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1. Current knowledge and practice of Candida auris screening in France: A nationwide survey from the French Society of Medical Mycology (SFMM)

Author

Guitard, J., Bellanger, A.P., Dorin, J., Cassaing, S., Capitaine, A., Gabriel, F., Nicolas, M., Coron, N., Penn, P., Moniot, M., Quinio, D., Ranque, S., Sasso, M., Lepape, P., Dannaoui, E., Brun, S., Lacroix, C., Cornu, M., Debourgogne, A., Durieux, M.F., Laurent, G., Bru, V., Bourgeois, N., Brunet, K., Chouaki, T., Huguenin, A., Hasseine, L., Maubon, D., Gangneux, J.P., Desbois-Nogard, N., Houze, S., Dalle, F., Bougnoux, M.E., Alanio, A., Costa, D., Botterel, F., and Hennequin, C.

Published
2024
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3. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)

Author

Brieu, N., Durand, C., Bertei, D., Bouchara, J.P., Pihet, M., Bland, S., Bru, J.P., Pulik, M., Le Turdu, F., Lefrand C, H., Ferrand, M., Larrouy, M., Millon, L., Delhaes, L., Imbert, S., Accoceberry, I., Bachelier, M.N., Nevez, G., Quinio, D., Le Coustumier, A., Carmagnol, F., Rivière, B., Boex, P., Podac, B., Moniot, M., Nourrisson, C., Augereau, O., Emond, J.P., Belkacem-Belkaki, G., Bacri, J.L., Berthelot, G., Dalle, F., Vallee, E., Bizet, J., Noussair, L., Herrmann, J.L., Maubon, D., Brocard, C., Guiffault, P., Layet, A., Morel, A., Angoulvant, A., Penn, P., Gigandon, A., Sendid, B., Cornu, M., Darde, M.L., Jaccard, A., Bouteille, B., Azjenberg, D., Prades, N., Bienvenu, A.L., Benoit-Cattin, T., Fiacre, A., Levy, S., Pitsch, A., Kiefer, M.H., Debourgogne, A., Moquet, O., Colot, J., Courtellemont, L., Poisson, D., Laurens, V., Kauffmann-Lacroix, C., Martres, P., Gargala, G., Godineau, N., Picot, S., Chassagne, C., Djibo, N., Devallière, R., Sabou, M., Camin-Ravenne, A.M., Bissuel, F., Janvier, F., Aubert, X., Chadapaud, S., Delbeck, X., Lafeuillade, A., Raoult, X., Baclet, V., Coignard, C., Mouton, Y., Ravaux, I., Eloy, C., Fur, A., Rezzouk, L., Mazards, E., Eloy, O., Chachaty, E., Mihaila, L., Dellion, S., Patey, O., Thouvenot, A., Limousin, L., Paugam, A., Desplaces, N., Raguin, G., Sitterlé, E., Blaize, M., Gits-Muselli, M., Hennequin, C., Poirot, J.L., Bretagne, S., Lacroix, Claire, Hamane, Samia, Paccoud, Olivier, Desnos-Ollivier, Marie, Persat, Florence, Demar, Magalie, Boukris-Sitbon, Karine, Bellanger, Anne-Pauline, Bonhomme, Julie, Bonnal, Christine, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Cassaing, Sophie, Cateau, Estelle, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, Gabriel, Frederic, Gangneux, Jean-Pierre, Guitard, Juliette, Hasseine, Lilia, Huguenin, Antoine, Le Gal, Solène, Letscher-Bru, Valérie, Mahinc, Caroline, Morio, Florent, Nicolas, Muriel, Poirier, Philippe, Ranque, Stéphane, Roosen, Gabrielle, Rouges, Célia, Roux, Anne-Laure, Sasso, Milène, Alanio, Alexandre, Lortholary, Olivier, and Lanternier, Fanny

Published
2024
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5. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)

