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3. Practice patterns and 90-day treatment-related morbidity in early-stage cervical cancer

Author

Bogani, G, Donato, V, Scambia, G, Landoni, F, Ghezzi, F, Muzii, L, Panici, P, Raspagliesi, F, Casarin, J, Di Martino, G, Grassi, T, Perrone, A, De Iaco, P, Multinu, F, Berretta, R, Capozzi, V, Zupi, E, Centini, G, Pellegrino, A, Corso, S, Stevenazzi, G, Boschi, A, Comerci, G, Greco, P, Scutiero, G, Sopracordevole, F, Giorda, G, Fichera, M, Simoncini, T, Caretto, M, Sartori, E, Ferrari, F, Cianci, A, Sarpietro, G, Matarazzo, M, Giampaolino, P, Bifulco, G, Morelli, M, Dio, M, Ferrero, A, Biglia, N, Barra, F, Ferrero, S, Cianci, S, Chiantera, V, Sozzi, G, Ercoli, A, Schettini, S, Orlando, T, Cannone, F, Ettore, G, Puppo, A, Olearo, E, Leone Roberti Maggiore, U, Artuso, V, Palaia, I, Perniola, G, Tripodi, R, D'Auge, T, Cuccu, I, Fischetti, M, Santangelo, G, Casorelli, A, Giannini, A, D'Oria, O, Vizzielli, G, Restaino, S, Bergamini, A, Bocciolone, L, Plotti, F, Angioli, R, Mantovani, G, Ceccaroni, M, Cassini, C, Dominoni, M, Giambanco, L, Amodeo, S, Leo, L, Thommaset, R, Raimondo, D, Seracchioli, R, Malzoni, M, Falcone, F, Gorlero, F, Di Luca, M, Busato, E, Kilzie, S, Dell'Acqua, A, Scarfone, G, Vercellini, P, Petrillo, M, Capobianco, G, Ciavattini, A, Mereu, L, Scollo, P, Sorbi, F, Fambrini, M, Romano, F, Ricci, G, Trojano, G, Damiani, G, Consonni, R, Di Lorenzo, N, Lippolis, A, Tinelli, R, Aguzzoli, L, Mandato, V, Palomba, S, Tripodi, M, Calandra, D, Pellegrini, F, Zullo, F, Surico, D, Remorgida, V, Ruscitto, F, Beretta, P, Vizza, E, Bogani G., Donato V. D., Scambia G., Landoni F., Ghezzi F., Muzii L., Panici P. B., Raspagliesi F., Casarin J., Di Martino G., Grassi T., Perrone A. M., De Iaco P., Multinu F., Berretta R., Capozzi V. A., Zupi E., Centini G., Pellegrino A., Corso S., Stevenazzi G., Boschi A. C., Comerci G., Greco P., Scutiero G., Sopracordevole F., Giorda G., Fichera M., Simoncini T., Caretto M., Sartori E., Ferrari F., Cianci A., Sarpietro G., Matarazzo M. G., Giampaolino P., Bifulco G., Morelli M., Dio M. D., Ferrero A., Biglia N., Barra F., Ferrero S., Cianci S., Chiantera V., Sozzi G., Ercoli A., Schettini S., Orlando T., Cannone F. G., Ettore G., Puppo A., Olearo E., Leone Roberti Maggiore U., Artuso V., Palaia I., Perniola G., Tripodi R., D'Auge T. G., Cuccu I., Fischetti M., Santangelo G., Casorelli A., Giannini A., D'Oria O., Vizzielli G., Restaino S., Bergamini A., Bocciolone L., Plotti F., Angioli R., Mantovani G., Ceccaroni M., Cassini C., Dominoni M., Giambanco L., Amodeo S., Leo L., Thommaset R., Raimondo D., Seracchioli R., Malzoni M., Falcone F., Gorlero F., Di Luca M., Busato E., Kilzie S., Dell'Acqua A., Scarfone G., Vercellini P., Petrillo M., Capobianco G., Ciavattini A., Mereu L., Scollo P., Sorbi F., Fambrini M., Romano F., Ricci G., Trojano G., Damiani G. R., Consonni R., Di Lorenzo N., Lippolis A., Tinelli R., Aguzzoli L., Mandato V. D., Palomba S., Tripodi M., Calandra D., Pellegrini F., Zullo F., Surico D., Remorgida V., Ruscitto F., Beretta P., Vizza E., Bogani, G, Donato, V, Scambia, G, Landoni, F, Ghezzi, F, Muzii, L, Panici, P, Raspagliesi, F, Casarin, J, Di Martino, G, Grassi, T, Perrone, A, De Iaco, P, Multinu, F, Berretta, R, Capozzi, V, Zupi, E, Centini, G, Pellegrino, A, Corso, S, Stevenazzi, G, Boschi, A, Comerci, G, Greco, P, Scutiero, G, Sopracordevole, F, Giorda, G, Fichera, M, Simoncini, T, Caretto, M, Sartori, E, Ferrari, F, Cianci, A, Sarpietro, G, Matarazzo, M, Giampaolino, P, Bifulco, G, Morelli, M, Dio, M, Ferrero, A, Biglia, N, Barra, F, Ferrero, S, Cianci, S, Chiantera, V, Sozzi, G, Ercoli, A, Schettini, S, Orlando, T, Cannone, F, Ettore, G, Puppo, A, Olearo, E, Leone Roberti Maggiore, U, Artuso, V, Palaia, I, Perniola, G, Tripodi, R, D'Auge, T, Cuccu, I, Fischetti, M, Santangelo, G, Casorelli, A, Giannini, A, D'Oria, O, Vizzielli, G, Restaino, S, Bergamini, A, Bocciolone, L, Plotti, F, Angioli, R, Mantovani, G, Ceccaroni, M, Cassini, C, Dominoni, M, Giambanco, L, Amodeo, S, Leo, L, Thommaset, R, Raimondo, D, Seracchioli, R, Malzoni, M, Falcone, F, Gorlero, F, Di Luca, M, Busato, E, Kilzie, S, Dell'Acqua, A, Scarfone, G, Vercellini, P, Petrillo, M, Capobianco, G, Ciavattini, A, Mereu, L, Scollo, P, Sorbi, F, Fambrini, M, Romano, F, Ricci, G, Trojano, G, Damiani, G, Consonni, R, Di Lorenzo, N, Lippolis, A, Tinelli, R, Aguzzoli, L, Mandato, V, Palomba, S, Tripodi, M, Calandra, D, Pellegrini, F, Zullo, F, Surico, D, Remorgida, V, Ruscitto, F, Beretta, P, Vizza, E, Bogani G., Donato V. D., Scambia G., Landoni F., Ghezzi F., Muzii L., Panici P. B., Raspagliesi F., Casarin J., Di Martino G., Grassi T., Perrone A. M., De Iaco P., Multinu F., Berretta R., Capozzi V. A., Zupi E., Centini G., Pellegrino A., Corso S., Stevenazzi G., Boschi A. C., Comerci G., Greco P., Scutiero G., Sopracordevole F., Giorda G., Fichera M., Simoncini T., Caretto M., Sartori E., Ferrari F., Cianci A., Sarpietro G., Matarazzo M. G., Giampaolino P., Bifulco G., Morelli M., Dio M. D., Ferrero A., Biglia N., Barra F., Ferrero S., Cianci S., Chiantera V., Sozzi G., Ercoli A., Schettini S., Orlando T., Cannone F. G., Ettore G., Puppo A., Olearo E., Leone Roberti Maggiore U., Artuso V., Palaia I., Perniola G., Tripodi R., D'Auge T. G., Cuccu I., Fischetti M., Santangelo G., Casorelli A., Giannini A., D'Oria O., Vizzielli G., Restaino S., Bergamini A., Bocciolone L., Plotti F., Angioli R., Mantovani G., Ceccaroni M., Cassini C., Dominoni M., Giambanco L., Amodeo S., Leo L., Thommaset R., Raimondo D., Seracchioli R., Malzoni M., Falcone F., Gorlero F., Di Luca M., Busato E., Kilzie S., Dell'Acqua A., Scarfone G., Vercellini P., Petrillo M., Capobianco G., Ciavattini A., Mereu L., Scollo P., Sorbi F., Fambrini M., Romano F., Ricci G., Trojano G., Damiani G. R., Consonni R., Di Lorenzo N., Lippolis A., Tinelli R., Aguzzoli L., Mandato V. D., Palomba S., Tripodi M., Calandra D., Pellegrini F., Zullo F., Surico D., Remorgida V., Ruscitto F., Beretta P., and Vizza E.

