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International audience; Ichang lemon is a citrus fruit whose rind gives off a delicious and much appreciated fragrance and flavor. The volatile components of the fruit peel of Ichang lemon were investigated by GC-MS and GC-O (AEDA method). Simultaneously, its genetic origin was identified by using diagnostic SNP markers specific to ancestral species and multiallelic SSR and InDel markers. Ichang lemon combines three ancestral genomes (Citrus maxima, Citrus ichangensis, and Citrus reticulata) and may be a pummelo x Yuzu hybrid. Although the major compounds of the Ichang lemon aromatic profile were present in Citrus junos, a few pummelo-specific compounds were also detected, such as indole and nootkatone, in agreement with its maternal lineage. 3-Methyl-3-sulfanylbutyl acetate, reported to occur in passion fruit and brewed coffee, was identified by GC-MS, GC-QTOF-MS, and GC-FTIR for the first time in citrus. This odor-active compound has a sulfurous, tropical fruity, green note.

Citrus juices can be cold-concentrated by crossflow microfiltration (CMF) in order to obtain functional foods enriched in carotenoids, flavonoids and pectins. The work aimed to characterize the organoleptic quality of this type of micronutrient-dense foods through their aroma profile and sensory analysis. Two citrus concentrates with and without a diafiltration step were compared.Both citrus products were very different, linked to aroma compound, sugar and organic acid contents. Due to its sugar/acidity balance and its better aromatic profile responsible for the citrus-floral flavour, the concentrate without diafiltration was preferred by the sensory panel. Thanks to a simple transfer model, we showed that retention of volatiles clearly varied from one aroma compound to another. The terpene hydrocarbons were the most retained by the membrane during CMF, probably because they were strongly associated with insoluble solids by adsorption.Even though the process modified their organoleptic profiles, both citrus-based products were well rated and can be consumed directly as pleasant functional drinks. © 2022 Society of Chemical Industry.

Post-harvest processing (PHP) modifies the quality of the coffee and increases the value of coffee production. The choice of PHP to apply depends primarily on the available infrastructure, local climatic conditions and the desired end-value. The objective of this study was to evaluate the influence of PHP on the physico-chemical characteristics of green and roasted coffee beans and their sensory attributes. Coffee cherries from IAPAR 59 cultivar were processed as natural coffee (CN), semi-dry coffee (CD), de-pulped coffee (CP) and floating coffee (CF). Total and reducing sugars, phenolic compounds, chlorogenic acids, lipids, proteins, caffeine and water content were determined in coffee beans collected during (10thday) and at the end of each processing. The roasted beans and their sensory attributes were also analyzed. The greatest changes at the ending point of the processes were found in total and reducing sugars, phenolic compounds, chlorogenic acids and lipids contents. The PHP presented different weight loss to achieve the same visual brown color and luminosity. Beverage sensory attributes were influenced by the PHP: CN and CP coffees presented similar intensities of coffee aroma, but higher intensity of green grassy aroma, and taste were found in CF and CN coffees. Aroma and taste precursors were modified during the PHP and these were associated to husk removal of the coffee beans, suggesting an activation of the germination metabolism during the PHP. This study allowed the characterization of the effects of the different post-harvest processing on the roasted coffee beans and provides the foundations to monitor their efficiencies in the future. Made available in DSpace on 2022-01-25T14:00:42Z (GMT). No. of bitstreams: 1 Flavor-precursors-and-sensory-attributes.pdf: 573175 bytes, checksum: 368650d928db2098f3cb1c6ea8173d83 (MD5) Previous issue date: 2020

Cocoa pod-opening delay and bean fermentation promote the organoleptic quality of chocolate. The present research investigated the changes in the volatile fingerprint of cocoa harvested at a traditional plantation. Cocoa beans extracted from 2-days pod-opening delay were simultaneously fermented for 5 days using container and then sun-dried to 7-8% moisture content at five different locations: Akoupé, San Pedro, Soubré, Djekanou and Daloa. The aromatic analysis were done on cocoa using the HS-SPME-GC/MS technique. Professional panelists evaluated the sensory perceptions of the chocolate. The results shows that cocoa fermented in both Daloa and Soubré regions were differentiated by 2,3-butanediol while those processed in other regions presented highest acetoin content. However, fermented cocoa from Soubré region exhibited most amount of 2,3-butanediol, diacetate A whereas 2,3,5,6-tetramethylpyrazine differentiated those from Daloa region. Sensory properties of chocolate were not linked to the aromatic compound precursors profile of beans. The fermentation performed in San Pédro region promote both the generation of more desirable aromatic compounds of cocoa and sensory attributes of the finished chocolate. The fermentation location generates a greater differentiation of the volatile fingerprint of cocoa and the sensory perceptions of the finished chocolate.

