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8. Anionic polysaccharides for stabilization and sustained release of antimicrobial peptides

Author

Casadidio, Cristina, Mayol, Laura, Biondi, Marco, Scuri, Stefania, Cortese, Manuela, Hennink, Wim E, Vermonden, Tina, De Rosa, Giuseppe, Di Martino, Piera, Censi, Roberta, Casadidio, Cristina, Mayol, Laura, Biondi, Marco, Scuri, Stefania, Cortese, Manuela, Hennink, Wim E, Vermonden, Tina, De Rosa, Giuseppe, Di Martino, Piera, and Censi, Roberta

Abstract

Chemical and enzymatic in vivo degradation of antimicrobial peptides represents a major challenge for their therapeutic use to treat bacterial infections. In this work, anionic polysaccharides were investigated for their ability to increase the chemical stability and achieve sustained release of such peptides. The investigated formulations comprised a combination of antimicrobial peptides (vancomycin (VAN) and daptomycin (DAP)) and anionic polysaccharides (xanthan gum (XA), hyaluronic acid (HA), propylene glycol alginate (PGA) and alginic acid (ALG)). VAN dissolved in buffer of pH 7.4 and incubated at 37 °C showed first order degradation kinetics with a reaction rate constant k obs of 5.5 × 10 -2 day -1 corresponding with a half-life of 13.9 days. However, once VAN was present in a XA, HA or PGA-based hydrogel, k obs decreased to (2.1-2.3) × 10 -2 day -1 while k obs was not affected in an alginate hydrogel and a dextran solution (5.4 × 10 -2 and 4.4 × 10 -2 day -1). Under the same conditions, XA and PGA also effectively decreased k obs for DAP (5.6 × 10 -2 day -1), whereas ALG had no effect and HA even increased the degradation rate. These results demonstrate that the investigated polysaccharides (except ALG for both peptides and HA for DAP) slowed down the degradation of VAN and DAP. DSC analysis was used to investigate on polysaccharide ability to bind water molecules. Rheological analysis highlighted that the polysaccharides containing VAN displayed an increase in G' of their formulations, pointing that the peptides interaction act as crosslinker of the polymer chains. The obtained results suggest that the mechanism of stabilization of VAN and DAP against hydrolytic degradation is conferred by electrostatic interactions between the ionizable amine groups of the drugs and the anionic carboxylate groups of the polysaccharides. This, in turn, results in a close proximity of the drugs to the polysaccharide chain, where the water molecules have a lower mobility and

Published
2023

9. Anionic polysaccharides for stabilization and sustained release of antimicrobial peptides

Author

Pharmaceutics, Afd Pharmaceutics, Casadidio, Cristina, Mayol, Laura, Biondi, Marco, Scuri, Stefania, Cortese, Manuela, Hennink, Wim E, Vermonden, Tina, De Rosa, Giuseppe, Di Martino, Piera, Censi, Roberta, Pharmaceutics, Afd Pharmaceutics, Casadidio, Cristina, Mayol, Laura, Biondi, Marco, Scuri, Stefania, Cortese, Manuela, Hennink, Wim E, Vermonden, Tina, De Rosa, Giuseppe, Di Martino, Piera, and Censi, Roberta

Published
2023

11. Chemical, Antioxidant, and Antimicrobial Properties of the Peel and Male Flower By-Products of Four Varieties of Punica granatum L. Cultivated in the Marche Region for Their Use in Cosmetic Products

Author

Gigliobianco, Maria Rosa, primary, Cortese, Manuela, additional, Nannini, Samanta, additional, Di Nicolantonio, Lucrezia, additional, Peregrina, Dolores Vargas, additional, Lupidi, Giulio, additional, Vitali, Luca Agostino, additional, Bocchietto, Elena, additional, Di Martino, Piera, additional, and Censi, Roberta, additional

Published
2022
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13. Dually Cross-Linked Core-Shell Structure Nanohydrogel with Redox–Responsive Degradability for Intracellular Delivery

Author

Deng, Siyuan, primary, Gigliobianco, Maria, additional, Mijit, Emin, additional, Minicucci, Marco, additional, Cortese, Manuela, additional, Campisi, Barbara, additional, Voinovich, Dario, additional, Battistelli, Michela, additional, Salucci, Sara, additional, Gobbi, Pietro, additional, Lupidi, Giulio, additional, Zambito, Giorgia, additional, Mezzanotte, Laura, additional, Censi, Roberta, additional, and Di Martino, Piera, additional