Abstract

Background: To evaluate the impact of the Laparoscopic Approach to Cervical Cancer (LACC) Trial on patterns of care and surgery-related morbidity in early-stage cervical cancer. Methods: This is a retrospective, a multi-institutional study evaluating 90-day surgery-related outcomes of patients undergoing treatment for early-stage cervical cancer before (period I: 01/01/2016–06/01/2018) and after (period II: 01/01/2019–06/01/2021) the publication of the results of the LACC trial. Results: Charts of 1295 patients were evaluated: 581 (44.9%) and 714 (55.1%) before and after the publication of the LACC trial, respectively. After the publication of the LACC trial, the number of patients treated with minimally invasive radical hysterectomy decreased from 64.9% to 30.4% (p < 0.001). Overall, 90-day complications occurred in 110 (18.9%) and 119 (16.6%) patients in the period I and period II, respectively (p = 0.795). Similarly, the number of severe (grade 3 or worse) complications did not differ between the two periods (38 (6.5%) vs. 37 (5.1%); p = 0.297). Overall and severe 90-day complications were consistent between periods even evaluating stage IA (p = 0.471), IB1 (p = 0.929), and IB2 (p = 0.074), separately. Conclusions: The present investigation highlighted that in referral centers the shift from minimally invasive to open radical hysterectomy does not influence 90-day surgery-related morbidity.

Published
2022

6. A Contribution to the Harmonization of Non-targeted NMR Methods for Data-Driven Food Authenticity Assessment

Author

Gallo, V, Ragone, R, Musio, B, Todisco, S, Rizzuti, A, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Pascazio, A, Cobas, C, Mari, S, Garino, C, Arlorio, M, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Barison, A, Benevelli, F, Borioni, A, Cagliani, L, Casadei, L, Marincola, F, Colson, K, Consonni, R, Costantino, G, Cremonini, M, Davalli, S, Duarte, I, Guyader, S, Hamon, E, Hegmanns, M, Lamanna, R, Longobardi, F, Mallamace, D, Mammi, S, Markus, M, Menezes, L, Milone, S, Molero-Vilchez, D, Mucci, A, Napoli, C, Rossi, M, Saez-Barajas, E, Savorani, F, Schievano, E, Sciubba, F, Sobolev, A, Takis, P, Thomas, F, Villa-Valverde, P, Latronico, M, Gallo V., Ragone R., Musio B., Todisco S., Rizzuti A., Mastrorilli P., Pontrelli S., Intini N., Scapicchio P., Triggiani M., Pascazio A., Cobas C., Mari S., Garino C., Arlorio M., Acquotti D., Airoldi C., Arnesano F., Assfalg M., Barison A., Benevelli F., Borioni A., Cagliani L. R., Casadei L., Marincola F. C., Colson K., Consonni R., Costantino G., Cremonini M. A., Davalli S., Duarte I., Guyader S., Hamon E., Hegmanns M., Lamanna R., Longobardi F., Mallamace D., Mammi S., Markus M., Menezes L. R. A., Milone S., Molero-Vilchez D., Mucci A., Napoli C., Rossi M. C., Saez-Barajas E., Savorani F., Schievano E., Sciubba F., Sobolev A., Takis P. G., Thomas F., Villa-Valverde P., Latronico M., Gallo, V, Ragone, R, Musio, B, Todisco, S, Rizzuti, A, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Pascazio, A, Cobas, C, Mari, S, Garino, C, Arlorio, M, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Barison, A, Benevelli, F, Borioni, A, Cagliani, L, Casadei, L, Marincola, F, Colson, K, Consonni, R, Costantino, G, Cremonini, M, Davalli, S, Duarte, I, Guyader, S, Hamon, E, Hegmanns, M, Lamanna, R, Longobardi, F, Mallamace, D, Mammi, S, Markus, M, Menezes, L, Milone, S, Molero-Vilchez, D, Mucci, A, Napoli, C, Rossi, M, Saez-Barajas, E, Savorani, F, Schievano, E, Sciubba, F, Sobolev, A, Takis, P, Thomas, F, Villa-Valverde, P, Latronico, M, Gallo V., Ragone R., Musio B., Todisco S., Rizzuti A., Mastrorilli P., Pontrelli S., Intini N., Scapicchio P., Triggiani M., Pascazio A., Cobas C., Mari S., Garino C., Arlorio M., Acquotti D., Airoldi C., Arnesano F., Assfalg M., Barison A., Benevelli F., Borioni A., Cagliani L. R., Casadei L., Marincola F. C., Colson K., Consonni R., Costantino G., Cremonini M. A., Davalli S., Duarte I., Guyader S., Hamon E., Hegmanns M., Lamanna R., Longobardi F., Mallamace D., Mammi S., Markus M., Menezes L. R. A., Milone S., Molero-Vilchez D., Mucci A., Napoli C., Rossi M. C., Saez-Barajas E., Savorani F., Schievano E., Sciubba F., Sobolev A., Takis P. G., Thomas F., Villa-Valverde P., and Latronico M.

Abstract

Spectroscopic non-targeted methods are gaining ever-growing importance in quality control and authenticity assessment of food products because of their strong potential for identification of specific features of the products by data-driven classifiers. One of the factors hampering the diffusion of spectroscopic non-targeted methods and data-driven classifiers is the lack of harmonized guidelines for their development and validation. In particular, to date, neither conditions to directly compare spectra recorded by different spectrometers nor studies demonstrating the statistical equivalence of the spectra are available. Among the spectroscopic analytical techniques suitable for the development of non-targeted methods, nuclear magnetic resonance (NMR) offers the unique opportunity to generate statistically equivalent signals. In this paper, the feasibility of NMR spectroscopy to generate statistically equivalent NMR signals from a number of different spectrometers was demonstrated for complex mixtures (aqueous extracts of wheat and flour) by organizing an inter-laboratory comparison involving 36 NMR spectrometers. Univariate statistics along with multivariate analysis were exploited to establish unbiased criteria for assessing the statistical equivalence of the NMR signals. The aspects affecting the signal equivalence were investigated, and possible solutions to reduce the extent of the human error were proposed and applied with satisfactory results. This study furnishes the scientific community with an appropriate and easy procedure to validate non-targeted NMR methods and provides error values to be used as a reference for future studies.