The African citrus triozid, Trioza erytreae Del Guercio (Hemiptera: Triozidae) is one of the primary vectors of the bacterium Candidatus Liberibacter spp. which causes citrus greening, a disease of global economic importance in citrus production. Despite its economic importance, little is known about its chemical ecology. Here, we used behavioral assays and chemical analysis to study the chemical basis of interaction between T. erytreae and one of its preferred host plants, Citrus jambhiri. In dual choice Y-tube olfactometer assays, lemon leaf odors attracted females but not males compared to plain air or solvent controls. However, in a petri dish arena assay, both sexes were arrested by lemon leaf odors. Coupled gas chromatography-mass spectrometry (GC/MS) analysis revealed quantitative differences in the odors of flushing and mature leaves, dominated by terpenes. Twenty-six terpenes were identified and quantified. In Petri dish arena assays, synthetic blends of the most abundant terpenes mimicking lemon flushing leaf odors elicited varying behavioral responses from both sexes of T. erytreae. A nine-component blend and a blend of the three most abundant terpenes; limonene, sabinene and β-ocimene arrested both sexes of T. erytreae. In contrast, a six-component blend lacking in these three components elicited an avoidance response in both sexes. Furthermore, both sexes of T. erytreae preferred the three-component synthetic blend to lemon crude volatile extract. These results suggest that lemon terpenes might be used in the management of T. erytreae.

Theobroma cacao is the only source that allows the production of chocolate. It is of major economic importance for producing countries such as Ecuador, which is the third-largest cocoa producer in the world. Cocoa is classified into two groups: bulk cocoa and aromatic fine flavour cocoa. In contrast to bulk cocoa, fine flavour cocoa is characterised by fruity and floral notes. One of the characteristics of Nacional cocoa, the emblematic cocoa of Ecuador, is its aromatic ARRIBA flavour. This aroma is mainly composed of floral notes whose genetic and biochemical origin is not well-known. This research objective is to study the genetic and biochemical determinism of the floral aroma of modern Nacional cocoa variety from Ecuador. Genome-Wide Association Study (GWAS) was conducted on a population of 152 genotypes of cocoa trees belonging to the population variety of modern Nacional. Genome-Wide Association Study was conducted by combining SSR and SNP genotyping, assaying biochemical compounds (in roasted and unroasted beans), and sensory evaluations from various tastings. This analysis highlighted different areas of association for all types of traits. In a second step, a search for candidate genes in these association zones was undertaken, which made it possible to find genes potentially involved in the biosynthesis pathway of the biochemical compound identified in associations. Our results show that two biosynthesis pathways seem to be mainly related to the floral note of Nacional cocoa: the monoterpene biosynthesis pathway and the L-phenylalanine degradation pathway. As already suggested, the genetic background would therefore appear as largely explaining the floral note of cocoa.

Most of volatile flavour compounds occurred during the cocoa postharvest operations including pod storage and fermentation whom effects on these metabolites were aimed by the present study. Three different spontaneous fermentation treatments were performed on the farm, and the main isolated yeasts were identified using PCR-DGGE technique. Fermented cocoa beans were solar-dried, and their flavour compounds were identified using solid phase microextraction technique–gas chromatography–mass spectrometry. The main results showed that Pichia kudriavzevii was common yeast involved in the cocoa fermentation. Thirty flavour precursor compounds grouped into six chemical families including alcohols and pyrazines were identified in raw cocoa samples. ACP showed that short durations of pod storage and fermentation promote the formation of flavour precursors of cocoa. No spontaneous cocoa fermentation treatment influenced the occurrence of aroma compounds. The generation of flavour compounds depends only on the pod storage and the fermentative microorganisms of cocoa.

Vanilla flavor is derived from the cured fruits of two orchid species Vanilla planifolia and V. × tahitensis. About twenty other Vanilla species are also credited with scented fruits, but very little is known about their aromatic features. We investigated the aromatic compound content of the ripe fruits of 30 vanilla accessions obtained from eight species and three hybrids. Glucovanillin plus six phenolic and one anisic aglycones, extracted after enzymatic hydrolysis, were quantified by High-Performance Liquid Chromatography and Ultra-Violet detection. The analysis of the aromatic contents and the principal components showed clear-cut differences in the aromatic content of the species and varieties studied. The total content of aromatic compounds separated the three species most distantly related to V. planifolia, namely V. lindmaniana, V. crenulata and V. imperialis, from the other species notably by the absence of vanillin potential and a very low total aromatic potential (

Dark chocolate samples were previously classified into four sensory categories. The classification was modelled based on volatile compounds analyzed by direct introduction mass spectrometry of the chocolates&rsquo, headspace. The purpose of the study was to identify the most discriminant odor-active compounds that should characterize the four sensory categories. To address the problem, a gas chromatography-olfactometry (GC-O) study was conducted by 12 assessors using a comparative detection frequency analysis (cDFA) approach on 12 exemplary samples. A nasal impact frequency (NIF) difference threshold combined with a statistical approach (Khi&sup2, test on k proportions) revealed 38 discriminative key odorants able to differentiate the samples and to characterize the sensory categories. A heatmap emphasized the 19 most discriminant key odorants, among which heterocyclic molecules (furanones, pyranones, lactones, one pyrrole, and one pyrazine) played a prominent role with secondary alcohols, acids, and esters. The initial sensory classes were retrieved using the discriminant key volatiles in a correspondence analysis (CA) and a hierarchical cluster analysis (HCA). Among the 38 discriminant key odorants, although previously identified in cocoa products, 21 were formally described for the first time as key aroma compounds of dark chocolate. Moreover, 13 key odorants were described for the first time in a cocoa product.