Published
2021
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14. Dually cross-linked core-shell structure nanohydrogel with redox–responsive degradability for intracellular delivery

Author

Deng, Siyuan, Gigliobianco, Maria Rosa, Mijiti, Yimin, Minicucci, Marco, Cortese, Manuela, Campisi, Barbara, Voinovich, Dario, Battistelli, Michela, Salucci, Sara, Gobbi, Pietro, Lupidi, Giulio, Zambito, Giorgia, Mezzanotte, Laura, Censi, Roberta, Di Martino, Piera, Deng, Siyuan, Gigliobianco, Maria Rosa, Mijiti, Yimin, Minicucci, Marco, Cortese, Manuela, Campisi, Barbara, Voinovich, Dario, Battistelli, Michela, Salucci, Sara, Gobbi, Pietro, Lupidi, Giulio, Zambito, Giorgia, Mezzanotte, Laura, Censi, Roberta, and Di Martino, Piera

Abstract

A redox-responsive nanocarrier is a promising strategy for the intracellular drug release be-cause it protects the payload, prevents its undesirable leakage during extracellular transport, and favors site-specific drug delivery. In this study, we developed a novel redox responsive core-shell structure nanohydrogel prepared by a water in oil nanoemulsion method using two biocompatible synthetic polymers: vinyl sulfonated poly(N-(2-hydroxypropyl) methacrylamide mono/dilactate)-polyethylene glycol-poly(N-(2-hydroxypropyl) methacrylamide mono/dilactate) triblock copolymer, and thiolated hyaluronic acid. The influence on the nanohydrogel particle size and distribution of formulation parameters was investigated by a three-level full factorial design to optimize the preparation condi-tions. The surface and core-shell morphology of the nanohydrogel were observed by scanning electron microscope, transmission electron microscopy, and further confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy from the standpoint of chemical composition. The redox-responsive biodegradability of the nanohydrogel in reducing environments was determined using glutathione as reducing agent. A nanohydrogel with particle size around 250 nm and polydispersity index around 0.1 is characterized by a thermosensitive shell which jellifies at body temperature and crosslinks at the interface of a redox-responsive hyaluronic acid core via the Michael addition reaction. The nanohydrogel showed good encapsulation efficiency for model macromolecules of different molecular weight (93% for cytochrome C, 47% for horseradish peroxidase, and 90% for bovine serum albumin), capacity to retain the peroxidase-like enzymatic activity (around 90%) of cytochrome C and horseradish peroxidase, and specific redox-responsive release behavior. Additionally, the nanohydrogel exhibited excellent cytocompatibility and internalization efficiency into macrophages. Therefore, the developed core-shell struc

Published
2021

15. Development of New Extracts of Crocus sativus L. By-Product from Two Different Italian Regions as New Potential Active Ingredient in Cosmetic Formulations

Author

Gigliobianco, Maria Rosa, primary, Cortese, Manuela, additional, Peregrina, Dolores Vargas, additional, Villa, Carla, additional, Lupidi, Giulio, additional, Pruccoli, Letizia, additional, Angeloni, Cristina, additional, Tarozzi, Andrea, additional, Censi, Roberta, additional, and Di Martino, Piera, additional

Published
2021
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24. Thermosensitive hybrid hydrogels for the controlled release of bioactive vancomycin in the treatment of orthopaedic implant infections

Author

Afd Pharmaceutics, Pharmaceutics, Censi, Roberta, Casadidio, Cristina, Dubbini, Alessandra, Cortese, Manuela, Scuri, Stefania, Grappasonni, Iolanda, Golob, Samuel, Vojnovic, Dario, Sabbieti, Maria Giovanna, Agas, Dimitrios, Di Martino, Piera, Afd Pharmaceutics, Pharmaceutics, Censi, Roberta, Casadidio, Cristina, Dubbini, Alessandra, Cortese, Manuela, Scuri, Stefania, Grappasonni, Iolanda, Golob, Samuel, Vojnovic, Dario, Sabbieti, Maria Giovanna, Agas, Dimitrios, and Di Martino, Piera

Published
2019

33. SPME-GC-MS analysis of commercial henna samples (Lawsonia inermis L.)

Author

Ricciutelli, Massimo, Maggi, Filippo, Mengoni, Tamara, Peregrina, Dolores Vargas, Censi, Roberta, Cortese, Manuela, and Martino, Piera Di