Published
2020

7. Characteristics and patterns of care of endometrial cancer before and during COVID-19 pandemic

Author

Bogani, G., Scambia, Giovanni, Cimmino, C., Fanfani, Francesco, Costantini, Barbara, Loverro, Matteo, Ferrandina, Maria Gabriella, Landoni, F., Bazzurini, L., Grassi, T., Vitobello, D., Siesto, G., Perrone, A. M., Zanagnolo, V., De Iaco, P., Multinu, F., Ghezzi, F., Casarin, J., Berretta, R., Capozzi, V. A., Zupi, E., Centini, G., Pellegrino, Antonio Agostino, Corso, S., Stevenazzi, G., Montoli, Stefano, Boschi, A. C., Comerci, Gianluca, Greco, Pierfrancesco, Martinello, R., Sopracordevole, F., Giorda, G., Simoncini, T., Caretto, M., Sartori, E., Ferrari, F., Cianci, A., Sarpietro, G., Matarazzo, M. G., Zullo, F., Bifulco, G., Morelli, Marco, Ferrero, A., Biglia, N., Barra, F., Ferrero, S., Maggiore, U. L. R., Cianci, Stefano, Chiantera, V., Ercoli, Alfredo, Sozzi, G., Martoccia, A., Schettini, Sergio Crescenzo, Orlando, T., Cannone, F. G., Ettore, G., Puppo, A., Borghese, M., Martinelli, C., Muzii, L., Di Donato, V., Driul, L., Restaino, S., Bergamini, A., Candotti, G., Bocciolone, L., Plotti, F., Angioli, R., Mantovani, G., Ceccaroni, Marcello, Cassani, C., Dominoni, M., Giambanco, L., Amodeo, Silvia, Leo, Maria Laura, Thomasset, R., Raimondo, D., Seracchioli, R., Malzoni, M., Gorlero, F., Di Luca, M., Busato, E., Kilzie, S., Dell'Acqua, Antonio, Scarfone, G., Vercellini, P., Petrillo, Marco, Dessole, S., Capobianco, G., Ciavattini, A., Delli Carpini, G., Giannella, L., Mereu, L., Tateo, S., Sorbi, F., Fambrini, M., Cicogna, S., Romano, Federica, Ricci, Giuseppe, Trojano, G., Consonni, R., Cantaluppi, S., Lippolis, A., Tinelli, R., D'Ippolito, G., Aguzzoli, L., Mandato, V. D., Palomba, S., Calandra, D., Rosati, M., Gallo, C., Surico, D., Remorgida, V., Ruscitto, F., Beretta, P., Panici, P. B., Raspagliesi, F., Scambia G. (ORCID:0000-0003-2758-1063), Fanfani F. (ORCID:0000-0003-1991-7284), Costantini B., Loverro M., Ferrandina G. (ORCID:0000-0003-4672-4197), Pellegrino A., Montoli S., Comerci G., Greco P., Morelli M., Cianci S., Ercoli A., Schettini S., Ceccaroni M., Amodeo S., Leo L., Dell'acqua A. (ORCID:0000-0002-8697-3115), Petrillo M., Romano F., Ricci G., Bogani, G., Scambia, Giovanni, Cimmino, C., Fanfani, Francesco, Costantini, Barbara, Loverro, Matteo, Ferrandina, Maria Gabriella, Landoni, F., Bazzurini, L., Grassi, T., Vitobello, D., Siesto, G., Perrone, A. M., Zanagnolo, V., De Iaco, P., Multinu, F., Ghezzi, F., Casarin, J., Berretta, R., Capozzi, V. A., Zupi, E., Centini, G., Pellegrino, Antonio Agostino, Corso, S., Stevenazzi, G., Montoli, Stefano, Boschi, A. C., Comerci, Gianluca, Greco, Pierfrancesco, Martinello, R., Sopracordevole, F., Giorda, G., Simoncini, T., Caretto, M., Sartori, E., Ferrari, F., Cianci, A., Sarpietro, G., Matarazzo, M. G., Zullo, F., Bifulco, G., Morelli, Marco, Ferrero, A., Biglia, N., Barra, F., Ferrero, S., Maggiore, U. L. R., Cianci, Stefano, Chiantera, V., Ercoli, Alfredo, Sozzi, G., Martoccia, A., Schettini, Sergio Crescenzo, Orlando, T., Cannone, F. G., Ettore, G., Puppo, A., Borghese, M., Martinelli, C., Muzii, L., Di Donato, V., Driul, L., Restaino, S., Bergamini, A., Candotti, G., Bocciolone, L., Plotti, F., Angioli, R., Mantovani, G., Ceccaroni, Marcello, Cassani, C., Dominoni, M., Giambanco, L., Amodeo, Silvia, Leo, Maria Laura, Thomasset, R., Raimondo, D., Seracchioli, R., Malzoni, M., Gorlero, F., Di Luca, M., Busato, E., Kilzie, S., Dell'Acqua, Antonio, Scarfone, G., Vercellini, P., Petrillo, Marco, Dessole, S., Capobianco, G., Ciavattini, A., Delli Carpini, G., Giannella, L., Mereu, L., Tateo, S., Sorbi, F., Fambrini, M., Cicogna, S., Romano, Federica, Ricci, Giuseppe, Trojano, G., Consonni, R., Cantaluppi, S., Lippolis, A., Tinelli, R., D'Ippolito, G., Aguzzoli, L., Mandato, V. D., Palomba, S., Calandra, D., Rosati, M., Gallo, C., Surico, D., Remorgida, V., Ruscitto, F., Beretta, P., Panici, P. B., Raspagliesi, F., Scambia G. (ORCID:0000-0003-2758-1063), Fanfani F. (ORCID:0000-0003-1991-7284), Costantini B., Loverro M., Ferrandina G. (ORCID:0000-0003-4672-4197), Pellegrino A., Montoli S., Comerci G., Greco P., Morelli M., Cianci S., Ercoli A., Schettini S., Ceccaroni M., Amodeo S., Leo L., Dell'acqua A. (ORCID:0000-0002-8697-3115), Petrillo M., Romano F., and Ricci G.

Abstract

Objective: Coronavirus disease 2019 (COVID-19) outbreak has correlated with the disruption of screening activities and diagnostic assessments. Endometrial cancer (EC) is one of the most common gynecological malignancies and it is often detected at an early stage, because it frequently produces symptoms. Here, we aim to investigate the impact of COVID-19 outbreak on patterns of presentation and treatment of EC patients. Methods: This is a retrospective study involving 54 centers in Italy. We evaluated patterns of presentation and treatment of EC patients before (period 1: March 1, 2019 to February 29, 2020) and during (period 2: April 1, 2020 to March 31, 2021) the COVID-19 outbreak. Results: Medical records of 5,164 EC patients have been retrieved: 2,718 and 2,446 women treated in period 1 and period 2, respectively. Surgery was the mainstay of treatment in both periods (p=0.356). Nodal assessment was omitted in 689 (27.3%) and 484 (21.2%) patients treated in period 1 and 2, respectively (p<0.001). While, the prevalence of patients undergoing sentinel node mapping (with or without backup lymphadenectomy) has increased during the COVID-19 pandemic (46.7% in period 1 vs. 52.8% in period 2; p<0.001). Overall, 1,280 (50.4%) and 1,021 (44.7%) patients had no adjuvant therapy in period 1 and 2, respectively (p<0.001). Adjuvant therapy use has increased during COVID-19 pandemic (p<0.001). Conclusion: Our data suggest that the COVID-19 pandemic had a significant impact on the characteristics and patterns of care of EC patients. These findings highlight the need to implement healthcare services during the pandemic.