Author

Paccoud, Olivier, primary, Desnos-Ollivier, Marie, additional, Persat, Florence, additional, Demar, Magalie, additional, Boukris-Sitbon, Karine, additional, Bellanger, Anne-Pauline, additional, Bonhomme, Julie, additional, Bonnal, Christine, additional, Botterel, Françoise, additional, Bougnoux, Marie-Elisabeth, additional, Brun, Sophie, additional, Cassaing, Sophie, additional, Cateau, Estelle, additional, Chouaki, Taieb, additional, Cornet, Muriel, additional, Dannaoui, Eric, additional, Desbois-Nogard, Nicole, additional, Durieux, Marie-Fleur, additional, Favennec, Loïc, additional, Fekkar, Arnaud, additional, Gabriel, Frederic, additional, Gangneux, Jean-Pierre, additional, Guitard, Juliette, additional, Hasseine, Lilia, additional, Huguenin, Antoine, additional, Le Gal, Solène, additional, Letscher-Bru, Valérie, additional, Mahinc, Caroline, additional, Morio, Florent, additional, Nicolas, Muriel, additional, Poirier, Philippe, additional, Ranque, Stéphane, additional, Roosen, Gabrielle, additional, Rouges, Célia, additional, Roux, Anne-Laure, additional, Sasso, Milène, additional, Alanio, Alexandre, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, Brieu, N., additional, Durand, C., additional, Bertei, D., additional, Bouchara, J.P., additional, Pihet, M., additional, Bland, S., additional, Bru (Annecy), J.P., additional, Pulik, M., additional, Le Turdu, F., additional, Lefrand, C, H., additional, Ferrand, M., additional, Larrouy, M., additional, Millon, L., additional, Delhaes, L., additional, Imbert, S., additional, Accoceberry, I., additional, Bachelier, M.N., additional, Nevez, G., additional, Quinio, D., additional, Le Coustumier, A., additional, Carmagnol, F., additional, Rivière, B., additional, Boex, P., additional, Podac, B., additional, Moniot, M., additional, Nourrisson, C., additional, Augereau, O., additional, Emond, J.P., additional, Belkacem-Belkaki, G., additional, Bacri, J.L., additional, Berthelot, G., additional, Dalle, F., additional, Vallee, E., additional, Bizet, J., additional, Noussair, L., additional, Herrmann, J.L., additional, Maubon, D., additional, Brocard, C., additional, Guiffault, P., additional, Layet, A., additional, Morel, A., additional, Angoulvant, A., additional, Penn, P., additional, Gigandon, A., additional, Sendid, B., additional, Cornu, M., additional, Darde, M.L., additional, Jaccard, A., additional, Bouteille, B., additional, Azjenberg, D., additional, Prades, N., additional, Bienvenu, A.L., additional, Benoit-Cattin, T., additional, Fiacre, A., additional, Levy, S., additional, Pitsch, A., additional, Kiefer, M.H., additional, Debourgogne, A., additional, Moquet, O., additional, Colot, J., additional, Courtellemont, L., additional, Poisson, D., additional, Laurens, V., additional, Kauffmann-Lacroix, C., additional, Martres, P., additional, Gargala, G., additional, Godineau, N., additional, Picot, S., additional, Chassagne, C., additional, Djibo, N., additional, Devallière, R., additional, Sabou, M., additional, Camin-Ravenne, A.M., additional, Bissuel, F., additional, Janvier, F., additional, Aubert, X., additional, Chadapaud, S., additional, Delbeck, X., additional, Lafeuillade, A., additional, Raoult, X., additional, Baclet, V., additional, Coignard, C., additional, Mouton, Y., additional, Ravaux, I., additional, Eloy, C., additional, Fur, A., additional, Rezzouk, L., additional, Mazards, E., additional, Eloy, O., additional, Chachaty, E., additional, Mihaila, L., additional, Dellion, S., additional, Patey, O., additional, Thouvenot, A., additional, Limousin, L., additional, Paugam, A., additional, Desplaces, N., additional, Raguin, G., additional, Sitterlé, E., additional, Blaize, M., additional, Gits-Muselli, M., additional, Hennequin, C., additional, Poirot, J.L., additional, Bretagne, S., additional, Lacroix, Claire, additional, and Hamane, Samia, additional

Published
2024
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6. Spectrum of Pulmonary Aspergillosis in Hyper-IgE Syndrome with Autosomal-Dominant STAT3 Deficiency