Abstract

The aim of this work was to provide a characterisation of volatile constituents from different commercial batches of henna (Lawsonia inermis) leaves of different geographic origin. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) was used for the purpose. A total of 72 components were identified by GC–MS in the headspace of different henna samples which proved to differ considerably from each other, because they were characterised by different classes of components, mainly aliphatic compounds (9.0–64.7%), terpenoids (5.8–45.5%) and aromatics (7.9–45.2%), with alkanes (0.9–18.5%), aldehydes (2.1–18.8%) and carboxylic acids (3.1–29.3%), monoterpenes (3.4–30.0%) and sesquiterpenes (0.8–23.7%) and phenyl propanoids (0.6–43.1%), being the most abundant, respectively. Major representatives of these groups were n-hexadecane (0.5–4.7%), (2E)-hexenal (0.5–11.7%) and acetic acid (2.8–24.5%), limonene (0.8–14.7%), carvol (3.8–7.1%), geranyl acetone (1.4–7.9%) and (E)-caryophyllene (3.3–8.4%), and (E)-anethole (0.6–35.0%), respectively. We assume that factors such as the manufacturing process, the storage conditions and the different geographic origin of the samples may contribute to such variability.

Published
2015
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39. Development and application of a UHPLC-MS/MS method for the simultaneous determination of 17 steroidal hormones in equine serum.

Author

Genangeli, Michele, Caprioli, Giovanni, Cortese, Manuela, Laus, Fulvio, Matteucci, Mara, Petrelli, Riccardo, Ricciutelli, Massimo, Sagratini, Gianni, Sartori, Stefano, and Vittori, Sauro

Subjects
LIQUID chromatography-mass spectrometry, STEROID hormones, METABOLITES, SERUM, CORTICOSTERONE
Abstract

A new, fast and simple analytical method that is able to identify and quantify simultaneously 17 steroid hormones and metabolites (pregnenolone, 17-OH-pregnenolone, progesterone, 17-OH-progesterone, androsterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, testosterone, cortisol, corticosterone, aldosterone, 11-deoxycortisol, 11-deoxycorticosterone, dihydrotestosterone, estrone and estradiol) has been developed in equine serum using the ultrahigh- performance liquid chromatography-tandem mass spectrometry technique. A total of 400 μl of sample was deproteinized with 1000 μl of acetonitrile, evaporated, restored with 50 μl of a solution of 25% methanol and injected in ultrahigh- performance liquid chromatography-tandem mass spectrometry triple quadrupole. The recovery percentage obtained by spiking the matrix at two different concentrations with a standard mixture of steroid hormones was in all cases higher than 85.60% and with the percentage of coefficient of variation lower than 8.37%. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.9922-0.9986, and the limits of detection and limits of quantification were in the range of 0.002-2 and 0.0055-5.5 ngml-1, respectively. The detected limit of quantification for testosterone (i.e. 50pgml-1) is twofold lowerwith respect to its threshold admitted in geldings plasma (100pgml-1 free testosterone). The high sensitivity and the quantitative aspect of the method permitted to detect most of the steroids in equine serum. Once validated, the method was used to quantify 17 steroid hormones in mare, stallion and gelding serum samples. The main steroids detected were corticosterone (range 37.25-51.26 ngml-1) and cortisol (range 32.57-52.24 ngml-1), followed by 17-OH-pregnenolone, dihydrotestosterone and pregnenolone. [ABSTRACT FROM AUTHOR]

Published
2017
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40. Essential oil chemotypification and secretory structures of the neglected vegetable Smyrnium olusatrum L. (Apiaceae) growing in central Italy

Author

Maggi, Filippo, primary, Papa, Fabrizio, additional, Giuliani, Claudia, additional, Maleci Bini, Laura, additional, Venditti, Alessandro, additional, Bianco, Armandodoriano, additional, Nicoletti, Marcello, additional, Iannarelli, Romilde, additional, Caprioli, Giovanni, additional, Sagratini, Gianni, additional, Cortese, Manuela, additional, Ricciutelli, Massimo, additional, and Vittori, Sauro, additional

Published
2014
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44. SPME-GC-MS analysis of commercial henna samples ( Lawsonia inermis L.).