Published
2022

8. Characteristics and patterns of care of endometrial cancer before and during COVID-19 pandemic

Author

Bogani, G, Scambia, G, Cimmino, C, Fanfani, F, Costantini, B, Loverro, M, Ferrandina, G, Landoni, F, Bazzurini, L, Grassi, T, Vitobello, D, Siesto, G, Perrone, A, Zanagnolo, V, De Iaco, P, Multinu, F, Ghezzi, F, Casarin, J, Berretta, R, Capozzi, V, Zupi, E, Centini, G, Pellegrino, A, Corso, S, Stevenazzi, G, Montoli, S, Boschi, A, Comerci, G, Greco, P, Martinello, R, Sopracordevole, F, Giorda, G, Simoncini, T, Caretto, M, Sartori, E, Ferrari, F, Cianci, A, Sarpietro, G, Matarazzo, M, Zullo, F, Bifulco, G, Morelli, M, Ferrero, A, Biglia, N, Barra, F, Ferrero, S, Leone Roberti Maggiore, U, Cianci, S, Chiantera, V, Ercoli, A, Sozzi, G, Martoccia, A, Schettini, S, Orlando, T, Cannone, F, Ettore, G, Puppo, A, Borghese, M, Martinelli, C, Muzii, L, Di Donato, V, Driul, L, Restaino, S, Bergamini, A, Candotti, G, Bocciolone, L, Plotti, F, Angioli, R, Mantovani, G, Ceccaroni, M, Cassani, C, Dominoni, M, Giambanco, L, Amodeo, S, Leo, L, Thomasset, R, Raimondo, D, Seracchioli, R, Malzoni, M, Gorlero, F, Di Luca, M, Busato, E, Kilzie, S, Dell'Acqua, A, Scarfone, G, Vercellini, P, Petrillo, M, Dessole, S, Capobianco, G, Ciavattini, A, Delli Carpini, G, Giannella, L, Mereu, L, Tateo, S, Sorbi, F, Fambrini, M, Cicogna, S, Romano, F, Ricci, G, Trojano, G, Consonni, R, Cantaluppi, S, Lippolis, A, Tinelli, R, D'Ippolito, G, Aguzzoli, L, Mandato, V, Palomba, S, Calandra, D, Rosati, M, Gallo, C, Surico, D, Remorgida, V, Ruscitto, F, Beretta, P, Benedetti Panici, P, Raspagliesi, F, Bogani, Giorgio, Scambia, Giovanni, Cimmino, Chiara, Fanfani, Francesco, Costantini, Barbara, Loverro, Matteo, Ferrandina, Gabriella, Landoni, Fabio, Bazzurini, Luca, Grassi, Tommaso, Vitobello, Domenico, Siesto, Gabriele, Perrone, Anna Myriam, Zanagnolo, Vanna, De Iaco, Pierandrea, Multinu, Francesco, Ghezzi, Fabio, Casarin, Jvan, Berretta, Roberto, Capozzi, Vito A, Zupi, Errico, Centini, Gabriele, Pellegrino, Antonio, Corso, Silvia, Stevenazzi, Guido, Montoli, Serena, Boschi, Anna Chiara, Comerci, Giuseppe, Greco, Pantaleo, Martinello, Ruby, Sopracordevole, Francesco, Giorda, Giorgio, Simoncini, Tommaso, Caretto, Marta, Sartori, Enrico, Ferrari, Federico, Cianci, Antonio, Sarpietro, Giuseppe, Matarazzo, Maria Grazia, Zullo, Fulvio, Bifulco, Giuseppe, Morelli, Michele, Ferrero, Annamaria, Biglia, Nicoletta, Barra, Fabio, Ferrero, Simone, Leone Roberti Maggiore, Umberto, Cianci, Stefano, Chiantera, Vito, Ercoli, Alfredo, Sozzi, Giulio, Martoccia, Angela, Schettini, Sergio, Orlando, Teresa, Cannone, Francesco G, Ettore, Giuseppe, Puppo, Andrea, Borghese, Martina, Martinelli, Canio, Muzii, Ludovico, Di Donato, Violante, Driul, Lorenza, Restaino, Stefano, Bergamini, Alice, Candotti, Giorgio, Bocciolone, Luca, Plotti, Francesco, Angioli, Roberto, Mantovani, Giulia, Ceccaroni, Marcello, Cassani, Chiara, Dominoni, Mattia, Giambanco, Laura, Amodeo, Silvia, Leo, Livio, Thomasset, Raphael, Raimondo, Diego, Seracchioli, Renato, Malzoni, Mario, Gorlero, Franco, Di Luca, Martina, Busato, Enrico, Kilzie, Sami, Dell'Acqua, Andrea, Scarfone, Giovanna, Vercellini, Paolo, Petrillo, Marco, Dessole, Salvatore, Capobianco, Giampiero, Ciavattini, Andrea, Delli Carpini, Giovanni, Giannella, Luca, Mereu, Liliana, Tateo, Saverio, Sorbi, Flavia, Fambrini, Massimiliano, Cicogna, Stefania, Romano, Federico, Ricci, Giuseppe, Trojano, Giuseppe, Consonni, Roberto, Cantaluppi, Simona, Lippolis, Antonio, Tinelli, Raffaele, D'Ippolito, Giovanni, Aguzzoli, Lorenzo, Mandato, Vincenzo D, Palomba, Stefano, Calandra, Davide, Rosati, Maurizio, Gallo, Cinzia, Surico, Daniela, Remorgida, Valentino, Ruscitto, Francesco, Beretta, Paolo, Benedetti Panici, Pierluigi, Raspagliesi, Francesco, Bogani, G, Scambia, G, Cimmino, C, Fanfani, F, Costantini, B, Loverro, M, Ferrandina, G, Landoni, F, Bazzurini, L, Grassi, T, Vitobello, D, Siesto, G, Perrone, A, Zanagnolo, V, De Iaco, P, Multinu, F, Ghezzi, F, Casarin, J, Berretta, R, Capozzi, V, Zupi, E, Centini, G, Pellegrino, A, Corso, S, Stevenazzi, G, Montoli, S, Boschi, A, Comerci, G, Greco, P, Martinello, R, Sopracordevole, F, Giorda, G, Simoncini, T, Caretto, M, Sartori, E, Ferrari, F, Cianci, A, Sarpietro, G, Matarazzo, M, Zullo, F, Bifulco, G, Morelli, M, Ferrero, A, Biglia, N, Barra, F, Ferrero, S, Leone Roberti Maggiore, U, Cianci, S, Chiantera, V, Ercoli, A, Sozzi, G, Martoccia, A, Schettini, S, Orlando, T, Cannone, F, Ettore, G, Puppo, A, Borghese, M, Martinelli, C, Muzii, L, Di Donato, V, Driul, L, Restaino, S, Bergamini, A, Candotti, G, Bocciolone, L, Plotti, F, Angioli, R, Mantovani, G, Ceccaroni, M, Cassani, C, Dominoni, M, Giambanco, L, Amodeo, S, Leo, L, Thomasset, R, Raimondo, D, Seracchioli, R, Malzoni, M, Gorlero, F, Di Luca, M, Busato, E, Kilzie, S, Dell'Acqua, A, Scarfone, G, Vercellini, P, Petrillo, M, Dessole, S, Capobianco, G, Ciavattini, A, Delli Carpini, G, Giannella, L, Mereu, L, Tateo, S, Sorbi, F, Fambrini, M, Cicogna, S, Romano, F, Ricci, G, Trojano, G, Consonni, R, Cantaluppi, S, Lippolis, A, Tinelli, R, D'Ippolito, G, Aguzzoli, L, Mandato, V, Palomba, S, Calandra, D, Rosati, M, Gallo, C, Surico, D, Remorgida, V, Ruscitto, F, Beretta, P, Benedetti Panici, P, Raspagliesi, F, Bogani, Giorgio, Scambia, Giovanni, Cimmino, Chiara, Fanfani, Francesco, Costantini, Barbara, Loverro, Matteo, Ferrandina, Gabriella, Landoni, Fabio, Bazzurini, Luca, Grassi, Tommaso, Vitobello, Domenico, Siesto, Gabriele, Perrone, Anna Myriam, Zanagnolo, Vanna, De Iaco, Pierandrea, Multinu, Francesco, Ghezzi, Fabio, Casarin, Jvan, Berretta, Roberto, Capozzi, Vito A, Zupi, Errico, Centini, Gabriele, Pellegrino, Antonio, Corso, Silvia, Stevenazzi, Guido, Montoli, Serena, Boschi, Anna Chiara, Comerci, Giuseppe, Greco, Pantaleo, Martinello, Ruby, Sopracordevole, Francesco, Giorda, Giorgio, Simoncini, Tommaso, Caretto, Marta, Sartori, Enrico, Ferrari, Federico, Cianci, Antonio, Sarpietro, Giuseppe, Matarazzo, Maria Grazia, Zullo, Fulvio, Bifulco, Giuseppe, Morelli, Michele, Ferrero, Annamaria, Biglia, Nicoletta, Barra, Fabio, Ferrero, Simone, Leone Roberti Maggiore, Umberto, Cianci, Stefano, Chiantera, Vito, Ercoli, Alfredo, Sozzi, Giulio, Martoccia, Angela, Schettini, Sergio, Orlando, Teresa, Cannone, Francesco G, Ettore, Giuseppe, Puppo, Andrea, Borghese, Martina, Martinelli, Canio, Muzii, Ludovico, Di Donato, Violante, Driul, Lorenza, Restaino, Stefano, Bergamini, Alice, Candotti, Giorgio, Bocciolone, Luca, Plotti, Francesco, Angioli, Roberto, Mantovani, Giulia, Ceccaroni, Marcello, Cassani, Chiara, Dominoni, Mattia, Giambanco, Laura, Amodeo, Silvia, Leo, Livio, Thomasset, Raphael, Raimondo, Diego, Seracchioli, Renato, Malzoni, Mario, Gorlero, Franco, Di Luca, Martina, Busato, Enrico, Kilzie, Sami, Dell'Acqua, Andrea, Scarfone, Giovanna, Vercellini, Paolo, Petrillo, Marco, Dessole, Salvatore, Capobianco, Giampiero, Ciavattini, Andrea, Delli Carpini, Giovanni, Giannella, Luca, Mereu, Liliana, Tateo, Saverio, Sorbi, Flavia, Fambrini, Massimiliano, Cicogna, Stefania, Romano, Federico, Ricci, Giuseppe, Trojano, Giuseppe, Consonni, Roberto, Cantaluppi, Simona, Lippolis, Antonio, Tinelli, Raffaele, D'Ippolito, Giovanni, Aguzzoli, Lorenzo, Mandato, Vincenzo D, Palomba, Stefano, Calandra, Davide, Rosati, Maurizio, Gallo, Cinzia, Surico, Daniela, Remorgida, Valentino, Ruscitto, Francesco, Beretta, Paolo, Benedetti Panici, Pierluigi, and Raspagliesi, Francesco

