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In this paper we present visual methodologies attuned to the networked nature of digital images. First, we describe approaches to image research in which images are not separated from their network, but rather studied en groupe. Here, we contrast approaches that treat images as data, and those that regard images as content. Second, we focus on the production of images for digital research, presenting three of their functions: a) the creation of diagrams that facilitate collaboration in interdisciplinary research teams; b) the use of visualizations for cross-platform image analysis; and c) designing images for public participation. Most importantly, such visualizations are not used to form the esthetic culmination of analytical work, but are rather functional tools for digital research that serve parts of the entire research process, from its formulation and operationalization to the engagement of a broader public.

Live cell time-lapse microscopy, a widely-used technique to study gene expression and protein dynamics in single cells, relies on segmentation and tracking of individual cells for data generation. The potential of the data that can be extracted from this technique is limited by the inability to accurately segment a large number of cells from such microscopy images and track them over long periods of time. Existing segmentation and tracking algorithms either require additional dyes or markers specific to segmentation or they are highly specific to one imaging condition and cell morphology and/or necessitate manual correction. Here we introduce a fully automated, fast and robust segmentation and tracking algorithm for budding yeast that overcomes these limitations. Full automatization is achieved through a novel automated seeding method, which first generates coarse seeds, then automatically fine-tunes cell boundaries using these seeds and automatically corrects segmentation mistakes. Our algorithm can accurately segment and track individual yeast cells without any specific dye or biomarker. Moreover, we show how existing channels devoted to a biological process of interest can be used to improve the segmentation. The algorithm is versatile in that it accurately segments not only cycling cells with smooth elliptical shapes, but also cells with arbitrary morphologies (e.g. sporulating and pheromone treated cells). In addition, the algorithm is independent of the specific imaging method (bright-field/phase) and objective used (40X/63X/100X). We validate our algorithm’s performance on 9 cases each entailing a different imaging condition, objective magnification and/or cell morphology. Taken together, our algorithm presents a powerful segmentation and tracking tool that can be adapted to numerous budding yeast single-cell studies. [ABSTRACT FROM AUTHOR]

Macinnis' photographs of various groups of animals are so striking because all the animals assembled in front of the camera seem to be most willing to accept the camera's gaze and the power relation implied. Animals are usually hard to photograph, because they are not particularly collaborative, unpredictable in their movements, and tend to flee the frame. Macinnis' protagonists pose and look straight into the camera. They appear tame, pacified, 'civil', patiently awaiting their pictorial equivalent. As in all well-managed and representative group photos, there are no obvious signs of disorder or potential subversion. Macinnis' patchwork families look friendly and demonstrate unity and a sense of aesthetic order. Macinnis' photos allow for a reflection on group photographs and their specific arrangements. At the same time, they make one painfully aware of the disciplinary nature of the photographic act. Posing and freezing in front of the camera is a cultural practice that had to be trained and appropriated. Narratives from the beginnings of photography prove that. By looking at Macinnis' fully disciplined animal models, one realizes how much of our own unruliness we had to give up to fit into the photographic system. [ABSTRACT FROM AUTHOR]

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Multispectral LiDAR (light detection and ranging) data have been initially used for land cover classification. However, there are still high classification uncertainties, especially in urban areas, where objects are often mixed and confounded. This study investigated the efficiency of combining advanced statistical methods and LiDAR metrics derived from multispectral LiDAR data for improving land cover classification accuracy in urban areas. The study area is located in Oshawa, Ontario, Canada, on the Lake Ontario shoreline. Multispectral Optech Titan LiDAR data over the study area were acquired on 3 September 2014 in a single strip of 3 km2. Using the channels at 1,550 nm (C1), 1,064 nm (C2) and 532 nm (C3), LiDAR intensity data, normalized digital surface model (nDSM), pseudo normalized difference vegetation index (PseudoNDVI), morphological profiles (MP), and a novel hierarchical morphological profiles (HMP) were derived and used as features for the classification. A support vector machine classifier with a radial basis function (RBF) kernel was applied in the classification stage, where the optimal parameters for the classifier were selected by a grid search procedure. The combination of intensity, pseudoNDVI, nDSM and HMP resulted in the best land cover classification, with an overall accuracy of 93.28%. [ABSTRACT FROM AUTHOR]

