1. Development of affinity-based delivery of NGF from a chondroitin sulfate biomaterial
- Author
-
Butterfield, Karen Chao, Conovaloff, Aaron W, and Panitch, Alyssa
- Subjects
Engineering ,Biomedical Engineering ,Biotechnology ,Neurosciences ,5.2 Cellular and gene therapies ,Development of treatments and therapeutic interventions ,Animals ,Binding Sites ,Biocompatible Materials ,Brain ,Chondroitin Sulfates ,Drug Delivery Systems ,Humans ,Hydrogels ,Nerve Growth Factor ,Nerve Regeneration ,Neurites ,Peptides ,Polyethylene Glycols ,Rats ,Sulfates ,Tissue Engineering ,chondroitin sulfate ,controlled release ,hydrogel ,nerve growth factor ,nerve regeneration ,spinal cord injury - Abstract
Chondroitin sulfate is a major component of the extracellular matrix in both the central and peripheral nervous systems. Chondroitin sulfate is upregulated at injury, thus methods to promote neurite extension through chondroitin sulfate-rich matrices and synthetic scaffolds are needed. We describe the use of both chondroitin sulfate and a novel chondroitin sulfate-binding peptide to control the release of nerve growth factor. Interestingly, the novel chondroitin sulfate-binding peptide enhances the controlled release properties of the chondroitin sulfate gels. While introduction of chondroitin sulfate into a scaffold inhibits primary cortical outgrowth, the combination of chondroitin sulfate, chondroitin sulfate-binding peptide and nerve growth factor promotes primary cortical neurite outgrowth in chondroitin sulfate gels.
- Published
- 2011