Author

Coignard, H., Amazzough, K., Suarez, F., Blanche, S., Sendid, B., Cornu, M., Bervar, J.F., Deschildre, A., Wemeau, L., Fieschi, C., Alanio, A., Menetrey, C., Senechal, A., Ader, F., Tattevin, P., Pison, C., Grandiere-Perez, L., Garcia-Hermoso, D., Botterel-Chartier, F., Duréault, Amélie, Tcherakian, Colas, Poiree, Sylvain, Catherinot, Emilie, Danion, François, Jouvion, Grégory, Bougnoux, Marie Elisabeth, Mahlaoui, Nizar, Givel, Claire, Castelle, Martin, Picard, Capucine, Chansdesris, Marie Olivia, Lortholary, Olivier, and Lanternier, Fanny

Published
2019
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11. Identification of fungal trehalose for the diagnosis of invasive candidiasis by mass spectrometry

Author

Mery, A. (Alexandre), Jawhara, S. (Samir), Francois, N. (Nadine), Cornu, M. (Marjorie), Poissy, J. (Julien), Martinez-Esparza, M. (Maria), Poulain, D. (Daniel), Sendid, B. (Boualem), Guerardel, Y. (Yann), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Universidad de Murcia, Gifu University, Université de Lille, CNRS, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286, and Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]

Subjects
Fungal infection, Serum, Biophysics, Candidiasis, Trehalose, Biochemistry, Mass Spectrometry, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Diagnosis, Candida albicans, Humans, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Candidiasis, Invasive, Molecular Biology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

International audience; The rapidity of the diagnosis of invasive candidiasis (IC) is crucial to allow the early introduction of antifungal therapy that dramatically increases the survival rate of patients. Early diagnosis is unfortunately often delayed because Candida blood culture, the gold standard diagnostic test, is positive in only 50% of cases of IC and takes several days to obtain this result. Complementary non-culture-based methods relying on the detection of Candida cell wall polysaccharides in the serum, β-glucans and mannans, by enzymatic and immunological reagents have been successfully developed to allow a more efficient patients care. We have previously demonstrated that detection of circulating glycans by mass spectrometry could provide a reliable and cost-effective early diagnosis method called MS-DS for Mass Spectrometry of Di-Saccharide. Here, by comparing patient's sera and Candida albicans strains deficient in carbohydrates synthesis, we demonstrate that trehalose derived from fungal metabolism can be specifically targeted by MS-DS to allow early diagnosis. In particular, the use of C. albicans strains deficient in the synthesis of trehalose synthesizing enzymes Tps1 and Tps2 show that MS-DS results were correlated to the metabolism of trehalose. Finally, we demonstrate that the performance of the IC diagnosis can be significantly improved by using high resolution mass spectrometry, which opens new perspectives in the management of the disease.

