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3 results on '"Copper-zinc alloy nanoparticles"'

1. Evaluation of in vitro cytotoxicity and genotoxicity of copper–zinc alloy nanoparticles in human lung epithelial cells.

Author

Kumbıçak, Ümit, Çavaş, Tolga, Çinkılıç, Nilüfer, Kumbıçak, Zübeyde, Vatan, Özgür, and Yılmaz, Dilek

Subjects
*GENETIC toxicology, *EPITHELIAL cells, *PHYSIOLOGICAL effects of nanoparticles, *COPPER-zinc alloys, *TRANSMISSION electron microscopy, *ZETA potential, *NUCLEOLUS, *IN vitro studies
Abstract

In the present study, in vitro cytotoxic and genotoxic effect of copper–zinc alloy nanoparticles (Cu–Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ROS Elisa assays. Genotoxic effects were evaluated using micronucleus, comet and γ-H2AX foci assays. Cu–Zn ANPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Characterization of Cu–Zn ANPs showed an average size of 200 nm and zeta potential of −22 mV. TEM analyses further revealed the intracellular localization of Cu–Zn ANPs in cytoplasm within 24 h. Analysis of micronucleus, comet and γ-H2AX foci counts showed that exposure to Cu–Zn ANPs significantly induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Our results further indicated that exposure to Cu–Zn ANPs significantly induced intracellular ROS formation. Evaluation of M30:M65 ratios suggested that cell death was predominantly due to necrosis. [ABSTRACT FROM AUTHOR]

Published
2014
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2. Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells

Author

Zübeyde Kumbıçak, Tolga Çavaş, Nilufer Cinkilic, Dilek Yilmaz, Ümit Kumbıçak, Ozgur Vatan, Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Çavaş, Tolga, Çinkılıç, Nilüfer, Vatan, Özgür, Yılmaz, Dilek, AAH-3508-2021, O-7508-2015, and AAH-5296-2021

Subjects
Cell viability, Cellular distribution, Cytotoxicity, Enzyme linked immunosorbent assay, Metal Nanoparticles, Apoptosis, IC50, medicine.disease_cause, Toxicology, Epithelium cell, CUO, Nickel, Nanotoxicology, Lung, Chemistry, Zinc Oxide Nanoparticle, Antibacterial Activity, Zinc Nitrate, General Medicine, Particle size, Double stranded DNA break, Zinc, Intracellular, Copper-zinc alloy nanoparticles, Lung alveolus epithelium, Human, Programmed cell death, Histone H2AX, DNA damage, Carcinogenicity Tests, Enzyme-Linked Immunosorbent Assay, In Vitro Techniques, Food science & technology, Article, Metal-oxide nanoparticles, Zno nanoparticles, Necrosis, Microscopy, Electron, Transmission, BEAS-2B cells, Chromosome damage, medicine, Humans, Clonogenic assay, Nanomaterials, Drug effects, Toxicity, DNA-damage, Mutagenicity Tests, Zinc oxide nanoparticle, In vitro study, Epithelial Cells, Zeta potential, Carcinogen testing, Molecular biology, Metabolism, Human cell, Micronucleus, Oxidative stress, Release, Genotoxicity, Copper nanoparticle, Cytology, Reactive Oxygen Species, Reactive oxygen metabolite, Controlled study, Metal nanoparticle, Mutagen testing, Transmission electron microscopy, Copper, Food Science
Abstract

In the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ROS Elisa assays. Genotoxic effects were evaluated using micronucleus, comet and gamma-H2AX foci assays. Cu-Zn ANPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Characterization of Cu-Zn ANPs showed an average size of 200 nm and zeta potential of -22 mV. TEM analyses further revealed the intracellular localization of Cu-Zn ANPs in cytoplasm within 24 h. Analysis of micronucleus, comet and gamma-H2AX foci counts showed that exposure to Cu-Zn ANPs significantly induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Our results further indicated that exposure to Cu-Zn ANPs significantly induced intracellular ROS formation. Evaluation of M30:M65 ratios suggested that cell death was predominantly due to necrosis.