Abstract

Objective: Coronavirus disease 2019 (COVID-19) outbreak has correlated with the disruption of screening activities and diagnostic assessments. Endometrial cancer (EC) is one of the most common gynecological malignancies and it is often detected at an early stage, because it frequently produces symptoms. Here, we aim to investigate the impact of COVID-19 outbreak on patterns of presentation and treatment of EC patients. Methods: This is a retrospective study involving 54 centers in Italy. We evaluated patterns of presentation and treatment of EC patients before (period 1: March 1, 2019 to February 29, 2020) and during (period 2: April 1, 2020 to March 31, 2021) the COVID-19 outbreak. Results: Medical records of 5,164 EC patients have been retrieved: 2,718 and 2,446 women treated in period 1 and period 2, respectively. Surgery was the mainstay of treatment in both periods (p=0.356). Nodal assessment was omitted in 689 (27.3%) and 484 (21.2%) patients treated in period 1 and 2, respectively (p<0.001). While, the prevalence of patients undergoing sentinel node mapping (with or without backup lymphadenectomy) has increased during the COVID-19 pandemic (46.7% in period 1 vs. 52.8% in period 2; p<0.001). Overall, 1,280 (50.4%) and 1,021 (44.7%) patients had no adjuvant therapy in period 1 and 2, respectively (p<0.001). Adjuvant therapy use has increased during COVID-19 pandemic (p<0.001). Conclusion: Our data suggest that the COVID-19 pandemic had a significant impact on the characteristics and patterns of care of EC patients. These findings highlight the need to implement healthcare services during the pandemic.

Published
2022

10. Authenticity of Food

Author

Consonni, R., primary, Astraka, K., additional, Cagliani, L.R., additional, Nenadis, N., additional, Petrakis, E., additional, and Polissiou, M., additional

Published
2016
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14. A community-built calibration system: The case study of quantification of metabolites in grape juice by qNMR spectroscopy

Author

Musio, B, Ragone, R, Todisco, S, Rizzuti, A, Latronico, M, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Di Noia, T, Acquotti, D, Airoldi, C, Assfalg, M, Barge, A, Bateman, L, Benevelli, F, Bertelli, D, Bertocchi, F, Bieliauskas, A, Borioni, A, Caligiani, A, Callone, E, Čamra, A, Cesare Marincola, F, Chalasani, D, Consonni, R, Dambruoso, P, Davalli, S, David, T, Diehl, B, Donarski, J, Gil, A, Gobetto, R, Goldoni, L, Hamon, E, Harwood, J, Kobrlová, A, Longobardi, F, Luisi, R, Mallamace, D, Mammi, S, Martin-Biran, M, Mazzei, P, Mele, A, Milone, S, Molero Vilchez, D, Mulder, R, Napoli, C, Ragno, D, Randazzo, A, Rossi, M, Rotondo, A, Šačkus, A, Sáez Barajas, E, Schievano, E, Sitaram, B, Stevanato, L, Takis, P, Teipel, J, Thomas, F, Torregiani, E, Valensin, D, Veronesi, M, Warren, J, Wist, J, Zailer-Hafer, E, Zuccaccia, C, Gallo, V, Musio, Biagia, Ragone, Rosa, Todisco, Stefano, Rizzuti, Antonino, Latronico, Mario, Mastrorilli, Piero, Pontrelli, Stefania, Intini, Nicola, Scapicchio, Pasquale, Triggiani, Maurizio, Di Noia, Tommaso, Acquotti, Domenico, Airoldi, Cristina, Assfalg, Michael, Barge, Alessandro, Bateman, Lorraine, Benevelli, Francesca, Bertelli, Davide, Bertocchi, Fabio, Bieliauskas, Aurimas, Borioni, Anna, Caligiani, Augusta, Callone, Emanuela, Čamra, Ales, Cesare Marincola, Flaminia, Chalasani, Dinesh, Consonni, Roberto, Dambruoso, Paolo, Davalli, Silvia, David, Taylor, Diehl, Bernd, Donarski, James, Gil, Ana M., Gobetto, Roberto, Goldoni, Luca, Hamon, Erwann, Harwood, John S., Kobrlová, Andrea, Longobardi, Francesco, Luisi, Renzo, Mallamace, Domenico, Mammi, Stefano, Martin-Biran, Magali, Mazzei, Pierluigi, Mele, Andrea, Milone, Salvatore, Molero Vilchez, Dolores, Mulder, Roger J., Napoli, Claudia, Ragno, Daniele, Randazzo, Antonio, Rossi, Maria Cecilia, Rotondo, Archimede, Šačkus, Algirdas, Sáez Barajas, Elena, Schievano, Elisabetta, Sitaram, Bhavaraju, Stevanato, Livio, Takis, Panteleimon G., Teipel, Jan, Thomas, Freddy, Torregiani, Elisabetta, Valensin, Daniela, Veronesi, Marina, Warren, John, Wist, Julien, Zailer-Hafer, Elina, Zuccaccia, Cristiano, Gallo, Vito, Musio, B, Ragone, R, Todisco, S, Rizzuti, A, Latronico, M, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Di Noia, T, Acquotti, D, Airoldi, C, Assfalg, M, Barge, A, Bateman, L, Benevelli, F, Bertelli, D, Bertocchi, F, Bieliauskas, A, Borioni, A, Caligiani, A, Callone, E, Čamra, A, Cesare Marincola, F, Chalasani, D, Consonni, R, Dambruoso, P, Davalli, S, David, T, Diehl, B, Donarski, J, Gil, A, Gobetto, R, Goldoni, L, Hamon, E, Harwood, J, Kobrlová, A, Longobardi, F, Luisi, R, Mallamace, D, Mammi, S, Martin-Biran, M, Mazzei, P, Mele, A, Milone, S, Molero Vilchez, D, Mulder, R, Napoli, C, Ragno, D, Randazzo, A, Rossi, M, Rotondo, A, Šačkus, A, Sáez Barajas, E, Schievano, E, Sitaram, B, Stevanato, L, Takis, P, Teipel, J, Thomas, F, Torregiani, E, Valensin, D, Veronesi, M, Warren, J, Wist, J, Zailer-Hafer, E, Zuccaccia, C, Gallo, V, Musio, Biagia, Ragone, Rosa, Todisco, Stefano, Rizzuti, Antonino, Latronico, Mario, Mastrorilli, Piero, Pontrelli, Stefania, Intini, Nicola, Scapicchio, Pasquale, Triggiani, Maurizio, Di Noia, Tommaso, Acquotti, Domenico, Airoldi, Cristina, Assfalg, Michael, Barge, Alessandro, Bateman, Lorraine, Benevelli, Francesca, Bertelli, Davide, Bertocchi, Fabio, Bieliauskas, Aurimas, Borioni, Anna, Caligiani, Augusta, Callone, Emanuela, Čamra, Ales, Cesare Marincola, Flaminia, Chalasani, Dinesh, Consonni, Roberto, Dambruoso, Paolo, Davalli, Silvia, David, Taylor, Diehl, Bernd, Donarski, James, Gil, Ana M., Gobetto, Roberto, Goldoni, Luca, Hamon, Erwann, Harwood, John S., Kobrlová, Andrea, Longobardi, Francesco, Luisi, Renzo, Mallamace, Domenico, Mammi, Stefano, Martin-Biran, Magali, Mazzei, Pierluigi, Mele, Andrea, Milone, Salvatore, Molero Vilchez, Dolores, Mulder, Roger J., Napoli, Claudia, Ragno, Daniele, Randazzo, Antonio, Rossi, Maria Cecilia, Rotondo, Archimede, Šačkus, Algirdas, Sáez Barajas, Elena, Schievano, Elisabetta, Sitaram, Bhavaraju, Stevanato, Livio, Takis, Panteleimon G., Teipel, Jan, Thomas, Freddy, Torregiani, Elisabetta, Valensin, Daniela, Veronesi, Marina, Warren, John, Wist, Julien, Zailer-Hafer, Elina, Zuccaccia, Cristiano, and Gallo, Vito