Published
2021

14. Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group

Author

Rocchi, S, Scherer, E, Mengoli, C, Alanio, A, Botterel, F, Bougnoux, M, Bretagne, S, Cogliati, M, Cornu, M, Dalle, Frédéric, Damiani, Céline, Denis, J, Fuchs, S, Gits-Muselli, M, Hagen, F, Halliday, C, Hare, R, Iriart, Xavier, Klaassen, C, Lackner, M, Lengerova, M, Letscher-Bru, V, Morio, F, Nourrisson, C, Posch, W, Sendid, B, Springer, J, Willinger, B, White, P, Barnes, R, Cruciani, M, Donnelly, J, Loeffler, J, Millon, L, Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Università degli Studi di Padova = University of Padua (Unipd), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris] (IP), Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal, Université Paris Cité (UPCité), Université Paris-Est Créteil Val-de-Marne - Faculté de médecine (UPEC Médecine), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), École nationale vétérinaire - Alfort (ENVA), CHU Henri Mondor [Créteil], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Università degli Studi di Milano = University of Milan (UNIMI), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire de parasitologie mycologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Vin Aliment Microbiologie et Stress (VAlMiS), Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire de parasitologie et de mycologie médicales [CHU Amiens], CHU Amiens-Picardie, Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Laboratoire de Parasitologie et de Mycologie Médicale [Strasbourg], Les Hôpitaux Universitaires de Strasbourg (HUS), Leopold Franzens Universität Innsbruck - University of Innsbruck, UFR Médecine [Santé] - Université Paris Cité (UFR Médecine UPCité), Univ Utrecht, Westerdijk Fungal Biodivers Inst, Fungal Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands, Partenaires INRAE, University Medical Center [Utrecht], Shanghai Key Laboratory of Molecular Medical Mycology, Changzheng Hospital, Second Military Medical University, Statens Serum Institut [Copenhagen], Service de Parasitologie et Mycologie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), University Hospital Brno, CHU Strasbourg, Centre hospitalier universitaire de Nantes (CHU Nantes), CHU Clermont-Ferrand, University Hospital Wuerzburg / Universitäts­klinikum Würzburg, Medizinische Universität Wien = Medical University of Vienna, Public Health Wales [Cardiff, Royaume uni], University of Texas Health Science Center at San Antonio [San Antonio, Tx, USA], This work was supported by a grant from the French Ministry of Health PHRC (Projet Hospitalier de Recherche Clinique) national-ModiMucor 2014-A00580-47., Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Génomique évolutive, modélisation et santé (CNRS-UMR2000), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université Paris Cité - UFR Médecine [Santé] (UPCité UFR Médecine), CHU Henri Mondor, Biologie des ARN des Pathogènes fongiques - RNA Biology of Fungal Pathogens, Institut Pasteur [Paris]-Université Paris Cité (UPCité), University of Innsbruck, Service de Parasitologie et Mycologie, CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity (BPF), Institut Pasteur [Paris]-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Padova Medical School, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPC), Université Paris Cité - UFR Médecine Paris Centre [Santé] (UPC Médecine Paris Centre), Université Paris Cité (UPC), Institut Pasteur [Paris]-Université Paris Cité (UPC), and Università degli Studi di Milano [Milano] (UNIMI)

Subjects
standardization, MESH: Clinical Laboratory Techniques / instrumentation, MESH: Humans, MESH: Mucorales / genetics, Mucorales PCR, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, circulating DNA, MESH: Molecular Diagnostic Techniques / standards, MESH: Observer Variation, MESH: Mucormycosis / blood, MESH: Reproducibility of Results, MESH: France, interlaboratory assay, MESH: Real-Time Polymerase Chain Reaction / standards, MESH: Hospitals, University / statistics & numerical data, MESH: Clinical Laboratory Techniques / standards, MESH: DNA, Fungal / genetics, MESH: Clinical Laboratory Techniques / methods, MESH: Mucormycosis / diagnosis, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77-100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis.

Published
2021

15. Dictionnaire des biens communs

Author

Cornu, M., Orsi, Fabienne, Rochfeld, J., Laboratoire Population-Environnement-Développement (LPED), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut des Sciences sociales du Politique (ISP), École normale supérieure - Cachan (ENS Cachan)-Université Paris Nanterre (UPN)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche juridique de la Sorbonne André Tunc (IRJS), Université Paris 1 Panthéon-Sorbonne (UP1), HORIZON, IRD, and École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Nanterre (UPN)

Subjects
Démocratie, Patrimoines, [SHS.DROIT]Humanities and Social Sciences/Law, [SHS.DROIT] Humanities and Social Sciences/Law, [SHS.ECO] Humanities and Social Sciences/Economics and Finance, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, Biens communs, [SHS.SCIPO]Humanities and Social Sciences/Political science
Abstract

National audience; Que sont les « communs », les « biens communs », les « patrimoines communs », les « choses communes » ? Ces notions sont fortement mobilisées aujourd’hui dans nombre de disciplines des sciences sociales et actions de citoyens. Leur poussée traduit une évolution des pratiques sociales : sous la pression de la crise écologique et de la transformation numérique notamment, les biens seraient davantage mis en partage. Logiciel libre, encyclopédie et habitat participatif, vélos ou voitures en usage successif, entreprise qui serait le « bien commun » de toutes les parties prenantes : les notions des communs intéressent les domaines de la culture, de la protection de l’environnement, de l’urbanisme, de la santé, de l’innovation, du travail, etc. La mobilisation est intense car elles autorisent à penser le changement social sur la base d’un réinvestissement du collectif, des communautés, de l’usage et du partage. Elles permettent de proposer des réinterprétations des valeurs fondatrices des sociétés contemporaines tels le rôle de l’État, de la propriété et des formes d’expression de la démocratie. Elles appellent à la fois une réflexion théorique, un débat politique et se concrétisent dans des expériences citoyennes. Ce dictionnaire, à mi-chemin entre le vocabulaire et l’encyclopédie, se veut un outil de compréhension de l’ensemble de ces phénomènes.