The human face cues a wealth of social information, but the neural mechanisms that underpin social attributions from faces are not well known. In the current fMRI experiment, we used repetition suppression to test the hypothesis that populations of neurons in face perception and theory-of-mind neural networks would show sensitivity to faces that cue distinct trait judgments. Although faces were accurately discriminated based on associated traits, our results showed no evidence that face or theory-of-mind networks showed repetition suppression for face traits. Thus, we do not provide evidence for population coding models of face perception that include sensitivity to high and low trait features. Due to aspects of the experimental design, which bolstered statistical power and sensitivity, we have reasonable confidence that we could detect effects of a moderate size, should they exist. The null findings reported here, therefore, add value to models of neural organisation in social perception by showing instances where effects are absent or small. To test the generalisability of our findings, future work should test different types of trait judgment and different types of facial stimuli, in order to further probe the neurobiological bases of impression formation based on facial appearance. [ABSTRACT FROM AUTHOR]

Objective: To assess the diagnostic validity of clusters combining history elements and physical examination tests to diagnose partial or complete anterior cruciate ligament (ACL) tears. Design: Prospective diagnostic study. Settings: Orthopaedic clinics (n = 2), family medicine clinics (n = 2) and community-dwelling. Participants: Consecutive patients with a knee complaint (n = 279) and consulting one of the participating orthopaedic surgeons (n = 3) or sport medicine physicians (n = 2). Interventions: Not applicable. Main outcome measures: History elements and physical examination tests performed independently were compared to the reference standard: an expert physicians’ composite diagnosis including history elements, physical tests and confirmatory magnetic resonance imaging. Penalized logistic regression (LASSO) was used to identify history elements and physical examination tests associated with the diagnosis of ACL tear and recursive partitioning was used to develop diagnostic clusters. Diagnostic accuracy measures including sensitivity (Se), specificity (Sp), predictive values and positive and negative likelihood ratios (LR+/-) with associated 95% confidence intervals (CI) were calculated. Results: Forty-three individuals received a diagnosis of partial or complete ACL tear (15.4% of total cohort). The Lachman test alone was able to diagnose partial or complete ACL tears (LR+: 38.4; 95%CI: 16.0–92.5). Combining a history of trauma during a pivot with a “popping” sensation also reached a high diagnostic validity for partial or complete tears (LR+: 9.8; 95%CI: 5.6–17.3). Combining a history of trauma during a pivot, immediate effusion after trauma and a positive Lachman test was able to identify individuals with a complete ACL tear (LR+: 17.5; 95%CI: 9.8–31.5). Finally, combining a negative history of pivot or a negative popping sensation during trauma with a negative Lachman or pivot shift test was able to exclude both partial or complete ACL tears (LR-: 0.08; 95%CI: 0.03–0.24). Conclusion: Diagnostic clusters combining history elements and physical examination tests can support the differential diagnosis of ACL tears compared to various knee disorders. [ABSTRACT FROM AUTHOR]

Consistent with the view that disgust might be involved in persistent body dissatisfaction, there is preliminary evidence showing a positive correlation between measures of negative body image and indices of both trait disgust and self-directed disgust. In two correlational studies among undergraduates (N = 577 and N = 346, respectively) we aimed at replicating and extending these findings by testing a series of critical relationships, which follow from our hypotheses that 1) trait disgust propensity would increase the risk of developing a negative body image by increasing the likelihood of feeling self-disgust, and 2) trait disgust sensitivity would heighten the impact of self-disgust on the development of persistent negative body appraisals. Replicating previous research, both studies showed that negative body image was positively related to self-disgust, disgust propensity and disgust sensitivity. Mediation analyses showed that, in line with our model, self-disgust partly accounted for the association between disgust propensity and negative body image. Although disgust sensitivity showed an independent relationship with body image, disgust sensitivity did not moderate the association between self-disgust and negative body image. All in all, findings are consistent with the view that self-disgust-induced avoidance may contribute to persistent negative body appraisals. [ABSTRACT FROM AUTHOR]