The fine flavor cocoa (FFC) market offers cocoa farmers better monetary and nonmonetary benefits than the bulk market. In this work, during cocoa fermentation, flavor formation was studied at different fermentation times based on sensory profiles, volatile compound contents and untargeted metabolomics. It was observed that chocolate quality is influenced by fermentation time. Thus, at 72 h, the sensory profiles showed no outstanding attributes, while at 96 h, the global quality presented a stronger influence of fine attributes, such as fruitiness, florality, spices and nuttiness. Finally, at 120/144 h, these FFC features diminished. Metabolomic fingerprint of cocoa beans (related to peptides, sugars, amino acids, and phenolic compounds) and the volatile fingerprint of chocolate showed a change according to the fermentation time. This allowed the proposal of 96 h as the optimal fermentation time to produce FFC beans. Additionally, 20 volatiles and 48 discriminating metabolites were defined as potential quality biomarkers.

The variegated coffee bug Antestiopsis thunbergii Gmelin is a key pest of Coffea arabica in East Africa. Although the bug feeds on various parts of the coffee plant, it has a strong preference for mature green berries which are essential for the bug to complete its life cycle, reproduce and enhance its longevity. To locate mature green coffee berries, we hypothesized that second instar nymphs, which are the most mobile and active feeding immature stage of the bug, must rely on key volatile compounds emitted by the host for recognition. We tested this hypothesis using behavioral and electrophysiological assays and chemical analysis. In olfactometer assays, the second instar nymphs were strongly attracted to volatiles emitted from mature green berries but avoided those from ripe coffee berries of C. arabica. Coupled gas chromatography–electroantennographic detection (GC/EAD) isolated five antenally active components from mature green berries volatiles, four of which were identified by coupled GC–mass spectrometry as toluene, anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin. In concentration assays, in which second instar nymphs did not respond to toluene, they were strongly attracted to anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin, and a blend from the three compounds at concentrations lower or equivalent to the natural volatile extract. Our results suggest that the blend from these three compounds allows host recognition in second instar nymphs of the variegated coffee bug and is a candidate kairomone for monitoring the pest in coffee plantations.

International audience; Low pungency, high aromatic potential and red color, give to Piper borbonense its originality when compared to Piper nigrum. Effects of blanching, sweating and drying on these characteristics were assessed. The three operations had no impact on the concentration of piperine and essential oil but affected the composition of essential oil slightly and considerably affected the color of the pepper. The ''wet process " , including blanching, sweating and drying, had the largest impact on the composition of aroma, increasing para-cymene content by 89% and reducing safrole content by 33% in dried pepper compared to fresh. Blanching increased the drying rate thus reducing drying time. Drying had a major impact on color, which changed from red to brown. The biggest differences observed led to reductions of 2.2, 7.9 and 8.4 units in L ⁄ ,a ⁄ and b ⁄ values, when chromatic values measured in fresh pepper were compared to those of dried pepper.

Combined steps, such as draining and acidification, as well as ripening downtime still represent bottlenecks during cheese making. Indeed, they negatively affect the control of process efficiency, the cheese weight range, the quality of by-products and the balance between supply and demand. In this work, an innovative process was proposed and validated on a semi-industrial scale in order to manufacture cheese products without ripening, albeit on a large range of texture and taste mimicking fresh to pressed type cheeses. On the one hand, draining and acidification steps were uncoupled by using membrane filtration and centrifugation to fractionate milk constituents, and then by reconstructing a controlled texture matrix with regard to protein / fat ratio, denaturation rate of whey proteins, mineralization and amount of available lactose.Different aromatic matrices were produced in parallel by growing Yarrovia lipolytica, Propionibacterium freudenreichii, a mixture of Lactococcus lactis / cremoris / diacetylactis, or Hafnia alvéi in controlled environmental conditions. In less than four days, aromatic sulfur, ketone, acidic and aldehyde molecules were produced at concentration similar to that of a ripened curd. Finally, the texture and aromatic matrices were assembled in a ratio 90:10, and then textured through pH and temperature adjustment, NaCl, rennet or texturing agent addition. Results showed it was possible to obtain products with firmness varying from that of a spread cheese to that of a semi hard type cheese, and with aroma mimicking Saint Paulin as well as Cheddar depending on the quantities and proportions of added aromatic molecules.This eco-efficient and patented process makes it possible to control each step independently, to reduce significantly the inputs, to obtain standardized by-products and consequently to limit production costs. It is possible with the same equipment to produce from day to day and on demand any types of flavored and textured cheeses.