Abstract

Nuclear Magnetic Resonance (NMR) is an analytical technique extensively used in almost every chemical laboratory for structural identification. This technique provides statistically equivalent signals in spite of using spectrometer with different hardware features and is successfully used for the traceability and quantification of analytes in food samples. Nevertheless, to date only a few internationally agreed guidelines have been reported on the use of NMR for quantitative analysis. The main goal of the present study is to provide a methodological pipeline to assess the reproducibility of NMR data produced for a given matrix by spectrometers from different manufacturers, with different magnetic field strengths, age and hardware configurations. The results have been analyzed through a sequence of chemometric tests to generate a community-built calibration system which was used to verify the performance of the spectrometers and the reproducibility of the predicted sample concentrations.

Published
2020

17. NMR Spectroscopy

Author

Cagliani LR, Scano P, and Consonni R

Subjects
NMR
Abstract

Chapter 6 in: Food analysis and properties series: spectroscopic methods in food analysis

Published
2018

18. Plant development and crop productivity for sustainable agriculture

Author

Mascheretti I., Locatelli F., Cusano E., Consonni R., and Mattana M.

Subjects
CELL COLTURE, FLAX, NMR
Abstract

Flax is one of the oldest crop being widely grown for its oil, fiber content and food nutrients such as high quality omega-3 fatty acids (?-linolenic acid), proteins, lignin and phenolic acids (lignans). Besides industrial and nutritional importance, flaxseeds exhibit numerous pharmacological activities due to the presence of biologically active components (lignans) that show antioxidant, cytotoxic, antifungal, antiviral and phytoestrogenic properties. Furthermore, numerous studies have demonstrated the role of these compounds in reducing the risk of certain types of cancers especially the breast, colon and prostate cancers. Therefore, due to the growing need of these medically and nutritionally important metabolites, it is crucial to find sustainable and eco-friendly alternative system for their production on large scale. In vitro plant cell cultures represents a promising source for rapid and enhanced production of desired plant metabolites. Indeed, the production of these metabolites can be enhanced by the use of specific elicitors that in small quantities promote the biosynthesis of secondary metabolites. Flax comprises over 180 species taxonomically divided into 2 clades. The species belonging to the first clade are characterized by the accumulation of podophyllotoxin type lignans, which show cytotoxic activity and are successfully used as anticancer compounds. The species belonging to the second clade accumulate the justicidin B and secoisolaricilresinol (SECO) as major lignans, which show an anti-inflammatory, anti-viral and cytotoxic activity. The aim of this research project was to exploit different flax species for improving lignans production in vitro cell cultures. To this purpose, we analyzed the total phenol content, the antioxidant capacity and the metabolite profile of the cell extracts in order to identify flax species that produce the highest level of metabolites. We have also evaluated the effect of the elicitor methyl-jasmonate on metabolites production, and we are now planning to apply a metabolic engineering approach to improve the flax bioactive compound production.

Published
2018

21. NMR metabolomics in food authentication

Author

Consonni R and Cagliani LR

Subjects
food authentication, metabolomics, nmr
Abstract

In the last years there was an increasing interest on NMR spectroscopy, whose application experienced an exponential growth in several research fields, particularly in food analysis. NMR represents the elective technique for the structure elucidation of molecules and it is particularly suited to analyze complex mixtures, like foods. Its use in analytical routines, characterized by rapid and reproducible measurements, would provide the identification of a wide range of chemical compounds in the mixture under investigation simultaneously, revealing potential markers or disclosing sophisticated frauds. The great economic values of high quality or guaranteed food products demands highly refined characterization tools to protect consumers and the productions of valuable foods. This scenario suggests metabolomics as a privileged approach for the modern analytical studies in the next decades.

Published
2017

22. NMR Based Plants and Food Metabolomics

Author

Consonni R

Subjects
metabpolomics, NMR
Abstract

In these last decades, the "omics" era allowed to face new challenges, involving new analytical approaches. The point of view has been changed and a broader vision of the biological processes is forced for a proper understanding. This broadened the data size as well as the number of molecules to be monitored and in this sense "metabolomics" constitute the new approach. In these last years, several studies focusing the metabolite characterization of both foods and plants appeared in the literature. Particularly, two analytical approaches emerged for their pivotal role: mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). This latter technique, largely adopted in the past years for its peculiarity in the structural characterization of single molecules/biomolecules, experienced a new relaunch in the investigation of mixtures, especially food and plant extracts1-3. The NMR approach takes the advantage of analysing the metabolite content with a single experiment in a non-destructive way. Moreover, very simple or none sample preparation is required, thus making this technique very low demanding in terms of data acquisition and sample preparation. In plants, several studies are present concerning the metabolic investigations by NMR followed by the multivariate statistical analysis of data, allowing easier data interpretation, marker and/or new molecules discovery. Comparison of metabolite profile of treated or non-treated samples is also facilitated. In the present communication, the potentiality of the NMR approach will be presented with the aid of different practical examples. In addition, the basic elements of the NMR technique will be provided, also in combination with the use of chemometrics.

Published
2017

24. NMR spectroscopy: a valid tool in targeting bioactive molecules

Author

Consonni R and Cagliani L.R.

Subjects
nutraceuticals, bioactive compounds, metabolomics, NMR
Abstract

NMR spectroscopy is a well establish analytical method in structural characterization of macromolecules. In these last years, NMR has been accepted as a preferential analytical method for mixture investigations, playing an important role in the "omics" technologies. Food matrices, as well as plant extracts and by-products, have been investigated, not only in metabolite fingerprinting approaches but also in sourcing for bioactive compounds1-5. The NMR approach takes the advantage of analyzing the metabolite content with a single experiment in a non-destructive way. Moreover, very simple or none sample preparation is required, thus making this technique very low demanding in terms of data acquisition and sample preparation. In plants, several studies are present concerning the metabolic investigations by NMR followed by the multivariate statistical analysis of data, allowing easier data interpretation, marker and/or new molecules discovery. Comparison of metabolite profile of treated or non-treated samples is also facilitated. In the present communication, the potentiality of the NMR approach will be presented with the aid of different practical examples.

Published
2017

26. Caratterizzazione della qualità e dell'origine dello zafferano

Author

Cagliani L.R., Chessa M., Culeddu N., Lalou S., Naziri E., Ordoudi S.A., Petrakis E.A., Polissiou M.G., Tsimidou M.Z., and Consonni R.

Subjects
Qualità, Zafferano
Abstract

In questo lavoro vengono analizzati diversi aspetti qualitativi dello zafferano (Crocus sativus L.) mediante l'impiego di spettroscopia 1H NMR ed analisi statistica multivariata. In particolare è stato possibile ottenere la differenziazione del prodotto italiano DOP da quello commerciale, l'identificazione di eventuali bio-adulteranti e la definizione del periodo di stoccaggio durante il quale il prodotto può essere considerato ancora fresco. Parte del lavoro qui presentato è stato realizzato nell'ambito del progetto COST ACTION FA1101 "SaffronOmics".