Published
2014

3. Bakır-çinko alaşım nanopartiküllerinin in vitro sitotoksik ve genotoksik etkilerinin araştırılması

Author

Kumbiçak, Ümit, Çavaş, Tolga, Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı., and Biyoloji Ana Bilim Dalı

Subjects
Bakır-çinko alaşım nanopartikülleri, BEAS-2B cells, Cytotoxicity, BEAS- 2B hücreler, Genotoksisite, Nanotoksikoloji, Sitotoksisite, Genotoxicity, Nanotoxicology, Biology, Biyoloji, Copper-zinc alloy nanoparticles
Abstract

Bu çalışmada Cu-Zn alaşım nanopartiküllerin BEAS-2B insan akciğer epitel hücreleri üzerindeki sitotoksik ve genotoksik etkileri araştırılmıştır. Sitotoksik etkilerin belirlenmesi için XTT ve klonojenik test kullanılmıştır. Genotoksik etkiler ise mikronüklus, komet ve ?-H2AX fokus testleri kullanılarak belirlenmiştir. Ayrıca, apoptotik ve nekrotik etkiler ile ROS oluşumunun belirlenmesi için M30, M65 ve ROS testleri uygulanmıştır. Deneylerden önce, kullanılan nanopartikül süspansiyonunun karakterizasyonu için zeta potansiyeli, DLS ve TEM analizleri kullanılarak gerçekleştirilmiştir. XTT ve Klonojenik testler için BEAS-2B hücreleri 24 saat süresince 0,1 ile 100 µg/ml değişen dozlarda Cu-Zn alaşım nanopartiküllerine maruz bırakılmışlardır. Elde edilen IC50 değerine (4,66 µg/ml) dayanarak genotoksisite testlerinde kullanılacak dozlar (0,1-3,2 µg/ml) belirlenmiştir. Çalışılan süspansiyonun karakterizasyonu sonucunda zeta potansiyeli -22 mV ve ortalama partikül büyüklüğü de 200 nm olarak belirlenmiştir. TEM analizlerinde ayrıca Cu-Zn alaşım nanopartiküllerinin sitoplazma içerisinde lokalize olduğu belirlenmiştir. Mikronükleus, komet ve ?-H2AX fokus testlerinin sonucunda Cu-Zn alaşım nanopartiküllerinin kromozomal hasarlar yanında tek ve çift iplik DNA kırıklarına yol açtığı belirlenmiştir. Çalışmamızda belirlenen M30:M65 oranları ise hücre ölümünün ağırlıklı olarak nekroz yoluyla gerçekleştiğini göstermiştir. Ayrıca, elde ettiğimiz bulgular gözlenen sitotoksik ve genotoksik etkilerden hücre içi ROS oluşumunun sorumlu olabileceğini göstermiştir. In the present study, cytotoxic and genotoxic effect of copper-zinc (Cu-Zn) alloy nanoparticles on BEAS-2B human epithelial cells were evaluated. XTT test and clonogenic assay were used to determine cytotoxic effects. Genotoxic effects were evaluated using micronucleus, comet and ?-H2AX foci assays. Furthermore, M30, M65 and ROS assays were applied to evaluate apoptotic and necrotic effects as well as intracellular reactive oxygen species formation, respectively. Before the experiments, characterization of the nanoparticle suspension was carried out using zeta potential, DLS and TEM analyses. For XTT and clonogenic assays, BEAS-2B cells were exposed the different concentrations (0,1-100 µg/ml) of Cu-Zn alloy nanoparticles. Based on the determined IC50 value (4,66 µg/ml), concentrations to be used in genotoxicity experiments (0,1-3,2 µg/ml) were determined. Characterization of the studied suspension showed that the zeta potential was ?22 mV and the average size was 200 nm. TEM analyses further revealed the intracellular localization of Cu-Zn alloy nanoparticles in cell cytoplasm. Analysis of micronucleus, comet and ?-H2AX foci frequencies showed that exposure to Cu-Zn alloy nanoparticles induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Evaluation of M30:M65 ratios suggest that cell death was predominantly due to necrosis. Our results further indicated that increased intracellular ROS formation could be responsible from the observed cytotoxic and genotoxic effects. 128

Published
2013

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