Two series of compounds showing mass signals at m/z 605 and 893 (negative ionization mode) have been detected in fermented cocoa beans. This study objective is to identify these mass signals and characterize their structure in fermented cocoa samples. Our hypothesis is that these signals may correspond to ethyl-bridged flavan-3-ols resulting from flavan-3-ol condensation with acetaldehyde which is a microbial metabolite. Mass spectrometry was used to compare the retention times and mass fragmentation patterns between a model solution using epicatechin and procyanidin dimer B2, the major flavan-3-ols of cocoa, as precursors and extracts of fermented cocoa. Their identification was confirmed: four isomers of ethyl-linked epicatechin as well as several isomers of epicatechin-ethyl-procyanidin B2, in which B2 was mostly linked through its upper unit, were characterized in cocoa. This study demonstrates the presence of flavan-3-ol acetaldehyde condensation products in fermented cocoa beans and provides the first report of epicatechin-ethyl-procyanidin B2.

Baobab (genus Adansonia, family Malvaceae) comprises nine species distributed worldwide of which seven are present in Madagascar. Six are endemic to Grande Ile and are distributed in two sections: Brevitubae and Longitubae. The aim of this study was to distinguish the qualitative and quantitative characters of floral emissions by different species of Malagasy baobabs and to propose hypotheses on their modes of pollination. Floral fragrance was analysed using gas chromatography and mass spectrometry. The study revealed two sections which were distinguishable by the quality and quantity of the compounds they emitted. Species belonging to the Longitubae section all emit a large quantity of compounds with aromatic structures, dominated by 2-phenylacetonitrile, whereas the fragrances of the two species of Brevitubae are rich in compounds with aliphatic structures, particularly 8-heptadecene. The majority of chemicals identified in the Longitubae section are common components of a wide array of scented flowers pollinated by moths such as Sphingidae. However, in the Brevitubae section, no clear relation was found between chemical composition of the fragrance and a particular type of pollinator. The presence of common components in the fragrances in every Longitubae species made it possible to analyse the action of common pollinators and to explain the cases of genetic introgression between the species in this section.

International audience; Beverage quality is a complex attribute of coffee ( Coffea arabica L.). Genotype (G), environment (E), management (M), postharvest processing, and roasting are all involved. However, little is known about how G × M interactions influence beverage quality. We investigated how yield and coffee leaf rust (CLR) disease (caused by Hemileia vastatrix Berk. et Br.) management affect cup quality and plant performance, in two coffee cultivars. Sensory and chemical analyses revealed that 10 of 70 attributes and 18 of 154 chemical volatile compounds were significantly affected by G and M. Remarkably, acetaminophen was found for the first time in roasted coffee and in higher concentrations under more stressful conditions. A principal component analysis described 87% of the variation in quality and plant overall performance. This study is a first step in understanding the complexity of the physiological, metabolic, and molecular changes in coffee production, which will be useful for the improvement of coffee cultivars.

Given the growing interest in high quality hibiscus teas and the scarcity of information about their sensory profile, lexicons were developed in French, Portuguese, and English. Twenty-two samples, including freshly prepared and ready-to-drink (RTD) infusions, syrups, concentrates, and an instant tea were evaluated by trained panelists, resulting in 21 defined and referenced descriptors, subsequently assembled in a sensory wheel. The vocabulary used by untrained panelists was investigated in Senegal, Portugal, France, and United Kingdom through professional meetings (n = 30), consumer focus groups (n = 75) and check-all-that-apply (CATA) studies (n = 490), resulting in graphical lexicons with 27 sensory and 15 overall impression terms. Training, food culture, and product familiarity influenced results. Trained panelists used precise terms to describe the aromatic profile of hibiscus teas (e.g., “reminiscent of dried hibiscus flowers,” with “berry,” “raisin,” “hay,” and “sweet” aromatic notes), whereas untrained ones were more prolific in general expressions (e.g., “strong in hibiscus” in Senegal; “floral” and “fruity” in Europe). Practical applications This work provides a foundation for sensory research on hibiscus teas and practical support for product optimization processes, whether through hibiscus cultivar selection and breeding, improvement of harvest and postharvest operations or tea processing methods. Moreover, new lexicons will facilitate communication between the actors involved in the development, production, and marketing of hibiscus extracts and products thereof across Africa, Europe, and North/South America. Finally, potential drivers of hibiscus tea acceptance in both traditional and new markets are also identified. (Resume d'auteur)

Our sense of taste enables us to detect the essential nutrients and toxic compounds present in our food. Sapid molecules, chemically very diverse, activate specialised receptors present in our oral cavity. The knowledge of the physiology of the perception of taste has progressed greatly over recent years and the main taste receptors are now known.; Le sens du goût permet de détecter les nutriments essentiels et des composés toxiques présents dans notre alimentation. Les molécules sapides, très diverses chimiquement, activent des détecteurs spécialisés présents dans notre cavité orale. La connaissance sur la physiologie de la perception du goût a fortement progressé durant ces dernières années et les principaux détecteurs gustatifs sont maintenant connus.

