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3. DNA breakage detection-fluorescence in situ hybridization in buccal cells

Author

Cortés-Gutiérrez, E.I, Dávila-Rodríguez, M.I, Fernández García, José Luis, López-Fernández, C., and Gosálvez, J.

Subjects
Peróxido de Hidrógeno, Dose-Response Relationship, Drug, Antioxidantes, Hydrogen Peroxide, buccal cell, Oxidants, Hibridación Fluorescente in Situ, Técnicas Genéticas, Technical Report, Cheek, Mejilla, Daño del ADN, Genetic Techniques, DNA damage, Humans, DNA breakage detection/fluorescence in situ hybridization, Relación Dosis-Respuesta a Droga, In Situ Hybridization, Fluorescence, DNA Damage
Abstract

DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

Published
2012

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