Objective: To study the characterization of Fe3O4@Au-C225 composite targeted MNPs. Methods: Fe3O4@Au-C225 was prepared by the absorption method. The immunosorbent assay was used to evaluate its absorption efficiency at C225 Fc. ZETA SIZER3000 laser particle size analyzer, ultraviolet photometer and its characteristics were analyzed by VSM. the targeting effect of Fe3O4@Au-C225 composite targeted MNPs on U251 cells in vitro were detected by 7.0 Tesla Micro-MR; and subcutaneous transplanted human glioma in nude mice were performed the targeting effect in vivo after tail vein injection of Fe3O4@Au-C225 composite targeted MNPs by MRI. Results: The self-prepared Fe3O4@Au composite MNPs can adsorb C225 with high efficiency of adsorption so that Fe3O4@Au-C225 composite targeted MNPs were prepared successfully. Fe3O4@Au-C225 composite targeted MNPs favorably targeted human glioma cell line U251 in vitro; Fe3O4@Au-C225 composite targeted MNPs have good targeting ability to xenografted glioma on nude mice in vivo, and can be traced by MRI. Conclusion: The Fe3O4@Au-C225 composite targeted MNPs have the potential to be used as a tracer for glioma in vivo. [ABSTRACT FROM AUTHOR]

Along the anterior-posterior axis the central nervous system is subdivided into segmental units (neuromeres) the composition of which is adapted to their region-specific functional requirements. In Drosophila melanogaster each neuromere is formed by a specific set of identified neural stem cells (neuroblasts, NBs). In the thoracic and anterior abdominal region of the embryonic ventral nerve cord segmental sets of NBs resemble the ground state (2nd thoracic segment, which does not require input of homeotic genes), and serial (segmental) homologs generate similar types of lineages. The three gnathal head segments form a transitional zone between the brain and the ventral nerve cord. It has been shown recently that although all NBs of this zone are serial homologs of NBs in more posterior segments, they progressively differ from the ground state in anterior direction (labial > maxillary > mandibular segment) with regard to numbers and expression profiles. To study the consequences of their derived characters we traced the embryonic lineages of gnathal NBs using the Flybow and DiI-labelling techniques. For a number of clonal types serial homology is rather clearly reflected by their morphology (location and projection patterns) and cell specific markers, despite of reproducible segment-specific differences. However, many lineages, particularly in the mandibular segment, show a degree of derivation that impedes their assignment to ground state serial homologs. These findings demonstrate that differences in gene expression profiles of gnathal NBs go along with anteriorly directed progressive derivation in the composition of their lineages. Furthermore, lineage sizes decrease from labial to mandibular segments, which in concert with decreasing NB-numbers lead to reduced volumes of gnathal neuromeres, most significantly in the mandibular segment. [ABSTRACT FROM AUTHOR]

Pancreas injury by partial duct ligation (PDL) activates beta cell differentiation and proliferation in adult mouse pancreas but remains controversial regarding the anticipated increase in beta cell volume. Several reports unable to show beta cell volume augmentation in PDL pancreas used automated digital image analysis software. We hypothesized that fully automatic beta cell morphometry without manual micrograph artifact remediation introduces bias and therefore might be responsible for reported discrepancies and controversy. However, our present results prove that standard digital image processing with automatic thresholding is sufficiently robust albeit less sensitive and less adequate to demonstrate a significant increase in beta cell volume in PDL versus Sham-operated pancreas. We therefore conclude that other confounding factors such as quality of surgery, selection of samples based on relative abundance of the transcription factor Neurogenin 3 (Ngn3) and tissue processing give rise to inter-laboratory inconsistencies in beta cell volume quantification in PDL pancreas. [ABSTRACT FROM AUTHOR]