Published
2016

27. Insect pollination effects on antioxidant content of Perilla frutescens seeds analyzed by NMR spectroscopy

Author

Ferrazzi P., Chakir A., Cusano E., Mattana M., Romane A., Vercelli M., and Consonni R.

Subjects
fungi, food and beverages, Perilla, Antioxidant, NMR, Antioxidants
Abstract

The importance of perilla frutescens as a species rich in medicinal properties and with a high antioxidant content is increasingly know. The use of this asian plant is spreading worldwide. Our previous studies regarding visits by honey bees and other insect pollinators on Perilla aimed to explore the possible effects on the pollinator action on the content of active ingredients of seeds from this species . The positive effects of pollinating insects and in particular the honey bee, are well known in crop plants such as fruit, oilseed and fodder, but have not been studied in medicinal plants. Pollination tests were conducted in september (2013) by isolating plants of Perilla with mesh cages before blooming and considering another plants left open to insect pollination. At maturity, seeds were collected counted and weighed. In (2015) additional trials were carried out to assess possible differences in antioxidant content of open pollination plants. The difference in seeds number and weight between open pollinated flowers recorded using a 600 MHz spectrometer showed the presence of rosmarinic acid, luteolin and apigenin, in line with previous studies, while their content was increased in plants with open insect pollination. In particular seeds from open (2013) compared to caged plants indicated the presence of glucosylated flavonoid, most likely due the proteolitic activity of glucosidic enzymes. Seeds from open-pollinated (2015) plants showed a content of luteolin and epigenin that was increased by 3-fold compared to self-polinated (2013) plants. As he honey bee is the main pollinator of Perilla, its important role in increasing the presence of antioxidants is becoming more evident.

Published
2016

28. Different spectroscopic approaches for the analysis of secondary metabolites derived from different tissues of Crocus sativus L. flower cultivated in Italy

Author

Cusano E., Consonni R., Petrakis E.A., Astraka K., Cagliani L.R., and Polissiou M.G.

Subjects
Saffron, FT-IR, GS-MS, food and beverages, HPLC-DAD, Raman, NMR
Abstract

In this study, several spectroscopic and chromatographic techniques were employed to investigate the metabolic profile of different tissues of Crocus sativus L. flower cultivated in Italy. The volatile profiling of stigmas was characterized by GC-MS [1] for samples with the same genetic and geographical origin, revealing some differences associated with different drying processes applied. LC-DAD, NMR [2], FT-IR [3], and Raman were used to explore quality parameters of saffron while other C. sativus flower parts (i.e. tepals and stamens) were also investigated. The metabolic profiling of saffron samples with different genetic origin but produced in the same region was also examined. C. sativus stamens, a possible bioadulterant, were studied to investigate potential marker compounds useful for their identification. The results obtained highlight some new aspects of genuine saffron sold in Italy.

Published
2016

30. New insight in the saffron quality characterization: the NMR based approach

Author

Cagliani Laura Ruth, Lalou sofia, Naziri Eleni, Ordoudi Stella A., Petrakis Eleftherios A., Polissiou Moschos G., Tsimidou Maria Z., and Consonni R.

Subjects
Saffron, quality, NMR
Abstract

In the last years several efforts have been driven to food authentication and new analytical techniques have been applied in the view of a deeper characterization. The growth of a new discipline, called "Food-omics" evidenced the need of a larger exploration of different aspects related to food. From the chemical point of view, a food is considered a complex matrix containing different chemical compounds widely ranging in concentration and in chemical properties. In this context NMR (Nuclear Magnetic Resonance) spectroscopy represents a valid and powerful technique allowing the analysis of different classes of chemical compounds with high -medium abundance, within a single experiment, using a limited experimental time and no chemical treatment of sample. In this work, the NMR analysis is presented to assess the quality of saffron, nowadays defined by the specifications and methods described in the ISO 3632 trade standard. A particular emphasis will be dedicated to the results achieved during the STSMs approved by COST Action FA 1101 "SaffronOmics". The use of multivariate statistical analysis on 1H NMR data obtained from DMSO extracts of saffron, allowed to evaluate different quality aspects: the geographical discrimination between Greek and Iranian saffron of the same harvest, the definition of the storage period during which saffron can be considered yet fresh, irrespectively from the origin, and the identification of possible bio-adulterants such as C. sativus stamens, safflower, turmeric, and gardenia. These findings highlight the potentiality of NMR spectroscopy in quality assessment of the most expensive spice worldwide, protecting both producers and consumers.

Published
2015

31. Production of bioactive secondary metabolites from flax and hemp through in vitro technology

Author

Gabotti D., Locatelli F., Cusano E., Consonni R., Baldoni E., Adì A., and Mattana M.

Subjects
secondary metabolites, flax, food and beverages, hemp, NMR
Abstract

The aim of this work was to study the ability of flax and hemp Liquid Cell Cultures (LCCs) to produce and accumulate secondary metabolites of interest for industrial/pharmaceutical purposes. At first, seven cultivars of hemp and flax were tested with a combined approach of biochemistry, molecular biology and 1H-NMR profiling to select the most promising cultivar of both species. qRT-PCR of two key genes of phenylpropanoid pathway (PAL and 4CL) and their relative specific enzymatic activity have been determined, as well as the metabolic profile of leaves extracts. On the basis of these results two cultivars, 'Valoal' for flax and 'Futura' for hemp, have been chosen to set up the cell cultures. The obtained LCCs have been characterized for total phenolics and flavonoids content, free radical scavenging activity (DPPH assay) and metabolic profiles- (1H-NMR). According to the class of metabolites identified the relative biosynthetic pathway has been examined by mean of key enzymatic activities and relative mRNA expression (qRT-PCR).

Published
2015

32. NMR metabolomics approach in saffron analysis

Author

Cagliani L.R., Chessa, Culeddu, Lalou S., Naziri E., Ordoudi S.A., Petrakis E.A., Polissiou M.G., Tarantilis, P.A., Tsimidou M.Z., and Consonni R

Subjects
quality, saffron, authentication, metabolomics, NMR
Abstract

not required

Published
2015

33. Attitudes Toward Randomized, Controlled Trials in Surgery: Opinions of Gynecologists of the Gruppo Italiano di Studio sull'Endometriosi

Author

Aguzzoli, L., Alio, L., Aretini, M., Barletta, F., Beretta, P., Bertulessi, C., Bianchi, A., Bracco, G. L., Busacca, M., Cappiello, F., Casa, ., Cela, V., Ciavattini, A., Congiu, M. A., Consonni, R., Dal, Pozzo, Del Frate, G., Cristiano, A. D., Di Dionisio, A., Dolcetta, G., Filliolini, C., Genazzani, A. R., Gerli, S., Giambanco, V., La Greca, M., La Grotta, F., Litta, PIETRO SALVATORE, Mais, V., Mariani, L., Martinello, R., Martulli, B., Mazzini, M., Meroni, N., Micalef, S., Montanino, O. M., Muriana, A., Pasini, A., Pieroni, A., Pittino, M., Porpora, M. G., Puglisi, A. S., Saccucci, P., Santuz, M., Scollo, P., Sesti, F., Stola, E., Tinelli, F., Vicino, M., Volpi, E., Zupi, E., and Parazinni, F.

Subjects
endometriosis, treatment planning, medicine.medical_specialty, Alternative medicine, randomization, surgical technique, medical research, law.invention, gynecologic surgery, Randomized controlled trial, law, medicine, controlled study, human, normal human, skill, Random allocation, business.industry, article, Obstetrics and Gynecology, financial management, Surgical procedures, Surgery, priority journal, attitude, business
Abstract

In order to assess barriers and the attitudes of gynecologists toward randomized controlled trials (RCT) in surgery for endometriosis, we identified 62 gynecologists affiliated with the Gruppo Italiano di Studio sull'Endometriosi and sent them a questionnaire. A total of 52 (83.9%) gynecologists completed and sent it back to the coordinating center. Most gynecologists strongly agreed or agreed that RCTs should be the study design of choice for evaluating surgical procedures, but 66% also strongly agree/agree that RCTs are best suited to nonsurgical setting. With regard to the feasibility of RCT in surgery, there was a high level (>50%) of agreement with the statement: RCTs are insufficiently funded; the surgical community gives to RCTs too low priority; patients are unlikely to accept random allocation. A total of 43% agree or strongly agree that there is often consensus on new procedures without the need for RCTs, but 49% disagree or strongly disagree. Considering the statements on methodological problem...