Author

Mengoni, Tamara, Vargas Peregrina, Dolores, Censi, Roberta, Cortese, Manuela, Ricciutelli, Massimo, Maggi, Filippo, and Di Martino, Piera

Abstract

The aim of this work was to provide a characterisation of volatile constituents from different commercial batches of henna (Lawsonia inermis) leaves of different geographic origin. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) was used for the purpose. A total of 72 components were identified by GC–MS in the headspace of different henna samples which proved to differ considerably from each other, because they were characterised by different classes of components, mainly aliphatic compounds (9.0–64.7%), terpenoids (5.8–45.5%) and aromatics (7.9–45.2%), with alkanes (0.9–18.5%), aldehydes (2.1–18.8%) and carboxylic acids (3.1–29.3%), monoterpenes (3.4–30.0%) and sesquiterpenes (0.8–23.7%) and phenyl propanoids (0.6–43.1%), being the most abundant, respectively. Major representatives of these groups weren-hexadecane (0.5–4.7%), (2E)-hexenal (0.5–11.7%) and acetic acid (2.8–24.5%), limonene (0.8–14.7%), carvol (3.8–7.1%), geranyl acetone (1.4–7.9%) and (E)-caryophyllene (3.3–8.4%), and (E)-anethole (0.6–35.0%), respectively. We assume that factors such as the manufacturing process, the storage conditions and the different geographic origin of the samples may contribute to such variability. [ABSTRACT FROM PUBLISHER]

Published
2016
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46. Contributors