Early diagnosis of cartilage disease at a time when changes are limited to depletion of extracellular matrix components represents an important diagnostic target to reduce patient morbidity. This report is to present proof of concept for nanoparticle dependent cartilage barrier imaging in a large animal model including the use of clinical magnetic resonance imaging (MRI). Conditioned (following matrix depletion) and unconditioned porcine metacarpophalangeal cartilage was evaluated on the basis of fluorophore conjugated 30 nm and 80 nm spherical gold nanoparticle permeation and multiphoton laser scanning and bright field microscopy after autometallographic particle enhancement. Consequently, conditioned and unconditioned joints underwent MRI pre- and post-injection with 12 nm superparamagnetic iron oxide nanoparticles (SPIONs) to evaluate particle permeation in the context of matrix depletion and use of a clinical 1.5 Tesla MRI scanner. To gauge the potential pro-inflammatory effect of intra-articular nanoparticle delivery co-cultures of equine synovium and cartilage tissue were exposed to an escalating dose of SPIONs and IL-6, IL-10, IFN-γ and PGE2 were assessed in culture media. The chemotactic potential of growth media samples was subsequently assessed in transwell migration assays on isolated equine neutrophils. Results demonstrate an increase in MRI signal following conditioning of porcine joints which suggests that nanoparticle dependent compositional cartilage imaging is feasible. Tissue culture and neutrophil migration assays highlight a dose dependent inflammatory response following SPION exposure which at the imaging dose investigated was not different from controls. The preliminary safety and imaging data support the continued investigation of nanoparticle dependent compositional cartilage imaging. To our knowledge, this is the first report in using SPIONs as intra-articular MRI contrast agent for studying cartilage barrier function, which could potentially lead to a new diagnostic technique for early detection of cartilage disease. [ABSTRACT FROM AUTHOR]

The role of river discharge, wind and tide on the extension and variability of the Tagus River plume was analyzed from 2003 to 2015. This study was performed combining daily images obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor located onboard the Aqua and Terra satellites. Composites were generated by averaging pixels with the same forcing conditions. River discharge shows a strong relation with the extension of the Tagus plume. The plume grows with the increasing river discharge and express a two day lag caused by the long residence time of water within the estuary. The Tagus turbid plume was found to be smaller under northerly and easterly winds, than under southerly and westerly winds. It is suggested that upwelling favoring winds provoke the offshore movement of the plume material with a rapidly decrease in turbidity values whereas downwelling favoring winds retain plume material in the north coast close to the Tagus mouth. Eastern cross-shore (oceanward) winds spread the plume seaward and to the north following the coast geometry, whereas western cross-shore (landward) winds keep the plume material in both alongshore directions occupying a large part of the area enclosed by the bay. Low tides produce larger and more turbid plumes than high tides. In terms of fortnightly periodicity, the maximum plume extension corresponding to the highest turbidity is observed during and after spring tides. Minimum plume extension associated with the lowest turbidity occurs during and after neap tides. [ABSTRACT FROM AUTHOR]

For the period 1996-2010, we provide the first indication of the drivers behind mangrove land cover and land use change across the (pan-)tropics using time-series Japanese Earth Resources Satellite (JERS-1) Synthetic Aperture Radar (SAR) and Advanced Land Observing Satellite (ALOS) Phased Array-type L-band SAR (PALSAR) data. Multi-temporal radar mosaics were manually interpreted for evidence of loss and gain in forest extent and its associated driver. Mangrove loss as a consequence of human activities was observed across their entire range. Between 1996-2010 12% of the 1168 1°x1° radar mosaic tiles examined contained evidence of mangrove loss, as a consequence of anthropogenic degradation, with this increasing to 38% when combined with evidence of anthropogenic activity prior to 1996. The greatest proportion of loss was observed in Southeast Asia, whereby approximately 50% of the tiles in the region contained evidence of mangrove loss, corresponding to 18.4% of the global mangrove forest tiles. Southeast Asia contained the greatest proportion (33.8%) of global mangrove forest. The primary driver of anthropogenic mangrove loss was found to be the conversion of mangrove to aquaculture/agriculture, although substantial advance of mangroves was also evident in many regions. [ABSTRACT FROM AUTHOR]