The role of 1-pyrroline was studied via feeding experiments using rice calli cultures to gain further insight into the key steps of 2-acetyl-1-pyrroline (2AP) biosynthesis in rice. The origin of the acetyl donor was also studied through stable isotope labelled substrates. Incubation of fresh calli from a fragrant rice variety (Aychade) and a non-fragrant variety (Gladio x Fidji K2) with 1-pyrroline led to a significant increase in 2AP in both varieties. Importantly, the amount of 2AP in the non-fragrant variety could be greatly enhanced by this supplementation. When rice calli were fed with increasing levels of 1-pyrroline, 2AP levels increased accordingly. Our data show that 1-pyrroline is a limiting factor for 2AP synthesis in rice. Heat treatment of calli suggested that 1-pyrroline might be enzymatically acetylated. The presence of labelled 2AP in calli supplemented with [U-C-13] glucose, sodium acetate (1,2-C-13(2)) and sodium octanoate (1,2,3,4-C-13(4)) suggested that these compounds are possible candidates for acetyl group-donors of 2AP, predominately in the form of intact labelled C-13(2)-units.

Aromatic quality of rice grains is known to vary greatly with environmental factors and cultivation methods. Among the environmental factors, soil salinity is thought to have a positive impact on the content of 2-acetyl-1-pyrroline (2AP) in grains, the key volatile compound of rice aroma. This study compared 2AP content in grains of three improved fragrant rice ( Oryza sativa L.) varieties grown in two fields, differing mainly in their soil salinity level. The impact of salinity on yield and main yield components was also investigated to understand the relationship between aromatic quality and yield build-up. Soil salinity was monitored by measuring the electrical conductivity (EC) of soil solution samples extracted every week. 2AP content in grains was determined by a newly developed stable isotope dilution method involving solid-phase microextraction (SPME) and GC MS/MS analysis. The results showed an increase of 2AP content in grains with salinity for the three varieties. The relationship between 2AP and mean EC of the crop fitted a single model for the three varieties ( R 2 = 0.728). In contrast, the impact of salinity on yield and yield components differed greatly between the three varieties. One variety appeared to be very sensitive to salt stress, with significant yield loss up to 40%, while the two other varieties proved to be resistant to the salinity levels experienced by the plants, with no significant yield loss or even higher yields in saline conditions. Nevertheless, the three varieties presented a significant negative correlation between 1000 grain weight (TGW) and the mean EC of the crop, and between TGW and 2AP content. It was concluded that the increase of 2AP content with salinity resulted partially from a 2AP concentration mechanism in smaller size grains. The direct effect of salinity on 2AP synthesis through stimulation of the proline metabolism is further discussed.

The purpose of this study was to determine how water and microbial stages in post-harvest processing affect the volatile content of coffee. Following our aroma analysis carried out on green coffees, we turned our attention to roasted coffees. Coffees produced by three variants of the wet method, and by an ecological process, were compared after roasting. Three degrees of roasting were applied to gain a clearer picture of the differences in roasted coffee aromas by means of a volatile compound analysis. Changes in the post-harvest process actually led to aroma differences in roasted coffees, and with light roasting it was possible to more effectively distinguish between the four treatments based on aroma criteria. Coffee produced by the traditional wet method, with microbial stages, had a better aroma quality than coffee produced by the ecological method, which was purely mechanical. Moreover, in the microbial method, mucilage removal under water gave coffees with more fruity, floral and caramel notes, whereas dry mucilage removal gave more neutral coffees. These results confirmed the importance of microbial mucilage removal under water and tallied with the results of our previous study on green coffee. This work provides coffee producers and roasters with an insight into the volatile composition of roasted coffee depending on the post-harvest process used and degree of roasting applied. © 2007 Elsevier Inc. All rights reserved.

Green coffees produced by three variants of the wet process and a new "ecological" process were characterised for their aroma using combined headspace solid-phase microextraction/gas chromatography-mass spectroscopy (HS-SPME/GC-MS) and headspace solidphase microextraction/gas chromatography-olfactometry (HS-SPME/GC-O). The effect of each post-harvest processing operation on the volatile fraction of the coffee produced was studied, particularly the effect of reducing the amount of water used in the process. The comparison of the green coffees from the different treatments revealed the importance of mucilage removal in distinguishing between the samples, and showed the merits of microbial mucilage removal in water to obtain coffees with a better aroma quality. These latter coffees were in fact characterised by pleasant and fruity aromatic notes, whereas those obtained after mechanical mucilage removal used in the ecological process were characterised by volatile compounds with an unpleasant note. © 2006 Elsevier Inc. All rights reserved.