Published
2020

16. Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group

Author

Rocchi, S, Scherer, E, Mengoli, C, Alanio, A, Botterel, F, Bougnoux, M E, Bretagne, S, Cogliati, M, Cornu, M, Dalle, F, Damiani, C, Denis, J, Fuchs, S, Gits-Muselli, M, Hagen, F, Halliday, C, Hare, R, Iriart, X, Klaassen, C, Lackner, M, Lengerova, M, Letscher-Bru, V, Morio, F, Nourrisson, C, Posch, W, Sendid, B, Springer, J, Willinger, B, White, P L, Barnes, R A, Cruciani, M, Donnelly, J P, Loeffler, J, Millon, L, Rocchi, S, Scherer, E, Mengoli, C, Alanio, A, Botterel, F, Bougnoux, M E, Bretagne, S, Cogliati, M, Cornu, M, Dalle, F, Damiani, C, Denis, J, Fuchs, S, Gits-Muselli, M, Hagen, F, Halliday, C, Hare, R, Iriart, X, Klaassen, C, Lackner, M, Lengerova, M, Letscher-Bru, V, Morio, F, Nourrisson, C, Posch, W, Sendid, B, Springer, J, Willinger, B, White, P L, Barnes, R A, Cruciani, M, Donnelly, J P, Loeffler, J, and Millon, L

Abstract

Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77-100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis.

Published
2020

17. Correction for Cornu et al., “Evaluation of mass spectrometry-based detection of panfungal serum disaccharide for diagnosis of invasive fungal infections: Results from a collaborative study involving six European clinical centers”. (J Clin Microbiol (2019) 57, 5 (e01867-18) DOI: 10.1128/JCM.01867-18)

Author

Cornu, M., Sendid, B., Mery, A., François, N., Mikulska, M., Letscher-Bru, V., De Carolis, Elena, Damonti, L., Titecat, M., Bochud, P. Y., Alanio, A., Sanguinetti, Maurizio, Viscoli, C., Herbrecht, R., Guerardel, Y., Poulaina, D., de Carolis E. (ORCID:0000-0003-4757-7256), Sanguinetti M. (ORCID:0000-0002-9780-7059), Cornu, M., Sendid, B., Mery, A., François, N., Mikulska, M., Letscher-Bru, V., De Carolis, Elena, Damonti, L., Titecat, M., Bochud, P. Y., Alanio, A., Sanguinetti, Maurizio, Viscoli, C., Herbrecht, R., Guerardel, Y., Poulaina, D., de Carolis E. (ORCID:0000-0003-4757-7256), and Sanguinetti M. (ORCID:0000-0002-9780-7059)

Abstract

No abstract

Published
2020

40. Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group

Author

Rocchi, S, primary, Scherer, E, additional, Mengoli, C, additional, Alanio, A, additional, Botterel, F, additional, Bougnoux, M E, additional, Bretagne, S, additional, Cogliati, M, additional, Cornu, M, additional, Dalle, F, additional, Damiani, C, additional, Denis, J, additional, Fuchs, S, additional, Gits-Muselli, M, additional, Hagen, F, additional, Halliday, C, additional, Hare, R, additional, Iriart, X, additional, Klaassen, C, additional, Lackner, M, additional, Lengerova, M, additional, Letscher-Bru, V, additional, Morio, F, additional, Nourrisson, C, additional, Posch, W, additional, Sendid, B, additional, Springer, J, additional, Willinger, B, additional, White, P L, additional, Barnes, R A, additional, Cruciani, M, additional, Donnelly, J P, additional, Loeffler, J, additional, and Millon, L, additional

Published
2020
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