An automated approach based on routinely-processed, whole-slide immunohistochemistry (IHC) was implemented to study co-localized protein expression in tissue samples. Expression of two markers was chosen to represent stromal (CD31) and epithelial (Ki-67) compartments in prostate cancer. IHC was performed on whole-slide sections representing low-, intermediate-, and high-grade disease from 15 patients. The automated workflow was developed using a training set of regions-of-interest in sequential tissue sections. Protein expression was studied on digital representations of IHC images across entire slides representing formalin-fixed paraffin embedded blocks. Using the training-set, the known association between Ki-67 and Gleason grade was confirmed. CD31 expression was more heterogeneous across samples and remained invariant with grade in this cohort. Interestingly, the Ki-67/CD31 ratio was significantly increased in high (Gleason ≥ 8) versus low/intermediate (Gleason ≤7) samples when assessed in the training-set and the whole-tissue block images. Further, the feasibility of the automated approach to process Tissue Microarray (TMA) samples in high throughput was evaluated. This work establishes an initial framework for automated analysis of co-localized protein expression and distribution in high-resolution digital microscopy images based on standard IHC techniques. Applied to a larger sample population, the approach may help to elucidate the biologic basis for the Gleason grade, which is the strongest, single factor distinguishing clinically aggressive from indolent prostate cancer. [ABSTRACT FROM AUTHOR]

Lichens form a critical portion of barren ground caribou (Rangifer tarandus groenlandicus) diets, especially during winter months. Here, we assess lichen mat volume across five herd ranges in the Northwest Territories and Nunavut, Canada, using newly developed composite Landsat imagery. The lichen volume estimator (LVE) was adapted for use across 700 000 km2 of barren ground caribou habitat annually from 1984–2012. We subsequently assessed how LVE changed temporally throughout the time series for each pixel using Theil-Sen’s slopes, and spatially by assessing whether slope values were centered in local clusters of similar values. Additionally, we assessed how LVE estimates resulted in changes in barren ground caribou movement rates using an extensive telemetry data set from 2006–2011. The Ahiak/Beverly herd had the largest overall increase in LVE (median = 0.033), while the more western herds had the least (median slopes below zero in all cases). LVE slope pixels were arranged in significant clusters across the study area, with the Cape Bathurst, Bathurst, and Bluenose East herds having the most significant clusters of negative slopes (more than 20% of vegetated land in each case). The Ahiak/Beverly and Bluenose West had the most significant positive clusters (16.3% and 18.5% of vegetated land respectively). Barren ground caribou displayed complex reactions to changing lichen conditions depending on season; the majority of detected associations with movement data agreed with current understanding of barren ground caribou foraging behavior (the exception was an increase in movement velocity at high lichen volume estimates in Fall). The temporal assessment of LVE identified areas where shifts in ecological conditions may have resulted in changing lichen mat conditions, while assessing the slope estimates for clustering identified zones beyond the pixel scale where forage conditions may be changing. Lichen volume estimates associated with barren ground caribou movement metrics in an expected manner and, as such, show value for future habitat assessments. [ABSTRACT FROM AUTHOR]

In this paper, a multifractal downscaling technique is applied to adequately transformed and lag corrected normalized difference vegetation index (NDVI) in order to obtain daily estimates of rainfall in an area of the Peruvian Andean high plateau. This downscaling procedure is temporal in nature since the original NDVI information is provided at an irregular temporal sampling period between 8 and 11 days, and the desired final scale is 1 day. The spatial resolution of approximately 1 km remains the same throughout the downscaling process. The results were validated against on-site measurements of meteorological stations distributed in the area under study. [ABSTRACT FROM AUTHOR]