This study describes a new and suitable method for the rapid evaluation of rice (Oryza sativa, L.) aroma by analysis of the volatile fraction using solid-phase microextraction coupled with mass spectrometry (SPME/MS). The abundance list of unresolved mass fragments of the SPME extracted volatile fraction formed the “fingerprint” of a rice sample. Fingerprints of 61 rice samples were recorded in duplicate. Pollutants originating from the extraction system induce fingerprint background that could be lowered by careful cleaning of vials and caps and by exclusion of specific mass fragments. A good discrimination between scented and nonscented rice samples was obtained using the SIMCA procedure. Most of the discriminating mass fragments could be directly or indirectly assigned to potential aromatic molecules present in rice. Keywords: Scented rice; aroma; 2-acetyl-1-pyrroline; solid-phase microextraction; mass spectrometry; fingerprint

Background The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors. Results Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by 1H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6 % of the isolates), it is Pestalotiopsis microspora (3.0 %) that increased the absolute amounts (quantified by 1H NMR in μmol/g DW green pods) of vanillin (37.0 × 10−3), vanillyl alcohol (100.0 × 10−3), vanillic acid (9.2 × 10−3) and p-hydroxybenzoic acid (87.9 × 10−3) by significant amounts. Conclusions All plants studied contained endophytic fungi and the isolation of the endophytes was conducted from plant organs at nine sites in Réunion Island including under shade house and undergrowth conditions. Endophytic variation occured between cultivation practices and the type of organ. Given the physical proximity of fungi inside pods, endophytic biotransformation may contribute to the complexity of vanilla flavors. Electronic supplementary material The online version of this article (doi:10.1186/s12870-015-0522-5) contains supplementary material, which is available to authorized users.

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The ascorbic acid content of orange juice made from concentrate was measured after 9 months of storage at 20 degrees C in glass, standard monolayer polyethylene terephthalate (PET1), multilayer PET (PET2) and plasma-treated PET (PET3) containers. Glass enabled the best preservation of ascorbic acid and, in plastic packaging materials, ascorbic acid losses were correlated with their oxygen permeability. PET2 and PET3, which exhibit oxygen permeability 10 times less than that of PET1, enabled a gain of 100 mg L-1 after 9 months of storage. Freshly hand-squeezed orange juice samples were adjusted to various pH values using sodium hydroxide; a rise in the pH from 3.2 to 4.0 significantly reduced the amounts of off-flavours (i.e., furfural and alpha-terpineol) appearing during storage, by 79% and 65%, respectively. Moreover, an increase in the pH from 3.2 to 4.0 enabled the protection of ascorbic acid levels without detrimentally increasing non-enzymatic browning.

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The evolution of aroma compounds from orange juice made from concentrate and stored in glass, standard monolayer polyethylene terephthalate (PET 1), multilayer PET (PET 2) and plasma-treated PET (internal carbon coating) (PET 3) was investigated. Bottles were stored at room temperature (20 degrees C) under artificial light. Volatile compounds in orange juice samples and corresponding packaging materials were analysed at zero time and after 2, 3 and 5 months of storage. After 5 months of storage, from 0.2 to 0.3% of the initial amounts of limonene and beta-myrcene in the orange juice had been absorbed by the plastic packaging materials. Statistical analyses showed that the evolution of aroma compounds was strongly correlated to the duration of storage, but not to the type of packaging material. Indeed, whatever the stored orange juice samples, the same evolutions were observed, with a decrease in aldehydes and ketones, esters, aliphatic alcohols, sesquiterpene and monoterpene alcohols, and an increase in two aliphatic and monoterpene alcohols (i.e. furfural and 4-vinylguaicol). The results suggest that the losses of aroma compounds from the juice could be attributed to the high acidity of the matrix, implying acid-catalysed reactions. Finally, PET packaging materials and their corresponding oxygen permeabilities showed no correlation with the loss of aroma compound.

The valorization of extracts from Brazilian vetiver (Vetiveria zizanioides (L.) Nash ex Small) roots was studied. This study took into account the extraction method, the chemical composition of the extracts, their sensorial characteristics, and the possibility of chemical transformations of the product. The performed extraction methods were hydrodistillation and extraction with supercritical carbon dioxide. Some pretreatment methods were tested on the vetiver roots and evaluated in terms of extraction yield, process time, chemical composition, and sensorial properties. Supercritical carbon dioxide extraction resulted in high yield (3.2%) in significantly less time than the other methods. The chemical compositions of the extracts obtained by the different methods were also compared to those of commercial vetiver oils from other sources, showing that Brazilian samples had a greater acid amount. An extraction in basic medium from Brazilian vetiver oil was done to remove its main acid (zizanoic acid), which was chemically transformed into an alcohol (khusimol) of desirable sensorial properties. Sensory evaluation indicated that the Brazilian volatile oil without acid could be used in perfumery and the extract obtained with supercritical carbon dioxide could have application in food.

