale operational definitions for monitoring drought severity. Moreover, given the high spatial variability in responses to drought across sectors, it is difficult to establish reliable drought thresholds from indices that are relevant across all socio-economic sectors. The anthropogenic impacts (e.g. water regulation projects, ecosystem services, land cover and land use changes) pose further challenges to assessing the response of different systems to drought severity. This study stresses the need to consider the seasonality of drought impacts and appropriate drought time scales to adequately assess and understand their complexity.

We analysed long‐term variability and trends in meteorological droughts across Western Europe using the Standardized Precipitation Index (SPI). Precipitation data from 199 stations spanning the period 1851–2018 were employed, following homogenisation, to derive SPI‐3 and SPI‐12 series for each station, together with indices on drought duration and severity. Results reveal a general absence of statistically significant long‐term trends in the study domain, with the exception of significant trends at some stations, generally covering short periods. The largest decreasing trends in SPI‐3 (i.e., increasing drought conditions) were found for summer in the British and Irish Isles. In general, drought episodes experienced in the last two or three decades have precedents during the last 170·years, emphasizing the importance of long records for assessing change. The main characteristic of drought variability in Western Europe is its strong spatial diversity, with regions exhibiting a homogeneous temporal evolution. Notably, the temporal variability of drought in Western Europe is more dominant than long‐term trends. This suggests that long‐term drought trends cannot be confirmed in Western Europe using precipitation records alone. This study provides a long‐term regional assessment of drought variability in Western Europe, which can contribute to better understanding of regional climate change during the past two centuries.

This study presents a climatology and trend analysis of reference crop evapotranspiration (ETo) over continental Spain and the Balearic Islands. Geographic features of the study region play a substantial role in the climatology of ETo. The highest values (in excess of 1,200 mm y−1) are found at lower elevations in the south, while the lowest values (less than 900 mm y−1) are found in the highest elevations in the north. A deep analysis reveals: (a) a low interannual variability; (b) summer accumulates more than 50% of annual values; and (c) the radiative component contribution is higher than 50%. A positive long‐term trend (1961–2014) has been detected for most of the study area, but showing contrasting situations when shorter periods (20–30 years) are analysed. A short initial period of negative trend was followed by a longer period of a positive trend. We argue that global dimming/brightening played a role in this process, as these two contrasting periods are clearly guided by the radiative component. A seasonal analysis of the trends reveals that spring and summer are the seasons showing the long‐term positive trends. Interestingly, a monthly analysis shows that spring trends are guided by March and April and summer trends are mostly guided by June, which is the month showing the highest relative changes. This could have important consequences for agriculture and natural ecosystems since this month represents the start of the summer (dry) period.

Anthropogenic activities, such as the translocation or introduction of animals, may cause a parallel movement of exotic parasites harboured by displaced animals. Although introduction and/or relocation of animals for hunting purposes is an increasingly common management technique, the effects of gamebird release as a major vehicle for the introduction of parasites into new geographic regions have rarely been reported. We examined the prevalence and distribution of avian malaria parasites infecting resident avian hosts (red-legged partridge Alectoris rufa) at a local scale, with a particular emphasis on the effects of releasing farm-reared birds for hunting on the spatial and temporal structure of the parasite community. We collected blood samples from adult partridges from two game estates with partridge releases and two sites without releases over two periods (spring and autumn). We tested the probability of infection and differences in the parasite community in relation to the management model (releases vs. non releases) and sampling period, comparing autumn (when farm-reared birds are released) and spring (after hunting season, when mostly wild birds can be found in the population). We found a high prevalence (54%) of Plasmodium spp., and substantial differences in the spatial and temporal distribution of parasite lineages among the populations studied. Some parasite lineages occurred at high frequencies in game estates without introduction of farm-reared partridges, while other lineages were more abundant in game estates with releases than in those without releases. Overall, the prevalence of avian malaria was similar between spring and autumn at non-release sites, whereas in sites with releases, it was higher in autumn than in spring—probably due to artificial restocking with infected farm-reared birds at the onset of the hunting season. In short, humans may be an important agent driving the alteration of the spatial structure of local parasite fauna via the in