Elastography, the imaging of elastic properties of soft tissues, is well developed for macroscopic clinical imaging of soft tissues and can provide useful information about various pathological processes which is complementary to that provided by the original modality. Scaling down of this technique should ply the field of cellular biology with valuable information with regard to elastic properties of cells and their environment. This paper evaluates the potential to develop such a tool by modifying a commercial optical coherence tomography (OCT) device to measure the speed of shear waves propagating in a three-dimensional (3D) medium. A needle, embedded in the gel, was excited to vibrate along its long axis and the displacement as a function of time and distance from the needle associated with the resulting shear waves was detected using four M-mode images acquired simultaneously using a commercial four-channel swept-source OCT system. Shear-wave time of arrival (TOA) was detected by tracking the axial OCT-speckle motion using cross-correlation methods. Shear-wave speed was then calculated from inter-channel differences of TOA for a single burst (the relative TOA method) and compared with the shear-wave speed determined from positional differences of TOA for a single channel over multiple bursts (the absolute TOA method). For homogeneous gels the relative method provided shear-wave speed with acceptable precision and accuracy when judged against the expected linear dependence of shear modulus on gelatine concentration (R2 = 0.95) and ultimate resolution capabilities limited by 184μm inter-channel distance. This overall approach shows promise for its eventual provision as a research tool in cancer cell biology. Further work is required to optimize parameters such as vibration frequency, burst length and amplitude, and to assess the lateral and axial resolutions of this type of device as well as to create 3D elastograms. [ABSTRACT FROM AUTHOR]

Understanding the warming trends at local level is critical; and, the development of relevant adaptation and mitigation policies at those levels are quite challenging. Here, our overall goal was to generate local warming trend map at 1 km spatial resolution by using: (i) Moderate Resolution Imaging Spectroradiometer (MODIS)-based 8-day composite surface temperature data; (ii) weather station-based yearly average air temperature data; and (iii) air temperature normal (i.e., 30 year average) data over the Canadian province of Alberta during the period 1961–2010. Thus, we analysed the station-based air temperature data in generating relationships between air temperature normal and yearly average air temperature in order to facilitate the selection of year-specific MODIS-based surface temperature data. These MODIS data in conjunction with weather station-based air temperature normal data were then used to model local warming trends. We observed that almost 88% areas of the province experienced warming trends (i.e., up to 1.5°C). The study concluded that remote sensing technology could be useful for delineating generic trends associated with local warming. [ABSTRACT FROM AUTHOR]

Mapping the distribution of forested areas and monitoring their spatio-temporal changes are necessary for the conservation and management of forests. This paper presents two new image composites for the visualization and extraction of forest cover. By exploiting the Landsat-8 satellite-based multi-temporal and multi-spectral reflectance datasets, the Forest Cover Composite (FCC) was designed in this research. The FCC is an RGB (red, green, blue) color composite made up of short-wave infrared reflectance and green reflectance, specially selected from the day when the Normalized Difference Vegetation Index (NDVI) is at a maximum, as the red and blue bands, respectively. The annual mean NDVI values are used as the green band. The FCC is designed in such a way that the forested areas appear greener than other vegetation types, such as grasses and shrubs. On the other hand, the croplands and barren lands are usually seen as red and water/snow is seen as blue. However, forests may not necessarily be greener than other perennial vegetation. To cope with this problem, an Enhanced Forest Cover Composite (EFCC) was designed by combining the annual median backscattering intensity of the VH (vertical transmit, horizontal receive) polarization data from the Sentinel-1 satellite with the green term of the FCC to suppress the green component (mean NDVI values) of the FCC over the non-forested vegetative areas. The performances of the FCC and EFCC were evaluated for the discrimination and classification of forested areas all over Japan with the support of reference data. The FCC and EFCC provided promising results, and the high-resolution forest map newly produced in the research provided better accuracy than the extant MODIS (Moderate Resolution Imaging Spectroradiometer) Land Cover Type product (MCD12Q1) in Japan. The composite images proposed in the research are expected to improve forest monitoring activities in other regions as well.

During placental malaria, Plasmodium falciparum infected erythrocytes sequester in the placenta, causing health problems for both the mother and fetus. The specific adherence is mediated by the VAR2CSA protein, which binds to placental chondroitin sulfate (CS) on chondroitin sulfate proteoglycans (CSPGs) in the placental syncytium. However, the identity of the CSPG core protein and the cellular impact of the interaction have remain elusive. In this study we identified the specific CSPG core protein to which the CS is attached, and characterized its exact placental location. VAR2CSA pull-down experiments using placental extracts from whole placenta or syncytiotrophoblast microvillous cell membranes showed three distinct CSPGs available for VAR2CSA adherence. Further examination of these three CSPGs by immunofluorescence and proximity ligation assays showed that syndecan-1 is the main receptor for VAR2CSA mediated placental adherence. We further show that the commonly used placental choriocarcinoma cell line, BeWo, express a different set of proteoglycans than those present on placental syncytiotrophoblast and may not be the most biologically relevant model to study placental malaria. Syncytial fusion of the BeWo cells, triggered by forskolin treatment, caused an increased expression of placental CS-modified syndecan-1. In line with this, we show that rVAR2 binding to placental CS impairs syndecan-1-related Src signaling in forskolin treated BeWo cells, but not in untreated cells. [ABSTRACT FROM AUTHOR]