The quantitative distribution of volatile compounds in the pulp, cloud, and serum of a freshly squeezed orange juice (cv. Naveline) was measured. Juice monoterpene and sesquiterpene hydrocarbons were primarily recovered from the pulp (74.0 and 87.2%, respectively) and cloud (7.3 and 14.9%, respectively). Esters and monoterpene alcohols were mainly found in the serum (90.4 and 84.1%, respectively). Long chain aliphatic aldehydes tend to concentrate in the pulp. The relative proportions of individual volatile compounds were similar in the pulp and cloud. Pulp and cloud alcohol insoluble residues exhibited similar compositions; half of them are made of nonwall proteins, and the rest are made of cell wall materials. Pulp and cloud total and neutral lipids had similar fatty acids distributions, although the cloud was much richer in total lipids than the pulp. No relationship was found between the retention of aroma compounds in the pulp or cloud and their AIR and lipid content or composition.

International audience; Amongst all kinds of coffee brews, “specialty coffee” is conspicuous in particular by a unique flavour. “Bourbon Pointu” coffee, Coffea arabica var. laurina, born from a spontaneous mutation of the Bourbon variety in Reunion Island, is a “specialty coffee”. This study aims at identifying potent odorants of “Bourbon Pointu” coffee brew according to its three trade classifications “Grand cru”, “Sublime” and “Authentique”. Solid phase extraction (SPE) permits to extract the volatiles. After their identification by gas chromatography–mass spectrometry (GC/MS), the potent odorants are determined by their odour activity value (OAV). The determination of their \OAV\ allowed one to differentiate coffee brews from the three trade classifications. Aromatic composition of “Bourbon Pointu” coffee is characterised by a predominance of aldehydes with high \OAV\ (e.g. (E,E)-nona-2,4-dienal: 13,600) for coffee brews belonging to “Grand cru” classification, of 2-phenylacetaldehyde (270) for coffee brews of “Sublime” classification and of a group of pyrazine compounds (e.g. 2-methyl-5-propylpyrazine: 16,750) for coffee brews of “Authentique” classification.

Introduction. A study on postharvest treatments of wild peppers was carried out in Madagascar with the aim of describing the local practices and measuring their impacts on the quality of the products. Materials and methods. Four distinct pepper production systems (PPS) were observed, described and compared in two separate areas in East Madagascar. Major quality characteristics (piperine and essential oil) of the peppercorns were assessed in samples collected in the four systems. Results and discussion. Two main postharvest processes (dry and wet) were identified. The wet process differed from the dry one in that it involved two specific operations, blanching and sweating. The processes influenced the color of the pepper. Piperine contents were not affected by any of the pepper production systems, whereas essential oil contents were reduced by up to 27% by the wet process. After processing, piperine contents were up to eight times lower, whereas essential oil contents were up to six times higher than the specifications of the standard ISO 959-1 for black pepper ready for commercialization. Conclusion. Two main processes (dry and wet) for treatment of peppercorns in Madagascar were identified and described. The dry process, with two steps less, appeared to be easier to implement and more respectful to the product. Improving maturity control and processing according to the quality expected by the markets will be necessary to promote Malagasy peppers.

El cacao venezolano es reconocido como cacao fino de aroma y la expresión de su potencial aromático depende en gran parte del tratamiento poscosecha que reciban las almendras. En este trabajo se identificaron y cuantificaron los com- puestos precursores del aroma presentes en el cacao proveniente de la zona de Chabasquén estado Portuguesa. Las almendras de cacao se fermentaron en cajo- nes cúbicos de madera de 60 cm de lado y posteriormente se secaron, en el sol. Los compuestos volátiles se extrajeron mediante la técnica de microextracción en fase sólida en cacao seco y tostado. Los resultados obtenidos permitieron identifi- car y cuantificar 60 compuestos en cacao seco y 108 en tostado distribuidos en 11 familias químicas y permitió evidenciar el efecto del tiempo de fermentación en el contenido de compuestos aromáticos. Finalmente, se pudo evidenciar la impor- tancia del manejo poscosecha en el cacao de Chabasquén, por lo tanto, se requiere establecer los mecanismos de transferencia a los productores de la región para garantizar un producto de buena calidad chocolatera.

Numerous aroma precursor evaluations have been undertaken with green coffee beans of both species of worldwide economic importance: Coffea arabica L. and Coffea canephora P. Efforts have been made to characterise cultivars of these two species. The originality of this study is to present the biochemical diversity of wild accessions originating from Ethiopia and Kenya for C. arabica (38 genotypes) and from five African countries (Cote d'Ivoire, Guinea, Congo, Cameroon and Central African Republik) for C. canephora (38 genotypes). The biochemical aroma parameters assessed by HPLC analysis were: (1) the two alkaloids, caffeine and trigonelline, (2) chlorogenic acids and (3) sucrose. Results reveal that the two species showed significant accession differences for all compounds. Between-species-average-content comparison confirms that C. arabica showed more trigonelline and sucrose and that C. canephora presented more CGA and caffeine. C. canephora diversity was higher than that of C. arabica, except for trigonelline and sucrose. For C. canephora, results showed that: (1) no differences were highlighted between accessions for countries of origin for the alkaloids and sucrose, and (2) the 3-CQA content allowed to accessions to be pooled into two groups.