Background: In the last few years there has been emerging interest in substituting added sugars from juices with other sweeteners to make them healthier. But their long-term effects have been poorly evaluated. The aim of this study is to evaluate the effect of the addition of stevia, sucralose and sucrose (control) to maqui-citrus beverages on antioxidant and inflammatory status. Methods: a 3-arm parallel, randomized and triple blind clinical trial was performed in overweight subjects (n = 138), who consumed the test beverage (330 mL day-1) for 60 days. The following markers were determined: antioxidant status (ORAC, homocysteine, and oxidized LDL), safety parameters (ALP, AST, ALT, and total bilirubin), lipid profile (total cholesterol, LDL-cholesterol, HDL-cholesterol, and triglycerides) and inflammatory biomarkers (IL-6, TNF-a, and IL-10). Results: The homocysteine levels significantly increased after consumption of sucralose (27%, p = 0.001) and sucrose (40%, p = 0.006). A significant increase in the IL-10 concentration after consumption of the stevia sweetened beverage, and in ORAC values (21%) in subjects with lower basal antioxidant status were observed. The HDL and total cholesterol levels significantly increased after consumption of sucralose (p = 0.039) and sucrose (p = 0.001), respectively. No changes in triglycerides, LDL or oxidized LDL were observed. Conclusions: Oxidative stress and an inflammatory response were observed after consumption of these sweetened beverages, with the exception of stevia, which produced an anti-inflammatory response. The possible antioxidative effects of this polyphenol-rich beverage may only benefit those individuals with poorer antioxidant status. Many randomized controlled trials at normal levels of consumption using commonly consumed sweeteners are necessary to clarify their roles in health.

BACKGROUND.The control of the Mediterranean fruit fly Ceratitis capitata (Wiedemann) in Spanish field populations mainly relies on the insecticides lambda-cyhalothrin and spinosad as bait sprays. However, their sustainable used is compromised by the development of lambda-cyhalothrin resistance and the detection of spinosad resistant alleles. In addition, the use of lure-and-kill traps covered with deltamethrin has increased in the last years. It is thus urgent to predict the impact that the combination of both pyrethroids will have in the evolution of lambda-cyhalothrin resistance and how they could be combined with spinosad so as to establish proper resistance management programs., RESULTS.Toxicity bioassays were performed to analyze the current levels of lambda-cyhalothrin resistance in field populations, proving that it has remained stable in the last decade. An evolutionary model was established to explore the weight of selected parameters in the evolution of lambda-cyhalothrin resistance in C. capitata and to forecast resistance development under different resistance management scenarios. Our results highlight the importance of fitness cost and inheritance to fit the experimental results. The analyses predicted that the rotation of lambda-cyhalothrin and spinosad, when deltamethrin traps are also deployed in the field, will slow down the evolution of resistance, especially when cross-resistance between both pyrethroids is considered., CONCLUSION.Lambda-cyhalothrin resistance has not increased in the last decade, probably due to the alternation of this insecticide with spinosad. Our modelling results indicate that the best option to avoid an increase in lambda-cyhalothrin resistant alleles, considering that deltamethrin use is growing, would be to continue combining their use with spinosad.

This study compares the Medieval (ca. 400–1500 CE; Common Era) dynamics of forests from low-mountain (Montcortès; ca. 1000 m a.s.l.) and high-mountain (Sant Maurici; 1900 m a.s.l.) areas of the Iberian Pyrenees, both of which experienced similar climatic forcing but different anthropogenic pressures. The main aim is to identify forest changes over time and associate them with the corresponding climatic and anthropogenic drivers (or synergies among them) to test how different forests at different elevations respond to external forcings. This can be useful to evaluate the hypothesis of general Pyrenean deforestation during the Middle Ages leading to present-day landscapes and to improve the background for forest conservation. The study uses the palynological analysis of lake sediments, historical documents and paleoecological reconstructions based on pollen-independent proxies. The two sites studied showed different forest trajectories. The Montcortès area was subjected to intense human pressure during regional deforestation up to a maximum of ca. 1000 CE. Further forest recovery took place until the end of the Middle Ages due to a change in forest management, including the abandonment of slash-and-burn practices. Climatic shifts indirectly influenced forest trends by regulating human migrations and the resulting shifts in the type and intensity of forest exploitation. The highland Sant Maurici forests exhibited a remarkably long-standing constancy and an exceptional resilience to climatic shifts, which were unable to affect forest extension and composition, and to local human pressure, from which they rapidly recovered. The Montcortès and Sant Maurici records did not follow the rule of an irreversible forest clearing during the Middle Ages leading to present-day landscapes. The present Montcortès landscape was shaped after a Medieval forest recovery, new Modern-Age deforestation and further forest recovery during the last centuries. The Sant Maurici forests remained

Most available results on optimal decisions under partial information are derived under “separation”. But this principle does not always hold. We derive a non-standard first order condition of optimality from first principles when signal extraction and optimal policy must be jointly determined. This allows us to solve a model of optimal fiscal policy where separation does not apply. Tax smoothing prevails in normal times, but taxes respond strongly in recessions. This non-linearity arises because signal extraction interacts differently with optimal policy depending on the value of the observed signals. Existing results based on the “separation principle” follow as special cases.

We consider a Gellnerian model to study the transformation of a two-region state into a nation state. Industrialization requires the elites to finance schooling. The implementation of statewide education generates a common national identity, which enables cross-regional production, while regional education does not. We show that statewide education is chosen when cross-regional production opportunities and productivity are high, especially when the same elite holds power at both geographical levels. By contrast, a dominant regional elite might prefer regional schooling, even at the loss of large cross-regional production opportunities if it is statewide dominated. The model is consistent with evidence for five European countries in 1860–1920.