Effective and timely quantification of the spatiotemporal pattern of urban expansion in China is important for the assessment of its environmental effects. However, the dynamics of the most recent urban expansions in China since 2012 have not yet been adequately explained due to a lack of current information. In this paper, our objective was to quantify spatiotemporal patterns of urban expansion in China between 1992 and 2015. First, we extracted information on urban expansion in China between 1992 and 2015 by integrating nighttime light data, vegetation index data, and land surface temperature data. Then we analyzed the spatiotemporal patterns of urban expansion at the national and regional scales, as well as at that of urban agglomerations. We found that China experienced a rapid and large-scale process of urban expansion between 1992 and 2015, with urban land increasing from 1.22 × 104 km2 to 7.29 × 104 km2, increasing in size nearly fivefold and with an average annual growth rate of 8.10%, almost 2.5 times as rapid as the global average. We also found that urban land in China expanded mainly by occupying 3.31 × 104 km2 of cropland, which comprised 54.67% of the total area of expanded urban land. Among the three modes of growth—infilling, edge expansion, and leapfrog—edge expansion was the main cause of cropland loss. Cropland loss resulting from edge expansion of urban land totalled 2.51 × 104 km2, accounting for over 75% of total cropland loss. We suggest that effective future management with respect to edge expansion of urban land is needed to protect cropland in China. [ABSTRACT FROM AUTHOR]

The extracellular matrix (ECM) is a pivotal component adult tissues and of many tissue-specific stem cell niches. It provides structural support and regulates niche signaling during tissue maintenance and regeneration. In many tissues, ECM remodeling depends on the regulation of MMP (atrix etalloroteinase) activity by inhibitory TIMP (issue nhibitors of metalloproteinases) proteins. Here, we report that the only Drosophila timp gene is required for maintaining the normal organization and function of the germline stem cell niche in adult females. timp mutant ovaries show reduced levels of both Drosophila Collagen IV α chains. In addition, tissue stiffness and the cellular organization of the ovarian niche are affected in timp mutants. Finally, loss of timp impairs the ability of the germline stem cell niche to generate new cysts. Our results demonstrating a crucial role for timp in tissue organization and gamete production thus provide a link between the regulation of ECM metabolism and tissue homeostasis. [ABSTRACT FROM AUTHOR]

The beetle Tribolium castaneum has increasingly become a powerful model for comparative research on insect development. One recent resource is a collection of piggyBac transposon-based enhancer trap lines. Here, we provide a detailed analysis of three selected lines and demonstrate their value for investigations in the second half of embryogenesis, which has thus far lagged behind research on early stages. Two lines, G12424 and KT650, show enhanced green fluorescent protein (EGFP) expression throughout the extraembryonic serosal tissue and in a few discrete embryonic domains. Intriguingly, both lines show for the first time a degree of regionalization within the mature serosa. However, their expression profiles illuminate distinct aspects of serosal biology: G12424 tracks the tissue’s rapid maturation while KT650 expression likely reflects ongoing physiological processes. The third line, G04609, is stably expressed in mesodermal domains, including segmental muscles and the heart. Genomic mapping followed by in situ hybridization for genes near to the G04609 insertion site suggests that the transposon has trapped enhancer information for the Tribolium orthologue of midline (Tc-mid). Altogether, our analyses provide the first live imaging, long-term characterizations of enhancer traps from this collection. We show that EGFP expression is readily detected, including in heterozygote crosses that permit the simultaneous visualization of multiple tissue types. The tissue specificity provides live, endogenous marker gene expression at key developmental stages that are inaccessible for whole mount staining. Furthermore, the nonlocalized EGFP in these lines illuminates both the nucleus and cytoplasm, providing cellular resolution for morphogenesis research on processes such as dorsal closure and heart formation. In future work, identification of regulatory regions driving these enhancer traps will deepen our understanding of late developmental control, including in the extraembryonic domain, which is a hallmark of insect development but which is not yet well understood. [ABSTRACT FROM AUTHOR]