Free and glycosidically-bound flavour compounds of acerola fruit were isolated and identified by GC and GC–MS analysis. Among the 46 compounds identified in the volatile fraction, the alcohols (3-methyl-but-3-en-1-ol, 3-methyl-butan-1-ol and 2-methyl-butan-1-ol) were predominant. Two other classes, aromatic compounds and esters, can participate in the fruity and fresh aroma of acerola. Among the 42 aglycones identified for the first time in this fruit, aliphatic alcohols and norisoprenoids were the main components. The latter were present in free form and only in traces. The hydrolysis of these aglycones could increase the fruity aroma of acerola. Four glucosides and one rutinoside were charaterized by GC–EIMS of their TFA derivatives.

The aim of this work was to compare the production of 6-pentyl-[alpha]-pyrone (6-PP), a compound which has a strong coconut-like aroma, by Trichoderma harzianum in liquid and in solid state cultivation (LC and SSC). The same liquid medium was used to impregnate sugarcane pith bagasse, used as support in SSC. The maximum concentration of 6-PP produced by T. harzianum in SSC was 2.8 mg (g dry cell mass) -1 equivalent to 0.9 g 1-1of impregnation medium, which is 17 times higher than that obtained in LC. The glucose consumed to yield 6-PP in SSC was 52 mg (g glucose) - 1, eight times higher than that found in LC. © 2000 Elsevier Science Ltd. All rights reserved. (Resume d'auteur)

International audience; Jumellea fragrans and Jumellea rossii, both named “Faham”, are two epiphytic orchids endemic to the Mascarene Islands (Reunion, Mauritius) widely used for their aromatic and medicinal properties. Cultivation of these orchids is currently non-existent, so gathering and poaching in natural populations provide the supply. Continued use of this resource and the reduction of its natural habitat now result in a drastic decline of its natural populations. In order to prevent the decline of these taxa, to preserve its natural evolution in the wild, and to answer to the local consumers demand, we initiate a multidisciplinary project. The main aim of the so-called “ORCHIFAH” project is to provide the scientific background requisite for the production of Faham at an industrial level. An ecological approach is set to characterise the environmental conditions (light, temperature, watering) for an optimal biomass production. In vitro conditions of asymbiotic germination are currently determined. The biochemical components variation of populations of the two species are analysed by RMN spectrometry. The development of an appropriated extractive mode of volatile compounds is planned. All this information will help us to accurately characterise the biology of Faham and the conditions of its production, essential prerequisites for the development of an agricultural production chain. The results will also allow us to determine the conservation status of the species and recommendations for the management of wild populations. The poster will explicit the different steps of the implementation of an agricultural production chain. The first results in each field will be presented.

In greenhouse experiments, Aychade, a fragrant rice variety, was grown under one level of salt solution (EC of 3800 ± 400 μS·cm(-1)) sufficient to induce salt stress in rice. Timing and duration of salt solution application varied according to the growth stages. 2-Acetyl-1-pyrroline (2AP), a characteristic flavor compound of fragrant rice as well as biogenetically related compounds, proline, and γ-aminobutyric acid (GABA) were quantified. Salt treatments induced 2AP synthesis in the leaves, but the increase was often higher in the vegetative phase. This increase was correlated with proline level but not with that of GABA. Interestingly the grains from all the salt treated plants contained significantly higher levels of 2AP (733-998 μg·kg(-1)) than those from the control (592 μg·kg(-1)). The highest 2AP synthesis occurred when the plants were subjected to salt treatment during whole vegetative or reproductive phases. However in the latter case crop yield decreased significantly.

Cocoa is an important raw material in international trade, for which the highest quality is demanded. Venezuelan Criollo cocoa beans from the south of Lake Maracaibo or Chuao are of very high quality and are considered to be Venezuela's finest beans. It is therefore important to characterise and define that quality, or its precursors, in the different primary cocoa processing stages. Given the large number of samples to be analysed, new analytical techniques providing fast and reliable quality data are essential. Near infrared (NIR) spectroscopy utilises wavelengths from 780–2500 nm to measure the absorbance by a sample, compute organic functional groups and quantitatively predict a particular factor. It has been extensively used to analyse food quality and to determine the main alkaloids of coffee. Fat, caffeine, theobromine and (−)-epicatechin contents are related to cocoa bean “flavour” quality. These parameters are usually determined using conventional methods, which are time consuming, destructive and expensive. The goals of this study were to use NIR spectroscopy to develop a fast and non-destructive tool to determine compounds in unfermented and sun-dried cocoa beans of high quality. Calibration coefficients of determination ( R2) and standard errors of cross-validation were 0.94 and 0.89%, 0.94 and 0.05%, 0.88 and 0.08% and 0.96 and 0.18% for fat, caffeine, theobromine and (−)-epicatechin contents (% dry matter), respectively. The results confirmed the good predictability of the models and showed that NIR spectroscopy can be used as a rapid method for determining these compounds in cocoa beans.