Author

Abramovič, Helena, Aggarwal, Ramesh Kumar, Akaffou, Sélastique, Alonzo, Elena, Alves, Rita C., Alves, Flávia Maria, Amato, Angélica, Antonio, Andrea Gonçalves, de Assunção, Laélia Soares, Anty, Rodolphe, Arisseto, Adriana Pavesi, Ashihara, Hiroshi, Azevedo, Luciana, Baggio Aguiar, Flávio Henrique, Bagusat, Christiana, Balzano, Michele, Bandosz, Teresa J., Barreca, Davide, Barroso, M. Fátima, Beaudoin, Marie-Soleil, Beghi, Ettore, Bell, Iris R., Bellocco, Ersilia, Bergman, Michael, Bessler, Hanna, Bode, Ann M., Bootzin, Richard R., Borges, Fernanda, Branco, Ana F., Burdan, Franciszek, Bytof, Gerhard, Cai, Li, Cao, Chuanhai, Caprioli, Giovanni, Cárdenas, Casimiro, Carlomagno, Gianfranco, Carvalho, Dayene do Carmo, Casal, Susana, Catalano, Daniela, Cavalli, Lodovica, Celeghini, Renata M.S., Cesarino, Igor, Chen, Chien-Chia, Chen, Chien-Wen, Chiang, Hsiu-Mei, Chu, Li, Coimbra, Manuel A., Conde, Sílvia Vilares, Cortese, Manuela, Costa, Nathália Carvalho, Cousins, Jennifer, Couturon, Emmanuel, Cowan, Theresa, Crouzillat, Dominique, Cruz, Diana, Cunha, Sara, da Costa, Teresa H.M., de Almeida Paula, Thiago, de Cássia Soares da Silva, Marliane, De Caterina, Raffaele, De Grazia, Sara, de Kochko, Alexandre, de Toledo Benassi, Marta, Schmidt de Magalhães, Cristiana, de Oliveira Petkowicz, Carmen L., Debien, Isabel C.N., Delerue-Matos, Cristina, Deus, Cláudia, Devasia, Jeena, di Giuseppe, Romina, Djaldetti, Meir, Domingues, M. Rosário M., Dong, Zigang, Dorea, José G., Duarte, Giselle, Duarte, Abel J., El Omri, Abdelfatteh, Eloy Dias, Rafael Carlos, Enga, Kristin F., Estruch, Ramon, Farah, Adriana, Ferk, Franziska, Fernandes, Flávio Lemes, Ficarra, Silvana, Franca, Adriana S., Franke, Andreas G., Fredholm, B.B., Frega, Natale G., Freitas-Silva, Otniel, Galeone, Carlotta, Galluzzi Bizzo, Maria Letícia, Galtieri, Antonio, Gebara, Valerie, Genkinger, Jeanine M., Giardina, Bruno, Giussani, Giorgia, Goldemberg, Daniel Cohen, González-García, María Begoña, Goto, Masao, Goya, Luis, Graham, Terry E., Guarino, Maria Pedro, Guyot, Romain, Hamon, Perla, Hamon, Serge, Han, Xiaolong, Han, Junkyu, Hansen, John-Bjarne, Hirano, Takeshi, Hoang, Van Dong, Huang, Ya-Han, Igarashi, Kiharu, Imatoh, Takuya, Inouye, Kuniyo, Isoda, Hiroko, Iwahashi, Hideo, Jayarama, Jhang, Jhe-Hua, Kalthoff, Sandra, Kanimozhi, G., Kante, Ba, Karifala, Kemp, Julie Ann, Kim, Jiyoung, Kleinwächter, Maik, Knasmüller, Siegfried, Konka, Sarita, La Vecchia, Carlo, Lachenmeier, Dirk W., Lamuela-Raventós, Rosa M., Lara, Diogo R., Lee, Andy H., Lee, Ki Won, Lee, Seong-Gene, Lim, Loong-Tak, Lopes, Everaldo Antônio, Lopes, João A., Lovadino, José Roberto, Lucci, Paolo, Ma, Defu, Ma, Jan-Nan, Ma, Chao-Mei, Magalhães, Luis M., Maia, Lucianne Cople, Mani, C., Martín, Maria Angeles, Martines, Fabio, Martines, Giuseppe F., Mattioli, Anna Vittoria, Mazzafera, Paulo, Medina, Miguel Á., Medina-Remón, Alexander, Megumi Kasuya, Maria Catarina, Meireles, Maria Angela A., Mesias, Marta, Messina, Paolo, Milhazes, Nuno, Mišík, Miroslav, Monleón, Santiago, Moore, Thea, Morales, Francisco J., Moreira, Ana S.P., Morii, Haruki, Moura, Caroline, Mozzon, Massimo, Mussatto, Solange I., Nakashima, Tamiji, Napolitano, Alessandra, Narita, Yusaku, Nersesyan, Armen, Nilsson, Lena Maria, Nogueira, Gislaine C., Nunes, Fernando M., Nunes, Mateus Dias, Nunes Leite Lima, Débora Alves, Ogawa, Masanori, Oliveira, M. Beatriz P.P., Oliveira, Leandro S., Oliveira, Paulo J., Oliveira, Edna Maria Morais, Pacetti, Deborah, Panzella, Lucia, Pariente, Alexandre, Park, Jae B., Parra, Andrés, Páscoa, Ricardo N.M.J., Pastoriza, Silvia, Pereira Bento, Cláudia Braga, Pham, Ngoc M., Pini, Núbia Pavesi, Poopathi, Subbiah, Porto, Adriana Lofrano, Prasad, N.R., Preedy, Victor R., Protasio Pereira, Luiz Filipe, Pupillo, Elisabetta, Quesada, Ana R., Rakotomalala, Jean-Jacques, Ramalhosa, Maria João, Ramos, Sonia, Rangel, António O.S.S., Razafinarivo, Norosoa J., Reis, Caio E.G., Renda, Giulia, Ribeiro, Maria João, Rigoreau, Michel, Rodrigues da Luz, José Maria, Rodrigues dos Reis, Marcelo, Rodrigues Gorri, Student, Jéssica Emiliane, Rostagno, Mauricio A., Ruela de Carvalho, Daniele, Rufián-Henares, José A., Russo, Annamaria, Sacramento, Joana, Sagratini, Gianni, Sakai, Eiko, Salman, Hertzel, Samanidou, Victoria F., de A. Santiago, Manuela Cristina P., Ferreira dos Santos, Thiago, Santos, João R., Sardão, Vilma, Sarraguça, Mafalda Cruz, Sasaki, Kazunori, Scancetti, Luiza Berguinins, Selmar, Dirk, Serafim, Teresa L., Shearer, Jane, Shibamoto, Takayuki, Siljak-Yakovlev, Sonja, Soares, Cristina, de Souza, Andressa Moreira, Speer, Karl, St-Onge, Marie-Pierre, Strassburg, Christian P., Surya Prakash, Nayani, Tagliazucchi, Davide, Takano-Ishikawa, Yuko, Takarada, Jessica Emi, Tamura, Hiroomi, Tang, Wei, Tanokura, Masaru, Tavani, Alessandra, Tejani, Furqan H., Tellone, Ester, Ivamoto, Suzana Tiemi, Tran, Van Dinh, Tresserra-Rimbau, Anna, Trovato, Francesca M., Trovato, Guglielmo M., Tsukuba, Takayuki, Tunnicliffe, Jasmine M., Turati, Federica, Ulrih, Nataša Poklar, Unfer, Vittorio, Uwai, Yuichi, Vaiarelli, Alberto, Vicente, Eduardo, Vieira, Tiago, Vinader-Caerols, Concepción, Vittori, Sauro, Wang, Xiuju, Wang, Peiyu, Wang, Hsiao-Wen, Wei, Feifei, Weikert, Cornelia, Wen, Kuo-Ching, Wirth, Janine, Xu, Qing, Xu, Yibin, Yoshinari, Orie, and Zuo, Jianping

Published
2015
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