The beetle Tribolium castaneum has increasingly become a powerful model for comparative research on insect development. One recent resource is a collection of piggyBac transposon-based enhancer trap lines. Here, we provide a detailed analysis of three selected lines and demonstrate their value for investigations in the second half of embryogenesis, which has thus far lagged behind research on early stages. Two lines, G12424 and KT650, show enhanced green fluorescent protein (EGFP) expression throughout the extraembryonic serosal tissue and in a few discrete embryonic domains. Intriguingly, both lines show for the first time a degree of regionalization within the mature serosa. However, their expression profiles illuminate distinct aspects of serosal biology: G12424 tracks the tissue’s rapid maturation while KT650 expression likely reflects ongoing physiological processes. The third line, G04609, is stably expressed in mesodermal domains, including segmental muscles and the heart. Genomic mapping followed by in situ hybridization for genes near to the G04609 insertion site suggests that the transposon has trapped enhancer information for the Tribolium orthologue of midline (Tc-mid). Altogether, our analyses provide the first live imaging, long-term characterizations of enhancer traps from this collection. We show that EGFP expression is readily detected, including in heterozygote crosses that permit the simultaneous visualization of multiple tissue types. The tissue specificity provides live, endogenous marker gene expression at key developmental stages that are inaccessible for whole mount staining. Furthermore, the nonlocalized EGFP in these lines illuminates both the nucleus and cytoplasm, providing cellular resolution for morphogenesis research on processes such as dorsal closure and heart formation. In future work, identification of regulatory regions driving these enhancer traps will deepen our understanding of late developmental control, including in the extraembryonic domain, which is a hallmark of insect development but which is not yet well understood. [ABSTRACT FROM AUTHOR]

Abstract: Peripheral MR angiography requires high resolution and arterial contrast. Neither can be obtained simultaneously due to the short arterial phase of the contrast agent. To improve temporal resolution, keyhole imaging was developed, which combines high resolution and arterial k-spaces at the time of image acquisition. Here, a related approach is introduced for image post-processing in the Fourier domain. It is demonstrated that simple substitution of the central k-space with low-resolution data leads to severe distortion. Hence, a dedicated calculation scheme is necessary for composite k-space post-processing. A solution is presented for high-resolution arterial peripheral MR angiography that uses subtraction of venous intensities from the central high-resolution k-space. The calculations in the Fourier domain do not require interpolations between the different resolutions. High-resolution steady-state MR angiography, which exhibits contrast-enhanced arteries and veins at an isotropic resolution of 0.65 mm, and standard resolution arterial first-pass MR angiography were combined to obtain images with the resolution of the steady-state images and arterial contrast. Numerical simulations on software phantoms are presented. The operation of the method is demonstrated in five patients. [Copyright &y& Elsevier]

A polymodelling system (POLYMOD) is presented for varied 2D or 3D displays of chemical shapes, whether structural (molecular volumes distances, surfaces...) or electronic (molecular electrostatic potential, electron densities ...). The POLYMOD system manages reversible correspondences between calculation and representation spaces by immersing the molecules studied in a unique represetation space structured by a 3D grid box. This box, called COOBOX, defined by its point internal coordinates, is used to transform other coordinates into its own. Direct multi-slice images along the x, y, z axis provide access to a variety of derived operations around a molecular shape or set of shapes disposed in various manners. Interactive sub systems ensure Boolean operations and compare property shapes through the 3D grid. Clear separation of graphic routine and of downstream modelling programs leads to rapid and original generation of varied images (subimages, direct or composite images) which are then available for simulation strategies. [ABSTRACT FROM AUTHOR]