Search

The segmentation of cells and neurites in microscopy images of neuronal networks provides valuable quantitative information about neuron growth and neuronal differentiation, including the number of cells, neurites, neurite length and neurite orientation. This information is essential for assessing the development of neuronal networks in response to extracellular stimuli, which is useful for studying neuronal structures, for example, the study of neurodegenerative diseases and pharmaceuticals. However, automatic and accurate analysis of neuronal structures from phase contrast images has remained challenging. To address this, we have developed NeuroQuantify, an open-source software that uses deep learning to efficiently and quickly segment cells and neurites in phase contrast microscopy images. NeuroQuantify offers several key features: (i) automatic detection of cells and neurites; (ii) post-processing of the images for the quantitative neurite length measurement based on segmentation of phase contrast microscopy images, and (iii) identification of neurite orientations. The user-friendly NeuroQuantify software can be installed and freely downloaded from GitHub https://github.com/StanleyZ0528/neural-image-segmentation.

Further advances in exoplanet detection and characterisation require sampling a diverse population of extrasolar planets. One technique to detect these distant worlds is through the direct detection of their thermal emission. The so-called direct imaging technique, is suitable for observing young planets far from their star. These are very low signal-to-noise-ratio (SNR) measurements and limited ground truth hinders the use of supervised learning approaches. In this paper, we combine deep generative and discriminative models to bypass the issues arising when directly training on real data. We use a Generative Adversarial Network to obtain a suitable dataset for training Convolutional Neural Network classifiers to detect and locate planets across a wide range of SNRs. Tested on artificial data, our detectors exhibit good predictive performance and robustness across SNRs. To demonstrate the limits of the detectors, we provide maps of the precision and recall of the model per pixel of the input image. On real data, the models can re-confirm bright source detections., Comment: 16 Pages, 6 Figures, 3 Tables, Presented in ECML-PKDD 2019