Published
2004
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35. Performance of a novel sieving matrix of poly(vinyl alcohol)/acrylamide copolymer in electrophoretic separations of high molecular weight proteins from red cell membrane

Author

Matte, A, Sola, L, Chiari, M, Tomelleri, C, Consonni, R, Turrini, Francesco Michelangelo, and Franceschi, De

Subjects
Acrylamide, Erythrocyte Membrane, Acrylic Resins, Membrane Proteins, two-dimensional electrophoresis, red cells, Molecular Weight, proteomics, Polyvinyl Alcohol, Humans, high molecular weight proteins, Electrophoresis, Polyacrylamide Gel, Porosity
Abstract

The analysis of high molecular weight (HMW) proteins from complex mixtures is still a challenge in proteomics. This work introduces a novel hydrogel obtained by the copolymerization of an allyl-PVA derivative with acrylamide and bisacrylamide and applies this matrix to the electrophoretic separation of HMW proteins. By inducing gelation of polyacrylamide in the presence of variable amounts of allyl-PVA, it is possible to control and vary the average gel porosity. This gel is easy to produce and handle and offers the advantage of being highly mechanically resistant and macroporous. The new matrix was tested in mono-dimensional separations of complex protein mixtures extracted from red cell membranes with different detergents. The improved performance of this macroporous matrix allowed to identify new proteins by MS and immunoblot analysis using specific antibodies. In particular, the resolution of proteins ranging in size between 97 and 279 kDa was greatly improved here compared to standard polyacrylamide gels, suggesting that this matrix can be a useful tool in routine analysis of HMW proteins in cell biology.

Published
2014
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38. New 'clickable' polymeric coating for glycan microarrays

Author

Zilio, C, Bernardi, A, Palmioli, A, Salina, M, Tagliabue, G, Buscaglia, M, Consonni, R, Chiari, M, Zilio, Caterina, Bernardi, Anna, Palmioli, Alessandro, Salina, Matteo, Tagliabue, Giovanni, Buscaglia, Marco, Consonni, Roberto, Chiari, Marcella, Zilio, C, Bernardi, A, Palmioli, A, Salina, M, Tagliabue, G, Buscaglia, M, Consonni, R, Chiari, M, Zilio, Caterina, Bernardi, Anna, Palmioli, Alessandro, Salina, Matteo, Tagliabue, Giovanni, Buscaglia, Marco, Consonni, Roberto, and Chiari, Marcella

Abstract

The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. In this study, a novel slide is reported for the fabrication of glycan arrays that combines the higher sensitivity of a layered Si-SiO2 with a novel approach to form a polymeric coating easily modifiable by subsequent click reaction. The alkyne-containing copolymer, adsorbed from an aqueous solution, produces a coating by a single step procedure and serves as a soft, tridimensional support for the oriented immobilization of carbohydrates via azide/alkyne Cu(I) catalyzed "click" reaction. The equilibrium and kinetics parameters of the interaction of Concanavalin A with eight synthetic glycans were determined using fluorescence microarray and Reflective Phantom Interface (RPI), a recently proposed optical label-free detection approach. The enhancement of fluorescence provided by the Si-SiO2 slides enabled to extend the limit of detection at lower surface densities of lectins, in turn enabling the study of the interaction for a wide range of glycans surface density. Equilibrium dissociation constants of a few nM were extracted for multivalent glycan-lectin binding, mimicking the conditions of biological membranes, whereas hundreds of nM were observed at the lower glycan surface densities.

Published
2015

39. Performance Assessment in Fingerprinting and Multi Component Quantitative NMR Analyses

Author

Gallo, V, Intini, N, Mastrorilli, P, Latronico, M, Scapicchio, P, Triggiani, M, Bevilacqua, V, Fanizzi, P, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Benevelli, F, Bertelli, D, Cagliani, L, Casadei, L, Cesare Marincola, F, Colafemmina, G, Consonni, R, Cosentino, C, Davalli, S, De Pascali, S, D'Aiuto, V, Faccini, A, Gobetto, R, Lamanna, R, Liguori, F, Longobardi, F, Mallamace, D, Mazzei, P, Menegazzo, I, Milone, S, Mucci, A, Napoli, C, Pertinhez, T, Rizzuti, A, Rocchigiani, L, Schievano, E, Sciubba, F, Sobolev, A, Tenori, L, Valerio, M, Gallo, V, Intini, N, Mastrorilli, P, Latronico, M, Scapicchio, P, Triggiani, M, Bevilacqua, V, Fanizzi, P, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Benevelli, F, Bertelli, D, Cagliani, L, Casadei, L, Cesare Marincola, F, Colafemmina, G, Consonni, R, Cosentino, C, Davalli, S, De Pascali, S, D'Aiuto, V, Faccini, A, Gobetto, R, Lamanna, R, Liguori, F, Longobardi, F, Mallamace, D, Mazzei, P, Menegazzo, I, Milone, S, Mucci, A, Napoli, C, Pertinhez, T, Rizzuti, A, Rocchigiani, L, Schievano, E, Sciubba, F, Sobolev, A, Tenori, L, and Valerio, M

Abstract

An interlaboratory comparison (ILC) was organized with the aim to set up quality control indicators suitable for multicomponent quantitative analysis by nuclear magnetic resonance (NMR) spectroscopy. A total of 36 NMR data sets (corresponding to 1260 NMR spectra) were produced by 30 participants using 34 NMR spectrometers. The calibration line method was chosen for the quantification of a five-component model mixture. Results show that quantitative NMR is a robust quantification tool and that 26 out of 36 data sets resulted in statistically equivalent calibration lines for all considered NMR signals. The performance of each laboratory was assessed by means of a new performance index (named Qp-score) which is related to the difference between the experimental and the consensus values of the slope of the calibration lines. Laboratories endowed with a Qp-score falling within the suitable acceptability range are qualified to produce NMR spectra that can be considered statistically equivalent in terms of relative intensities of the signals. In addition, the specific response of nuclei to the experimental excitation/relaxation conditions was addressed by means of the parameter named NR. NR is related to the difference between the theoretical and the consensus slopes of the calibration lines and is specific for each signal produced by a well-defined set of acquisition parameters. (Graph Presented).

Published
2015

44. NMR e zuccheri del miele: caratterizzazione botanica e differenziazione geografica

Author

Cagliani L.R., Consonni R., and Cogliati C.

Abstract

Negli ultimi anni l'attenzione dei consumatori e dei produttori è sempre più rivolta alla ricerca dell'autenticità degli alimenti. Nel caso del miele, alimento largamente diffuso e apprezzato in tutto il mondo per le sue caratteristiche nutritive, antibatteriche e medicinali, l'autenticità è correlata all' origine botanica, all' origine geografica ed alla composizione (assenza di aggiunte illecite di zuccheri o sciroppi). Attualmente la definizione dell'origine geografica e botanica del miele è valutata mediante l'analisi melissopalinologica, che presenta però dei limiti [1]. L'NMR ha già dimostrato la sua potenzialità nel definire l'autenticità di diverse matrici alimentari [2]. In questo lavoro vengono presentati studi combinati di NMR e chemiometria per la caratterizzazione botanica e per la differenziazione dell'origine geografica di mieli sulla base unicamente del contenuto di 19 zuccheri identificati. In particolare è stato possibile, all'interno della stessa specie botanica, distinguere mieli provenienti da diversi stati e mieli provenienti da regioni italiane limitrofe. Referenze [1] P. C. Molan Bee World 79, 59-68 (1998) [2] R. Consonni, and L. R. Cagliani ACS Sym. Ser. 1081, Chapter 2, 13-26 (2011)

Published
2012

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